MD01G1120100.v1.1

Domain of unknown function (DUF4378)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0006066
Physical Location & Seq
Reverse (-)
23362761 .. 23363896
1136 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1120100.v1.1.491

Sequence Viewer

Length: 699 bp
ATGACAATTCTTCCAGTCATGGCACAGATGCTTCAGTTCAACAGGTTTGTTGTTTCTTTCTTTAGAAGACAGGAAACATCCACTGAAGACAAAAAAGAAAGTCCATTTCTTTTGCACTGCTCCACCACAGAATCAGATTGTATGGCGAGCTTGGAGGAGGCTGATAAGCCTAGTCCAGTTTCAGTTCTGGAACCAATATTTAGTGGAGAGTGGTCACCTACACCAGAATTCCCGCAAAGGATGAATGTTGACATCACCGCGGAGATAGGTTTTTATGGTAGGAACTTGACAATGGACTTGAGCACCTGGTGCCCTCCGGAATGGCCAGTAAGCCTTGCTGTCTTTGAGACCCTGGAGAAGAAGTTTGGTGATCAGGCATCATGGAAAAGGTCGGACATGAGGCTTTTGTTTGACCGTATAAATGCAGGGTTGATGGAGATTTTCGAGCCTTGTATGGGTGTTCCCACATGGACAAAGCCCGTGTCTAGAAGGATTAGATCCACGCCAAGTGAGGAGATGATAGAGGAAGATCTATGGATGTTGCTTGTTAGCCAAGAGAAGGAAACAGGATGGGACACCACTGAAAAGGCGCTAGGAAGGGAGATTGAGCTAGATTTAGGAGATGATATTGATGGTATTGGTAGAGAAATTGAGAGATTTTTGTTCGATAAGCTCATAGCGGAGTTTGTTACCGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378 VARLMGL

Protein Analysis

233

Amino Acids

26.74

Weight (kDa)

4.49

Isoelectric Point (pI)

65.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4378 PF14309 86 - 228 2.5e-24 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 309
AccII CGCG 1 cut(s) 260
AccIII TCCGGA 1 cut(s) 316
AciI CCGC 4 cut(s) 233, 258, 260, 680
AclWI GGATC 1 cut(s) 492
AcoI YGGCCR 1 cut(s) 323
AcsI RAATTY 1 cut(s) 227
AcuI CTGAAG 2 cut(s) 17, 105
AdeI CACNNNGTG 1 cut(s) 309
AfiI CCNNNNNNNGG 2 cut(s) 455, 559
AgsI TTSAA 1 cut(s) 40
AjnI CCWGG 2 cut(s) 305, 351
AluBI AGCT 3 cut(s) 150, 610, 673
AluI AGCT 3 cut(s) 150, 610, 673
Alw21I GWGCWC 1 cut(s) 305
Alw26I GTCTC 1 cut(s) 341
AlwI GGATC 1 cut(s) 492
Aor13HI TCCGGA 1 cut(s) 316
AoxI GGCC 1 cut(s) 323
ApoI RAATTY 1 cut(s) 227
ArsI GACNNNNNNTTYG 2 cut(s) 467, 499
AspLEI GCGC 1 cut(s) 592
AsuHPI GGTGA 3 cut(s) 207, 247, 380
BaeGI GKGCMC 1 cut(s) 314
BalI TGGCCA 1 cut(s) 325
BanI GGYRCC 1 cut(s) 309
BbsI GAAGAC 2 cut(s) 73, 93
Bbv12I GWGCWC 1 cut(s) 305
BccI CCATC 3 cut(s) 427, 564, 626
BciT130I CCWGG 2 cut(s) 307, 353
BclI TGATCA 1 cut(s) 370
BcoDI GTCTC 1 cut(s) 341
BfaI CTAG 4 cut(s) 171, 486, 593, 611
BfoI RGCGCY 1 cut(s) 593
BglII AGATCT 1 cut(s) 529
Bme1390I CCNGG 2 cut(s) 307, 353
BmiI GGNNCC 2 cut(s) 192, 311
BmrFI CCNGG 2 cut(s) 307, 353
BmsI GCATC 2 cut(s) 18, 386
BpiI GAAGAC 2 cut(s) 73, 93
BpmI CTGGAG 1 cut(s) 374
BpuEI CTTGAG 1 cut(s) 319
BsaI GGTCTC 1 cut(s) 341
BsaJI CCNNGG 2 cut(s) 258, 351
BsaWI WCCGGW 1 cut(s) 316
BsaXI ACNNNNNCTCC 2 cut(s) 347, 377
Bsc4I CCNNNNNNNGG 2 cut(s) 455, 559
Bse1I ACTGG 3 cut(s) 14, 176, 326
BseAI TCCGGA 1 cut(s) 316
BseBI CCWGG 2 cut(s) 307, 353
BseDI CCNNGG 2 cut(s) 258, 351
BseGI GGATG 4 cut(s) 77, 246, 543, 575
BseLI CCNNNNNNNGG 2 cut(s) 455, 559
BseNI ACTGG 3 cut(s) 14, 176, 326
BseRI GAGGAG 2 cut(s) 170, 527
BseSI GKGCMC 1 cut(s) 314
Bsh1236I CGCG 1 cut(s) 260
BshFI GGCC 1 cut(s) 325
BshNI GGYRCC 1 cut(s) 309
BsiHKAI GWGCWC 1 cut(s) 305
BsiSI CCGG 1 cut(s) 317
BslFI GGGAC 1 cut(s) 587
BslI CCNNNNNNNGG 2 cut(s) 455, 559
BsmAI GTCTC 1 cut(s) 341
BsmFI GGGAC 1 cut(s) 587
BsnI GGCC 1 cut(s) 325
Bso31I GGTCTC 1 cut(s) 341
Bsp1286I GDGCHC 2 cut(s) 305, 314
Bsp13I TCCGGA 1 cut(s) 316
Bsp143I GATC 3 cut(s) 370, 497, 529
BspACI CCGC 4 cut(s) 233, 258, 260, 680
BspANI GGCC 1 cut(s) 325
BspEI TCCGGA 1 cut(s) 316
BspFNI CGCG 1 cut(s) 260
BspLI GGNNCC 2 cut(s) 192, 311
BspPI GGATC 1 cut(s) 492
BspT107I GGYRCC 1 cut(s) 309
BspTNI GGTCTC 1 cut(s) 341
BsrI ACTGG 3 cut(s) 14, 176, 326
BssECI CCNNGG 2 cut(s) 258, 351
BssMI GATC 3 cut(s) 370, 497, 529
Bst2UI CCWGG 2 cut(s) 307, 353
Bst4CI ACNGT 2 cut(s) 416, 694
BstAPI GCANNNNNTGC 1 cut(s) 309
BstC8I GCNNGC 1 cut(s) 148
BstDSI CCRYGG 1 cut(s) 258
BstEII GGTNACC 1 cut(s) 213
BstF5I GGATG 4 cut(s) 77, 246, 543, 575
BstFNI CGCG 1 cut(s) 260
BstH2I RGCGCY 1 cut(s) 593
BstHHI GCGC 1 cut(s) 592
BstKTI GATC 3 cut(s) 373, 500, 532
BstMAI GTCTC 1 cut(s) 341
BstMBI GATC 3 cut(s) 370, 497, 529
BstMWI GCNNNNNNNGC 1 cut(s) 309
BstNI CCWGG 2 cut(s) 307, 353
BstPI GGTNACC 1 cut(s) 213
BstSCI CCNGG 2 cut(s) 305, 351
BstSLI GKGCMC 1 cut(s) 314
BstUI CGCG 1 cut(s) 260
BstV2I GAAGAC 2 cut(s) 73, 93
BstX2I RGATCY 2 cut(s) 497, 529
BstYI RGATCY 2 cut(s) 497, 529
BsuRI GGCC 1 cut(s) 325
BtgI CCRYGG 1 cut(s) 258
BtsCI GGATG 4 cut(s) 77, 246, 543, 575
BtsI GCAGTG 1 cut(s) 115
BtsIMutI CAGTG 3 cut(s) 81, 115, 579
Cac8I GCNNGC 1 cut(s) 148
CfoI GCGC 1 cut(s) 592
Cfr42I CCGCGG 1 cut(s) 261
CsiI ACCWGGT 1 cut(s) 305
CviAII CATG 4 cut(s) 19, 381, 397, 468
DpnI GATC 3 cut(s) 372, 499, 531
DpnII GATC 3 cut(s) 370, 497, 529
DraIII CACNNNGTG 1 cut(s) 309
EaeI YGGCCR 1 cut(s) 323
Eco31I GGTCTC 1 cut(s) 341
Eco57I CTGAAG 2 cut(s) 17, 105
Eco91I GGTNACC 1 cut(s) 213
EcoO65I GGTNACC 1 cut(s) 213
EcoRI GAATTC 1 cut(s) 227
EcoRII CCWGG 2 cut(s) 305, 351
FaeI CATG 4 cut(s) 22, 384, 400, 471
FaqI GGGAC 1 cut(s) 587
FatI CATG 4 cut(s) 18, 380, 396, 467
FauI CCCGC 1 cut(s) 240
FbaI TGATCA 1 cut(s) 370
FokI GGATG 4 cut(s) 64, 253, 550, 582
FspBI CTAG 4 cut(s) 171, 486, 593, 611
GlaI GCGC 1 cut(s) 591
GsuI CTGGAG 1 cut(s) 374
HaeII RGCGCY 1 cut(s) 593
HaeIII GGCC 1 cut(s) 325
HapII CCGG 1 cut(s) 317
HhaI GCGC 1 cut(s) 592
Hin1II CATG 4 cut(s) 22, 384, 400, 471
Hin6I GCGC 1 cut(s) 590
HinP1I GCGC 1 cut(s) 590
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HinfI GANTC 1 cut(s) 131
HpaII CCGG 1 cut(s) 317
HphI GGTGA 3 cut(s) 207, 247, 380
Hpy166II GTNNAC 1 cut(s) 250
Hpy188I TCNGA 2 cut(s) 136, 394
Hpy188III TCNNGA 3 cut(s) 188, 317, 486
Hpy8I GTNNAC 1 cut(s) 250
HpyAV CCTTC 3 cut(s) 483, 553, 591
HpyCH4III ACNGT 2 cut(s) 416, 694
HpyCH4V TGCA 2 cut(s) 115, 425
HpyF10VI GCNNNNNNNGC 1 cut(s) 309
Hsp92II CATG 4 cut(s) 22, 384, 400, 471
HspAI GCGC 1 cut(s) 590
Kpn2I TCCGGA 1 cut(s) 316
Ksp22I TGATCA 1 cut(s) 370
KspI CCGCGG 1 cut(s) 261
Kzo9I GATC 3 cut(s) 370, 497, 529
LmnI GCTCC 1 cut(s) 125
LweI GCATC 2 cut(s) 18, 386
MabI ACCWGGT 1 cut(s) 305
MaeI CTAG 4 cut(s) 171, 486, 593, 611
MaeIII GTNAC 2 cut(s) 213, 688
MalI GATC 3 cut(s) 372, 499, 531
MboI GATC 3 cut(s) 370, 497, 529
MboII GAAGA 4 cut(s) 78, 98, 370, 539
MflI RGATCY 2 cut(s) 497, 529
MhlI GDGCHC 2 cut(s) 305, 314
MlsI TGGCCA 1 cut(s) 325
MluCI AATT 3 cut(s) 6, 227, 648
MluNI TGGCCA 1 cut(s) 325
MmeI TCCRAC 1 cut(s) 372
MnlI CCTC 6 cut(s) 148, 151, 324, 393, 505, 517
Mox20I TGGCCA 1 cut(s) 325
MroI TCCGGA 1 cut(s) 316
MscI TGGCCA 1 cut(s) 325
Msp20I TGGCCA 1 cut(s) 325
MspA1I CMGCKG 1 cut(s) 260
MspI CCGG 1 cut(s) 317
MspR9I CCNGG 2 cut(s) 307, 353
MvaI CCWGG 2 cut(s) 307, 353
MvnI CGCG 1 cut(s) 260
MwoI GCNNNNNNNGC 1 cut(s) 309
NdeII GATC 3 cut(s) 370, 497, 529
NlaIII CATG 4 cut(s) 22, 384, 400, 471
NlaIV GGNNCC 2 cut(s) 192, 311
NmuCI GTSAC 1 cut(s) 213
PfeI GAWTC 1 cut(s) 131
Psp6I CCWGG 2 cut(s) 305, 351
PspEI GGTNACC 1 cut(s) 213
PspGI CCWGG 2 cut(s) 305, 351
PspN4I GGNNCC 2 cut(s) 192, 311
PsuI RGATCY 2 cut(s) 497, 529
SacII CCGCGG 1 cut(s) 261
Sau3AI GATC 3 cut(s) 370, 497, 529
ScrFI CCNGG 2 cut(s) 307, 353
SduI GDGCHC 2 cut(s) 305, 314
SetI ASST 8 cut(s) 47, 152, 220, 271, 308, 392, 612, 675
SexAI ACCWGGT 1 cut(s) 305
SfaNI GCATC 2 cut(s) 18, 386
Sfr303I CCGCGG 1 cut(s) 261
SgrBI CCGCGG 1 cut(s) 261
SmlI CTYRAG 1 cut(s) 298
SmoI CTYRAG 1 cut(s) 298
Sse9I AATT 3 cut(s) 6, 227, 648
SsiI CCGC 4 cut(s) 233, 258, 260, 680
SspI AATATT 1 cut(s) 198
SspMI CTAG 4 cut(s) 171, 486, 593, 611
StyD4I CCNGG 2 cut(s) 305, 351
TaaI ACNGT 2 cut(s) 416, 694
TaqI TCGA 2 cut(s) 444, 666
TasI AATT 3 cut(s) 6, 227, 648
TfiI GAWTC 1 cut(s) 131
TscAI CASTG 3 cut(s) 88, 122, 586
TseFI GTSAC 1 cut(s) 213
Tsp45I GTSAC 1 cut(s) 213
TspDTI ATGAA 1 cut(s) 257
TspRI CASTG 3 cut(s) 88, 122, 586
XapI RAATTY 1 cut(s) 227
XbaI TCTAGA 1 cut(s) 485
XspI CTAG 4 cut(s) 171, 486, 593, 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.