AT2G42990

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0013813
Physical Location & Seq
Forward (+)
17878944 .. 17880563
1620 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G42990.1

Sequence Viewer

Length: 1053 bp
ATGGCAACACATTATTTGTCACCATCAATATTATGCATTATTTTGACCACTCTTGTCTCCATCGCCGGAGCAAAAATCCCGGCGATTATAGTCTTCGGTGACTCTTCCGTAGATTCCGGCAATAATAACTTCATATCAACAATGGCCAGAGCCAATTTTGAGCCTTACGGTCGTGACTTCCCTGGTGGTCGCGCCACCGGACGCTTTTGTAACGGCCGTCTCTCCTCCGATTTCACTTCCGAGGCTTATGGCCTTAAACCGACCGTGCCAGCCTACCTAGATCCGTCCTATAATATATCTGATTTTGCTACCGGAGTTTGTTTTGCCTCCGCCGGCACCGGTTATGATAATTCCACAGCCGATGTGCTTGGCGTGATTCCGTTATGGAAAGAGGTCGAGTACTTCAAGGAATACCAAAGCAACCTTTCCGCCTATCTCGGCCATCGGAGAGCTGCCAAAATCATCAGAGAATCTCTGTACATAGTAAGCATAGGCACCAACGATTTCCTTGAGAACTACTACACATTACCGGACAGGAGATCTCAATTCAGCATCAGTCAGTACCAAGACTTCCTGGTCGAGATTGCTGAGGTGTTCTTAAAGGACATTTACAGACTTGGAGCTCGGAAAATGTCTTTCACTGGAATCTCACCTATGGGGTGTCTACCGCTGGAGAGAGTGACAAATCTTGACGACCCTTTTAGCTGTGCTCGGAGTTATAATGATTTGGCTGTAGATTTCAATGGGAGATTGAGAAGATTGGTGACAAAACTGAATAGAGAGCTTACTGGAATAAAGATTTATTTCGCTAATCCTTACGATATCATGTGGGATATTGTTACCAAACCTAATCTTTACGGTCTAGAGATATCAAGTTCTGCCTGTTGCGGTACTGGATTGTTCGAGATGGGATTTCTTTGCGGCCAGGACAATCCGTTAACTTGCAGTGATGCCAATAAGTTCGTTTTCTGGGACGCATTTCACCCGACCGAGAGGACTAATCAGATTGTGTCTGATCACTTCTTCAAGCATCTCAAGAATCTGTTTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

350

Amino Acids

39.2

Weight (kDa)

5.9

Isoelectric Point (pI)

30.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 29 - 341 8.2e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013813)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42990
fragaria_vesca FvH4_6g38660 FvH4_6g38660
malus_domestica MD09G1148500.v1.1
prunus_persica Prupe.3G177600_v2.0.a1
pyrus_communis pycom09g06820
rosa_chinensis RchiOBHm_Chr2g0152121
rosa_laevigata RLG00000020610
rosa_multiflora Rmu_sc0000760.1_g000001 Rmu_sc0003043.1_g000023
rosa_roxburghii Rroxscaffold_2G00095910
rosa_rugosa Rorug02G0431200
rosa_samantha Rh2AG492000 Rh2BG504500 Rh2CG478500 Rh2DG515800
rosa_wichuraiana Rw2G040440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 720
AasI GACNNNNNNGTC 1 cut(s) 575
AccB1I GGYRCC 2 cut(s) 335, 494
AccI GTMKAC 1 cut(s) 664
AccII CGCG 1 cut(s) 192
AciI CCGC 5 cut(s) 330, 429, 668, 888, 921
AclWI GGATC 1 cut(s) 275
AcoI YGGCCR 4 cut(s) 144, 214, 439, 922
AfaI GTAC 4 cut(s) 401, 479, 563, 892
AgeI ACCGGT 1 cut(s) 338
AgsI TTSAA 3 cut(s) 406, 742, 1027
AjnI CCWGG 3 cut(s) 181, 573, 924
AluBI AGCT 4 cut(s) 452, 623, 705, 784
AluI AGCT 4 cut(s) 452, 623, 705, 784
Alw21I GWGCWC 2 cut(s) 625, 712
Alw26I GTCTC 2 cut(s) 61, 224
AlwI GGATC 1 cut(s) 275
AoxI GGCC 5 cut(s) 144, 214, 250, 439, 922
ApeKI GCWGC 1 cut(s) 452
AsiGI ACCGGT 1 cut(s) 338
AspLEI GCGC 1 cut(s) 194
AsuC2I CCSGG 1 cut(s) 80
AsuHPI GGTGA 5 cut(s) 12, 110, 642, 775, 974
BalI TGGCCA 1 cut(s) 146
BanI GGYRCC 2 cut(s) 335, 494
BanII GRGCYC 1 cut(s) 625
BarI GAAGNNNNNNTAC 2 cut(s) 554, 586
BbsI GAAGAC 1 cut(s) 85
Bbv12I GWGCWC 2 cut(s) 625, 712
BbvCI CCTCAGC 1 cut(s) 588
BbvI GCAGC 1 cut(s) 439
BccI CCATC 4 cut(s) 31, 68, 450, 901
BceAI ACGGC 2 cut(s) 201, 229
BciT130I CCWGG 3 cut(s) 183, 575, 926
BclI TGATCA 1 cut(s) 1015
BcnI CCSGG 1 cut(s) 80
BcoDI GTCTC 2 cut(s) 61, 224
BfaI CTAG 2 cut(s) 278, 863
BfmI CTRYAG 1 cut(s) 732
BglII AGATCT 1 cut(s) 539
BisI GCNGC 2 cut(s) 453, 922
BlsI GCNGC 2 cut(s) 454, 923
BmcAI AGTACT 1 cut(s) 401
Bme1390I CCNGG 4 cut(s) 80, 183, 575, 926
BmiI GGNNCC 2 cut(s) 337, 496
BmrFI CCNGG 4 cut(s) 80, 183, 575, 926
BmsI GCATC 3 cut(s) 561, 940, 1039
BpiI GAAGAC 1 cut(s) 85
BpmI CTGGAG 1 cut(s) 692
Bpu10I CCTNAGC 1 cut(s) 588
BpuEI CTTGAG 2 cut(s) 530, 1019
BpuMI CCSGG 1 cut(s) 80
BsaJI CCNNGG 2 cut(s) 181, 240
BsaWI WCCGGW 4 cut(s) 197, 311, 338, 529
Bse118I RCCGGY 2 cut(s) 332, 338
Bse1I ACTGG 3 cut(s) 646, 793, 898
BseBI CCWGG 3 cut(s) 183, 575, 926
BseDI CCNNGG 2 cut(s) 181, 240
BseMII CTCAG 1 cut(s) 579
BseNI ACTGG 3 cut(s) 646, 793, 898
BseRI GAGGAG 1 cut(s) 214
BseX3I CGGCCG 1 cut(s) 214
BseXI GCAGC 1 cut(s) 439
Bsh1236I CGCG 1 cut(s) 192
Bsh1285I CGRYCG 4 cut(s) 172, 217, 264, 990
BshFI GGCC 5 cut(s) 146, 216, 252, 441, 924
BshNI GGYRCC 2 cut(s) 335, 494
BshTI ACCGGT 1 cut(s) 338
BsiEI CGRYCG 4 cut(s) 172, 217, 264, 990
BsiHKAI GWGCWC 2 cut(s) 625, 712
BsiSI CCGG 8 cut(s) 66, 80, 117, 198, 312, 333, 339, 530
BslFI GGGAC 1 cut(s) 986
BsmAI GTCTC 2 cut(s) 61, 224
BsmBI CGTCTC 1 cut(s) 224
BsmFI GGGAC 1 cut(s) 986
BsnI GGCC 5 cut(s) 146, 216, 252, 441, 924
Bsp1286I GDGCHC 2 cut(s) 625, 712
Bsp1407I TGTACA 1 cut(s) 477
Bsp143I GATC 3 cut(s) 280, 539, 1015
BspACI CCGC 5 cut(s) 330, 429, 668, 888, 921
BspANI GGCC 5 cut(s) 146, 216, 252, 441, 924
BspCNI CTCAG 1 cut(s) 580
BspFNI CGCG 1 cut(s) 192
BspLI GGNNCC 2 cut(s) 337, 496
BspPI GGATC 1 cut(s) 275
BspT107I GGYRCC 2 cut(s) 335, 494
BsrFI RCCGGY 2 cut(s) 332, 338
BsrGI TGTACA 1 cut(s) 477
BsrI ACTGG 3 cut(s) 646, 793, 898
BssAI RCCGGY 2 cut(s) 332, 338
BssECI CCNNGG 2 cut(s) 181, 240
BssMI GATC 3 cut(s) 280, 539, 1015
Bst2UI CCWGG 3 cut(s) 183, 575, 926
Bst4CI ACNGT 3 cut(s) 170, 265, 860
Bst6I CTCTTC 1 cut(s) 109
BstAUI TGTACA 1 cut(s) 477
BstC8I GCNNGC 2 cut(s) 270, 334
BstDEI CTNAG 1 cut(s) 588
BstFNI CGCG 1 cut(s) 192
BstHHI GCGC 1 cut(s) 194
BstKTI GATC 3 cut(s) 283, 542, 1018
BstMAI GTCTC 2 cut(s) 61, 224
BstMBI GATC 3 cut(s) 280, 539, 1015
BstMCI CGRYCG 4 cut(s) 172, 217, 264, 990
BstNI CCWGG 3 cut(s) 183, 575, 926
BstSCI CCNGG 4 cut(s) 78, 181, 573, 924
BstSFI CTRYAG 1 cut(s) 732
BstUI CGCG 1 cut(s) 192
BstV1I GCAGC 1 cut(s) 439
BstV2I GAAGAC 1 cut(s) 85
BstX2I RGATCY 2 cut(s) 280, 539
BstYI RGATCY 2 cut(s) 280, 539
BstZI CGGCCG 1 cut(s) 214
BsuRI GGCC 5 cut(s) 146, 216, 252, 441, 924
BtgZI GCGATG 1 cut(s) 46
BtsI GCAGTG 1 cut(s) 952
BtsIMutI CAGTG 2 cut(s) 639, 952
Cac8I GCNNGC 2 cut(s) 270, 334
CfoI GCGC 1 cut(s) 194
Cfr10I RCCGGY 2 cut(s) 332, 338
CseI GACGC 2 cut(s) 210, 983
Csp6I GTAC 4 cut(s) 400, 478, 562, 891
CspAI ACCGGT 1 cut(s) 338
CviAII CATG 1 cut(s) 826
CviQI GTAC 4 cut(s) 400, 478, 562, 891
DdeI CTNAG 1 cut(s) 588
DpnI GATC 3 cut(s) 282, 541, 1017
DpnII GATC 3 cut(s) 280, 539, 1015
DrdI GACNNNNNNGTC 1 cut(s) 575
DseDI GACNNNNNNGTC 1 cut(s) 575
EaeI YGGCCR 4 cut(s) 144, 214, 439, 922
EagI CGGCCG 1 cut(s) 214
Eam1104I CTCTTC 1 cut(s) 109
EarI CTCTTC 1 cut(s) 109
EciI GGCGGA 2 cut(s) 319, 418
Ecl136II GAGCTC 1 cut(s) 623
EclXI CGGCCG 1 cut(s) 214
Eco24I GRGCYC 1 cut(s) 625
Eco32I GATATC 2 cut(s) 823, 870
Eco52I CGGCCG 1 cut(s) 214
Eco53kI GAGCTC 1 cut(s) 623
EcoICRI GAGCTC 1 cut(s) 623
EcoRII CCWGG 3 cut(s) 181, 573, 924
EcoRV GATATC 2 cut(s) 823, 870
EcoT22I ATGCAT 1 cut(s) 38
EcoT38I GRGCYC 1 cut(s) 625
Esp3I CGTCTC 1 cut(s) 224
FaeI CATG 1 cut(s) 829
FaqI GGGAC 1 cut(s) 986
FatI CATG 1 cut(s) 825
FbaI TGATCA 1 cut(s) 1015
FblI GTMKAC 1 cut(s) 664
Fnu4HI GCNGC 2 cut(s) 453, 922
FriOI GRGCYC 1 cut(s) 625
Fsp4HI GCNGC 2 cut(s) 453, 922
FspBI CTAG 2 cut(s) 278, 863
GlaI GCGC 1 cut(s) 193
GluI GCNGC 2 cut(s) 453, 922
GsuI CTGGAG 1 cut(s) 692
HaeIII GGCC 5 cut(s) 146, 216, 252, 441, 924
HapII CCGG 8 cut(s) 66, 80, 117, 198, 312, 333, 339, 530
HgaI GACGC 2 cut(s) 210, 983
HhaI GCGC 1 cut(s) 194
Hin1II CATG 1 cut(s) 829
Hin6I GCGC 1 cut(s) 192
HinP1I GCGC 1 cut(s) 192
HincII GTYRAC 1 cut(s) 939
HindII GTYRAC 1 cut(s) 939
HinfI GANTC 6 cut(s) 101, 113, 376, 470, 645, 1039
HpaI GTTAAC 1 cut(s) 939
HpaII CCGG 8 cut(s) 66, 80, 117, 198, 312, 333, 339, 530
HphI GGTGA 5 cut(s) 12, 110, 642, 775, 974
Hpy166II GTNNAC 2 cut(s) 665, 939
Hpy188I TCNGA 9 cut(s) 229, 241, 301, 447, 467, 627, 714, 1005, 1015
Hpy188III TCNNGA 6 cut(s) 173, 580, 689, 863, 904, 1036
Hpy8I GTNNAC 2 cut(s) 665, 939
HpyCH4III ACNGT 3 cut(s) 170, 265, 860
HpyCH4V TGCA 2 cut(s) 36, 945
HpyF3I CTNAG 1 cut(s) 588
Hsp92II CATG 1 cut(s) 829
HspAI GCGC 1 cut(s) 192
KroI GCCGGC 1 cut(s) 332
KroNI GCCGGC 1 cut(s) 334
Ksp22I TGATCA 1 cut(s) 1015
KspAI GTTAAC 1 cut(s) 939
Kzo9I GATC 3 cut(s) 280, 539, 1015
LmnI GCTCC 2 cut(s) 68, 620
Lsp1109I GCAGC 1 cut(s) 439
LweI GCATC 3 cut(s) 561, 940, 1039
MaeI CTAG 2 cut(s) 278, 863
MaeIII GTNAC 7 cut(s) 18, 98, 173, 209, 679, 763, 838
MalI GATC 3 cut(s) 282, 541, 1017
MboI GATC 3 cut(s) 280, 539, 1015
MboII GAAGA 4 cut(s) 85, 96, 768, 1015
MflI RGATCY 2 cut(s) 280, 539
MhlI GDGCHC 2 cut(s) 625, 712
MlsI TGGCCA 1 cut(s) 146
MluCI AATT 3 cut(s) 154, 349, 545
MluNI TGGCCA 1 cut(s) 146
MlyI GAGTC 1 cut(s) 95
MnlI CCTC 6 cut(s) 235, 235, 337, 385, 583, 987
Mox20I TGGCCA 1 cut(s) 146
Mph1103I ATGCAT 1 cut(s) 38
MroNI GCCGGC 1 cut(s) 332
MscI TGGCCA 1 cut(s) 146
MseI TTAA 3 cut(s) 255, 599, 938
Msp20I TGGCCA 1 cut(s) 146
MspA1I CMGCKG 1 cut(s) 670
MspI CCGG 8 cut(s) 66, 80, 117, 198, 312, 333, 339, 530
MspR9I CCNGG 4 cut(s) 80, 183, 575, 926
MvaI CCWGG 3 cut(s) 183, 575, 926
MvnI CGCG 1 cut(s) 192
NaeI GCCGGC 1 cut(s) 334
NciI CCSGG 1 cut(s) 80
NdeII GATC 3 cut(s) 280, 539, 1015
NgoMIV GCCGGC 1 cut(s) 332
NlaIII CATG 1 cut(s) 829
NlaIV GGNNCC 2 cut(s) 337, 496
NmeAIII GCCGAG 1 cut(s) 417
NmuCI GTSAC 5 cut(s) 18, 98, 173, 679, 763
NsiI ATGCAT 1 cut(s) 38
PdiI GCCGGC 1 cut(s) 334
PfeI GAWTC 5 cut(s) 113, 376, 470, 645, 1039
PinAI ACCGGT 1 cut(s) 338
PkrI GCNGC 2 cut(s) 454, 923
PleI GAGTC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 95
PsiI TTATAA 1 cut(s) 720
Psp124BI GAGCTC 1 cut(s) 625
Psp6I CCWGG 3 cut(s) 181, 573, 924
PspGI CCWGG 3 cut(s) 181, 573, 924
PspN4I GGNNCC 2 cut(s) 337, 496
PsuI RGATCY 2 cut(s) 280, 539
RsaI GTAC 4 cut(s) 401, 479, 563, 892
RsaNI GTAC 4 cut(s) 400, 478, 562, 891
SacI GAGCTC 1 cut(s) 625
SaqAI TTAA 3 cut(s) 255, 599, 938
SatI GCNGC 2 cut(s) 453, 922
Sau3AI GATC 3 cut(s) 280, 539, 1015
ScaI AGTACT 1 cut(s) 401
SchI GAGTC 1 cut(s) 95
ScrFI CCNGG 4 cut(s) 80, 183, 575, 926
SduI GDGCHC 2 cut(s) 625, 712
SfaNI GCATC 3 cut(s) 561, 940, 1039
SfcI CTRYAG 1 cut(s) 732
SmlI CTYRAG 2 cut(s) 509, 1034
SmoI CTYRAG 2 cut(s) 509, 1034
Sse9I AATT 3 cut(s) 154, 349, 545
SsiI CCGC 5 cut(s) 330, 429, 668, 888, 921
SspI AATATT 1 cut(s) 30
SspMI CTAG 2 cut(s) 278, 863
SstI GAGCTC 1 cut(s) 625
StyD4I CCNGG 4 cut(s) 78, 181, 573, 924
TaaI ACNGT 3 cut(s) 170, 265, 860
TaqI TCGA 3 cut(s) 396, 579, 903
TaqII GACCGA 1 cut(s) 1004
TasI AATT 3 cut(s) 154, 349, 545
TatI WGTACW 2 cut(s) 399, 477
TauI GCSGC 1 cut(s) 924
TfiI GAWTC 5 cut(s) 113, 376, 470, 645, 1039
Tru1I TTAA 3 cut(s) 255, 599, 938
Tru9I TTAA 3 cut(s) 255, 599, 938
TscAI CASTG 2 cut(s) 646, 952
TseFI GTSAC 5 cut(s) 18, 98, 173, 679, 763
TseI GCWGC 1 cut(s) 452
Tsp45I GTSAC 5 cut(s) 18, 98, 173, 679, 763
TspDTI ATGAA 2 cut(s) 121, 1037
TspGWI ACGGA 4 cut(s) 97, 273, 369, 924
TspRI CASTG 2 cut(s) 646, 952
XbaI TCTAGA 1 cut(s) 862
XmiI GTMKAC 1 cut(s) 664
XspI CTAG 2 cut(s) 278, 863
ZrmI AGTACT 1 cut(s) 401
Zsp2I ATGCAT 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.