MD09G1148500.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0013813
Physical Location & Seq
Reverse (-)
11671413 .. 11673906
2494 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1148500.v1.1.491

Sequence Viewer

Length: 1050 bp
ATGGCAAACTTGTATATTCCCTGGCTTTTCTTAGTTCATACCCTAACCCTTGTTGCCAAATGTAGAGCTAAAGTTCCAGCAGTCATAGTATTTGGAGATTCTTCTGTTGATTCAGGTAACAACAACTTCATTCCTACAATTGCCAGGAGCAACTTCCAGCCTTATGGTCAAGATTTTCCGGGAGGCCAGCCCACCGGGCGGTTCTGCAATGGCCGGATTCCTCCTGACTTCATCTCCGAAGGTCTAGGCTGCAAGCCAATTATACCTGCCTACTTAGATCCAACTCACAACATATCAGATTTTGCCACTGGTGTTTGCTTCGCTTCTGCAGGAACTGGCTATGATAATGCTACTTCTGATGTTGTACAAGTGATTCCACTGTGGAAAGAAGTGGAGTACTACAAGGAATACCAAAAGAAACTAAAAGCCTATCTTGGAGTTAGGAATGCAAAGAAGCTACTAAGCGAGGCATTATATTTGATCAGCATAGGAACAAATGACTTCCTTGAAAACTATTTTACACTCCCGAACCGGCAGTCCCAGTTCACCGTGAAACAGTACGAAGATTTCCTGATCGATCTTGCCGCAGATTTTCTGAAGAAACTATACGTTCTAGGAGCTCGGAAGATATCGCTCGCGGGGCTTCCTCCAATGGGGTGTTTGCCATTGGAGAGGACCACAAATATTATGGGGCAAAATTCATGTGTGGAGGAACGCAACAACTTAGCTTTGGAGTTTAATGGCAAGTTGAAGGGTTTGGTGGCAAAGCTGAATGAGGAGCTTCCGGGGCTTGATTTGATGTTTTCAGATGTTTATTACATTTTCTTGCAAATCATAAAAAGGCCTTCTGCATTTGGATTGGAGGAAGCACGAGTGGGATGCTGCGGCACAGGAACATTTGAGATGAGTTTCCTCTGCGATCAGTTCAATCCATTTACTTGTCAGGATGCAAATAAATACGTGTTTTGGGATGCCTTCCATCCTTCTGAGAAAACCAATCGAATCATCTCAGAATATGCACTCAAAACTTCTCTAGCAAAGTTCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

350

Amino Acids

39.05

Weight (kDa)

6.35

Isoelectric Point (pI)

30.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 28 - 339 9e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013813)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42990
fragaria_vesca FvH4_6g38660 FvH4_6g38660
malus_domestica MD09G1148500.v1.1
prunus_persica Prupe.3G177600_v2.0.a1
pyrus_communis pycom09g06820
rosa_chinensis RchiOBHm_Chr2g0152121
rosa_laevigata RLG00000020610
rosa_multiflora Rmu_sc0000760.1_g000001 Rmu_sc0003043.1_g000023
rosa_roxburghii Rroxscaffold_2G00095910
rosa_rugosa Rorug02G0431200
rosa_samantha Rh2AG492000 Rh2BG504500 Rh2CG478500 Rh2DG515800
rosa_wichuraiana Rw2G040440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 274
AccII CGCG 1 cut(s) 638
AciI CCGC 4 cut(s) 199, 585, 638, 885
AclWI GGATC 1 cut(s) 272
AcoI YGGCCR 1 cut(s) 211
AcsI RAATTY 1 cut(s) 697
AcuI CTGAAG 1 cut(s) 617
AfaI GTAC 3 cut(s) 366, 398, 560
AfiI CCNNNNNNNGG 2 cut(s) 198, 653
AflIII ACRYGT 1 cut(s) 960
AgsI TTSAA 3 cut(s) 509, 751, 928
AjnI CCWGG 2 cut(s) 20, 143
AloI GAACNNNNNNTCC 2 cut(s) 521, 553
AluBI AGCT 6 cut(s) 68, 457, 620, 728, 769, 781
AluI AGCT 6 cut(s) 68, 457, 620, 728, 769, 781
Alw21I GWGCWC 1 cut(s) 622
AlwI GGATC 1 cut(s) 272
AlwNI CAGNNNCTG 1 cut(s) 335
AoxI GGCC 3 cut(s) 184, 211, 842
ApeKI GCWGC 2 cut(s) 249, 882
ApoI RAATTY 1 cut(s) 697
ArsI GACNNNNNNTTYG 2 cut(s) 521, 553
AspS9I GGNCC 1 cut(s) 675
AsuC2I CCSGG 3 cut(s) 180, 196, 786
AsuHPI GGTGA 1 cut(s) 538
AvaII GGWCC 1 cut(s) 675
BanII GRGCYC 1 cut(s) 622
BarI GAAGNNNNNNTAC 2 cut(s) 590, 622
BauI CACGAG 1 cut(s) 870
Bbv12I GWGCWC 1 cut(s) 622
BbvI GCAGC 2 cut(s) 236, 869
BccI CCATC 1 cut(s) 987
BcgI CGANNNNNNTGC 2 cut(s) 861, 895
BciT130I CCWGG 2 cut(s) 22, 145
BclI TGATCA 1 cut(s) 480
BcnI CCSGG 3 cut(s) 180, 196, 786
BfaI CTAG 3 cut(s) 245, 614, 1034
BfmI CTRYAG 1 cut(s) 327
BfuAI ACCTGC 1 cut(s) 274
BglI GCCNNNNNGGC 1 cut(s) 196
BisI GCNGC 4 cut(s) 250, 585, 883, 886
BlsI GCNGC 4 cut(s) 251, 586, 884, 887
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 5 cut(s) 22, 145, 180, 196, 786
Bme18I GGWCC 1 cut(s) 675
BmgT120I GGNCC 1 cut(s) 675
BmrFI CCNGG 5 cut(s) 22, 145, 180, 196, 786
BmrI ACTGGG 1 cut(s) 535
BmsI GCATC 3 cut(s) 869, 937, 961
BmuI ACTGGG 1 cut(s) 535
BpuMI CCSGG 3 cut(s) 180, 196, 786
Bsa29I ATCGAT 1 cut(s) 576
BsaAI YACGTR 1 cut(s) 961
BsaJI CCNNGG 2 cut(s) 20, 785
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 2 cut(s) 198, 653
Bse118I RCCGGY 1 cut(s) 531
Bse1I ACTGG 3 cut(s) 313, 340, 541
Bse3DI GCAATG 1 cut(s) 214
BseBI CCWGG 2 cut(s) 22, 145
BseCI ATCGAT 1 cut(s) 576
BseDI CCNNGG 2 cut(s) 20, 785
BseGI GGATG 4 cut(s) 884, 952, 976, 979
BseLI CCNNNNNNNGG 2 cut(s) 198, 653
BseMI GCAATG 1 cut(s) 214
BseMII CTCAG 2 cut(s) 978, 1023
BseNI ACTGG 3 cut(s) 313, 340, 541
BseRI GAGGAG 1 cut(s) 791
BseXI GCAGC 2 cut(s) 236, 869
Bsh1236I CGCG 1 cut(s) 638
BshFI GGCC 3 cut(s) 186, 213, 844
BshVI ATCGAT 1 cut(s) 576
BsiHKAI GWGCWC 1 cut(s) 622
BsiSI CCGG 5 cut(s) 179, 195, 214, 532, 785
BslFI GGGAC 1 cut(s) 523
BslI CCNNNNNNNGG 2 cut(s) 198, 653
BsmFI GGGAC 1 cut(s) 523
BsmI GAATGC 1 cut(s) 451
BsnI GGCC 3 cut(s) 186, 213, 844
Bsp1286I GDGCHC 1 cut(s) 622
Bsp1407I TGTACA 1 cut(s) 364
Bsp143I GATC 5 cut(s) 277, 480, 573, 577, 919
BspACI CCGC 4 cut(s) 199, 585, 638, 885
BspANI GGCC 3 cut(s) 186, 213, 844
BspCNI CTCAG 2 cut(s) 979, 1022
BspDI ATCGAT 1 cut(s) 576
BspFNI CGCG 1 cut(s) 638
BspMAI CTGCAG 1 cut(s) 331
BspMI ACCTGC 1 cut(s) 274
BspPI GGATC 1 cut(s) 272
BsrDI GCAATG 1 cut(s) 214
BsrFI RCCGGY 1 cut(s) 531
BsrGI TGTACA 1 cut(s) 364
BsrI ACTGG 3 cut(s) 313, 340, 541
BssAI RCCGGY 1 cut(s) 531
BssECI CCNNGG 2 cut(s) 20, 785
BssMI GATC 5 cut(s) 277, 480, 573, 577, 919
BssSI CACGAG 1 cut(s) 870
Bst2BI CACGAG 1 cut(s) 870
Bst2UI CCWGG 2 cut(s) 22, 145
Bst4CI ACNGT 3 cut(s) 381, 550, 558
BstAUI TGTACA 1 cut(s) 364
BstBAI YACGTR 1 cut(s) 961
BstC8I GCNNGC 3 cut(s) 188, 254, 636
BstDEI CTNAG 6 cut(s) 31, 274, 461, 724, 987, 1009
BstF5I GGATG 4 cut(s) 884, 952, 976, 979
BstFNI CGCG 1 cut(s) 638
BstKTI GATC 5 cut(s) 280, 483, 576, 580, 922
BstMBI GATC 5 cut(s) 277, 480, 573, 577, 919
BstMWI GCNNNNNNNGC 3 cut(s) 196, 640, 787
BstNI CCWGG 2 cut(s) 22, 145
BstSCI CCNGG 5 cut(s) 20, 143, 178, 194, 784
BstSFI CTRYAG 1 cut(s) 327
BstUI CGCG 1 cut(s) 638
BstV1I GCAGC 2 cut(s) 236, 869
BstX2I RGATCY 1 cut(s) 277
BstXI CCANNNNNNTGG 1 cut(s) 164
BstYI RGATCY 1 cut(s) 277
Bsu15I ATCGAT 1 cut(s) 576
BsuRI GGCC 3 cut(s) 186, 213, 844
BsuTUI ATCGAT 1 cut(s) 576
BtsCI GGATG 4 cut(s) 884, 952, 976, 979
BtsIMutI CAGTG 2 cut(s) 306, 377
BveI ACCTGC 1 cut(s) 274
Cac8I GCNNGC 3 cut(s) 188, 254, 636
CaiI CAGNNNCTG 1 cut(s) 335
Cfr10I RCCGGY 1 cut(s) 531
Cfr13I GGNCC 1 cut(s) 675
ClaI ATCGAT 1 cut(s) 576
Csp6I GTAC 3 cut(s) 365, 397, 559
CviAII CATG 1 cut(s) 702
CviQI GTAC 3 cut(s) 365, 397, 559
DdeI CTNAG 6 cut(s) 31, 274, 461, 724, 987, 1009
DpnI GATC 5 cut(s) 279, 482, 575, 579, 921
DpnII GATC 5 cut(s) 277, 480, 573, 577, 919
EaeI YGGCCR 1 cut(s) 211
Ecl136II GAGCTC 1 cut(s) 620
Eco147I AGGCCT 1 cut(s) 844
Eco24I GRGCYC 1 cut(s) 622
Eco32I GATATC 1 cut(s) 630
Eco47I GGWCC 1 cut(s) 675
Eco53kI GAGCTC 1 cut(s) 620
Eco57I CTGAAG 1 cut(s) 617
EcoICRI GAGCTC 1 cut(s) 620
EcoRII CCWGG 2 cut(s) 20, 143
EcoRV GATATC 1 cut(s) 630
EcoT38I GRGCYC 1 cut(s) 622
FaeI CATG 1 cut(s) 705
FalI AAGNNNNNCTT 2 cut(s) 417, 449
FaqI GGGAC 1 cut(s) 523
FatI CATG 1 cut(s) 701
FauI CCCGC 1 cut(s) 631
FbaI TGATCA 1 cut(s) 480
Fnu4HI GCNGC 4 cut(s) 250, 585, 883, 886
FokI GGATG 4 cut(s) 891, 959, 966, 983
FriOI GRGCYC 1 cut(s) 622
Fsp4HI GCNGC 4 cut(s) 250, 585, 883, 886
FspBI CTAG 3 cut(s) 245, 614, 1034
GluI GCNGC 4 cut(s) 250, 585, 883, 886
HaeIII GGCC 3 cut(s) 186, 213, 844
HapII CCGG 5 cut(s) 179, 195, 214, 532, 785
Hin1II CATG 1 cut(s) 705
HinfI GANTC 5 cut(s) 98, 110, 217, 373, 1002
HpaII CCGG 5 cut(s) 179, 195, 214, 532, 785
HphI GGTGA 1 cut(s) 538
Hpy166II GTNNAC 1 cut(s) 546
Hpy188I TCNGA 8 cut(s) 238, 298, 358, 597, 624, 808, 988, 1012
Hpy188III TCNNGA 5 cut(s) 170, 224, 526, 571, 944
Hpy8I GTNNAC 1 cut(s) 546
HpyAV CCTTC 5 cut(s) 233, 745, 855, 985, 993
HpyCH4III ACNGT 3 cut(s) 381, 550, 558
HpyCH4IV ACGT 2 cut(s) 609, 960
HpyCH4V TGCA 8 cut(s) 207, 252, 329, 449, 829, 851, 950, 1019
HpyF10VI GCNNNNNNNGC 3 cut(s) 196, 640, 787
HpyF3I CTNAG 6 cut(s) 31, 274, 461, 724, 987, 1009
HpySE526I ACGT 2 cut(s) 609, 960
Hsp92II CATG 1 cut(s) 705
Ksp22I TGATCA 1 cut(s) 480
Kzo9I GATC 5 cut(s) 277, 480, 573, 577, 919
LmnI GCTCC 3 cut(s) 147, 617, 778
Lsp1109I GCAGC 2 cut(s) 236, 869
LweI GCATC 3 cut(s) 869, 937, 961
MaeI CTAG 3 cut(s) 245, 614, 1034
MaeII ACGT 2 cut(s) 609, 960
MaeIII GTNAC 1 cut(s) 116
MalI GATC 5 cut(s) 279, 482, 575, 579, 921
MboI GATC 5 cut(s) 277, 480, 573, 577, 919
MboII GAAGA 4 cut(s) 93, 575, 610, 637
MfeI CAATTG 1 cut(s) 138
MflI RGATCY 1 cut(s) 277
MhlI GDGCHC 1 cut(s) 622
MluCI AATT 3 cut(s) 138, 258, 697
MmeI TCCRAC 1 cut(s) 305
MnlI CCTC 9 cut(s) 176, 231, 460, 657, 666, 703, 769, 856, 923
MseI TTAA 2 cut(s) 738, 1048
MspI CCGG 5 cut(s) 179, 195, 214, 532, 785
MspR9I CCNGG 5 cut(s) 22, 145, 180, 196, 786
MunI CAATTG 1 cut(s) 138
Mva1269I GAATGC 1 cut(s) 451
MvaI CCWGG 2 cut(s) 22, 145
MvnI CGCG 1 cut(s) 638
MwoI GCNNNNNNNGC 3 cut(s) 196, 640, 787
NciI CCSGG 3 cut(s) 180, 196, 786
NdeII GATC 5 cut(s) 277, 480, 573, 577, 919
NlaIII CATG 1 cut(s) 705
PceI AGGCCT 1 cut(s) 844
PctI GAATGC 1 cut(s) 451
PfeI GAWTC 5 cut(s) 98, 110, 217, 373, 1002
PfoI TCCNGGA 1 cut(s) 178
PkrI GCNGC 4 cut(s) 251, 586, 884, 887
Ppu21I YACGTR 1 cut(s) 961
Psp124BI GAGCTC 1 cut(s) 622
Psp6I CCWGG 2 cut(s) 20, 143
PspGI CCWGG 2 cut(s) 20, 143
PspPI GGNCC 1 cut(s) 675
PstI CTGCAG 1 cut(s) 331
PstNI CAGNNNCTG 1 cut(s) 335
PsuI RGATCY 1 cut(s) 277
RsaI GTAC 3 cut(s) 366, 398, 560
RsaNI GTAC 3 cut(s) 365, 397, 559
SacI GAGCTC 1 cut(s) 622
SaqAI TTAA 2 cut(s) 738, 1048
SatI GCNGC 4 cut(s) 250, 585, 883, 886
Sau3AI GATC 5 cut(s) 277, 480, 573, 577, 919
Sau96I GGNCC 1 cut(s) 675
ScaI AGTACT 1 cut(s) 398
ScrFI CCNGG 5 cut(s) 22, 145, 180, 196, 786
SduI GDGCHC 1 cut(s) 622
SfaNI GCATC 3 cut(s) 869, 937, 961
SfcI CTRYAG 1 cut(s) 327
SinI GGWCC 1 cut(s) 675
Sse9I AATT 3 cut(s) 138, 258, 697
SseBI AGGCCT 1 cut(s) 844
SsiI CCGC 4 cut(s) 199, 585, 638, 885
SspI AATATT 1 cut(s) 685
SspMI CTAG 3 cut(s) 245, 614, 1034
SstI GAGCTC 1 cut(s) 622
StuI AGGCCT 1 cut(s) 844
StyD4I CCNGG 5 cut(s) 20, 143, 178, 194, 784
TaaI ACNGT 3 cut(s) 381, 550, 558
TaiI ACGT 2 cut(s) 612, 963
TaqI TCGA 2 cut(s) 576, 1000
TasI AATT 3 cut(s) 138, 258, 697
TatI WGTACW 2 cut(s) 364, 396
TauI GCSGC 2 cut(s) 587, 888
TfiI GAWTC 5 cut(s) 98, 110, 217, 373, 1002
Tru1I TTAA 2 cut(s) 738, 1048
Tru9I TTAA 2 cut(s) 738, 1048
TscAI CASTG 2 cut(s) 313, 384
TseI GCWGC 2 cut(s) 249, 882
TspDTI ATGAA 4 cut(s) 26, 118, 220, 690
TspRI CASTG 2 cut(s) 313, 384
VpaK11BI GGWCC 1 cut(s) 675
XapI RAATTY 1 cut(s) 697
XcmI CCANNNNNNNNNTGG 1 cut(s) 685
XspI CTAG 3 cut(s) 245, 614, 1034
ZrmI AGTACT 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.