Prupe.3G177600_v2.0.a1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0013813
Physical Location & Seq
Forward (+)
19446613 .. 19448920
2308 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G177600.1

Sequence Viewer

Length: 1050 bp
ATGGCAAACTTGTACATTCCTTGGATTTTCTTAGTTCAAACACTAACACTATTTGCCAAATGCAGAGGTAAAGTTCCAGCAGTCATAGTGTTTGGAGATTCTTCTGTTGACTCAGGTAACAACAACTTCATTCCAACAATTGCCAGGAGCAACTTTCAGCCTTATGGTCAAGATTTTCCGGGAGGCCAACCCACGGGGCGGTTCTGCAACGGCCGAATTCCTTCTGACCTCATCTCTGATGGTCTAGGCCTTAAGCCAACCATACCTGCCTACTTGGATCCAATTTATAACATATCAGATTTTGCTACTGGTGTTTGCTTTGCTTCTGCAGGAACCGGCTATGATAACACAACTTCTGATGTTGCTCGTGTGATTCCACTGTGGAAGGAAGTAGAATACTACGAGGAATACCAGAAGAAATTAAAAGCCTATCTTGGAAATAAGAAGGCAAAAAAATTACTAAGTGATGCTTTATATTTGATTAGCATAGGAACAAATGACTTCCTTGAAAACTACTATACATTACCGGACCGACAATCCCAGTTCACCGTGAAGCAGTACGAGAACTTCCTGATCAATCTTGCAGCAGATTTTGTGAAGAAACTATATGCTCTTGGGGCTCGGAGGATGTCCCTAGCAGGGCTTCCTCCAATGGGGTGTTTGCCATTGGAAAGGACCACAAATATCATGGGACTGCATGCATGTGTGGAGGAACGCAACAATGTGGCTTTGGAGTTTAATCGGAAGTTGAAGGGTATGGTGGCAAAGCTGAACAAGCAGCTTCCGGGGCTTGAGGCGGTATATGGAGATGTTTATTACATTTTCTTGCAAATCATAACAAGGCCTTCTGTTTTTGGATTGGAGGAAGCAAGAGTGGGATGCTGTGGCACGGGGAGATTTGAGATGAGTTTCCTATGTAATCCACATAATCCCTTTACGTGCCAAGACGCAAATAAGTACGTATTTTGGGATGCCTTCCATCCTTCAGAGAAAACCAATCAAATCATCTCAGATAATGCTCTTAAAACTTATTTAGCAAAGTTTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

350

Amino Acids

39.11

Weight (kDa)

8.44

Isoelectric Point (pI)

30.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013813)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42990
fragaria_vesca FvH4_6g38660 FvH4_6g38660
malus_domestica MD09G1148500.v1.1
prunus_persica Prupe.3G177600_v2.0.a1
pyrus_communis pycom09g06820
rosa_chinensis RchiOBHm_Chr2g0152121
rosa_laevigata RLG00000020610
rosa_multiflora Rmu_sc0000760.1_g000001 Rmu_sc0003043.1_g000023
rosa_roxburghii Rroxscaffold_2G00095910
rosa_rugosa Rorug02G0431200
rosa_samantha Rh2AG492000 Rh2BG504500 Rh2CG478500 Rh2DG515800
rosa_wichuraiana Rw2G040440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 288
Acc36I ACCTGC 1 cut(s) 274
AciI CCGC 2 cut(s) 199, 797
AclWI GGATC 2 cut(s) 272, 285
AcoI YGGCCR 1 cut(s) 211
AcsI RAATTY 1 cut(s) 216
AcuI CTGAAG 1 cut(s) 969
AfaI GTAC 3 cut(s) 14, 560, 959
AfiI CCNNNNNNNGG 4 cut(s) 193, 198, 639, 653
AflII CTTAAG 1 cut(s) 251
AgsI TTSAA 3 cut(s) 38, 509, 751
AjnI CCWGG 1 cut(s) 143
AluBI AGCT 2 cut(s) 769, 781
AluI AGCT 2 cut(s) 769, 781
AlwI GGATC 2 cut(s) 272, 285
AoxI GGCC 4 cut(s) 184, 211, 247, 842
ApeKI GCWGC 2 cut(s) 584, 778
ApoI RAATTY 1 cut(s) 216
Asp700I GAANNNNTTC 1 cut(s) 220
AspS9I GGNCC 2 cut(s) 529, 675
AsuC2I CCSGG 2 cut(s) 180, 786
AsuHPI GGTGA 1 cut(s) 538
AvaII GGWCC 2 cut(s) 529, 675
BamHI GGATCC 1 cut(s) 277
BanII GRGCYC 1 cut(s) 622
BarI GAAGNNNNNNTAC 2 cut(s) 551, 583
BauI CACGAG 1 cut(s) 366
BbvI GCAGC 2 cut(s) 596, 790
BccI CCATC 2 cut(s) 233, 987
BceAI ACGGC 1 cut(s) 226
BciT130I CCWGG 1 cut(s) 145
BclI TGATCA 1 cut(s) 573
BcnI CCSGG 2 cut(s) 180, 786
BfaI CTAG 2 cut(s) 245, 635
BfmI CTRYAG 1 cut(s) 327
BfrI CTTAAG 1 cut(s) 251
BfuAI ACCTGC 1 cut(s) 274
BisI GCNGC 2 cut(s) 585, 779
BlsI GCNGC 2 cut(s) 586, 780
Bme1390I CCNGG 3 cut(s) 145, 180, 786
Bme18I GGWCC 2 cut(s) 529, 675
BmgT120I GGNCC 2 cut(s) 529, 675
BmiI GGNNCC 2 cut(s) 279, 334
BmrFI CCNGG 3 cut(s) 145, 180, 786
BmrI ACTGGG 1 cut(s) 535
BmsI GCATC 3 cut(s) 457, 869, 961
BmuI ACTGGG 1 cut(s) 535
BpuEI CTTGAG 1 cut(s) 812
BpuMI CCSGG 2 cut(s) 180, 786
BsaAI YACGTR 2 cut(s) 939, 961
BsaJI CCNNGG 3 cut(s) 20, 192, 785
BsaWI WCCGGW 1 cut(s) 526
Bsc4I CCNNNNNNNGG 4 cut(s) 193, 198, 639, 653
Bse118I RCCGGY 1 cut(s) 335
Bse1I ACTGG 2 cut(s) 313, 541
BseBI CCWGG 1 cut(s) 145
BseDI CCNNGG 3 cut(s) 20, 192, 785
BseGI GGATG 4 cut(s) 633, 884, 976, 979
BseLI CCNNNNNNNGG 4 cut(s) 193, 198, 639, 653
BseMII CTCAG 2 cut(s) 126, 1023
BseNI ACTGG 2 cut(s) 313, 541
BseX3I CGGCCG 1 cut(s) 211
BseXI GCAGC 2 cut(s) 596, 790
Bsh1285I CGRYCG 1 cut(s) 214
BshFI GGCC 4 cut(s) 186, 213, 249, 844
BsiEI CGRYCG 1 cut(s) 214
BsiSI CCGG 4 cut(s) 179, 336, 527, 785
BslFI GGGAC 2 cut(s) 616, 705
BslI CCNNNNNNNGG 4 cut(s) 193, 198, 639, 653
BsmFI GGGAC 2 cut(s) 616, 705
BsnI GGCC 4 cut(s) 186, 213, 249, 844
Bsp1286I GDGCHC 1 cut(s) 622
Bsp1407I TGTACA 1 cut(s) 12
Bsp143I GATC 2 cut(s) 277, 573
BspACI CCGC 2 cut(s) 199, 797
BspANI GGCC 4 cut(s) 186, 213, 249, 844
BspCNI CTCAG 2 cut(s) 125, 1022
BspLI GGNNCC 2 cut(s) 279, 334
BspMAI CTGCAG 1 cut(s) 331
BspMI ACCTGC 1 cut(s) 274
BspPI GGATC 2 cut(s) 272, 285
BspTI CTTAAG 1 cut(s) 251
BsrFI RCCGGY 1 cut(s) 335
BsrGI TGTACA 1 cut(s) 12
BsrI ACTGG 2 cut(s) 313, 541
BssAI RCCGGY 1 cut(s) 335
BssECI CCNNGG 3 cut(s) 20, 192, 785
BssMI GATC 2 cut(s) 277, 573
BssSI CACGAG 1 cut(s) 366
BssT1I CCWWGG 1 cut(s) 20
Bst2BI CACGAG 1 cut(s) 366
Bst2UI CCWGG 1 cut(s) 145
Bst4CI ACNGT 2 cut(s) 381, 550
BstAFI CTTAAG 1 cut(s) 251
BstAUI TGTACA 1 cut(s) 12
BstBAI YACGTR 2 cut(s) 939, 961
BstC8I GCNNGC 1 cut(s) 699
BstDEI CTNAG 4 cut(s) 31, 112, 461, 1009
BstDSI CCRYGG 1 cut(s) 192
BstF5I GGATG 4 cut(s) 633, 884, 976, 979
BstKTI GATC 2 cut(s) 280, 576
BstMBI GATC 2 cut(s) 277, 573
BstMCI CGRYCG 1 cut(s) 214
BstMWI GCNNNNNNNGC 3 cut(s) 617, 775, 787
BstNI CCWGG 1 cut(s) 145
BstNSI RCATGY 2 cut(s) 701, 705
BstSCI CCNGG 3 cut(s) 143, 178, 784
BstSFI CTRYAG 1 cut(s) 327
BstSNI TACGTA 1 cut(s) 961
BstV1I GCAGC 2 cut(s) 596, 790
BstX2I RGATCY 1 cut(s) 277
BstYI RGATCY 1 cut(s) 277
BstZI CGGCCG 1 cut(s) 211
BsuRI GGCC 4 cut(s) 186, 213, 249, 844
BtgI CCRYGG 1 cut(s) 192
BtsCI GGATG 4 cut(s) 633, 884, 976, 979
BtsIMutI CAGTG 1 cut(s) 377
BveI ACCTGC 1 cut(s) 274
Cac8I GCNNGC 1 cut(s) 699
Cfr10I RCCGGY 1 cut(s) 335
Cfr13I GGNCC 2 cut(s) 529, 675
CpoI CGGWCCG 1 cut(s) 529
CseI GACGC 1 cut(s) 956
Csp6I GTAC 3 cut(s) 13, 559, 958
CspI CGGWCCG 1 cut(s) 529
CviAII CATG 3 cut(s) 688, 698, 702
CviQI GTAC 3 cut(s) 13, 559, 958
DdeI CTNAG 4 cut(s) 31, 112, 461, 1009
DpnI GATC 2 cut(s) 279, 575
DpnII GATC 2 cut(s) 277, 573
EaeI YGGCCR 1 cut(s) 211
EagI CGGCCG 1 cut(s) 211
EclXI CGGCCG 1 cut(s) 211
Eco105I TACGTA 1 cut(s) 961
Eco130I CCWWGG 1 cut(s) 20
Eco147I AGGCCT 2 cut(s) 249, 844
Eco24I GRGCYC 1 cut(s) 622
Eco47I GGWCC 2 cut(s) 529, 675
Eco52I CGGCCG 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 969
EcoRI GAATTC 1 cut(s) 216
EcoRII CCWGG 1 cut(s) 143
EcoT14I CCWWGG 1 cut(s) 20
EcoT22I ATGCAT 1 cut(s) 703
EcoT38I GRGCYC 1 cut(s) 622
ErhI CCWWGG 1 cut(s) 20
FaeI CATG 3 cut(s) 691, 701, 705
FalI AAGNNNNNCTT 4 cut(s) 417, 449, 454, 486
FaqI GGGAC 2 cut(s) 616, 705
FatI CATG 3 cut(s) 687, 697, 701
FbaI TGATCA 1 cut(s) 573
Fnu4HI GCNGC 2 cut(s) 585, 779
FokI GGATG 4 cut(s) 640, 891, 966, 983
FriOI GRGCYC 1 cut(s) 622
Fsp4HI GCNGC 2 cut(s) 585, 779
FspBI CTAG 2 cut(s) 245, 635
GluI GCNGC 2 cut(s) 585, 779
HaeIII GGCC 4 cut(s) 186, 213, 249, 844
HapII CCGG 4 cut(s) 179, 336, 527, 785
HgaI GACGC 1 cut(s) 956
Hin1II CATG 3 cut(s) 691, 701, 705
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HinfI GANTC 3 cut(s) 98, 110, 373
HpaII CCGG 4 cut(s) 179, 336, 527, 785
HphI GGTGA 1 cut(s) 538
Hpy166II GTNNAC 2 cut(s) 109, 546
Hpy188I TCNGA 8 cut(s) 226, 238, 298, 358, 624, 744, 988, 1012
Hpy188III TCNNGA 2 cut(s) 170, 571
Hpy8I GTNNAC 2 cut(s) 109, 546
HpyAV CCTTC 7 cut(s) 231, 379, 439, 745, 855, 985, 993
HpyCH4III ACNGT 2 cut(s) 381, 550
HpyCH4IV ACGT 2 cut(s) 938, 960
HpyCH4V TGCA 7 cut(s) 63, 207, 329, 584, 697, 701, 829
HpyF10VI GCNNNNNNNGC 3 cut(s) 617, 775, 787
HpyF3I CTNAG 4 cut(s) 31, 112, 461, 1009
HpySE526I ACGT 2 cut(s) 938, 960
Hsp92II CATG 3 cut(s) 691, 701, 705
Ksp22I TGATCA 1 cut(s) 573
Kzo9I GATC 2 cut(s) 277, 573
LmnI GCTCC 1 cut(s) 147
Lsp1109I GCAGC 2 cut(s) 596, 790
LweI GCATC 3 cut(s) 457, 869, 961
MaeI CTAG 2 cut(s) 245, 635
MaeII ACGT 2 cut(s) 938, 960
MaeIII GTNAC 1 cut(s) 116
MalI GATC 2 cut(s) 279, 575
MboI GATC 2 cut(s) 277, 573
MboII GAAGA 3 cut(s) 93, 427, 610
MfeI CAATTG 1 cut(s) 138
MflI RGATCY 1 cut(s) 277
MhlI GDGCHC 1 cut(s) 622
MluCI AATT 5 cut(s) 138, 216, 282, 419, 455
MlyI GAGTC 1 cut(s) 104
MmeI TCCRAC 1 cut(s) 158
MnlI CCTC 9 cut(s) 59, 176, 239, 397, 618, 657, 703, 787, 856
Mph1103I ATGCAT 1 cut(s) 703
MroXI GAANNNNTTC 1 cut(s) 220
MseI TTAA 4 cut(s) 252, 422, 738, 1023
MslI CAYNNNNRTG 1 cut(s) 702
MspCI CTTAAG 1 cut(s) 251
MspI CCGG 4 cut(s) 179, 336, 527, 785
MspR9I CCNGG 3 cut(s) 145, 180, 786
MunI CAATTG 1 cut(s) 138
MvaI CCWGG 1 cut(s) 145
MwoI GCNNNNNNNGC 3 cut(s) 617, 775, 787
NciI CCSGG 2 cut(s) 180, 786
NdeII GATC 2 cut(s) 277, 573
NlaIII CATG 3 cut(s) 691, 701, 705
NlaIV GGNNCC 2 cut(s) 279, 334
NsiI ATGCAT 1 cut(s) 703
NspI RCATGY 2 cut(s) 701, 705
PaeI GCATGC 1 cut(s) 701
PceI AGGCCT 2 cut(s) 249, 844
PdmI GAANNNNTTC 1 cut(s) 220
PfeI GAWTC 2 cut(s) 98, 373
PfoI TCCNGGA 1 cut(s) 178
PkrI GCNGC 2 cut(s) 586, 780
PleI GAGTC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 104
Ppu21I YACGTR 2 cut(s) 939, 961
PsiI TTATAA 1 cut(s) 288
Psp6I CCWGG 1 cut(s) 143
PspGI CCWGG 1 cut(s) 143
PspN4I GGNNCC 2 cut(s) 279, 334
PspPI GGNCC 2 cut(s) 529, 675
PstI CTGCAG 1 cut(s) 331
PsuI RGATCY 1 cut(s) 277
RsaI GTAC 3 cut(s) 14, 560, 959
RsaNI GTAC 3 cut(s) 13, 559, 958
RseI CAYNNNNRTG 1 cut(s) 702
Rsr2I CGGWCCG 1 cut(s) 529
RsrII CGGWCCG 1 cut(s) 529
SaqAI TTAA 4 cut(s) 252, 422, 738, 1023
SatI GCNGC 2 cut(s) 585, 779
Sau3AI GATC 2 cut(s) 277, 573
Sau96I GGNCC 2 cut(s) 529, 675
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 3 cut(s) 145, 180, 786
SduI GDGCHC 1 cut(s) 622
SetI ASST 8 cut(s) 70, 118, 231, 268, 771, 783, 941, 963
SfaNI GCATC 3 cut(s) 457, 869, 961
SfcI CTRYAG 1 cut(s) 327
SinI GGWCC 2 cut(s) 529, 675
SmiMI CAYNNNNRTG 1 cut(s) 702
SmlI CTYRAG 2 cut(s) 251, 791
SmoI CTYRAG 2 cut(s) 251, 791
SnaBI TACGTA 1 cut(s) 961
SphI GCATGC 1 cut(s) 701
Sse9I AATT 5 cut(s) 138, 216, 282, 419, 455
SseBI AGGCCT 2 cut(s) 249, 844
SsiI CCGC 2 cut(s) 199, 797
SspMI CTAG 2 cut(s) 245, 635
StuI AGGCCT 2 cut(s) 249, 844
StyD4I CCNGG 3 cut(s) 143, 178, 784
StyI CCWWGG 1 cut(s) 20
TaaI ACNGT 2 cut(s) 381, 550
TaiI ACGT 2 cut(s) 941, 963
TaqII GACCGA 1 cut(s) 546
TasI AATT 5 cut(s) 138, 216, 282, 419, 455
TatI WGTACW 1 cut(s) 12
TfiI GAWTC 2 cut(s) 98, 373
Tru1I TTAA 4 cut(s) 252, 422, 738, 1023
Tru9I TTAA 4 cut(s) 252, 422, 738, 1023
TscAI CASTG 1 cut(s) 384
TseI GCWGC 2 cut(s) 584, 778
TspDTI ATGAA 1 cut(s) 118
TspRI CASTG 1 cut(s) 384
Vha464I CTTAAG 1 cut(s) 251
VpaK11BI GGWCC 2 cut(s) 529, 675
XapI RAATTY 1 cut(s) 216
XceI RCATGY 2 cut(s) 701, 705
XcmI CCANNNNNNNNNTGG 1 cut(s) 685
XmnI GAANNNNTTC 1 cut(s) 220
XspI CTAG 2 cut(s) 245, 635
Zsp2I ATGCAT 1 cut(s) 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.