Rh2DG515800

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0013813
Physical Location & Seq
Forward (+)
73947884 .. 73949054
1171 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG515800.1

Sequence Viewer

Length: 768 bp
ATGTATAGCATCTCAGATTTTGCTGTAGGAGTTTGCTTTGCTTCTGCAGGGACTGGCTATGATAATGCCACTTCTGAAGTTGCTGATGTGATTCCATTGTGGAAGGAAGTGGAGTATTACAAGGAGTACCAGCAGAAATTAAAAGCCTACCTTGGAGATAGGAAGGCAAAGAAAATACTAAGTGAGGCTTTGTATTTGATTAGCTTAGGAACAAATGACTTCATGGAAAACTATTTTACACTCCCAAACCGACGATTGCAGTTTACCGTGAACCAATATCAGGATTTTATTATAGGACTTGCTGCAGATTTTGTGAAGACAATATATTCTCTAGGGGCAAGGAAGATGTCCCTGACAGGGGTTCCTCCAATGGGGTGTTTGCCACTGGAAAGAACCACAAATATTATGGAAGACCATGCTTGCATGGAGGAATACAACAACGTTGGTTTGGAATTTAATGGCAAGTTAAAGGGTTTGGTGGCAAAGCTGAACAGCGAGCTTCCTGGTCTTGATGTGGTATTTGCAGATGCATATAACCTCTTGTTGCAACTCATAAAAAGGCCTTCTGTTTATGGATTTGAGGAATCAAGAACTGGGTGCTGCGGCACAGGGAGGTTTGAGATGAGTTTCCTATGTGATCCGCATAGTCCATTTACATGCCAAGATGCAGATAAGTATGTGTTTTGGGATGCCTTTCATCCTTCGGAGAAAACCAATCGAATGATCACAGATCATCTACTCAAAACTTCTTTAGCAAAGTTTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

255

Amino Acids

28.84

Weight (kDa)

5.47

Isoelectric Point (pI)

34.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 8 - 245 1.6e-27 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013813)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42990
fragaria_vesca FvH4_6g38660 FvH4_6g38660
malus_domestica MD09G1148500.v1.1
prunus_persica Prupe.3G177600_v2.0.a1
pyrus_communis pycom09g06820
rosa_chinensis RchiOBHm_Chr2g0152121
rosa_laevigata RLG00000020610
rosa_multiflora Rmu_sc0000760.1_g000001 Rmu_sc0003043.1_g000023
rosa_roxburghii Rroxscaffold_2G00095910
rosa_rugosa Rorug02G0431200
rosa_samantha Rh2AG492000 Rh2BG504500 Rh2CG478500 Rh2DG515800
rosa_wichuraiana Rw2G040440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 603, 641
AclI AACGTT 1 cut(s) 441
AclWI GGATC 1 cut(s) 632
AcsI RAATTY 1 cut(s) 452
AcuI CTGAAG 1 cut(s) 96
AfaI GTAC 1 cut(s) 128
AfiI CCNNNNNNNGG 4 cut(s) 280, 357, 358, 371
AjnI CCWGG 1 cut(s) 502
AluBI AGCT 3 cut(s) 204, 487, 499
AluI AGCT 3 cut(s) 204, 487, 499
AlwI GGATC 1 cut(s) 632
AlwNI CAGNNNCTG 1 cut(s) 53
AoxI GGCC 1 cut(s) 560
ApeKI GCWGC 2 cut(s) 302, 600
ApoI RAATTY 1 cut(s) 452
BbsI GAAGAC 2 cut(s) 323, 417
BbvI GCAGC 2 cut(s) 289, 587
BciT130I CCWGG 1 cut(s) 504
BclI TGATCA 1 cut(s) 723
BfaI CTAG 1 cut(s) 332
BfmI CTRYAG 3 cut(s) 24, 45, 303
BisI GCNGC 3 cut(s) 303, 601, 604
BlsI GCNGC 3 cut(s) 304, 602, 605
Bme1390I CCNGG 1 cut(s) 504
BmiI GGNNCC 1 cut(s) 363
BmrFI CCNGG 1 cut(s) 504
BmrI ACTGGG 1 cut(s) 603
BmsI GCATC 4 cut(s) 18, 517, 655, 679
BmuI ACTGGG 1 cut(s) 603
BpiI GAAGAC 2 cut(s) 323, 417
Bpu10I CCTNAGC 1 cut(s) 205
BsaJI CCNNGG 1 cut(s) 151
Bsc4I CCNNNNNNNGG 4 cut(s) 280, 357, 358, 371
Bse1I ACTGG 3 cut(s) 58, 390, 598
BseBI CCWGG 1 cut(s) 504
BseDI CCNNGG 1 cut(s) 151
BseGI GGATG 2 cut(s) 694, 697
BseLI CCNNNNNNNGG 4 cut(s) 280, 357, 358, 371
BseMII CTCAG 1 cut(s) 27
BseNI ACTGG 3 cut(s) 58, 390, 598
BseXI GCAGC 2 cut(s) 289, 587
BshFI GGCC 1 cut(s) 562
BslFI GGGAC 2 cut(s) 64, 334
BslI CCNNNNNNNGG 4 cut(s) 280, 357, 358, 371
BsmFI GGGAC 2 cut(s) 64, 334
BsnI GGCC 1 cut(s) 562
Bsp143I GATC 3 cut(s) 637, 723, 730
BspACI CCGC 2 cut(s) 603, 641
BspANI GGCC 1 cut(s) 562
BspCNI CTCAG 1 cut(s) 26
BspLI GGNNCC 1 cut(s) 363
BspMAI CTGCAG 2 cut(s) 49, 307
BspPI GGATC 1 cut(s) 632
BsrI ACTGG 3 cut(s) 58, 390, 598
BssECI CCNNGG 1 cut(s) 151
BssMI GATC 3 cut(s) 637, 723, 730
BssT1I CCWWGG 1 cut(s) 151
Bst2UI CCWGG 1 cut(s) 504
Bst4CI ACNGT 1 cut(s) 268
BstC8I GCNNGC 2 cut(s) 421, 497
BstDEI CTNAG 3 cut(s) 13, 179, 205
BstF5I GGATG 2 cut(s) 694, 697
BstKTI GATC 3 cut(s) 640, 726, 733
BstMBI GATC 3 cut(s) 637, 723, 730
BstNI CCWGG 1 cut(s) 504
BstNSI RCATGY 1 cut(s) 660
BstSCI CCNGG 1 cut(s) 502
BstSFI CTRYAG 3 cut(s) 24, 45, 303
BstV1I GCAGC 2 cut(s) 289, 587
BstV2I GAAGAC 2 cut(s) 323, 417
BsuRI GGCC 1 cut(s) 562
BtsCI GGATG 2 cut(s) 694, 697
BtsIMutI CAGTG 1 cut(s) 383
Cac8I GCNNGC 2 cut(s) 421, 497
CaiI CAGNNNCTG 1 cut(s) 53
Csp6I GTAC 1 cut(s) 127
CviAII CATG 4 cut(s) 223, 416, 424, 657
CviJI RGCY 7 cut(s) 57, 146, 188, 204, 487, 499, 562
CviKI_1 RGCY 7 cut(s) 57, 146, 188, 204, 487, 499, 562
CviQI GTAC 1 cut(s) 127
DdeI CTNAG 3 cut(s) 13, 179, 205
DpnI GATC 3 cut(s) 639, 725, 732
DpnII GATC 3 cut(s) 637, 723, 730
Eco130I CCWWGG 1 cut(s) 151
Eco147I AGGCCT 1 cut(s) 562
Eco57I CTGAAG 1 cut(s) 96
EcoRII CCWGG 1 cut(s) 502
EcoT14I CCWWGG 1 cut(s) 151
EcoT22I ATGCAT 1 cut(s) 532
ErhI CCWWGG 1 cut(s) 151
FaeI CATG 4 cut(s) 226, 419, 427, 660
FalI AAGNNNNNCTT 4 cut(s) 135, 167, 172, 204
FaqI GGGAC 2 cut(s) 64, 334
FatI CATG 4 cut(s) 222, 415, 423, 656
FbaI TGATCA 1 cut(s) 723
Fnu4HI GCNGC 3 cut(s) 303, 601, 604
FokI GGATG 2 cut(s) 684, 701
Fsp4HI GCNGC 3 cut(s) 303, 601, 604
FspBI CTAG 1 cut(s) 332
GluI GCNGC 3 cut(s) 303, 601, 604
HaeIII GGCC 1 cut(s) 562
Hin1II CATG 4 cut(s) 226, 419, 427, 660
HinfI GANTC 2 cut(s) 91, 584
Hpy166II GTNNAC 2 cut(s) 264, 271
Hpy188I TCNGA 3 cut(s) 16, 76, 706
Hpy188III TCNNGA 3 cut(s) 281, 509, 588
Hpy8I GTNNAC 2 cut(s) 264, 271
Hpy99I CGWCG 1 cut(s) 255
HpyAV CCTTC 4 cut(s) 97, 157, 573, 711
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4IV ACGT 1 cut(s) 441
HpyCH4V TGCA 8 cut(s) 47, 259, 305, 423, 524, 530, 547, 668
HpyF3I CTNAG 3 cut(s) 13, 179, 205
HpySE526I ACGT 1 cut(s) 441
Hsp92II CATG 4 cut(s) 226, 419, 427, 660
Ksp22I TGATCA 1 cut(s) 723
Kzo9I GATC 3 cut(s) 637, 723, 730
Lsp1109I GCAGC 2 cut(s) 289, 587
LweI GCATC 4 cut(s) 18, 517, 655, 679
MaeI CTAG 1 cut(s) 332
MaeII ACGT 1 cut(s) 441
MalI GATC 3 cut(s) 639, 725, 732
MboI GATC 3 cut(s) 637, 723, 730
MboII GAAGA 3 cut(s) 328, 355, 422
MluCI AATT 2 cut(s) 137, 452
MnlI CCTC 6 cut(s) 178, 375, 421, 548, 574, 606
Mph1103I ATGCAT 1 cut(s) 532
MseI TTAA 3 cut(s) 140, 456, 467
MslI CAYNNNNRTG 1 cut(s) 655
MspR9I CCNGG 1 cut(s) 504
MvaI CCWGG 1 cut(s) 504
NdeII GATC 3 cut(s) 637, 723, 730
NlaIII CATG 4 cut(s) 226, 419, 427, 660
NlaIV GGNNCC 1 cut(s) 363
NsiI ATGCAT 1 cut(s) 532
NspI RCATGY 1 cut(s) 660
PceI AGGCCT 1 cut(s) 562
PfeI GAWTC 2 cut(s) 91, 584
PkrI GCNGC 3 cut(s) 304, 602, 605
Psp1406I AACGTT 1 cut(s) 441
Psp6I CCWGG 1 cut(s) 502
PspGI CCWGG 1 cut(s) 502
PspN4I GGNNCC 1 cut(s) 363
PstI CTGCAG 2 cut(s) 49, 307
PstNI CAGNNNCTG 1 cut(s) 53
RsaI GTAC 1 cut(s) 128
RsaNI GTAC 1 cut(s) 127
RseI CAYNNNNRTG 1 cut(s) 655
SaqAI TTAA 3 cut(s) 140, 456, 467
SatI GCNGC 3 cut(s) 303, 601, 604
Sau3AI GATC 3 cut(s) 637, 723, 730
ScrFI CCNGG 1 cut(s) 504
SetI ASST 7 cut(s) 153, 206, 444, 489, 501, 540, 617
SfaNI GCATC 4 cut(s) 18, 517, 655, 679
SfcI CTRYAG 3 cut(s) 24, 45, 303
SmiMI CAYNNNNRTG 1 cut(s) 655
Sse9I AATT 2 cut(s) 137, 452
SseBI AGGCCT 1 cut(s) 562
SsiI CCGC 2 cut(s) 603, 641
SspI AATATT 1 cut(s) 403
SspMI CTAG 1 cut(s) 332
StuI AGGCCT 1 cut(s) 562
StyD4I CCNGG 1 cut(s) 502
StyI CCWWGG 1 cut(s) 151
TaaI ACNGT 1 cut(s) 268
TaiI ACGT 1 cut(s) 444
TaqI TCGA 1 cut(s) 718
TasI AATT 2 cut(s) 137, 452
TauI GCSGC 1 cut(s) 606
TfiI GAWTC 2 cut(s) 91, 584
Tru1I TTAA 3 cut(s) 140, 456, 467
Tru9I TTAA 3 cut(s) 140, 456, 467
TscAI CASTG 1 cut(s) 390
TseI GCWGC 2 cut(s) 302, 600
TspDTI ATGAA 2 cut(s) 211, 686
TspRI CASTG 1 cut(s) 390
XapI RAATTY 1 cut(s) 452
XceI RCATGY 1 cut(s) 660
XcmI CCANNNNNNNNNTGG 1 cut(s) 403
XspI CTAG 1 cut(s) 332
Zsp2I ATGCAT 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.