RLG00000020610

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0013813
Physical Location & Seq
Forward (+)
66710916 .. 66712391
1476 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020610

Sequence Viewer

Length: 999 bp
ATGGCAAACTTGTTGATTCCTTGGTTATTTTTAGTTCAAACACTAACAATTGTTGCCAAATCCAGAGCTAAAGTTCCAGCAGTCATAGTGTTTGGAGATTCCTCCGTTGATTCAGGTAACAACAACTTCATTCCAACAATTGCCAGGAGCAACTTTCCACCTTATGGTCAAGATTTTCCGGGAGGCCAAGCCACCGGGCGGTTCTGCAACGGCCGAATTCCTCCTGACTTGCTCTCCGAAGCTCTAGGCCTCAAGCCAACCATACCTGCATACTTGGATCCAATGTACAGCATCTCAGATTTTGCTGTAGGAGTTTGCTTTGCTTCTGCAGGGACTGGCTATGATAATGCCACTTCTGAAGTTGCTGATGTGATTCCATTGTGGAAGGAAGTGGAGTATTACAAGGAGTACCAGCAGAAATTAAAAGCCTACCTTGGAGATAGGAAGGCAAAGAAAATACTAAGTGAGGCTTTGTATTTGATTAGCTTAGGAACAAATGACTTCATGGAAAACTATTTTACACTCCCAAACCGACGATTGCAGTTTACCACAATATATGCTTTAGGGGCAAGGAAGATATCCCTGACAGGGGTTCCTCCAATGGGGTGTTTGCCACTGGAAAGAACCACAAATATTATGGAAGACCATGCTTGTAGGGAGGAATACAACAACGTTGGTTTGGAATTTAATGGCAAGTTAAAGGGTTTGGTGGCAAAGCTGAACAGGGAGCTTCCTGGTCTTGATGTGGTATTTGCAGATGCATATAACCTCTTGTTGCAACTCATAAAAAGGCCTTCTGTTTATGGATTTGAGGAATCAAGAACTGGGTGCTGTGGCACAGGGAGGTTTGAGATGGGTTTCCTATGTGATCTGAATGGTGCATTTACATGCCAAGATGCAGATAAGTATGTGTTTTGGGATGCCTTTCATCCTTCGGAGAAAACCAATCGAATGATCACAGATCATCTACTCAAAACTTCTTTAGCGAAGTTTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

333

Amino Acids

36.93

Weight (kDa)

6.35

Isoelectric Point (pI)

27.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 28 - 322 3.3e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013813)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42990
fragaria_vesca FvH4_6g38660 FvH4_6g38660
malus_domestica MD09G1148500.v1.1
prunus_persica Prupe.3G177600_v2.0.a1
pyrus_communis pycom09g06820
rosa_chinensis RchiOBHm_Chr2g0152121
rosa_laevigata RLG00000020610
rosa_multiflora Rmu_sc0000760.1_g000001 Rmu_sc0003043.1_g000023
rosa_roxburghii Rroxscaffold_2G00095910
rosa_rugosa Rorug02G0431200
rosa_samantha Rh2AG492000 Rh2BG504500 Rh2CG478500 Rh2DG515800
rosa_wichuraiana Rw2G040440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 274
AccB7I CCANNNNNTGG 1 cut(s) 164
AciI CCGC 1 cut(s) 199
AclI AACGTT 1 cut(s) 672
AclWI GGATC 2 cut(s) 272, 285
AcoI YGGCCR 1 cut(s) 211
AcsI RAATTY 2 cut(s) 216, 683
AcuI CTGAAG 1 cut(s) 378
AfaI GTAC 2 cut(s) 287, 410
AfiI CCNNNNNNNGG 5 cut(s) 164, 198, 588, 589, 602
AgsI TTSAA 1 cut(s) 38
AjnI CCWGG 2 cut(s) 143, 733
AluBI AGCT 5 cut(s) 68, 242, 486, 718, 730
AluI AGCT 5 cut(s) 68, 242, 486, 718, 730
AlwI GGATC 2 cut(s) 272, 285
AlwNI CAGNNNCTG 1 cut(s) 335
AoxI GGCC 4 cut(s) 184, 211, 247, 791
ApoI RAATTY 2 cut(s) 216, 683
AsuC2I CCSGG 2 cut(s) 180, 196
BamHI GGATCC 1 cut(s) 277
BbsI GAAGAC 1 cut(s) 648
BccI CCATC 1 cut(s) 847
BceAI ACGGC 1 cut(s) 226
BciT130I CCWGG 2 cut(s) 145, 735
BclI TGATCA 1 cut(s) 954
BcnI CCSGG 2 cut(s) 180, 196
BfaI CTAG 1 cut(s) 245
BfmI CTRYAG 2 cut(s) 306, 327
BfuAI ACCTGC 1 cut(s) 274
Bme1390I CCNGG 4 cut(s) 145, 180, 196, 735
BmiI GGNNCC 2 cut(s) 279, 594
BmrFI CCNGG 4 cut(s) 145, 180, 196, 735
BmrI ACTGGG 1 cut(s) 834
BmsI GCATC 4 cut(s) 300, 748, 886, 910
BmuI ACTGGG 1 cut(s) 834
BpiI GAAGAC 1 cut(s) 648
Bpu10I CCTNAGC 1 cut(s) 487
BpuEI CTTGAG 1 cut(s) 236
BpuMI CCSGG 2 cut(s) 180, 196
BsaJI CCNNGG 2 cut(s) 20, 433
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 5 cut(s) 164, 198, 588, 589, 602
Bse1I ACTGG 3 cut(s) 340, 621, 829
BseBI CCWGG 2 cut(s) 145, 735
BseDI CCNNGG 2 cut(s) 20, 433
BseGI GGATG 2 cut(s) 925, 928
BseLI CCNNNNNNNGG 5 cut(s) 164, 198, 588, 589, 602
BseMII CTCAG 1 cut(s) 309
BseNI ACTGG 3 cut(s) 340, 621, 829
BseX3I CGGCCG 1 cut(s) 211
Bsh1285I CGRYCG 1 cut(s) 214
BshFI GGCC 4 cut(s) 186, 213, 249, 793
BsiEI CGRYCG 1 cut(s) 214
BsiSI CCGG 2 cut(s) 179, 195
BslFI GGGAC 1 cut(s) 346
BslI CCNNNNNNNGG 5 cut(s) 164, 198, 588, 589, 602
BsmFI GGGAC 1 cut(s) 346
BsnI GGCC 4 cut(s) 186, 213, 249, 793
Bsp1407I TGTACA 1 cut(s) 285
Bsp143I GATC 4 cut(s) 277, 868, 954, 961
BspACI CCGC 1 cut(s) 199
BspANI GGCC 4 cut(s) 186, 213, 249, 793
BspCNI CTCAG 1 cut(s) 308
BspLI GGNNCC 2 cut(s) 279, 594
BspMAI CTGCAG 1 cut(s) 331
BspMI ACCTGC 1 cut(s) 274
BspPI GGATC 2 cut(s) 272, 285
BsrGI TGTACA 1 cut(s) 285
BsrI ACTGG 3 cut(s) 340, 621, 829
BssECI CCNNGG 2 cut(s) 20, 433
BssMI GATC 4 cut(s) 277, 868, 954, 961
BssT1I CCWWGG 2 cut(s) 20, 433
Bst2UI CCWGG 2 cut(s) 145, 735
BstAUI TGTACA 1 cut(s) 285
BstDEI CTNAG 3 cut(s) 295, 461, 487
BstF5I GGATG 2 cut(s) 925, 928
BstKTI GATC 4 cut(s) 280, 871, 957, 964
BstMBI GATC 4 cut(s) 277, 868, 954, 961
BstMCI CGRYCG 1 cut(s) 214
BstMWI GCNNNNNNNGC 1 cut(s) 566
BstNI CCWGG 2 cut(s) 145, 735
BstNSI RCATGY 1 cut(s) 891
BstSCI CCNGG 4 cut(s) 143, 178, 194, 733
BstSFI CTRYAG 2 cut(s) 306, 327
BstV2I GAAGAC 1 cut(s) 648
BstX2I RGATCY 1 cut(s) 277
BstYI RGATCY 1 cut(s) 277
BstZI CGGCCG 1 cut(s) 211
BsuRI GGCC 4 cut(s) 186, 213, 249, 793
BtsCI GGATG 2 cut(s) 925, 928
BtsIMutI CAGTG 1 cut(s) 614
BveI ACCTGC 1 cut(s) 274
CaiI CAGNNNCTG 1 cut(s) 335
Csp6I GTAC 2 cut(s) 286, 409
CviAII CATG 3 cut(s) 505, 647, 888
CviQI GTAC 2 cut(s) 286, 409
DdeI CTNAG 3 cut(s) 295, 461, 487
DpnI GATC 4 cut(s) 279, 870, 956, 963
DpnII GATC 4 cut(s) 277, 868, 954, 961
EaeI YGGCCR 1 cut(s) 211
EagI CGGCCG 1 cut(s) 211
EclXI CGGCCG 1 cut(s) 211
Eco130I CCWWGG 2 cut(s) 20, 433
Eco147I AGGCCT 2 cut(s) 249, 793
Eco32I GATATC 1 cut(s) 579
Eco52I CGGCCG 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 378
EcoRI GAATTC 1 cut(s) 216
EcoRII CCWGG 2 cut(s) 143, 733
EcoRV GATATC 1 cut(s) 579
EcoT14I CCWWGG 2 cut(s) 20, 433
EcoT22I ATGCAT 1 cut(s) 763
ErhI CCWWGG 2 cut(s) 20, 433
FaeI CATG 3 cut(s) 508, 650, 891
FalI AAGNNNNNCTT 4 cut(s) 417, 449, 454, 486
FaqI GGGAC 1 cut(s) 346
FatI CATG 3 cut(s) 504, 646, 887
FbaI TGATCA 1 cut(s) 954
FokI GGATG 2 cut(s) 915, 932
FspBI CTAG 1 cut(s) 245
HaeIII GGCC 4 cut(s) 186, 213, 249, 793
HapII CCGG 2 cut(s) 179, 195
Hin1II CATG 3 cut(s) 508, 650, 891
HinfI GANTC 5 cut(s) 16, 98, 110, 373, 815
HpaII CCGG 2 cut(s) 179, 195
Hpy166II GTNNAC 1 cut(s) 546
Hpy188I TCNGA 5 cut(s) 238, 298, 358, 873, 937
Hpy188III TCNNGA 5 cut(s) 63, 170, 224, 740, 819
Hpy8I GTNNAC 1 cut(s) 546
Hpy99I CGWCG 1 cut(s) 537
HpyAV CCTTC 4 cut(s) 379, 439, 804, 942
HpyCH4IV ACGT 1 cut(s) 672
HpyCH4V TGCA 9 cut(s) 207, 269, 329, 541, 755, 761, 778, 881, 899
HpyF10VI GCNNNNNNNGC 1 cut(s) 566
HpyF3I CTNAG 3 cut(s) 295, 461, 487
HpySE526I ACGT 1 cut(s) 672
Hsp92II CATG 3 cut(s) 508, 650, 891
Ksp22I TGATCA 1 cut(s) 954
Kzo9I GATC 4 cut(s) 277, 868, 954, 961
LmnI GCTCC 2 cut(s) 147, 727
LweI GCATC 4 cut(s) 300, 748, 886, 910
MaeI CTAG 1 cut(s) 245
MaeII ACGT 1 cut(s) 672
MaeIII GTNAC 1 cut(s) 116
MalI GATC 4 cut(s) 279, 870, 956, 963
MboI GATC 4 cut(s) 277, 868, 954, 961
MboII GAAGA 2 cut(s) 586, 653
MfeI CAATTG 2 cut(s) 48, 138
MflI RGATCY 1 cut(s) 277
MluCI AATT 5 cut(s) 48, 138, 216, 419, 683
MmeI TCCRAC 1 cut(s) 158
Mph1103I ATGCAT 1 cut(s) 763
MseI TTAA 3 cut(s) 422, 687, 698
MslI CAYNNNNRTG 1 cut(s) 886
MspI CCGG 2 cut(s) 179, 195
MspR9I CCNGG 4 cut(s) 145, 180, 196, 735
MunI CAATTG 2 cut(s) 48, 138
MvaI CCWGG 2 cut(s) 145, 735
MwoI GCNNNNNNNGC 1 cut(s) 566
NciI CCSGG 2 cut(s) 180, 196
NdeII GATC 4 cut(s) 277, 868, 954, 961
NlaIII CATG 3 cut(s) 508, 650, 891
NlaIV GGNNCC 2 cut(s) 279, 594
NsiI ATGCAT 1 cut(s) 763
NspI RCATGY 1 cut(s) 891
PceI AGGCCT 2 cut(s) 249, 793
PfeI GAWTC 5 cut(s) 16, 98, 110, 373, 815
PflMI CCANNNNNTGG 1 cut(s) 164
PfoI TCCNGGA 1 cut(s) 178
Psp1406I AACGTT 1 cut(s) 672
Psp6I CCWGG 2 cut(s) 143, 733
PspGI CCWGG 2 cut(s) 143, 733
PspN4I GGNNCC 2 cut(s) 279, 594
PstI CTGCAG 1 cut(s) 331
PstNI CAGNNNCTG 1 cut(s) 335
PsuI RGATCY 1 cut(s) 277
RsaI GTAC 2 cut(s) 287, 410
RsaNI GTAC 2 cut(s) 286, 409
RseI CAYNNNNRTG 1 cut(s) 886
SaqAI TTAA 3 cut(s) 422, 687, 698
Sau3AI GATC 4 cut(s) 277, 868, 954, 961
ScrFI CCNGG 4 cut(s) 145, 180, 196, 735
SfaNI GCATC 4 cut(s) 300, 748, 886, 910
SfcI CTRYAG 2 cut(s) 306, 327
SmiMI CAYNNNNRTG 1 cut(s) 886
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
Sse9I AATT 5 cut(s) 48, 138, 216, 419, 683
SseBI AGGCCT 2 cut(s) 249, 793
SsiI CCGC 1 cut(s) 199
SspI AATATT 1 cut(s) 634
SspMI CTAG 1 cut(s) 245
StuI AGGCCT 2 cut(s) 249, 793
StyD4I CCNGG 4 cut(s) 143, 178, 194, 733
StyI CCWWGG 2 cut(s) 20, 433
TaiI ACGT 1 cut(s) 675
TaqI TCGA 1 cut(s) 949
TasI AATT 5 cut(s) 48, 138, 216, 419, 683
TatI WGTACW 1 cut(s) 285
TfiI GAWTC 5 cut(s) 16, 98, 110, 373, 815
Tru1I TTAA 3 cut(s) 422, 687, 698
Tru9I TTAA 3 cut(s) 422, 687, 698
TscAI CASTG 1 cut(s) 621
TspDTI ATGAA 3 cut(s) 118, 493, 917
TspGWI ACGGA 1 cut(s) 94
TspRI CASTG 1 cut(s) 621
Van91I CCANNNNNTGG 1 cut(s) 164
XapI RAATTY 2 cut(s) 216, 683
XceI RCATGY 1 cut(s) 891
XcmI CCANNNNNNNNNTGG 1 cut(s) 634
XspI CTAG 1 cut(s) 245
Zsp2I ATGCAT 1 cut(s) 763
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.