Rw2G040440

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0013813
Physical Location & Seq
Forward (+)
65939323 .. 65940775
1453 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G040440.1

Sequence Viewer

Length: 1050 bp
ATGGCAAACTTGTTGATTCCTTGGCTATTTTTAGTTCAAACACTAACAATTGTTGCCAAATCCAGAGCTAAAGTTCCAGCAGTCATAGTCTTTGGAGATTCCTCCGTTGATTCAGGTAACAACAACTTCATTCCAACAATTGCCAGGAGCAACTTTCCACCTTATGGTCAAGATTTTCCGGGAGGCCAAGCCACCGGGCGGTTCTGCAACGGCCGAATTCCTCCTGACTTGATCTCCGAAGCTCTAGGCCTCAAGCCAACCATACCTGCATACTTGGATCCAATGTATAGCATCTCAGATTTTGCTGTAGGAGTTTGCTTTGCTTCTGCAGGGACTGGCTATGATAATGCCACTTCTGAAGTTGCTGATGTGATTCCATTGTGGAAGGAAGTGGAGTATTACAAGGAGTACCAGCAGAAATTAAAAGCCTACCTTGGAGATAGGAAGGCAAAGAAAATACTAAGTGAGGCTTTGTATTTGATTAGCTTAGGAACAAATGACTTCATGGAAAACTATTATACACTCCCAAACCGACGATTGCAGTTTACCGTGAACCAATATCAGGATTTTATTATAGGACTTGCTGCAGATTTTGTGAAGACAATATATTCTTTAGGGGCAAGGAAGATGTCCCTGACAGGGGTTCCTCCAATGGGGTGTTTGCCACTGGAAAGAACCACAAATATTATGGAAGACCATGCTTGCATGGAGGAATACAACAACGTTGGTTTGGAATTTAATGGCAAGTTAAAGGGTTTGGTGGCAAAGCTGAACAGCGAGCTTCCTGGTCTTGATGTGGTATTTGCAGATGCATATAACCTCTTGTTGCAAGTCATAAAAAGGCCTTCTGTTTATGGATTTGAGGAATCAAGAACTGGGTGCTGCGGCACAGGGAGGTTTGAGATGAGTTTCCTATGTGATCCGCATAGTCCATTTACATGCCAAGATGCAGATAAGTATGTGTTTTGGGATGCCTTTCATCCTTCGGAGAAAACCAATCGAATGATCACAGATCATCTACTCAAAACTTCTTTAGCAAAGTTTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

349

Amino Acids

38.89

Weight (kDa)

5.68

Isoelectric Point (pI)

32.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 28 - 339 1.4e-34 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013813)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42990
fragaria_vesca FvH4_6g38660 FvH4_6g38660
malus_domestica MD09G1148500.v1.1
prunus_persica Prupe.3G177600_v2.0.a1
pyrus_communis pycom09g06820
rosa_chinensis RchiOBHm_Chr2g0152121
rosa_laevigata RLG00000020610
rosa_multiflora Rmu_sc0000760.1_g000001 Rmu_sc0003043.1_g000023
rosa_roxburghii Rroxscaffold_2G00095910
rosa_rugosa Rorug02G0431200
rosa_samantha Rh2AG492000 Rh2BG504500 Rh2CG478500 Rh2DG515800
rosa_wichuraiana Rw2G040440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 274
AccB7I CCANNNNNTGG 1 cut(s) 164
AciI CCGC 3 cut(s) 199, 885, 923
AclI AACGTT 1 cut(s) 723
AclWI GGATC 3 cut(s) 272, 285, 914
AcoI YGGCCR 1 cut(s) 211
AcsI RAATTY 2 cut(s) 216, 734
AcuI CTGAAG 1 cut(s) 378
AfaI GTAC 1 cut(s) 410
AfiI CCNNNNNNNGG 6 cut(s) 164, 198, 562, 639, 640, 653
AgsI TTSAA 1 cut(s) 38
AjnI CCWGG 2 cut(s) 143, 784
AluBI AGCT 5 cut(s) 68, 242, 486, 769, 781
AluI AGCT 5 cut(s) 68, 242, 486, 769, 781
AlwI GGATC 3 cut(s) 272, 285, 914
AlwNI CAGNNNCTG 1 cut(s) 335
AoxI GGCC 4 cut(s) 184, 211, 247, 842
ApeKI GCWGC 2 cut(s) 584, 882
ApoI RAATTY 2 cut(s) 216, 734
AsuC2I CCSGG 2 cut(s) 180, 196
BamHI GGATCC 1 cut(s) 277
BbsI GAAGAC 2 cut(s) 605, 699
BbvI GCAGC 2 cut(s) 571, 869
BceAI ACGGC 1 cut(s) 226
BciT130I CCWGG 2 cut(s) 145, 786
BclI TGATCA 1 cut(s) 1005
BcnI CCSGG 2 cut(s) 180, 196
BfaI CTAG 1 cut(s) 245
BfmI CTRYAG 3 cut(s) 306, 327, 585
BfuAI ACCTGC 1 cut(s) 274
BisI GCNGC 3 cut(s) 585, 883, 886
BlsI GCNGC 3 cut(s) 586, 884, 887
Bme1390I CCNGG 4 cut(s) 145, 180, 196, 786
BmiI GGNNCC 2 cut(s) 279, 645
BmrFI CCNGG 4 cut(s) 145, 180, 196, 786
BmrI ACTGGG 1 cut(s) 885
BmsI GCATC 4 cut(s) 300, 799, 937, 961
BmuI ACTGGG 1 cut(s) 885
BpiI GAAGAC 2 cut(s) 605, 699
Bpu10I CCTNAGC 1 cut(s) 487
BpuEI CTTGAG 1 cut(s) 236
BpuMI CCSGG 2 cut(s) 180, 196
BsaJI CCNNGG 2 cut(s) 20, 433
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 6 cut(s) 164, 198, 562, 639, 640, 653
Bse1I ACTGG 3 cut(s) 340, 672, 880
BseBI CCWGG 2 cut(s) 145, 786
BseDI CCNNGG 2 cut(s) 20, 433
BseGI GGATG 2 cut(s) 976, 979
BseLI CCNNNNNNNGG 6 cut(s) 164, 198, 562, 639, 640, 653
BseMII CTCAG 1 cut(s) 309
BseNI ACTGG 3 cut(s) 340, 672, 880
BseX3I CGGCCG 1 cut(s) 211
BseXI GCAGC 2 cut(s) 571, 869
Bsh1285I CGRYCG 1 cut(s) 214
BshFI GGCC 4 cut(s) 186, 213, 249, 844
BsiEI CGRYCG 1 cut(s) 214
BsiSI CCGG 2 cut(s) 179, 195
BslFI GGGAC 2 cut(s) 346, 616
BslI CCNNNNNNNGG 6 cut(s) 164, 198, 562, 639, 640, 653
BsmFI GGGAC 2 cut(s) 346, 616
BsnI GGCC 4 cut(s) 186, 213, 249, 844
Bsp143I GATC 5 cut(s) 231, 277, 919, 1005, 1012
BspACI CCGC 3 cut(s) 199, 885, 923
BspANI GGCC 4 cut(s) 186, 213, 249, 844
BspCNI CTCAG 1 cut(s) 308
BspLI GGNNCC 2 cut(s) 279, 645
BspMAI CTGCAG 2 cut(s) 331, 589
BspMI ACCTGC 1 cut(s) 274
BspPI GGATC 3 cut(s) 272, 285, 914
BsrI ACTGG 3 cut(s) 340, 672, 880
BssECI CCNNGG 2 cut(s) 20, 433
BssMI GATC 5 cut(s) 231, 277, 919, 1005, 1012
BssT1I CCWWGG 2 cut(s) 20, 433
Bst2UI CCWGG 2 cut(s) 145, 786
Bst4CI ACNGT 1 cut(s) 550
BstC8I GCNNGC 2 cut(s) 703, 779
BstDEI CTNAG 3 cut(s) 295, 461, 487
BstF5I GGATG 2 cut(s) 976, 979
BstKTI GATC 5 cut(s) 234, 280, 922, 1008, 1015
BstMBI GATC 5 cut(s) 231, 277, 919, 1005, 1012
BstMCI CGRYCG 1 cut(s) 214
BstNI CCWGG 2 cut(s) 145, 786
BstNSI RCATGY 1 cut(s) 942
BstSCI CCNGG 4 cut(s) 143, 178, 194, 784
BstSFI CTRYAG 3 cut(s) 306, 327, 585
BstV1I GCAGC 2 cut(s) 571, 869
BstV2I GAAGAC 2 cut(s) 605, 699
BstX2I RGATCY 1 cut(s) 277
BstYI RGATCY 1 cut(s) 277
BstZI CGGCCG 1 cut(s) 211
BsuRI GGCC 4 cut(s) 186, 213, 249, 844
BtsCI GGATG 2 cut(s) 976, 979
BtsIMutI CAGTG 1 cut(s) 665
BveI ACCTGC 1 cut(s) 274
Cac8I GCNNGC 2 cut(s) 703, 779
CaiI CAGNNNCTG 1 cut(s) 335
Csp6I GTAC 1 cut(s) 409
CviAII CATG 4 cut(s) 505, 698, 706, 939
CviQI GTAC 1 cut(s) 409
DdeI CTNAG 3 cut(s) 295, 461, 487
DpnI GATC 5 cut(s) 233, 279, 921, 1007, 1014
DpnII GATC 5 cut(s) 231, 277, 919, 1005, 1012
EaeI YGGCCR 1 cut(s) 211
EagI CGGCCG 1 cut(s) 211
EclXI CGGCCG 1 cut(s) 211
Eco130I CCWWGG 2 cut(s) 20, 433
Eco147I AGGCCT 2 cut(s) 249, 844
Eco52I CGGCCG 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 378
EcoRI GAATTC 1 cut(s) 216
EcoRII CCWGG 2 cut(s) 143, 784
EcoT14I CCWWGG 2 cut(s) 20, 433
EcoT22I ATGCAT 1 cut(s) 814
ErhI CCWWGG 2 cut(s) 20, 433
FaeI CATG 4 cut(s) 508, 701, 709, 942
FalI AAGNNNNNCTT 4 cut(s) 417, 449, 454, 486
FaqI GGGAC 2 cut(s) 346, 616
FatI CATG 4 cut(s) 504, 697, 705, 938
FbaI TGATCA 1 cut(s) 1005
Fnu4HI GCNGC 3 cut(s) 585, 883, 886
FokI GGATG 2 cut(s) 966, 983
Fsp4HI GCNGC 3 cut(s) 585, 883, 886
FspBI CTAG 1 cut(s) 245
GluI GCNGC 3 cut(s) 585, 883, 886
HaeIII GGCC 4 cut(s) 186, 213, 249, 844
HapII CCGG 2 cut(s) 179, 195
Hin1II CATG 4 cut(s) 508, 701, 709, 942
HinfI GANTC 5 cut(s) 16, 98, 110, 373, 866
HpaII CCGG 2 cut(s) 179, 195
Hpy166II GTNNAC 2 cut(s) 546, 553
Hpy188I TCNGA 4 cut(s) 238, 298, 358, 988
Hpy188III TCNNGA 6 cut(s) 63, 170, 224, 563, 791, 870
Hpy8I GTNNAC 2 cut(s) 546, 553
Hpy99I CGWCG 1 cut(s) 537
HpyAV CCTTC 4 cut(s) 379, 439, 855, 993
HpyCH4III ACNGT 1 cut(s) 550
HpyCH4IV ACGT 1 cut(s) 723
HpyF3I CTNAG 3 cut(s) 295, 461, 487
HpySE526I ACGT 1 cut(s) 723
Hsp92II CATG 4 cut(s) 508, 701, 709, 942
Ksp22I TGATCA 1 cut(s) 1005
Kzo9I GATC 5 cut(s) 231, 277, 919, 1005, 1012
LmnI GCTCC 1 cut(s) 147
Lsp1109I GCAGC 2 cut(s) 571, 869
LweI GCATC 4 cut(s) 300, 799, 937, 961
MaeI CTAG 1 cut(s) 245
MaeII ACGT 1 cut(s) 723
MaeIII GTNAC 1 cut(s) 116
MalI GATC 5 cut(s) 233, 279, 921, 1007, 1014
MboI GATC 5 cut(s) 231, 277, 919, 1005, 1012
MboII GAAGA 3 cut(s) 610, 637, 704
MfeI CAATTG 2 cut(s) 48, 138
MflI RGATCY 1 cut(s) 277
MluCI AATT 5 cut(s) 48, 138, 216, 419, 734
MmeI TCCRAC 1 cut(s) 158
Mph1103I ATGCAT 1 cut(s) 814
MseI TTAA 3 cut(s) 422, 738, 749
MslI CAYNNNNRTG 1 cut(s) 937
MspI CCGG 2 cut(s) 179, 195
MspR9I CCNGG 4 cut(s) 145, 180, 196, 786
MunI CAATTG 2 cut(s) 48, 138
MvaI CCWGG 2 cut(s) 145, 786
NciI CCSGG 2 cut(s) 180, 196
NdeII GATC 5 cut(s) 231, 277, 919, 1005, 1012
NlaIII CATG 4 cut(s) 508, 701, 709, 942
NlaIV GGNNCC 2 cut(s) 279, 645
NsiI ATGCAT 1 cut(s) 814
NspI RCATGY 1 cut(s) 942
PceI AGGCCT 2 cut(s) 249, 844
PfeI GAWTC 5 cut(s) 16, 98, 110, 373, 866
PflMI CCANNNNNTGG 1 cut(s) 164
PfoI TCCNGGA 1 cut(s) 178
PkrI GCNGC 3 cut(s) 586, 884, 887
Psp1406I AACGTT 1 cut(s) 723
Psp6I CCWGG 2 cut(s) 143, 784
PspGI CCWGG 2 cut(s) 143, 784
PspN4I GGNNCC 2 cut(s) 279, 645
PstI CTGCAG 2 cut(s) 331, 589
PstNI CAGNNNCTG 1 cut(s) 335
PsuI RGATCY 1 cut(s) 277
RsaI GTAC 1 cut(s) 410
RsaNI GTAC 1 cut(s) 409
RseI CAYNNNNRTG 1 cut(s) 937
SaqAI TTAA 3 cut(s) 422, 738, 749
SatI GCNGC 3 cut(s) 585, 883, 886
Sau3AI GATC 5 cut(s) 231, 277, 919, 1005, 1012
ScrFI CCNGG 4 cut(s) 145, 180, 196, 786
SfaNI GCATC 4 cut(s) 300, 799, 937, 961
SfcI CTRYAG 3 cut(s) 306, 327, 585
SmiMI CAYNNNNRTG 1 cut(s) 937
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
Sse9I AATT 5 cut(s) 48, 138, 216, 419, 734
SseBI AGGCCT 2 cut(s) 249, 844
SsiI CCGC 3 cut(s) 199, 885, 923
SspI AATATT 1 cut(s) 685
SspMI CTAG 1 cut(s) 245
StuI AGGCCT 2 cut(s) 249, 844
StyD4I CCNGG 4 cut(s) 143, 178, 194, 784
StyI CCWWGG 2 cut(s) 20, 433
TaaI ACNGT 1 cut(s) 550
TaiI ACGT 1 cut(s) 726
TaqI TCGA 1 cut(s) 1000
TasI AATT 5 cut(s) 48, 138, 216, 419, 734
TauI GCSGC 1 cut(s) 888
TfiI GAWTC 5 cut(s) 16, 98, 110, 373, 866
Tru1I TTAA 3 cut(s) 422, 738, 749
Tru9I TTAA 3 cut(s) 422, 738, 749
TscAI CASTG 1 cut(s) 672
TseI GCWGC 2 cut(s) 584, 882
TspDTI ATGAA 3 cut(s) 118, 493, 968
TspGWI ACGGA 1 cut(s) 94
TspRI CASTG 1 cut(s) 672
Van91I CCANNNNNTGG 1 cut(s) 164
XapI RAATTY 2 cut(s) 216, 734
XceI RCATGY 1 cut(s) 942
XcmI CCANNNNNNNNNTGG 1 cut(s) 685
XspI CTAG 1 cut(s) 245
Zsp2I ATGCAT 1 cut(s) 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.