FvH4_6g38660

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013813
Physical Location & Seq
Forward (+)
30599740 .. 30601419
1680 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g38660.t1

Sequence Viewer

Length: 1050 bp
ATGAGAAACTTGTTAGTTCCTTGGCTATTTCTAGTTCAAACACTAGTAGTTGTTGCCATATCAGGAGCTAAAGTTCCAGCAGTCATAGTGTTTGGAGATTCCTCTCTTGACTCAGGCAACAACAACTTCATTCCAACAATTGCCAGGAGCAACTTTGCACCTTATGGTAAAGATTTTCCGGGAGGCCAAGCGACTGGGCGATTCTGCAATGGCAGAATTCCTTCTGACATGATCTCTGAAGCTCTAGGCCTCAAGCCAACCGTACCTGCATACTTGGATCCAATGTATAACATTTCGGATTTTGCTGTCGGAGTTTGCTTTGCTTCTGCAGGGACTGGCTATGATAATACCACTTCTGATGTTGTAGATGTGATTCCATTGTGGAAGGAAGTGGAGTACTATAAGGAGTACCAGAAGAAATTGAAAGCTTATCTTGGAGATAGGAAGGCAAAGAAAATACTGAATGAGGCTTTGTATTTGATTAGCATAGGAACAAATGACTTCCTAGAAAACTATTATACACTCCCGAACCGGAGATCGCAGTTCACGGTGAACCAATATCAGGATTTCATCATAGGGCTTGCTGAAGATTTTGTGAAGACAATATATGCTTTAGGAGCAAGGAGGATGTCCCTAACAGGAGTTCCTCCAATGGGGTGTTTGCCGCTGGAAAGAACCACAAATATAATGGAGGACCATGCTTGTATGGAGGAATACAACATTGTTGCTTTGGAATACAATGGCAAGTTAAAGGGTTTGGTGGCAAAACTGAACAGGGAGCTTCCTGGTCTTGATGTGGTATTTGCAGATGCATATCACGTTTTGTTGCGACTCATAAAAAAGCCTTCTCTTTATGGATTTGAGGAATCCAAAACTGGGTGCTGTGGCACAGGGAGGTTTGAGATGAGTTTCCTATGTGATCCATATAGTCCATTTACATGCCAAGATGCAGACAAGTATGTGTTTTGGGATGCCTTTCATCCTTCAGAGAAAACCAATCGAATGATTACAGATCATCTACTCAAAACTTCTTTCGCAAAGTTTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

350

Amino Acids

39.11

Weight (kDa)

6.14

Isoelectric Point (pI)

33.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 28 - 339 2.4e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013813)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42990
fragaria_vesca FvH4_6g38660 FvH4_6g38660
malus_domestica MD09G1148500.v1.1
prunus_persica Prupe.3G177600_v2.0.a1
pyrus_communis pycom09g06820
rosa_chinensis RchiOBHm_Chr2g0152121
rosa_laevigata RLG00000020610
rosa_multiflora Rmu_sc0000760.1_g000001 Rmu_sc0003043.1_g000023
rosa_roxburghii Rroxscaffold_2G00095910
rosa_rugosa Rorug02G0431200
rosa_samantha Rh2AG492000 Rh2BG504500 Rh2CG478500 Rh2DG515800
rosa_wichuraiana Rw2G040440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 274
AciI CCGC 1 cut(s) 665
AclWI GGATC 3 cut(s) 272, 285, 914
AcsI RAATTY 1 cut(s) 216
AcuI CTGAAG 3 cut(s) 258, 606, 969
AfaI GTAC 3 cut(s) 264, 398, 410
AfiI CCNNNNNNNGG 3 cut(s) 562, 653, 876
AgsI TTSAA 2 cut(s) 38, 424
AhlI ACTAGT 1 cut(s) 43
AjnI CCWGG 2 cut(s) 143, 784
AloI GAACNNNNNNTCC 2 cut(s) 57, 89
AluBI AGCT 4 cut(s) 68, 242, 428, 781
AluI AGCT 4 cut(s) 68, 242, 428, 781
AlwI GGATC 3 cut(s) 272, 285, 914
AlwNI CAGNNNCTG 1 cut(s) 335
AoxI GGCC 2 cut(s) 184, 247
ApoI RAATTY 1 cut(s) 216
Asp700I GAANNNNTTC 1 cut(s) 220
AspS9I GGNCC 1 cut(s) 694
AsuC2I CCSGG 1 cut(s) 180
AsuHPI GGTGA 1 cut(s) 562
AvaII GGWCC 1 cut(s) 694
BamHI GGATCC 1 cut(s) 277
BbsI GAAGAC 1 cut(s) 605
BciT130I CCWGG 2 cut(s) 145, 786
BcnI CCSGG 1 cut(s) 180
BcuI ACTAGT 1 cut(s) 43
BfaI CTAG 4 cut(s) 32, 44, 245, 506
BfmI CTRYAG 1 cut(s) 327
BfuAI ACCTGC 1 cut(s) 274
BisI GCNGC 1 cut(s) 665
BlsI GCNGC 1 cut(s) 666
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 3 cut(s) 145, 180, 786
Bme18I GGWCC 1 cut(s) 694
BmgT120I GGNCC 1 cut(s) 694
BmiI GGNNCC 1 cut(s) 279
BmrFI CCNGG 3 cut(s) 145, 180, 786
BmrI ACTGGG 2 cut(s) 204, 885
BmsI GCATC 3 cut(s) 799, 937, 961
BmuI ACTGGG 2 cut(s) 204, 885
BpiI GAAGAC 1 cut(s) 605
BpuEI CTTGAG 1 cut(s) 236
BpuMI CCSGG 1 cut(s) 180
BsaBI GATNNNNATC 1 cut(s) 813
BsaJI CCNNGG 1 cut(s) 20
BsaWI WCCGGW 1 cut(s) 531
BsaXI ACNNNNNCTCC 2 cut(s) 57, 87
Bsc4I CCNNNNNNNGG 3 cut(s) 562, 653, 876
Bse1I ACTGG 3 cut(s) 199, 340, 880
Bse3DI GCAATG 1 cut(s) 214
Bse8I GATNNNNATC 1 cut(s) 813
BseBI CCWGG 2 cut(s) 145, 786
BseDI CCNNGG 1 cut(s) 20
BseGI GGATG 3 cut(s) 633, 976, 979
BseJI GATNNNNATC 1 cut(s) 813
BseLI CCNNNNNNNGG 3 cut(s) 562, 653, 876
BseMI GCAATG 1 cut(s) 214
BseMII CTCAG 1 cut(s) 126
BseNI ACTGG 3 cut(s) 199, 340, 880
BshFI GGCC 2 cut(s) 186, 249
BsiSI CCGG 2 cut(s) 179, 532
BslFI GGGAC 2 cut(s) 346, 616
BslI CCNNNNNNNGG 3 cut(s) 562, 653, 876
BsmFI GGGAC 2 cut(s) 346, 616
BsnI GGCC 2 cut(s) 186, 249
Bsp143I GATC 5 cut(s) 231, 277, 536, 919, 1012
BspACI CCGC 1 cut(s) 665
BspANI GGCC 2 cut(s) 186, 249
BspCNI CTCAG 1 cut(s) 125
BspLI GGNNCC 1 cut(s) 279
BspMAI CTGCAG 1 cut(s) 331
BspMI ACCTGC 1 cut(s) 274
BspPI GGATC 3 cut(s) 272, 285, 914
BsrDI GCAATG 1 cut(s) 214
BsrI ACTGG 3 cut(s) 199, 340, 880
BssECI CCNNGG 1 cut(s) 20
BssMI GATC 5 cut(s) 231, 277, 536, 919, 1012
BssT1I CCWWGG 1 cut(s) 20
Bst2UI CCWGG 2 cut(s) 145, 786
Bst4CI ACNGT 2 cut(s) 262, 550
BstC8I GCNNGC 1 cut(s) 582
BstDEI CTNAG 1 cut(s) 112
BstF5I GGATG 3 cut(s) 633, 976, 979
BstKTI GATC 5 cut(s) 234, 280, 539, 922, 1015
BstMBI GATC 5 cut(s) 231, 277, 536, 919, 1012
BstMWI GCNNNNNNNGC 1 cut(s) 617
BstNI CCWGG 2 cut(s) 145, 786
BstNSI RCATGY 1 cut(s) 942
BstSCI CCNGG 3 cut(s) 143, 178, 784
BstSFI CTRYAG 1 cut(s) 327
BstV2I GAAGAC 1 cut(s) 605
BstX2I RGATCY 1 cut(s) 277
BstXI CCANNNNNNTGG 1 cut(s) 194
BstYI RGATCY 1 cut(s) 277
BsuRI GGCC 2 cut(s) 186, 249
BtsCI GGATG 3 cut(s) 633, 976, 979
BveI ACCTGC 1 cut(s) 274
Cac8I GCNNGC 1 cut(s) 582
CaiI CAGNNNCTG 1 cut(s) 335
Cfr13I GGNCC 1 cut(s) 694
Csp6I GTAC 3 cut(s) 263, 397, 409
CviAII CATG 3 cut(s) 229, 698, 939
CviQI GTAC 3 cut(s) 263, 397, 409
DdeI CTNAG 1 cut(s) 112
DpnI GATC 5 cut(s) 233, 279, 538, 921, 1014
DpnII GATC 5 cut(s) 231, 277, 536, 919, 1012
Eco130I CCWWGG 1 cut(s) 20
Eco147I AGGCCT 1 cut(s) 249
Eco47I GGWCC 1 cut(s) 694
Eco57I CTGAAG 3 cut(s) 258, 606, 969
EcoRI GAATTC 1 cut(s) 216
EcoRII CCWGG 2 cut(s) 143, 784
EcoT14I CCWWGG 1 cut(s) 20
EcoT22I ATGCAT 1 cut(s) 814
ErhI CCWWGG 1 cut(s) 20
FaeI CATG 3 cut(s) 232, 701, 942
FalI AAGNNNNNCTT 2 cut(s) 417, 449
FaqI GGGAC 2 cut(s) 346, 616
FatI CATG 3 cut(s) 228, 697, 938
Fnu4HI GCNGC 1 cut(s) 665
FokI GGATG 3 cut(s) 640, 966, 983
Fsp4HI GCNGC 1 cut(s) 665
FspBI CTAG 4 cut(s) 32, 44, 245, 506
GluI GCNGC 1 cut(s) 665
HaeIII GGCC 2 cut(s) 186, 249
HapII CCGG 2 cut(s) 179, 532
Hin1II CATG 3 cut(s) 232, 701, 942
HindIII AAGCTT 1 cut(s) 426
HinfI GANTC 6 cut(s) 98, 110, 201, 373, 831, 866
HpaII CCGG 2 cut(s) 179, 532
HphI GGTGA 1 cut(s) 562
Hpy166II GTNNAC 2 cut(s) 546, 553
Hpy188I TCNGA 6 cut(s) 226, 238, 298, 311, 358, 988
Hpy188III TCNNGA 5 cut(s) 63, 107, 526, 563, 791
Hpy8I GTNNAC 2 cut(s) 546, 553
HpyAV CCTTC 5 cut(s) 231, 379, 439, 855, 993
HpyCH4III ACNGT 2 cut(s) 262, 550
HpyCH4IV ACGT 1 cut(s) 819
HpyCH4V TGCA 7 cut(s) 158, 207, 269, 329, 806, 812, 950
HpyF10VI GCNNNNNNNGC 1 cut(s) 617
HpyF3I CTNAG 1 cut(s) 112
HpySE526I ACGT 1 cut(s) 819
Hsp92II CATG 3 cut(s) 232, 701, 942
Kzo9I GATC 5 cut(s) 231, 277, 536, 919, 1012
LmnI GCTCC 4 cut(s) 65, 147, 617, 778
LweI GCATC 3 cut(s) 799, 937, 961
MaeI CTAG 4 cut(s) 32, 44, 245, 506
MaeII ACGT 1 cut(s) 819
MalI GATC 5 cut(s) 233, 279, 538, 921, 1014
MboI GATC 5 cut(s) 231, 277, 536, 919, 1012
MboII GAAGA 3 cut(s) 427, 599, 610
MfeI CAATTG 1 cut(s) 138
MflI RGATCY 1 cut(s) 277
MluCI AATT 3 cut(s) 138, 216, 419
MlyI GAGTC 2 cut(s) 104, 825
MmeI TCCRAC 2 cut(s) 158, 289
Mph1103I ATGCAT 1 cut(s) 814
MroXI GAANNNNTTC 1 cut(s) 220
MseI TTAA 1 cut(s) 749
MslI CAYNNNNRTG 1 cut(s) 937
MspA1I CMGCKG 1 cut(s) 667
MspI CCGG 2 cut(s) 179, 532
MspR9I CCNGG 3 cut(s) 145, 180, 786
MunI CAATTG 1 cut(s) 138
MvaI CCWGG 2 cut(s) 145, 786
MwoI GCNNNNNNNGC 1 cut(s) 617
NciI CCSGG 1 cut(s) 180
NdeII GATC 5 cut(s) 231, 277, 536, 919, 1012
NlaIII CATG 3 cut(s) 232, 701, 942
NlaIV GGNNCC 1 cut(s) 279
NsiI ATGCAT 1 cut(s) 814
NspI RCATGY 1 cut(s) 942
PceI AGGCCT 1 cut(s) 249
PdmI GAANNNNTTC 1 cut(s) 220
PfeI GAWTC 4 cut(s) 98, 201, 373, 866
PfoI TCCNGGA 1 cut(s) 178
PkrI GCNGC 1 cut(s) 666
PleI GAGTC 2 cut(s) 104, 825
PpsI GAGTC 2 cut(s) 104, 825
Psp6I CCWGG 2 cut(s) 143, 784
PspGI CCWGG 2 cut(s) 143, 784
PspN4I GGNNCC 1 cut(s) 279
PspPI GGNCC 1 cut(s) 694
PstI CTGCAG 1 cut(s) 331
PstNI CAGNNNCTG 1 cut(s) 335
PsuI RGATCY 1 cut(s) 277
RsaI GTAC 3 cut(s) 264, 398, 410
RsaNI GTAC 3 cut(s) 263, 397, 409
RseI CAYNNNNRTG 1 cut(s) 937
SaqAI TTAA 1 cut(s) 749
SatI GCNGC 1 cut(s) 665
Sau3AI GATC 5 cut(s) 231, 277, 536, 919, 1012
Sau96I GGNCC 1 cut(s) 694
ScaI AGTACT 1 cut(s) 398
SchI GAGTC 2 cut(s) 104, 825
ScrFI CCNGG 3 cut(s) 145, 180, 786
SetI ASST 8 cut(s) 70, 163, 244, 268, 430, 783, 822, 899
SfaNI GCATC 3 cut(s) 799, 937, 961
SfcI CTRYAG 1 cut(s) 327
SinI GGWCC 1 cut(s) 694
SmiMI CAYNNNNRTG 1 cut(s) 937
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
SpeI ACTAGT 1 cut(s) 43
Sse9I AATT 3 cut(s) 138, 216, 419
SseBI AGGCCT 1 cut(s) 249
SsiI CCGC 1 cut(s) 665
SspMI CTAG 4 cut(s) 32, 44, 245, 506
StuI AGGCCT 1 cut(s) 249
StyD4I CCNGG 3 cut(s) 143, 178, 784
StyI CCWWGG 1 cut(s) 20
TaaI ACNGT 2 cut(s) 262, 550
TaiI ACGT 1 cut(s) 822
TaqI TCGA 1 cut(s) 1000
TasI AATT 3 cut(s) 138, 216, 419
TatI WGTACW 1 cut(s) 396
TauI GCSGC 1 cut(s) 667
TfiI GAWTC 4 cut(s) 98, 201, 373, 866
Tru1I TTAA 1 cut(s) 749
Tru9I TTAA 1 cut(s) 749
TspDTI ATGAA 3 cut(s) 118, 559, 968
VpaK11BI GGWCC 1 cut(s) 694
XapI RAATTY 1 cut(s) 216
XceI RCATGY 1 cut(s) 942
XcmI CCANNNNNNNNNTGG 1 cut(s) 685
XmnI GAANNNNTTC 1 cut(s) 220
XspI CTAG 4 cut(s) 32, 44, 245, 506
ZrmI AGTACT 1 cut(s) 398
Zsp2I ATGCAT 1 cut(s) 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.