Rorug02G0431200

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0013813
Physical Location & Seq
Reverse (-)
55288774 .. 55290764
1991 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0431200.1

Sequence Viewer

Length: 948 bp
ATGGAAATCGAAGTTGAAGACCGCTCAAAACTTGTAGTGGAGAAAGGTCAAAAGGCCGTGTTTGGAAGAGGAAATGGGTTCAACACCACAGACCGAACAGTTTCTCGCCGTCATGTTTTGTTCGAACTCCAGCACATAAACGCGACCCAAACAGAGCCCAGAATCTCATTCGAGGTTCTGGGGAGGAACCCCGTCTGGGTCCGGAGCCAGAAAGACGGGGAAGTCAGGGTTTTCAGAAGGTCGGAGAAGGGTGAGCTGGCCGCCGGTGATTGGCTCTGCGTGTCTGGGAAGAGACCCATTTGGTTTAAAGTGAGAAATGTTGGGGTTGAAGAGGAAGTTAAGAGGGTTTTGGAGAGTGAGAGTGAAATGGCTGAGGGTTTGCTAAATGGGTCTGAATGGGAAGGCGTTGATGTTTCAGGCATTGACCCTGTTAAAGAGTTTGGTTTCGTTGTAATGGGGAATGAGTTTGATCAGTATCCCAAGCAAATGATTTGCGATGTAAAGAACTGGAACTGGTTTCTTGAGGAACCTAGAAAAGAAAGCGACGATGATGAATATGAGGGAAAGGAAAGGAGGGGTGGGAGGAGAAAGAGGAAAAAAGGTGAAGAAAACGATAATGAAGAGTGGACAGGGGATGGTGAAGCTGATGAAGAGCTTGTTGCAAAACTTAGAAAGGTAAATAAGCCAAAATATTCTACAAGATCGAAGGCCCATAGTGAACCTCAGAAGAAAGCAAAAGGTAGTAAACATTCTGATCAAAAGAAGACGGCTGCTGTGGATGAAGAGGGCATAAAAGACGATGACAAAGATGAAGACGAAGATGATGAAACACTTGGAGGCTTCATTGTCGGTGAGGACGACTTGGAAGAAGAAGAAGACCCTGAAGGAACTGATGAATATGAAGAAGAGGAATTTGTTGAAGAAGAAGATGATGATGATGATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

315

Amino Acids

36.27

Weight (kDa)

4.56

Isoelectric Point (pI)

52.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013813)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42990
fragaria_vesca FvH4_6g38660 FvH4_6g38660
malus_domestica MD09G1148500.v1.1
prunus_persica Prupe.3G177600_v2.0.a1
pyrus_communis pycom09g06820
rosa_chinensis RchiOBHm_Chr2g0152121
rosa_laevigata RLG00000020610
rosa_multiflora Rmu_sc0000760.1_g000001 Rmu_sc0003043.1_g000023
rosa_roxburghii Rroxscaffold_2G00095910
rosa_rugosa Rorug02G0431200
rosa_samantha Rh2AG492000 Rh2BG504500 Rh2CG478500 Rh2DG515800
rosa_wichuraiana Rw2G040440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 221
AccBSI CCGCTC 1 cut(s) 24
AccII CGCG 1 cut(s) 143
AccIII TCCGGA 1 cut(s) 201
AciI CCGC 2 cut(s) 22, 261
AcoI YGGCCR 1 cut(s) 258
AcsI RAATTY 1 cut(s) 911
AcuI CTGAAG 1 cut(s) 903
AfiI CCNNNNNNNGG 2 cut(s) 196, 270
AgsI TTSAA 4 cut(s) 17, 82, 329, 920
AluBI AGCT 3 cut(s) 256, 644, 655
AluI AGCT 3 cut(s) 256, 644, 655
Alw26I GTCTC 1 cut(s) 286
Aor13HI TCCGGA 1 cut(s) 201
AoxI GGCC 3 cut(s) 54, 258, 708
ApeKI GCWGC 1 cut(s) 770
ApoI RAATTY 1 cut(s) 911
Asp700I GAANNNNTTC 1 cut(s) 100
AspS9I GGNCC 2 cut(s) 199, 709
AsuHPI GGTGA 5 cut(s) 263, 278, 614, 650, 863
AsuII TTCGAA 1 cut(s) 123
AvaII GGWCC 1 cut(s) 199
BanII GRGCYC 1 cut(s) 159
BbsI GAAGAC 4 cut(s) 24, 770, 819, 882
BbvCI CCTCAGC 1 cut(s) 372
BbvI GCAGC 1 cut(s) 757
BccI CCATC 1 cut(s) 629
BceAI ACGGC 3 cut(s) 41, 93, 783
BciVI GTATCC 1 cut(s) 486
BclI TGATCA 2 cut(s) 469, 754
BcoDI GTCTC 1 cut(s) 286
BfaI CTAG 1 cut(s) 531
BfuI GTATCC 1 cut(s) 486
BisI GCNGC 2 cut(s) 261, 771
BlsI GCNGC 2 cut(s) 262, 772
Bme18I GGWCC 1 cut(s) 199
BmgT120I GGNCC 2 cut(s) 199, 709
BmiI GGNNCC 4 cut(s) 188, 200, 206, 528
BpiI GAAGAC 4 cut(s) 24, 770, 819, 882
BpmI CTGGAG 1 cut(s) 113
Bpu10I CCTNAGC 1 cut(s) 372
Bpu14I TTCGAA 1 cut(s) 123
BpuEI CTTGAG 1 cut(s) 542
BsaBI GATNNNNATC 1 cut(s) 474
BsaI GGTCTC 1 cut(s) 286
BsaWI WCCGGW 1 cut(s) 201
Bsc4I CCNNNNNNNGG 2 cut(s) 196, 270
Bse118I RCCGGY 1 cut(s) 263
Bse1I ACTGG 2 cut(s) 512, 518
Bse8I GATNNNNATC 1 cut(s) 474
BseAI TCCGGA 1 cut(s) 201
BseGI GGATG 2 cut(s) 640, 784
BseJI GATNNNNATC 1 cut(s) 474
BseLI CCNNNNNNNGG 2 cut(s) 196, 270
BseMII CTCAG 2 cut(s) 363, 737
BseNI ACTGG 2 cut(s) 512, 518
BseRI GAGGAG 1 cut(s) 598
BseXI GCAGC 1 cut(s) 757
Bsh1236I CGCG 1 cut(s) 143
BshFI GGCC 3 cut(s) 56, 260, 710
BsiSI CCGG 2 cut(s) 202, 264
BslI CCNNNNNNNGG 2 cut(s) 196, 270
BsmAI GTCTC 1 cut(s) 286
BsnI GGCC 3 cut(s) 56, 260, 710
Bso31I GGTCTC 1 cut(s) 286
Bsp119I TTCGAA 1 cut(s) 123
Bsp1286I GDGCHC 1 cut(s) 159
Bsp13I TCCGGA 1 cut(s) 201
Bsp143I GATC 3 cut(s) 469, 701, 754
BspACI CCGC 2 cut(s) 22, 261
BspANI GGCC 3 cut(s) 56, 260, 710
BspCNI CTCAG 2 cut(s) 364, 736
BspEI TCCGGA 1 cut(s) 201
BspFNI CGCG 1 cut(s) 143
BspLI GGNNCC 4 cut(s) 188, 200, 206, 528
BspQI GCTCTTC 1 cut(s) 645
BspT104I TTCGAA 1 cut(s) 123
BspTNI GGTCTC 1 cut(s) 286
BsrBI CCGCTC 1 cut(s) 24
BsrFI RCCGGY 1 cut(s) 263
BsrI ACTGG 2 cut(s) 512, 518
BssAI RCCGGY 1 cut(s) 263
BssMI GATC 3 cut(s) 469, 701, 754
Bst4CI ACNGT 1 cut(s) 100
Bst6I CTCTTC 7 cut(s) 61, 284, 324, 615, 645, 777, 900
BstBI TTCGAA 1 cut(s) 123
BstC8I GCNNGC 1 cut(s) 258
BstDEI CTNAG 3 cut(s) 372, 668, 723
BstF5I GGATG 2 cut(s) 640, 784
BstFNI CGCG 1 cut(s) 143
BstKTI GATC 3 cut(s) 472, 704, 757
BstMAI GTCTC 1 cut(s) 286
BstMBI GATC 3 cut(s) 469, 701, 754
BstUI CGCG 1 cut(s) 143
BstV1I GCAGC 1 cut(s) 757
BstV2I GAAGAC 4 cut(s) 24, 770, 819, 882
BsuI GTATCC 1 cut(s) 486
BsuRI GGCC 3 cut(s) 56, 260, 710
BtgZI GCGATG 1 cut(s) 510
BtsCI GGATG 2 cut(s) 640, 784
Cac8I GCNNGC 1 cut(s) 258
Cfr10I RCCGGY 1 cut(s) 263
Cfr13I GGNCC 2 cut(s) 199, 709
CviAII CATG 1 cut(s) 113
DdeI CTNAG 3 cut(s) 372, 668, 723
DpnI GATC 3 cut(s) 471, 703, 756
DpnII GATC 3 cut(s) 469, 701, 754
DraI TTTAAA 1 cut(s) 307
DrdI GACNNNNNNGTC 1 cut(s) 221
DseDI GACNNNNNNGTC 1 cut(s) 221
EaeI YGGCCR 1 cut(s) 258
Eam1104I CTCTTC 7 cut(s) 61, 284, 324, 615, 645, 777, 900
EarI CTCTTC 7 cut(s) 61, 284, 324, 615, 645, 777, 900
Eco24I GRGCYC 1 cut(s) 159
Eco31I GGTCTC 1 cut(s) 286
Eco47I GGWCC 1 cut(s) 199
Eco57I CTGAAG 1 cut(s) 903
EcoT38I GRGCYC 1 cut(s) 159
FaeI CATG 1 cut(s) 116
FaiI YATR 6 cut(s) 114, 137, 558, 714, 791, 900
FatI CATG 1 cut(s) 112
FbaI TGATCA 2 cut(s) 469, 754
Fnu4HI GCNGC 2 cut(s) 261, 771
FokI GGATG 2 cut(s) 647, 791
FriOI GRGCYC 1 cut(s) 159
Fsp4HI GCNGC 2 cut(s) 261, 771
FspBI CTAG 1 cut(s) 531
GluI GCNGC 2 cut(s) 261, 771
GsuI CTGGAG 1 cut(s) 113
HaeIII GGCC 3 cut(s) 56, 260, 710
HapII CCGG 2 cut(s) 202, 264
Hin1II CATG 1 cut(s) 116
HinfI GANTC 1 cut(s) 162
HpaII CCGG 2 cut(s) 202, 264
HphI GGTGA 5 cut(s) 263, 278, 614, 650, 863
Hpy166II GTNNAC 3 cut(s) 627, 719, 746
Hpy188I TCNGA 5 cut(s) 236, 244, 394, 726, 754
Hpy188III TCNNGA 2 cut(s) 202, 521
Hpy8I GTNNAC 3 cut(s) 627, 719, 746
Hpy99I CGWCG 1 cut(s) 548
HpyAV CCTTC 5 cut(s) 231, 241, 395, 700, 878
HpyCH4III ACNGT 1 cut(s) 100
HpyCH4V TGCA 1 cut(s) 662
HpyF3I CTNAG 3 cut(s) 372, 668, 723
Hsp92II CATG 1 cut(s) 116
Kpn2I TCCGGA 1 cut(s) 201
Ksp22I TGATCA 2 cut(s) 469, 754
Kzo9I GATC 3 cut(s) 469, 701, 754
LguI GCTCTTC 1 cut(s) 645
LmnI GCTCC 1 cut(s) 204
Lsp1109I GCAGC 1 cut(s) 757
MaeI CTAG 1 cut(s) 531
MalI GATC 3 cut(s) 471, 703, 756
MbiI CCGCTC 1 cut(s) 24
MboI GATC 3 cut(s) 469, 701, 754
MhlI GDGCHC 1 cut(s) 159
MluCI AATT 1 cut(s) 911
MmeI TCCRAC 1 cut(s) 222
MroI TCCGGA 1 cut(s) 201
MroXI GAANNNNTTC 1 cut(s) 100
MseI TTAA 3 cut(s) 306, 339, 432
MspI CCGG 2 cut(s) 202, 264
MvnI CGCG 1 cut(s) 143
NdeII GATC 3 cut(s) 469, 701, 754
NlaIII CATG 1 cut(s) 116
NlaIV GGNNCC 4 cut(s) 188, 200, 206, 528
NspV TTCGAA 1 cut(s) 123
PciSI GCTCTTC 1 cut(s) 645
PcsI WCGNNNNNNNCGW 1 cut(s) 855
PdmI GAANNNNTTC 1 cut(s) 100
PfeI GAWTC 1 cut(s) 162
PkrI GCNGC 2 cut(s) 262, 772
PspN4I GGNNCC 4 cut(s) 188, 200, 206, 528
PspPI GGNCC 2 cut(s) 199, 709
SapI GCTCTTC 1 cut(s) 645
SaqAI TTAA 3 cut(s) 306, 339, 432
SatI GCNGC 2 cut(s) 261, 771
Sau3AI GATC 3 cut(s) 469, 701, 754
Sau96I GGNCC 2 cut(s) 199, 709
SduI GDGCHC 1 cut(s) 159
SfuI TTCGAA 1 cut(s) 123
SgrAI CRCCGGYG 1 cut(s) 263
SinI GGWCC 1 cut(s) 199
SmlI CTYRAG 1 cut(s) 521
SmoI CTYRAG 1 cut(s) 521
Sse9I AATT 1 cut(s) 911
SsiI CCGC 2 cut(s) 22, 261
SspI AATATT 1 cut(s) 692
SspMI CTAG 1 cut(s) 531
TaaI ACNGT 1 cut(s) 100
TaqI TCGA 4 cut(s) 9, 123, 171, 704
TaqII GACCGA 1 cut(s) 108
TasI AATT 1 cut(s) 911
TauI GCSGC 1 cut(s) 263
TfiI GAWTC 1 cut(s) 162
Tru1I TTAA 3 cut(s) 306, 339, 432
Tru9I TTAA 3 cut(s) 306, 339, 432
TseI GCWGC 1 cut(s) 770
TspDTI ATGAA 9 cut(s) 567, 633, 663, 795, 825, 832, 840, 909, 915
VpaK11BI GGWCC 1 cut(s) 199
XapI RAATTY 1 cut(s) 911
XmnI GAANNNNTTC 1 cut(s) 100
XspI CTAG 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.