AT2G43465

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0005025
Physical Location & Seq
Reverse (-)
18047533 .. 18049794
2262 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G43465.6

Sequence Viewer

Length: 1167 bp
ATGATTAAAATTAGAGATTGTCTAGATGAAATGGTGAAGTTCACACTCGATTACTGTGTAGAATTCGATATAGGGCTTACCGGGGAGTTCTGCTCCGGCCTTCTGTGCGGCGAATCAGTTCTTCACGACGGTGAACGAATCGAATCGTCTTCCTACGCTTTGCTACACCGGTTTTCAGGTGTTCCTGATTATCCCTTGTACAAGGTTCTAGCTTTAGGTCTGCTGAAATCAATTGATTCTGGTTCTTTCTGTGGAACCTTTGAGAAAATTTCGTTGGGGAAGGAAGTAATCCGGTTGAAGGAGAAAGAAGATGAGTGGAGCAAGCTGATAAATCAAAAGGGTTCTGAGTTAGTCAATGCCTTGAAGGATGTATTTTCCGAGCTTCAAGTTCAAGAGCCCTTATTCTCCCTCATGAAAGATGGCATCAAAACAGTTGAAGCAAGGTGTTTTGAAGAAGAGTATGATAGAATTCGGAGAGGAGGTTCAATGGTTATGATAAATAAATGTCTAATGTTTGAAGTTCTGGAACTTCACCAATATGCATCCTTTTACGAGCTGTTGAAAGCCGAGAGTTCAGAGAAAGTGTTTCCTGGCACTAAAACAGTGGAAGAAGGTATGCAAATGTTTAGGAAGTTATATGATACGGATCAAGAGAATTTCAATGGTGTTGTGGCAATCCATCTTAGTAAATCAGTTGCTCAGCCTTGTGTTGCTTTGGCTCACATACTGTCTGGCTTGAGTTACACTGGCGTGCAAAACCTTCTCGGTCTATCTCACACAACTGGATCCATTTTTCATGCTCTGCCTCCTCCTAGATCGATGCTACTCTCTTCATTCATGCTTCCATATAAGCCAAAGATAAAAGGTTGTAGATTGAGCCATGGAGCTAGAGCATTAGCCAAGCATGTTGATCGAAGTAGCGATGGATTTTGGGGTGTTCTTCAGGGAACTGATTCAGATAAAAATGAGCGTGCAATGGACATAATCAATCGGTTCATTGGCAATTGTTGTTGGATGAACATACATATCGTTCCACCACATGGTGAAGTGTTCGAGATAAGAGTAGCTCAAGGCTATGGAGCACGTTGGTCTCGAGATGGAACCAAATTCATTGGATTTCTTGAGCCTTACATGGAGGATGGTCACTCCATGGCCTGGAAGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ASCH

Protein Analysis

388

Amino Acids

43.75

Weight (kDa)

6.03

Isoelectric Point (pI)

34.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ASCH PF04266 128 - 229 1.3e-06 ASCH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 1040, 1155
AciI CCGC 1 cut(s) 108
AclWI GGATC 3 cut(s) 654, 780, 793
AcsI RAATTY 5 cut(s) 62, 267, 468, 655, 1106
AcuI CTGAAG 1 cut(s) 926
AdeI CACNNNGTG 1 cut(s) 1043
AfaI GTAC 1 cut(s) 200
AfiI CCNNNNNNNGG 3 cut(s) 298, 1040, 1155
AgeI ACCGGT 1 cut(s) 168
AjnI CCWGG 2 cut(s) 589, 1154
AjuI GAANNNNNNNTTGG 2 cut(s) 257, 289
AleI CACNNNNGTG 2 cut(s) 129, 749
AloI GAACNNNNNNTCC 2 cut(s) 466, 498
AluBI AGCT 6 cut(s) 212, 325, 382, 556, 887, 1067
AluI AGCT 6 cut(s) 212, 325, 382, 556, 887, 1067
Alw21I GWGCWC 1 cut(s) 1084
Alw26I GTCTC 1 cut(s) 1095
AlwI GGATC 3 cut(s) 654, 780, 793
Ama87I CYCGRG 1 cut(s) 1092
AoxI GGCC 2 cut(s) 97, 1152
ApoI RAATTY 5 cut(s) 62, 267, 468, 655, 1106
AsiGI ACCGGT 1 cut(s) 168
Asp700I GAANNNNTTC 2 cut(s) 117, 952
AsuC2I CCSGG 1 cut(s) 82
AsuHPI GGTGA 4 cut(s) 46, 143, 524, 1055
AvaI CYCGRG 1 cut(s) 1092
BamHI GGATCC 1 cut(s) 785
BanII GRGCYC 1 cut(s) 399
BbsI GAAGAC 1 cut(s) 141
Bbv12I GWGCWC 1 cut(s) 1084
BccI CCATC 5 cut(s) 413, 687, 917, 1091, 1133
BcgI CGANNNNNNTGC 2 cut(s) 893, 927
BciT130I CCWGG 2 cut(s) 591, 1156
BcnI CCSGG 1 cut(s) 82
BcoDI GTCTC 1 cut(s) 1095
BfaI CTAG 4 cut(s) 23, 209, 813, 888
BisI GCNGC 1 cut(s) 109
BlpI GCTNAGC 1 cut(s) 699
BlsI GCNGC 1 cut(s) 110
Bme1390I CCNGG 3 cut(s) 82, 591, 1156
BmeT110I CYCGRG 1 cut(s) 1092
BmiI GGNNCC 3 cut(s) 256, 787, 1102
BmrFI CCNGG 3 cut(s) 82, 591, 1156
BmsI GCATC 3 cut(s) 432, 551, 810
BpiI GAAGAC 1 cut(s) 141
BplI GAGNNNNNCTC 2 cut(s) 77, 109
Bpu1102I GCTNAGC 1 cut(s) 699
BpuEI CTTGAG 3 cut(s) 757, 1053, 1142
BpuMI CCSGG 1 cut(s) 82
Bsa29I ATCGAT 1 cut(s) 818
BsaBI GATNNNNATC 1 cut(s) 645
BsaI GGTCTC 1 cut(s) 1095
BsaJI CCNNGG 3 cut(s) 81, 880, 1149
BsaWI WCCGGW 2 cut(s) 168, 291
BsaXI ACNNNNNCTCC 2 cut(s) 466, 496
Bsc4I CCNNNNNNNGG 3 cut(s) 298, 1040, 1155
Bse118I RCCGGY 1 cut(s) 168
Bse1I ACTGG 2 cut(s) 751, 787
Bse3DI GCAATG 1 cut(s) 981
Bse8I GATNNNNATC 1 cut(s) 645
BseBI CCWGG 2 cut(s) 591, 1156
BseCI ATCGAT 1 cut(s) 818
BseDI CCNNGG 3 cut(s) 81, 880, 1149
BseGI GGATG 4 cut(s) 373, 542, 1020, 1144
BseJI GATNNNNATC 1 cut(s) 645
BseLI CCNNNNNNNGG 3 cut(s) 298, 1040, 1155
BseMI GCAATG 1 cut(s) 981
BseMII CTCAG 2 cut(s) 336, 713
BseNI ACTGG 2 cut(s) 751, 787
BseRI GAGGAG 2 cut(s) 492, 798
BshFI GGCC 2 cut(s) 99, 1154
BshTI ACCGGT 1 cut(s) 168
BshVI ATCGAT 1 cut(s) 818
BsiHKAI GWGCWC 1 cut(s) 1084
BsiHKCI CYCGRG 1 cut(s) 1092
BsiSI CCGG 4 cut(s) 81, 96, 169, 292
BslI CCNNNNNNNGG 3 cut(s) 298, 1040, 1155
BsmAI GTCTC 1 cut(s) 1095
BsnI GGCC 2 cut(s) 99, 1154
Bso31I GGTCTC 1 cut(s) 1095
BsoBI CYCGRG 1 cut(s) 1092
Bsp1286I GDGCHC 2 cut(s) 399, 1084
Bsp1407I TGTACA 1 cut(s) 198
Bsp143I GATC 4 cut(s) 646, 785, 815, 910
Bsp1720I GCTNAGC 1 cut(s) 699
Bsp19I CCATGG 2 cut(s) 880, 1149
BspACI CCGC 1 cut(s) 108
BspANI GGCC 2 cut(s) 99, 1154
BspCNI CTCAG 2 cut(s) 337, 712
BspDI ATCGAT 1 cut(s) 818
BspHI TCATGA 1 cut(s) 411
BspLI GGNNCC 3 cut(s) 256, 787, 1102
BspPI GGATC 3 cut(s) 654, 780, 793
BspTNI GGTCTC 1 cut(s) 1095
BsrDI GCAATG 1 cut(s) 981
BsrFI RCCGGY 1 cut(s) 168
BsrGI TGTACA 1 cut(s) 198
BsrI ACTGG 2 cut(s) 751, 787
BssAI RCCGGY 1 cut(s) 168
BssECI CCNNGG 3 cut(s) 81, 880, 1149
BssMI GATC 4 cut(s) 646, 785, 815, 910
BssT1I CCWWGG 2 cut(s) 880, 1149
Bst2UI CCWGG 2 cut(s) 591, 1156
Bst4CI ACNGT 5 cut(s) 56, 131, 433, 604, 729
Bst6I CTCTTC 2 cut(s) 450, 835
BstAUI TGTACA 1 cut(s) 198
BstC8I GCNNGC 3 cut(s) 323, 752, 972
BstDEI CTNAG 3 cut(s) 345, 683, 699
BstDSI CCRYGG 2 cut(s) 880, 1149
BstF5I GGATG 4 cut(s) 373, 542, 1020, 1144
BstKTI GATC 4 cut(s) 649, 788, 818, 913
BstMAI GTCTC 1 cut(s) 1095
BstMBI GATC 4 cut(s) 646, 785, 815, 910
BstMWI GCNNNNNNNGC 1 cut(s) 105
BstNI CCWGG 2 cut(s) 591, 1156
BstNSI RCATGY 1 cut(s) 908
BstSCI CCNGG 3 cut(s) 80, 589, 1154
BstV2I GAAGAC 1 cut(s) 141
BstX2I RGATCY 1 cut(s) 785
BstYI RGATCY 1 cut(s) 785
Bsu15I ATCGAT 1 cut(s) 818
BsuRI GGCC 2 cut(s) 99, 1154
BsuTUI ATCGAT 1 cut(s) 818
BtgI CCRYGG 2 cut(s) 880, 1149
BtgZI GCGATG 1 cut(s) 936
BtsCI GGATG 4 cut(s) 373, 542, 1020, 1144
BtsIMutI CAGTG 2 cut(s) 609, 744
Cac8I GCNNGC 3 cut(s) 323, 752, 972
CciI TCATGA 1 cut(s) 411
Cfr10I RCCGGY 1 cut(s) 168
ClaI ATCGAT 1 cut(s) 818
Csp6I GTAC 1 cut(s) 199
CspAI ACCGGT 1 cut(s) 168
CviAII CATG 8 cut(s) 412, 797, 838, 881, 905, 1040, 1132, 1150
CviQI GTAC 1 cut(s) 199
DdeI CTNAG 3 cut(s) 345, 683, 699
DpnI GATC 4 cut(s) 648, 787, 817, 912
DpnII GATC 4 cut(s) 646, 785, 815, 910
DraIII CACNNNGTG 1 cut(s) 1043
Eam1104I CTCTTC 2 cut(s) 450, 835
EarI CTCTTC 2 cut(s) 450, 835
Eco130I CCWWGG 2 cut(s) 880, 1149
Eco24I GRGCYC 1 cut(s) 399
Eco31I GGTCTC 1 cut(s) 1095
Eco57I CTGAAG 1 cut(s) 926
Eco88I CYCGRG 1 cut(s) 1092
EcoRI GAATTC 2 cut(s) 62, 468
EcoRII CCWGG 2 cut(s) 589, 1154
EcoT14I CCWWGG 2 cut(s) 880, 1149
EcoT22I ATGCAT 1 cut(s) 544
EcoT38I GRGCYC 1 cut(s) 399
ErhI CCWWGG 2 cut(s) 880, 1149
FaeI CATG 8 cut(s) 415, 800, 841, 884, 908, 1043, 1135, 1153
FatI CATG 8 cut(s) 411, 796, 837, 880, 904, 1039, 1131, 1149
Fnu4HI GCNGC 1 cut(s) 109
FokI GGATG 4 cut(s) 380, 529, 1027, 1151
FriOI GRGCYC 1 cut(s) 399
Fsp4HI GCNGC 1 cut(s) 109
FspBI CTAG 4 cut(s) 23, 209, 813, 888
GluI GCNGC 1 cut(s) 109
HaeIII GGCC 2 cut(s) 99, 1154
HapII CCGG 4 cut(s) 81, 96, 169, 292
Hin1II CATG 8 cut(s) 415, 800, 841, 884, 908, 1043, 1135, 1153
HinfI GANTC 5 cut(s) 113, 138, 143, 236, 953
HpaII CCGG 4 cut(s) 81, 96, 169, 292
HphI GGTGA 4 cut(s) 46, 143, 524, 1055
Hpy166II GTNNAC 2 cut(s) 42, 134
Hpy188I TCNGA 5 cut(s) 346, 379, 474, 577, 958
Hpy8I GTNNAC 2 cut(s) 42, 134
Hpy99I CGWCG 1 cut(s) 131
HpyAV CCTTC 6 cut(s) 110, 274, 292, 358, 605, 770
HpyCH4III ACNGT 5 cut(s) 56, 131, 433, 604, 729
HpyCH4IV ACGT 1 cut(s) 1084
HpyCH4V TGCA 4 cut(s) 542, 619, 754, 974
HpyF10VI GCNNNNNNNGC 1 cut(s) 105
HpyF3I CTNAG 3 cut(s) 345, 683, 699
HpySE526I ACGT 1 cut(s) 1084
Hsp92II CATG 8 cut(s) 415, 800, 841, 884, 908, 1043, 1135, 1153
Kzo9I GATC 4 cut(s) 646, 785, 815, 910
LmnI GCTCC 4 cut(s) 98, 318, 884, 1079
LweI GCATC 3 cut(s) 432, 551, 810
MaeI CTAG 4 cut(s) 23, 209, 813, 888
MaeII ACGT 1 cut(s) 1084
MaeIII GTNAC 2 cut(s) 740, 1142
MalI GATC 4 cut(s) 648, 787, 817, 912
MboI GATC 4 cut(s) 646, 785, 815, 910
MboII GAAGA 8 cut(s) 113, 141, 320, 464, 467, 620, 822, 932
MfeI CAATTG 2 cut(s) 231, 1003
MflI RGATCY 1 cut(s) 785
MhlI GDGCHC 2 cut(s) 399, 1084
MluCI AATT 8 cut(s) 9, 62, 231, 267, 468, 655, 1003, 1106
MmeI TCCRAC 1 cut(s) 992
MnlI CCTC 6 cut(s) 419, 470, 473, 816, 819, 1129
Mph1103I ATGCAT 1 cut(s) 544
MroXI GAANNNNTTC 2 cut(s) 117, 952
MseI TTAA 2 cut(s) 6, 1165
MslI CAYNNNNRTG 3 cut(s) 129, 537, 749
MspI CCGG 4 cut(s) 81, 96, 169, 292
MspR9I CCNGG 3 cut(s) 82, 591, 1156
MunI CAATTG 2 cut(s) 231, 1003
MvaI CCWGG 2 cut(s) 591, 1156
MwoI GCNNNNNNNGC 1 cut(s) 105
NciI CCSGG 1 cut(s) 82
NcoI CCATGG 2 cut(s) 880, 1149
NdeII GATC 4 cut(s) 646, 785, 815, 910
NlaIII CATG 8 cut(s) 415, 800, 841, 884, 908, 1043, 1135, 1153
NlaIV GGNNCC 3 cut(s) 256, 787, 1102
NmeAIII GCCGAG 1 cut(s) 592
NmuCI GTSAC 1 cut(s) 1142
NsiI ATGCAT 1 cut(s) 544
NspI RCATGY 1 cut(s) 908
OliI CACNNNNGTG 2 cut(s) 129, 749
PaeR7I CTCGAG 1 cut(s) 1092
PagI TCATGA 1 cut(s) 411
PcsI WCGNNNNNNNCGW 1 cut(s) 1090
PdmI GAANNNNTTC 2 cut(s) 117, 952
PfeI GAWTC 5 cut(s) 113, 138, 143, 236, 953
PflMI CCANNNNNTGG 2 cut(s) 1040, 1155
PinAI ACCGGT 1 cut(s) 168
PkrI GCNGC 1 cut(s) 110
Psp6I CCWGG 2 cut(s) 589, 1154
PspGI CCWGG 2 cut(s) 589, 1154
PspN4I GGNNCC 3 cut(s) 256, 787, 1102
PsuI RGATCY 1 cut(s) 785
RsaI GTAC 1 cut(s) 200
RsaNI GTAC 1 cut(s) 199
RseI CAYNNNNRTG 3 cut(s) 129, 537, 749
SaqAI TTAA 2 cut(s) 6, 1165
SatI GCNGC 1 cut(s) 109
Sau3AI GATC 4 cut(s) 646, 785, 815, 910
ScrFI CCNGG 3 cut(s) 82, 591, 1156
SduI GDGCHC 2 cut(s) 399, 1084
SfaNI GCATC 3 cut(s) 432, 551, 810
Sfr274I CTCGAG 1 cut(s) 1092
SlaI CTCGAG 1 cut(s) 1092
SmiMI CAYNNNNRTG 3 cut(s) 129, 537, 749
SmlI CTYRAG 4 cut(s) 736, 1068, 1092, 1121
SmoI CTYRAG 4 cut(s) 736, 1068, 1092, 1121
Sse9I AATT 8 cut(s) 9, 62, 231, 267, 468, 655, 1003, 1106
SsiI CCGC 1 cut(s) 108
SspMI CTAG 4 cut(s) 23, 209, 813, 888
StyD4I CCNGG 3 cut(s) 80, 589, 1154
StyI CCWWGG 2 cut(s) 880, 1149
TaaI ACNGT 5 cut(s) 56, 131, 433, 604, 729
TaiI ACGT 1 cut(s) 1087
TaqI TCGA 7 cut(s) 48, 66, 141, 818, 913, 1053, 1093
TaqII GACCGA 1 cut(s) 755
TasI AATT 8 cut(s) 9, 62, 231, 267, 468, 655, 1003, 1106
TatI WGTACW 1 cut(s) 198
TauI GCSGC 1 cut(s) 111
TfiI GAWTC 5 cut(s) 113, 138, 143, 236, 953
Tru1I TTAA 2 cut(s) 6, 1165
Tru9I TTAA 2 cut(s) 6, 1165
TscAI CASTG 2 cut(s) 609, 751
TseFI GTSAC 1 cut(s) 1142
Tsp45I GTSAC 1 cut(s) 1142
TspDTI ATGAA 8 cut(s) 42, 428, 785, 822, 826, 985, 1031, 1099
TspGWI ACGGA 1 cut(s) 659
TspRI CASTG 2 cut(s) 609, 751
Van91I CCANNNNNTGG 2 cut(s) 1040, 1155
XapI RAATTY 5 cut(s) 62, 267, 468, 655, 1106
XbaI TCTAGA 1 cut(s) 22
XceI RCATGY 1 cut(s) 908
XhoI CTCGAG 1 cut(s) 1092
XmnI GAANNNNTTC 2 cut(s) 117, 952
XspI CTAG 4 cut(s) 23, 209, 813, 888
Zsp2I ATGCAT 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.