MD04G1046100.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005025
Physical Location & Seq
Reverse (-)
5351712 .. 5359691
7980 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1046100.v1.1.491

Sequence Viewer

Length: 1182 bp
ATGGCAGAGCGGGTTAACCTGGCATACACTCTCGAAGAGCTCCTCAACTTCACTCTCCAATCTTTCGTAAATGGAACCCTGGAACTCGATCTAGGGTTGCCCAAGGACTTCTGCTCTCAACTACTCCAACCCAAACCCGAACCCGAACCGAACGACGACCTCTTCCCCCCTTCCCCCTCCGATACTACAGATTGTTTTCAAGGGATTCCACCCTACCCTTTATACAAGCGTCTGGCATTGGCTTTGCACGAATCCATAATTTCTGGTACCTGTCTTGGCACACGTCACAATATGGCCTCGGATGTTCAGGAAAGTTCCTTGAAAGAAGAAAAAAATGGATGGCAGAAGCTGATATCGGAGAAGGGATCTGAATTATTCAATATGTTAAGGGCTGTTGACTTTGAGCTTCATGTTCAGGAGCCTTTCTTTTCACAGCTTAAAGATGGCCTCAAAACAATTGAAGGAAGGTGTGCTATTGGTGATTACTGTAGAATTGTCTCAGGATCTTTGATTCTCTTCAATAAATGTATACTAATTGAAGTTCAGGATGTTCGCAGTTATTCTTTGTTCTCTGATATGATGGAGGCTGAGGGTCTATCTAAAGTCCTTCCTGGTGTTGAAACCATCGAACAAGGTGTGCAAGTATACAGAAAGTTTTACACAGAGGAGAAGGAAATGTCAAATGGTGTCCTTGCGATTAGTGTTTCAAAATTGCCTCTCCAGCCTTGCGATTTATTAGCTGGCATGCTCTTTGGATTGAATAAGGGAGGTCTTCAAGGCCTTCTTGGTCTGGCACATACCACAGGGACTATTCCAGAGGTTCTTCCCCCTCCAAAATCTACTCTTCTGTCATCGTTTATGCTGCCATATAAATTGAATGTTGAACATAGCACATTGACTCATGGAGCTAGGGCCCTGGCAAAACACGCAGAGCGGAGTAGCAGTAAATATTGGGGTACTATAGATGGAAACGATTCCAATAAAAACAGGCTTGCATGGGATGTGATCAGTCGTTTAATGACACATTGTTGCTGGTTAAATGTGCATATTGTTCAACCACATGGCGCCATCTTTGAAATCAGGGTTGCTGAAGGATATGGTGCACGGTGGTCGAGTGATGGTAGTAAGTTTATTGGATTTCTAGAGCCATACATGGAGCACTCAAAGAGATGGATGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ASCH

Protein Analysis

394

Amino Acids

43.94

Weight (kDa)

5.68

Isoelectric Point (pI)

51.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ASCH PF04266 136 - 237 2.1e-12 ASCH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 266
AccB1I GGYRCC 2 cut(s) 266, 1064
AccBSI CCGCTC 2 cut(s) 10, 934
AccI GTMKAC 2 cut(s) 529, 645
AciI CCGC 2 cut(s) 10, 934
AclWI GGATC 2 cut(s) 373, 511
AcuI CTGAAG 1 cut(s) 1110
AcyI GRCGYC 1 cut(s) 1065
AfaI GTAC 2 cut(s) 268, 958
AflIII ACRYGT 1 cut(s) 281
AjiI CACGTC 1 cut(s) 284
AjnI CCWGG 4 cut(s) 18, 78, 610, 915
AluBI AGCT 6 cut(s) 40, 349, 406, 436, 740, 908
AluI AGCT 6 cut(s) 40, 349, 406, 436, 740, 908
Alw21I GWGCWC 3 cut(s) 42, 1105, 1161
Alw26I GTCTC 1 cut(s) 502
Alw44I GTGCAC 1 cut(s) 1101
AlwI GGATC 2 cut(s) 373, 511
AlwNI CAGNNNCTG 1 cut(s) 349
AoxI GGCC 4 cut(s) 294, 445, 778, 912
ApaI GGGCCC 1 cut(s) 916
ApaLI GTGCAC 1 cut(s) 1101
ApeKI GCWGC 1 cut(s) 862
Asp700I GAANNNNTTC 1 cut(s) 973
Asp718I GGTACC 1 cut(s) 266
AspLEI GCGC 1 cut(s) 1067
AspS9I GGNCC 2 cut(s) 912, 913
AsuHPI GGTGA 1 cut(s) 491
BaeGI GKGCMC 2 cut(s) 916, 1105
BanI GGYRCC 2 cut(s) 266, 1064
BanII GRGCYC 2 cut(s) 42, 916
BbsI GAAGAC 1 cut(s) 764
Bbv12I GWGCWC 3 cut(s) 42, 1105, 1161
BbvCI CCTCAGC 1 cut(s) 588
BbvI GCAGC 1 cut(s) 849
BccI CCATC 8 cut(s) 333, 437, 574, 632, 959, 1076, 1112, 1164
BcgI CGANNNNNNTGC 2 cut(s) 1092, 1126
BciT130I CCWGG 4 cut(s) 20, 80, 612, 917
BclI TGATCA 1 cut(s) 1005
BcoDI GTCTC 1 cut(s) 502
BfaI CTAG 3 cut(s) 92, 909, 1142
BfmI CTRYAG 3 cut(s) 186, 487, 960
BfoI RGCGCY 1 cut(s) 1068
BisI GCNGC 1 cut(s) 863
BlsI GCNGC 1 cut(s) 864
Bme1390I CCNGG 4 cut(s) 20, 80, 612, 917
BmgBI CACGTC 1 cut(s) 284
BmgT120I GGNCC 2 cut(s) 912, 913
BmiI GGNNCC 5 cut(s) 76, 268, 420, 914, 1066
BmrFI CCNGG 4 cut(s) 20, 80, 612, 917
BmsI GCATC 1 cut(s) 1164
BpiI GAAGAC 1 cut(s) 764
BpmI CTGGAG 1 cut(s) 704
Bpu10I CCTNAGC 1 cut(s) 588
BsaHI GRCGYC 1 cut(s) 1065
BsaJI CCNNGG 4 cut(s) 78, 102, 297, 915
BsaXI ACNNNNNCTCC 2 cut(s) 928, 958
BseBI CCWGG 4 cut(s) 20, 80, 612, 917
BseDI CCNNGG 4 cut(s) 78, 102, 297, 915
BseGI GGATG 5 cut(s) 307, 344, 553, 1006, 1179
BseMII CTCAG 2 cut(s) 513, 579
BseRI GAGGAG 2 cut(s) 32, 680
BseSI GKGCMC 2 cut(s) 916, 1105
BseXI GCAGC 1 cut(s) 849
BshFI GGCC 4 cut(s) 296, 447, 780, 914
BshNI GGYRCC 2 cut(s) 266, 1064
BsiHKAI GWGCWC 3 cut(s) 42, 1105, 1161
BslFI GGGAC 1 cut(s) 820
BsmAI GTCTC 1 cut(s) 502
BsmFI GGGAC 1 cut(s) 820
BsnI GGCC 4 cut(s) 296, 447, 780, 914
Bsp120I GGGCCC 1 cut(s) 912
Bsp1286I GDGCHC 4 cut(s) 42, 916, 1105, 1161
Bsp143I GATC 4 cut(s) 88, 365, 503, 1005
BspACI CCGC 2 cut(s) 10, 934
BspANI GGCC 4 cut(s) 296, 447, 780, 914
BspCNI CTCAG 2 cut(s) 512, 580
BspLI GGNNCC 5 cut(s) 76, 268, 420, 914, 1066
BspPI GGATC 2 cut(s) 373, 511
BspQI GCTCTTC 1 cut(s) 30
BspT107I GGYRCC 2 cut(s) 266, 1064
BsrBI CCGCTC 2 cut(s) 10, 934
BssECI CCNNGG 4 cut(s) 78, 102, 297, 915
BssMI GATC 4 cut(s) 88, 365, 503, 1005
BssNAI GTATAC 2 cut(s) 530, 646
BssNI GRCGYC 1 cut(s) 1065
BssT1I CCWWGG 1 cut(s) 102
Bst1107I GTATAC 2 cut(s) 530, 646
Bst2UI CCWGG 4 cut(s) 20, 80, 612, 917
Bst4CI ACNGT 2 cut(s) 488, 1107
Bst6I CTCTTC 4 cut(s) 30, 167, 521, 849
BstACI GRCGYC 1 cut(s) 1065
BstC8I GCNNGC 3 cut(s) 742, 746, 993
BstDEI CTNAG 2 cut(s) 499, 588
BstF5I GGATG 5 cut(s) 307, 344, 553, 1006, 1179
BstH2I RGCGCY 1 cut(s) 1068
BstHHI GCGC 1 cut(s) 1067
BstKTI GATC 4 cut(s) 91, 368, 506, 1008
BstMAI GTCTC 1 cut(s) 502
BstMBI GATC 4 cut(s) 88, 365, 503, 1005
BstMWI GCNNNNNNNGC 2 cut(s) 721, 926
BstNI CCWGG 4 cut(s) 20, 80, 612, 917
BstNSI RCATGY 1 cut(s) 748
BstSCI CCNGG 4 cut(s) 18, 78, 610, 915
BstSFI CTRYAG 3 cut(s) 186, 487, 960
BstSLI GKGCMC 2 cut(s) 916, 1105
BstV1I GCAGC 1 cut(s) 849
BstV2I GAAGAC 1 cut(s) 764
BstX2I RGATCY 2 cut(s) 365, 503
BstYI RGATCY 2 cut(s) 365, 503
BstZ17I GTATAC 2 cut(s) 530, 646
BsuRI GGCC 4 cut(s) 296, 447, 780, 914
BtrI CACGTC 1 cut(s) 284
BtsCI GGATG 5 cut(s) 307, 344, 553, 1006, 1179
Cac8I GCNNGC 3 cut(s) 742, 746, 993
CaiI CAGNNNCTG 1 cut(s) 349
CfoI GCGC 1 cut(s) 1067
Cfr13I GGNCC 2 cut(s) 912, 913
CseI GACGC 1 cut(s) 218
Csp6I GTAC 2 cut(s) 267, 957
CviAII CATG 6 cut(s) 410, 745, 902, 996, 1061, 1153
CviQI GTAC 2 cut(s) 267, 957
DdeI CTNAG 2 cut(s) 499, 588
DinI GGCGCC 1 cut(s) 1066
DpnI GATC 4 cut(s) 90, 367, 505, 1007
DpnII GATC 4 cut(s) 88, 365, 503, 1005
Eam1104I CTCTTC 4 cut(s) 30, 167, 521, 849
EarI CTCTTC 4 cut(s) 30, 167, 521, 849
Ecl136II GAGCTC 1 cut(s) 40
Eco130I CCWWGG 1 cut(s) 102
Eco147I AGGCCT 1 cut(s) 780
Eco24I GRGCYC 2 cut(s) 42, 916
Eco32I GATATC 1 cut(s) 354
Eco53kI GAGCTC 1 cut(s) 40
Eco57I CTGAAG 1 cut(s) 1110
EcoICRI GAGCTC 1 cut(s) 40
EcoO109I RGGNCCY 2 cut(s) 912, 913
EcoRII CCWGG 4 cut(s) 18, 78, 610, 915
EcoRV GATATC 1 cut(s) 354
EcoT14I CCWWGG 1 cut(s) 102
EcoT38I GRGCYC 2 cut(s) 42, 916
EgeI GGCGCC 1 cut(s) 1066
EheI GGCGCC 1 cut(s) 1066
ErhI CCWWGG 1 cut(s) 102
FaeI CATG 6 cut(s) 413, 748, 905, 999, 1064, 1156
FalI AAGNNNNNCTT 2 cut(s) 768, 800
FaqI GGGAC 1 cut(s) 820
FatI CATG 6 cut(s) 409, 744, 901, 995, 1060, 1152
FauI CCCGC 1 cut(s) 3
FbaI TGATCA 1 cut(s) 1005
FblI GTMKAC 2 cut(s) 529, 645
Fnu4HI GCNGC 1 cut(s) 863
FokI GGATG 4 cut(s) 314, 351, 560, 1013
FriOI GRGCYC 2 cut(s) 42, 916
Fsp4HI GCNGC 1 cut(s) 863
FspBI CTAG 3 cut(s) 92, 909, 1142
GlaI GCGC 1 cut(s) 1066
GluI GCNGC 1 cut(s) 863
GsuI CTGGAG 1 cut(s) 704
HaeII RGCGCY 1 cut(s) 1068
HaeIII GGCC 4 cut(s) 296, 447, 780, 914
HgaI GACGC 1 cut(s) 218
HhaI GCGC 1 cut(s) 1067
Hin1I GRCGYC 1 cut(s) 1065
Hin1II CATG 6 cut(s) 413, 748, 905, 999, 1064, 1156
Hin6I GCGC 1 cut(s) 1065
HinP1I GCGC 1 cut(s) 1065
HincII GTYRAC 2 cut(s) 16, 397
HindII GTYRAC 2 cut(s) 16, 397
HinfI GANTC 5 cut(s) 205, 251, 511, 898, 974
HpaI GTTAAC 1 cut(s) 16
HphI GGTGA 1 cut(s) 491
Hpy166II GTNNAC 5 cut(s) 16, 397, 530, 646, 1103
Hpy188I TCNGA 5 cut(s) 181, 301, 358, 370, 574
Hpy188III TCNNGA 7 cut(s) 32, 308, 416, 501, 545, 815, 1142
Hpy8I GTNNAC 5 cut(s) 16, 397, 530, 646, 1103
Hpy99I CGWCG 1 cut(s) 158
HpyAV CCTTC 8 cut(s) 180, 355, 455, 459, 617, 664, 791, 1085
HpyCH4III ACNGT 2 cut(s) 488, 1107
HpyCH4IV ACGT 1 cut(s) 283
HpyCH4V TGCA 6 cut(s) 247, 640, 995, 1045, 1103, 1177
HpyF10VI GCNNNNNNNGC 2 cut(s) 721, 926
HpyF3I CTNAG 2 cut(s) 499, 588
HpySE526I ACGT 1 cut(s) 283
Hsp92I GRCGYC 1 cut(s) 1065
Hsp92II CATG 6 cut(s) 413, 748, 905, 999, 1064, 1156
HspAI GCGC 1 cut(s) 1065
KasI GGCGCC 1 cut(s) 1064
KpnI GGTACC 1 cut(s) 270
Ksp22I TGATCA 1 cut(s) 1005
KspAI GTTAAC 1 cut(s) 16
Kzo9I GATC 4 cut(s) 88, 365, 503, 1005
LguI GCTCTTC 1 cut(s) 30
LmnI GCTCC 4 cut(s) 45, 418, 905, 1156
Lsp1109I GCAGC 1 cut(s) 849
LweI GCATC 1 cut(s) 1164
MaeI CTAG 3 cut(s) 92, 909, 1142
MaeII ACGT 1 cut(s) 283
MaeIII GTNAC 1 cut(s) 284
MalI GATC 4 cut(s) 90, 367, 505, 1007
MbiI CCGCTC 2 cut(s) 10, 934
MboI GATC 4 cut(s) 88, 365, 503, 1005
MboII GAAGA 7 cut(s) 47, 154, 338, 508, 764, 815, 836
MfeI CAATTG 1 cut(s) 456
MflI RGATCY 2 cut(s) 365, 503
MhlI GDGCHC 4 cut(s) 42, 916, 1105, 1161
MluCI AATT 7 cut(s) 258, 371, 456, 492, 534, 710, 872
Mly113I GGCGCC 1 cut(s) 1065
MlyI GAGTC 1 cut(s) 892
MmeI TCCRAC 1 cut(s) 151
MroXI GAANNNNTTC 1 cut(s) 973
MseI TTAA 5 cut(s) 15, 386, 438, 1016, 1037
MspR9I CCNGG 4 cut(s) 20, 80, 612, 917
MunI CAATTG 1 cut(s) 456
MvaI CCWGG 4 cut(s) 20, 80, 612, 917
MwoI GCNNNNNNNGC 2 cut(s) 721, 926
NarI GGCGCC 1 cut(s) 1065
NdeII GATC 4 cut(s) 88, 365, 503, 1005
NlaIII CATG 6 cut(s) 413, 748, 905, 999, 1064, 1156
NlaIV GGNNCC 5 cut(s) 76, 268, 420, 914, 1066
NmuCI GTSAC 1 cut(s) 284
NspI RCATGY 1 cut(s) 748
PaeI GCATGC 1 cut(s) 748
PceI AGGCCT 1 cut(s) 780
PciSI GCTCTTC 1 cut(s) 30
PdmI GAANNNNTTC 1 cut(s) 973
PfeI GAWTC 4 cut(s) 205, 251, 511, 974
PkrI GCNGC 1 cut(s) 864
PleI GAGTC 1 cut(s) 892
PluTI GGCGCC 1 cut(s) 1068
PpsI GAGTC 1 cut(s) 892
Psp124BI GAGCTC 1 cut(s) 42
Psp6I CCWGG 4 cut(s) 18, 78, 610, 915
PspGI CCWGG 4 cut(s) 18, 78, 610, 915
PspN4I GGNNCC 5 cut(s) 76, 268, 420, 914, 1066
PspOMI GGGCCC 1 cut(s) 912
PspPI GGNCC 2 cut(s) 912, 913
PstNI CAGNNNCTG 1 cut(s) 349
PsuI RGATCY 2 cut(s) 365, 503
RsaI GTAC 2 cut(s) 268, 958
RsaNI GTAC 2 cut(s) 267, 957
SacI GAGCTC 1 cut(s) 42
SapI GCTCTTC 1 cut(s) 30
SaqAI TTAA 5 cut(s) 15, 386, 438, 1016, 1037
SatI GCNGC 1 cut(s) 863
Sau3AI GATC 4 cut(s) 88, 365, 503, 1005
Sau96I GGNCC 2 cut(s) 912, 913
SchI GAGTC 1 cut(s) 892
ScrFI CCNGG 4 cut(s) 20, 80, 612, 917
SduI GDGCHC 4 cut(s) 42, 916, 1105, 1161
SfaNI GCATC 1 cut(s) 1164
SfcI CTRYAG 3 cut(s) 186, 487, 960
SfoI GGCGCC 1 cut(s) 1066
SphI GCATGC 1 cut(s) 748
Sse9I AATT 7 cut(s) 258, 371, 456, 492, 534, 710, 872
SseBI AGGCCT 1 cut(s) 780
SsiI CCGC 2 cut(s) 10, 934
SspDI GGCGCC 1 cut(s) 1064
SspI AATATT 1 cut(s) 950
SspMI CTAG 3 cut(s) 92, 909, 1142
SstI GAGCTC 1 cut(s) 42
StuI AGGCCT 1 cut(s) 780
StyD4I CCNGG 4 cut(s) 18, 78, 610, 915
StyI CCWWGG 1 cut(s) 102
TaaI ACNGT 2 cut(s) 488, 1107
TaiI ACGT 1 cut(s) 286
TaqI TCGA 4 cut(s) 33, 87, 627, 1112
TasI AATT 7 cut(s) 258, 371, 456, 492, 534, 710, 872
TfiI GAWTC 4 cut(s) 205, 251, 511, 974
Tru1I TTAA 5 cut(s) 15, 386, 438, 1016, 1037
Tru9I TTAA 5 cut(s) 15, 386, 438, 1016, 1037
TseFI GTSAC 1 cut(s) 284
TseI GCWGC 1 cut(s) 862
Tsp45I GTSAC 1 cut(s) 284
TspDTI ATGAA 1 cut(s) 398
VneI GTGCAC 1 cut(s) 1101
XbaI TCTAGA 1 cut(s) 1141
XceI RCATGY 1 cut(s) 748
XmiI GTMKAC 2 cut(s) 529, 645
XmnI GAANNNNTTC 1 cut(s) 973
XspI CTAG 3 cut(s) 92, 909, 1142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.