AT3G03320

ASCH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005025
Physical Location & Seq
Forward (+)
781715 .. 783464
1750 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G03320.1

Sequence Viewer

Length: 735 bp
ATGGAGCAACCAATGTCGCCGGGAACGAAGTCGGTGGATCTCCGAGAATGCATGGAATCACTTCTCCGTTTTAGTCTGAGATCTCATTTAAACGAATCTGTTCCAAGTTTCGATCTTGACCTGACCAGAGATTTTTGTCTTCATCTCCTTGGGGAAGCTACAGATTCAACAGAAAAATCTGCGGTGTACAAGCTTCTAGCAACGGCTTTGTCTGAATGTCTAGCTTCCGAGGGTGATAAAAATTCTAACTTGGAAAAATACAGCAAACTGATTCATGGCTTGGGATATGACTTAATAAATATGTTGAAGGAGGTGAACTTTGAGCTTCATGTCCAAGAGCCTTACTTCACCCAATTGAAAGATGGTTTGAAAACTGTTGAAGGAAGATGCGCAGTGGGAGACTACATGAGAATTAGTTCAGGAGATTTTCTTTTGTTCAATAAATGCTTGCTGCTTGAAGTTCAGGACGTTCACCGTTATACTTCATTCTCAGAAATGCTGAAAGTGGAGGGTCTTGCCAAAGTTCTTCCTGGAGTTGAAAGTATAGAAGAAGGTGTTCAAGTTTACCGAAACTTTTATTCTGAAGAGAAGGAAAGAATGAACGGTGTTGTAGCTATTCGTGTTGCAAAACCAGCTAATCAGCCTTCTGCAGCTTTGGCAGGAGTATTGTCTGAACTGAAGTCCAGTGGGATCAAAAGCCTGTTGGATGAATACACAGCTGGGGTTACCTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ASCH

Protein Analysis

244

Amino Acids

27.12

Weight (kDa)

5.08

Isoelectric Point (pI)

40.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ASCH PF04266 109 - 211 4.2e-14 ASCH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 391
AciI CCGC 1 cut(s) 182
AclWI GGATC 2 cut(s) 45, 698
AcsI RAATTY 1 cut(s) 241
AcuI CTGAAG 2 cut(s) 603, 698
AfaI GTAC 1 cut(s) 188
AgsI TTSAA 9 cut(s) 168, 307, 358, 370, 380, 439, 458, 539, 560
AjnI CCWGG 1 cut(s) 529
AluBI AGCT 8 cut(s) 158, 193, 224, 325, 614, 635, 653, 719
AluI AGCT 8 cut(s) 158, 193, 224, 325, 614, 635, 653, 719
Alw26I GTCTC 1 cut(s) 393
AlwI GGATC 2 cut(s) 45, 698
ApeKI GCWGC 2 cut(s) 451, 650
ApoI RAATTY 1 cut(s) 241
Asp700I GAANNNNTTC 4 cut(s) 60, 99, 415, 555
AspLEI GCGC 1 cut(s) 392
AsuC2I CCSGG 1 cut(s) 21
AsuHPI GGTGA 4 cut(s) 245, 325, 340, 464
BbsI GAAGAC 1 cut(s) 131
BbvI GCAGC 2 cut(s) 438, 662
BccI CCATC 1 cut(s) 356
BceAI ACGGC 1 cut(s) 219
BciT130I CCWGG 1 cut(s) 531
BcnI CCSGG 1 cut(s) 21
BcoDI GTCTC 1 cut(s) 393
BfaI CTAG 2 cut(s) 197, 221
BfmI CTRYAG 2 cut(s) 159, 648
BglII AGATCT 1 cut(s) 80
BisI GCNGC 2 cut(s) 452, 651
BlsI GCNGC 2 cut(s) 453, 652
Bme1390I CCNGG 2 cut(s) 21, 531
BmrFI CCNGG 2 cut(s) 21, 531
BmsI GCATC 1 cut(s) 377
BpiI GAAGAC 1 cut(s) 131
BpmI CTGGAG 1 cut(s) 552
BpuMI CCSGG 1 cut(s) 21
BsaJI CCNNGG 2 cut(s) 148, 228
Bse1I ACTGG 1 cut(s) 684
BseBI CCWGG 1 cut(s) 531
BseDI CCNNGG 2 cut(s) 148, 228
BseGI GGATG 1 cut(s) 712
BseMII CTCAG 2 cut(s) 68, 504
BseNI ACTGG 1 cut(s) 684
BseXI GCAGC 2 cut(s) 438, 662
BseYI CCCAGC 1 cut(s) 719
BsiSI CCGG 1 cut(s) 20
BsmAI GTCTC 1 cut(s) 393
BsmI GAATGC 1 cut(s) 53
Bsp1407I TGTACA 1 cut(s) 186
Bsp143I GATC 4 cut(s) 37, 80, 112, 690
BspACI CCGC 1 cut(s) 182
BspCNI CTCAG 2 cut(s) 69, 503
BspMAI CTGCAG 1 cut(s) 652
BspPI GGATC 2 cut(s) 45, 698
BsrGI TGTACA 1 cut(s) 186
BsrI ACTGG 1 cut(s) 684
BssECI CCNNGG 2 cut(s) 148, 228
BssMI GATC 4 cut(s) 37, 80, 112, 690
BssT1I CCWWGG 1 cut(s) 148
Bst2UI CCWGG 1 cut(s) 531
Bst4CI ACNGT 3 cut(s) 376, 476, 605
Bst6I CTCTTC 1 cut(s) 579
BstAUI TGTACA 1 cut(s) 186
BstC8I GCNNGC 1 cut(s) 449
BstDEI CTNAG 2 cut(s) 77, 490
BstEII GGTNACC 1 cut(s) 724
BstF5I GGATG 1 cut(s) 712
BstHHI GCGC 1 cut(s) 392
BstKTI GATC 4 cut(s) 40, 83, 115, 693
BstMAI GTCTC 1 cut(s) 393
BstMBI GATC 4 cut(s) 37, 80, 112, 690
BstMWI GCNNNNNNNGC 2 cut(s) 632, 656
BstNI CCWGG 1 cut(s) 531
BstPI GGTNACC 1 cut(s) 724
BstSCI CCNGG 2 cut(s) 19, 529
BstSFI CTRYAG 2 cut(s) 159, 648
BstV1I GCAGC 2 cut(s) 438, 662
BstV2I GAAGAC 1 cut(s) 131
BstX2I RGATCY 2 cut(s) 37, 80
BstYI RGATCY 2 cut(s) 37, 80
BtsCI GGATG 1 cut(s) 712
BtsI GCAGTG 1 cut(s) 399
BtsIMutI CAGTG 2 cut(s) 399, 691
Cac8I GCNNGC 1 cut(s) 449
CfoI GCGC 1 cut(s) 392
Csp6I GTAC 1 cut(s) 187
CviAII CATG 4 cut(s) 52, 275, 329, 406
CviQI GTAC 1 cut(s) 187
DdeI CTNAG 2 cut(s) 77, 490
DpnI GATC 4 cut(s) 39, 82, 114, 692
DpnII GATC 4 cut(s) 37, 80, 112, 690
DraI TTTAAA 1 cut(s) 90
Eam1104I CTCTTC 1 cut(s) 579
EarI CTCTTC 1 cut(s) 579
Eco130I CCWWGG 1 cut(s) 148
Eco57I CTGAAG 2 cut(s) 603, 698
Eco91I GGTNACC 1 cut(s) 724
EcoO65I GGTNACC 1 cut(s) 724
EcoRII CCWGG 1 cut(s) 529
EcoT14I CCWWGG 1 cut(s) 148
EcoT22I ATGCAT 1 cut(s) 53
ErhI CCWWGG 1 cut(s) 148
FaeI CATG 4 cut(s) 55, 278, 332, 409
FaiI YATR 8 cut(s) 53, 276, 288, 302, 330, 407, 480, 545
FatI CATG 4 cut(s) 51, 274, 328, 405
Fnu4HI GCNGC 2 cut(s) 452, 651
FokI GGATG 1 cut(s) 719
Fsp4HI GCNGC 2 cut(s) 452, 651
FspBI CTAG 2 cut(s) 197, 221
FspI TGCGCA 1 cut(s) 391
GlaI GCGC 1 cut(s) 391
GluI GCNGC 2 cut(s) 452, 651
GsaI CCCAGC 1 cut(s) 723
GsuI CTGGAG 1 cut(s) 552
HapII CCGG 1 cut(s) 20
HhaI GCGC 1 cut(s) 392
Hin1II CATG 4 cut(s) 55, 278, 332, 409
Hin6I GCGC 1 cut(s) 390
HinP1I GCGC 1 cut(s) 390
HindIII AAGCTT 1 cut(s) 191
HinfI GANTC 4 cut(s) 56, 95, 164, 271
HpaII CCGG 1 cut(s) 20
HphI GGTGA 4 cut(s) 245, 325, 340, 464
Hpy166II GTNNAC 4 cut(s) 187, 316, 472, 565
Hpy188I TCNGA 7 cut(s) 44, 78, 214, 229, 493, 583, 673
Hpy188III TCNNGA 3 cut(s) 116, 420, 464
Hpy8I GTNNAC 4 cut(s) 187, 316, 472, 565
HpyAV CCTTC 5 cut(s) 301, 374, 545, 583, 654
HpyCH4III ACNGT 3 cut(s) 376, 476, 605
HpyCH4IV ACGT 1 cut(s) 468
HpyCH4V TGCA 3 cut(s) 51, 626, 650
HpyF10VI GCNNNNNNNGC 2 cut(s) 632, 656
HpyF3I CTNAG 2 cut(s) 77, 490
HpySE526I ACGT 1 cut(s) 468
Hsp92II CATG 4 cut(s) 55, 278, 332, 409
HspAI GCGC 1 cut(s) 390
Kzo9I GATC 4 cut(s) 37, 80, 112, 690
LmnI GCTCC 1 cut(s) 4
Lsp1109I GCAGC 2 cut(s) 438, 662
LweI GCATC 1 cut(s) 377
MaeI CTAG 2 cut(s) 197, 221
MaeII ACGT 1 cut(s) 468
MaeIII GTNAC 1 cut(s) 724
MalI GATC 4 cut(s) 39, 82, 114, 692
MboI GATC 4 cut(s) 37, 80, 112, 690
MboII GAAGA 5 cut(s) 131, 396, 518, 560, 596
MfeI CAATTG 1 cut(s) 353
MflI RGATCY 2 cut(s) 37, 80
MluCI AATT 3 cut(s) 241, 353, 411
MmeI TCCRAC 1 cut(s) 684
MnlI CCTC 3 cut(s) 223, 304, 502
Mph1103I ATGCAT 1 cut(s) 53
MroXI GAANNNNTTC 4 cut(s) 60, 99, 415, 555
MseI TTAA 2 cut(s) 89, 293
MspA1I CMGCKG 1 cut(s) 719
MspI CCGG 1 cut(s) 20
MspR9I CCNGG 2 cut(s) 21, 531
MunI CAATTG 1 cut(s) 353
Mva1269I GAATGC 1 cut(s) 53
MvaI CCWGG 1 cut(s) 531
MwoI GCNNNNNNNGC 2 cut(s) 632, 656
NciI CCSGG 1 cut(s) 21
NdeII GATC 4 cut(s) 37, 80, 112, 690
NlaIII CATG 4 cut(s) 55, 278, 332, 409
NsbI TGCGCA 1 cut(s) 391
NsiI ATGCAT 1 cut(s) 53
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PctI GAATGC 1 cut(s) 53
PdmI GAANNNNTTC 4 cut(s) 60, 99, 415, 555
PfeI GAWTC 4 cut(s) 56, 95, 164, 271
PfoI TCCNGGA 1 cut(s) 529
PkrI GCNGC 2 cut(s) 453, 652
Psp6I CCWGG 1 cut(s) 529
PspEI GGTNACC 1 cut(s) 724
PspFI CCCAGC 1 cut(s) 719
PspGI CCWGG 1 cut(s) 529
PstI CTGCAG 1 cut(s) 652
PsuI RGATCY 2 cut(s) 37, 80
PvuII CAGCTG 1 cut(s) 719
RsaI GTAC 1 cut(s) 188
RsaNI GTAC 1 cut(s) 187
SaqAI TTAA 2 cut(s) 89, 293
SatI GCNGC 2 cut(s) 452, 651
Sau3AI GATC 4 cut(s) 37, 80, 112, 690
ScrFI CCNGG 2 cut(s) 21, 531
SfaNI GCATC 1 cut(s) 377
SfcI CTRYAG 2 cut(s) 159, 648
Sse9I AATT 3 cut(s) 241, 353, 411
SsiI CCGC 1 cut(s) 182
SspMI CTAG 2 cut(s) 197, 221
StyD4I CCNGG 2 cut(s) 19, 529
StyI CCWWGG 1 cut(s) 148
TaaI ACNGT 3 cut(s) 376, 476, 605
TaiI ACGT 1 cut(s) 471
TaqI TCGA 1 cut(s) 111
TasI AATT 3 cut(s) 241, 353, 411
TatI WGTACW 1 cut(s) 186
TfiI GAWTC 4 cut(s) 56, 95, 164, 271
Tru1I TTAA 2 cut(s) 89, 293
Tru9I TTAA 2 cut(s) 89, 293
TscAI CASTG 2 cut(s) 399, 691
TseI GCWGC 2 cut(s) 451, 650
TspDTI ATGAA 6 cut(s) 131, 263, 317, 474, 614, 723
TspGWI ACGGA 1 cut(s) 56
TspRI CASTG 2 cut(s) 399, 691
XapI RAATTY 1 cut(s) 241
XcmI CCANNNNNNNNNTGG 1 cut(s) 359
XmnI GAANNNNTTC 4 cut(s) 60, 99, 415, 555
XspI CTAG 2 cut(s) 197, 221
Zsp2I ATGCAT 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.