Rroxscaffold_7G00174820

ASCH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005025
Physical Location & Seq
Reverse (-)
14523506 .. 14524393
888 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00174820.1

Sequence Viewer

Length: 699 bp
ATGGTTGAACCCAATGTGAAGGAGTGCATGGAAGAGCTGTTGAAGTTCACCCTTGAATCTCACATAAAGGGTACCCTGGATATCGATCTAGCCTTGCCCACTGACTTCTGCCTCCTCAACAACCCGAAAATAGGCACCAATTCTACAGGGGGAGAACCCCCATATCCTTTATACAAGCAACTGGCACTAGCCCTATATGAATCCATCAATTCTAATGCATTTTCAGGGACACGTAGTTGGGATCAGAGTTCACAATTACTAAACACTCTCAAGTCTGTGGACTTTGAACTCCATGTTCAAGAGCCCTTTTTCACACAAATCAAAGACGGCCTAAAGACAATCGAAGGAAGGTGCAACGTTGGTAACTATAGTCGAATCGGATCAGGAGCACTGATCCTCTTCAACAAATGCCTGATTGTCGAGGTTAAGGAAGTCCGATCGTATGGTTCGTTCTCTGGTATGTTGAAGGCAGAGAGCCTAGAGAAAGTTCTCCCTGGTGTGAAAACCATTGAAGAGGGTGTTCAAGTCTACAGAAGATTCTACACTGAAGAAATTGAAAAGTCCAGTGGTGTCATTGCCATATGCATTGAAAAATTGCCTGCCCAACCCTATATGTATTTGGCCAAGTTATTATCAGAATTGGGTTATGATGGTATTCAAAGCCTTCTGGAGCTCAATTCACATGAATTCAGTGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ASCH

Protein Analysis

232

Amino Acids

25.88

Weight (kDa)

5.05

Isoelectric Point (pI)

51.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ASCH PF04266 97 - 199 3.8e-15 ASCH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 71
AccB1I GGYRCC 2 cut(s) 71, 134
AccI GTMKAC 1 cut(s) 528
AclI AACGTT 1 cut(s) 357
AclWI GGATC 3 cut(s) 249, 388, 388
AcoI YGGCCR 1 cut(s) 621
AcsI RAATTY 1 cut(s) 686
AcuI CTGAAG 1 cut(s) 567
AfaI GTAC 1 cut(s) 73
AfiI CCNNNNNNNGG 1 cut(s) 131
AflIII ACRYGT 1 cut(s) 230
AjnI CCWGG 2 cut(s) 75, 493
AluBI AGCT 2 cut(s) 37, 673
AluI AGCT 2 cut(s) 37, 673
Alw21I GWGCWC 2 cut(s) 391, 675
AlwI GGATC 3 cut(s) 249, 388, 388
AoxI GGCC 2 cut(s) 328, 621
ApoI RAATTY 1 cut(s) 686
Asp718I GGTACC 1 cut(s) 71
AsuHPI GGTGA 1 cut(s) 40
BalI TGGCCA 1 cut(s) 623
BanI GGYRCC 2 cut(s) 71, 134
BanII GRGCYC 2 cut(s) 306, 675
Bbv12I GWGCWC 2 cut(s) 391, 675
BccI CCATC 2 cut(s) 212, 644
BceAI ACGGC 1 cut(s) 343
BciT130I CCWGG 2 cut(s) 77, 495
BfaI CTAG 3 cut(s) 89, 188, 479
BfmI CTRYAG 3 cut(s) 144, 367, 529
Bme1390I CCNGG 2 cut(s) 77, 495
BmiI GGNNCC 2 cut(s) 73, 136
BmrFI CCNGG 2 cut(s) 77, 495
BpmI CTGGAG 1 cut(s) 689
BpuEI CTTGAG 1 cut(s) 254
Bsa29I ATCGAT 1 cut(s) 84
BsaAI YACGTR 1 cut(s) 233
BsaBI GATNNNNATC 1 cut(s) 84
BsaJI CCNNGG 2 cut(s) 75, 493
Bsc4I CCNNNNNNNGG 1 cut(s) 131
Bse1I ACTGG 2 cut(s) 186, 564
Bse3DI GCAATG 1 cut(s) 573
Bse8I GATNNNNATC 1 cut(s) 84
BseBI CCWGG 2 cut(s) 77, 495
BseCI ATCGAT 1 cut(s) 84
BseDI CCNNGG 2 cut(s) 75, 493
BseJI GATNNNNATC 1 cut(s) 84
BseLI CCNNNNNNNGG 1 cut(s) 131
BseMI GCAATG 1 cut(s) 573
BseNI ACTGG 2 cut(s) 186, 564
BseRI GAGGAG 1 cut(s) 104
Bsh1285I CGRYCG 1 cut(s) 440
BshFI GGCC 2 cut(s) 330, 623
BshNI GGYRCC 2 cut(s) 71, 134
BshVI ATCGAT 1 cut(s) 84
BsiEI CGRYCG 1 cut(s) 440
BsiHKAI GWGCWC 2 cut(s) 391, 675
BslFI GGGAC 1 cut(s) 241
BslI CCNNNNNNNGG 1 cut(s) 131
BsmFI GGGAC 1 cut(s) 241
BsnI GGCC 2 cut(s) 330, 623
Bsp1286I GDGCHC 3 cut(s) 306, 391, 675
Bsp143I GATC 5 cut(s) 85, 241, 380, 393, 437
BspANI GGCC 2 cut(s) 330, 623
BspDI ATCGAT 1 cut(s) 84
BspLI GGNNCC 2 cut(s) 73, 136
BspPI GGATC 3 cut(s) 249, 388, 388
BspQI GCTCTTC 1 cut(s) 27
BspT107I GGYRCC 2 cut(s) 71, 134
BsrDI GCAATG 1 cut(s) 573
BsrI ACTGG 2 cut(s) 186, 564
BssECI CCNNGG 2 cut(s) 75, 493
BssMI GATC 5 cut(s) 85, 241, 380, 393, 437
Bst2UI CCWGG 2 cut(s) 77, 495
Bst6I CTCTTC 3 cut(s) 27, 404, 507
BstBAI YACGTR 1 cut(s) 233
BstC8I GCNNGC 1 cut(s) 600
BstKTI GATC 5 cut(s) 88, 244, 383, 396, 440
BstMBI GATC 5 cut(s) 85, 241, 380, 393, 437
BstMCI CGRYCG 1 cut(s) 440
BstNI CCWGG 2 cut(s) 77, 495
BstSCI CCNGG 2 cut(s) 75, 493
BstSFI CTRYAG 3 cut(s) 144, 367, 529
Bsu15I ATCGAT 1 cut(s) 84
BsuRI GGCC 2 cut(s) 330, 623
BsuTUI ATCGAT 1 cut(s) 84
BtsIMutI CAGTG 5 cut(s) 99, 389, 543, 571, 697
Cac8I GCNNGC 1 cut(s) 600
ClaI ATCGAT 1 cut(s) 84
Csp6I GTAC 1 cut(s) 72
CviAII CATG 3 cut(s) 28, 293, 683
CviJI RGCY 9 cut(s) 37, 92, 191, 304, 330, 477, 623, 663, 673
CviKI_1 RGCY 9 cut(s) 37, 92, 191, 304, 330, 477, 623, 663, 673
CviQI GTAC 1 cut(s) 72
DpnI GATC 5 cut(s) 87, 243, 382, 395, 439
DpnII GATC 5 cut(s) 85, 241, 380, 393, 437
EaeI YGGCCR 1 cut(s) 621
Eam1104I CTCTTC 3 cut(s) 27, 404, 507
EarI CTCTTC 3 cut(s) 27, 404, 507
Ecl136II GAGCTC 1 cut(s) 673
Eco24I GRGCYC 2 cut(s) 306, 675
Eco32I GATATC 1 cut(s) 82
Eco53kI GAGCTC 1 cut(s) 673
Eco57I CTGAAG 1 cut(s) 567
EcoICRI GAGCTC 1 cut(s) 673
EcoRI GAATTC 1 cut(s) 686
EcoRII CCWGG 2 cut(s) 75, 493
EcoRV GATATC 1 cut(s) 82
EcoT22I ATGCAT 2 cut(s) 220, 587
EcoT38I GRGCYC 2 cut(s) 306, 675
FaeI CATG 3 cut(s) 31, 296, 686
FaqI GGGAC 1 cut(s) 241
FatI CATG 3 cut(s) 27, 292, 682
FauNDI CATATG 1 cut(s) 581
FblI GTMKAC 1 cut(s) 528
FriOI GRGCYC 2 cut(s) 306, 675
FspBI CTAG 3 cut(s) 89, 188, 479
GsuI CTGGAG 1 cut(s) 689
HaeIII GGCC 2 cut(s) 330, 623
Hin1II CATG 3 cut(s) 31, 296, 686
HinfI GANTC 4 cut(s) 56, 200, 375, 537
HphI GGTGA 1 cut(s) 40
Hpy166II GTNNAC 4 cut(s) 48, 251, 280, 529
Hpy188I TCNGA 4 cut(s) 246, 380, 437, 637
Hpy188III TCNNGA 3 cut(s) 299, 384, 668
Hpy8I GTNNAC 4 cut(s) 48, 251, 280, 529
HpyAV CCTTC 5 cut(s) 13, 338, 342, 460, 674
HpyCH4IV ACGT 2 cut(s) 232, 357
HpyCH4V TGCA 4 cut(s) 27, 218, 354, 585
HpySE526I ACGT 2 cut(s) 232, 357
Hsp92II CATG 3 cut(s) 31, 296, 686
KpnI GGTACC 1 cut(s) 75
Kzo9I GATC 5 cut(s) 85, 241, 380, 393, 437
LguI GCTCTTC 1 cut(s) 27
LmnI GCTCC 2 cut(s) 386, 670
MaeI CTAG 3 cut(s) 89, 188, 479
MaeII ACGT 2 cut(s) 232, 357
MaeIII GTNAC 1 cut(s) 362
MalI GATC 5 cut(s) 87, 243, 382, 395, 439
MboI GATC 5 cut(s) 85, 241, 380, 393, 437
MboII GAAGA 5 cut(s) 44, 391, 524, 546, 560
MhlI GDGCHC 3 cut(s) 306, 391, 675
MlsI TGGCCA 1 cut(s) 623
MluCI AATT 8 cut(s) 139, 208, 254, 552, 593, 638, 676, 686
MluNI TGGCCA 1 cut(s) 623
MnlI CCTC 5 cut(s) 122, 125, 407, 415, 508
Mox20I TGGCCA 1 cut(s) 623
Mph1103I ATGCAT 2 cut(s) 220, 587
MscI TGGCCA 1 cut(s) 623
MseI TTAA 1 cut(s) 426
Msp20I TGGCCA 1 cut(s) 623
MspR9I CCNGG 2 cut(s) 77, 495
MvaI CCWGG 2 cut(s) 77, 495
NdeI CATATG 1 cut(s) 581
NdeII GATC 5 cut(s) 85, 241, 380, 393, 437
NlaIII CATG 3 cut(s) 31, 296, 686
NlaIV GGNNCC 2 cut(s) 73, 136
NsiI ATGCAT 2 cut(s) 220, 587
PciSI GCTCTTC 1 cut(s) 27
PcsI WCGNNNNNNNCGW 1 cut(s) 446
PfeI GAWTC 4 cut(s) 56, 200, 375, 537
Ple19I CGATCG 1 cut(s) 440
Ppu21I YACGTR 1 cut(s) 233
Psp124BI GAGCTC 1 cut(s) 675
Psp1406I AACGTT 1 cut(s) 357
Psp6I CCWGG 2 cut(s) 75, 493
PspGI CCWGG 2 cut(s) 75, 493
PspN4I GGNNCC 2 cut(s) 73, 136
PsrI GAACNNNNNNTAC 2 cut(s) 434, 466
PvuI CGATCG 1 cut(s) 440
RsaI GTAC 1 cut(s) 73
RsaNI GTAC 1 cut(s) 72
SacI GAGCTC 1 cut(s) 675
SapI GCTCTTC 1 cut(s) 27
SaqAI TTAA 1 cut(s) 426
Sau3AI GATC 5 cut(s) 85, 241, 380, 393, 437
ScrFI CCNGG 2 cut(s) 77, 495
SduI GDGCHC 3 cut(s) 306, 391, 675
SetI ASST 6 cut(s) 39, 235, 353, 360, 426, 675
SfcI CTRYAG 3 cut(s) 144, 367, 529
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
Sse9I AATT 8 cut(s) 139, 208, 254, 552, 593, 638, 676, 686
SspMI CTAG 3 cut(s) 89, 188, 479
SstI GAGCTC 1 cut(s) 675
StyD4I CCNGG 2 cut(s) 75, 493
TaiI ACGT 2 cut(s) 235, 360
TaqI TCGA 4 cut(s) 84, 342, 373, 420
TasI AATT 8 cut(s) 139, 208, 254, 552, 593, 638, 676, 686
TfiI GAWTC 4 cut(s) 56, 200, 375, 537
Tru1I TTAA 1 cut(s) 426
Tru9I TTAA 1 cut(s) 426
TscAI CASTG 5 cut(s) 106, 396, 550, 571, 697
TspDTI ATGAA 2 cut(s) 213, 699
TspRI CASTG 5 cut(s) 106, 396, 550, 571, 697
XapI RAATTY 1 cut(s) 686
XmiI GTMKAC 1 cut(s) 528
XspI CTAG 3 cut(s) 89, 188, 479
Zsp2I ATGCAT 2 cut(s) 220, 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.