Rh6AG342100

ASCH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0005025
Physical Location & Seq
Forward (+)
54141919 .. 54142617
699 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG342100.1

Sequence Viewer

Length: 699 bp
ATGGTTGAACCCAAAGTGAAAGAGTGCATGGAAGAGCTGTTGAAGTTCACCCTTGAATCTCACATAAAGGGTACCCAGGATATCGATCTAGCCTTGCCCACTGACTTCTGCCTCCTCAACAACCCGAAAATCGGCACCAATTCTACAAAGGGAGAACCCCCATATCCTTTATACAAGCAACTAGCACTAGCCCTATATGAATCCATCAATTCTAATGCCATTTCAGGGACAGATAATTGGGATCAGAGTTCACAATTACTAAACACTCTCAAGTCTGTGGACTTTGAACTCCATGTTCAGGAGCCCTTTTTCACACAAATCAAAGACGGCCTAAAGACAGTCGAAGGAAGGTGCAACGTTGGTCCCTATAGTCGAATCGGATCTGGAGCACTGATCCTCTTCAACAAATGCCTGATTGTCGAGGTTAAGGAAGTCCGATCGTATGGTTCCTTCTCCGATATGTTGAAAGCAGAGAGCCTAGAGAAAGTTCTCCCTGGTGTGAAAACCATTGAAGAGGGTGTTCAAGTTTACAGAAGATTCTACACTGAAGAAATTGAAAGGTCCAATGGTGTCATTGCCATATGCATTGAAAAATTGCCTGCCCAACCCTATATGTATTTGGCCAAGTTATTATCAGAATTGGGTAATGATGGTATTCAAAGCCTTTTGGAGCTCAATTCACATGAATTCAGTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ASCH

Protein Analysis

232

Amino Acids

25.97

Weight (kDa)

4.98

Isoelectric Point (pI)

46.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ASCH PF04266 97 - 198 1.1e-15 ASCH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 71
AccB1I GGYRCC 2 cut(s) 71, 134
AclI AACGTT 1 cut(s) 357
AclWI GGATC 3 cut(s) 249, 388, 388
AcoI YGGCCR 1 cut(s) 621
AcsI RAATTY 1 cut(s) 686
AcuI CTGAAG 1 cut(s) 567
AfaI GTAC 1 cut(s) 73
AfiI CCNNNNNNNGG 3 cut(s) 131, 225, 298
AjnI CCWGG 2 cut(s) 75, 493
AluBI AGCT 2 cut(s) 37, 673
AluI AGCT 2 cut(s) 37, 673
Alw21I GWGCWC 2 cut(s) 391, 675
AlwI GGATC 3 cut(s) 249, 388, 388
AoxI GGCC 2 cut(s) 328, 621
ApoI RAATTY 1 cut(s) 686
Asp718I GGTACC 1 cut(s) 71
AspS9I GGNCC 2 cut(s) 362, 561
AsuHPI GGTGA 1 cut(s) 40
AvaII GGWCC 2 cut(s) 362, 561
BalI TGGCCA 1 cut(s) 623
BanI GGYRCC 2 cut(s) 71, 134
BanII GRGCYC 2 cut(s) 306, 675
BarI GAAGNNNNNNTAC 2 cut(s) 434, 466
Bbv12I GWGCWC 2 cut(s) 391, 675
BccI CCATC 2 cut(s) 212, 644
BceAI ACGGC 1 cut(s) 343
BciT130I CCWGG 2 cut(s) 77, 495
BfaI CTAG 4 cut(s) 89, 182, 188, 479
BfmI CTRYAG 1 cut(s) 367
Bme1390I CCNGG 2 cut(s) 77, 495
Bme18I GGWCC 2 cut(s) 362, 561
BmgT120I GGNCC 2 cut(s) 362, 561
BmiI GGNNCC 5 cut(s) 73, 136, 303, 364, 448
BmrFI CCNGG 2 cut(s) 77, 495
BpmI CTGGAG 1 cut(s) 405
BpuEI CTTGAG 1 cut(s) 254
Bsa29I ATCGAT 1 cut(s) 84
BsaBI GATNNNNATC 1 cut(s) 84
BsaJI CCNNGG 2 cut(s) 75, 493
Bsc4I CCNNNNNNNGG 3 cut(s) 131, 225, 298
Bse3DI GCAATG 1 cut(s) 573
Bse8I GATNNNNATC 1 cut(s) 84
BseBI CCWGG 2 cut(s) 77, 495
BseCI ATCGAT 1 cut(s) 84
BseDI CCNNGG 2 cut(s) 75, 493
BseJI GATNNNNATC 1 cut(s) 84
BseLI CCNNNNNNNGG 3 cut(s) 131, 225, 298
BseMI GCAATG 1 cut(s) 573
BseRI GAGGAG 1 cut(s) 104
Bsh1285I CGRYCG 1 cut(s) 440
BshFI GGCC 2 cut(s) 330, 623
BshNI GGYRCC 2 cut(s) 71, 134
BshVI ATCGAT 1 cut(s) 84
BsiEI CGRYCG 1 cut(s) 440
BsiHKAI GWGCWC 2 cut(s) 391, 675
BslFI GGGAC 2 cut(s) 241, 348
BslI CCNNNNNNNGG 3 cut(s) 131, 225, 298
BsmFI GGGAC 2 cut(s) 241, 348
BsnI GGCC 2 cut(s) 330, 623
Bsp1286I GDGCHC 3 cut(s) 306, 391, 675
Bsp143I GATC 5 cut(s) 85, 241, 380, 393, 437
BspANI GGCC 2 cut(s) 330, 623
BspDI ATCGAT 1 cut(s) 84
BspLI GGNNCC 5 cut(s) 73, 136, 303, 364, 448
BspPI GGATC 3 cut(s) 249, 388, 388
BspQI GCTCTTC 1 cut(s) 27
BspT107I GGYRCC 2 cut(s) 71, 134
BsrDI GCAATG 1 cut(s) 573
BssECI CCNNGG 2 cut(s) 75, 493
BssMI GATC 5 cut(s) 85, 241, 380, 393, 437
Bst2UI CCWGG 2 cut(s) 77, 495
Bst4CI ACNGT 1 cut(s) 340
Bst6I CTCTTC 3 cut(s) 27, 404, 507
BstC8I GCNNGC 1 cut(s) 600
BstKTI GATC 5 cut(s) 88, 244, 383, 396, 440
BstMBI GATC 5 cut(s) 85, 241, 380, 393, 437
BstMCI CGRYCG 1 cut(s) 440
BstNI CCWGG 2 cut(s) 77, 495
BstSCI CCNGG 2 cut(s) 75, 493
BstSFI CTRYAG 1 cut(s) 367
BstX2I RGATCY 1 cut(s) 380
BstYI RGATCY 1 cut(s) 380
Bsu15I ATCGAT 1 cut(s) 84
BsuRI GGCC 2 cut(s) 330, 623
BsuTUI ATCGAT 1 cut(s) 84
BtsIMutI CAGTG 4 cut(s) 99, 389, 543, 697
Cac8I GCNNGC 1 cut(s) 600
Cfr13I GGNCC 2 cut(s) 362, 561
ClaI ATCGAT 1 cut(s) 84
Csp6I GTAC 1 cut(s) 72
CviAII CATG 3 cut(s) 28, 293, 683
CviJI RGCY 9 cut(s) 37, 92, 191, 304, 330, 477, 623, 663, 673
CviKI_1 RGCY 9 cut(s) 37, 92, 191, 304, 330, 477, 623, 663, 673
CviQI GTAC 1 cut(s) 72
DpnI GATC 5 cut(s) 87, 243, 382, 395, 439
DpnII GATC 5 cut(s) 85, 241, 380, 393, 437
EaeI YGGCCR 1 cut(s) 621
Eam1104I CTCTTC 3 cut(s) 27, 404, 507
EarI CTCTTC 3 cut(s) 27, 404, 507
Ecl136II GAGCTC 1 cut(s) 673
Eco24I GRGCYC 2 cut(s) 306, 675
Eco32I GATATC 1 cut(s) 82
Eco47I GGWCC 2 cut(s) 362, 561
Eco53kI GAGCTC 1 cut(s) 673
Eco57I CTGAAG 1 cut(s) 567
EcoICRI GAGCTC 1 cut(s) 673
EcoRI GAATTC 1 cut(s) 686
EcoRII CCWGG 2 cut(s) 75, 493
EcoRV GATATC 1 cut(s) 82
EcoT22I ATGCAT 1 cut(s) 587
EcoT38I GRGCYC 2 cut(s) 306, 675
FaeI CATG 3 cut(s) 31, 296, 686
FaqI GGGAC 2 cut(s) 241, 348
FatI CATG 3 cut(s) 27, 292, 682
FauNDI CATATG 1 cut(s) 581
FriOI GRGCYC 2 cut(s) 306, 675
FspBI CTAG 4 cut(s) 89, 182, 188, 479
GsuI CTGGAG 1 cut(s) 405
HaeIII GGCC 2 cut(s) 330, 623
Hin1II CATG 3 cut(s) 31, 296, 686
HinfI GANTC 4 cut(s) 56, 200, 375, 537
HphI GGTGA 1 cut(s) 40
Hpy166II GTNNAC 4 cut(s) 48, 251, 280, 529
Hpy188I TCNGA 5 cut(s) 246, 380, 437, 457, 637
Hpy188III TCNNGA 2 cut(s) 299, 384
Hpy8I GTNNAC 4 cut(s) 48, 251, 280, 529
HpyAV CCTTC 3 cut(s) 338, 342, 460
HpyCH4III ACNGT 1 cut(s) 340
HpyCH4IV ACGT 1 cut(s) 357
HpyCH4V TGCA 4 cut(s) 27, 354, 585, 695
HpySE526I ACGT 1 cut(s) 357
Hsp92II CATG 3 cut(s) 31, 296, 686
KpnI GGTACC 1 cut(s) 75
Kzo9I GATC 5 cut(s) 85, 241, 380, 393, 437
LguI GCTCTTC 1 cut(s) 27
LmnI GCTCC 3 cut(s) 301, 386, 670
LpnPI CCDG 9 cut(s) 62, 89, 210, 284, 369, 425, 480, 507, 612
MaeI CTAG 4 cut(s) 89, 182, 188, 479
MaeII ACGT 1 cut(s) 357
MalI GATC 5 cut(s) 87, 243, 382, 395, 439
MboI GATC 5 cut(s) 85, 241, 380, 393, 437
MboII GAAGA 5 cut(s) 44, 391, 524, 546, 560
MflI RGATCY 1 cut(s) 380
MhlI GDGCHC 3 cut(s) 306, 391, 675
MlsI TGGCCA 1 cut(s) 623
MluCI AATT 9 cut(s) 139, 208, 235, 254, 552, 593, 638, 676, 686
MluNI TGGCCA 1 cut(s) 623
MnlI CCTC 5 cut(s) 122, 125, 407, 415, 508
Mox20I TGGCCA 1 cut(s) 623
Mph1103I ATGCAT 1 cut(s) 587
MscI TGGCCA 1 cut(s) 623
MseI TTAA 1 cut(s) 426
Msp20I TGGCCA 1 cut(s) 623
MspR9I CCNGG 2 cut(s) 77, 495
MvaI CCWGG 2 cut(s) 77, 495
NdeI CATATG 1 cut(s) 581
NdeII GATC 5 cut(s) 85, 241, 380, 393, 437
NlaIII CATG 3 cut(s) 31, 296, 686
NlaIV GGNNCC 5 cut(s) 73, 136, 303, 364, 448
NsiI ATGCAT 1 cut(s) 587
PciSI GCTCTTC 1 cut(s) 27
PfeI GAWTC 4 cut(s) 56, 200, 375, 537
Ple19I CGATCG 1 cut(s) 440
Psp124BI GAGCTC 1 cut(s) 675
Psp1406I AACGTT 1 cut(s) 357
Psp6I CCWGG 2 cut(s) 75, 493
PspGI CCWGG 2 cut(s) 75, 493
PspN4I GGNNCC 5 cut(s) 73, 136, 303, 364, 448
PspPI GGNCC 2 cut(s) 362, 561
PsuI RGATCY 1 cut(s) 380
PvuI CGATCG 1 cut(s) 440
RsaI GTAC 1 cut(s) 73
RsaNI GTAC 1 cut(s) 72
SacI GAGCTC 1 cut(s) 675
SapI GCTCTTC 1 cut(s) 27
SaqAI TTAA 1 cut(s) 426
Sau3AI GATC 5 cut(s) 85, 241, 380, 393, 437
Sau96I GGNCC 2 cut(s) 362, 561
ScrFI CCNGG 2 cut(s) 77, 495
SduI GDGCHC 3 cut(s) 306, 391, 675
SetI ASST 6 cut(s) 39, 353, 360, 426, 563, 675
SfcI CTRYAG 1 cut(s) 367
SinI GGWCC 2 cut(s) 362, 561
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
Sse9I AATT 9 cut(s) 139, 208, 235, 254, 552, 593, 638, 676, 686
SspMI CTAG 4 cut(s) 89, 182, 188, 479
SstI GAGCTC 1 cut(s) 675
StyD4I CCNGG 2 cut(s) 75, 493
TaaI ACNGT 1 cut(s) 340
TaiI ACGT 1 cut(s) 360
TaqI TCGA 4 cut(s) 84, 342, 373, 420
TasI AATT 9 cut(s) 139, 208, 235, 254, 552, 593, 638, 676, 686
TfiI GAWTC 4 cut(s) 56, 200, 375, 537
Tru1I TTAA 1 cut(s) 426
Tru9I TTAA 1 cut(s) 426
TscAI CASTG 4 cut(s) 106, 396, 550, 697
TspDTI ATGAA 2 cut(s) 213, 699
TspRI CASTG 4 cut(s) 106, 396, 550, 697
VpaK11BI GGWCC 2 cut(s) 362, 561
XapI RAATTY 1 cut(s) 686
XspI CTAG 4 cut(s) 89, 182, 188, 479
Zsp2I ATGCAT 1 cut(s) 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.