FvH4_2g24830

ASCH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005025
Physical Location & Seq
Forward (+)
20201742 .. 20202843
1102 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g24830.t1

Sequence Viewer

Length: 720 bp
ATGGCTAAACCCAAAGTGAAAGAGTGCTTGGAAGAGCTCTTGAAGTTCACCCTTGAATCTCACATAAAGGGTACCCTGGAAATCGACCTAGCCTTGCCAACTGACTTCTGCTCTCATCTCCTCAACAACCCGAAAATCGACACCACCAATTCCACACAGGAAGAAGGAAAGGAAAAAACCCCAAATCCTTTATACAAGCAACTAGCACTAGCCCTATTCGAATCCATCACTTCCAATGCATGTTCTGAGACACATAGTTTCGATCAGGGTTCTAAAATCCTAAACACTCTCAAGTCTGTGGACTTTGAACTCCATGTTCAGGAGCCCTTTTTCACACAAATCAAAGACGGCCTAAAGACAATAGAAGGAAGGTGCAATGTTGGTGACTACAGTCGAATCGGATCAGGAGCACTGATCCTCTTCAACAAATGCCTGATTGTCGAGGTTAAGGAGGTCCGATCGTATGGTTCGTTCTCGGATATGTTGAAGGCAGAGAGCCTGGAGAAGGTTCTTCCTGGTGTGAAAACCATTGAAGAAGGTGTTCAAGCCTACAGGAGATTCTACACTGAAGAAACTGGAAGGTCCAATGGTGTCATTGCCATATGCATCGAGAAATTGCCTGCCCAAGTCTACATGTATTTTGCTAAATTATTGTCCGATTTGGGTTGTGAGGGTATTCAAAGCCTCTTGCAGCTCAATTCACATGAATTCAGTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ASCH

Protein Analysis

240

Amino Acids

26.71

Weight (kDa)

5.37

Isoelectric Point (pI)

44.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ASCH PF04266 104 - 205 8.2e-15 ASCH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 71
AccB1I GGYRCC 1 cut(s) 71
AccI GTMKAC 1 cut(s) 630
AclWI GGATC 2 cut(s) 409, 409
AcsI RAATTY 1 cut(s) 707
AcuI CTGAAG 1 cut(s) 588
AfaI GTAC 1 cut(s) 73
AfiI CCNNNNNNNGG 2 cut(s) 319, 505
AflIII ACRYGT 1 cut(s) 633
AgsI TTSAA 8 cut(s) 43, 56, 308, 424, 487, 533, 545, 680
AjnI CCWGG 3 cut(s) 75, 498, 514
AjuI GAANNNNNNNTTGG 2 cut(s) 11, 43
AluBI AGCT 2 cut(s) 37, 694
AluI AGCT 2 cut(s) 37, 694
Alw21I GWGCWC 2 cut(s) 39, 412
Alw26I GTCTC 1 cut(s) 242
AlwI GGATC 2 cut(s) 409, 409
AoxI GGCC 1 cut(s) 349
ApeKI GCWGC 1 cut(s) 691
ApoI RAATTY 1 cut(s) 707
Asp700I GAANNNNTTC 1 cut(s) 540
Asp718I GGTACC 1 cut(s) 71
AspS9I GGNCC 2 cut(s) 454, 582
AsuHPI GGTGA 2 cut(s) 40, 395
AsuII TTCGAA 1 cut(s) 219
AvaII GGWCC 2 cut(s) 454, 582
BanI GGYRCC 1 cut(s) 71
BanII GRGCYC 2 cut(s) 39, 327
Bbv12I GWGCWC 2 cut(s) 39, 412
BbvI GCAGC 1 cut(s) 703
BccI CCATC 1 cut(s) 233
BceAI ACGGC 1 cut(s) 364
BciT130I CCWGG 3 cut(s) 77, 500, 516
BcoDI GTCTC 1 cut(s) 242
BfaI CTAG 3 cut(s) 89, 203, 209
BfmI CTRYAG 2 cut(s) 388, 550
BisI GCNGC 1 cut(s) 692
BlsI GCNGC 1 cut(s) 693
Bme1390I CCNGG 3 cut(s) 77, 500, 516
Bme18I GGWCC 2 cut(s) 454, 582
BmgT120I GGNCC 2 cut(s) 454, 582
BmiI GGNNCC 2 cut(s) 73, 324
BmrFI CCNGG 3 cut(s) 77, 500, 516
BmsI GCATC 1 cut(s) 615
BoxI GACNNNNGTC 1 cut(s) 390
BpmI CTGGAG 1 cut(s) 521
Bpu14I TTCGAA 1 cut(s) 219
BpuEI CTTGAG 1 cut(s) 275
BsaJI CCNNGG 1 cut(s) 75
Bsc4I CCNNNNNNNGG 2 cut(s) 319, 505
Bse1I ACTGG 1 cut(s) 580
Bse3DI GCAATG 2 cut(s) 382, 594
BseBI CCWGG 3 cut(s) 77, 500, 516
BseDI CCNNGG 1 cut(s) 75
BseLI CCNNNNNNNGG 2 cut(s) 319, 505
BseMI GCAATG 2 cut(s) 382, 594
BseMII CTCAG 1 cut(s) 237
BseNI ACTGG 1 cut(s) 580
BseRI GAGGAG 1 cut(s) 110
BseXI GCAGC 1 cut(s) 703
Bsh1285I CGRYCG 1 cut(s) 461
BshFI GGCC 1 cut(s) 351
BshNI GGYRCC 1 cut(s) 71
BsiEI CGRYCG 1 cut(s) 461
BsiHKAI GWGCWC 2 cut(s) 39, 412
BslI CCNNNNNNNGG 2 cut(s) 319, 505
BsmAI GTCTC 1 cut(s) 242
BsnI GGCC 1 cut(s) 351
Bsp119I TTCGAA 1 cut(s) 219
Bsp1286I GDGCHC 3 cut(s) 39, 327, 412
Bsp143I GATC 4 cut(s) 262, 401, 414, 458
BspANI GGCC 1 cut(s) 351
BspCNI CTCAG 1 cut(s) 238
BspLI GGNNCC 2 cut(s) 73, 324
BspPI GGATC 2 cut(s) 409, 409
BspQI GCTCTTC 1 cut(s) 27
BspT104I TTCGAA 1 cut(s) 219
BspT107I GGYRCC 1 cut(s) 71
BsrDI GCAATG 2 cut(s) 382, 594
BsrI ACTGG 1 cut(s) 580
BssECI CCNNGG 1 cut(s) 75
BssMI GATC 4 cut(s) 262, 401, 414, 458
Bst2UI CCWGG 3 cut(s) 77, 500, 516
Bst4CI ACNGT 1 cut(s) 392
Bst6I CTCTTC 2 cut(s) 27, 425
BstBI TTCGAA 1 cut(s) 219
BstC8I GCNNGC 1 cut(s) 621
BstDEI CTNAG 1 cut(s) 246
BstENI CCTNNNNNAGG 1 cut(s) 503
BstKTI GATC 4 cut(s) 265, 404, 417, 461
BstMAI GTCTC 1 cut(s) 242
BstMBI GATC 4 cut(s) 262, 401, 414, 458
BstMCI CGRYCG 1 cut(s) 461
BstNI CCWGG 3 cut(s) 77, 500, 516
BstNSI RCATGY 2 cut(s) 243, 637
BstPAI GACNNNNGTC 1 cut(s) 390
BstSCI CCNGG 3 cut(s) 75, 498, 514
BstSFI CTRYAG 2 cut(s) 388, 550
BstV1I GCAGC 1 cut(s) 703
BsuRI GGCC 1 cut(s) 351
BtsIMutI CAGTG 3 cut(s) 410, 564, 718
Cac8I GCNNGC 1 cut(s) 621
Cfr13I GGNCC 2 cut(s) 454, 582
Csp6I GTAC 1 cut(s) 72
CviAII CATG 4 cut(s) 240, 314, 634, 704
CviQI GTAC 1 cut(s) 72
DdeI CTNAG 1 cut(s) 246
DpnI GATC 4 cut(s) 264, 403, 416, 460
DpnII GATC 4 cut(s) 262, 401, 414, 458
Eam1104I CTCTTC 2 cut(s) 27, 425
EarI CTCTTC 2 cut(s) 27, 425
Ecl136II GAGCTC 1 cut(s) 37
Eco24I GRGCYC 2 cut(s) 39, 327
Eco47I GGWCC 2 cut(s) 454, 582
Eco53kI GAGCTC 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 588
EcoICRI GAGCTC 1 cut(s) 37
EcoNI CCTNNNNNAGG 1 cut(s) 503
EcoRI GAATTC 1 cut(s) 707
EcoRII CCWGG 3 cut(s) 75, 498, 514
EcoT22I ATGCAT 2 cut(s) 241, 608
EcoT38I GRGCYC 2 cut(s) 39, 327
FaeI CATG 4 cut(s) 243, 317, 637, 707
FatI CATG 4 cut(s) 239, 313, 633, 703
FauNDI CATATG 1 cut(s) 602
FblI GTMKAC 1 cut(s) 630
Fnu4HI GCNGC 1 cut(s) 692
FriOI GRGCYC 2 cut(s) 39, 327
Fsp4HI GCNGC 1 cut(s) 692
FspBI CTAG 3 cut(s) 89, 203, 209
GluI GCNGC 1 cut(s) 692
GsuI CTGGAG 1 cut(s) 521
HaeIII GGCC 1 cut(s) 351
Hin1II CATG 4 cut(s) 243, 317, 637, 707
HinfI GANTC 4 cut(s) 56, 221, 396, 558
HphI GGTGA 2 cut(s) 40, 395
Hpy166II GTNNAC 3 cut(s) 48, 301, 631
Hpy188I TCNGA 5 cut(s) 247, 401, 458, 478, 658
Hpy188III TCNNGA 4 cut(s) 40, 320, 405, 610
Hpy8I GTNNAC 3 cut(s) 48, 301, 631
HpyAV CCTTC 7 cut(s) 158, 359, 363, 481, 499, 530, 573
HpyCH4III ACNGT 1 cut(s) 392
HpyCH4V TGCA 4 cut(s) 239, 375, 606, 691
HpyF3I CTNAG 1 cut(s) 246
Hsp92II CATG 4 cut(s) 243, 317, 637, 707
KpnI GGTACC 1 cut(s) 75
Kzo9I GATC 4 cut(s) 262, 401, 414, 458
LguI GCTCTTC 1 cut(s) 27
LmnI GCTCC 2 cut(s) 322, 407
Lsp1109I GCAGC 1 cut(s) 703
LweI GCATC 1 cut(s) 615
MaeI CTAG 3 cut(s) 89, 203, 209
MaeIII GTNAC 1 cut(s) 383
MalI GATC 4 cut(s) 264, 403, 416, 460
MboI GATC 4 cut(s) 262, 401, 414, 458
MboII GAAGA 6 cut(s) 44, 173, 412, 503, 545, 581
MhlI GDGCHC 3 cut(s) 39, 327, 412
MluCI AATT 5 cut(s) 148, 614, 647, 697, 707
MnlI CCTC 6 cut(s) 131, 428, 436, 445, 664, 695
Mph1103I ATGCAT 2 cut(s) 241, 608
MroXI GAANNNNTTC 1 cut(s) 540
MseI TTAA 1 cut(s) 447
MspR9I CCNGG 3 cut(s) 77, 500, 516
MvaI CCWGG 3 cut(s) 77, 500, 516
NdeI CATATG 1 cut(s) 602
NdeII GATC 4 cut(s) 262, 401, 414, 458
NlaIII CATG 4 cut(s) 243, 317, 637, 707
NlaIV GGNNCC 2 cut(s) 73, 324
NmuCI GTSAC 1 cut(s) 383
NsiI ATGCAT 2 cut(s) 241, 608
NspI RCATGY 2 cut(s) 243, 637
NspV TTCGAA 1 cut(s) 219
PciI ACATGT 1 cut(s) 633
PciSI GCTCTTC 1 cut(s) 27
PcsI WCGNNNNNNNCGW 1 cut(s) 467
PdmI GAANNNNTTC 1 cut(s) 540
PfeI GAWTC 4 cut(s) 56, 221, 396, 558
PkrI GCNGC 1 cut(s) 693
Ple19I CGATCG 1 cut(s) 461
PscI ACATGT 1 cut(s) 633
PshAI GACNNNNGTC 1 cut(s) 390
Psp124BI GAGCTC 1 cut(s) 39
Psp6I CCWGG 3 cut(s) 75, 498, 514
PspGI CCWGG 3 cut(s) 75, 498, 514
PspN4I GGNNCC 2 cut(s) 73, 324
PspPI GGNCC 2 cut(s) 454, 582
PsrI GAACNNNNNNTAC 2 cut(s) 455, 487
PvuI CGATCG 1 cut(s) 461
RsaI GTAC 1 cut(s) 73
RsaNI GTAC 1 cut(s) 72
SacI GAGCTC 1 cut(s) 39
SapI GCTCTTC 1 cut(s) 27
SaqAI TTAA 1 cut(s) 447
SatI GCNGC 1 cut(s) 692
Sau3AI GATC 4 cut(s) 262, 401, 414, 458
Sau96I GGNCC 2 cut(s) 454, 582
ScrFI CCNGG 3 cut(s) 77, 500, 516
SduI GDGCHC 3 cut(s) 39, 327, 412
SetI ASST 9 cut(s) 39, 90, 374, 447, 456, 510, 541, 584, 696
SfaNI GCATC 1 cut(s) 615
SfcI CTRYAG 2 cut(s) 388, 550
SfuI TTCGAA 1 cut(s) 219
SinI GGWCC 2 cut(s) 454, 582
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
Sse9I AATT 5 cut(s) 148, 614, 647, 697, 707
SspMI CTAG 3 cut(s) 89, 203, 209
SstI GAGCTC 1 cut(s) 39
StyD4I CCNGG 3 cut(s) 75, 498, 514
TaaI ACNGT 1 cut(s) 392
TaqI TCGA 7 cut(s) 84, 138, 219, 261, 394, 441, 609
TasI AATT 5 cut(s) 148, 614, 647, 697, 707
TfiI GAWTC 4 cut(s) 56, 221, 396, 558
Tru1I TTAA 1 cut(s) 447
Tru9I TTAA 1 cut(s) 447
TscAI CASTG 3 cut(s) 417, 571, 718
TseFI GTSAC 1 cut(s) 383
TseI GCWGC 1 cut(s) 691
Tsp45I GTSAC 1 cut(s) 383
TspDTI ATGAA 1 cut(s) 720
TspRI CASTG 3 cut(s) 417, 571, 718
VpaK11BI GGWCC 2 cut(s) 454, 582
XagI CCTNNNNNAGG 1 cut(s) 503
XapI RAATTY 1 cut(s) 707
XceI RCATGY 2 cut(s) 243, 637
XmiI GTMKAC 1 cut(s) 630
XmnI GAANNNNTTC 1 cut(s) 540
XspI CTAG 3 cut(s) 89, 203, 209
Zsp2I ATGCAT 2 cut(s) 241, 608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.