AT2G44260

Vacuolar protein sorting-associated protein 62

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0009493
Physical Location & Seq
Forward (+)
18295838 .. 18297867
2030 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G44260.2

Sequence Viewer

Length: 1662 bp
ATGGGAAACTGCCTTTCAACCTCTGATCCATCTCATGAAGATGTATCAAAGAAGCTTCCCAAAGCACTTCCCGTCGATGCTGCATTCAAGTTTCCTTCACCGTTACCTACTTTTACTCGAGGTGATGGTTTTGCGAAAGGAACCATTGACTTAGGAGGTGGTCTCGAAGTTAGTCAAGTTTCGACATTCAACAAAGTTTGGTCCACTTATGAAGGAGGACCAGACAATCTCGGAGCTACATTCTTTGAACCATCCTCAATCCCTTCCGGTTTCTCCATCCTTGGCTACTACGCTCAACCAAACAACCGTAATCTTTTCGGTTGGGTACTCACAGCTAGAGATCTCTCAAGCAACACCTTAAAACCACCAGTAGATTACACCCTCGTAGGAAACACCGAGTCACTCAAAATTAAACAAGACGGAACCGGATACTTCTGGCAACCCGTTCCTCCTGATGGATATCAAGCCGTTGGCCTAATAGTCACAAACTACTCACAAAAACCACCTCTAGACAAACTACGCTGCATCCGATCAGATTTAACCGAACAATGTGAAGCTGATACATGGATATGGGGCACAAACGGAGTCAACATCTCAAATCTAAAACCAACCACAAGAGGAACACAAGCTACAGGAGTCTATGTAGGTACATTTACATGGCAAACCCAAAACTCCTCGCCTCCATCATTATCTTGCTTAAAGAACACGAAACTCGACTTCTCTACAATGCCAAATGGGTCCCAAATCGAAGAATTGTTCCAAACTTTTTCTCCATGTATCTATTTCCATCCAGACGAAGAATATCTACCTTCCTCTGTCACTTGGTACTTCAACAACGGAGCATTACTATACAAGAAAGGCGAAGAATCGAAACCAATCCCTATCGAATCAAACGGTTCTAATCTTCCACAAGGAGGATCAAACGATGGATCATACTGGCTAGATCTTCCTATAGACAAAAATGGTAAAGAGAGAGTCAAAAAAGGAGACTTACAAAGCACAAAAGTGTACCTTCATATCAAACCAATGCTCGGAGCAACATTCACAGATATATCAATCTGGATATTTTACCCTTTCAATGGACCAGCAAAAGCCAAGGTCAAATTCGTAAATCTACCGTTGGGTAGAATCGGAGAACACATTGGAGATTGGGAACACACGACGCTACGGATCAGTAATTTCACCGGAGAGCTTTGGAGAGTGTTCTTATCGCAGCATAGTGGTGGAATTTGGATCGATGCTTGCGATTTGGAGTTCCAAGACGGAGGAAATAACAAGTTTGTGGCGTACGCATCGCTACATGGACACGCGATGTATCCGAAACCTGGGTTGGTGTTGCAAGGAGACGATGGGGTTGGGATTAGGAATGATACGGGGAAAGGTAAGAAGGTGCTTGATACGGGGTTAGGGTATGAGGTGATTGCGGCGGAGTATGACGGCGGAGGAGTGGTGGAGCCGCCGTGGGTTAAGTATTTCAGGAAGTGGGGGCCGAAGATTGATTATAATGTTGATGATGAAGTTAAGAGTGTGGAGAGGATATTGCCTGGGCTTTTGAAAAAGGCTTTTGTTAAATTTGTGAAGAAGATTCCTGATGAAGTTTATGGTGAAGATGGGCCTACTGGGCCTAAACTCAAGAGTAATTGGGCTGGTGATGAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps62

Protein Analysis

553

Amino Acids

60.95

Weight (kDa)

5.74

Isoelectric Point (pI)

30.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps62 PF06101 66 - 582 3.9e-248 Vacuolar protein sorting-associated protein 62
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1503
AccII CGCG 1 cut(s) 1310
AciI CCGC 4 cut(s) 1424, 1427, 1440, 1457
AclWI GGATC 5 cut(s) 20, 925, 937, 1178, 1241
AcsI RAATTY 3 cut(s) 1103, 1227, 1571
AfaI GTAC 5 cut(s) 327, 649, 827, 1010, 1289
AfiI CCNNNNNNNGG 5 cut(s) 455, 914, 1031, 1079, 1325
AgsI TTSAA 7 cut(s) 18, 88, 190, 248, 832, 1078, 1555
AjnI CCWGG 2 cut(s) 1324, 1543
AjuI GAANNNNNNNTTGG 4 cut(s) 1088, 1120, 1313, 1345
AleI CACNNNNGTG 1 cut(s) 1004
AluBI AGCT 6 cut(s) 55, 236, 335, 557, 629, 1192
AluI AGCT 6 cut(s) 55, 236, 335, 557, 629, 1192
Alw26I GTCTC 3 cut(s) 167, 981, 1338
AlwI GGATC 5 cut(s) 20, 925, 937, 1178, 1241
Ama87I CYCGRG 1 cut(s) 117
AoxI GGCC 4 cut(s) 472, 1487, 1613, 1622
ApeKI GCWGC 3 cut(s) 80, 522, 1213
ApoI RAATTY 3 cut(s) 1103, 1227, 1571
AspS9I GGNCC 7 cut(s) 201, 218, 738, 1082, 1487, 1613, 1622
AsuHPI GGTGA 6 cut(s) 90, 134, 1174, 1429, 1616, 1661
AvaI CYCGRG 1 cut(s) 117
AvaII GGWCC 4 cut(s) 201, 218, 738, 1082
BaeGI GKGCMC 1 cut(s) 578
BarI GAAGNNNNNNTAC 2 cut(s) 789, 821
BbvI GCAGC 3 cut(s) 67, 509, 1225
BceAI ACGGC 3 cut(s) 452, 1444, 1453
BciT130I CCWGG 2 cut(s) 1326, 1545
BciVI GTATCC 2 cut(s) 422, 1326
BcoDI GTCTC 3 cut(s) 167, 981, 1338
BfaI CTAG 3 cut(s) 336, 509, 941
BfmI CTRYAG 2 cut(s) 630, 951
BfuI GTATCC 2 cut(s) 422, 1326
BglI GCCNNNNNGGC 1 cut(s) 1621
BglII AGATCT 2 cut(s) 340, 943
BisI GCNGC 5 cut(s) 81, 523, 1214, 1425, 1457
BlsI GCNGC 5 cut(s) 82, 524, 1215, 1426, 1458
Bme1390I CCNGG 2 cut(s) 1326, 1545
Bme18I GGWCC 4 cut(s) 201, 218, 738, 1082
BmeT110I CYCGRG 1 cut(s) 117
BmgT120I GGNCC 7 cut(s) 201, 218, 738, 1082, 1487, 1613, 1622
BmiI GGNNCC 6 cut(s) 142, 424, 739, 740, 1455, 1488
BmrFI CCNGG 2 cut(s) 1326, 1545
BmrI ACTGGG 1 cut(s) 1629
BmsI GCATC 4 cut(s) 67, 534, 1228, 1301
BmuI ACTGGG 1 cut(s) 1629
BplI GAGNNNNNCTC 2 cut(s) 147, 179
BpuEI CTTGAG 2 cut(s) 331, 1616
Bsa29I ATCGAT 1 cut(s) 1236
BsaBI GATNNNNATC 1 cut(s) 459
BsaI GGTCTC 1 cut(s) 167
BsaJI CCNNGG 5 cut(s) 280, 1095, 1325, 1460, 1544
BsaWI WCCGGW 3 cut(s) 266, 425, 1184
BsaXI ACNNNNNCTCC 8 cut(s) 627, 657, 754, 784, 1434, 1445, 1464, 1475
Bsc4I CCNNNNNNNGG 5 cut(s) 455, 914, 1031, 1079, 1325
Bse1I ACTGG 3 cut(s) 368, 941, 1624
Bse8I GATNNNNATC 1 cut(s) 459
BseBI CCWGG 2 cut(s) 1326, 1545
BseCI ATCGAT 1 cut(s) 1236
BseDI CCNNGG 5 cut(s) 280, 1095, 1325, 1460, 1544
BseGI GGATG 4 cut(s) 251, 276, 525, 787
BseJI GATNNNNATC 1 cut(s) 459
BseLI CCNNNNNNNGG 5 cut(s) 455, 914, 1031, 1079, 1325
BseNI ACTGG 3 cut(s) 368, 941, 1624
BseRI GAGGAG 2 cut(s) 664, 1458
BseSI GKGCMC 1 cut(s) 578
BseXI GCAGC 3 cut(s) 67, 509, 1225
Bsh1236I CGCG 1 cut(s) 1310
BshFI GGCC 4 cut(s) 474, 1489, 1615, 1624
BshVI ATCGAT 1 cut(s) 1236
BsiHKCI CYCGRG 1 cut(s) 117
BsiSI CCGG 3 cut(s) 267, 426, 1185
BsiWI CGTACG 1 cut(s) 1287
BslFI GGGAC 1 cut(s) 724
BslI CCNNNNNNNGG 5 cut(s) 455, 914, 1031, 1079, 1325
BsmAI GTCTC 3 cut(s) 167, 981, 1338
BsmBI CGTCTC 1 cut(s) 1338
BsmFI GGGAC 1 cut(s) 724
BsmI GAATGC 1 cut(s) 83
BsnI GGCC 4 cut(s) 474, 1489, 1615, 1624
Bso31I GGTCTC 1 cut(s) 167
BsoBI CYCGRG 1 cut(s) 117
Bsp1286I GDGCHC 1 cut(s) 578
Bsp143I GATC 8 cut(s) 25, 340, 530, 917, 929, 943, 1170, 1233
BspACI CCGC 4 cut(s) 1424, 1427, 1440, 1457
BspANI GGCC 4 cut(s) 474, 1489, 1615, 1624
BspDI ATCGAT 1 cut(s) 1236
BspFNI CGCG 1 cut(s) 1310
BspHI TCATGA 1 cut(s) 34
BspLI GGNNCC 6 cut(s) 142, 424, 739, 740, 1455, 1488
BspPI GGATC 5 cut(s) 20, 925, 937, 1178, 1241
BspTNI GGTCTC 1 cut(s) 167
BsrI ACTGG 3 cut(s) 368, 941, 1624
BssECI CCNNGG 5 cut(s) 280, 1095, 1325, 1460, 1544
BssMI GATC 8 cut(s) 25, 340, 530, 917, 929, 943, 1170, 1233
BssT1I CCWWGG 2 cut(s) 280, 1095
Bst2UI CCWGG 2 cut(s) 1326, 1545
Bst4CI ACNGT 4 cut(s) 102, 308, 896, 1119
BstC8I GCNNGC 1 cut(s) 1243
BstDEI CTNAG 1 cut(s) 151
BstDSI CCRYGG 1 cut(s) 1460
BstF5I GGATG 4 cut(s) 251, 276, 525, 787
BstFNI CGCG 1 cut(s) 1310
BstKTI GATC 8 cut(s) 28, 343, 533, 920, 932, 946, 1173, 1236
BstMAI GTCTC 3 cut(s) 167, 981, 1338
BstMBI GATC 8 cut(s) 25, 340, 530, 917, 929, 943, 1170, 1233
BstMWI GCNNNNNNNGC 1 cut(s) 1621
BstNI CCWGG 2 cut(s) 1326, 1545
BstSCI CCNGG 2 cut(s) 1324, 1543
BstSFI CTRYAG 2 cut(s) 630, 951
BstSLI GKGCMC 1 cut(s) 578
BstUI CGCG 1 cut(s) 1310
BstV1I GCAGC 3 cut(s) 67, 509, 1225
BstX2I RGATCY 2 cut(s) 340, 943
BstYI RGATCY 2 cut(s) 340, 943
Bsu15I ATCGAT 1 cut(s) 1236
BsuI GTATCC 2 cut(s) 422, 1326
BsuRI GGCC 4 cut(s) 474, 1489, 1615, 1624
BsuTUI ATCGAT 1 cut(s) 1236
BtgI CCRYGG 1 cut(s) 1460
BtgZI GCGATG 2 cut(s) 1278, 1325
BtsCI GGATG 4 cut(s) 251, 276, 525, 787
Cac8I GCNNGC 1 cut(s) 1243
CciI TCATGA 1 cut(s) 34
Cfr13I GGNCC 7 cut(s) 201, 218, 738, 1082, 1487, 1613, 1622
ClaI ATCGAT 1 cut(s) 1236
CseI GACGC 1 cut(s) 1171
Csp6I GTAC 5 cut(s) 326, 648, 826, 1009, 1288
CviAII CATG 5 cut(s) 35, 564, 657, 774, 1301
CviQI GTAC 5 cut(s) 326, 648, 826, 1009, 1288
DdeI CTNAG 1 cut(s) 151
DpnI GATC 8 cut(s) 27, 342, 532, 919, 931, 945, 1172, 1235
DpnII GATC 8 cut(s) 25, 340, 530, 917, 929, 943, 1170, 1233
EciI GGCGGA 2 cut(s) 1442, 1455
Eco130I CCWWGG 2 cut(s) 280, 1095
Eco31I GGTCTC 1 cut(s) 167
Eco32I GATATC 1 cut(s) 461
Eco47I GGWCC 4 cut(s) 201, 218, 738, 1082
Eco88I CYCGRG 1 cut(s) 117
EcoO109I RGGNCCY 1 cut(s) 738
EcoRII CCWGG 2 cut(s) 1324, 1543
EcoRV GATATC 1 cut(s) 461
EcoT14I CCWWGG 2 cut(s) 280, 1095
ErhI CCWWGG 2 cut(s) 280, 1095
Esp3I CGTCTC 1 cut(s) 1338
FaeI CATG 5 cut(s) 38, 567, 660, 777, 1304
FalI AAGNNNNNCTT 2 cut(s) 996, 1028
FaqI GGGAC 1 cut(s) 724
FatI CATG 5 cut(s) 34, 563, 656, 773, 1300
Fnu4HI GCNGC 5 cut(s) 81, 523, 1214, 1425, 1457
FokI GGATG 4 cut(s) 238, 263, 512, 774
Fsp4HI GCNGC 5 cut(s) 81, 523, 1214, 1425, 1457
FspBI CTAG 3 cut(s) 336, 509, 941
GluI GCNGC 5 cut(s) 81, 523, 1214, 1425, 1457
HaeIII GGCC 4 cut(s) 474, 1489, 1615, 1624
HapII CCGG 3 cut(s) 267, 426, 1185
HgaI GACGC 1 cut(s) 1171
Hin1II CATG 5 cut(s) 38, 567, 660, 777, 1304
HincII GTYRAC 1 cut(s) 589
HindII GTYRAC 1 cut(s) 589
HindIII AAGCTT 1 cut(s) 53
HinfI GANTC 8 cut(s) 398, 585, 636, 866, 887, 975, 1128, 1585
HpaII CCGG 3 cut(s) 267, 426, 1185
HphI GGTGA 6 cut(s) 90, 134, 1174, 1429, 1616, 1661
Hpy166II GTNNAC 3 cut(s) 204, 589, 1009
Hpy188I TCNGA 7 cut(s) 25, 233, 530, 535, 1034, 1133, 1320
Hpy188III TCNNGA 9 cut(s) 35, 164, 452, 509, 791, 1060, 1477, 1589, 1633
Hpy8I GTNNAC 3 cut(s) 204, 589, 1009
Hpy99I CGWCG 2 cut(s) 77, 1165
HpyAV CCTTC 6 cut(s) 105, 206, 273, 819, 1022, 1381
HpyCH4III ACNGT 4 cut(s) 102, 308, 896, 1119
HpyCH4V TGCA 3 cut(s) 83, 525, 1339
HpyF10VI GCNNNNNNNGC 1 cut(s) 1621
HpyF3I CTNAG 1 cut(s) 151
Hsp92II CATG 5 cut(s) 38, 567, 660, 777, 1304
KflI GGGWCCC 1 cut(s) 738
Kzo9I GATC 8 cut(s) 25, 340, 530, 917, 929, 943, 1170, 1233
LmnI GCTCC 4 cut(s) 233, 839, 1034, 1453
Lsp1109I GCAGC 3 cut(s) 67, 509, 1225
LweI GCATC 4 cut(s) 67, 534, 1228, 1301
MaeI CTAG 3 cut(s) 336, 509, 941
MaeIII GTNAC 4 cut(s) 102, 399, 481, 817
MalI GATC 8 cut(s) 27, 342, 532, 919, 931, 945, 1172, 1235
MboI GATC 8 cut(s) 25, 340, 530, 917, 929, 943, 1170, 1233
MflI RGATCY 2 cut(s) 340, 943
MhlI GDGCHC 1 cut(s) 578
MluCI AATT 7 cut(s) 408, 752, 1103, 1177, 1227, 1571, 1639
MlyI GAGTC 4 cut(s) 407, 594, 645, 984
MseI TTAA 7 cut(s) 359, 411, 539, 698, 1467, 1521, 1569
MslI CAYNNNNRTG 5 cut(s) 39, 568, 655, 1004, 1221
MspI CCGG 3 cut(s) 267, 426, 1185
MspR9I CCNGG 2 cut(s) 1326, 1545
Mva1269I GAATGC 1 cut(s) 83
MvaI CCWGG 2 cut(s) 1326, 1545
MvnI CGCG 1 cut(s) 1310
MwoI GCNNNNNNNGC 1 cut(s) 1621
NdeII GATC 8 cut(s) 25, 340, 530, 917, 929, 943, 1170, 1233
NlaIII CATG 5 cut(s) 38, 567, 660, 777, 1304
NlaIV GGNNCC 6 cut(s) 142, 424, 739, 740, 1455, 1488
NmuCI GTSAC 3 cut(s) 399, 481, 817
OliI CACNNNNGTG 1 cut(s) 1004
PaeR7I CTCGAG 1 cut(s) 117
PagI TCATGA 1 cut(s) 34
PcsI WCGNNNNNNNCGW 2 cut(s) 526, 1242
PctI GAATGC 1 cut(s) 83
PfeI GAWTC 4 cut(s) 866, 887, 1128, 1585
Pfl23II CGTACG 1 cut(s) 1287
PkrI GCNGC 5 cut(s) 82, 524, 1215, 1426, 1458
PleI GAGTC 4 cut(s) 406, 593, 644, 983
PpsI GAGTC 4 cut(s) 406, 593, 644, 983
PpuMI RGGWCCY 1 cut(s) 738
PsiI TTATAA 1 cut(s) 1503
Psp5II RGGWCCY 1 cut(s) 738
Psp6I CCWGG 2 cut(s) 1324, 1543
PspGI CCWGG 2 cut(s) 1324, 1543
PspLI CGTACG 1 cut(s) 1287
PspN4I GGNNCC 6 cut(s) 142, 424, 739, 740, 1455, 1488
PspPI GGNCC 7 cut(s) 201, 218, 738, 1082, 1487, 1613, 1622
PspPPI RGGWCCY 1 cut(s) 738
PspXI VCTCGAGB 1 cut(s) 117
PsrI GAACNNNNNNTAC 2 cut(s) 613, 645
PsuI RGATCY 2 cut(s) 340, 943
RsaI GTAC 5 cut(s) 327, 649, 827, 1010, 1289
RsaNI GTAC 5 cut(s) 326, 648, 826, 1009, 1288
RseI CAYNNNNRTG 5 cut(s) 39, 568, 655, 1004, 1221
SaqAI TTAA 7 cut(s) 359, 411, 539, 698, 1467, 1521, 1569
SatI GCNGC 5 cut(s) 81, 523, 1214, 1425, 1457
Sau3AI GATC 8 cut(s) 25, 340, 530, 917, 929, 943, 1170, 1233
Sau96I GGNCC 7 cut(s) 201, 218, 738, 1082, 1487, 1613, 1622
SchI GAGTC 4 cut(s) 407, 594, 645, 984
ScrFI CCNGG 2 cut(s) 1326, 1545
SduI GDGCHC 1 cut(s) 578
SfaNI GCATC 4 cut(s) 67, 534, 1228, 1301
SfcI CTRYAG 2 cut(s) 630, 951
SfiI GGCCNNNNNGGCC 1 cut(s) 1621
Sfr274I CTCGAG 1 cut(s) 117
SinI GGWCC 4 cut(s) 201, 218, 738, 1082
SlaI CTCGAG 1 cut(s) 117
SmiMI CAYNNNNRTG 5 cut(s) 39, 568, 655, 1004, 1221
SmlI CTYRAG 3 cut(s) 117, 346, 1631
SmoI CTYRAG 3 cut(s) 117, 346, 1631
Sse9I AATT 7 cut(s) 408, 752, 1103, 1177, 1227, 1571, 1639
SsiI CCGC 4 cut(s) 1424, 1427, 1440, 1457
SspMI CTAG 3 cut(s) 336, 509, 941
StyD4I CCNGG 2 cut(s) 1324, 1543
StyI CCWWGG 2 cut(s) 280, 1095
TaaI ACNGT 4 cut(s) 102, 308, 896, 1119
TaqI TCGA 9 cut(s) 75, 118, 165, 182, 714, 747, 869, 885, 1236
TasI AATT 7 cut(s) 408, 752, 1103, 1177, 1227, 1571, 1639
TauI GCSGC 2 cut(s) 1427, 1459
TfiI GAWTC 4 cut(s) 866, 887, 1128, 1585
Tru1I TTAA 7 cut(s) 359, 411, 539, 698, 1467, 1521, 1569
Tru9I TTAA 7 cut(s) 359, 411, 539, 698, 1467, 1521, 1569
TseFI GTSAC 3 cut(s) 399, 481, 817
TseI GCWGC 3 cut(s) 80, 522, 1213
Tsp45I GTSAC 3 cut(s) 399, 481, 817
TspDTI ATGAA 5 cut(s) 51, 225, 1004, 1530, 1608
TspGWI ACGGA 5 cut(s) 435, 597, 852, 1183, 1278
VpaK11BI GGWCC 4 cut(s) 201, 218, 738, 1082
XapI RAATTY 3 cut(s) 1103, 1227, 1571
XbaI TCTAGA 1 cut(s) 508
XhoI CTCGAG 1 cut(s) 117
XspI CTAG 3 cut(s) 336, 509, 941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.