RLG00000030531

Vacuolar protein sorting-associated protein 62

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0009493
Physical Location & Seq
Reverse (-)
63076057 .. 63077965
1909 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030531

Sequence Viewer

Length: 1680 bp
ATGGGAAATTGCCTTTCCCTCTCATGTTCAACCAAGAACATCAAGGCGAATCGGATCGAAAAGAATCCGATCGAAACCACATTCAAGCTTCCCTCTCCATTACCCAGTTGGCCATCTGGTAATGGGTTCGCAAATGGAATCATAAATCTGGGAGGATTACAAGTATGCCAAATTTCATCTTTCAACAAAGTATGGGCAACCCATGAGGGAGGACCAGGCAACCTTGGGGCAACCTTCTTCGAACCTTCACCCCTACCCCAAGGATTCTATATGCTCGGTTGCTACAGCCAACCCAACAACAAACCCCTTTTCGGATGGGCCCTTGCTGCCAAAGCCGACGATCACTCCTCCTCTCCATGGCCGGCTTTACAGCAACCAGTTGATTACACTCTGGTATGGAGCAGCGAGTCCGTGAAGATGAAGAAAGATGGTGATGGATACCTCTGGTTACCCACTGCCCCGGAGCGTTATGCAGCCATAGGCCATGTTGTCACTAGCTCTCCTGAGAAGCCGTCCCTTGATAAAATCAGGTGCGTTCGATCTGACCTTACTGACCAATGTGAAGCTGACTCGTGGATATGGGGACCCCCTGGTGGCACCATTGATGGGTTCAATGTATATAGTCTCACACCCAGCAATAGGGGCATCCAAGCTATGGGTGTCCCCGTTGGCACATTCCTAGTCAGTAATGGTAAAGCAAATAATATAGCTTGTTTGAAAAATAATAACACCCATCCACACACTTGTATGCCAAACCAAAGCCAAATTGATGCAATACTTCAAGCTTACGCTCCATTCATATACTTCCATCCCGATGAAGTTTACCTCCCTTCTTCGGTGGGTTGGTTTTTCACAAATGGAGCACTTCTGTATAGCAAGGAACAAGTAGAAAATCCCGTTCCAATTGAACCCACTGGCTCTAACCTTCCGCAAGGTGGCTCAAATGATGGCGCCTATTGGTTGGACCTACCGGTCGATCAAGCCGCCAAAGAAAGGGTGAAAAAGGGAGATCTTGTTGACGCCGAGGCCTACTTACACATAAAACCCATGTTGGGCTCAACATTCACTGACATAGCTATTTGGGTCTTCTACCCTTTCAATGGCCCCGCTAGGGCCAAAGTTGAGTTCTTCGATGTTTCGTTGGGGAAGATAGGTGAACATGTTGGTGACTGGGAGCATGTCACATTAAGAGTCAGCAATTTCACTGGCCAACTCTGGAGTGTCTACTTCTCGGAACACAGTGGGGGAATATGGGTGGACGCTTCTGGGCTTGAATTTCAAAATGGCAACAAACCTGTGACATATGCATCTTTGCATGGACATGCCTTCTATTCCAAACCAGGACTTGTTTTGCAAGGGAATGGAAGCATAGGAATCAGGAATGATACTGCAAAGAGTAAAATGGTAATGGACTCTGGACAAAGGCCTTCAATAATTGCAGCCGACTATCTGGGCGCGGCTATCATTGAACCACCATGGCTTAACTACAGCAGGAAATGGGGTCCGAAAATTAGTTATCAGATTTCGGATGAAATAAAAAAGGTGGAGAAGCTCTTGCCTGGAAAGCTCAAGACTGTTTTTGACAAGTTTATCAAAAGTCTACCAGATGAAGTATTAGGAGAAGAAGGGCCTACAGGTCCCAACATGAAGAATAACTGGAATGGAGATGAAGTTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps62

Protein Analysis

560

Amino Acids

61.16

Weight (kDa)

5.88

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps62 PF06101 25 - 557 1.2e-253 Vacuolar protein sorting-associated protein 62
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 971
AccB1I GGYRCC 2 cut(s) 596, 950
AccB7I CCANNNNNTGG 1 cut(s) 655
AccI GTMKAC 2 cut(s) 1224, 1600
AccII CGCG 1 cut(s) 1457
AciI CCGC 4 cut(s) 929, 984, 1107, 1457
AclWI GGATC 1 cut(s) 62
AcoI YGGCCR 3 cut(s) 110, 359, 1207
AcsI RAATTY 2 cut(s) 171, 1274
AcyI GRCGYC 2 cut(s) 951, 1020
AflIII ACRYGT 1 cut(s) 1159
AgeI ACCGGT 1 cut(s) 970
AjnI CCWGG 4 cut(s) 214, 589, 1339, 1558
AjuI GAANNNNNNNTTGG 2 cut(s) 1109, 1141
AluBI AGCT 9 cut(s) 88, 498, 566, 653, 710, 785, 1076, 1552, 1567
AluI AGCT 9 cut(s) 88, 498, 566, 653, 710, 785, 1076, 1552, 1567
Alw21I GWGCWC 1 cut(s) 865
Alw26I GTCTC 1 cut(s) 629
AlwI GGATC 1 cut(s) 62
ApaI GGGCCC 1 cut(s) 322
ApeKI GCWGC 4 cut(s) 326, 402, 473, 1439
ApoI RAATTY 2 cut(s) 171, 1274
AsiGI ACCGGT 1 cut(s) 970
AspLEI GCGC 2 cut(s) 953, 1457
AsuC2I CCSGG 1 cut(s) 461
AsuHPI GGTGA 5 cut(s) 240, 443, 1009, 1166, 1178
AsuII TTCGAA 1 cut(s) 240
AvaII GGWCC 5 cut(s) 212, 584, 964, 1502, 1637
BaeGI GKGCMC 1 cut(s) 322
BalI TGGCCA 2 cut(s) 112, 1209
BanI GGYRCC 2 cut(s) 596, 950
BanII GRGCYC 2 cut(s) 322, 1058
BauI CACGAG 1 cut(s) 571
BbsI GAAGAC 1 cut(s) 1078
Bbv12I GWGCWC 1 cut(s) 865
BbvI GCAGC 4 cut(s) 313, 414, 485, 1451
BccI CCATC 8 cut(s) 121, 309, 422, 428, 599, 741, 816, 941
BceAI ACGGC 1 cut(s) 496
BciT130I CCWGG 4 cut(s) 216, 591, 1341, 1560
BciVI GTATCC 1 cut(s) 431
BcnI CCSGG 1 cut(s) 461
BcoDI GTCTC 1 cut(s) 629
BfaI CTAG 3 cut(s) 495, 680, 1110
BfmI CTRYAG 3 cut(s) 283, 1486, 1632
BfoI RGCGCY 1 cut(s) 954
BfuI GTATCC 1 cut(s) 431
BglII AGATCT 1 cut(s) 1009
BisI GCNGC 6 cut(s) 327, 403, 474, 984, 1440, 1458
BlsI GCNGC 6 cut(s) 328, 404, 475, 985, 1441, 1459
Bme1390I CCNGG 5 cut(s) 216, 461, 591, 1341, 1560
Bme18I GGWCC 5 cut(s) 212, 584, 964, 1502, 1637
BmiI GGNNCC 8 cut(s) 320, 585, 586, 598, 952, 1105, 1503, 1639
BmrFI CCNGG 5 cut(s) 216, 461, 591, 1341, 1560
BmrI ACTGGG 2 cut(s) 99, 1180
BmsI GCATC 3 cut(s) 654, 760, 1316
BmuI ACTGGG 2 cut(s) 99, 1180
BpiI GAAGAC 1 cut(s) 1078
BpmI CTGGAG 1 cut(s) 1237
Bpu14I TTCGAA 1 cut(s) 240
BpuEI CTTGAG 1 cut(s) 1553
BpuMI CCSGG 1 cut(s) 461
BsaHI GRCGYC 2 cut(s) 951, 1020
BsaJI CCNNGG 7 cut(s) 223, 259, 356, 459, 589, 1023, 1475
BsaWI WCCGGW 1 cut(s) 970
BsaXI ACNNNNNCTCC 7 cut(s) 329, 359, 484, 514, 999, 1029, 1656
Bse118I RCCGGY 2 cut(s) 361, 970
Bse1I ACTGG 6 cut(s) 105, 377, 919, 1175, 1210, 1661
BseBI CCWGG 4 cut(s) 216, 591, 1341, 1560
BseDI CCNNGG 7 cut(s) 223, 259, 356, 459, 589, 1023, 1475
BseGI GGATG 5 cut(s) 320, 645, 733, 808, 1534
BseMII CTCAG 1 cut(s) 495
BseNI ACTGG 6 cut(s) 105, 377, 919, 1175, 1210, 1661
BseRI GAGGAG 2 cut(s) 337, 340
BseSI GKGCMC 1 cut(s) 322
BseXI GCAGC 4 cut(s) 313, 414, 485, 1451
BseYI CCCAGC 1 cut(s) 632
Bsh1236I CGCG 1 cut(s) 1457
Bsh1285I CGRYCG 2 cut(s) 72, 975
BshNI GGYRCC 2 cut(s) 596, 950
BshTI ACCGGT 1 cut(s) 970
BsiEI CGRYCG 2 cut(s) 72, 975
BsiHKAI GWGCWC 1 cut(s) 865
BsiSI CCGG 3 cut(s) 362, 461, 971
BslFI GGGAC 4 cut(s) 499, 597, 647, 1623
BsmAI GTCTC 1 cut(s) 629
BsmFI GGGAC 4 cut(s) 499, 597, 647, 1623
Bsp119I TTCGAA 1 cut(s) 240
Bsp120I GGGCCC 1 cut(s) 318
Bsp1286I GDGCHC 3 cut(s) 322, 865, 1058
Bsp143I GATC 6 cut(s) 54, 69, 340, 539, 976, 1009
Bsp19I CCATGG 2 cut(s) 356, 1475
BspACI CCGC 4 cut(s) 929, 984, 1107, 1457
BspCNI CTCAG 1 cut(s) 496
BspFNI CGCG 1 cut(s) 1457
BspLI GGNNCC 8 cut(s) 320, 585, 586, 598, 952, 1105, 1503, 1639
BspPI GGATC 1 cut(s) 62
BspT104I TTCGAA 1 cut(s) 240
BspT107I GGYRCC 2 cut(s) 596, 950
BsrFI RCCGGY 2 cut(s) 361, 970
BsrI ACTGG 6 cut(s) 105, 377, 919, 1175, 1210, 1661
BssAI RCCGGY 2 cut(s) 361, 970
BssECI CCNNGG 7 cut(s) 223, 259, 356, 459, 589, 1023, 1475
BssMI GATC 6 cut(s) 54, 69, 340, 539, 976, 1009
BssNI GRCGYC 2 cut(s) 951, 1020
BssSI CACGAG 1 cut(s) 571
BssT1I CCWWGG 4 cut(s) 223, 259, 356, 1475
Bst2BI CACGAG 1 cut(s) 571
Bst2UI CCWGG 4 cut(s) 216, 591, 1341, 1560
Bst4CI ACNGT 2 cut(s) 1241, 1576
BstACI GRCGYC 2 cut(s) 951, 1020
BstBI TTCGAA 1 cut(s) 240
BstC8I GCNNGC 1 cut(s) 363
BstDEI CTNAG 1 cut(s) 504
BstDSI CCRYGG 2 cut(s) 356, 1475
BstEII GGTNACC 1 cut(s) 447
BstF5I GGATG 5 cut(s) 320, 645, 733, 808, 1534
BstFNI CGCG 1 cut(s) 1457
BstH2I RGCGCY 1 cut(s) 954
BstHHI GCGC 2 cut(s) 953, 1457
BstKTI GATC 6 cut(s) 57, 72, 343, 542, 979, 1012
BstMAI GTCTC 1 cut(s) 629
BstMBI GATC 6 cut(s) 54, 69, 340, 539, 976, 1009
BstMCI CGRYCG 2 cut(s) 72, 975
BstMWI GCNNNNNNNGC 4 cut(s) 326, 332, 642, 1564
BstNI CCWGG 4 cut(s) 216, 591, 1341, 1560
BstNSI RCATGY 3 cut(s) 1163, 1181, 1325
BstPI GGTNACC 1 cut(s) 447
BstSCI CCNGG 5 cut(s) 214, 459, 589, 1339, 1558
BstSFI CTRYAG 3 cut(s) 283, 1486, 1632
BstSLI GKGCMC 1 cut(s) 322
BstUI CGCG 1 cut(s) 1457
BstV1I GCAGC 4 cut(s) 313, 414, 485, 1451
BstV2I GAAGAC 1 cut(s) 1078
BstX2I RGATCY 1 cut(s) 1009
BstYI RGATCY 1 cut(s) 1009
BsuI GTATCC 1 cut(s) 431
BtgI CCRYGG 2 cut(s) 356, 1475
BtsCI GGATG 5 cut(s) 320, 645, 733, 808, 1534
BtsI GCAGTG 1 cut(s) 453
BtsIMutI CAGTG 5 cut(s) 453, 912, 1065, 1203, 1246
Cac8I GCNNGC 1 cut(s) 363
CfoI GCGC 2 cut(s) 953, 1457
Cfr10I RCCGGY 2 cut(s) 361, 970
CseI GACGC 2 cut(s) 1028, 1268
CspAI ACCGGT 1 cut(s) 970
DdeI CTNAG 1 cut(s) 504
DinI GGCGCC 1 cut(s) 952
DpnI GATC 6 cut(s) 56, 71, 342, 541, 978, 1011
DpnII GATC 6 cut(s) 54, 69, 340, 539, 976, 1009
DrdI GACNNNNNNGTC 1 cut(s) 971
DseDI GACNNNNNNGTC 1 cut(s) 971
EaeI YGGCCR 3 cut(s) 110, 359, 1207
Eco130I CCWWGG 4 cut(s) 223, 259, 356, 1475
Eco147I AGGCCT 2 cut(s) 1028, 1426
Eco24I GRGCYC 2 cut(s) 322, 1058
Eco47I GGWCC 5 cut(s) 212, 584, 964, 1502, 1637
Eco91I GGTNACC 1 cut(s) 447
EcoO109I RGGNCCY 4 cut(s) 319, 584, 1628, 1637
EcoO65I GGTNACC 1 cut(s) 447
EcoRII CCWGG 4 cut(s) 214, 589, 1339, 1558
EcoT14I CCWWGG 4 cut(s) 223, 259, 356, 1475
EcoT22I ATGCAT 1 cut(s) 1309
EcoT38I GRGCYC 2 cut(s) 322, 1058
EgeI GGCGCC 1 cut(s) 952
EheI GGCGCC 1 cut(s) 952
ErhI CCWWGG 4 cut(s) 223, 259, 356, 1475
FaqI GGGAC 4 cut(s) 499, 597, 647, 1623
FauI CCCGC 1 cut(s) 1114
FauNDI CATATG 1 cut(s) 1303
FblI GTMKAC 2 cut(s) 1224, 1600
Fnu4HI GCNGC 6 cut(s) 327, 403, 474, 984, 1440, 1458
FokI GGATG 5 cut(s) 327, 632, 720, 795, 1541
FriOI GRGCYC 2 cut(s) 322, 1058
Fsp4HI GCNGC 6 cut(s) 327, 403, 474, 984, 1440, 1458
FspBI CTAG 3 cut(s) 495, 680, 1110
GlaI GCGC 2 cut(s) 952, 1456
GluI GCNGC 6 cut(s) 327, 403, 474, 984, 1440, 1458
GsaI CCCAGC 1 cut(s) 636
GsuI CTGGAG 1 cut(s) 1237
HaeII RGCGCY 1 cut(s) 954
HapII CCGG 3 cut(s) 362, 461, 971
HgaI GACGC 2 cut(s) 1028, 1268
HhaI GCGC 2 cut(s) 953, 1457
Hin1I GRCGYC 2 cut(s) 951, 1020
Hin6I GCGC 2 cut(s) 951, 1455
HinP1I GCGC 2 cut(s) 951, 1455
HincII GTYRAC 1 cut(s) 1018
HindII GTYRAC 1 cut(s) 1018
HindIII AAGCTT 2 cut(s) 86, 783
HinfI GANTC 9 cut(s) 49, 64, 138, 264, 407, 569, 1191, 1374, 1412
HpaII CCGG 3 cut(s) 362, 461, 971
HphI GGTGA 5 cut(s) 240, 443, 1009, 1166, 1178
Hpy166II GTNNAC 6 cut(s) 823, 1018, 1157, 1225, 1258, 1601
Hpy188I TCNGA 8 cut(s) 54, 69, 314, 544, 1234, 1506, 1521, 1528
Hpy188III TCNNGA 6 cut(s) 503, 812, 1216, 1378, 1416, 1570
Hpy8I GTNNAC 6 cut(s) 823, 1018, 1157, 1225, 1258, 1601
Hpy99I CGWCG 1 cut(s) 341
HpyAV CCTTC 7 cut(s) 244, 255, 840, 935, 1336, 1437, 1619
HpyCH4III ACNGT 2 cut(s) 1241, 1576
HpyCH4V TGCA 7 cut(s) 473, 773, 1307, 1315, 1354, 1391, 1439
HpyF10VI GCNNNNNNNGC 4 cut(s) 326, 332, 642, 1564
HpyF3I CTNAG 1 cut(s) 504
Hsp92I GRCGYC 2 cut(s) 951, 1020
HspAI GCGC 2 cut(s) 951, 1455
KasI GGCGCC 1 cut(s) 950
KflI GGGWCCC 1 cut(s) 584
KroI GCCGGC 1 cut(s) 361
KroNI GCCGGC 1 cut(s) 363
Kzo9I GATC 6 cut(s) 54, 69, 340, 539, 976, 1009
LmnI GCTCC 5 cut(s) 399, 463, 796, 860, 1174
Lsp1109I GCAGC 4 cut(s) 313, 414, 485, 1451
LweI GCATC 3 cut(s) 654, 760, 1316
MaeI CTAG 3 cut(s) 495, 680, 1110
MaeIII GTNAC 5 cut(s) 447, 490, 1166, 1180, 1297
MalI GATC 6 cut(s) 56, 71, 342, 541, 978, 1011
MboI GATC 6 cut(s) 54, 69, 340, 539, 976, 1009
MboII GAAGA 9 cut(s) 229, 427, 433, 825, 1078, 1120, 1159, 1634, 1660
MfeI CAATTG 1 cut(s) 903
MflI RGATCY 1 cut(s) 1009
MhlI GDGCHC 3 cut(s) 322, 865, 1058
MlsI TGGCCA 2 cut(s) 112, 1209
MluCI AATT 8 cut(s) 7, 171, 765, 903, 1198, 1274, 1434, 1509
MluNI TGGCCA 2 cut(s) 112, 1209
Mly113I GGCGCC 1 cut(s) 951
MlyI GAGTC 4 cut(s) 416, 563, 1200, 1406
MmeI TCCRAC 1 cut(s) 942
Mox20I TGGCCA 2 cut(s) 112, 1209
Mph1103I ATGCAT 1 cut(s) 1309
MroNI GCCGGC 1 cut(s) 361
MscI TGGCCA 2 cut(s) 112, 1209
MseI TTAA 2 cut(s) 1187, 1482
MslI CAYNNNNRTG 4 cut(s) 746, 813, 1164, 1320
Msp20I TGGCCA 2 cut(s) 112, 1209
MspI CCGG 3 cut(s) 362, 461, 971
MspR9I CCNGG 5 cut(s) 216, 461, 591, 1341, 1560
MunI CAATTG 1 cut(s) 903
MvaI CCWGG 4 cut(s) 216, 591, 1341, 1560
MvnI CGCG 1 cut(s) 1457
MwoI GCNNNNNNNGC 4 cut(s) 326, 332, 642, 1564
NaeI GCCGGC 1 cut(s) 363
NarI GGCGCC 1 cut(s) 951
NciI CCSGG 1 cut(s) 461
NcoI CCATGG 2 cut(s) 356, 1475
NdeI CATATG 1 cut(s) 1303
NdeII GATC 6 cut(s) 54, 69, 340, 539, 976, 1009
NgoMIV GCCGGC 1 cut(s) 361
NlaIV GGNNCC 8 cut(s) 320, 585, 586, 598, 952, 1105, 1503, 1639
NmeAIII GCCGAG 1 cut(s) 1048
NmuCI GTSAC 4 cut(s) 490, 1166, 1180, 1297
NsiI ATGCAT 1 cut(s) 1309
NspI RCATGY 3 cut(s) 1163, 1181, 1325
NspV TTCGAA 1 cut(s) 240
PceI AGGCCT 2 cut(s) 1028, 1426
PciI ACATGT 1 cut(s) 1159
PdiI GCCGGC 1 cut(s) 363
PfeI GAWTC 5 cut(s) 49, 64, 138, 264, 1374
PflMI CCANNNNNTGG 1 cut(s) 655
PinAI ACCGGT 1 cut(s) 970
PkrI GCNGC 6 cut(s) 328, 404, 475, 985, 1441, 1459
Ple19I CGATCG 1 cut(s) 72
PleI GAGTC 4 cut(s) 415, 563, 1199, 1406
PluTI GGCGCC 1 cut(s) 954
PpsI GAGTC 4 cut(s) 415, 563, 1199, 1406
PpuMI RGGWCCY 2 cut(s) 584, 1637
PscI ACATGT 1 cut(s) 1159
Psp5II RGGWCCY 2 cut(s) 584, 1637
Psp6I CCWGG 4 cut(s) 214, 589, 1339, 1558
PspEI GGTNACC 1 cut(s) 447
PspFI CCCAGC 1 cut(s) 632
PspGI CCWGG 4 cut(s) 214, 589, 1339, 1558
PspN4I GGNNCC 8 cut(s) 320, 585, 586, 598, 952, 1105, 1503, 1639
PspOMI GGGCCC 1 cut(s) 318
PspPPI RGGWCCY 2 cut(s) 584, 1637
PsuI RGATCY 1 cut(s) 1009
PvuI CGATCG 1 cut(s) 72
RseI CAYNNNNRTG 4 cut(s) 746, 813, 1164, 1320
SaqAI TTAA 2 cut(s) 1187, 1482
SatI GCNGC 6 cut(s) 327, 403, 474, 984, 1440, 1458
Sau3AI GATC 6 cut(s) 54, 69, 340, 539, 976, 1009
SchI GAGTC 4 cut(s) 416, 563, 1200, 1406
ScrFI CCNGG 5 cut(s) 216, 461, 591, 1341, 1560
SduI GDGCHC 3 cut(s) 322, 865, 1058
SfaNI GCATC 3 cut(s) 654, 760, 1316
SfcI CTRYAG 3 cut(s) 283, 1486, 1632
SfoI GGCGCC 1 cut(s) 952
SfuI TTCGAA 1 cut(s) 240
SinI GGWCC 5 cut(s) 212, 584, 964, 1502, 1637
SmiMI CAYNNNNRTG 4 cut(s) 746, 813, 1164, 1320
SmlI CTYRAG 1 cut(s) 1568
SmoI CTYRAG 1 cut(s) 1568
Sse9I AATT 8 cut(s) 7, 171, 765, 903, 1198, 1274, 1434, 1509
SseBI AGGCCT 2 cut(s) 1028, 1426
SsiI CCGC 4 cut(s) 929, 984, 1107, 1457
SspDI GGCGCC 1 cut(s) 950
SspMI CTAG 3 cut(s) 495, 680, 1110
StuI AGGCCT 2 cut(s) 1028, 1426
StyD4I CCNGG 5 cut(s) 214, 459, 589, 1339, 1558
StyI CCWWGG 4 cut(s) 223, 259, 356, 1475
TaaI ACNGT 2 cut(s) 1241, 1576
TaqI TCGA 6 cut(s) 57, 72, 240, 538, 975, 1131
TasI AATT 8 cut(s) 7, 171, 765, 903, 1198, 1274, 1434, 1509
TauI GCSGC 2 cut(s) 986, 1460
TfiI GAWTC 5 cut(s) 49, 64, 138, 264, 1374
Tru1I TTAA 2 cut(s) 1187, 1482
Tru9I TTAA 2 cut(s) 1187, 1482
TscAI CASTG 5 cut(s) 460, 919, 1072, 1210, 1246
TseFI GTSAC 4 cut(s) 490, 1166, 1180, 1297
TseI GCWGC 4 cut(s) 326, 402, 473, 1439
Tsp45I GTSAC 4 cut(s) 490, 1166, 1180, 1297
TspDTI ATGAA 7 cut(s) 165, 434, 787, 831, 1545, 1623, 1661
TspGWI ACGGA 1 cut(s) 400
TspRI CASTG 5 cut(s) 460, 919, 1072, 1210, 1246
Van91I CCANNNNNTGG 1 cut(s) 655
VpaK11BI GGWCC 5 cut(s) 212, 584, 964, 1502, 1637
XapI RAATTY 2 cut(s) 171, 1274
XceI RCATGY 3 cut(s) 1163, 1181, 1325
XcmI CCANNNNNNNNNTGG 1 cut(s) 105
XmiI GTMKAC 2 cut(s) 1224, 1600
XspI CTAG 3 cut(s) 495, 680, 1110
Zsp2I ATGCAT 1 cut(s) 1309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.