pycom07g00030

Vacuolar protein sorting-associated protein 62

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0009493
Physical Location & Seq
Forward (+)
21987 .. 22475
489 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g00030.1

Sequence Viewer

Length: 489 bp
ATGCTTGGCTGCTACAGCCAACCCAACAACATACCCCTTTACGGATGGGCCCTTGCTGCTAAACCGACAAAAGACAACGACGATGACCCTTCTTCGCCCTTGCTGCGGCAACCAGTTGATTTCACTCTCATTTGGAGTAGCGAGTTCTTGAACATCAAGAAAGATGGCAATGGCTATGTCTGGTTACCCACAGCCTCAGATGGTTACAAAGCCATAGGCCTTGTTGTCACAAGCTCTCCGGAGAAGCCATCCCTCGATAAAATCAGGTGCGTTCGTTCCAGCCTCACTGACGAGTGCGAGGCCGACTCGTGGATCTGGGGACCAGGTACCGCCAGCGATGCCAATGGATTCAAAGTCTTTAGCGTCAGACCCAGCAATAGGGGGACCTCAGCCATGGGTGTCTTCGCTGGCACTTTTATAGCTGCTCAACAAAATGGTGGGTTGTCAACTGTGGCTTGTTTGAAAAACGCCGCCATATCTAATCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps62

Protein Analysis

163

Amino Acids

17.31

Weight (kDa)

5.33

Isoelectric Point (pI)

39.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps62 PF06101 1 - 158 9.9e-53 Vacuolar protein sorting-associated protein 62
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 326
AccB1I GGYRCC 1 cut(s) 326
AccIII TCCGGA 1 cut(s) 238
AciI CCGC 3 cut(s) 106, 330, 471
AclWI GGATC 1 cut(s) 320
AfaI GTAC 1 cut(s) 328
AfiI CCNNNNNNNGG 4 cut(s) 41, 105, 309, 378
AgsI TTSAA 3 cut(s) 151, 352, 463
AjnI CCWGG 1 cut(s) 322
AluBI AGCT 2 cut(s) 234, 422
AluI AGCT 2 cut(s) 234, 422
AlwI GGATC 1 cut(s) 320
Aor13HI TCCGGA 1 cut(s) 238
AoxI GGCC 3 cut(s) 48, 217, 300
ApaI GGGCCC 1 cut(s) 52
ApeKI GCWGC 4 cut(s) 9, 56, 103, 422
Asp718I GGTACC 1 cut(s) 326
AspS9I GGNCC 4 cut(s) 48, 49, 320, 384
AvaII GGWCC 2 cut(s) 320, 384
BaeGI GKGCMC 1 cut(s) 52
BanI GGYRCC 1 cut(s) 326
BanII GRGCYC 1 cut(s) 52
BauI CACGAG 1 cut(s) 307
BbsI GAAGAC 1 cut(s) 394
BbvCI CCTCAGC 1 cut(s) 388
BbvI GCAGC 3 cut(s) 43, 90, 409
BccI CCATC 4 cut(s) 39, 158, 194, 256
BciT130I CCWGG 1 cut(s) 324
BfmI CTRYAG 1 cut(s) 13
BisI GCNGC 6 cut(s) 10, 57, 104, 107, 423, 471
BlsI GCNGC 6 cut(s) 11, 58, 105, 108, 424, 472
Bme1390I CCNGG 1 cut(s) 324
Bme18I GGWCC 2 cut(s) 320, 384
BmgT120I GGNCC 4 cut(s) 48, 49, 320, 384
BmiI GGNNCC 4 cut(s) 50, 321, 328, 385
BmrFI CCNGG 1 cut(s) 324
BmsI GCATC 1 cut(s) 328
BpiI GAAGAC 1 cut(s) 394
BplI GAGNNNNNCTC 2 cut(s) 290, 322
Bpu10I CCTNAGC 1 cut(s) 388
BsaJI CCNNGG 1 cut(s) 393
BsaWI WCCGGW 1 cut(s) 238
BsaXI ACNNNNNCTCC 2 cut(s) 220, 250
Bsc4I CCNNNNNNNGG 4 cut(s) 41, 105, 309, 378
Bse1I ACTGG 1 cut(s) 113
Bse3DI GCAATG 1 cut(s) 175
BseAI TCCGGA 1 cut(s) 238
BseBI CCWGG 1 cut(s) 324
BseDI CCNNGG 1 cut(s) 393
BseGI GGATG 2 cut(s) 50, 248
BseLI CCNNNNNNNGG 4 cut(s) 41, 105, 309, 378
BseMI GCAATG 1 cut(s) 175
BseMII CTCAG 2 cut(s) 210, 402
BseNI ACTGG 1 cut(s) 113
BseSI GKGCMC 1 cut(s) 52
BseXI GCAGC 3 cut(s) 43, 90, 409
BseYI CCCAGC 1 cut(s) 371
BshFI GGCC 3 cut(s) 50, 219, 302
BshNI GGYRCC 1 cut(s) 326
BsiSI CCGG 1 cut(s) 239
BslFI GGGAC 2 cut(s) 333, 397
BslI CCNNNNNNNGG 4 cut(s) 41, 105, 309, 378
BsmFI GGGAC 2 cut(s) 333, 397
BsnI GGCC 3 cut(s) 50, 219, 302
Bsp120I GGGCCC 1 cut(s) 48
Bsp1286I GDGCHC 1 cut(s) 52
Bsp13I TCCGGA 1 cut(s) 238
Bsp143I GATC 1 cut(s) 312
Bsp19I CCATGG 1 cut(s) 393
BspACI CCGC 3 cut(s) 106, 330, 471
BspANI GGCC 3 cut(s) 50, 219, 302
BspCNI CTCAG 2 cut(s) 209, 401
BspEI TCCGGA 1 cut(s) 238
BspLI GGNNCC 4 cut(s) 50, 321, 328, 385
BspPI GGATC 1 cut(s) 320
BspT107I GGYRCC 1 cut(s) 326
BsrDI GCAATG 1 cut(s) 175
BsrI ACTGG 1 cut(s) 113
BssECI CCNNGG 1 cut(s) 393
BssMI GATC 1 cut(s) 312
BssSI CACGAG 1 cut(s) 307
BssT1I CCWWGG 1 cut(s) 393
Bst2BI CACGAG 1 cut(s) 307
Bst2UI CCWGG 1 cut(s) 324
Bst4CI ACNGT 1 cut(s) 451
BstC8I GCNNGC 2 cut(s) 334, 409
BstDEI CTNAG 2 cut(s) 196, 388
BstDSI CCRYGG 1 cut(s) 393
BstEII GGTNACC 1 cut(s) 183
BstF5I GGATG 2 cut(s) 50, 248
BstKTI GATC 1 cut(s) 315
BstMBI GATC 1 cut(s) 312
BstMWI GCNNNNNNNGC 4 cut(s) 15, 56, 103, 338
BstNI CCWGG 1 cut(s) 324
BstPI GGTNACC 1 cut(s) 183
BstSCI CCNGG 1 cut(s) 322
BstSFI CTRYAG 1 cut(s) 13
BstSLI GKGCMC 1 cut(s) 52
BstV1I GCAGC 3 cut(s) 43, 90, 409
BstV2I GAAGAC 1 cut(s) 394
BstX2I RGATCY 1 cut(s) 312
BstYI RGATCY 1 cut(s) 312
BsuRI GGCC 3 cut(s) 50, 219, 302
BtgI CCRYGG 1 cut(s) 393
BtgZI GCGATG 1 cut(s) 351
BtsCI GGATG 2 cut(s) 50, 248
BtsIMutI CAGTG 1 cut(s) 285
Cac8I GCNNGC 2 cut(s) 334, 409
Cfr13I GGNCC 4 cut(s) 48, 49, 320, 384
CseI GACGC 1 cut(s) 352
CsiI ACCWGGT 1 cut(s) 322
Csp6I GTAC 1 cut(s) 327
CviAII CATG 1 cut(s) 394
CviQI GTAC 1 cut(s) 327
DdeI CTNAG 2 cut(s) 196, 388
DpnI GATC 1 cut(s) 314
DpnII GATC 1 cut(s) 312
Eco130I CCWWGG 1 cut(s) 393
Eco147I AGGCCT 1 cut(s) 219
Eco24I GRGCYC 1 cut(s) 52
Eco47I GGWCC 2 cut(s) 320, 384
Eco91I GGTNACC 1 cut(s) 183
EcoO109I RGGNCCY 2 cut(s) 49, 384
EcoO65I GGTNACC 1 cut(s) 183
EcoRII CCWGG 1 cut(s) 322
EcoT14I CCWWGG 1 cut(s) 393
EcoT38I GRGCYC 1 cut(s) 52
ErhI CCWWGG 1 cut(s) 393
FaeI CATG 1 cut(s) 397
FaiI YATR 6 cut(s) 32, 177, 215, 395, 419, 476
FaqI GGGAC 2 cut(s) 333, 397
FatI CATG 1 cut(s) 393
Fnu4HI GCNGC 6 cut(s) 10, 57, 104, 107, 423, 471
FokI GGATG 2 cut(s) 57, 235
FriOI GRGCYC 1 cut(s) 52
Fsp4HI GCNGC 6 cut(s) 10, 57, 104, 107, 423, 471
GluI GCNGC 6 cut(s) 10, 57, 104, 107, 423, 471
GsaI CCCAGC 1 cut(s) 375
HaeIII GGCC 3 cut(s) 50, 219, 302
HapII CCGG 1 cut(s) 239
HgaI GACGC 1 cut(s) 352
Hin1II CATG 1 cut(s) 397
HincII GTYRAC 1 cut(s) 447
HindII GTYRAC 1 cut(s) 447
HinfI GANTC 2 cut(s) 305, 348
HpaII CCGG 1 cut(s) 239
Hpy166II GTNNAC 1 cut(s) 447
Hpy188I TCNGA 2 cut(s) 199, 368
Hpy188III TCNNGA 3 cut(s) 148, 157, 239
Hpy8I GTNNAC 1 cut(s) 447
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 1 cut(s) 99
HpyCH4III ACNGT 1 cut(s) 451
HpyF10VI GCNNNNNNNGC 4 cut(s) 15, 56, 103, 338
HpyF3I CTNAG 2 cut(s) 196, 388
Hsp92II CATG 1 cut(s) 397
Kpn2I TCCGGA 1 cut(s) 238
KpnI GGTACC 1 cut(s) 330
Kzo9I GATC 1 cut(s) 312
Lsp1109I GCAGC 3 cut(s) 43, 90, 409
LweI GCATC 1 cut(s) 328
MabI ACCWGGT 1 cut(s) 322
MaeIII GTNAC 3 cut(s) 183, 203, 226
MalI GATC 1 cut(s) 314
MboI GATC 1 cut(s) 312
MboII GAAGA 2 cut(s) 84, 394
MflI RGATCY 1 cut(s) 312
MhlI GDGCHC 1 cut(s) 52
MlyI GAGTC 1 cut(s) 299
MnlI CCTC 5 cut(s) 205, 263, 292, 293, 397
MroI TCCGGA 1 cut(s) 238
MspI CCGG 1 cut(s) 239
MspR9I CCNGG 1 cut(s) 324
MvaI CCWGG 1 cut(s) 324
MwoI GCNNNNNNNGC 4 cut(s) 15, 56, 103, 338
NcoI CCATGG 1 cut(s) 393
NdeII GATC 1 cut(s) 312
NlaIII CATG 1 cut(s) 397
NlaIV GGNNCC 4 cut(s) 50, 321, 328, 385
NmuCI GTSAC 1 cut(s) 226
PceI AGGCCT 1 cut(s) 219
PfeI GAWTC 1 cut(s) 348
PkrI GCNGC 6 cut(s) 11, 58, 105, 108, 424, 472
PleI GAGTC 1 cut(s) 299
PpsI GAGTC 1 cut(s) 299
PpuMI RGGWCCY 1 cut(s) 384
Psp5II RGGWCCY 1 cut(s) 384
Psp6I CCWGG 1 cut(s) 322
PspEI GGTNACC 1 cut(s) 183
PspFI CCCAGC 1 cut(s) 371
PspGI CCWGG 1 cut(s) 322
PspN4I GGNNCC 4 cut(s) 50, 321, 328, 385
PspOMI GGGCCC 1 cut(s) 48
PspPI GGNCC 4 cut(s) 48, 49, 320, 384
PspPPI RGGWCCY 1 cut(s) 384
PsuI RGATCY 1 cut(s) 312
RsaI GTAC 1 cut(s) 328
RsaNI GTAC 1 cut(s) 327
SatI GCNGC 6 cut(s) 10, 57, 104, 107, 423, 471
Sau3AI GATC 1 cut(s) 312
Sau96I GGNCC 4 cut(s) 48, 49, 320, 384
SchI GAGTC 1 cut(s) 299
ScrFI CCNGG 1 cut(s) 324
SduI GDGCHC 1 cut(s) 52
SetI ASST 5 cut(s) 236, 269, 328, 389, 424
SexAI ACCWGGT 1 cut(s) 322
SfaNI GCATC 1 cut(s) 328
SfcI CTRYAG 1 cut(s) 13
SinI GGWCC 2 cut(s) 320, 384
SseBI AGGCCT 1 cut(s) 219
SsiI CCGC 3 cut(s) 106, 330, 471
StuI AGGCCT 1 cut(s) 219
StyD4I CCNGG 1 cut(s) 322
StyI CCWWGG 1 cut(s) 393
TaaI ACNGT 1 cut(s) 451
TaqI TCGA 1 cut(s) 255
TauI GCSGC 2 cut(s) 109, 473
TfiI GAWTC 1 cut(s) 348
TscAI CASTG 1 cut(s) 292
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 4 cut(s) 9, 56, 103, 422
Tsp45I GTSAC 1 cut(s) 226
TspGWI ACGGA 1 cut(s) 57
TspRI CASTG 1 cut(s) 292
VpaK11BI GGWCC 2 cut(s) 320, 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.