pycom07g00050

Vacuolar protein sorting-associated protein 62

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0009493
Physical Location & Seq
Forward (+)
60824 .. 61765
942 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g00050.1

Sequence Viewer

Length: 690 bp
ATGATTTTCCTCCATGCATTCTACCCTTTCAATGGCCCCGCCAGGGCCAAAGTTGAGTTTCTGAATCTCTCCCTAGGGAAAATTGGTGAACATATTGGTGACTGGGAGCACGTGACCCTACGAATCAGCAATTTTACTAAGGAACTCTGGAAGGTCTACTTCTCGCAGCACAACGGGTGCATATGGGTGGACGCTTCTGAGCTTGAGTTTCAGAATGGTGGTGGGAACAAAGCCGTGACCTATGCATCTTTGCACGACCATGCTTTTTATGCCAAGCCAAGACTTGTATTGCAAGGCACTGGAGGAATAGGAATTAGGAATGATGCTGCCAAGAGTAAGATGGTATTGGACACTGGACAAAGCCAGCCTATAATCATTGCAGTTGACTATTTGGGCTCCGTTATATCCGAACCGCCATGGCTCAACTACTATTGGGAATGGGATCCAAAACTTAGTTATGACATTACTGATGAACTAAAAAAGGTGGAGAAACTTTTGCCTGGAAAGCTCAAATCTGCATTTGAGAAATTCGTAAAGAGTCTACCAAATGAAGTGTTAGGGGAAGAAGGGCCGACTGGTCCCAAGATGAAGAACAACTGGACTAGTGATGAAAAAATGAGACTTTATTGTAGGAGGAAGGGAGAAATCTTAATTAAAACTTGGAGTAGTGGAGAAAGGAGACTCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps62

Protein Analysis

230

Amino Acids

26.14

Weight (kDa)

8.68

Isoelectric Point (pI)

31.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps62 PF06101 3 - 204 1.9e-91 Vacuolar protein sorting-associated protein 62
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 156, 541
AciI CCGC 2 cut(s) 39, 413
AclWI GGATC 2 cut(s) 437, 450
AcsI RAATTY 1 cut(s) 527
AcvI CACGTG 1 cut(s) 112
AfiI CCNNNNNNNGG 2 cut(s) 32, 43
AgsI TTSAA 1 cut(s) 31
AhlI ACTAGT 1 cut(s) 602
AjnI CCWGG 2 cut(s) 41, 499
AluBI AGCT 2 cut(s) 202, 508
AluI AGCT 2 cut(s) 202, 508
Alw21I GWGCWC 1 cut(s) 111
Alw26I GTCTC 2 cut(s) 613, 673
AlwI GGATC 2 cut(s) 437, 450
AoxI GGCC 3 cut(s) 34, 45, 569
ApeKI GCWGC 2 cut(s) 166, 326
ApoI RAATTY 1 cut(s) 527
AspA2I CCTAGG 1 cut(s) 73
AspS9I GGNCC 4 cut(s) 35, 45, 569, 578
AsuHPI GGTGA 2 cut(s) 98, 110
AvaII GGWCC 1 cut(s) 578
AvrII CCTAGG 1 cut(s) 73
BamHI GGATCC 1 cut(s) 442
BanII GRGCYC 1 cut(s) 398
BbrPI CACGTG 1 cut(s) 112
Bbv12I GWGCWC 1 cut(s) 111
BbvI GCAGC 2 cut(s) 178, 313
BccI CCATC 1 cut(s) 334
BceAI ACGGC 1 cut(s) 218
BciT130I CCWGG 2 cut(s) 43, 501
BcoDI GTCTC 2 cut(s) 613, 673
BcuI ACTAGT 1 cut(s) 602
BfaI CTAG 2 cut(s) 74, 603
BisI GCNGC 2 cut(s) 167, 327
BlnI CCTAGG 1 cut(s) 73
BlsI GCNGC 2 cut(s) 168, 328
Bme1390I CCNGG 2 cut(s) 43, 501
Bme18I GGWCC 1 cut(s) 578
BmgT120I GGNCC 4 cut(s) 35, 45, 569, 578
BmiI GGNNCC 4 cut(s) 37, 397, 444, 580
BmrFI CCNGG 2 cut(s) 43, 501
BmrI ACTGGG 1 cut(s) 112
BmsI GCATC 2 cut(s) 254, 313
BmuI ACTGGG 1 cut(s) 112
BpmI CTGGAG 1 cut(s) 321
BpuEI CTTGAG 1 cut(s) 224
BsaAI YACGTR 1 cut(s) 112
BsaJI CCNNGG 3 cut(s) 42, 73, 416
Bsc4I CCNNNNNNNGG 2 cut(s) 32, 43
Bse1I ACTGG 5 cut(s) 107, 304, 358, 580, 602
Bse3DI GCAATG 1 cut(s) 375
BseBI CCWGG 2 cut(s) 43, 501
BseDI CCNNGG 3 cut(s) 42, 73, 416
BseLI CCNNNNNNNGG 2 cut(s) 32, 43
BseMI GCAATG 1 cut(s) 375
BseMII CTCAG 1 cut(s) 189
BseNI ACTGG 5 cut(s) 107, 304, 358, 580, 602
BseXI GCAGC 2 cut(s) 178, 313
BshFI GGCC 3 cut(s) 36, 47, 571
BsiHKAI GWGCWC 1 cut(s) 111
BslFI GGGAC 1 cut(s) 564
BslI CCNNNNNNNGG 2 cut(s) 32, 43
BsmAI GTCTC 2 cut(s) 613, 673
BsmFI GGGAC 1 cut(s) 564
BsmI GAATGC 1 cut(s) 17
BsnI GGCC 3 cut(s) 36, 47, 571
Bsp1286I GDGCHC 2 cut(s) 111, 398
Bsp143I GATC 1 cut(s) 442
Bsp19I CCATGG 1 cut(s) 416
BspACI CCGC 2 cut(s) 39, 413
BspANI GGCC 3 cut(s) 36, 47, 571
BspCNI CTCAG 1 cut(s) 190
BspLI GGNNCC 4 cut(s) 37, 397, 444, 580
BspPI GGATC 2 cut(s) 437, 450
BsrDI GCAATG 1 cut(s) 375
BsrI ACTGG 5 cut(s) 107, 304, 358, 580, 602
BssECI CCNNGG 3 cut(s) 42, 73, 416
BssMI GATC 1 cut(s) 442
BssT1I CCWWGG 2 cut(s) 73, 416
Bst2UI CCWGG 2 cut(s) 43, 501
BstBAI YACGTR 1 cut(s) 112
BstC8I GCNNGC 1 cut(s) 365
BstDEI CTNAG 3 cut(s) 138, 198, 452
BstDSI CCRYGG 1 cut(s) 416
BstKTI GATC 1 cut(s) 445
BstMAI GTCTC 2 cut(s) 613, 673
BstMBI GATC 1 cut(s) 442
BstMWI GCNNNNNNNGC 2 cut(s) 269, 505
BstNI CCWGG 2 cut(s) 43, 501
BstSCI CCNGG 2 cut(s) 41, 499
BstV1I GCAGC 2 cut(s) 178, 313
BstX2I RGATCY 1 cut(s) 442
BstYI RGATCY 1 cut(s) 442
BsuRI GGCC 3 cut(s) 36, 47, 571
BtgI CCRYGG 1 cut(s) 416
BtsIMutI CAGTG 2 cut(s) 297, 351
Cac8I GCNNGC 1 cut(s) 365
Cfr13I GGNCC 4 cut(s) 35, 45, 569, 578
CseI GACGC 1 cut(s) 200
CviAII CATG 3 cut(s) 14, 260, 417
DdeI CTNAG 3 cut(s) 138, 198, 452
DpnI GATC 1 cut(s) 444
DpnII GATC 1 cut(s) 442
Eco130I CCWWGG 2 cut(s) 73, 416
Eco24I GRGCYC 1 cut(s) 398
Eco47I GGWCC 1 cut(s) 578
Eco72I CACGTG 1 cut(s) 112
EcoRII CCWGG 2 cut(s) 41, 499
EcoT14I CCWWGG 2 cut(s) 73, 416
EcoT22I ATGCAT 2 cut(s) 19, 247
EcoT38I GRGCYC 1 cut(s) 398
ErhI CCWWGG 2 cut(s) 73, 416
FaeI CATG 3 cut(s) 17, 263, 420
FalI AAGNNNNNCTT 2 cut(s) 143, 175
FaqI GGGAC 1 cut(s) 564
FatI CATG 3 cut(s) 13, 259, 416
FauI CCCGC 1 cut(s) 46
FauNDI CATATG 1 cut(s) 182
FblI GTMKAC 2 cut(s) 156, 541
Fnu4HI GCNGC 2 cut(s) 167, 327
FriOI GRGCYC 1 cut(s) 398
Fsp4HI GCNGC 2 cut(s) 167, 327
FspBI CTAG 2 cut(s) 74, 603
GluI GCNGC 2 cut(s) 167, 327
GsuI CTGGAG 1 cut(s) 321
HaeIII GGCC 3 cut(s) 36, 47, 571
HgaI GACGC 1 cut(s) 200
Hin1II CATG 3 cut(s) 17, 263, 420
HincII GTYRAC 1 cut(s) 385
HindII GTYRAC 1 cut(s) 385
HinfI GANTC 4 cut(s) 64, 123, 538, 681
HphI GGTGA 2 cut(s) 98, 110
Hpy166II GTNNAC 5 cut(s) 89, 157, 190, 385, 542
Hpy188I TCNGA 4 cut(s) 63, 199, 213, 409
Hpy188III TCNNGA 1 cut(s) 148
Hpy8I GTNNAC 5 cut(s) 89, 157, 190, 385, 542
HpyAV CCTTC 3 cut(s) 145, 560, 631
HpyCH4IV ACGT 1 cut(s) 111
HpyCH4V TGCA 7 cut(s) 17, 180, 245, 253, 292, 380, 518
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 505
HpyF3I CTNAG 3 cut(s) 138, 198, 452
HpySE526I ACGT 1 cut(s) 111
Hsp92II CATG 3 cut(s) 17, 263, 420
Kzo9I GATC 1 cut(s) 442
LmnI GCTCC 2 cut(s) 106, 401
Lsp1109I GCAGC 2 cut(s) 178, 313
LweI GCATC 2 cut(s) 254, 313
MaeI CTAG 2 cut(s) 74, 603
MaeII ACGT 1 cut(s) 111
MaeIII GTNAC 3 cut(s) 98, 112, 235
MalI GATC 1 cut(s) 444
MboI GATC 1 cut(s) 442
MboII GAAGA 2 cut(s) 575, 601
MflI RGATCY 1 cut(s) 442
MhlI GDGCHC 2 cut(s) 111, 398
MluCI AATT 5 cut(s) 81, 130, 312, 527, 651
MlyI GAGTC 2 cut(s) 547, 675
MnlI CCTC 3 cut(s) 20, 296, 627
Mph1103I ATGCAT 2 cut(s) 19, 247
MseI TTAA 3 cut(s) 650, 654, 688
MslI CAYNNNNRTG 3 cut(s) 96, 185, 258
MspR9I CCNGG 2 cut(s) 43, 501
Mva1269I GAATGC 1 cut(s) 17
MvaI CCWGG 2 cut(s) 43, 501
MwoI GCNNNNNNNGC 2 cut(s) 269, 505
NcoI CCATGG 1 cut(s) 416
NdeI CATATG 1 cut(s) 182
NdeII GATC 1 cut(s) 442
NlaIII CATG 3 cut(s) 17, 263, 420
NlaIV GGNNCC 4 cut(s) 37, 397, 444, 580
NmuCI GTSAC 3 cut(s) 98, 112, 235
NsiI ATGCAT 2 cut(s) 19, 247
PacI TTAATTAA 1 cut(s) 654
PctI GAATGC 1 cut(s) 17
PfeI GAWTC 2 cut(s) 64, 123
PkrI GCNGC 2 cut(s) 168, 328
PleI GAGTC 2 cut(s) 546, 675
PmaCI CACGTG 1 cut(s) 112
PmlI CACGTG 1 cut(s) 112
PpsI GAGTC 2 cut(s) 546, 675
Ppu21I YACGTR 1 cut(s) 112
Psp6I CCWGG 2 cut(s) 41, 499
PspCI CACGTG 1 cut(s) 112
PspGI CCWGG 2 cut(s) 41, 499
PspN4I GGNNCC 4 cut(s) 37, 397, 444, 580
PspPI GGNCC 4 cut(s) 35, 45, 569, 578
PsuI RGATCY 1 cut(s) 442
RseI CAYNNNNRTG 3 cut(s) 96, 185, 258
SaqAI TTAA 3 cut(s) 650, 654, 688
SatI GCNGC 2 cut(s) 167, 327
Sau3AI GATC 1 cut(s) 442
Sau96I GGNCC 4 cut(s) 35, 45, 569, 578
SchI GAGTC 2 cut(s) 547, 675
ScrFI CCNGG 2 cut(s) 43, 501
SduI GDGCHC 2 cut(s) 111, 398
SetI ASST 6 cut(s) 114, 156, 204, 242, 486, 510
SfaNI GCATC 2 cut(s) 254, 313
SinI GGWCC 1 cut(s) 578
SmiMI CAYNNNNRTG 3 cut(s) 96, 185, 258
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
SpeI ACTAGT 1 cut(s) 602
Sse9I AATT 5 cut(s) 81, 130, 312, 527, 651
SsiI CCGC 2 cut(s) 39, 413
SspMI CTAG 2 cut(s) 74, 603
StyD4I CCNGG 2 cut(s) 41, 499
StyI CCWWGG 2 cut(s) 73, 416
TaiI ACGT 1 cut(s) 114
TasI AATT 5 cut(s) 81, 130, 312, 527, 651
TfiI GAWTC 2 cut(s) 64, 123
Tru1I TTAA 3 cut(s) 650, 654, 688
Tru9I TTAA 3 cut(s) 650, 654, 688
TscAI CASTG 2 cut(s) 304, 358
TseFI GTSAC 3 cut(s) 98, 112, 235
TseI GCWGC 2 cut(s) 166, 326
Tsp45I GTSAC 3 cut(s) 98, 112, 235
TspDTI ATGAA 4 cut(s) 486, 564, 602, 624
TspGWI ACGGA 1 cut(s) 388
TspRI CASTG 2 cut(s) 304, 358
VpaK11BI GGWCC 1 cut(s) 578
XapI RAATTY 1 cut(s) 527
XcmI CCANNNNNNNNNTGG 1 cut(s) 337
XmaJI CCTAGG 1 cut(s) 73
XmiI GTMKAC 2 cut(s) 156, 541
XspI CTAG 2 cut(s) 74, 603
Zsp2I ATGCAT 2 cut(s) 19, 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.