MD07G1000600.v1.1

Vacuolar protein sorting-associated protein 62

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009493
Physical Location & Seq
Reverse (-)
117700 .. 117975
276 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1000600.v1.1.491

Sequence Viewer

Length: 231 bp
ATGATGGGCTCAACATTTACTGACATCACTATATGGGTGTTCTACCCTTTCAATGGCCCCGCCAGGGCCAATGAGTTTCTGAATATCTCCCTAGGGAAAATTGGTGAACATGTTGGCAACTGGGAGCACGTGACCCTACGAGTCAGCAATTTTATTGGGGAACTCTGGAAGGTCTACTTCTCGCAGCACAGCGGGGGCATATGGGTGGAAGCTTCCGAGCTTGAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps62

Protein Analysis

77

Amino Acids

8.71

Weight (kDa)

5.11

Isoelectric Point (pI)

19.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps62 PF06101 1 - 76 3.6e-40 Vacuolar protein sorting-associated protein 62
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 174
AciI CCGC 2 cut(s) 60, 192
AcvI CACGTG 1 cut(s) 130
AfiI CCNNNNNNNGG 2 cut(s) 53, 64
AflIII ACRYGT 1 cut(s) 109
AgsI TTSAA 1 cut(s) 52
AjnI CCWGG 1 cut(s) 62
AluBI AGCT 2 cut(s) 212, 220
AluI AGCT 2 cut(s) 212, 220
Alw21I GWGCWC 1 cut(s) 129
AoxI GGCC 2 cut(s) 55, 66
ApeKI GCWGC 1 cut(s) 184
AspA2I CCTAGG 1 cut(s) 91
AspS9I GGNCC 2 cut(s) 56, 66
AsuHPI GGTGA 1 cut(s) 116
AvrII CCTAGG 1 cut(s) 91
BanII GRGCYC 1 cut(s) 11
BbrPI CACGTG 1 cut(s) 130
Bbv12I GWGCWC 1 cut(s) 129
BbvI GCAGC 1 cut(s) 196
BciT130I CCWGG 1 cut(s) 64
BfaI CTAG 1 cut(s) 92
BisI GCNGC 1 cut(s) 185
BlnI CCTAGG 1 cut(s) 91
BlsI GCNGC 1 cut(s) 186
Bme1390I CCNGG 1 cut(s) 64
BmgT120I GGNCC 2 cut(s) 56, 66
BmiI GGNNCC 1 cut(s) 58
BmrFI CCNGG 1 cut(s) 64
BmrI ACTGGG 1 cut(s) 130
BmuI ACTGGG 1 cut(s) 130
BsaAI YACGTR 1 cut(s) 130
BsaJI CCNNGG 2 cut(s) 63, 91
Bsc4I CCNNNNNNNGG 2 cut(s) 53, 64
Bse1I ACTGG 1 cut(s) 125
BseBI CCWGG 1 cut(s) 64
BseDI CCNNGG 2 cut(s) 63, 91
BseLI CCNNNNNNNGG 2 cut(s) 53, 64
BseNI ACTGG 1 cut(s) 125
BseXI GCAGC 1 cut(s) 196
BshFI GGCC 2 cut(s) 57, 68
BsiHKAI GWGCWC 1 cut(s) 129
BslI CCNNNNNNNGG 2 cut(s) 53, 64
BsnI GGCC 2 cut(s) 57, 68
Bsp1286I GDGCHC 2 cut(s) 11, 129
BspACI CCGC 2 cut(s) 60, 192
BspANI GGCC 2 cut(s) 57, 68
BspLI GGNNCC 1 cut(s) 58
BsrI ACTGG 1 cut(s) 125
BssECI CCNNGG 2 cut(s) 63, 91
BssT1I CCWWGG 1 cut(s) 91
Bst2UI CCWGG 1 cut(s) 64
BstBAI YACGTR 1 cut(s) 130
BstNI CCWGG 1 cut(s) 64
BstNSI RCATGY 1 cut(s) 113
BstSCI CCNGG 1 cut(s) 62
BstV1I GCAGC 1 cut(s) 196
BsuRI GGCC 2 cut(s) 57, 68
Cfr13I GGNCC 2 cut(s) 56, 66
CviAII CATG 1 cut(s) 110
CviJI RGCY 5 cut(s) 9, 57, 68, 212, 220
CviKI_1 RGCY 5 cut(s) 9, 57, 68, 212, 220
Eco130I CCWWGG 1 cut(s) 91
Eco24I GRGCYC 1 cut(s) 11
Eco72I CACGTG 1 cut(s) 130
EcoRII CCWGG 1 cut(s) 62
EcoT14I CCWWGG 1 cut(s) 91
EcoT38I GRGCYC 1 cut(s) 11
ErhI CCWWGG 1 cut(s) 91
FaeI CATG 1 cut(s) 113
FaiI YATR 5 cut(s) 32, 34, 111, 200, 202
FalI AAGNNNNNCTT 2 cut(s) 161, 193
FatI CATG 1 cut(s) 109
FauI CCCGC 2 cut(s) 67, 185
FauNDI CATATG 1 cut(s) 200
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 1 cut(s) 185
FriOI GRGCYC 1 cut(s) 11
Fsp4HI GCNGC 1 cut(s) 185
FspBI CTAG 1 cut(s) 92
GluI GCNGC 1 cut(s) 185
HaeIII GGCC 2 cut(s) 57, 68
Hin1II CATG 1 cut(s) 113
HindIII AAGCTT 1 cut(s) 210
HinfI GANTC 1 cut(s) 141
HphI GGTGA 1 cut(s) 116
Hpy166II GTNNAC 2 cut(s) 107, 175
Hpy188I TCNGA 2 cut(s) 81, 217
Hpy188III TCNNGA 1 cut(s) 166
Hpy8I GTNNAC 2 cut(s) 107, 175
HpyAV CCTTC 1 cut(s) 163
HpyCH4IV ACGT 1 cut(s) 129
HpySE526I ACGT 1 cut(s) 129
Hsp92II CATG 1 cut(s) 113
LmnI GCTCC 1 cut(s) 124
LpnPI CCDG 4 cut(s) 49, 76, 106, 151
Lsp1109I GCAGC 1 cut(s) 196
MaeI CTAG 1 cut(s) 92
MaeII ACGT 1 cut(s) 129
MaeIII GTNAC 1 cut(s) 130
MhlI GDGCHC 2 cut(s) 11, 129
MluCI AATT 2 cut(s) 99, 148
MlyI GAGTC 1 cut(s) 150
MslI CAYNNNNRTG 1 cut(s) 203
MspA1I CMGCKG 1 cut(s) 192
MspR9I CCNGG 1 cut(s) 64
MvaI CCWGG 1 cut(s) 64
NdeI CATATG 1 cut(s) 200
NlaIII CATG 1 cut(s) 113
NlaIV GGNNCC 1 cut(s) 58
NmuCI GTSAC 1 cut(s) 130
NspI RCATGY 1 cut(s) 113
PciI ACATGT 1 cut(s) 109
PkrI GCNGC 1 cut(s) 186
PleI GAGTC 1 cut(s) 149
PmaCI CACGTG 1 cut(s) 130
PmlI CACGTG 1 cut(s) 130
PpsI GAGTC 1 cut(s) 149
Ppu21I YACGTR 1 cut(s) 130
PscI ACATGT 1 cut(s) 109
Psp6I CCWGG 1 cut(s) 62
PspCI CACGTG 1 cut(s) 130
PspGI CCWGG 1 cut(s) 62
PspN4I GGNNCC 1 cut(s) 58
PspPI GGNCC 2 cut(s) 56, 66
RseI CAYNNNNRTG 1 cut(s) 203
SatI GCNGC 1 cut(s) 185
Sau96I GGNCC 2 cut(s) 56, 66
SchI GAGTC 1 cut(s) 150
ScrFI CCNGG 1 cut(s) 64
SduI GDGCHC 2 cut(s) 11, 129
SetI ASST 4 cut(s) 132, 174, 214, 222
SmiMI CAYNNNNRTG 1 cut(s) 203
SmlI CTYRAG 1 cut(s) 221
SmoI CTYRAG 1 cut(s) 221
Sse9I AATT 2 cut(s) 99, 148
SsiI CCGC 2 cut(s) 60, 192
SspMI CTAG 1 cut(s) 92
StyD4I CCNGG 1 cut(s) 62
StyI CCWWGG 1 cut(s) 91
TaiI ACGT 1 cut(s) 132
TasI AATT 2 cut(s) 99, 148
TseFI GTSAC 1 cut(s) 130
TseI GCWGC 1 cut(s) 184
Tsp45I GTSAC 1 cut(s) 130
XceI RCATGY 1 cut(s) 113
XmaJI CCTAGG 1 cut(s) 91
XmiI GTMKAC 1 cut(s) 174
XspI CTAG 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.