Rroxscaffold_4G00329070

Vacuolar protein sorting-associated protein 62

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0009493
Physical Location & Seq
Reverse (-)
62278923 .. 62281476
2554 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00329070.1

Sequence Viewer

Length: 1665 bp
ATGGGAAATTGCCTTTCCCTCTCATGTTCAACCAAGAACATCAAGGCGAATCCGATCGAAACCACATTCAAGCTTCCCTCTCCATTACCCAGTTGGCCATCTGGTAATGGGTTCGCAAATGGAATCATAAATCTGGGAGGATTAAAAGTATGCCAAATTTCATCTTTCAACAAAGTATGGGCAACCCATGAGGGAGGACCAGGCAACCTTGGCGCAACCTTCTTCGAACCTTCACCCCTACCCCAAGGATTCTATATGCTCGGTTGCTACAGCCAACCCAACAACAAACCCCTTTTCGGATGGGCCCTTGCTGCCAAAGCCGACGATCACTCCTCCTCTCCATGGCCGGCTTTACAGCAACCAGTTGATTACACACTGGTATGGAGCAGCGAGTCCGTGAAGATGAAGAAAGATGGTGATGGATACCTCTGGTTACCCACTGCCCCGGAGCGTTATGCAGCCATAGGCCATGTTGTCACTAGCTCTCCTGACAAGCCGTCCCTTGATAAAATCAGGTGCGTTCGATCTGACCTTACTGACCAATGTGAAGCTGACTCGTGGATCTGGGGCCCCCCTGGTGGCACCATTGATGGGTTCAATGTATATAGTCTCACACCCAGCAATAGGGGCATCCAAGCTATGGGTGTCCCCGTTGGCACATTCCTAGCCAGTAATGGTAAAGCAAATAATATAGCTTGTTTGAAAAATAATAACACCCAACCACACACTTGTATGCCAAACCAAAGCCAAATTGATGCAATATTTCAAGCCTACGCTCCATTCATATACTTCCATCCCGACGAAGCTTACCTCCCTTCTTCGGTGGGTTGGTTTTTCACAAATGGATCACTTCTGTATAGCAAGGAACATGTAGAAAATCCCGTTCCAATTGAACCCACGGGCTCTAATCTTCCACAAGGCGGCCCAAATGATGGCGCCTATTGGTTGGACCTACCTGTCGATCAAGGCGCCGAAGAACGAGTGAAAAAGGGAGATCTTGCTGACGCCGAGGCCTACTTACACATAAAACCCATGTTAGGCTCAACATTCACTGACATAGCTATTTGGGTCTTCTATCCTTTCAATGGCCCCGCAAAGGCCAAAGTCGAGTTCTTCAATGTTTCGTTGGGCAAGATAGGTGAACATGTTGGTGATTGGGAGCATGTCACATTAAGAGTCAGCAATTTCACTGGCCAACTCTGGAGTGTCTACTTCTCGGAACACAGCGGGGGAATATGGGTGGACGCTTCTGGGCTTGAATTTCAAAATGGCAACAAACCGGTGACATATGCATCTTTGCATGGACATGCCTTCTATTCCAAACCAGGACTTGTTTTGCAAGGGAATGGAAGCATAGGAATCAGGAATGATACTGCAAAGAGTAAAATGGTAATGGACTCTGGACTGAGGCCTTCAATTATTGCCGCCGAGTATCTGGGCGCGGCTATCATTGAACCACCATGGCTTAACTACAGCAGGAAATGGGGTCCGAAAATTAGTTATCAGATTTCGGATGAAATAAAAAAGGTGGAGAAGCTCTTGCCTGGAAAGCTAAAGACTGTTTTTGACAAGTTTATCAAAAGTCTACCAGATGAAGTATTGGGAGAAGAAGGGCCCACAGGTCCCAAGATGAAGAATAACTGGAATGGAGATGAAGTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps62

Protein Analysis

554

Amino Acids

60.46

Weight (kDa)

5.87

Isoelectric Point (pI)

39.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps62 PF06101 20 - 552 2.9e-253 Vacuolar protein sorting-associated protein 62
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 956
AccB1I GGYRCC 3 cut(s) 581, 935, 968
AccB7I CCANNNNNTGG 2 cut(s) 640, 932
AccI GTMKAC 2 cut(s) 1209, 1585
AccII CGCG 1 cut(s) 1442
AciI CCGC 5 cut(s) 921, 1092, 1227, 1425, 1442
AclWI GGATC 2 cut(s) 569, 853
AcoI YGGCCR 3 cut(s) 95, 344, 1192
AcsI RAATTY 2 cut(s) 156, 1259
AcyI GRCGYC 3 cut(s) 936, 969, 1005
AflIII ACRYGT 2 cut(s) 868, 1144
AgeI ACCGGT 1 cut(s) 1279
AhdI GACNNNNNGTC 1 cut(s) 496
AjnI CCWGG 4 cut(s) 199, 574, 1324, 1543
AjuI GAANNNNNNNTTGG 2 cut(s) 1094, 1126
AluBI AGCT 9 cut(s) 73, 483, 551, 638, 695, 806, 1061, 1537, 1552
AluI AGCT 9 cut(s) 73, 483, 551, 638, 695, 806, 1061, 1537, 1552
Alw26I GTCTC 1 cut(s) 614
AlwI GGATC 2 cut(s) 569, 853
ApaI GGGCCC 3 cut(s) 307, 572, 1617
ApeKI GCWGC 3 cut(s) 311, 387, 458
ApoI RAATTY 2 cut(s) 156, 1259
AsiGI ACCGGT 1 cut(s) 1279
AspLEI GCGC 4 cut(s) 215, 938, 971, 1442
AsuC2I CCSGG 1 cut(s) 446
AsuHPI GGTGA 5 cut(s) 225, 428, 1151, 1163, 1294
AsuII TTCGAA 1 cut(s) 225
AvaII GGWCC 4 cut(s) 197, 949, 1487, 1622
BaeGI GKGCMC 3 cut(s) 307, 572, 1617
BalI TGGCCA 2 cut(s) 97, 1194
BanI GGYRCC 3 cut(s) 581, 935, 968
BanII GRGCYC 4 cut(s) 307, 572, 905, 1617
BauI CACGAG 1 cut(s) 556
BbsI GAAGAC 1 cut(s) 1063
BbvI GCAGC 3 cut(s) 298, 399, 470
BccI CCATC 7 cut(s) 106, 294, 407, 413, 584, 801, 926
BceAI ACGGC 1 cut(s) 481
BciT130I CCWGG 4 cut(s) 201, 576, 1326, 1545
BciVI GTATCC 1 cut(s) 416
BcnI CCSGG 1 cut(s) 446
BcoDI GTCTC 1 cut(s) 614
BfaI CTAG 2 cut(s) 480, 665
BfmI CTRYAG 2 cut(s) 268, 1471
BfoI RGCGCY 2 cut(s) 939, 972
BfuI GTATCC 1 cut(s) 416
BglII AGATCT 1 cut(s) 994
BisI GCNGC 6 cut(s) 312, 388, 459, 922, 1425, 1443
BlsI GCNGC 6 cut(s) 313, 389, 460, 923, 1426, 1444
Bme1390I CCNGG 5 cut(s) 201, 446, 576, 1326, 1545
Bme18I GGWCC 4 cut(s) 197, 949, 1487, 1622
BmeRI GACNNNNNGTC 1 cut(s) 496
BmrFI CCNGG 5 cut(s) 201, 446, 576, 1326, 1545
BmrI ACTGGG 1 cut(s) 84
BmsI GCATC 3 cut(s) 639, 745, 1301
BmuI ACTGGG 1 cut(s) 84
BpiI GAAGAC 1 cut(s) 1063
BpmI CTGGAG 1 cut(s) 1222
Bpu14I TTCGAA 1 cut(s) 225
BpuMI CCSGG 1 cut(s) 446
BsaHI GRCGYC 3 cut(s) 936, 969, 1005
BsaJI CCNNGG 8 cut(s) 208, 244, 341, 444, 574, 897, 1008, 1460
BsaWI WCCGGW 1 cut(s) 1279
BsaXI ACNNNNNCTCC 5 cut(s) 314, 344, 469, 499, 1641
Bse118I RCCGGY 2 cut(s) 346, 1279
Bse1I ACTGG 6 cut(s) 90, 362, 381, 669, 1195, 1646
BseBI CCWGG 4 cut(s) 201, 576, 1326, 1545
BseDI CCNNGG 8 cut(s) 208, 244, 341, 444, 574, 897, 1008, 1460
BseGI GGATG 4 cut(s) 305, 630, 793, 1519
BseMII CTCAG 1 cut(s) 1397
BseNI ACTGG 6 cut(s) 90, 362, 381, 669, 1195, 1646
BseRI GAGGAG 2 cut(s) 322, 325
BseSI GKGCMC 3 cut(s) 307, 572, 1617
BseXI GCAGC 3 cut(s) 298, 399, 470
BseYI CCCAGC 1 cut(s) 617
Bsh1236I CGCG 1 cut(s) 1442
Bsh1285I CGRYCG 1 cut(s) 57
BshNI GGYRCC 3 cut(s) 581, 935, 968
BshTI ACCGGT 1 cut(s) 1279
BsiEI CGRYCG 1 cut(s) 57
BsiSI CCGG 3 cut(s) 347, 446, 1280
BslFI GGGAC 3 cut(s) 484, 632, 1608
BsmAI GTCTC 1 cut(s) 614
BsmFI GGGAC 3 cut(s) 484, 632, 1608
Bsp119I TTCGAA 1 cut(s) 225
Bsp120I GGGCCC 3 cut(s) 303, 568, 1613
Bsp1286I GDGCHC 4 cut(s) 307, 572, 905, 1617
Bsp143I GATC 7 cut(s) 54, 325, 524, 561, 845, 961, 994
Bsp19I CCATGG 2 cut(s) 341, 1460
BspACI CCGC 5 cut(s) 921, 1092, 1227, 1425, 1442
BspCNI CTCAG 1 cut(s) 1398
BspFNI CGCG 1 cut(s) 1442
BspPI GGATC 2 cut(s) 569, 853
BspT104I TTCGAA 1 cut(s) 225
BspT107I GGYRCC 3 cut(s) 581, 935, 968
BsrFI RCCGGY 2 cut(s) 346, 1279
BsrI ACTGG 6 cut(s) 90, 362, 381, 669, 1195, 1646
BssAI RCCGGY 2 cut(s) 346, 1279
BssECI CCNNGG 8 cut(s) 208, 244, 341, 444, 574, 897, 1008, 1460
BssMI GATC 7 cut(s) 54, 325, 524, 561, 845, 961, 994
BssNI GRCGYC 3 cut(s) 936, 969, 1005
BssSI CACGAG 1 cut(s) 556
BssT1I CCWWGG 4 cut(s) 208, 244, 341, 1460
Bst2BI CACGAG 1 cut(s) 556
Bst2UI CCWGG 4 cut(s) 201, 576, 1326, 1545
Bst4CI ACNGT 1 cut(s) 1561
BstACI GRCGYC 3 cut(s) 936, 969, 1005
BstBI TTCGAA 1 cut(s) 225
BstC8I GCNNGC 1 cut(s) 348
BstDEI CTNAG 1 cut(s) 1406
BstDSI CCRYGG 3 cut(s) 341, 897, 1460
BstEII GGTNACC 1 cut(s) 432
BstF5I GGATG 4 cut(s) 305, 630, 793, 1519
BstFNI CGCG 1 cut(s) 1442
BstH2I RGCGCY 2 cut(s) 939, 972
BstHHI GCGC 4 cut(s) 215, 938, 971, 1442
BstKTI GATC 7 cut(s) 57, 328, 527, 564, 848, 964, 997
BstMAI GTCTC 1 cut(s) 614
BstMBI GATC 7 cut(s) 54, 325, 524, 561, 845, 961, 994
BstMCI CGRYCG 1 cut(s) 57
BstMWI GCNNNNNNNGC 5 cut(s) 210, 311, 317, 627, 1549
BstNI CCWGG 4 cut(s) 201, 576, 1326, 1545
BstNSI RCATGY 4 cut(s) 872, 1148, 1166, 1310
BstPI GGTNACC 1 cut(s) 432
BstSCI CCNGG 5 cut(s) 199, 444, 574, 1324, 1543
BstSFI CTRYAG 2 cut(s) 268, 1471
BstSLI GKGCMC 3 cut(s) 307, 572, 1617
BstUI CGCG 1 cut(s) 1442
BstV1I GCAGC 3 cut(s) 298, 399, 470
BstV2I GAAGAC 1 cut(s) 1063
BstX2I RGATCY 2 cut(s) 561, 994
BstYI RGATCY 2 cut(s) 561, 994
BsuI GTATCC 1 cut(s) 416
BtgI CCRYGG 3 cut(s) 341, 897, 1460
BtsCI GGATG 4 cut(s) 305, 630, 793, 1519
BtsI GCAGTG 1 cut(s) 438
BtsIMutI CAGTG 4 cut(s) 374, 438, 1050, 1188
Cac8I GCNNGC 1 cut(s) 348
CfoI GCGC 4 cut(s) 215, 938, 971, 1442
Cfr10I RCCGGY 2 cut(s) 346, 1279
CseI GACGC 2 cut(s) 1013, 1253
CspAI ACCGGT 1 cut(s) 1279
DdeI CTNAG 1 cut(s) 1406
DinI GGCGCC 2 cut(s) 937, 970
DpnI GATC 7 cut(s) 56, 327, 526, 563, 847, 963, 996
DpnII GATC 7 cut(s) 54, 325, 524, 561, 845, 961, 994
DrdI GACNNNNNNGTC 1 cut(s) 956
DriI GACNNNNNGTC 1 cut(s) 496
DseDI GACNNNNNNGTC 1 cut(s) 956
EaeI YGGCCR 3 cut(s) 95, 344, 1192
Eam1105I GACNNNNNGTC 1 cut(s) 496
Eco130I CCWWGG 4 cut(s) 208, 244, 341, 1460
Eco147I AGGCCT 2 cut(s) 1013, 1411
Eco24I GRGCYC 4 cut(s) 307, 572, 905, 1617
Eco47I GGWCC 4 cut(s) 197, 949, 1487, 1622
Eco91I GGTNACC 1 cut(s) 432
EcoO109I RGGNCCY 5 cut(s) 304, 568, 569, 1613, 1622
EcoO65I GGTNACC 1 cut(s) 432
EcoRII CCWGG 4 cut(s) 199, 574, 1324, 1543
EcoT14I CCWWGG 4 cut(s) 208, 244, 341, 1460
EcoT22I ATGCAT 1 cut(s) 1294
EcoT38I GRGCYC 4 cut(s) 307, 572, 905, 1617
EgeI GGCGCC 2 cut(s) 937, 970
EheI GGCGCC 2 cut(s) 937, 970
ErhI CCWWGG 4 cut(s) 208, 244, 341, 1460
FaqI GGGAC 3 cut(s) 484, 632, 1608
FauI CCCGC 2 cut(s) 1099, 1220
FauNDI CATATG 1 cut(s) 1288
FblI GTMKAC 2 cut(s) 1209, 1585
Fnu4HI GCNGC 6 cut(s) 312, 388, 459, 922, 1425, 1443
FokI GGATG 4 cut(s) 312, 617, 780, 1526
FriOI GRGCYC 4 cut(s) 307, 572, 905, 1617
Fsp4HI GCNGC 6 cut(s) 312, 388, 459, 922, 1425, 1443
FspBI CTAG 2 cut(s) 480, 665
GlaI GCGC 4 cut(s) 214, 937, 970, 1441
GluI GCNGC 6 cut(s) 312, 388, 459, 922, 1425, 1443
GsaI CCCAGC 1 cut(s) 621
GsuI CTGGAG 1 cut(s) 1222
HaeII RGCGCY 2 cut(s) 939, 972
HapII CCGG 3 cut(s) 347, 446, 1280
HgaI GACGC 2 cut(s) 1013, 1253
HhaI GCGC 4 cut(s) 215, 938, 971, 1442
Hin1I GRCGYC 3 cut(s) 936, 969, 1005
Hin6I GCGC 4 cut(s) 213, 936, 969, 1440
HinP1I GCGC 4 cut(s) 213, 936, 969, 1440
HindIII AAGCTT 2 cut(s) 71, 804
HinfI GANTC 8 cut(s) 49, 123, 249, 392, 554, 1176, 1359, 1397
HpaII CCGG 3 cut(s) 347, 446, 1280
HphI GGTGA 5 cut(s) 225, 428, 1151, 1163, 1294
Hpy166II GTNNAC 4 cut(s) 1142, 1210, 1243, 1586
Hpy188I TCNGA 7 cut(s) 54, 299, 529, 1219, 1491, 1506, 1513
Hpy188III TCNNGA 5 cut(s) 488, 797, 1201, 1363, 1401
Hpy8I GTNNAC 4 cut(s) 1142, 1210, 1243, 1586
Hpy99I CGWCG 2 cut(s) 326, 803
HpyAV CCTTC 6 cut(s) 229, 240, 825, 1321, 1422, 1604
HpyCH4III ACNGT 1 cut(s) 1561
HpyCH4V TGCA 6 cut(s) 458, 758, 1292, 1300, 1339, 1376
HpyF10VI GCNNNNNNNGC 5 cut(s) 210, 311, 317, 627, 1549
HpyF3I CTNAG 1 cut(s) 1406
Hsp92I GRCGYC 3 cut(s) 936, 969, 1005
HspAI GCGC 4 cut(s) 213, 936, 969, 1440
KasI GGCGCC 2 cut(s) 935, 968
KroI GCCGGC 1 cut(s) 346
KroNI GCCGGC 1 cut(s) 348
Kzo9I GATC 7 cut(s) 54, 325, 524, 561, 845, 961, 994
LmnI GCTCC 4 cut(s) 384, 448, 781, 1159
Lsp1109I GCAGC 3 cut(s) 298, 399, 470
LweI GCATC 3 cut(s) 639, 745, 1301
MaeI CTAG 2 cut(s) 480, 665
MaeIII GTNAC 4 cut(s) 432, 475, 1165, 1282
MalI GATC 7 cut(s) 56, 327, 526, 563, 847, 963, 996
MboI GATC 7 cut(s) 54, 325, 524, 561, 845, 961, 994
MfeI CAATTG 1 cut(s) 888
MflI RGATCY 2 cut(s) 561, 994
MhlI GDGCHC 4 cut(s) 307, 572, 905, 1617
MlsI TGGCCA 2 cut(s) 97, 1194
MluCI AATT 8 cut(s) 7, 156, 750, 888, 1183, 1259, 1416, 1494
MluNI TGGCCA 2 cut(s) 97, 1194
Mly113I GGCGCC 2 cut(s) 936, 969
MlyI GAGTC 4 cut(s) 401, 548, 1185, 1391
MmeI TCCRAC 1 cut(s) 927
Mox20I TGGCCA 2 cut(s) 97, 1194
Mph1103I ATGCAT 1 cut(s) 1294
MroNI GCCGGC 1 cut(s) 346
MscI TGGCCA 2 cut(s) 97, 1194
MseI TTAA 3 cut(s) 143, 1172, 1467
MslI CAYNNNNRTG 4 cut(s) 379, 731, 1149, 1305
Msp20I TGGCCA 2 cut(s) 97, 1194
MspA1I CMGCKG 1 cut(s) 1227
MspI CCGG 3 cut(s) 347, 446, 1280
MspR9I CCNGG 5 cut(s) 201, 446, 576, 1326, 1545
MunI CAATTG 1 cut(s) 888
MvaI CCWGG 4 cut(s) 201, 576, 1326, 1545
MvnI CGCG 1 cut(s) 1442
MwoI GCNNNNNNNGC 5 cut(s) 210, 311, 317, 627, 1549
NaeI GCCGGC 1 cut(s) 348
NarI GGCGCC 2 cut(s) 936, 969
NciI CCSGG 1 cut(s) 446
NcoI CCATGG 2 cut(s) 341, 1460
NdeI CATATG 1 cut(s) 1288
NdeII GATC 7 cut(s) 54, 325, 524, 561, 845, 961, 994
NgoMIV GCCGGC 1 cut(s) 346
NmeAIII GCCGAG 2 cut(s) 1033, 1453
NmuCI GTSAC 3 cut(s) 475, 1165, 1282
NsiI ATGCAT 1 cut(s) 1294
NspI RCATGY 4 cut(s) 872, 1148, 1166, 1310
NspV TTCGAA 1 cut(s) 225
PceI AGGCCT 2 cut(s) 1013, 1411
PciI ACATGT 2 cut(s) 868, 1144
PdiI GCCGGC 1 cut(s) 348
PfeI GAWTC 4 cut(s) 49, 123, 249, 1359
PflMI CCANNNNNTGG 2 cut(s) 640, 932
PinAI ACCGGT 1 cut(s) 1279
PkrI GCNGC 6 cut(s) 313, 389, 460, 923, 1426, 1444
Ple19I CGATCG 1 cut(s) 57
PleI GAGTC 4 cut(s) 400, 548, 1184, 1391
PluTI GGCGCC 2 cut(s) 939, 972
PpsI GAGTC 4 cut(s) 400, 548, 1184, 1391
PpuMI RGGWCCY 1 cut(s) 1622
PscI ACATGT 2 cut(s) 868, 1144
Psp5II RGGWCCY 1 cut(s) 1622
Psp6I CCWGG 4 cut(s) 199, 574, 1324, 1543
PspEI GGTNACC 1 cut(s) 432
PspFI CCCAGC 1 cut(s) 617
PspGI CCWGG 4 cut(s) 199, 574, 1324, 1543
PspOMI GGGCCC 3 cut(s) 303, 568, 1613
PspPPI RGGWCCY 1 cut(s) 1622
PsuI RGATCY 2 cut(s) 561, 994
PvuI CGATCG 1 cut(s) 57
RseI CAYNNNNRTG 4 cut(s) 379, 731, 1149, 1305
SaqAI TTAA 3 cut(s) 143, 1172, 1467
SatI GCNGC 6 cut(s) 312, 388, 459, 922, 1425, 1443
Sau3AI GATC 7 cut(s) 54, 325, 524, 561, 845, 961, 994
SchI GAGTC 4 cut(s) 401, 548, 1185, 1391
ScrFI CCNGG 5 cut(s) 201, 446, 576, 1326, 1545
SduI GDGCHC 4 cut(s) 307, 572, 905, 1617
SfaNI GCATC 3 cut(s) 639, 745, 1301
SfcI CTRYAG 2 cut(s) 268, 1471
SfoI GGCGCC 2 cut(s) 937, 970
SfuI TTCGAA 1 cut(s) 225
SinI GGWCC 4 cut(s) 197, 949, 1487, 1622
SmiMI CAYNNNNRTG 4 cut(s) 379, 731, 1149, 1305
Sse9I AATT 8 cut(s) 7, 156, 750, 888, 1183, 1259, 1416, 1494
SseBI AGGCCT 2 cut(s) 1013, 1411
SsiI CCGC 5 cut(s) 921, 1092, 1227, 1425, 1442
SspDI GGCGCC 2 cut(s) 935, 968
SspI AATATT 1 cut(s) 762
SspMI CTAG 2 cut(s) 480, 665
StuI AGGCCT 2 cut(s) 1013, 1411
StyD4I CCNGG 5 cut(s) 199, 444, 574, 1324, 1543
StyI CCWWGG 4 cut(s) 208, 244, 341, 1460
TaaI ACNGT 1 cut(s) 1561
TaqI TCGA 5 cut(s) 57, 225, 523, 960, 1107
TasI AATT 8 cut(s) 7, 156, 750, 888, 1183, 1259, 1416, 1494
TauI GCSGC 3 cut(s) 924, 1427, 1445
TfiI GAWTC 4 cut(s) 49, 123, 249, 1359
Tru1I TTAA 3 cut(s) 143, 1172, 1467
Tru9I TTAA 3 cut(s) 143, 1172, 1467
TscAI CASTG 4 cut(s) 381, 445, 1057, 1195
TseFI GTSAC 3 cut(s) 475, 1165, 1282
TseI GCWGC 3 cut(s) 311, 387, 458
Tsp45I GTSAC 3 cut(s) 475, 1165, 1282
TspDTI ATGAA 6 cut(s) 150, 419, 772, 1530, 1608, 1646
TspGWI ACGGA 1 cut(s) 385
TspRI CASTG 4 cut(s) 381, 445, 1057, 1195
Van91I CCANNNNNTGG 2 cut(s) 640, 932
VpaK11BI GGWCC 4 cut(s) 197, 949, 1487, 1622
XapI RAATTY 2 cut(s) 156, 1259
XceI RCATGY 4 cut(s) 872, 1148, 1166, 1310
XcmI CCANNNNNNNNNTGG 1 cut(s) 90
XmiI GTMKAC 2 cut(s) 1209, 1585
XspI CTAG 2 cut(s) 480, 665
Zsp2I ATGCAT 1 cut(s) 1294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.