MD07G1000300.v1.1

Vacuolar protein sorting-associated protein 62

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009493
Physical Location & Seq
Forward (+)
56933 .. 57535
603 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1000300.v1.1.491

Sequence Viewer

Length: 603 bp
ATGAACATGATATATATGGATCATATTTTATTAGGTAATGGGTTCGCAAGTGGAATGATCGATCTGGGAGGATTACTAGTGTGTCAAATATCGTCTTTCAACAAAGTTTGGGCTACTCATGAGGGGGGACCAGGCAACCTTGGGGCAAGCTTCTTCGAACCTTCACCCCTACCCCAAGGATTCTACATGCTTGGCTGCTACAGCCAACCCAACAACAAACCCCTTTGCGGATGGGCCCTTGCTGCTAAACAGACAAAAGACAACGACGATGACCCTTCTTCGCCCTTGCTGCGGCAACCAGTTGATTTCACTCTCGTTTGGAGCAGTGAGTTCTTGAACATCAAGAAAGATGGCAATGGCTATGTCTGGTTACCCACCGCCTCAGATGGTTATAAAGCCATAGGCCTTGTTGTCACTAGCTCTCCGGAGAAGCCATCCCTCGATAAAATCAGGTGCGTTCGTTCCAGCCTCACTGACGAGTGCGAGGCCGACTCGTGGATCTGGGGACAACCGGTCCGCGCGGCAATGGATTCAATGTCTTTAGCCTCAGACCCAGCAACAGGGGGACCCAAGCCATGGGTGTCTCCGCTGGCACTTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps62

Protein Analysis

201

Amino Acids

21.62

Weight (kDa)

4.79

Isoelectric Point (pI)

54.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps62 PF06101 9 - 181 1.4e-61 Vacuolar protein sorting-associated protein 62
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 393
AccB7I CCANNNNNTGG 1 cut(s) 576
AccII CGCG 2 cut(s) 519, 521
AccIII TCCGGA 1 cut(s) 424
AciI CCGC 6 cut(s) 228, 292, 378, 517, 521, 587
AclWI GGATC 2 cut(s) 27, 506
AfiI CCNNNNNNNGG 5 cut(s) 227, 291, 495, 560, 576
AgeI ACCGGT 1 cut(s) 511
AgsI TTSAA 3 cut(s) 100, 337, 534
AhdI GACNNNNNGTC 1 cut(s) 512
AhlI ACTAGT 1 cut(s) 76
AjnI CCWGG 1 cut(s) 130
AluBI AGCT 2 cut(s) 150, 420
AluI AGCT 2 cut(s) 150, 420
Alw26I GTCTC 1 cut(s) 588
AlwI GGATC 2 cut(s) 27, 506
Aor13HI TCCGGA 1 cut(s) 424
AoxI GGCC 3 cut(s) 234, 403, 486
ApaI GGGCCC 1 cut(s) 238
ApeKI GCWGC 3 cut(s) 195, 242, 289
AsiGI ACCGGT 1 cut(s) 511
AspLEI GCGC 1 cut(s) 521
AspS9I GGNCC 5 cut(s) 128, 234, 235, 514, 566
AsuHPI GGTGA 1 cut(s) 156
AsuII TTCGAA 1 cut(s) 156
AvaII GGWCC 3 cut(s) 128, 514, 566
BaeGI GKGCMC 1 cut(s) 238
BanII GRGCYC 1 cut(s) 238
BauI CACGAG 1 cut(s) 493
BbvI GCAGC 3 cut(s) 182, 229, 276
BccI CCATC 4 cut(s) 225, 344, 380, 442
BciT130I CCWGG 1 cut(s) 132
BcoDI GTCTC 1 cut(s) 588
BcuI ACTAGT 1 cut(s) 76
BfaI CTAG 2 cut(s) 77, 417
BfmI CTRYAG 1 cut(s) 199
BisI GCNGC 5 cut(s) 196, 243, 290, 293, 522
BlsI GCNGC 5 cut(s) 197, 244, 291, 294, 523
Bme1390I CCNGG 1 cut(s) 132
Bme18I GGWCC 3 cut(s) 128, 514, 566
BmeRI GACNNNNNGTC 1 cut(s) 512
BmgT120I GGNCC 5 cut(s) 128, 234, 235, 514, 566
BmiI GGNNCC 4 cut(s) 129, 236, 567, 568
BmrFI CCNGG 1 cut(s) 132
BplI GAGNNNNNCTC 2 cut(s) 476, 508
Bpu14I TTCGAA 1 cut(s) 156
Bsa29I ATCGAT 1 cut(s) 60
BsaJI CCNNGG 3 cut(s) 139, 175, 575
BsaWI WCCGGW 2 cut(s) 424, 511
BsaXI ACNNNNNCTCC 2 cut(s) 406, 436
Bsc4I CCNNNNNNNGG 5 cut(s) 227, 291, 495, 560, 576
Bse118I RCCGGY 1 cut(s) 511
Bse1I ACTGG 1 cut(s) 299
Bse3DI GCAATG 2 cut(s) 361, 531
BseAI TCCGGA 1 cut(s) 424
BseBI CCWGG 1 cut(s) 132
BseCI ATCGAT 1 cut(s) 60
BseDI CCNNGG 3 cut(s) 139, 175, 575
BseGI GGATG 2 cut(s) 236, 434
BseLI CCNNNNNNNGG 5 cut(s) 227, 291, 495, 560, 576
BseMI GCAATG 2 cut(s) 361, 531
BseMII CTCAG 2 cut(s) 396, 561
BseNI ACTGG 1 cut(s) 299
BseSI GKGCMC 1 cut(s) 238
BseXI GCAGC 3 cut(s) 182, 229, 276
BseYI CCCAGC 1 cut(s) 553
Bsh1236I CGCG 2 cut(s) 519, 521
BshFI GGCC 3 cut(s) 236, 405, 488
BshTI ACCGGT 1 cut(s) 511
BshVI ATCGAT 1 cut(s) 60
BsiSI CCGG 2 cut(s) 425, 512
BslFI GGGAC 3 cut(s) 141, 519, 579
BslI CCNNNNNNNGG 5 cut(s) 227, 291, 495, 560, 576
BsmAI GTCTC 1 cut(s) 588
BsmFI GGGAC 3 cut(s) 141, 519, 579
BsnI GGCC 3 cut(s) 236, 405, 488
Bsp119I TTCGAA 1 cut(s) 156
Bsp120I GGGCCC 1 cut(s) 234
Bsp1286I GDGCHC 1 cut(s) 238
Bsp13I TCCGGA 1 cut(s) 424
Bsp143I GATC 4 cut(s) 19, 57, 61, 498
Bsp19I CCATGG 1 cut(s) 575
BspACI CCGC 6 cut(s) 228, 292, 378, 517, 521, 587
BspANI GGCC 3 cut(s) 236, 405, 488
BspCNI CTCAG 2 cut(s) 395, 560
BspDI ATCGAT 1 cut(s) 60
BspEI TCCGGA 1 cut(s) 424
BspFNI CGCG 2 cut(s) 519, 521
BspHI TCATGA 1 cut(s) 118
BspLI GGNNCC 4 cut(s) 129, 236, 567, 568
BspPI GGATC 2 cut(s) 27, 506
BspT104I TTCGAA 1 cut(s) 156
BsrDI GCAATG 2 cut(s) 361, 531
BsrFI RCCGGY 1 cut(s) 511
BsrI ACTGG 1 cut(s) 299
BssAI RCCGGY 1 cut(s) 511
BssECI CCNNGG 3 cut(s) 139, 175, 575
BssMI GATC 4 cut(s) 19, 57, 61, 498
BssSI CACGAG 1 cut(s) 493
BssT1I CCWWGG 3 cut(s) 139, 175, 575
Bst2BI CACGAG 1 cut(s) 493
Bst2UI CCWGG 1 cut(s) 132
BstBI TTCGAA 1 cut(s) 156
BstC8I GCNNGC 2 cut(s) 148, 591
BstDEI CTNAG 2 cut(s) 382, 547
BstDSI CCRYGG 1 cut(s) 575
BstEII GGTNACC 1 cut(s) 369
BstF5I GGATG 2 cut(s) 236, 434
BstFNI CGCG 2 cut(s) 519, 521
BstHHI GCGC 1 cut(s) 521
BstKTI GATC 4 cut(s) 22, 60, 64, 501
BstMAI GTCTC 1 cut(s) 588
BstMBI GATC 4 cut(s) 19, 57, 61, 498
BstMWI GCNNNNNNNGC 3 cut(s) 201, 242, 289
BstNI CCWGG 1 cut(s) 132
BstNSI RCATGY 1 cut(s) 190
BstPI GGTNACC 1 cut(s) 369
BstSCI CCNGG 1 cut(s) 130
BstSFI CTRYAG 1 cut(s) 199
BstSLI GKGCMC 1 cut(s) 238
BstUI CGCG 2 cut(s) 519, 521
BstV1I GCAGC 3 cut(s) 182, 229, 276
BstX2I RGATCY 1 cut(s) 498
BstYI RGATCY 1 cut(s) 498
Bsu15I ATCGAT 1 cut(s) 60
BsuRI GGCC 3 cut(s) 236, 405, 488
BsuTUI ATCGAT 1 cut(s) 60
BtgI CCRYGG 1 cut(s) 575
BtsCI GGATG 2 cut(s) 236, 434
BtsI GCAGTG 1 cut(s) 331
BtsIMutI CAGTG 2 cut(s) 331, 471
Cac8I GCNNGC 2 cut(s) 148, 591
CciI TCATGA 1 cut(s) 118
CfoI GCGC 1 cut(s) 521
Cfr10I RCCGGY 1 cut(s) 511
Cfr13I GGNCC 5 cut(s) 128, 234, 235, 514, 566
ClaI ATCGAT 1 cut(s) 60
CpoI CGGWCCG 1 cut(s) 514
CspAI ACCGGT 1 cut(s) 511
CspI CGGWCCG 1 cut(s) 514
CviAII CATG 4 cut(s) 7, 119, 187, 576
DdeI CTNAG 2 cut(s) 382, 547
DpnI GATC 4 cut(s) 21, 59, 63, 500
DpnII GATC 4 cut(s) 19, 57, 61, 498
DriI GACNNNNNGTC 1 cut(s) 512
Eam1105I GACNNNNNGTC 1 cut(s) 512
Eco130I CCWWGG 3 cut(s) 139, 175, 575
Eco147I AGGCCT 1 cut(s) 405
Eco24I GRGCYC 1 cut(s) 238
Eco47I GGWCC 3 cut(s) 128, 514, 566
Eco91I GGTNACC 1 cut(s) 369
EcoO109I RGGNCCY 2 cut(s) 235, 566
EcoO65I GGTNACC 1 cut(s) 369
EcoRII CCWGG 1 cut(s) 130
EcoT14I CCWWGG 3 cut(s) 139, 175, 575
EcoT38I GRGCYC 1 cut(s) 238
ErhI CCWWGG 3 cut(s) 139, 175, 575
FaeI CATG 4 cut(s) 10, 122, 190, 579
FaqI GGGAC 3 cut(s) 141, 519, 579
FatI CATG 4 cut(s) 6, 118, 186, 575
Fnu4HI GCNGC 5 cut(s) 196, 243, 290, 293, 522
FokI GGATG 2 cut(s) 243, 421
FriOI GRGCYC 1 cut(s) 238
Fsp4HI GCNGC 5 cut(s) 196, 243, 290, 293, 522
FspBI CTAG 2 cut(s) 77, 417
GlaI GCGC 1 cut(s) 520
GluI GCNGC 5 cut(s) 196, 243, 290, 293, 522
GsaI CCCAGC 1 cut(s) 557
HaeIII GGCC 3 cut(s) 236, 405, 488
HapII CCGG 2 cut(s) 425, 512
HhaI GCGC 1 cut(s) 521
Hin1II CATG 4 cut(s) 10, 122, 190, 579
Hin6I GCGC 1 cut(s) 519
HinP1I GCGC 1 cut(s) 519
HindIII AAGCTT 1 cut(s) 148
HinfI GANTC 3 cut(s) 180, 491, 530
HpaII CCGG 2 cut(s) 425, 512
HphI GGTGA 1 cut(s) 156
Hpy188I TCNGA 2 cut(s) 385, 550
Hpy188III TCNNGA 4 cut(s) 119, 334, 343, 425
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 2 cut(s) 171, 285
HpyF10VI GCNNNNNNNGC 3 cut(s) 201, 242, 289
HpyF3I CTNAG 2 cut(s) 382, 547
Hsp92II CATG 4 cut(s) 10, 122, 190, 579
HspAI GCGC 1 cut(s) 519
KflI GGGWCCC 1 cut(s) 566
Kpn2I TCCGGA 1 cut(s) 424
Kzo9I GATC 4 cut(s) 19, 57, 61, 498
LmnI GCTCC 1 cut(s) 321
Lsp1109I GCAGC 3 cut(s) 182, 229, 276
MaeI CTAG 2 cut(s) 77, 417
MaeIII GTNAC 2 cut(s) 369, 412
MalI GATC 4 cut(s) 21, 59, 63, 500
MboI GATC 4 cut(s) 19, 57, 61, 498
MboII GAAGA 2 cut(s) 145, 270
MflI RGATCY 1 cut(s) 498
MhlI GDGCHC 1 cut(s) 238
MlyI GAGTC 1 cut(s) 485
MnlI CCTC 7 cut(s) 62, 115, 391, 449, 478, 479, 556
MroI TCCGGA 1 cut(s) 424
MspA1I CMGCKG 1 cut(s) 589
MspI CCGG 2 cut(s) 425, 512
MspR9I CCNGG 1 cut(s) 132
MvaI CCWGG 1 cut(s) 132
MvnI CGCG 2 cut(s) 519, 521
MwoI GCNNNNNNNGC 3 cut(s) 201, 242, 289
NcoI CCATGG 1 cut(s) 575
NdeII GATC 4 cut(s) 19, 57, 61, 498
NlaIII CATG 4 cut(s) 10, 122, 190, 579
NlaIV GGNNCC 4 cut(s) 129, 236, 567, 568
NmuCI GTSAC 1 cut(s) 412
NspI RCATGY 1 cut(s) 190
NspV TTCGAA 1 cut(s) 156
PagI TCATGA 1 cut(s) 118
PceI AGGCCT 1 cut(s) 405
PfeI GAWTC 2 cut(s) 180, 530
PflMI CCANNNNNTGG 1 cut(s) 576
PinAI ACCGGT 1 cut(s) 511
PkrI GCNGC 5 cut(s) 197, 244, 291, 294, 523
PleI GAGTC 1 cut(s) 485
PpsI GAGTC 1 cut(s) 485
PpuMI RGGWCCY 1 cut(s) 566
PsiI TTATAA 1 cut(s) 393
Psp5II RGGWCCY 1 cut(s) 566
Psp6I CCWGG 1 cut(s) 130
PspEI GGTNACC 1 cut(s) 369
PspFI CCCAGC 1 cut(s) 553
PspGI CCWGG 1 cut(s) 130
PspN4I GGNNCC 4 cut(s) 129, 236, 567, 568
PspOMI GGGCCC 1 cut(s) 234
PspPI GGNCC 5 cut(s) 128, 234, 235, 514, 566
PspPPI RGGWCCY 1 cut(s) 566
PsuI RGATCY 1 cut(s) 498
Rsr2I CGGWCCG 1 cut(s) 514
RsrII CGGWCCG 1 cut(s) 514
SatI GCNGC 5 cut(s) 196, 243, 290, 293, 522
Sau3AI GATC 4 cut(s) 19, 57, 61, 498
Sau96I GGNCC 5 cut(s) 128, 234, 235, 514, 566
SchI GAGTC 1 cut(s) 485
ScrFI CCNGG 1 cut(s) 132
SduI GDGCHC 1 cut(s) 238
SetI ASST 6 cut(s) 37, 141, 152, 163, 422, 455
SfcI CTRYAG 1 cut(s) 199
SfuI TTCGAA 1 cut(s) 156
SinI GGWCC 3 cut(s) 128, 514, 566
SpeI ACTAGT 1 cut(s) 76
SseBI AGGCCT 1 cut(s) 405
SsiI CCGC 6 cut(s) 228, 292, 378, 517, 521, 587
SspMI CTAG 2 cut(s) 77, 417
StuI AGGCCT 1 cut(s) 405
StyD4I CCNGG 1 cut(s) 130
StyI CCWWGG 3 cut(s) 139, 175, 575
TaqI TCGA 3 cut(s) 60, 156, 441
TauI GCSGC 2 cut(s) 295, 524
TfiI GAWTC 2 cut(s) 180, 530
TscAI CASTG 2 cut(s) 331, 478
TseFI GTSAC 1 cut(s) 412
TseI GCWGC 3 cut(s) 195, 242, 289
Tsp45I GTSAC 1 cut(s) 412
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 331, 478
Van91I CCANNNNNTGG 1 cut(s) 576
VpaK11BI GGWCC 3 cut(s) 128, 514, 566
XceI RCATGY 1 cut(s) 190
XspI CTAG 2 cut(s) 77, 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.