Rh1BG030400

Vacuolar protein sorting-associated protein 62

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0009493
Physical Location & Seq
Forward (+)
4084462 .. 4087146
2685 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG030400.1

Sequence Viewer

Length: 1659 bp
ATGGGAAATTGCCTTTCCCTCTCATGTTCAACCAAGAACATCATGGCGAATCCGATCGAAACCACATTCAAGCTTCCCTCTCCATTACCCAGTTGGCCATCTGGTAGTGAGTTCGCAAATGGAATCATACATCTGGGAGGATTACAAGTATGCCAGATTTCATCTTTCAACAAAGTATGGGCAACCCATGAGGGAGGACCAGACAACCTTGGGGCAACCTTCTTCGAACCTTCACCCCTACCCCAAGGATTCTATATGCTCGGTTGCTACAGCCAACCCAACAACAAACCCCTTTTCGGATGGGCCCTTGTTGCCAAAGCCGACGATCAGTCCTCCTCTCCATGGCCGGCTTTACAGCAACCAGTTGATTACACTCTGGTATGGAGCAGCGAGTCCGTGAAGATGAAGAAAGATGGTGATGGATACCTCTGGTTACCCACTGCCCCGGAGCGTTATGCAGCCATAGGCCATGTTGTCACTACCTCTCCTGAGAAGCCGTCCCTTGATAAAATCAGGTGCGTTCGATCTGACCTTACTGACCAATGTGAAGCTGACTCGTGGATCTGGGGAACCCCTGGTGGCACCATTGATGGGTTCAATGTATATAGTCTCACACCCAGCAATAGGGGCATCCAAGCTATCGGTGTCCCCGTTGGCACATTCCTAGCCAGTAATGGTAAAGCAAATAATATAGCTTGTTTGAAAAACAATAACACCCAACCACACACTTGTATGCCAAACCAAAGCCAAATTGTTGCAATACTTCAAGCTTACGCTCCATTCATATACTTCCATCCCGATGAAGTTTACCTCCCTTCTTCGGTGGGTTGGTTTTTCACAAATGGAGCACTTCTGTATAGCAAGGAACAAGTAGAAAATCCCGTTCCAATTGAACCCACTGGCTCTAACCTTCCGCAAGGTGGCTCAAATGATGGCGCCTATTGGTTGGACCTACCGGTCGATCAAGCAGCCAAAGAAAGAGTGAAAAAGGGAGATCTTGTTGACGCCGAGGCCTACTTACACATAAAACCCATGTTAGGCTCAACATTCACTGACATAGCTATTTGGGTCTTCTACCCTTTCAATGGCCCCGCAAGGGCCAAAGTTGAGTTCTTCAATGTTTCGTTGGGGAAGATAGGTGAGCATGTTGGTGACTGGGAGCATGTCACATTAAGAGTAAGCAATTTCACTGGCCAACTCTGGAGTGTCTACTTCTCGGAACACAGTGGGGGAATATGGGTGGACGCTTCTGGGGTTGAATTTCAAAATGGCAACAAACCAGTGACATATGCATCTTTGCATGGACATGCCTTCTATTCCAAACCTGGACTTGTTTTGCAAGGGAATGGAAGCATAGGAATTAGGAATGATACTGCAAAGAGTAAAATGGTAATGGACTCTGGACTAAGGCCTTCAATAATTGCAGCCGAGTATCTGGGCATCATTGAACCACCATGGCTTAACTACAGCAGTAAATGGGGTCCGAAAATTAGTTATCAGATTTCGGATGAAATAAAAAAGGTGGAGAAGCTCTTGCCTGGAAAGCTCAAGACTGTTTTTGACAAGTTTATCAAAAGTCTACCAGATGAAGTATTGGGAGAAGAAGGGCCTACAGGTCCCAAGATGAAGAATAACTGGAATGGAGATGAAGTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps62

Protein Analysis

552

Amino Acids

60.41

Weight (kDa)

5.62

Isoelectric Point (pI)

43.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps62 PF06101 20 - 550 3.6e-252 Vacuolar protein sorting-associated protein 62
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 956
AccB1I GGYRCC 2 cut(s) 581, 935
AccI GTMKAC 2 cut(s) 1209, 1579
AciI CCGC 2 cut(s) 914, 1092
AclWI GGATC 1 cut(s) 569
AcoI YGGCCR 3 cut(s) 95, 344, 1192
AcsI RAATTY 1 cut(s) 1259
AcyI GRCGYC 2 cut(s) 936, 1005
AfiI CCNNNNNNNGG 9 cut(s) 296, 342, 591, 624, 820, 920, 1037, 1085, 1096
AgeI ACCGGT 1 cut(s) 955
AhdI GACNNNNNGTC 1 cut(s) 328
AjnI CCWGG 3 cut(s) 574, 1324, 1537
AjuI GAANNNNNNNTTGG 2 cut(s) 1094, 1126
AluBI AGCT 8 cut(s) 73, 551, 638, 695, 770, 1061, 1531, 1546
AluI AGCT 8 cut(s) 73, 551, 638, 695, 770, 1061, 1531, 1546
Alw21I GWGCWC 1 cut(s) 850
Alw26I GTCTC 1 cut(s) 614
AlwI GGATC 1 cut(s) 569
ApaI GGGCCC 1 cut(s) 307
ApeKI GCWGC 4 cut(s) 387, 458, 968, 1424
ApoI RAATTY 1 cut(s) 1259
AsiGI ACCGGT 1 cut(s) 955
AspLEI GCGC 1 cut(s) 938
AspS9I GGNCC 9 cut(s) 197, 303, 304, 949, 1088, 1098, 1481, 1607, 1616
AsuC2I CCSGG 1 cut(s) 446
AsuHPI GGTGA 4 cut(s) 225, 428, 1151, 1163
AsuII TTCGAA 1 cut(s) 225
AvaII GGWCC 4 cut(s) 197, 949, 1481, 1616
BaeGI GKGCMC 1 cut(s) 307
BalI TGGCCA 2 cut(s) 97, 1194
BanI GGYRCC 2 cut(s) 581, 935
BanII GRGCYC 1 cut(s) 307
BauI CACGAG 1 cut(s) 556
BbsI GAAGAC 1 cut(s) 1063
Bbv12I GWGCWC 1 cut(s) 850
BbvI GCAGC 4 cut(s) 399, 470, 980, 1436
BccI CCATC 7 cut(s) 106, 294, 407, 413, 584, 801, 926
BceAI ACGGC 1 cut(s) 481
BciT130I CCWGG 3 cut(s) 576, 1326, 1539
BciVI GTATCC 1 cut(s) 416
BcnI CCSGG 1 cut(s) 446
BcoDI GTCTC 1 cut(s) 614
BfaI CTAG 1 cut(s) 665
BfmI CTRYAG 3 cut(s) 268, 1465, 1611
BfoI RGCGCY 1 cut(s) 939
BfuI GTATCC 1 cut(s) 416
BglII AGATCT 1 cut(s) 994
BisI GCNGC 4 cut(s) 388, 459, 969, 1425
BlsI GCNGC 4 cut(s) 389, 460, 970, 1426
Bme1390I CCNGG 4 cut(s) 446, 576, 1326, 1539
Bme18I GGWCC 4 cut(s) 197, 949, 1481, 1616
BmeRI GACNNNNNGTC 1 cut(s) 328
BmgT120I GGNCC 9 cut(s) 197, 303, 304, 949, 1088, 1098, 1481, 1607, 1616
BmiI GGNNCC 7 cut(s) 305, 571, 583, 937, 1090, 1482, 1618
BmrFI CCNGG 4 cut(s) 446, 576, 1326, 1539
BmrI ACTGGG 2 cut(s) 84, 1165
BmsI GCATC 3 cut(s) 639, 1301, 1449
BmuI ACTGGG 2 cut(s) 84, 1165
BpiI GAAGAC 1 cut(s) 1063
BpmI CTGGAG 1 cut(s) 1222
Bpu14I TTCGAA 1 cut(s) 225
BpuEI CTTGAG 1 cut(s) 1532
BpuMI CCSGG 1 cut(s) 446
BsaHI GRCGYC 2 cut(s) 936, 1005
BsaJI CCNNGG 7 cut(s) 208, 244, 341, 444, 574, 1008, 1454
BsaWI WCCGGW 1 cut(s) 955
BsaXI ACNNNNNCTCC 5 cut(s) 469, 499, 984, 1014, 1635
Bsc4I CCNNNNNNNGG 9 cut(s) 296, 342, 591, 624, 820, 920, 1037, 1085, 1096
Bse118I RCCGGY 2 cut(s) 346, 955
Bse1I ACTGG 8 cut(s) 90, 362, 669, 904, 1160, 1195, 1280, 1640
BseBI CCWGG 3 cut(s) 576, 1326, 1539
BseDI CCNNGG 7 cut(s) 208, 244, 341, 444, 574, 1008, 1454
BseGI GGATG 4 cut(s) 305, 630, 793, 1513
BseLI CCNNNNNNNGG 9 cut(s) 296, 342, 591, 624, 820, 920, 1037, 1085, 1096
BseMII CTCAG 1 cut(s) 480
BseNI ACTGG 8 cut(s) 90, 362, 669, 904, 1160, 1195, 1280, 1640
BseRI GAGGAG 1 cut(s) 325
BseSI GKGCMC 1 cut(s) 307
BseXI GCAGC 4 cut(s) 399, 470, 980, 1436
BseYI CCCAGC 1 cut(s) 617
Bsh1285I CGRYCG 2 cut(s) 57, 960
BshNI GGYRCC 2 cut(s) 581, 935
BshTI ACCGGT 1 cut(s) 955
BsiEI CGRYCG 2 cut(s) 57, 960
BsiHKAI GWGCWC 1 cut(s) 850
BsiSI CCGG 3 cut(s) 347, 446, 956
BslFI GGGAC 3 cut(s) 484, 632, 1602
BslI CCNNNNNNNGG 9 cut(s) 296, 342, 591, 624, 820, 920, 1037, 1085, 1096
BsmAI GTCTC 1 cut(s) 614
BsmFI GGGAC 3 cut(s) 484, 632, 1602
Bsp119I TTCGAA 1 cut(s) 225
Bsp120I GGGCCC 1 cut(s) 303
Bsp1286I GDGCHC 2 cut(s) 307, 850
Bsp143I GATC 6 cut(s) 54, 325, 524, 561, 961, 994
Bsp19I CCATGG 2 cut(s) 341, 1454
BspACI CCGC 2 cut(s) 914, 1092
BspCNI CTCAG 1 cut(s) 481
BspLI GGNNCC 7 cut(s) 305, 571, 583, 937, 1090, 1482, 1618
BspPI GGATC 1 cut(s) 569
BspT104I TTCGAA 1 cut(s) 225
BspT107I GGYRCC 2 cut(s) 581, 935
BsrFI RCCGGY 2 cut(s) 346, 955
BsrI ACTGG 8 cut(s) 90, 362, 669, 904, 1160, 1195, 1280, 1640
BssAI RCCGGY 2 cut(s) 346, 955
BssECI CCNNGG 7 cut(s) 208, 244, 341, 444, 574, 1008, 1454
BssMI GATC 6 cut(s) 54, 325, 524, 561, 961, 994
BssNI GRCGYC 2 cut(s) 936, 1005
BssSI CACGAG 1 cut(s) 556
BssT1I CCWWGG 4 cut(s) 208, 244, 341, 1454
Bst2BI CACGAG 1 cut(s) 556
Bst2UI CCWGG 3 cut(s) 576, 1326, 1539
Bst4CI ACNGT 2 cut(s) 1226, 1555
BstACI GRCGYC 2 cut(s) 936, 1005
BstBI TTCGAA 1 cut(s) 225
BstC8I GCNNGC 1 cut(s) 348
BstDEI CTNAG 2 cut(s) 489, 1406
BstDSI CCRYGG 2 cut(s) 341, 1454
BstEII GGTNACC 1 cut(s) 432
BstF5I GGATG 4 cut(s) 305, 630, 793, 1513
BstH2I RGCGCY 1 cut(s) 939
BstHHI GCGC 1 cut(s) 938
BstKTI GATC 6 cut(s) 57, 328, 527, 564, 964, 997
BstMAI GTCTC 1 cut(s) 614
BstMBI GATC 6 cut(s) 54, 325, 524, 561, 961, 994
BstMCI CGRYCG 2 cut(s) 57, 960
BstMWI GCNNNNNNNGC 3 cut(s) 311, 627, 1543
BstNI CCWGG 3 cut(s) 576, 1326, 1539
BstNSI RCATGY 3 cut(s) 1148, 1166, 1310
BstPI GGTNACC 1 cut(s) 432
BstSCI CCNGG 4 cut(s) 444, 574, 1324, 1537
BstSFI CTRYAG 3 cut(s) 268, 1465, 1611
BstSLI GKGCMC 1 cut(s) 307
BstV1I GCAGC 4 cut(s) 399, 470, 980, 1436
BstV2I GAAGAC 1 cut(s) 1063
BstX2I RGATCY 2 cut(s) 561, 994
BstYI RGATCY 2 cut(s) 561, 994
BsuI GTATCC 1 cut(s) 416
BtgI CCRYGG 2 cut(s) 341, 1454
BtsCI GGATG 4 cut(s) 305, 630, 793, 1513
BtsI GCAGTG 1 cut(s) 438
BtsIMutI CAGTG 6 cut(s) 438, 897, 1050, 1188, 1231, 1287
Cac8I GCNNGC 1 cut(s) 348
CfoI GCGC 1 cut(s) 938
Cfr10I RCCGGY 2 cut(s) 346, 955
Cfr13I GGNCC 9 cut(s) 197, 303, 304, 949, 1088, 1098, 1481, 1607, 1616
CseI GACGC 2 cut(s) 1013, 1253
CspAI ACCGGT 1 cut(s) 955
DdeI CTNAG 2 cut(s) 489, 1406
DinI GGCGCC 1 cut(s) 937
DpnI GATC 6 cut(s) 56, 327, 526, 563, 963, 996
DpnII GATC 6 cut(s) 54, 325, 524, 561, 961, 994
DrdI GACNNNNNNGTC 1 cut(s) 956
DriI GACNNNNNGTC 1 cut(s) 328
DseDI GACNNNNNNGTC 1 cut(s) 956
EaeI YGGCCR 3 cut(s) 95, 344, 1192
Eam1105I GACNNNNNGTC 1 cut(s) 328
Eco130I CCWWGG 4 cut(s) 208, 244, 341, 1454
Eco147I AGGCCT 2 cut(s) 1013, 1411
Eco24I GRGCYC 1 cut(s) 307
Eco47I GGWCC 4 cut(s) 197, 949, 1481, 1616
Eco91I GGTNACC 1 cut(s) 432
EcoO109I RGGNCCY 3 cut(s) 304, 1607, 1616
EcoO65I GGTNACC 1 cut(s) 432
EcoRII CCWGG 3 cut(s) 574, 1324, 1537
EcoT14I CCWWGG 4 cut(s) 208, 244, 341, 1454
EcoT22I ATGCAT 1 cut(s) 1294
EcoT38I GRGCYC 1 cut(s) 307
EgeI GGCGCC 1 cut(s) 937
EheI GGCGCC 1 cut(s) 937
ErhI CCWWGG 4 cut(s) 208, 244, 341, 1454
FaqI GGGAC 3 cut(s) 484, 632, 1602
FauI CCCGC 1 cut(s) 1099
FauNDI CATATG 1 cut(s) 1288
FblI GTMKAC 2 cut(s) 1209, 1579
Fnu4HI GCNGC 4 cut(s) 388, 459, 969, 1425
FokI GGATG 4 cut(s) 312, 617, 780, 1520
FriOI GRGCYC 1 cut(s) 307
Fsp4HI GCNGC 4 cut(s) 388, 459, 969, 1425
FspBI CTAG 1 cut(s) 665
GlaI GCGC 1 cut(s) 937
GluI GCNGC 4 cut(s) 388, 459, 969, 1425
GsaI CCCAGC 1 cut(s) 621
GsuI CTGGAG 1 cut(s) 1222
HaeII RGCGCY 1 cut(s) 939
HapII CCGG 3 cut(s) 347, 446, 956
HgaI GACGC 2 cut(s) 1013, 1253
HhaI GCGC 1 cut(s) 938
Hin1I GRCGYC 2 cut(s) 936, 1005
Hin6I GCGC 1 cut(s) 936
HinP1I GCGC 1 cut(s) 936
HincII GTYRAC 1 cut(s) 1003
HindII GTYRAC 1 cut(s) 1003
HindIII AAGCTT 2 cut(s) 71, 768
HinfI GANTC 6 cut(s) 49, 123, 249, 392, 554, 1397
HpaII CCGG 3 cut(s) 347, 446, 956
HphI GGTGA 4 cut(s) 225, 428, 1151, 1163
Hpy166II GTNNAC 5 cut(s) 808, 1003, 1210, 1243, 1580
Hpy188I TCNGA 7 cut(s) 54, 299, 529, 1219, 1485, 1500, 1507
Hpy188III TCNNGA 5 cut(s) 488, 797, 1201, 1401, 1549
Hpy8I GTNNAC 5 cut(s) 808, 1003, 1210, 1243, 1580
Hpy99I CGWCG 1 cut(s) 326
HpyAV CCTTC 7 cut(s) 229, 240, 825, 920, 1321, 1422, 1598
HpyCH4III ACNGT 2 cut(s) 1226, 1555
HpyCH4V TGCA 7 cut(s) 458, 758, 1292, 1300, 1339, 1376, 1424
HpyF10VI GCNNNNNNNGC 3 cut(s) 311, 627, 1543
HpyF3I CTNAG 2 cut(s) 489, 1406
Hsp92I GRCGYC 2 cut(s) 936, 1005
HspAI GCGC 1 cut(s) 936
KasI GGCGCC 1 cut(s) 935
KroI GCCGGC 1 cut(s) 346
KroNI GCCGGC 1 cut(s) 348
Kzo9I GATC 6 cut(s) 54, 325, 524, 561, 961, 994
LmnI GCTCC 5 cut(s) 384, 448, 781, 845, 1159
Lsp1109I GCAGC 4 cut(s) 399, 470, 980, 1436
LweI GCATC 3 cut(s) 639, 1301, 1449
MaeI CTAG 1 cut(s) 665
MaeIII GTNAC 5 cut(s) 432, 475, 1151, 1165, 1282
MalI GATC 6 cut(s) 56, 327, 526, 563, 963, 996
MboI GATC 6 cut(s) 54, 325, 524, 561, 961, 994
MboII GAAGA 9 cut(s) 214, 412, 418, 810, 1063, 1105, 1144, 1613, 1639
MfeI CAATTG 1 cut(s) 888
MflI RGATCY 2 cut(s) 561, 994
MhlI GDGCHC 2 cut(s) 307, 850
MlsI TGGCCA 2 cut(s) 97, 1194
MluCI AATT 8 cut(s) 7, 750, 888, 1183, 1259, 1359, 1419, 1488
MluNI TGGCCA 2 cut(s) 97, 1194
Mly113I GGCGCC 1 cut(s) 936
MlyI GAGTC 3 cut(s) 401, 548, 1391
MmeI TCCRAC 1 cut(s) 927
Mox20I TGGCCA 2 cut(s) 97, 1194
Mph1103I ATGCAT 1 cut(s) 1294
MroNI GCCGGC 1 cut(s) 346
MscI TGGCCA 2 cut(s) 97, 1194
MseI TTAA 2 cut(s) 1172, 1461
MslI CAYNNNNRTG 4 cut(s) 731, 798, 1149, 1305
Msp20I TGGCCA 2 cut(s) 97, 1194
MspI CCGG 3 cut(s) 347, 446, 956
MspR9I CCNGG 4 cut(s) 446, 576, 1326, 1539
MunI CAATTG 1 cut(s) 888
MvaI CCWGG 3 cut(s) 576, 1326, 1539
MwoI GCNNNNNNNGC 3 cut(s) 311, 627, 1543
NaeI GCCGGC 1 cut(s) 348
NarI GGCGCC 1 cut(s) 936
NciI CCSGG 1 cut(s) 446
NcoI CCATGG 2 cut(s) 341, 1454
NdeI CATATG 1 cut(s) 1288
NdeII GATC 6 cut(s) 54, 325, 524, 561, 961, 994
NgoMIV GCCGGC 1 cut(s) 346
NlaIV GGNNCC 7 cut(s) 305, 571, 583, 937, 1090, 1482, 1618
NmeAIII GCCGAG 2 cut(s) 1033, 1453
NmuCI GTSAC 4 cut(s) 475, 1151, 1165, 1282
NsiI ATGCAT 1 cut(s) 1294
NspI RCATGY 3 cut(s) 1148, 1166, 1310
NspV TTCGAA 1 cut(s) 225
PceI AGGCCT 2 cut(s) 1013, 1411
PcsI WCGNNNNNNNCGW 1 cut(s) 648
PdiI GCCGGC 1 cut(s) 348
PfeI GAWTC 3 cut(s) 49, 123, 249
PinAI ACCGGT 1 cut(s) 955
PkrI GCNGC 4 cut(s) 389, 460, 970, 1426
Ple19I CGATCG 1 cut(s) 57
PleI GAGTC 3 cut(s) 400, 548, 1391
PluTI GGCGCC 1 cut(s) 939
PpsI GAGTC 3 cut(s) 400, 548, 1391
PpuMI RGGWCCY 1 cut(s) 1616
Psp5II RGGWCCY 1 cut(s) 1616
Psp6I CCWGG 3 cut(s) 574, 1324, 1537
PspEI GGTNACC 1 cut(s) 432
PspFI CCCAGC 1 cut(s) 617
PspGI CCWGG 3 cut(s) 574, 1324, 1537
PspN4I GGNNCC 7 cut(s) 305, 571, 583, 937, 1090, 1482, 1618
PspOMI GGGCCC 1 cut(s) 303
PspPI GGNCC 9 cut(s) 197, 303, 304, 949, 1088, 1098, 1481, 1607, 1616
PspPPI RGGWCCY 1 cut(s) 1616
PsuI RGATCY 2 cut(s) 561, 994
PvuI CGATCG 1 cut(s) 57
RseI CAYNNNNRTG 4 cut(s) 731, 798, 1149, 1305
SaqAI TTAA 2 cut(s) 1172, 1461
SatI GCNGC 4 cut(s) 388, 459, 969, 1425
Sau3AI GATC 6 cut(s) 54, 325, 524, 561, 961, 994
Sau96I GGNCC 9 cut(s) 197, 303, 304, 949, 1088, 1098, 1481, 1607, 1616
SchI GAGTC 3 cut(s) 401, 548, 1391
ScrFI CCNGG 4 cut(s) 446, 576, 1326, 1539
SduI GDGCHC 2 cut(s) 307, 850
SfaNI GCATC 3 cut(s) 639, 1301, 1449
SfcI CTRYAG 3 cut(s) 268, 1465, 1611
SfoI GGCGCC 1 cut(s) 937
SfuI TTCGAA 1 cut(s) 225
SinI GGWCC 4 cut(s) 197, 949, 1481, 1616
SmiMI CAYNNNNRTG 4 cut(s) 731, 798, 1149, 1305
SmlI CTYRAG 1 cut(s) 1547
SmoI CTYRAG 1 cut(s) 1547
Sse9I AATT 8 cut(s) 7, 750, 888, 1183, 1259, 1359, 1419, 1488
SseBI AGGCCT 2 cut(s) 1013, 1411
SsiI CCGC 2 cut(s) 914, 1092
SspDI GGCGCC 1 cut(s) 935
SspMI CTAG 1 cut(s) 665
StuI AGGCCT 2 cut(s) 1013, 1411
StyD4I CCNGG 4 cut(s) 444, 574, 1324, 1537
StyI CCWWGG 4 cut(s) 208, 244, 341, 1454
TaaI ACNGT 2 cut(s) 1226, 1555
TaqI TCGA 4 cut(s) 57, 225, 523, 960
TasI AATT 8 cut(s) 7, 750, 888, 1183, 1259, 1359, 1419, 1488
TfiI GAWTC 3 cut(s) 49, 123, 249
Tru1I TTAA 2 cut(s) 1172, 1461
Tru9I TTAA 2 cut(s) 1172, 1461
TscAI CASTG 6 cut(s) 445, 904, 1057, 1195, 1231, 1287
TseFI GTSAC 4 cut(s) 475, 1151, 1165, 1282
TseI GCWGC 4 cut(s) 387, 458, 968, 1424
Tsp45I GTSAC 4 cut(s) 475, 1151, 1165, 1282
TspDTI ATGAA 7 cut(s) 150, 419, 772, 816, 1524, 1602, 1640
TspGWI ACGGA 1 cut(s) 385
TspRI CASTG 6 cut(s) 445, 904, 1057, 1195, 1231, 1287
VpaK11BI GGWCC 4 cut(s) 197, 949, 1481, 1616
XapI RAATTY 1 cut(s) 1259
XceI RCATGY 3 cut(s) 1148, 1166, 1310
XcmI CCANNNNNNNNNTGG 2 cut(s) 40, 90
XmiI GTMKAC 2 cut(s) 1209, 1579
XspI CTAG 1 cut(s) 665
Zsp2I ATGCAT 1 cut(s) 1294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.