AT2G45040

Belongs to the peptidase M10A family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0016658
Physical Location & Seq
Forward (+)
18577500 .. 18578945
1446 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G45040.1

Sequence Viewer

Length: 1029 bp
ATGCATCATCATCATCATCCATGCAATCGCAAACCCTTCACAACCATCTTCTCCTTCTTCCTTCTCTATCTCAATCTCCATAACCAACAAATTATCGAAGCTCGAAATCCATCTCAATTTACGACCAACCCATCCCCTGACGTCTCTATACCGGAAATCAAACGTCACCTCCAACAGTACGGTTACCTCCCACAAAACAAAGAATCCGACGACGTTTCGTTCGAACAAGCTCTCGTTCGTTACCAGAAAAATCTCGGTCTACCAATAACCGGTAAACCGGATTCCGACACGTTGTCACAGATTCTATTACCAAGATGTGGGTTCCCTGATGACGTGGAACCCAAAACGGCACCGTTTCACACAGGAAAAAAATACGTGTACTTCCCCGGACGGCCCAGGTGGACAAGAGACGTCCCACTGAAACTCACGTACGCCTTCTCGCAGGAGAACCTAACTCCTTACTTAGCACCAACTGACATACGGCGCGTGTTCCGACGCGCTTTCGGCAAGTGGGCTTCGGTGATCCCAGTATCCTTCATCGAAACAGAGGATTACGTCATCGCCGACATCAAGATCGGATTCTTCAACGGAGATCACGGCGACGGAGAACCGTTCGACGGCGTTTTAGGTGTTTTAGCACACACTTTCTCTCCGGAAAACGGTAGGCTTCATCTAGACAAAGCAGAGACGTGGGCCGTAGATTTCGATGAGGAAAAATCTAGTGTGGCCGTCGATTTGGAATCTGTGGCGGTGCACGAGATCGGTCACGTGCTTGGATTAGGACATAGCTCGGTGAAAGACGCGGCTATGTATCCAACTTTAAAGCCAAGAAGTAAGAAAGTGAATTTGAATATGGACGACGTCGTTGGAGTACAGTCTTTGTACGGAACAAACCCTAATTTCACGTTAAATAGTTTACTTGCGTCGGAAACTTCGACAAATCTAGCCGATGGTTCGAGGATACGGTCTCAAGGAATGATCTATTCGACTTTGAGTACTGTTATCGCTCTCTGTTTTCTTAATTGGTAG

Protein Analysis

342

Amino Acids

38.51

Weight (kDa)

6.26

Isoelectric Point (pI)

39.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 50 - 100 4.5e-09 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 134 - 296 1.2e-50 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016658)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45040
fragaria_vesca FvH4_7g10690
malus_domestica MD00G1063700.v1.1
prunus_persica Prupe.2G135100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0348631
rosa_laevigata RLG00000028634
rosa_multiflora Rmu_sc0004109.1_g000009
rosa_roxburghii Rroxscaffold_4G00306870
rosa_rugosa Rorug01G0195900
rosa_samantha Rh1AG213600 Rh1BG180100 Rh1CG198200 Rh1DG210000
rosa_wichuraiana Rw1G018090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 144, 414, 864
AccB1I GGYRCC 1 cut(s) 349
AccB7I CCANNNNNTGG 1 cut(s) 317
AccI GTMKAC 1 cut(s) 259
AccII CGCG 3 cut(s) 486, 498, 803
AccIII TCCGGA 1 cut(s) 652
AciI CCGC 2 cut(s) 749, 803
AclWI GGATC 1 cut(s) 517
AcoI YGGCCR 1 cut(s) 726
AcsI RAATTY 1 cut(s) 844
AcvI CACGTG 1 cut(s) 769
AcyI GRCGYC 3 cut(s) 141, 411, 861
AfaI GTAC 6 cut(s) 179, 380, 431, 873, 884, 997
AfiI CCNNNNNNNGG 4 cut(s) 269, 317, 617, 659
AflIII ACRYGT 2 cut(s) 288, 375
AgeI ACCGGT 1 cut(s) 269
AgsI TTSAA 2 cut(s) 586, 850
AhdI GACNNNNNGTC 1 cut(s) 292
AjiI CACGTC 2 cut(s) 334, 690
AjnI CCWGG 1 cut(s) 395
AluBI AGCT 3 cut(s) 101, 230, 789
AluI AGCT 3 cut(s) 101, 230, 789
Alw21I GWGCWC 1 cut(s) 756
Alw26I GTCTC 4 cut(s) 148, 402, 680, 972
Alw44I GTGCAC 1 cut(s) 752
AlwI GGATC 1 cut(s) 517
Aor13HI TCCGGA 1 cut(s) 652
AoxI GGCC 3 cut(s) 392, 693, 726
ApaLI GTGCAC 1 cut(s) 752
ApoI RAATTY 1 cut(s) 844
ArsI GACNNNNNNTTYG 2 cut(s) 203, 235
AsiGI ACCGGT 1 cut(s) 269
AspLEI GCGC 2 cut(s) 486, 500
AspS9I GGNCC 2 cut(s) 393, 693
AsuC2I CCSGG 1 cut(s) 387
AsuHPI GGTGA 3 cut(s) 158, 532, 805
AsuII TTCGAA 1 cut(s) 222
BaeGI GKGCMC 1 cut(s) 756
BanI GGYRCC 1 cut(s) 349
BauI CACGAG 1 cut(s) 755
BbrPI CACGTG 1 cut(s) 769
Bbv12I GWGCWC 1 cut(s) 756
BccI CCATC 4 cut(s) 53, 118, 139, 944
BceAI ACGGC 7 cut(s) 363, 407, 497, 613, 634, 680, 713
BciT130I CCWGG 1 cut(s) 397
BciVI GTATCC 3 cut(s) 541, 822, 954
BcnI CCSGG 1 cut(s) 387
BcoDI GTCTC 4 cut(s) 148, 402, 680, 972
BfaI CTAG 3 cut(s) 674, 720, 944
BfuI GTATCC 3 cut(s) 541, 822, 954
BisI GCNGC 1 cut(s) 804
BlsI GCNGC 1 cut(s) 805
BmcAI AGTACT 1 cut(s) 997
Bme1390I CCNGG 2 cut(s) 387, 397
BmeRI GACNNNNNGTC 1 cut(s) 292
BmgBI CACGTC 2 cut(s) 334, 690
BmgT120I GGNCC 2 cut(s) 393, 693
BmiI GGNNCC 3 cut(s) 323, 339, 351
BmrFI CCNGG 2 cut(s) 387, 397
BmrI ACTGGG 1 cut(s) 521
BmsI GCATC 1 cut(s) 13
BmuI ACTGGG 1 cut(s) 521
Bpu14I TTCGAA 1 cut(s) 222
BpuEI CTTGAG 1 cut(s) 954
BpuMI CCSGG 1 cut(s) 387
BsaAI YACGTR 3 cut(s) 376, 429, 769
BsaHI GRCGYC 3 cut(s) 141, 411, 861
BsaI GGTCTC 1 cut(s) 972
BsaJI CCNNGG 2 cut(s) 385, 395
BsaWI WCCGGW 4 cut(s) 151, 269, 277, 652
BsaXI ACNNNNNCTCC 4 cut(s) 153, 183, 634, 664
Bsc4I CCNNNNNNNGG 4 cut(s) 269, 317, 617, 659
Bse118I RCCGGY 1 cut(s) 269
Bse1I ACTGG 1 cut(s) 527
BseAI TCCGGA 1 cut(s) 652
BseBI CCWGG 1 cut(s) 397
BseDI CCNNGG 2 cut(s) 385, 395
BseGI GGATG 2 cut(s) 16, 131
BseLI CCNNNNNNNGG 4 cut(s) 269, 317, 617, 659
BseNI ACTGG 1 cut(s) 527
BseSI GKGCMC 1 cut(s) 756
Bsh1236I CGCG 3 cut(s) 486, 498, 803
BshFI GGCC 3 cut(s) 394, 695, 728
BshNI GGYRCC 1 cut(s) 349
BshTI ACCGGT 1 cut(s) 269
BsiHKAI GWGCWC 1 cut(s) 756
BsiSI CCGG 5 cut(s) 152, 270, 278, 387, 653
BsiWI CGTACG 1 cut(s) 429
BslFI GGGAC 1 cut(s) 398
BslI CCNNNNNNNGG 4 cut(s) 269, 317, 617, 659
BsmAI GTCTC 4 cut(s) 148, 402, 680, 972
BsmBI CGTCTC 3 cut(s) 148, 402, 680
BsmFI GGGAC 1 cut(s) 398
BsnI GGCC 3 cut(s) 394, 695, 728
Bso31I GGTCTC 1 cut(s) 972
Bsp119I TTCGAA 1 cut(s) 222
Bsp1286I GDGCHC 1 cut(s) 756
Bsp13I TCCGGA 1 cut(s) 652
Bsp143I GATC 5 cut(s) 522, 573, 592, 759, 978
BspACI CCGC 2 cut(s) 749, 803
BspANI GGCC 3 cut(s) 394, 695, 728
BspEI TCCGGA 1 cut(s) 652
BspFNI CGCG 3 cut(s) 486, 498, 803
BspLI GGNNCC 3 cut(s) 323, 339, 351
BspPI GGATC 1 cut(s) 517
BspT104I TTCGAA 1 cut(s) 222
BspT107I GGYRCC 1 cut(s) 349
BspTNI GGTCTC 1 cut(s) 972
BsrFI RCCGGY 1 cut(s) 269
BsrI ACTGG 1 cut(s) 527
BssAI RCCGGY 1 cut(s) 269
BssECI CCNNGG 2 cut(s) 385, 395
BssMI GATC 5 cut(s) 522, 573, 592, 759, 978
BssNI GRCGYC 3 cut(s) 141, 411, 861
BssSI CACGAG 1 cut(s) 755
Bst2BI CACGAG 1 cut(s) 755
Bst2UI CCWGG 1 cut(s) 397
Bst4CI ACNGT 8 cut(s) 177, 182, 354, 612, 662, 876, 966, 1000
BstACI GRCGYC 3 cut(s) 141, 411, 861
BstBAI YACGTR 3 cut(s) 376, 429, 769
BstBI TTCGAA 1 cut(s) 222
BstDEI CTNAG 1 cut(s) 463
BstEII GGTNACC 1 cut(s) 182
BstF5I GGATG 2 cut(s) 16, 131
BstFNI CGCG 3 cut(s) 486, 498, 803
BstHHI GCGC 2 cut(s) 486, 500
BstKTI GATC 5 cut(s) 525, 576, 595, 762, 981
BstMAI GTCTC 4 cut(s) 148, 402, 680, 972
BstMBI GATC 5 cut(s) 522, 573, 592, 759, 978
BstMWI GCNNNNNNNGC 1 cut(s) 504
BstNI CCWGG 1 cut(s) 397
BstPI GGTNACC 1 cut(s) 182
BstSCI CCNGG 2 cut(s) 385, 395
BstSLI GKGCMC 1 cut(s) 756
BstUI CGCG 3 cut(s) 486, 498, 803
BsuI GTATCC 3 cut(s) 541, 822, 954
BsuRI GGCC 3 cut(s) 394, 695, 728
BtgZI GCGATG 1 cut(s) 544
BtrI CACGTC 2 cut(s) 334, 690
BtsCI GGATG 2 cut(s) 16, 131
BtsIMutI CAGTG 1 cut(s) 416
CfoI GCGC 2 cut(s) 486, 500
Cfr10I RCCGGY 1 cut(s) 269
Cfr13I GGNCC 2 cut(s) 393, 693
CseI GACGC 3 cut(s) 504, 809, 912
Csp6I GTAC 6 cut(s) 178, 379, 430, 872, 883, 996
CspAI ACCGGT 1 cut(s) 269
CviAII CATG 1 cut(s) 21
CviQI GTAC 6 cut(s) 178, 379, 430, 872, 883, 996
DdeI CTNAG 1 cut(s) 463
DpnI GATC 5 cut(s) 524, 575, 594, 761, 980
DpnII GATC 5 cut(s) 522, 573, 592, 759, 978
DraI TTTAAA 1 cut(s) 822
DriI GACNNNNNGTC 1 cut(s) 292
EaeI YGGCCR 1 cut(s) 726
Eam1105I GACNNNNNGTC 1 cut(s) 292
Eco31I GGTCTC 1 cut(s) 972
Eco72I CACGTG 1 cut(s) 769
Eco91I GGTNACC 1 cut(s) 182
EcoO65I GGTNACC 1 cut(s) 182
EcoRII CCWGG 1 cut(s) 395
EcoT22I ATGCAT 1 cut(s) 6
Esp3I CGTCTC 3 cut(s) 148, 402, 680
FaeI CATG 1 cut(s) 24
FaiI YATR 7 cut(s) 22, 81, 149, 479, 786, 809, 854
FaqI GGGAC 1 cut(s) 398
FatI CATG 1 cut(s) 20
FblI GTMKAC 1 cut(s) 259
Fnu4HI GCNGC 1 cut(s) 804
FokI GGATG 2 cut(s) 3, 118
Fsp4HI GCNGC 1 cut(s) 804
FspBI CTAG 3 cut(s) 674, 720, 944
GlaI GCGC 2 cut(s) 485, 499
GluI GCNGC 1 cut(s) 804
HaeIII GGCC 3 cut(s) 394, 695, 728
HapII CCGG 5 cut(s) 152, 270, 278, 387, 653
HgaI GACGC 3 cut(s) 504, 809, 912
HhaI GCGC 2 cut(s) 486, 500
Hin1I GRCGYC 3 cut(s) 141, 411, 861
Hin1II CATG 1 cut(s) 24
Hin6I GCGC 2 cut(s) 484, 498
HinP1I GCGC 2 cut(s) 484, 498
HinfI GANTC 5 cut(s) 203, 281, 301, 579, 740
HpaII CCGG 5 cut(s) 152, 270, 278, 387, 653
HphI GGTGA 3 cut(s) 158, 532, 805
Hpy166II GTNNAC 6 cut(s) 260, 275, 379, 402, 754, 917
Hpy188I TCNGA 5 cut(s) 208, 286, 494, 578, 928
Hpy188III TCNNGA 3 cut(s) 571, 653, 674
Hpy8I GTNNAC 6 cut(s) 260, 275, 379, 402, 754, 917
Hpy99I CGWCG 9 cut(s) 212, 215, 498, 605, 620, 734, 863, 866, 928
HpyAV CCTTC 5 cut(s) 46, 64, 71, 445, 544
HpyCH4III ACNGT 8 cut(s) 177, 182, 354, 612, 662, 876, 966, 1000
HpyCH4V TGCA 3 cut(s) 4, 24, 754
HpyF10VI GCNNNNNNNGC 1 cut(s) 504
HpyF3I CTNAG 1 cut(s) 463
Hsp92I GRCGYC 3 cut(s) 141, 411, 861
Hsp92II CATG 1 cut(s) 24
HspAI GCGC 2 cut(s) 484, 498
Kpn2I TCCGGA 1 cut(s) 652
Kzo9I GATC 5 cut(s) 522, 573, 592, 759, 978
LweI GCATC 1 cut(s) 13
MaeI CTAG 3 cut(s) 674, 720, 944
MaeIII GTNAC 5 cut(s) 164, 182, 239, 294, 764
MalI GATC 5 cut(s) 524, 575, 594, 761, 980
MboI GATC 5 cut(s) 522, 573, 592, 759, 978
MboII GAAGA 3 cut(s) 40, 49, 574
MhlI GDGCHC 1 cut(s) 756
MluCI AATT 5 cut(s) 90, 116, 844, 898, 1021
MmeI TCCRAC 7 cut(s) 196, 231, 309, 517, 839, 847, 906
MnlI CCTC 5 cut(s) 179, 197, 541, 703, 951
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 652
MseI TTAA 3 cut(s) 821, 908, 1020
MspI CCGG 5 cut(s) 152, 270, 278, 387, 653
MspR9I CCNGG 2 cut(s) 387, 397
MvaI CCWGG 1 cut(s) 397
MvnI CGCG 3 cut(s) 486, 498, 803
MwoI GCNNNNNNNGC 1 cut(s) 504
NciI CCSGG 1 cut(s) 387
NdeII GATC 5 cut(s) 522, 573, 592, 759, 978
NlaIII CATG 1 cut(s) 24
NlaIV GGNNCC 3 cut(s) 323, 339, 351
NmuCI GTSAC 3 cut(s) 164, 294, 764
NsiI ATGCAT 1 cut(s) 6
NspV TTCGAA 1 cut(s) 222
PcsI WCGNNNNNNNCGW 3 cut(s) 219, 561, 932
PfeI GAWTC 5 cut(s) 203, 281, 301, 579, 740
Pfl23II CGTACG 1 cut(s) 429
PflFI GACNNNGTC 1 cut(s) 860
PflMI CCANNNNNTGG 1 cut(s) 317
PinAI ACCGGT 1 cut(s) 269
PkrI GCNGC 1 cut(s) 805
PmaCI CACGTG 1 cut(s) 769
PmlI CACGTG 1 cut(s) 769
Ppu21I YACGTR 3 cut(s) 376, 429, 769
Psp6I CCWGG 1 cut(s) 395
PspCI CACGTG 1 cut(s) 769
PspEI GGTNACC 1 cut(s) 182
PspGI CCWGG 1 cut(s) 395
PspLI CGTACG 1 cut(s) 429
PspN4I GGNNCC 3 cut(s) 323, 339, 351
PspPI GGNCC 2 cut(s) 393, 693
PsyI GACNNNGTC 1 cut(s) 860
RsaI GTAC 6 cut(s) 179, 380, 431, 873, 884, 997
RsaNI GTAC 6 cut(s) 178, 379, 430, 872, 883, 996
SaqAI TTAA 3 cut(s) 821, 908, 1020
SatI GCNGC 1 cut(s) 804
Sau3AI GATC 5 cut(s) 522, 573, 592, 759, 978
Sau96I GGNCC 2 cut(s) 393, 693
ScaI AGTACT 1 cut(s) 997
ScrFI CCNGG 2 cut(s) 387, 397
SduI GDGCHC 1 cut(s) 756
SfaNI GCATC 1 cut(s) 13
SfuI TTCGAA 1 cut(s) 222
SmlI CTYRAG 1 cut(s) 969
SmoI CTYRAG 1 cut(s) 969
Sse9I AATT 5 cut(s) 90, 116, 844, 898, 1021
SsiI CCGC 2 cut(s) 749, 803
SspMI CTAG 3 cut(s) 674, 720, 944
StyD4I CCNGG 2 cut(s) 385, 395
TaaI ACNGT 8 cut(s) 177, 182, 354, 612, 662, 876, 966, 1000
TaqII GACCGA 2 cut(s) 245, 752
TasI AATT 5 cut(s) 90, 116, 844, 898, 1021
TatI WGTACW 3 cut(s) 378, 871, 995
TauI GCSGC 1 cut(s) 806
TfiI GAWTC 5 cut(s) 203, 281, 301, 579, 740
Tru1I TTAA 3 cut(s) 821, 908, 1020
Tru9I TTAA 3 cut(s) 821, 908, 1020
TscAI CASTG 1 cut(s) 423
TseFI GTSAC 3 cut(s) 164, 294, 764
Tsp45I GTSAC 3 cut(s) 164, 294, 764
TspDTI ATGAA 2 cut(s) 526, 659
TspGWI ACGGA 3 cut(s) 603, 618, 900
TspRI CASTG 1 cut(s) 423
Tth111I GACNNNGTC 1 cut(s) 860
Van91I CCANNNNNTGG 1 cut(s) 317
VneI GTGCAC 1 cut(s) 752
XapI RAATTY 1 cut(s) 844
XbaI TCTAGA 1 cut(s) 673
XmiI GTMKAC 1 cut(s) 259
XspI CTAG 3 cut(s) 674, 720, 944
ZraI GACGTC 3 cut(s) 142, 412, 862
ZrmI AGTACT 1 cut(s) 997
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.