RLG00000028634

Zinc-dependent metalloprotease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0016658
Physical Location & Seq
Reverse (-)
25438240 .. 25439373
1134 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028634

Sequence Viewer

Length: 852 bp
ATGTTTCGGTTATTCAGTTTCTCTCTCTCCCTCTTTATCTTCTTCCTCAAATTCCCGCCATGCATACCCACCAGAACAGTCCCCAAAAACACCGGAATATCAGAGATAATTGCAGCACCGAAATGTGGTGGACCATCACCTGACATGAGCTCAAATACATTGCATGGCACGAAACATTACGTGTATTTCCCCGGAAAACCTCGGTGGGTCCCACGCACGATGCCCATGAGACTCACCTACGCTTTCTCGGCGGAGAACATGATCAGTTACTTGAGCTCGTCCGATATCCGAGACGCATTTAAACGCTCTTTTGATCGTTGGGCGTCGGTTATTCCGGTGAGCTTCTTCGAGACGGAAGACTACGGCTTCTCGGATATCAAAATAGGGTTTTACGCCGGGGACCACGGCGATGGAGAGCCTTTCGACGGTGTGCTTGGGATTTTAGCCCACTCGTTTTCTCCGGAGACTGGGAGGCTCCACCTTGATGCGGCGGAGACGTGGGCGGTGGACTTTAGGTTGGAGAAATCGGCGGTGGCTGTCGATTTAGAGTCCGTGGCGACTCATGAGATCGGGCATTTGCTAGGGTTAGGGCATAGTTTGGTGAAAGAGGCTGTGATGTACCCGAGTTTGAAGCCTAGGGAAAAGAAGGTTGATTTACAGGTTGATGATGTTGAAGGAGTGCAAGCTTTGTATGGATCAAACCCCAATTATACATTCAGTGCTTTATCAGAGTCAGAATACAGTACTTCAACAAATCTGGGCGTTGATTTCAGGAGTAGACCATCAAGATGGGAAAATTTCGTCTTTGTTTTGGTTATCTGTATCACTGGTGATCTTGCTTTGGCTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M10

Protein Analysis

284

Amino Acids

31.46

Weight (kDa)

5.62

Isoelectric Point (pI)

38.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M10 PF00413 77 - 232 4.1e-48 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016658)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45040
fragaria_vesca FvH4_7g10690
malus_domestica MD00G1063700.v1.1
prunus_persica Prupe.2G135100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0348631
rosa_laevigata RLG00000028634
rosa_multiflora Rmu_sc0004109.1_g000009
rosa_roxburghii Rroxscaffold_4G00306870
rosa_rugosa Rorug01G0195900
rosa_samantha Rh1AG213600 Rh1BG180100 Rh1CG198200 Rh1DG210000
rosa_wichuraiana Rw1G018090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 778
AccIII TCCGGA 1 cut(s) 460
AciI CCGC 6 cut(s) 56, 251, 488, 491, 503, 530
AclWI GGATC 1 cut(s) 703
AcsI RAATTY 2 cut(s) 50, 796
AcyI GRCGYC 1 cut(s) 323
AfaI GTAC 2 cut(s) 620, 745
AfiI CCNNNNNNNGG 4 cut(s) 125, 425, 467, 487
AflIII ACRYGT 1 cut(s) 180
AgsI TTSAA 3 cut(s) 631, 674, 750
AjiI CACGTC 1 cut(s) 498
AjuI GAANNNNNNNTTGG 2 cut(s) 698, 730
AluBI AGCT 4 cut(s) 150, 276, 342, 686
AluI AGCT 4 cut(s) 150, 276, 342, 686
Alw21I GWGCWC 2 cut(s) 152, 278
Alw26I GTCTC 5 cut(s) 223, 285, 344, 458, 488
AlwI GGATC 1 cut(s) 703
Ama87I CYCGRG 1 cut(s) 622
Aor13HI TCCGGA 1 cut(s) 460
ApeKI GCWGC 2 cut(s) 113, 845
ApoI RAATTY 2 cut(s) 50, 796
AspA2I CCTAGG 1 cut(s) 635
AspS9I GGNCC 3 cut(s) 131, 208, 400
AsuC2I CCSGG 2 cut(s) 192, 397
AsuHPI GGTGA 5 cut(s) 129, 226, 349, 613, 842
AvaI CYCGRG 1 cut(s) 622
AvaII GGWCC 3 cut(s) 131, 208, 400
AvrII CCTAGG 1 cut(s) 635
BanII GRGCYC 2 cut(s) 152, 278
BbsI GAAGAC 1 cut(s) 363
Bbv12I GWGCWC 2 cut(s) 152, 278
BbvI GCAGC 2 cut(s) 125, 832
BccI CCATC 4 cut(s) 142, 404, 783, 790
BceAI ACGGC 2 cut(s) 379, 421
BclI TGATCA 1 cut(s) 261
BcnI CCSGG 2 cut(s) 192, 397
BcoDI GTCTC 5 cut(s) 223, 285, 344, 458, 488
BfaI CTAG 2 cut(s) 581, 636
BisI GCNGC 3 cut(s) 114, 489, 846
BlnI CCTAGG 1 cut(s) 635
BlsI GCNGC 3 cut(s) 115, 490, 847
BmcAI AGTACT 1 cut(s) 745
Bme1390I CCNGG 2 cut(s) 192, 397
Bme18I GGWCC 3 cut(s) 131, 208, 400
BmeT110I CYCGRG 1 cut(s) 622
BmgBI CACGTC 1 cut(s) 498
BmgT120I GGNCC 3 cut(s) 131, 208, 400
BmiI GGNNCC 4 cut(s) 209, 210, 401, 476
BmrFI CCNGG 2 cut(s) 192, 397
BmrI ACTGGG 1 cut(s) 477
BmsI GCATC 2 cut(s) 210, 475
BmuI ACTGGG 1 cut(s) 477
BpiI GAAGAC 1 cut(s) 363
BpuEI CTTGAG 1 cut(s) 292
BpuMI CCSGG 2 cut(s) 192, 397
BsaAI YACGTR 1 cut(s) 181
BsaHI GRCGYC 1 cut(s) 323
BsaJI CCNNGG 6 cut(s) 190, 200, 396, 403, 552, 635
BsaWI WCCGGW 3 cut(s) 92, 334, 460
Bsc4I CCNNNNNNNGG 4 cut(s) 125, 425, 467, 487
Bse1I ACTGG 2 cut(s) 472, 832
Bse3DI GCAATG 1 cut(s) 158
BseAI TCCGGA 1 cut(s) 460
BseDI CCNNGG 6 cut(s) 190, 200, 396, 403, 552, 635
BseLI CCNNNNNNNGG 4 cut(s) 125, 425, 467, 487
BseMI GCAATG 1 cut(s) 158
BseNI ACTGG 2 cut(s) 472, 832
BseXI GCAGC 2 cut(s) 125, 832
BsiHKAI GWGCWC 2 cut(s) 152, 278
BsiHKCI CYCGRG 1 cut(s) 622
BsiSI CCGG 5 cut(s) 93, 192, 335, 396, 461
BslFI GGGAC 3 cut(s) 65, 194, 413
BslI CCNNNNNNNGG 4 cut(s) 125, 425, 467, 487
BsmAI GTCTC 5 cut(s) 223, 285, 344, 458, 488
BsmBI CGTCTC 3 cut(s) 285, 344, 488
BsmFI GGGAC 3 cut(s) 65, 194, 413
BsoBI CYCGRG 1 cut(s) 622
Bsp1286I GDGCHC 2 cut(s) 152, 278
Bsp13I TCCGGA 1 cut(s) 460
Bsp143I GATC 5 cut(s) 261, 313, 567, 695, 832
BspACI CCGC 6 cut(s) 56, 251, 488, 491, 503, 530
BspEI TCCGGA 1 cut(s) 460
BspHI TCATGA 1 cut(s) 562
BspLI GGNNCC 4 cut(s) 209, 210, 401, 476
BspPI GGATC 1 cut(s) 703
BsrDI GCAATG 1 cut(s) 158
BsrI ACTGG 2 cut(s) 472, 832
BssECI CCNNGG 6 cut(s) 190, 200, 396, 403, 552, 635
BssMI GATC 5 cut(s) 261, 313, 567, 695, 832
BssNI GRCGYC 1 cut(s) 323
BssT1I CCWWGG 1 cut(s) 635
Bst4CI ACNGT 3 cut(s) 79, 428, 743
BstACI GRCGYC 1 cut(s) 323
BstBAI YACGTR 1 cut(s) 181
BstC8I GCNNGC 1 cut(s) 684
BstDSI CCRYGG 2 cut(s) 403, 552
BstKTI GATC 5 cut(s) 264, 316, 570, 698, 835
BstMAI GTCTC 5 cut(s) 223, 285, 344, 458, 488
BstMBI GATC 5 cut(s) 261, 313, 567, 695, 832
BstMWI GCNNNNNNNGC 2 cut(s) 248, 845
BstSCI CCNGG 2 cut(s) 190, 395
BstV1I GCAGC 2 cut(s) 125, 832
BstV2I GAAGAC 1 cut(s) 363
BstXI CCANNNNNNTGG 2 cut(s) 410, 789
BtgI CCRYGG 2 cut(s) 403, 552
BtgZI GCGATG 1 cut(s) 423
BtrI CACGTC 1 cut(s) 498
BtsIMutI CAGTG 2 cut(s) 724, 825
Cac8I GCNNGC 1 cut(s) 684
CciI TCATGA 1 cut(s) 562
Cfr13I GGNCC 3 cut(s) 131, 208, 400
CseI GACGC 2 cut(s) 302, 312
Csp6I GTAC 2 cut(s) 619, 744
CviAII CATG 6 cut(s) 60, 145, 164, 226, 259, 563
CviQI GTAC 2 cut(s) 619, 744
DpnI GATC 5 cut(s) 263, 315, 569, 697, 834
DpnII GATC 5 cut(s) 261, 313, 567, 695, 832
DraI TTTAAA 1 cut(s) 301
EciI GGCGGA 2 cut(s) 266, 506
Ecl136II GAGCTC 2 cut(s) 150, 276
Eco130I CCWWGG 1 cut(s) 635
Eco24I GRGCYC 2 cut(s) 152, 278
Eco32I GATATC 2 cut(s) 286, 376
Eco47I GGWCC 3 cut(s) 131, 208, 400
Eco53kI GAGCTC 2 cut(s) 150, 276
Eco88I CYCGRG 1 cut(s) 622
EcoICRI GAGCTC 2 cut(s) 150, 276
EcoO109I RGGNCCY 1 cut(s) 208
EcoRV GATATC 2 cut(s) 286, 376
EcoT14I CCWWGG 1 cut(s) 635
EcoT22I ATGCAT 1 cut(s) 65
EcoT38I GRGCYC 2 cut(s) 152, 278
ErhI CCWWGG 1 cut(s) 635
Esp3I CGTCTC 3 cut(s) 285, 344, 488
FaeI CATG 6 cut(s) 63, 148, 167, 229, 262, 566
FaqI GGGAC 3 cut(s) 65, 194, 413
FatI CATG 6 cut(s) 59, 144, 163, 225, 258, 562
FauI CCCGC 1 cut(s) 63
FbaI TGATCA 1 cut(s) 261
FblI GTMKAC 1 cut(s) 778
Fnu4HI GCNGC 3 cut(s) 114, 489, 846
FriOI GRGCYC 2 cut(s) 152, 278
Fsp4HI GCNGC 3 cut(s) 114, 489, 846
FspBI CTAG 2 cut(s) 581, 636
GluI GCNGC 3 cut(s) 114, 489, 846
HapII CCGG 5 cut(s) 93, 192, 335, 396, 461
HgaI GACGC 2 cut(s) 302, 312
Hin1I GRCGYC 1 cut(s) 323
Hin1II CATG 6 cut(s) 63, 148, 167, 229, 262, 566
HindIII AAGCTT 1 cut(s) 684
HinfI GANTC 4 cut(s) 231, 548, 559, 731
HpaII CCGG 5 cut(s) 93, 192, 335, 396, 461
HphI GGTGA 5 cut(s) 129, 226, 349, 613, 842
Hpy166II GTNNAC 3 cut(s) 131, 508, 779
Hpy188I TCNGA 6 cut(s) 103, 283, 290, 373, 730, 736
Hpy188III TCNNGA 5 cut(s) 349, 461, 563, 772, 786
Hpy8I GTNNAC 3 cut(s) 131, 508, 779
Hpy99I CGWCG 2 cut(s) 328, 428
HpyAV CCTTC 2 cut(s) 640, 668
HpyCH4III ACNGT 3 cut(s) 79, 428, 743
HpyCH4IV ACGT 2 cut(s) 180, 497
HpyCH4V TGCA 5 cut(s) 63, 113, 163, 682, 848
HpyF10VI GCNNNNNNNGC 2 cut(s) 248, 845
HpySE526I ACGT 2 cut(s) 180, 497
Hsp92I GRCGYC 1 cut(s) 323
Hsp92II CATG 6 cut(s) 63, 148, 167, 229, 262, 566
KflI GGGWCCC 1 cut(s) 208
Kpn2I TCCGGA 1 cut(s) 460
Ksp22I TGATCA 1 cut(s) 261
Kzo9I GATC 5 cut(s) 261, 313, 567, 695, 832
LmnI GCTCC 1 cut(s) 480
Lsp1109I GCAGC 2 cut(s) 125, 832
LweI GCATC 2 cut(s) 210, 475
MaeI CTAG 2 cut(s) 581, 636
MaeII ACGT 2 cut(s) 180, 497
MaeIII GTNAC 1 cut(s) 266
MalI GATC 5 cut(s) 263, 315, 569, 697, 834
MboI GATC 5 cut(s) 261, 313, 567, 695, 832
MboII GAAGA 4 cut(s) 31, 34, 337, 368
MhlI GDGCHC 2 cut(s) 152, 278
MluCI AATT 4 cut(s) 50, 108, 706, 796
MlyI GAGTC 4 cut(s) 225, 553, 557, 740
MmeI TCCRAC 1 cut(s) 498
MnlI CCTC 5 cut(s) 41, 56, 210, 465, 601
Mph1103I ATGCAT 1 cut(s) 65
MroI TCCGGA 1 cut(s) 460
MseI TTAA 1 cut(s) 300
MslI CAYNNNNRTG 4 cut(s) 121, 408, 483, 787
MspI CCGG 5 cut(s) 93, 192, 335, 396, 461
MspR9I CCNGG 2 cut(s) 192, 397
MwoI GCNNNNNNNGC 2 cut(s) 248, 845
NciI CCSGG 2 cut(s) 192, 397
NdeII GATC 5 cut(s) 261, 313, 567, 695, 832
NlaIII CATG 6 cut(s) 63, 148, 167, 229, 262, 566
NlaIV GGNNCC 4 cut(s) 209, 210, 401, 476
NmeAIII GCCGAG 1 cut(s) 227
NsiI ATGCAT 1 cut(s) 65
PagI TCATGA 1 cut(s) 562
PkrI GCNGC 3 cut(s) 115, 490, 847
PleI GAGTC 4 cut(s) 225, 553, 556, 739
PpsI GAGTC 4 cut(s) 225, 553, 556, 739
Ppu21I YACGTR 1 cut(s) 181
PpuMI RGGWCCY 1 cut(s) 208
Psp124BI GAGCTC 2 cut(s) 152, 278
Psp5II RGGWCCY 1 cut(s) 208
PspN4I GGNNCC 4 cut(s) 209, 210, 401, 476
PspPI GGNCC 3 cut(s) 131, 208, 400
PspPPI RGGWCCY 1 cut(s) 208
RsaI GTAC 2 cut(s) 620, 745
RsaNI GTAC 2 cut(s) 619, 744
RseI CAYNNNNRTG 4 cut(s) 121, 408, 483, 787
SacI GAGCTC 2 cut(s) 152, 278
SaqAI TTAA 1 cut(s) 300
SatI GCNGC 3 cut(s) 114, 489, 846
Sau3AI GATC 5 cut(s) 261, 313, 567, 695, 832
Sau96I GGNCC 3 cut(s) 131, 208, 400
ScaI AGTACT 1 cut(s) 745
SchI GAGTC 4 cut(s) 225, 553, 557, 740
ScrFI CCNGG 2 cut(s) 192, 397
SduI GDGCHC 2 cut(s) 152, 278
SfaNI GCATC 2 cut(s) 210, 475
SinI GGWCC 3 cut(s) 131, 208, 400
SmiMI CAYNNNNRTG 4 cut(s) 121, 408, 483, 787
SmlI CTYRAG 1 cut(s) 271
SmoI CTYRAG 1 cut(s) 271
Sse9I AATT 4 cut(s) 50, 108, 706, 796
SsiI CCGC 6 cut(s) 56, 251, 488, 491, 503, 530
SspMI CTAG 2 cut(s) 581, 636
SstI GAGCTC 2 cut(s) 152, 278
StyD4I CCNGG 2 cut(s) 190, 395
StyI CCWWGG 1 cut(s) 635
TaaI ACNGT 3 cut(s) 79, 428, 743
TaiI ACGT 2 cut(s) 183, 500
TaqI TCGA 3 cut(s) 348, 423, 540
TasI AATT 4 cut(s) 50, 108, 706, 796
TatI WGTACW 1 cut(s) 743
TauI GCSGC 1 cut(s) 491
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TscAI CASTG 2 cut(s) 724, 832
TseI GCWGC 2 cut(s) 113, 845
TspGWI ACGGA 2 cut(s) 368, 541
TspRI CASTG 2 cut(s) 724, 832
VpaK11BI GGWCC 3 cut(s) 131, 208, 400
XapI RAATTY 2 cut(s) 50, 796
XmaJI CCTAGG 1 cut(s) 635
XmiI GTMKAC 1 cut(s) 778
XspI CTAG 2 cut(s) 581, 636
ZrmI AGTACT 1 cut(s) 745
Zsp2I ATGCAT 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.