Prupe.2G135100_v2.0.a1

Zinc-dependent metalloprotease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0016658
Physical Location & Seq
Forward (+)
19284331 .. 19286003
1673 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G135100.1

Sequence Viewer

Length: 813 bp
ATGTCTCTGTTATTCAGTTATTCCCTCTCCCTTTTCTTCTTCTTCTTCTTCTTCTTCCTCAACCTCCATGCTCTGAGGGCCAGCCCCGACACCGGAATCTTATCATCAGAGCATACAAATCATCACTTCACGTTGCATGGCACACGACGTTACGTCTACTTTACAGGAAAACCTAGGTGGGTGCGTCCCATGACGCCCATGACACTCACCTACGCTTTCTCTCCTGACAACATGATCAACTCCTTGAGCTTGTCCGATATACGACAGGTGTTCAAACGGGCTTTTGCCAGATGGGCATCCGTGATTCCGGTGAGCTTCGCCGAGTCCGAGGACTATGGCTTCGCGGACATCAGAATAGGGTTTTATAGTGGTGATCATGGGGACGGAGAGCCGTTTGATGGGGTGCTTGGGATTTTAGCCCACTCGTTTTCACCGGAGAGCGGGAGGCTCCACCTCGACGCAGCGGAGACGTGGGCGGTGGACTTTGGGATGGAGAAGTCCCCAGTGGCCGTGGACTTGGAGTCGGTGGCCACGCATGAGATTGGTCACTTGCTAGGGTTAGGGCATAGTTTGGTGAAAGAGGCTATAATGTACCCGAATTTAAAGCCTAGGGATAAAAAGGTTGATTTGCAGCGTGATGATATTGAAGGAGTGCAAGCTCTTTACGGATCTAATCCAAATTATACGGTTGGAGATTTATCGGAGTCATACATTTCGACAAATTTGGCCGTGGATTTCAGAACTTCTAGACAATCAAAATGGGCCAATTTCATCCTGGCTTTGATCATAATCTGTTTGTGCATATGTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M10

Protein Analysis

271

Amino Acids

30.4

Weight (kDa)

5.85

Isoelectric Point (pI)

32.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016658)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45040
fragaria_vesca FvH4_7g10690
malus_domestica MD00G1063700.v1.1
prunus_persica Prupe.2G135100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0348631
rosa_laevigata RLG00000028634
rosa_multiflora Rmu_sc0004109.1_g000009
rosa_roxburghii Rroxscaffold_4G00306870
rosa_rugosa Rorug01G0195900
rosa_samantha Rh1AG213600 Rh1BG180100 Rh1CG198200 Rh1DG210000
rosa_wichuraiana Rw1G018090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 441
AccI GTMKAC 1 cut(s) 156
AccII CGCG 1 cut(s) 344
AciI CCGC 4 cut(s) 344, 441, 464, 476
AclWI GGATC 1 cut(s) 676
AcoI YGGCCR 3 cut(s) 507, 528, 726
AcsI RAATTY 2 cut(s) 598, 721
AcyI GRCGYC 1 cut(s) 194
AfaI GTAC 1 cut(s) 593
AfiI CCNNNNNNNGG 3 cut(s) 92, 398, 440
AgsI TTSAA 2 cut(s) 274, 647
AhdI GACNNNNNGTC 2 cut(s) 152, 520
AjiI CACGTC 1 cut(s) 471
AjnI CCWGG 1 cut(s) 774
AluBI AGCT 3 cut(s) 249, 315, 659
AluI AGCT 3 cut(s) 249, 315, 659
Alw26I GTCTC 2 cut(s) 9, 461
AlwI GGATC 1 cut(s) 676
AoxI GGCC 5 cut(s) 78, 507, 528, 726, 762
ApeKI GCWGC 2 cut(s) 461, 631
ApoI RAATTY 2 cut(s) 598, 721
ArsI GACNNNNNNTTYG 2 cut(s) 323, 355
AspA2I CCTAGG 2 cut(s) 173, 608
AspS9I GGNCC 2 cut(s) 78, 762
AsuHPI GGTGA 5 cut(s) 199, 322, 383, 423, 586
AvrII CCTAGG 2 cut(s) 173, 608
BalI TGGCCA 1 cut(s) 530
BbvI GCAGC 2 cut(s) 473, 643
BccI CCATC 3 cut(s) 285, 392, 484
BceAI ACGGC 3 cut(s) 376, 494, 713
BciT130I CCWGG 1 cut(s) 776
BclI TGATCA 3 cut(s) 234, 373, 783
BcoDI GTCTC 2 cut(s) 9, 461
BfaI CTAG 4 cut(s) 174, 554, 609, 747
BglI GCCNNNNNGGC 1 cut(s) 293
BisI GCNGC 2 cut(s) 462, 632
BlnI CCTAGG 2 cut(s) 173, 608
BlsI GCNGC 2 cut(s) 463, 633
Bme1390I CCNGG 1 cut(s) 776
BmeRI GACNNNNNGTC 2 cut(s) 152, 520
BmgBI CACGTC 1 cut(s) 471
BmgT120I GGNCC 2 cut(s) 78, 762
BmiI GGNNCC 1 cut(s) 449
BmrFI CCNGG 1 cut(s) 776
BmrI ACTGGG 1 cut(s) 497
BmsI GCATC 1 cut(s) 305
BmuI ACTGGG 1 cut(s) 497
BpuEI CTTGAG 1 cut(s) 265
BsaBI GATNNNNATC 2 cut(s) 295, 788
BsaHI GRCGYC 1 cut(s) 194
BsaJI CCNNGG 5 cut(s) 173, 327, 510, 608, 729
BsaWI WCCGGW 3 cut(s) 92, 307, 433
Bsc4I CCNNNNNNNGG 3 cut(s) 92, 398, 440
Bse1I ACTGG 1 cut(s) 503
Bse8I GATNNNNATC 2 cut(s) 295, 788
BseBI CCWGG 1 cut(s) 776
BseDI CCNNGG 5 cut(s) 173, 327, 510, 608, 729
BseGI GGATG 3 cut(s) 296, 495, 771
BseJI GATNNNNATC 2 cut(s) 295, 788
BseLI CCNNNNNNNGG 3 cut(s) 92, 398, 440
BseMII CTCAG 1 cut(s) 65
BseNI ACTGG 1 cut(s) 503
BseXI GCAGC 2 cut(s) 473, 643
Bsh1236I CGCG 1 cut(s) 344
BshFI GGCC 5 cut(s) 80, 509, 530, 728, 764
BsiSI CCGG 3 cut(s) 93, 308, 434
BslFI GGGAC 3 cut(s) 171, 395, 484
BslI CCNNNNNNNGG 3 cut(s) 92, 398, 440
BsmAI GTCTC 2 cut(s) 9, 461
BsmBI CGTCTC 1 cut(s) 461
BsmFI GGGAC 3 cut(s) 171, 395, 484
BsnI GGCC 5 cut(s) 80, 509, 530, 728, 764
Bsp143I GATC 4 cut(s) 234, 373, 668, 783
BspACI CCGC 4 cut(s) 344, 441, 464, 476
BspANI GGCC 5 cut(s) 80, 509, 530, 728, 764
BspCNI CTCAG 1 cut(s) 66
BspFNI CGCG 1 cut(s) 344
BspLI GGNNCC 1 cut(s) 449
BspPI GGATC 1 cut(s) 676
BsrBI CCGCTC 1 cut(s) 441
BsrI ACTGG 1 cut(s) 503
BssECI CCNNGG 5 cut(s) 173, 327, 510, 608, 729
BssMI GATC 4 cut(s) 234, 373, 668, 783
BssNI GRCGYC 1 cut(s) 194
BssT1I CCWWGG 2 cut(s) 173, 608
Bst2UI CCWGG 1 cut(s) 776
Bst4CI ACNGT 1 cut(s) 688
BstACI GRCGYC 1 cut(s) 194
BstC8I GCNNGC 2 cut(s) 82, 657
BstDEI CTNAG 1 cut(s) 74
BstDSI CCRYGG 2 cut(s) 510, 729
BstF5I GGATG 3 cut(s) 296, 495, 771
BstFNI CGCG 1 cut(s) 344
BstKTI GATC 4 cut(s) 237, 376, 671, 786
BstMAI GTCTC 2 cut(s) 9, 461
BstMBI GATC 4 cut(s) 234, 373, 668, 783
BstMWI GCNNNNNNNGC 2 cut(s) 77, 293
BstNI CCWGG 1 cut(s) 776
BstSCI CCNGG 1 cut(s) 774
BstUI CGCG 1 cut(s) 344
BstV1I GCAGC 2 cut(s) 473, 643
BstX2I RGATCY 1 cut(s) 668
BstYI RGATCY 1 cut(s) 668
BsuRI GGCC 5 cut(s) 80, 509, 530, 728, 764
BtgI CCRYGG 2 cut(s) 510, 729
BtrI CACGTC 1 cut(s) 471
BtsCI GGATG 3 cut(s) 296, 495, 771
BtsIMutI CAGTG 1 cut(s) 510
Cac8I GCNNGC 2 cut(s) 82, 657
Cfr13I GGNCC 2 cut(s) 78, 762
CseI GACGC 3 cut(s) 173, 202, 467
Csp6I GTAC 1 cut(s) 592
CviAII CATG 7 cut(s) 68, 137, 190, 199, 232, 377, 536
CviQI GTAC 1 cut(s) 592
DdeI CTNAG 1 cut(s) 74
DpnI GATC 4 cut(s) 236, 375, 670, 785
DpnII GATC 4 cut(s) 234, 373, 668, 783
DraI TTTAAA 1 cut(s) 603
DriI GACNNNNNGTC 2 cut(s) 152, 520
EaeI YGGCCR 3 cut(s) 507, 528, 726
Eam1105I GACNNNNNGTC 2 cut(s) 152, 520
Eco130I CCWWGG 2 cut(s) 173, 608
EcoRII CCWGG 1 cut(s) 774
EcoT14I CCWWGG 2 cut(s) 173, 608
ErhI CCWWGG 2 cut(s) 173, 608
Esp3I CGTCTC 1 cut(s) 461
FaeI CATG 7 cut(s) 71, 140, 193, 202, 235, 380, 539
FaqI GGGAC 3 cut(s) 171, 395, 484
FatI CATG 7 cut(s) 67, 136, 189, 198, 231, 376, 535
FauI CCCGC 1 cut(s) 434
FauNDI CATATG 1 cut(s) 803
FbaI TGATCA 3 cut(s) 234, 373, 783
FblI GTMKAC 1 cut(s) 156
Fnu4HI GCNGC 2 cut(s) 462, 632
FokI GGATG 3 cut(s) 283, 502, 758
Fsp4HI GCNGC 2 cut(s) 462, 632
FspBI CTAG 4 cut(s) 174, 554, 609, 747
GluI GCNGC 2 cut(s) 462, 632
HaeIII GGCC 5 cut(s) 80, 509, 530, 728, 764
HapII CCGG 3 cut(s) 93, 308, 434
HgaI GACGC 3 cut(s) 173, 202, 467
Hin1I GRCGYC 1 cut(s) 194
Hin1II CATG 7 cut(s) 71, 140, 193, 202, 235, 380, 539
HinfI GANTC 5 cut(s) 96, 304, 323, 521, 704
HpaII CCGG 3 cut(s) 93, 308, 434
HphI GGTGA 5 cut(s) 199, 322, 383, 423, 586
Hpy166II GTNNAC 3 cut(s) 157, 481, 514
Hpy188I TCNGA 7 cut(s) 75, 109, 256, 328, 353, 703, 740
Hpy188III TCNNGA 2 cut(s) 224, 747
Hpy8I GTNNAC 3 cut(s) 157, 481, 514
Hpy99I CGWCG 2 cut(s) 150, 461
HpyAV CCTTC 1 cut(s) 641
HpyCH4III ACNGT 1 cut(s) 688
HpyCH4IV ACGT 4 cut(s) 131, 148, 153, 470
HpyCH4V TGCA 4 cut(s) 136, 631, 655, 801
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 293
HpyF3I CTNAG 1 cut(s) 74
HpySE526I ACGT 4 cut(s) 131, 148, 153, 470
Hsp92I GRCGYC 1 cut(s) 194
Hsp92II CATG 7 cut(s) 71, 140, 193, 202, 235, 380, 539
Ksp22I TGATCA 3 cut(s) 234, 373, 783
Kzo9I GATC 4 cut(s) 234, 373, 668, 783
LmnI GCTCC 1 cut(s) 453
Lsp1109I GCAGC 2 cut(s) 473, 643
LweI GCATC 1 cut(s) 305
MaeI CTAG 4 cut(s) 174, 554, 609, 747
MaeII ACGT 4 cut(s) 131, 148, 153, 470
MaeIII GTNAC 2 cut(s) 149, 545
MalI GATC 4 cut(s) 236, 375, 670, 785
MbiI CCGCTC 1 cut(s) 441
MboI GATC 4 cut(s) 234, 373, 668, 783
MboII GAAGA 7 cut(s) 28, 31, 34, 37, 40, 43, 46
MflI RGATCY 1 cut(s) 668
MlsI TGGCCA 1 cut(s) 530
MluCI AATT 5 cut(s) 598, 679, 721, 766, 808
MluNI TGGCCA 1 cut(s) 530
MlyI GAGTC 3 cut(s) 332, 530, 713
MmeI TCCRAC 1 cut(s) 670
MnlI CCTC 8 cut(s) 35, 68, 69, 74, 322, 438, 464, 574
Mox20I TGGCCA 1 cut(s) 530
MscI TGGCCA 1 cut(s) 530
MseI TTAA 1 cut(s) 602
Msp20I TGGCCA 1 cut(s) 530
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 3 cut(s) 93, 308, 434
MspR9I CCNGG 1 cut(s) 776
MvaI CCWGG 1 cut(s) 776
MvnI CGCG 1 cut(s) 344
MwoI GCNNNNNNNGC 2 cut(s) 77, 293
NdeI CATATG 1 cut(s) 803
NdeII GATC 4 cut(s) 234, 373, 668, 783
NlaIII CATG 7 cut(s) 71, 140, 193, 202, 235, 380, 539
NlaIV GGNNCC 1 cut(s) 449
NmeAIII GCCGAG 1 cut(s) 346
NmuCI GTSAC 1 cut(s) 545
PcsI WCGNNNNNNNCGW 1 cut(s) 324
PfeI GAWTC 2 cut(s) 96, 304
PkrI GCNGC 2 cut(s) 463, 633
PleI GAGTC 3 cut(s) 331, 529, 712
PpsI GAGTC 3 cut(s) 331, 529, 712
Psp6I CCWGG 1 cut(s) 774
PspGI CCWGG 1 cut(s) 774
PspN4I GGNNCC 1 cut(s) 449
PspPI GGNCC 2 cut(s) 78, 762
PsuI RGATCY 1 cut(s) 668
RsaI GTAC 1 cut(s) 593
RsaNI GTAC 1 cut(s) 592
SaqAI TTAA 1 cut(s) 602
SatI GCNGC 2 cut(s) 462, 632
Sau3AI GATC 4 cut(s) 234, 373, 668, 783
Sau96I GGNCC 2 cut(s) 78, 762
SchI GAGTC 3 cut(s) 332, 530, 713
ScrFI CCNGG 1 cut(s) 776
SfaNI GCATC 1 cut(s) 305
SmlI CTYRAG 1 cut(s) 244
SmoI CTYRAG 1 cut(s) 244
Sse9I AATT 5 cut(s) 598, 679, 721, 766, 808
SsiI CCGC 4 cut(s) 344, 441, 464, 476
SspMI CTAG 4 cut(s) 174, 554, 609, 747
StyD4I CCNGG 1 cut(s) 774
StyI CCWWGG 2 cut(s) 173, 608
TaaI ACNGT 1 cut(s) 688
TaiI ACGT 4 cut(s) 134, 151, 156, 473
TaqI TCGA 2 cut(s) 456, 716
TasI AATT 5 cut(s) 598, 679, 721, 766, 808
TfiI GAWTC 2 cut(s) 96, 304
Tru1I TTAA 1 cut(s) 602
Tru9I TTAA 1 cut(s) 602
TscAI CASTG 1 cut(s) 510
TseFI GTSAC 1 cut(s) 545
TseI GCWGC 2 cut(s) 461, 631
Tsp45I GTSAC 1 cut(s) 545
TspDTI ATGAA 1 cut(s) 760
TspGWI ACGGA 3 cut(s) 289, 399, 681
TspRI CASTG 1 cut(s) 510
XapI RAATTY 2 cut(s) 598, 721
XbaI TCTAGA 1 cut(s) 746
XcmI CCANNNNNNNNNTGG 1 cut(s) 772
XmaJI CCTAGG 2 cut(s) 173, 608
XmiI GTMKAC 1 cut(s) 156
XspI CTAG 4 cut(s) 174, 554, 609, 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.