Rw1G018090

Zinc-dependent metalloprotease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0016658
Physical Location & Seq
Forward (+)
39823134 .. 39824182
1049 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G018090.1

Sequence Viewer

Length: 819 bp
ATGTTTCGGTTATTCAGTTACTCTCTCTCCCTCTTTATCTTCTTCCTCAAATTACCGCCATGCATACCCACCGGAATATCAGAGATAATTGCAGCACCGAAATGTGGTGCACCACCACCTGACATGAGCTCAAATACATTGCATGGCACGAAATATTACGTGTATTTCCCCGGAAAACCTCGGTGGGTCCCACGCACGATGCCCATGAGACTCACCTACGCTTTCTCGGCGGAGAACATGATCAGTTACTTGAGCTCGTCCGATATCCGAGACGCATTTAAACGCTCTTTTGATCGTTGGGCGTCGGTTATTCCGGTGAGCTTCTTCGAGACGGAAGACTACGGCTTCTCGGATATCAAAATAGGGTTTTACGCCGGGGACCACGGCGACGGAGAGCCTTTCGACGGTGTGCTTGGGATTTTAGCCCACTCGTTTTCGCCGGAGACCGGGAGGCTCCACCTTGATGCGGCGGAGACGTGGGCAGTGGACTTTAGGTCGGAGAAATCGGCGGTGGCTGTCGATTTAGAGTCCGTGGCGATTCATGAGATCGGGCATTTGCTAGGGTTAGGGCATAGTTTGGTGAAAGAGGCTGTGATGTACCCGAGTTTGAAGCCTAGGGAAAAGAAGGTTGATTTACAGGTTGATGATGTTGAAGGAGTGCAAGCTTTATATGGATCGAACCCCAATTATACATTCAGTGCTTTATCAGAGTCAGAATACAGTACTTCAACAAATCTGGGCGTTGATTTCAGGAGTAGACCATCAAGATGGGAAAATTTCGTCTTTGTTTTGGTTATCTGGTCGTGCATTTACTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M10

Protein Analysis

272

Amino Acids

30.56

Weight (kDa)

5.38

Isoelectric Point (pI)

40.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M10 PF00413 70 - 225 1.2e-48 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016658)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45040
fragaria_vesca FvH4_7g10690
malus_domestica MD00G1063700.v1.1
prunus_persica Prupe.2G135100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0348631
rosa_laevigata RLG00000028634
rosa_multiflora Rmu_sc0004109.1_g000009
rosa_roxburghii Rroxscaffold_4G00306870
rosa_rugosa Rorug01G0195900
rosa_samantha Rh1AG213600 Rh1BG180100 Rh1CG198200 Rh1DG210000
rosa_wichuraiana Rw1G018090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 757
AciI CCGC 5 cut(s) 56, 230, 467, 470, 509
AclWI GGATC 1 cut(s) 682
AcsI RAATTY 1 cut(s) 775
AcyI GRCGYC 1 cut(s) 302
AfaI GTAC 2 cut(s) 599, 724
AfiI CCNNNNNNNGG 4 cut(s) 104, 404, 446, 466
AflIII ACRYGT 1 cut(s) 159
AgsI TTSAA 3 cut(s) 610, 653, 729
AhdI GACNNNNNGTC 1 cut(s) 493
AjiI CACGTC 1 cut(s) 477
AjuI GAANNNNNNNTTGG 2 cut(s) 677, 709
AluBI AGCT 4 cut(s) 129, 255, 321, 665
AluI AGCT 4 cut(s) 129, 255, 321, 665
Alw21I GWGCWC 3 cut(s) 112, 131, 257
Alw26I GTCTC 5 cut(s) 202, 264, 323, 437, 467
Alw44I GTGCAC 1 cut(s) 108
AlwI GGATC 1 cut(s) 682
Ama87I CYCGRG 1 cut(s) 601
ApaLI GTGCAC 1 cut(s) 108
ApeKI GCWGC 1 cut(s) 92
ApoI RAATTY 1 cut(s) 775
AspA2I CCTAGG 1 cut(s) 614
AspS9I GGNCC 2 cut(s) 187, 379
AsuC2I CCSGG 3 cut(s) 171, 376, 448
AsuHPI GGTGA 3 cut(s) 205, 328, 592
AvaI CYCGRG 1 cut(s) 601
AvaII GGWCC 2 cut(s) 187, 379
AvrII CCTAGG 1 cut(s) 614
BaeGI GKGCMC 1 cut(s) 112
BanII GRGCYC 2 cut(s) 131, 257
BbsI GAAGAC 1 cut(s) 342
Bbv12I GWGCWC 3 cut(s) 112, 131, 257
BbvI GCAGC 1 cut(s) 104
BccI CCATC 2 cut(s) 762, 769
BceAI ACGGC 2 cut(s) 358, 400
BclI TGATCA 1 cut(s) 240
BcnI CCSGG 3 cut(s) 171, 376, 448
BcoDI GTCTC 5 cut(s) 202, 264, 323, 437, 467
BfaI CTAG 2 cut(s) 560, 615
BisI GCNGC 2 cut(s) 93, 468
BlnI CCTAGG 1 cut(s) 614
BlsI GCNGC 2 cut(s) 94, 469
BmcAI AGTACT 1 cut(s) 724
Bme1390I CCNGG 3 cut(s) 171, 376, 448
Bme18I GGWCC 2 cut(s) 187, 379
BmeRI GACNNNNNGTC 1 cut(s) 493
BmeT110I CYCGRG 1 cut(s) 601
BmgBI CACGTC 1 cut(s) 477
BmgT120I GGNCC 2 cut(s) 187, 379
BmiI GGNNCC 4 cut(s) 188, 189, 380, 455
BmrFI CCNGG 3 cut(s) 171, 376, 448
BmsI GCATC 2 cut(s) 189, 454
BpiI GAAGAC 1 cut(s) 342
BpuEI CTTGAG 1 cut(s) 271
BpuMI CCSGG 3 cut(s) 171, 376, 448
BsaAI YACGTR 1 cut(s) 160
BsaHI GRCGYC 1 cut(s) 302
BsaI GGTCTC 1 cut(s) 437
BsaJI CCNNGG 6 cut(s) 169, 179, 375, 382, 531, 614
BsaWI WCCGGW 2 cut(s) 71, 313
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 4 cut(s) 104, 404, 446, 466
Bse3DI GCAATG 1 cut(s) 137
BseDI CCNNGG 6 cut(s) 169, 179, 375, 382, 531, 614
BseLI CCNNNNNNNGG 4 cut(s) 104, 404, 446, 466
BseMI GCAATG 1 cut(s) 137
BseSI GKGCMC 1 cut(s) 112
BseXI GCAGC 1 cut(s) 104
BsiHKAI GWGCWC 3 cut(s) 112, 131, 257
BsiHKCI CYCGRG 1 cut(s) 601
BsiSI CCGG 6 cut(s) 72, 171, 314, 375, 440, 447
BslFI GGGAC 2 cut(s) 173, 392
BslI CCNNNNNNNGG 4 cut(s) 104, 404, 446, 466
BsmAI GTCTC 5 cut(s) 202, 264, 323, 437, 467
BsmBI CGTCTC 3 cut(s) 264, 323, 467
BsmFI GGGAC 2 cut(s) 173, 392
Bso31I GGTCTC 1 cut(s) 437
BsoBI CYCGRG 1 cut(s) 601
Bsp1286I GDGCHC 3 cut(s) 112, 131, 257
Bsp143I GATC 4 cut(s) 240, 292, 546, 674
BspACI CCGC 5 cut(s) 56, 230, 467, 470, 509
BspHI TCATGA 1 cut(s) 541
BspLI GGNNCC 4 cut(s) 188, 189, 380, 455
BspPI GGATC 1 cut(s) 682
BspTNI GGTCTC 1 cut(s) 437
BsrDI GCAATG 1 cut(s) 137
BssECI CCNNGG 6 cut(s) 169, 179, 375, 382, 531, 614
BssMI GATC 4 cut(s) 240, 292, 546, 674
BssNI GRCGYC 1 cut(s) 302
BssT1I CCWWGG 1 cut(s) 614
Bst4CI ACNGT 2 cut(s) 407, 722
BstACI GRCGYC 1 cut(s) 302
BstBAI YACGTR 1 cut(s) 160
BstC8I GCNNGC 1 cut(s) 663
BstDSI CCRYGG 2 cut(s) 382, 531
BstKTI GATC 4 cut(s) 243, 295, 549, 677
BstMAI GTCTC 5 cut(s) 202, 264, 323, 437, 467
BstMBI GATC 4 cut(s) 240, 292, 546, 674
BstMWI GCNNNNNNNGC 1 cut(s) 227
BstSCI CCNGG 3 cut(s) 169, 374, 446
BstSLI GKGCMC 1 cut(s) 112
BstV1I GCAGC 1 cut(s) 104
BstV2I GAAGAC 1 cut(s) 342
BstXI CCANNNNNNTGG 1 cut(s) 768
BtgI CCRYGG 2 cut(s) 382, 531
BtrI CACGTC 1 cut(s) 477
BtsI GCAGTG 1 cut(s) 489
BtsIMutI CAGTG 2 cut(s) 489, 703
Cac8I GCNNGC 1 cut(s) 663
CciI TCATGA 1 cut(s) 541
Cfr13I GGNCC 2 cut(s) 187, 379
CseI GACGC 2 cut(s) 281, 291
Csp6I GTAC 2 cut(s) 598, 723
CviAII CATG 6 cut(s) 60, 124, 143, 205, 238, 542
CviQI GTAC 2 cut(s) 598, 723
DpnI GATC 4 cut(s) 242, 294, 548, 676
DpnII GATC 4 cut(s) 240, 292, 546, 674
DraI TTTAAA 1 cut(s) 280
DriI GACNNNNNGTC 1 cut(s) 493
Eam1105I GACNNNNNGTC 1 cut(s) 493
EciI GGCGGA 2 cut(s) 245, 485
Ecl136II GAGCTC 2 cut(s) 129, 255
Eco130I CCWWGG 1 cut(s) 614
Eco24I GRGCYC 2 cut(s) 131, 257
Eco31I GGTCTC 1 cut(s) 437
Eco32I GATATC 2 cut(s) 265, 355
Eco47I GGWCC 2 cut(s) 187, 379
Eco53kI GAGCTC 2 cut(s) 129, 255
Eco88I CYCGRG 1 cut(s) 601
EcoICRI GAGCTC 2 cut(s) 129, 255
EcoO109I RGGNCCY 1 cut(s) 187
EcoRV GATATC 2 cut(s) 265, 355
EcoT14I CCWWGG 1 cut(s) 614
EcoT22I ATGCAT 1 cut(s) 65
EcoT38I GRGCYC 2 cut(s) 131, 257
ErhI CCWWGG 1 cut(s) 614
Esp3I CGTCTC 3 cut(s) 264, 323, 467
FaeI CATG 6 cut(s) 63, 127, 146, 208, 241, 545
FaqI GGGAC 2 cut(s) 173, 392
FatI CATG 6 cut(s) 59, 123, 142, 204, 237, 541
FbaI TGATCA 1 cut(s) 240
FblI GTMKAC 1 cut(s) 757
Fnu4HI GCNGC 2 cut(s) 93, 468
FriOI GRGCYC 2 cut(s) 131, 257
Fsp4HI GCNGC 2 cut(s) 93, 468
FspBI CTAG 2 cut(s) 560, 615
GluI GCNGC 2 cut(s) 93, 468
HapII CCGG 6 cut(s) 72, 171, 314, 375, 440, 447
HgaI GACGC 2 cut(s) 281, 291
Hin1I GRCGYC 1 cut(s) 302
Hin1II CATG 6 cut(s) 63, 127, 146, 208, 241, 545
HindIII AAGCTT 1 cut(s) 663
HinfI GANTC 4 cut(s) 210, 527, 538, 710
HpaII CCGG 6 cut(s) 72, 171, 314, 375, 440, 447
HphI GGTGA 3 cut(s) 205, 328, 592
Hpy166II GTNNAC 3 cut(s) 110, 487, 758
Hpy188I TCNGA 7 cut(s) 82, 262, 269, 352, 499, 709, 715
Hpy188III TCNNGA 4 cut(s) 328, 542, 751, 765
Hpy8I GTNNAC 3 cut(s) 110, 487, 758
Hpy99I CGWCG 3 cut(s) 307, 392, 407
HpyAV CCTTC 2 cut(s) 619, 647
HpyCH4III ACNGT 2 cut(s) 407, 722
HpyCH4IV ACGT 2 cut(s) 159, 476
HpyCH4V TGCA 6 cut(s) 63, 92, 110, 142, 661, 807
HpyF10VI GCNNNNNNNGC 1 cut(s) 227
HpySE526I ACGT 2 cut(s) 159, 476
Hsp92I GRCGYC 1 cut(s) 302
Hsp92II CATG 6 cut(s) 63, 127, 146, 208, 241, 545
KflI GGGWCCC 1 cut(s) 187
Ksp22I TGATCA 1 cut(s) 240
Kzo9I GATC 4 cut(s) 240, 292, 546, 674
LmnI GCTCC 1 cut(s) 459
Lsp1109I GCAGC 1 cut(s) 104
LweI GCATC 2 cut(s) 189, 454
MaeI CTAG 2 cut(s) 560, 615
MaeII ACGT 2 cut(s) 159, 476
MaeIII GTNAC 2 cut(s) 17, 245
MalI GATC 4 cut(s) 242, 294, 548, 676
MboI GATC 4 cut(s) 240, 292, 546, 674
MboII GAAGA 4 cut(s) 31, 34, 316, 347
MhlI GDGCHC 3 cut(s) 112, 131, 257
MluCI AATT 4 cut(s) 50, 87, 685, 775
MlyI GAGTC 3 cut(s) 204, 536, 719
MmeI TCCRAC 1 cut(s) 477
MnlI CCTC 5 cut(s) 41, 56, 189, 444, 580
Mph1103I ATGCAT 1 cut(s) 65
MseI TTAA 1 cut(s) 279
MslI CAYNNNNRTG 3 cut(s) 100, 462, 766
MspI CCGG 6 cut(s) 72, 171, 314, 375, 440, 447
MspR9I CCNGG 3 cut(s) 171, 376, 448
MwoI GCNNNNNNNGC 1 cut(s) 227
NciI CCSGG 3 cut(s) 171, 376, 448
NdeII GATC 4 cut(s) 240, 292, 546, 674
NlaIII CATG 6 cut(s) 63, 127, 146, 208, 241, 545
NlaIV GGNNCC 4 cut(s) 188, 189, 380, 455
NmeAIII GCCGAG 1 cut(s) 206
NsiI ATGCAT 1 cut(s) 65
PagI TCATGA 1 cut(s) 541
PfeI GAWTC 1 cut(s) 538
PkrI GCNGC 2 cut(s) 94, 469
PleI GAGTC 3 cut(s) 204, 535, 718
PpsI GAGTC 3 cut(s) 204, 535, 718
Ppu21I YACGTR 1 cut(s) 160
PpuMI RGGWCCY 1 cut(s) 187
Psp124BI GAGCTC 2 cut(s) 131, 257
Psp5II RGGWCCY 1 cut(s) 187
PspN4I GGNNCC 4 cut(s) 188, 189, 380, 455
PspPI GGNCC 2 cut(s) 187, 379
PspPPI RGGWCCY 1 cut(s) 187
RsaI GTAC 2 cut(s) 599, 724
RsaNI GTAC 2 cut(s) 598, 723
RseI CAYNNNNRTG 3 cut(s) 100, 462, 766
SacI GAGCTC 2 cut(s) 131, 257
SaqAI TTAA 1 cut(s) 279
SatI GCNGC 2 cut(s) 93, 468
Sau3AI GATC 4 cut(s) 240, 292, 546, 674
Sau96I GGNCC 2 cut(s) 187, 379
ScaI AGTACT 1 cut(s) 724
SchI GAGTC 3 cut(s) 204, 536, 719
ScrFI CCNGG 3 cut(s) 171, 376, 448
SduI GDGCHC 3 cut(s) 112, 131, 257
SfaNI GCATC 2 cut(s) 189, 454
SinI GGWCC 2 cut(s) 187, 379
SmiMI CAYNNNNRTG 3 cut(s) 100, 462, 766
SmlI CTYRAG 1 cut(s) 250
SmoI CTYRAG 1 cut(s) 250
Sse9I AATT 4 cut(s) 50, 87, 685, 775
SsiI CCGC 5 cut(s) 56, 230, 467, 470, 509
SspI AATATT 1 cut(s) 155
SspMI CTAG 2 cut(s) 560, 615
SstI GAGCTC 2 cut(s) 131, 257
StyD4I CCNGG 3 cut(s) 169, 374, 446
StyI CCWWGG 1 cut(s) 614
TaaI ACNGT 2 cut(s) 407, 722
TaiI ACGT 2 cut(s) 162, 479
TaqI TCGA 4 cut(s) 327, 402, 519, 677
TasI AATT 4 cut(s) 50, 87, 685, 775
TatI WGTACW 1 cut(s) 722
TauI GCSGC 1 cut(s) 470
TfiI GAWTC 1 cut(s) 538
Tru1I TTAA 1 cut(s) 279
Tru9I TTAA 1 cut(s) 279
TscAI CASTG 2 cut(s) 489, 703
TseI GCWGC 1 cut(s) 92
TspDTI ATGAA 1 cut(s) 530
TspGWI ACGGA 3 cut(s) 347, 405, 520
TspRI CASTG 2 cut(s) 489, 703
VneI GTGCAC 1 cut(s) 108
VpaK11BI GGWCC 2 cut(s) 187, 379
XapI RAATTY 1 cut(s) 775
XmaJI CCTAGG 1 cut(s) 614
XmiI GTMKAC 1 cut(s) 757
XspI CTAG 2 cut(s) 560, 615
ZrmI AGTACT 1 cut(s) 724
Zsp2I ATGCAT 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.