Rh1BG180100

Zinc-dependent metalloprotease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0016658
Physical Location & Seq
Forward (+)
28756964 .. 28758484
1521 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG180100.1

Sequence Viewer

Length: 840 bp
ATGTTTCGGTTATTCAGTTACTCTCTCTCCCTCTTTATCTTCTTCCTCAAATTCCCGCCATGCATACCCACCAGAACAGTTCCCAAAAACACCGGAATATCAGAGATAATTGCAGCACCGAAATGTGGTGCACCACCACCTGACATGAGCTCAAATACATTGCATGGCACGAAACATTACGTGTATTTCCCCGGAAAACCTCGGTGGGTCCCACGCACGATGCCCATGAGACTCACCTACGCTTTCTCGGCGGAGAACATGATCAGTTATTTGAGCTCGTCCGATATCCGAGACGCATTTAAACGCTCTTTTGATCGTTGGGCGTCGGTTATTCCGGTGAGCTTCTTCGAGACGGAAGACTACGGCTTCTCGGATATCAAAATAGGGTTTTACGCCGGGGACCACGGCGACGGAGAGCCTTTCGACGGTGTGCTTGGGATTTTAGCCCACTCATTTTCGCCGGAGACCGGGAGGCTCCACCTTGATGCTGCGGAGACGTGGGCGGTGGACTTTAGGTCGGAGAAATCGGCGGTGGCTGTCGATTTAGAGTCCGTGGCGACTCATGAGATCGGGCATTTGCTAGGGTTAGGGCATAGTTTGGTGAAAGAGGCTGTGATGTACCCGAGTTTGAAGCCTAGGGAAAAGAAGGTTGATTTACAGGTTGATGATGTTGAAGGAGTGCAAGCTTTGTATGGATCGAACCCCAATTATACATTCAGTGCTTTATCTGAGTCAGAATACAGTACTTCAACAAATCTGGGCGTTGATTTCAGGAGTAGACCATCAAGATGGGAAAATTTCGTCTTTGTTTTGGTTATCTGGTTGTGCATTTACTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M10

Protein Analysis

279

Amino Acids

31.37

Weight (kDa)

5.76

Isoelectric Point (pI)

40.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M10 PF00413 77 - 232 1.7e-48 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016658)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45040
fragaria_vesca FvH4_7g10690
malus_domestica MD00G1063700.v1.1
prunus_persica Prupe.2G135100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0348631
rosa_laevigata RLG00000028634
rosa_multiflora Rmu_sc0004109.1_g000009
rosa_roxburghii Rroxscaffold_4G00306870
rosa_rugosa Rorug01G0195900
rosa_samantha Rh1AG213600 Rh1BG180100 Rh1CG198200 Rh1DG210000
rosa_wichuraiana Rw1G018090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 778
AciI CCGC 5 cut(s) 56, 251, 491, 503, 530
AclWI GGATC 1 cut(s) 703
AcsI RAATTY 2 cut(s) 50, 796
AcyI GRCGYC 1 cut(s) 323
AfaI GTAC 2 cut(s) 620, 745
AfiI CCNNNNNNNGG 3 cut(s) 125, 425, 467
AflIII ACRYGT 1 cut(s) 180
AgsI TTSAA 3 cut(s) 631, 674, 750
AhdI GACNNNNNGTC 1 cut(s) 514
AjiI CACGTC 1 cut(s) 498
AjuI GAANNNNNNNTTGG 2 cut(s) 698, 730
AluBI AGCT 4 cut(s) 150, 276, 342, 686
AluI AGCT 4 cut(s) 150, 276, 342, 686
Alw21I GWGCWC 3 cut(s) 133, 152, 278
Alw26I GTCTC 5 cut(s) 223, 285, 344, 458, 488
Alw44I GTGCAC 1 cut(s) 129
AlwI GGATC 1 cut(s) 703
Ama87I CYCGRG 1 cut(s) 622
ApaLI GTGCAC 1 cut(s) 129
ApeKI GCWGC 2 cut(s) 113, 488
ApoI RAATTY 2 cut(s) 50, 796
AspA2I CCTAGG 1 cut(s) 635
AspS9I GGNCC 2 cut(s) 208, 400
AsuC2I CCSGG 3 cut(s) 192, 397, 469
AsuHPI GGTGA 3 cut(s) 226, 349, 613
AvaI CYCGRG 1 cut(s) 622
AvaII GGWCC 2 cut(s) 208, 400
AvrII CCTAGG 1 cut(s) 635
BaeGI GKGCMC 1 cut(s) 133
BanII GRGCYC 2 cut(s) 152, 278
BbsI GAAGAC 1 cut(s) 363
Bbv12I GWGCWC 3 cut(s) 133, 152, 278
BbvI GCAGC 2 cut(s) 125, 475
BccI CCATC 2 cut(s) 783, 790
BceAI ACGGC 2 cut(s) 379, 421
BclI TGATCA 1 cut(s) 261
BcnI CCSGG 3 cut(s) 192, 397, 469
BcoDI GTCTC 5 cut(s) 223, 285, 344, 458, 488
BfaI CTAG 2 cut(s) 581, 636
BisI GCNGC 2 cut(s) 114, 489
BlnI CCTAGG 1 cut(s) 635
BlsI GCNGC 2 cut(s) 115, 490
BmcAI AGTACT 1 cut(s) 745
Bme1390I CCNGG 3 cut(s) 192, 397, 469
Bme18I GGWCC 2 cut(s) 208, 400
BmeRI GACNNNNNGTC 1 cut(s) 514
BmeT110I CYCGRG 1 cut(s) 622
BmgBI CACGTC 1 cut(s) 498
BmgT120I GGNCC 2 cut(s) 208, 400
BmiI GGNNCC 4 cut(s) 209, 210, 401, 476
BmrFI CCNGG 3 cut(s) 192, 397, 469
BmsI GCATC 2 cut(s) 210, 475
BpiI GAAGAC 1 cut(s) 363
BpuMI CCSGG 3 cut(s) 192, 397, 469
BsaAI YACGTR 1 cut(s) 181
BsaHI GRCGYC 1 cut(s) 323
BsaI GGTCTC 1 cut(s) 458
BsaJI CCNNGG 6 cut(s) 190, 200, 396, 403, 552, 635
BsaWI WCCGGW 2 cut(s) 92, 334
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 3 cut(s) 125, 425, 467
Bse3DI GCAATG 1 cut(s) 158
BseDI CCNNGG 6 cut(s) 190, 200, 396, 403, 552, 635
BseLI CCNNNNNNNGG 3 cut(s) 125, 425, 467
BseMI GCAATG 1 cut(s) 158
BseMII CTCAG 1 cut(s) 720
BseSI GKGCMC 1 cut(s) 133
BseXI GCAGC 2 cut(s) 125, 475
BsiHKAI GWGCWC 3 cut(s) 133, 152, 278
BsiHKCI CYCGRG 1 cut(s) 622
BsiSI CCGG 6 cut(s) 93, 192, 335, 396, 461, 468
BslFI GGGAC 2 cut(s) 194, 413
BslI CCNNNNNNNGG 3 cut(s) 125, 425, 467
BsmAI GTCTC 5 cut(s) 223, 285, 344, 458, 488
BsmBI CGTCTC 3 cut(s) 285, 344, 488
BsmFI GGGAC 2 cut(s) 194, 413
Bso31I GGTCTC 1 cut(s) 458
BsoBI CYCGRG 1 cut(s) 622
Bsp1286I GDGCHC 3 cut(s) 133, 152, 278
Bsp143I GATC 4 cut(s) 261, 313, 567, 695
BspACI CCGC 5 cut(s) 56, 251, 491, 503, 530
BspCNI CTCAG 1 cut(s) 721
BspHI TCATGA 1 cut(s) 562
BspLI GGNNCC 4 cut(s) 209, 210, 401, 476
BspPI GGATC 1 cut(s) 703
BspTNI GGTCTC 1 cut(s) 458
BsrDI GCAATG 1 cut(s) 158
BssECI CCNNGG 6 cut(s) 190, 200, 396, 403, 552, 635
BssMI GATC 4 cut(s) 261, 313, 567, 695
BssNI GRCGYC 1 cut(s) 323
BssT1I CCWWGG 1 cut(s) 635
Bst4CI ACNGT 3 cut(s) 79, 428, 743
BstACI GRCGYC 1 cut(s) 323
BstBAI YACGTR 1 cut(s) 181
BstC8I GCNNGC 1 cut(s) 684
BstDEI CTNAG 1 cut(s) 729
BstDSI CCRYGG 2 cut(s) 403, 552
BstKTI GATC 4 cut(s) 264, 316, 570, 698
BstMAI GTCTC 5 cut(s) 223, 285, 344, 458, 488
BstMBI GATC 4 cut(s) 261, 313, 567, 695
BstMWI GCNNNNNNNGC 1 cut(s) 248
BstSCI CCNGG 3 cut(s) 190, 395, 467
BstSLI GKGCMC 1 cut(s) 133
BstV1I GCAGC 2 cut(s) 125, 475
BstV2I GAAGAC 1 cut(s) 363
BstXI CCANNNNNNTGG 1 cut(s) 789
BtgI CCRYGG 2 cut(s) 403, 552
BtrI CACGTC 1 cut(s) 498
BtsIMutI CAGTG 1 cut(s) 724
Cac8I GCNNGC 1 cut(s) 684
CciI TCATGA 1 cut(s) 562
Cfr13I GGNCC 2 cut(s) 208, 400
CseI GACGC 2 cut(s) 302, 312
Csp6I GTAC 2 cut(s) 619, 744
CviAII CATG 6 cut(s) 60, 145, 164, 226, 259, 563
CviQI GTAC 2 cut(s) 619, 744
DdeI CTNAG 1 cut(s) 729
DpnI GATC 4 cut(s) 263, 315, 569, 697
DpnII GATC 4 cut(s) 261, 313, 567, 695
DraI TTTAAA 1 cut(s) 301
DriI GACNNNNNGTC 1 cut(s) 514
Eam1105I GACNNNNNGTC 1 cut(s) 514
EciI GGCGGA 1 cut(s) 266
Ecl136II GAGCTC 2 cut(s) 150, 276
Eco130I CCWWGG 1 cut(s) 635
Eco24I GRGCYC 2 cut(s) 152, 278
Eco31I GGTCTC 1 cut(s) 458
Eco32I GATATC 2 cut(s) 286, 376
Eco47I GGWCC 2 cut(s) 208, 400
Eco53kI GAGCTC 2 cut(s) 150, 276
Eco88I CYCGRG 1 cut(s) 622
EcoICRI GAGCTC 2 cut(s) 150, 276
EcoO109I RGGNCCY 1 cut(s) 208
EcoRV GATATC 2 cut(s) 286, 376
EcoT14I CCWWGG 1 cut(s) 635
EcoT22I ATGCAT 1 cut(s) 65
EcoT38I GRGCYC 2 cut(s) 152, 278
ErhI CCWWGG 1 cut(s) 635
Esp3I CGTCTC 3 cut(s) 285, 344, 488
FaeI CATG 6 cut(s) 63, 148, 167, 229, 262, 566
FaqI GGGAC 2 cut(s) 194, 413
FatI CATG 6 cut(s) 59, 144, 163, 225, 258, 562
FauI CCCGC 1 cut(s) 63
FbaI TGATCA 1 cut(s) 261
FblI GTMKAC 1 cut(s) 778
Fnu4HI GCNGC 2 cut(s) 114, 489
FriOI GRGCYC 2 cut(s) 152, 278
Fsp4HI GCNGC 2 cut(s) 114, 489
FspBI CTAG 2 cut(s) 581, 636
GluI GCNGC 2 cut(s) 114, 489
HapII CCGG 6 cut(s) 93, 192, 335, 396, 461, 468
HgaI GACGC 2 cut(s) 302, 312
Hin1I GRCGYC 1 cut(s) 323
Hin1II CATG 6 cut(s) 63, 148, 167, 229, 262, 566
HindIII AAGCTT 1 cut(s) 684
HinfI GANTC 4 cut(s) 231, 548, 559, 731
HpaII CCGG 6 cut(s) 93, 192, 335, 396, 461, 468
HphI GGTGA 3 cut(s) 226, 349, 613
Hpy166II GTNNAC 3 cut(s) 131, 508, 779
Hpy188I TCNGA 7 cut(s) 103, 283, 290, 373, 520, 730, 736
Hpy188III TCNNGA 4 cut(s) 349, 563, 772, 786
Hpy8I GTNNAC 3 cut(s) 131, 508, 779
Hpy99I CGWCG 3 cut(s) 328, 413, 428
HpyAV CCTTC 2 cut(s) 640, 668
HpyCH4III ACNGT 3 cut(s) 79, 428, 743
HpyCH4IV ACGT 2 cut(s) 180, 497
HpyCH4V TGCA 6 cut(s) 63, 113, 131, 163, 682, 828
HpyF10VI GCNNNNNNNGC 1 cut(s) 248
HpyF3I CTNAG 1 cut(s) 729
HpySE526I ACGT 2 cut(s) 180, 497
Hsp92I GRCGYC 1 cut(s) 323
Hsp92II CATG 6 cut(s) 63, 148, 167, 229, 262, 566
KflI GGGWCCC 1 cut(s) 208
Ksp22I TGATCA 1 cut(s) 261
Kzo9I GATC 4 cut(s) 261, 313, 567, 695
LmnI GCTCC 1 cut(s) 480
Lsp1109I GCAGC 2 cut(s) 125, 475
LweI GCATC 2 cut(s) 210, 475
MaeI CTAG 2 cut(s) 581, 636
MaeII ACGT 2 cut(s) 180, 497
MaeIII GTNAC 1 cut(s) 17
MalI GATC 4 cut(s) 263, 315, 569, 697
MboI GATC 4 cut(s) 261, 313, 567, 695
MboII GAAGA 4 cut(s) 31, 34, 337, 368
MhlI GDGCHC 3 cut(s) 133, 152, 278
MluCI AATT 4 cut(s) 50, 108, 706, 796
MlyI GAGTC 4 cut(s) 225, 553, 557, 740
MmeI TCCRAC 1 cut(s) 498
MnlI CCTC 5 cut(s) 41, 56, 210, 465, 601
Mph1103I ATGCAT 1 cut(s) 65
MseI TTAA 1 cut(s) 300
MslI CAYNNNNRTG 3 cut(s) 121, 483, 787
MspI CCGG 6 cut(s) 93, 192, 335, 396, 461, 468
MspR9I CCNGG 3 cut(s) 192, 397, 469
MwoI GCNNNNNNNGC 1 cut(s) 248
NciI CCSGG 3 cut(s) 192, 397, 469
NdeII GATC 4 cut(s) 261, 313, 567, 695
NlaIII CATG 6 cut(s) 63, 148, 167, 229, 262, 566
NlaIV GGNNCC 4 cut(s) 209, 210, 401, 476
NmeAIII GCCGAG 1 cut(s) 227
NsiI ATGCAT 1 cut(s) 65
PagI TCATGA 1 cut(s) 562
PkrI GCNGC 2 cut(s) 115, 490
PleI GAGTC 4 cut(s) 225, 553, 556, 739
PpsI GAGTC 4 cut(s) 225, 553, 556, 739
Ppu21I YACGTR 1 cut(s) 181
PpuMI RGGWCCY 1 cut(s) 208
Psp124BI GAGCTC 2 cut(s) 152, 278
Psp5II RGGWCCY 1 cut(s) 208
PspN4I GGNNCC 4 cut(s) 209, 210, 401, 476
PspPI GGNCC 2 cut(s) 208, 400
PspPPI RGGWCCY 1 cut(s) 208
RsaI GTAC 2 cut(s) 620, 745
RsaNI GTAC 2 cut(s) 619, 744
RseI CAYNNNNRTG 3 cut(s) 121, 483, 787
SacI GAGCTC 2 cut(s) 152, 278
SaqAI TTAA 1 cut(s) 300
SatI GCNGC 2 cut(s) 114, 489
Sau3AI GATC 4 cut(s) 261, 313, 567, 695
Sau96I GGNCC 2 cut(s) 208, 400
ScaI AGTACT 1 cut(s) 745
SchI GAGTC 4 cut(s) 225, 553, 557, 740
ScrFI CCNGG 3 cut(s) 192, 397, 469
SduI GDGCHC 3 cut(s) 133, 152, 278
SfaNI GCATC 2 cut(s) 210, 475
SinI GGWCC 2 cut(s) 208, 400
SmiMI CAYNNNNRTG 3 cut(s) 121, 483, 787
Sse9I AATT 4 cut(s) 50, 108, 706, 796
SsiI CCGC 5 cut(s) 56, 251, 491, 503, 530
SspMI CTAG 2 cut(s) 581, 636
SstI GAGCTC 2 cut(s) 152, 278
StyD4I CCNGG 3 cut(s) 190, 395, 467
StyI CCWWGG 1 cut(s) 635
TaaI ACNGT 3 cut(s) 79, 428, 743
TaiI ACGT 2 cut(s) 183, 500
TaqI TCGA 4 cut(s) 348, 423, 540, 698
TasI AATT 4 cut(s) 50, 108, 706, 796
TatI WGTACW 1 cut(s) 743
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TscAI CASTG 1 cut(s) 724
TseI GCWGC 2 cut(s) 113, 488
TspGWI ACGGA 3 cut(s) 368, 426, 541
TspRI CASTG 1 cut(s) 724
VneI GTGCAC 1 cut(s) 129
VpaK11BI GGWCC 2 cut(s) 208, 400
XapI RAATTY 2 cut(s) 50, 796
XmaJI CCTAGG 1 cut(s) 635
XmiI GTMKAC 1 cut(s) 778
XspI CTAG 2 cut(s) 581, 636
ZrmI AGTACT 1 cut(s) 745
Zsp2I ATGCAT 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.