Rroxscaffold_4G00306870

Zinc-dependent metalloprotease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0016658
Physical Location & Seq
Reverse (-)
27832335 .. 27832949
615 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00306870.1

Sequence Viewer

Length: 615 bp
ATGCCCATGAGACTCACCTACGCTTTCTCGGCGGAGAACATGATCAGTTACTTGAGCTCGTCCGATATCCGAGACGCATTTAAACGCTCTTTTGATCGTTGGGCGTCGGTTATTCCGGTGAGCTTCTTCGAGACGGAAGACTACGGCTTCTCTGATATCAAAATAGGGTTTTACGCCGGGGACCACGGCGACGGAGAGCCTTTCGACGGTGTGCTTGGGATTTTAGCCCACTCGTTTTCGCCGGAGACCGGGAGGCTCCACCTTGATGCGGCGGAGACGTGGGCGGTGGACTTTAGGTCGGAGAAATCGGCAGTAGCTGTCGATTTAGAGTCCGTGGCGACTCATGAGATCGGGCATTTGCTAGGGTTAGGGCATAGTTTGGTGAAAGAGGCTGTGATGTACCCGAGTTTGAAGCCTAGGGAAAAGAAGGTTGATTTACAGGTTGATGATGTTGAAGGAGTGCAAGCTTTGTATGGATCGAACCCCAATTATACATTCAGTGCTTTATCAGAGTCAGAATACAGTACTTCAACAAATCTGGGCGTTGATTTCAGGAGTAGACCATCAAGATGGGAAAATTTCGTCTTTGTTTTGGTTATCTGGTTATGCATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M10

Protein Analysis

204

Amino Acids

22.81

Weight (kDa)

4.77

Isoelectric Point (pI)

39.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M10 PF00413 4 - 159 1.2e-48 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016658)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45040
fragaria_vesca FvH4_7g10690
malus_domestica MD00G1063700.v1.1
prunus_persica Prupe.2G135100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0348631
rosa_laevigata RLG00000028634
rosa_multiflora Rmu_sc0004109.1_g000009
rosa_roxburghii Rroxscaffold_4G00306870
rosa_rugosa Rorug01G0195900
rosa_samantha Rh1AG213600 Rh1BG180100 Rh1CG198200 Rh1DG210000
rosa_wichuraiana Rw1G018090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 559
AciI CCGC 4 cut(s) 32, 269, 272, 284
AclWI GGATC 1 cut(s) 484
AcsI RAATTY 1 cut(s) 577
AcyI GRCGYC 1 cut(s) 104
AfaI GTAC 2 cut(s) 401, 526
AfiI CCNNNNNNNGG 3 cut(s) 206, 248, 268
AgsI TTSAA 3 cut(s) 412, 455, 531
AhdI GACNNNNNGTC 1 cut(s) 295
AjiI CACGTC 1 cut(s) 279
AjuI GAANNNNNNNTTGG 2 cut(s) 479, 511
AluBI AGCT 4 cut(s) 57, 123, 317, 467
AluI AGCT 4 cut(s) 57, 123, 317, 467
Alw21I GWGCWC 1 cut(s) 59
Alw26I GTCTC 5 cut(s) 4, 66, 125, 239, 269
AlwI GGATC 1 cut(s) 484
AlwNI CAGNNNCTG 1 cut(s) 317
Ama87I CYCGRG 1 cut(s) 403
ApoI RAATTY 1 cut(s) 577
AspA2I CCTAGG 1 cut(s) 416
AspS9I GGNCC 1 cut(s) 181
AsuC2I CCSGG 2 cut(s) 178, 250
AsuHPI GGTGA 3 cut(s) 7, 130, 394
AvaI CYCGRG 1 cut(s) 403
AvaII GGWCC 1 cut(s) 181
AvrII CCTAGG 1 cut(s) 416
BanII GRGCYC 1 cut(s) 59
BbsI GAAGAC 1 cut(s) 144
Bbv12I GWGCWC 1 cut(s) 59
BccI CCATC 2 cut(s) 564, 571
BceAI ACGGC 2 cut(s) 160, 202
BclI TGATCA 1 cut(s) 42
BcnI CCSGG 2 cut(s) 178, 250
BcoDI GTCTC 5 cut(s) 4, 66, 125, 239, 269
BfaI CTAG 2 cut(s) 362, 417
BisI GCNGC 1 cut(s) 270
BlnI CCTAGG 1 cut(s) 416
BlsI GCNGC 1 cut(s) 271
BmcAI AGTACT 1 cut(s) 526
Bme1390I CCNGG 2 cut(s) 178, 250
Bme18I GGWCC 1 cut(s) 181
BmeRI GACNNNNNGTC 1 cut(s) 295
BmeT110I CYCGRG 1 cut(s) 403
BmgBI CACGTC 1 cut(s) 279
BmgT120I GGNCC 1 cut(s) 181
BmiI GGNNCC 2 cut(s) 182, 257
BmrFI CCNGG 2 cut(s) 178, 250
BmsI GCATC 1 cut(s) 256
BpiI GAAGAC 1 cut(s) 144
BpuEI CTTGAG 1 cut(s) 73
BpuMI CCSGG 2 cut(s) 178, 250
BsaHI GRCGYC 1 cut(s) 104
BsaI GGTCTC 1 cut(s) 239
BsaJI CCNNGG 4 cut(s) 177, 184, 333, 416
BsaWI WCCGGW 1 cut(s) 115
Bsc4I CCNNNNNNNGG 3 cut(s) 206, 248, 268
BseDI CCNNGG 4 cut(s) 177, 184, 333, 416
BseLI CCNNNNNNNGG 3 cut(s) 206, 248, 268
BsiHKAI GWGCWC 1 cut(s) 59
BsiHKCI CYCGRG 1 cut(s) 403
BsiSI CCGG 4 cut(s) 116, 177, 242, 249
BslFI GGGAC 1 cut(s) 194
BslI CCNNNNNNNGG 3 cut(s) 206, 248, 268
BsmAI GTCTC 5 cut(s) 4, 66, 125, 239, 269
BsmBI CGTCTC 3 cut(s) 66, 125, 269
BsmFI GGGAC 1 cut(s) 194
Bso31I GGTCTC 1 cut(s) 239
BsoBI CYCGRG 1 cut(s) 403
Bsp1286I GDGCHC 1 cut(s) 59
Bsp143I GATC 4 cut(s) 42, 94, 348, 476
BspACI CCGC 4 cut(s) 32, 269, 272, 284
BspHI TCATGA 1 cut(s) 343
BspLI GGNNCC 2 cut(s) 182, 257
BspPI GGATC 1 cut(s) 484
BspTNI GGTCTC 1 cut(s) 239
BssECI CCNNGG 4 cut(s) 177, 184, 333, 416
BssMI GATC 4 cut(s) 42, 94, 348, 476
BssNI GRCGYC 1 cut(s) 104
BssT1I CCWWGG 1 cut(s) 416
Bst4CI ACNGT 2 cut(s) 209, 524
BstACI GRCGYC 1 cut(s) 104
BstC8I GCNNGC 1 cut(s) 465
BstDSI CCRYGG 2 cut(s) 184, 333
BstKTI GATC 4 cut(s) 45, 97, 351, 479
BstMAI GTCTC 5 cut(s) 4, 66, 125, 239, 269
BstMBI GATC 4 cut(s) 42, 94, 348, 476
BstMWI GCNNNNNNNGC 1 cut(s) 29
BstSCI CCNGG 2 cut(s) 176, 248
BstV2I GAAGAC 1 cut(s) 144
BstXI CCANNNNNNTGG 1 cut(s) 570
BtgI CCRYGG 2 cut(s) 184, 333
BtrI CACGTC 1 cut(s) 279
BtsIMutI CAGTG 1 cut(s) 505
Cac8I GCNNGC 1 cut(s) 465
CaiI CAGNNNCTG 1 cut(s) 317
CciI TCATGA 1 cut(s) 343
Cfr13I GGNCC 1 cut(s) 181
CseI GACGC 2 cut(s) 83, 93
Csp6I GTAC 2 cut(s) 400, 525
CviAII CATG 3 cut(s) 7, 40, 344
CviQI GTAC 2 cut(s) 400, 525
DpnI GATC 4 cut(s) 44, 96, 350, 478
DpnII GATC 4 cut(s) 42, 94, 348, 476
DraI TTTAAA 1 cut(s) 82
DriI GACNNNNNGTC 1 cut(s) 295
Eam1105I GACNNNNNGTC 1 cut(s) 295
EciI GGCGGA 2 cut(s) 47, 287
Ecl136II GAGCTC 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 416
Eco24I GRGCYC 1 cut(s) 59
Eco31I GGTCTC 1 cut(s) 239
Eco32I GATATC 2 cut(s) 67, 157
Eco47I GGWCC 1 cut(s) 181
Eco53kI GAGCTC 1 cut(s) 57
Eco88I CYCGRG 1 cut(s) 403
EcoICRI GAGCTC 1 cut(s) 57
EcoRV GATATC 2 cut(s) 67, 157
EcoT14I CCWWGG 1 cut(s) 416
EcoT22I ATGCAT 1 cut(s) 611
EcoT38I GRGCYC 1 cut(s) 59
ErhI CCWWGG 1 cut(s) 416
Esp3I CGTCTC 3 cut(s) 66, 125, 269
FaeI CATG 3 cut(s) 10, 43, 347
FaiI YATR 7 cut(s) 8, 41, 345, 375, 474, 492, 607
FaqI GGGAC 1 cut(s) 194
FatI CATG 3 cut(s) 6, 39, 343
FbaI TGATCA 1 cut(s) 42
FblI GTMKAC 1 cut(s) 559
Fnu4HI GCNGC 1 cut(s) 270
FriOI GRGCYC 1 cut(s) 59
Fsp4HI GCNGC 1 cut(s) 270
FspBI CTAG 2 cut(s) 362, 417
GluI GCNGC 1 cut(s) 270
HapII CCGG 4 cut(s) 116, 177, 242, 249
HgaI GACGC 2 cut(s) 83, 93
Hin1I GRCGYC 1 cut(s) 104
Hin1II CATG 3 cut(s) 10, 43, 347
HindIII AAGCTT 1 cut(s) 465
HinfI GANTC 4 cut(s) 12, 329, 340, 512
HpaII CCGG 4 cut(s) 116, 177, 242, 249
HphI GGTGA 3 cut(s) 7, 130, 394
Hpy166II GTNNAC 2 cut(s) 289, 560
Hpy188I TCNGA 6 cut(s) 64, 71, 154, 301, 511, 517
Hpy188III TCNNGA 4 cut(s) 130, 344, 553, 567
Hpy8I GTNNAC 2 cut(s) 289, 560
Hpy99I CGWCG 3 cut(s) 109, 194, 209
HpyAV CCTTC 2 cut(s) 421, 449
HpyCH4III ACNGT 2 cut(s) 209, 524
HpyCH4IV ACGT 1 cut(s) 278
HpyCH4V TGCA 2 cut(s) 463, 609
HpyF10VI GCNNNNNNNGC 1 cut(s) 29
HpySE526I ACGT 1 cut(s) 278
Hsp92I GRCGYC 1 cut(s) 104
Hsp92II CATG 3 cut(s) 10, 43, 347
Ksp22I TGATCA 1 cut(s) 42
Kzo9I GATC 4 cut(s) 42, 94, 348, 476
LmnI GCTCC 1 cut(s) 261
LpnPI CCDG 8 cut(s) 129, 190, 255, 262, 425, 524, 538, 586
LweI GCATC 1 cut(s) 256
MaeI CTAG 2 cut(s) 362, 417
MaeII ACGT 1 cut(s) 278
MaeIII GTNAC 1 cut(s) 47
MalI GATC 4 cut(s) 44, 96, 350, 478
MboI GATC 4 cut(s) 42, 94, 348, 476
MboII GAAGA 2 cut(s) 118, 149
MhlI GDGCHC 1 cut(s) 59
MluCI AATT 2 cut(s) 487, 577
MlyI GAGTC 4 cut(s) 6, 334, 338, 521
MmeI TCCRAC 1 cut(s) 279
MnlI CCTC 2 cut(s) 246, 382
Mph1103I ATGCAT 1 cut(s) 611
MseI TTAA 2 cut(s) 81, 613
MslI CAYNNNNRTG 2 cut(s) 264, 568
MspI CCGG 4 cut(s) 116, 177, 242, 249
MspR9I CCNGG 2 cut(s) 178, 250
MwoI GCNNNNNNNGC 1 cut(s) 29
NciI CCSGG 2 cut(s) 178, 250
NdeII GATC 4 cut(s) 42, 94, 348, 476
NlaIII CATG 3 cut(s) 10, 43, 347
NlaIV GGNNCC 2 cut(s) 182, 257
NmeAIII GCCGAG 1 cut(s) 8
NsiI ATGCAT 1 cut(s) 611
PagI TCATGA 1 cut(s) 343
PkrI GCNGC 1 cut(s) 271
PleI GAGTC 4 cut(s) 6, 334, 337, 520
PpsI GAGTC 4 cut(s) 6, 334, 337, 520
Psp124BI GAGCTC 1 cut(s) 59
PspN4I GGNNCC 2 cut(s) 182, 257
PspPI GGNCC 1 cut(s) 181
PstNI CAGNNNCTG 1 cut(s) 317
RsaI GTAC 2 cut(s) 401, 526
RsaNI GTAC 2 cut(s) 400, 525
RseI CAYNNNNRTG 2 cut(s) 264, 568
SacI GAGCTC 1 cut(s) 59
SaqAI TTAA 2 cut(s) 81, 613
SatI GCNGC 1 cut(s) 270
Sau3AI GATC 4 cut(s) 42, 94, 348, 476
Sau96I GGNCC 1 cut(s) 181
ScaI AGTACT 1 cut(s) 526
SchI GAGTC 4 cut(s) 6, 334, 338, 521
ScrFI CCNGG 2 cut(s) 178, 250
SduI GDGCHC 1 cut(s) 59
SfaNI GCATC 1 cut(s) 256
SinI GGWCC 1 cut(s) 181
SmiMI CAYNNNNRTG 2 cut(s) 264, 568
SmlI CTYRAG 1 cut(s) 52
SmoI CTYRAG 1 cut(s) 52
Sse9I AATT 2 cut(s) 487, 577
SsiI CCGC 4 cut(s) 32, 269, 272, 284
SspMI CTAG 2 cut(s) 362, 417
SstI GAGCTC 1 cut(s) 59
StyD4I CCNGG 2 cut(s) 176, 248
StyI CCWWGG 1 cut(s) 416
TaaI ACNGT 2 cut(s) 209, 524
TaiI ACGT 1 cut(s) 281
TaqI TCGA 4 cut(s) 129, 204, 321, 479
TasI AATT 2 cut(s) 487, 577
TatI WGTACW 1 cut(s) 524
TauI GCSGC 1 cut(s) 272
Tru1I TTAA 2 cut(s) 81, 613
Tru9I TTAA 2 cut(s) 81, 613
TscAI CASTG 1 cut(s) 505
TspGWI ACGGA 3 cut(s) 149, 207, 322
TspRI CASTG 1 cut(s) 505
VpaK11BI GGWCC 1 cut(s) 181
XapI RAATTY 1 cut(s) 577
XmaJI CCTAGG 1 cut(s) 416
XmiI GTMKAC 1 cut(s) 559
XspI CTAG 2 cut(s) 362, 417
ZrmI AGTACT 1 cut(s) 526
Zsp2I ATGCAT 1 cut(s) 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.