MD00G1063700.v1.1

Zinc-dependent metalloprotease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0016658
Physical Location & Seq
Forward (+)
11821945 .. 11822820
876 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1063700.v1.1.491

Sequence Viewer

Length: 876 bp
ATGTCTCTGTTATTCAGTTATTGTCTCTACCTCTCCTTCTTCTTCTTGTTGCTGCTAAACGTAACGCCATCCTTTTCCACTAGAACAGTGCCAACGATAGCCTCCGACACATTGAAGAAAATCGAGGCATTCTTCTATGCTCGGAGCGACAGCTACGCTGCCGCCAGAAAATCAGAGTATATACACCATAGTTTCACGTTGCATGGCATACAACATTACGTGTATTTTCCTGAAAAACCTAGGTGGGTGCGTATGAAGCCCATGACACTCACCTACGCAATCTCTAATGACAACCTGATTAACTACTTGAGCTTATCCGATATACAGCATGTGTTTAAACGTGCTTTCTCCAGATGGGCATCCGTGATTCCGGTGAGTTTCATGGAGACTAATGACTACGGCTTTGCCGACATCAAAATAGGGTTTTATAGCGGTGATCATGGGGATGGAGAGCCTTTTGATGGGGTGCTAGGGATTTTGGCCCACTCGTTTTCGCCTGTGAGCGGAAGGCTCCACCTTGATGCAGCGGAGAGGTGGGCAGTGGACTTTCGGAAGGAGAAGTCCATGGTGGCCGTGGATTTGGAGTCGGTGGCGGTGCATGAGATTGGACATTTACTAGGGTTGGGGCATAGTTTGGTGAAAGAGGCTGTTATGTATCCGAGTTTGAAGCCTCGGGACAGGAAGGTCGATTTGCATCTTGATGACATTGAAGGTGTTCAAGCTTTGTATGGATCAAACCCAAATTATACGGTTAGCGATTTATCGGAGTCGTACATTTCAACAAACGTGGCCGTTGATTTCAAAAGCAATAGACCATCAACATGGGGAAATATCATCTTGGTTTTGATTATGATATTTCTATGCATGTATTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M10

Protein Analysis

292

Amino Acids

33.09

Weight (kDa)

6.3

Isoelectric Point (pI)

35.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M10 PF00413 86 - 244 4.2e-50 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016658)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45040
fragaria_vesca FvH4_7g10690
malus_domestica MD00G1063700.v1.1
prunus_persica Prupe.2G135100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0348631
rosa_laevigata RLG00000028634
rosa_multiflora Rmu_sc0004109.1_g000009
rosa_roxburghii Rroxscaffold_4G00306870
rosa_rugosa Rorug01G0195900
rosa_samantha Rh1AG213600 Rh1BG180100 Rh1CG198200 Rh1DG210000
rosa_wichuraiana Rw1G018090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 683
AccBSI CCGCTC 1 cut(s) 504
AciI CCGC 5 cut(s) 162, 432, 504, 527, 593
AclWI GGATC 1 cut(s) 739
AcoI YGGCCR 2 cut(s) 570, 789
AfaI GTAC 1 cut(s) 773
AfiI CCNNNNNNNGG 2 cut(s) 461, 503
AflIII ACRYGT 1 cut(s) 219
AgsI TTSAA 6 cut(s) 115, 667, 710, 719, 780, 802
AluBI AGCT 3 cut(s) 153, 312, 722
AluI AGCT 3 cut(s) 153, 312, 722
Alw26I GTCTC 3 cut(s) 9, 29, 380
AlwI GGATC 1 cut(s) 739
Ama87I CYCGRG 1 cut(s) 672
AoxI GGCC 3 cut(s) 480, 570, 789
ApeKI GCWGC 3 cut(s) 52, 158, 524
ArsI GACNNNNNNTTYG 2 cut(s) 386, 418
Asp700I GAANNNNTTC 1 cut(s) 714
AspA2I CCTAGG 1 cut(s) 239
AspS9I GGNCC 1 cut(s) 481
AsuHPI GGTGA 4 cut(s) 262, 385, 446, 649
AvaI CYCGRG 1 cut(s) 672
AvrII CCTAGG 1 cut(s) 239
BbvI GCAGC 3 cut(s) 39, 145, 536
BccI CCATC 5 cut(s) 76, 348, 440, 455, 823
BceAI ACGGC 3 cut(s) 415, 557, 776
BciVI GTATCC 1 cut(s) 666
BclI TGATCA 1 cut(s) 436
BcoDI GTCTC 3 cut(s) 9, 29, 380
BfaI CTAG 4 cut(s) 81, 240, 470, 617
BfuI GTATCC 1 cut(s) 666
BisI GCNGC 4 cut(s) 53, 159, 162, 525
BlnI CCTAGG 1 cut(s) 239
BlsI GCNGC 4 cut(s) 54, 160, 163, 526
BmeT110I CYCGRG 1 cut(s) 672
BmgT120I GGNCC 1 cut(s) 481
BmiI GGNNCC 1 cut(s) 512
BmsI GCATC 3 cut(s) 368, 511, 703
BpmI CTGGAG 1 cut(s) 334
BpuEI CTTGAG 1 cut(s) 328
BsaAI YACGTR 1 cut(s) 220
BsaBI GATNNNNATC 2 cut(s) 358, 693
BsaJI CCNNGG 4 cut(s) 239, 564, 573, 671
BsaWI WCCGGW 1 cut(s) 370
Bsc4I CCNNNNNNNGG 2 cut(s) 461, 503
Bse8I GATNNNNATC 2 cut(s) 358, 693
BseDI CCNNGG 4 cut(s) 239, 564, 573, 671
BseGI GGATG 3 cut(s) 68, 359, 451
BseJI GATNNNNATC 2 cut(s) 358, 693
BseLI CCNNNNNNNGG 2 cut(s) 461, 503
BseXI GCAGC 3 cut(s) 39, 145, 536
BshFI GGCC 3 cut(s) 482, 572, 791
BsiHKCI CYCGRG 1 cut(s) 672
BsiSI CCGG 1 cut(s) 371
BslFI GGGAC 1 cut(s) 689
BslI CCNNNNNNNGG 2 cut(s) 461, 503
BsmAI GTCTC 3 cut(s) 9, 29, 380
BsmFI GGGAC 1 cut(s) 689
BsmI GAATGC 1 cut(s) 128
BsnI GGCC 3 cut(s) 482, 572, 791
BsoBI CYCGRG 1 cut(s) 672
Bsp143I GATC 2 cut(s) 436, 731
Bsp19I CCATGG 1 cut(s) 564
BspACI CCGC 5 cut(s) 162, 432, 504, 527, 593
BspANI GGCC 3 cut(s) 482, 572, 791
BspLI GGNNCC 1 cut(s) 512
BspPI GGATC 1 cut(s) 739
BsrBI CCGCTC 1 cut(s) 504
BssECI CCNNGG 4 cut(s) 239, 564, 573, 671
BssMI GATC 2 cut(s) 436, 731
BssT1I CCWWGG 2 cut(s) 239, 564
Bst4CI ACNGT 2 cut(s) 88, 751
BstBAI YACGTR 1 cut(s) 220
BstDSI CCRYGG 2 cut(s) 564, 573
BstF5I GGATG 3 cut(s) 68, 359, 451
BstKTI GATC 2 cut(s) 439, 734
BstMAI GTCTC 3 cut(s) 9, 29, 380
BstMBI GATC 2 cut(s) 436, 731
BstMWI GCNNNNNNNGC 1 cut(s) 256
BstNSI RCATGY 2 cut(s) 332, 868
BstV1I GCAGC 3 cut(s) 39, 145, 536
BstXI CCANNNNNNTGG 1 cut(s) 822
BsuI GTATCC 1 cut(s) 666
BsuRI GGCC 3 cut(s) 482, 572, 791
BtgI CCRYGG 2 cut(s) 564, 573
BtsCI GGATG 3 cut(s) 68, 359, 451
BtsI GCAGTG 1 cut(s) 546
BtsIMutI CAGTG 2 cut(s) 93, 546
Cfr13I GGNCC 1 cut(s) 481
Csp6I GTAC 1 cut(s) 772
CspCI CAANNNNNGTGG 2 cut(s) 768, 803
CviAII CATG 9 cut(s) 203, 262, 329, 382, 440, 565, 599, 822, 865
CviQI GTAC 1 cut(s) 772
DpnI GATC 2 cut(s) 438, 733
DpnII GATC 2 cut(s) 436, 731
DraI TTTAAA 1 cut(s) 337
DrdI GACNNNNNNGTC 1 cut(s) 683
DseDI GACNNNNNNGTC 1 cut(s) 683
EaeI YGGCCR 2 cut(s) 570, 789
Eco130I CCWWGG 2 cut(s) 239, 564
Eco88I CYCGRG 1 cut(s) 672
EcoT14I CCWWGG 2 cut(s) 239, 564
EcoT22I ATGCAT 1 cut(s) 866
ErhI CCWWGG 2 cut(s) 239, 564
FaeI CATG 9 cut(s) 206, 265, 332, 385, 443, 568, 602, 825, 868
FaqI GGGAC 1 cut(s) 689
FatI CATG 9 cut(s) 202, 261, 328, 381, 439, 564, 598, 821, 864
FbaI TGATCA 1 cut(s) 436
Fnu4HI GCNGC 4 cut(s) 53, 159, 162, 525
FokI GGATG 3 cut(s) 55, 346, 458
Fsp4HI GCNGC 4 cut(s) 53, 159, 162, 525
FspBI CTAG 4 cut(s) 81, 240, 470, 617
GluI GCNGC 4 cut(s) 53, 159, 162, 525
GsuI CTGGAG 1 cut(s) 334
HaeIII GGCC 3 cut(s) 482, 572, 791
HapII CCGG 1 cut(s) 371
Hin1II CATG 9 cut(s) 206, 265, 332, 385, 443, 568, 602, 825, 868
HindIII AAGCTT 1 cut(s) 720
HinfI GANTC 3 cut(s) 367, 584, 767
HpaII CCGG 1 cut(s) 371
HphI GGTGA 4 cut(s) 262, 385, 446, 649
Hpy166II GTNNAC 1 cut(s) 544
Hpy188I TCNGA 7 cut(s) 106, 144, 175, 319, 552, 660, 766
Hpy188III TCNNGA 4 cut(s) 230, 351, 674, 698
Hpy8I GTNNAC 1 cut(s) 544
HpyAV CCTTC 5 cut(s) 46, 501, 547, 676, 704
HpyCH4III ACNGT 2 cut(s) 88, 751
HpyCH4IV ACGT 5 cut(s) 60, 197, 219, 340, 786
HpyCH4V TGCA 5 cut(s) 202, 524, 598, 694, 864
HpyF10VI GCNNNNNNNGC 1 cut(s) 256
HpySE526I ACGT 5 cut(s) 60, 197, 219, 340, 786
Hsp92II CATG 9 cut(s) 206, 265, 332, 385, 443, 568, 602, 825, 868
Ksp22I TGATCA 1 cut(s) 436
Kzo9I GATC 2 cut(s) 436, 731
LmnI GCTCC 2 cut(s) 144, 516
LpnPI CCDG 7 cut(s) 178, 243, 308, 364, 384, 510, 664
Lsp1109I GCAGC 3 cut(s) 39, 145, 536
LweI GCATC 3 cut(s) 368, 511, 703
MaeI CTAG 4 cut(s) 81, 240, 470, 617
MaeII ACGT 5 cut(s) 60, 197, 219, 340, 786
MaeIII GTNAC 1 cut(s) 61
MalI GATC 2 cut(s) 438, 733
MbiI CCGCTC 1 cut(s) 504
MboI GATC 2 cut(s) 436, 731
MboII GAAGA 4 cut(s) 31, 34, 124, 127
MluCI AATT 1 cut(s) 742
MlyI GAGTC 2 cut(s) 593, 776
MmeI TCCRAC 1 cut(s) 129
MnlI CCTC 6 cut(s) 41, 112, 118, 525, 637, 681
Mph1103I ATGCAT 1 cut(s) 866
MroXI GAANNNNTTC 1 cut(s) 714
MseI TTAA 2 cut(s) 300, 336
MslI CAYNNNNRTG 4 cut(s) 444, 519, 699, 820
MspA1I CMGCKG 1 cut(s) 527
MspI CCGG 1 cut(s) 371
MssI GTTTAAAC 1 cut(s) 337
Mva1269I GAATGC 1 cut(s) 128
MwoI GCNNNNNNNGC 1 cut(s) 256
NcoI CCATGG 1 cut(s) 564
NdeII GATC 2 cut(s) 436, 731
NlaIII CATG 9 cut(s) 206, 265, 332, 385, 443, 568, 602, 825, 868
NlaIV GGNNCC 1 cut(s) 512
NsiI ATGCAT 1 cut(s) 866
NspI RCATGY 2 cut(s) 332, 868
PcsI WCGNNNNNNNCGW 1 cut(s) 405
PctI GAATGC 1 cut(s) 128
PdmI GAANNNNTTC 1 cut(s) 714
PfeI GAWTC 1 cut(s) 367
PkrI GCNGC 4 cut(s) 54, 160, 163, 526
PleI GAGTC 2 cut(s) 592, 775
PmeI GTTTAAAC 1 cut(s) 337
PpsI GAGTC 2 cut(s) 592, 775
Ppu21I YACGTR 1 cut(s) 220
PspN4I GGNNCC 1 cut(s) 512
PspPI GGNCC 1 cut(s) 481
RsaI GTAC 1 cut(s) 773
RsaNI GTAC 1 cut(s) 772
RseI CAYNNNNRTG 4 cut(s) 444, 519, 699, 820
SaqAI TTAA 2 cut(s) 300, 336
SatI GCNGC 4 cut(s) 53, 159, 162, 525
Sau3AI GATC 2 cut(s) 436, 731
Sau96I GGNCC 1 cut(s) 481
SchI GAGTC 2 cut(s) 593, 776
SfaNI GCATC 3 cut(s) 368, 511, 703
SmiMI CAYNNNNRTG 4 cut(s) 444, 519, 699, 820
SmlI CTYRAG 1 cut(s) 307
SmoI CTYRAG 1 cut(s) 307
Sse9I AATT 1 cut(s) 742
SsiI CCGC 5 cut(s) 162, 432, 504, 527, 593
SspMI CTAG 4 cut(s) 81, 240, 470, 617
StyI CCWWGG 2 cut(s) 239, 564
TaaI ACNGT 2 cut(s) 88, 751
TaiI ACGT 5 cut(s) 63, 200, 222, 343, 789
TaqI TCGA 2 cut(s) 123, 687
TasI AATT 1 cut(s) 742
TauI GCSGC 1 cut(s) 164
TfiI GAWTC 1 cut(s) 367
Tru1I TTAA 2 cut(s) 300, 336
Tru9I TTAA 2 cut(s) 300, 336
TscAI CASTG 2 cut(s) 93, 546
TseI GCWGC 3 cut(s) 52, 158, 524
TspDTI ATGAA 2 cut(s) 269, 370
TspGWI ACGGA 1 cut(s) 352
TspRI CASTG 2 cut(s) 93, 546
XceI RCATGY 2 cut(s) 332, 868
XcmI CCANNNNNNNNNTGG 1 cut(s) 571
XmaJI CCTAGG 1 cut(s) 239
XmnI GAANNNNTTC 1 cut(s) 714
XspI CTAG 4 cut(s) 81, 240, 470, 617
Zsp2I ATGCAT 1 cut(s) 866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.