Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0004889

Physical Location & Seq

Genomic Coordinates Reverse (-)

18644061 .. 18646606

Gene Structure 2546 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G45220.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1536 bp

ATGATGGCTTTTCGAGCTTATATTATTAACTTTGTGATCTTATGCATTCTCGTGGCATCGACCGTTTCCGGGTACAACCAGAAAGACGTGAAAGCATGGTGCAGCCAAACTCCAAACCCTAAACCTTGCGAGTATTTCTTAACCCATAACTCAAACAATGAACCCATAAAGTCTGAATCCGAGTTTCTCAAAATCTCAATGAAACTCGTATTAGACCGAGCCATCCTCGCCAAAACCCACGCGTTCACACTAGGACCAAAGTGCCGTGACACACGTGAAAAAGCAGCGTGGGAAGATTGTATCAAGCTCTACGACCTCACCGTCTCTAAAATCAACGAGACAATGGACCCAAACGTGAAGTGCTCAAAACTCGACGCGCAAACATGGCTAAGCACGGCTCTAACCAATCTTGACACATGTCGAGCTGGGTTCTTAGAGCTTGGTGTTACCGATATTGTCCTTCCTTTGATGTCAAACAACGTCTCGAATCTTCTATGCAACACACTCGCCATTAACAAAGTTCCTTTCAACTACACCCCACCCGAGAAAGACGGGTTCCCTTCGTGGGTCAAACCTGGTGACCGGAAACTTTTGCAAAGCTCGACGCCAAAAGATAACGCAGTGGTGGCGAAAGACGGATCTGGCAATTTTAAGACGATAAAAGAAGCGATTGATGCTGCTTCGGGGAGTGGTAGGTTCGTTATATATGTGAAGCAAGGCGTTTACAGTGAGAACCTTGAGATTCGGAAAAAGAATGTCATGTTGAGAGGTGATGGTATAGGGAAGACGATTATTACCGGAAGCAAAAGTGTTGGAGGAGGAACGACAACCTTTAACTCAGCTACTGTCGCGGCGGTCGGAGACGGGTTTATAGCTCGTGGGATCACGTTTCGAAACACGGCGGGTGCAAGCAACGAACAAGCGGTAGCTCTAAGATCCGGGTCGGATCTGTCAGTTTTTTACCAATGCAGTTTCGAAGCTTACCAAGACACACTCTACGTTCACTCCAACCGTCAGTTTTACCGCGATTGCGACGTTTATGGAACTGTCGATTTTATCTTCGGAAACGCAGCCGCGGTTCTCCAGAACTGCAACATTTTCGCACGTCGTCCTCGTAGTAAGACCAACACAATCACAGCTCAAGGAAGATCCGACCCGAATCAAAACACGGGGATTATTATCCACAACTCTCGGGTCACGGCCGCTTCGGATCTTCGACCCGTTTTGGGATCCACAAAGACTTATTTGGGTAGACCTTGGAGACAATATTCTAGGACGGTTTTTATGAAGACTTCTCTTGATAGTTTGATTGATCCACGTGGCTGGCTCGAGTGGGATGGTAATTTCGCTTTGAAGACTTTGTTCTATGCTGAGTTTCAAAATACGGGTCCGGGTGCTTCAACTTCGGGTCGGGTCACATGGCCAGGGTTTCGGGTCCTTGGTTCTGCTTCAGAGGCTTCCAAGTTTACTGTTGGTACTTTCCTCGCCGGTGGCTCTTGGATTCCATCTAGCGTGCCGTTCACTTCTGGTCTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0016020 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0042742 GO:0043086 GO:0043207 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0050829 GO:0050896 GO:0051704 GO:0051707 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098542 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

511

Amino Acids

55.98

Weight (kDa)

9.2

Isoelectric Point (pI)

25.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	29 - 171	1.4e-27	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	206 - 497	6.3e-140	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004889)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G45220
fragaria_vesca	FvH4_7g11320 FvH4_7g27760
malus_domestica	MD02G1207900.v1.1 MD07G1255000.v1.1 MD11G1195500.v1.1
prunus_persica	Prupe.2G141000_v2.0.a1 Prupe.2G278200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0349521 RchiOBHm_Chr1g0374551
rosa_laevigata	RLG00000026729 RLG00000028541 RLG00000028544
rosa_multiflora	Rmu_sc0002495.1_g000016 Rmu_sc0002495.1_g000029 Rmu_sc0004947.1_g000001
rosa_roxburghii	Rroxscaffold_4G00283410 Rroxscaffold_4G00305490
rosa_rugosa	Rorug01G0204300 Rorug01G0384100
rosa_samantha	Rh1AG394800 Rh1BG187700 Rh1BG359500 Rh1CG206500 Rh1CG372600 Rh1DG217200 Rh1DG390100
rosa_wichuraiana	Rw1G018840 Rw1G018870 Rw1G035010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	320
AccI	GTMKAC	1 cut(s)	1252
AccII	CGCG	5 cut(s)	242, 377, 851, 1026, 1076
AciI	CCGC	8 cut(s)	851, 854, 902, 923, 1024, 1074, 1076, 1203
AclWI	GGATC	9 cut(s)	646, 890, 930, 954, 1143, 1218, 1224, 1237, 1307
AcoI	YGGCCR	2 cut(s)	1200, 1421
AcuI	CTGAAG	1 cut(s)	1434
AcvI	CACGTG	2 cut(s)	275, 1319
AcyI	GRCGYC	1 cut(s)	605
AfaI	GTAC	2 cut(s)	74, 1477
AfiI	CCNNNNNNNGG	3 cut(s)	69, 565, 1226
AflIII	ACRYGT	3 cut(s)	240, 272, 416
AgsI	TTSAA	4 cut(s)	529, 1354, 1379, 1401
AjiI	CACGTC	2 cut(s)	88, 1106
AjnI	CCWGG	2 cut(s)	574, 1423
Alw21I	GWGCWC	1 cut(s)	365
Alw26I	GTCTC	5 cut(s)	328, 332, 487, 855, 1255
AlwI	GGATC	9 cut(s)	646, 890, 930, 954, 1143, 1218, 1224, 1237, 1307
AlwNI	CAGNNNCTG	1 cut(s)	845
Ama87I	CYCGRG	3 cut(s)	542, 1191, 1328
AoxI	GGCC	2 cut(s)	1200, 1421
ApeKI	GCWGC	4 cut(s)	102, 284, 677, 1070
ArsI	GACNNNNNNTTYG	2 cut(s)	1372, 1404
AspLEI	GCGC	1 cut(s)	379
AspS9I	GGNCC	4 cut(s)	254, 346, 1388, 1435
AsuC2I	CCSGG	3 cut(s)	70, 940, 1392
AsuHPI	GGTGA	3 cut(s)	310, 590, 782
AsuII	TTCGAA	2 cut(s)	892, 975
AvaI	CYCGRG	3 cut(s)	542, 1191, 1328
AvaII	GGWCC	4 cut(s)	254, 346, 1388, 1435
BalI	TGGCCA	1 cut(s)	1423
BamHI	GGATCC	1 cut(s)	1229
BauI	CACGAG	2 cut(s)	50, 876
BbrPI	CACGTG	2 cut(s)	275, 1319
BbsI	GAAGAC	3 cut(s)	791, 1295, 1361
Bbv12I	GWGCWC	1 cut(s)	365
BbvI	GCAGC	4 cut(s)	114, 296, 664, 1082
BccI	CCATC	4 cut(s)	230, 767, 1331, 1513
BceAI	ACGGC	5 cut(s)	249, 411, 915, 1215, 1501
BcgI	CGANNNNNNTGC	4 cut(s)	487, 521, 1081, 1115
BciT130I	CCWGG	2 cut(s)	576, 1425
BcnI	CCSGG	3 cut(s)	70, 940, 1392
BcoDI	GTCTC	5 cut(s)	328, 332, 487, 855, 1255
BfaI	CTAG	4 cut(s)	251, 1272, 1509, 1534
BisI	GCNGC	7 cut(s)	103, 285, 678, 852, 1071, 1074, 1203
BlpI	GCTNAGC	1 cut(s)	389
BlsI	GCNGC	7 cut(s)	104, 286, 679, 853, 1072, 1075, 1204
Bme1390I	CCNGG	5 cut(s)	70, 576, 940, 1392, 1425
Bme18I	GGWCC	4 cut(s)	254, 346, 1388, 1435
BmeT110I	CYCGRG	3 cut(s)	542, 1191, 1328
BmgBI	CACGTC	2 cut(s)	88, 1106
BmgT120I	GGNCC	4 cut(s)	254, 346, 1388, 1435
BmiI	GGNNCC	5 cut(s)	348, 557, 1231, 1389, 1436
BmrFI	CCNGG	5 cut(s)	70, 576, 940, 1392, 1425
BmsI	GCATC	2 cut(s)	65, 664
BoxI	GACNNNNGTC	1 cut(s)	417
BpiI	GAAGAC	3 cut(s)	791, 1295, 1361
BplI	GAGNNNNNCTC	2 cut(s)	210, 242
BpmI	CTGGAG	1 cut(s)	1067
Bpu1102I	GCTNAGC	1 cut(s)	389
Bpu14I	TTCGAA	2 cut(s)	892, 975
BpuEI	CTTGAG	2 cut(s)	758, 1125
BpuMI	CCSGG	3 cut(s)	70, 940, 1392
BsaAI	YACGTR	2 cut(s)	275, 1319
BsaBI	GATNNNNATC	1 cut(s)	1178
BsaHI	GRCGYC	1 cut(s)	605
BsaJI	CCNNGG	4 cut(s)	1074, 1256, 1424, 1438
BsaWI	WCCGGW	2 cut(s)	582, 797
BsaXI	ACNNNNNCTCC	2 cut(s)	989, 1019
Bsc4I	CCNNNNNNNGG	3 cut(s)	69, 565, 1226
Bse118I	RCCGGY	1 cut(s)	1487
Bse8I	GATNNNNATC	1 cut(s)	1178
BseBI	CCWGG	2 cut(s)	576, 1425
BseDI	CCNNGG	4 cut(s)	1074, 1256, 1424, 1438
BseGI	GGATG	2 cut(s)	222, 1342
BseJI	GATNNNNATC	1 cut(s)	1178
BseLI	CCNNNNNNNGG	3 cut(s)	69, 565, 1226
BseMII	CTCAG	2 cut(s)	852, 1362
BseRI	GAGGAG	1 cut(s)	831
BseX3I	CGGCCG	1 cut(s)	1200
BseXI	GCAGC	4 cut(s)	114, 296, 664, 1082
BseYI	CCCAGC	1 cut(s)	425
BsgI	GTGCAG	1 cut(s)	121
Bsh1236I	CGCG	5 cut(s)	242, 377, 851, 1026, 1076
Bsh1285I	CGRYCG	3 cut(s)	63, 858, 1203
BshFI	GGCC	2 cut(s)	1202, 1423
BsiEI	CGRYCG	3 cut(s)	63, 858, 1203
BsiHKAI	GWGCWC	1 cut(s)	365
BsiHKCI	CYCGRG	3 cut(s)	542, 1191, 1328
BsiSI	CCGG	6 cut(s)	69, 583, 798, 939, 1391, 1488
BslI	CCNNNNNNNGG	3 cut(s)	69, 565, 1226
BsmAI	GTCTC	5 cut(s)	328, 332, 487, 855, 1255
BsmBI	CGTCTC	3 cut(s)	328, 487, 855
BsmI	GAATGC	1 cut(s)	45
BsnI	GGCC	2 cut(s)	1202, 1423
BsoBI	CYCGRG	3 cut(s)	542, 1191, 1328
Bsp119I	TTCGAA	2 cut(s)	892, 975
Bsp1286I	GDGCHC	1 cut(s)	365
Bsp143I	GATC	9 cut(s)	36, 638, 882, 935, 946, 1148, 1210, 1229, 1312
Bsp1720I	GCTNAGC	1 cut(s)	389
BspACI	CCGC	8 cut(s)	851, 854, 902, 923, 1024, 1074, 1076, 1203
BspANI	GGCC	2 cut(s)	1202, 1423
BspCNI	CTCAG	2 cut(s)	851, 1363
BspFNI	CGCG	5 cut(s)	242, 377, 851, 1026, 1076
BspLI	GGNNCC	5 cut(s)	348, 557, 1231, 1389, 1436
BspPI	GGATC	9 cut(s)	646, 890, 930, 954, 1143, 1218, 1224, 1237, 1307
BspT104I	TTCGAA	2 cut(s)	892, 975
BsrFI	RCCGGY	1 cut(s)	1487
BssAI	RCCGGY	1 cut(s)	1487
BssECI	CCNNGG	4 cut(s)	1074, 1256, 1424, 1438
BssMI	GATC	9 cut(s)	36, 638, 882, 935, 946, 1148, 1210, 1229, 1312
BssNI	GRCGYC	1 cut(s)	605
BssSI	CACGAG	2 cut(s)	50, 876
BssT1I	CCWWGG	2 cut(s)	1256, 1438
Bst2BI	CACGAG	2 cut(s)	50, 876
Bst2UI	CCWGG	2 cut(s)	576, 1425
Bst4CI	ACNGT	8 cut(s)	64, 322, 728, 847, 1013, 1048, 1279, 1471
BstACI	GRCGYC	1 cut(s)	605
BstBAI	YACGTR	2 cut(s)	275, 1319
BstBI	TTCGAA	2 cut(s)	892, 975
BstC8I	GCNNGC	3 cut(s)	910, 1325, 1514
BstDEI	CTNAG	5 cut(s)	389, 433, 838, 932, 1371
BstDSI	CCRYGG	1 cut(s)	1074
BstEII	GGTNACC	1 cut(s)	578
BstF5I	GGATG	2 cut(s)	222, 1342
BstFNI	CGCG	5 cut(s)	242, 377, 851, 1026, 1076
BstHHI	GCGC	1 cut(s)	379
BstKTI	GATC	9 cut(s)	39, 641, 885, 938, 949, 1151, 1213, 1232, 1315
BstMAI	GTCTC	5 cut(s)	328, 332, 487, 855, 1255
BstMBI	GATC	9 cut(s)	36, 638, 882, 935, 946, 1148, 1210, 1229, 1312
BstMCI	CGRYCG	3 cut(s)	63, 858, 1203
BstMWI	GCNNNNNNNGC	7 cut(s)	14, 227, 385, 626, 674, 848, 1454
BstNI	CCWGG	2 cut(s)	576, 1425
BstNSI	RCATGY	1 cut(s)	420
BstPAI	GACNNNNGTC	1 cut(s)	417
BstPI	GGTNACC	1 cut(s)	578
BstSCI	CCNGG	5 cut(s)	68, 574, 938, 1390, 1423
BstUI	CGCG	5 cut(s)	242, 377, 851, 1026, 1076
BstV1I	GCAGC	4 cut(s)	114, 296, 664, 1082
BstV2I	GAAGAC	3 cut(s)	791, 1295, 1361
BstX2I	RGATCY	6 cut(s)	638, 935, 946, 1148, 1210, 1229
BstXI	CCANNNNNNTGG	1 cut(s)	1323
BstYI	RGATCY	6 cut(s)	638, 935, 946, 1148, 1210, 1229
BstZI	CGGCCG	1 cut(s)	1200
BsuRI	GGCC	2 cut(s)	1202, 1423
BtgI	CCRYGG	1 cut(s)	1074
BtrI	CACGTC	2 cut(s)	88, 1106
BtsCI	GGATG	2 cut(s)	222, 1342
BtsI	GCAGTG	1 cut(s)	627
BtsIMutI	CAGTG	2 cut(s)	627, 733
Cac8I	GCNNGC	3 cut(s)	910, 1325, 1514
CaiI	CAGNNNCTG	1 cut(s)	845
CfoI	GCGC	1 cut(s)	379
Cfr10I	RCCGGY	1 cut(s)	1487
Cfr13I	GGNCC	4 cut(s)	254, 346, 1388, 1435
Cfr42I	CCGCGG	1 cut(s)	1077
CseI	GACGC	2 cut(s)	383, 613
CsiI	ACCWGGT	1 cut(s)	574
Csp6I	GTAC	2 cut(s)	73, 1476
CviAII	CATG	5 cut(s)	96, 384, 417, 760, 1419
CviQI	GTAC	2 cut(s)	73, 1476
DdeI	CTNAG	5 cut(s)	389, 433, 838, 932, 1371
DpnI	GATC	9 cut(s)	38, 640, 884, 937, 948, 1150, 1212, 1231, 1314
DpnII	GATC	9 cut(s)	36, 638, 882, 935, 946, 1148, 1210, 1229, 1312
DrdI	GACNNNNNNGTC	1 cut(s)	320
DseDI	GACNNNNNNGTC	1 cut(s)	320
EaeI	YGGCCR	2 cut(s)	1200, 1421
EagI	CGGCCG	1 cut(s)	1200
EclXI	CGGCCG	1 cut(s)	1200
Eco130I	CCWWGG	2 cut(s)	1256, 1438
Eco47I	GGWCC	4 cut(s)	254, 346, 1388, 1435
Eco52I	CGGCCG	1 cut(s)	1200
Eco57I	CTGAAG	1 cut(s)	1434
Eco72I	CACGTG	2 cut(s)	275, 1319
Eco88I	CYCGRG	3 cut(s)	542, 1191, 1328
Eco91I	GGTNACC	1 cut(s)	578
EcoO109I	RGGNCCY	1 cut(s)	1435
EcoO65I	GGTNACC	1 cut(s)	578
EcoRII	CCWGG	2 cut(s)	574, 1423
EcoT14I	CCWWGG	2 cut(s)	1256, 1438
EcoT22I	ATGCAT	1 cut(s)	47
ErhI	CCWWGG	2 cut(s)	1256, 1438
Esp3I	CGTCTC	3 cut(s)	328, 487, 855
FaeI	CATG	5 cut(s)	99, 387, 420, 763, 1422
FatI	CATG	5 cut(s)	95, 383, 416, 759, 1418
FauI	CCCGC	1 cut(s)	895
FblI	GTMKAC	1 cut(s)	1252
Fnu4HI	GCNGC	7 cut(s)	103, 285, 678, 852, 1071, 1074, 1203
FokI	GGATG	2 cut(s)	209, 1349
Fsp4HI	GCNGC	7 cut(s)	103, 285, 678, 852, 1071, 1074, 1203
FspBI	CTAG	4 cut(s)	251, 1272, 1509, 1534
GlaI	GCGC	1 cut(s)	378
GluI	GCNGC	7 cut(s)	103, 285, 678, 852, 1071, 1074, 1203
GsaI	CCCAGC	1 cut(s)	429
GsuI	CTGGAG	1 cut(s)	1067
HaeIII	GGCC	2 cut(s)	1202, 1423
HapII	CCGG	6 cut(s)	69, 583, 798, 939, 1391, 1488
HgaI	GACGC	2 cut(s)	383, 613
HhaI	GCGC	1 cut(s)	379
Hin1I	GRCGYC	1 cut(s)	605
Hin1II	CATG	5 cut(s)	99, 387, 420, 763, 1422
Hin6I	GCGC	1 cut(s)	377
HinP1I	GCGC	1 cut(s)	377
HindIII	AAGCTT	1 cut(s)	978
HinfI	GANTC	5 cut(s)	176, 487, 742, 1159, 1501
HpaII	CCGG	6 cut(s)	69, 583, 798, 939, 1391, 1488
HphI	GGTGA	3 cut(s)	310, 590, 782
Hpy166II	GTNNAC	6 cut(s)	246, 724, 1003, 1253, 1467, 1521
Hpy188I	TCNGA	9 cut(s)	175, 181, 747, 860, 946, 1064, 1153, 1210, 1453
Hpy188III	TCNNGA	4 cut(s)	410, 484, 1084, 1298
Hpy8I	GTNNAC	6 cut(s)	246, 724, 1003, 1253, 1467, 1521
Hpy99I	CGWCG	4 cut(s)	377, 607, 1037, 1110
HpyAV	CCTTC	2 cut(s)	470, 570
HpyCH4III	ACNGT	8 cut(s)	64, 322, 728, 847, 1013, 1048, 1279, 1471
HpyCH4IV	ACGT	9 cut(s)	87, 274, 354, 480, 887, 999, 1035, 1105, 1318
HpyCH4V	TGCA	7 cut(s)	45, 102, 498, 595, 908, 969, 1092
HpyF10VI	GCNNNNNNNGC	7 cut(s)	14, 227, 385, 626, 674, 848, 1454
HpyF3I	CTNAG	5 cut(s)	389, 433, 838, 932, 1371
HpySE526I	ACGT	9 cut(s)	87, 274, 354, 480, 887, 999, 1035, 1105, 1318
Hsp92I	GRCGYC	1 cut(s)	605
Hsp92II	CATG	5 cut(s)	99, 387, 420, 763, 1422
HspAI	GCGC	1 cut(s)	377
KspI	CCGCGG	1 cut(s)	1077
Kzo9I	GATC	9 cut(s)	36, 638, 882, 935, 946, 1148, 1210, 1229, 1312
Lsp1109I	GCAGC	4 cut(s)	114, 296, 664, 1082
LweI	GCATC	2 cut(s)	65, 664
MabI	ACCWGGT	1 cut(s)	574
MaeI	CTAG	4 cut(s)	251, 1272, 1509, 1534
MaeII	ACGT	9 cut(s)	87, 274, 354, 480, 887, 999, 1035, 1105, 1318
MaeIII	GTNAC	5 cut(s)	266, 445, 578, 1195, 1414
MalI	GATC	9 cut(s)	38, 640, 884, 937, 948, 1150, 1212, 1231, 1314
MboI	GATC	9 cut(s)	36, 638, 882, 935, 946, 1148, 1210, 1229, 1312
MboII	GAAGA	8 cut(s)	305, 482, 796, 1051, 1158, 1205, 1300, 1366
MflI	RGATCY	6 cut(s)	638, 935, 946, 1148, 1210, 1229
MhlI	GDGCHC	1 cut(s)	365
MlsI	TGGCCA	1 cut(s)	1423
MluCI	AATT	2 cut(s)	646, 1342
MluI	ACGCGT	1 cut(s)	240
MluNI	TGGCCA	1 cut(s)	1423
MmeI	TCCRAC	5 cut(s)	793, 838, 924, 1032, 1176
MnlI	CCTC	8 cut(s)	236, 326, 761, 809, 812, 1122, 1447, 1493
Mox20I	TGGCCA	1 cut(s)	1423
Mph1103I	ATGCAT	1 cut(s)	47
MscI	TGGCCA	1 cut(s)	1423
MseI	TTAA	5 cut(s)	27, 140, 513, 651, 834
MslI	CAYNNNNRTG	1 cut(s)	50
Msp20I	TGGCCA	1 cut(s)	1423
MspA1I	CMGCKG	1 cut(s)	1076
MspI	CCGG	6 cut(s)	69, 583, 798, 939, 1391, 1488
MspR9I	CCNGG	5 cut(s)	70, 576, 940, 1392, 1425
Mva1269I	GAATGC	1 cut(s)	45
MvaI	CCWGG	2 cut(s)	576, 1425
MvnI	CGCG	5 cut(s)	242, 377, 851, 1026, 1076
MwoI	GCNNNNNNNGC	7 cut(s)	14, 227, 385, 626, 674, 848, 1454
NciI	CCSGG	3 cut(s)	70, 940, 1392
NdeII	GATC	9 cut(s)	36, 638, 882, 935, 946, 1148, 1210, 1229, 1312
NlaIII	CATG	5 cut(s)	99, 387, 420, 763, 1422
NlaIV	GGNNCC	5 cut(s)	348, 557, 1231, 1389, 1436
NmuCI	GTSAC	4 cut(s)	266, 578, 1195, 1414
NsiI	ATGCAT	1 cut(s)	47
NspI	RCATGY	1 cut(s)	420
NspV	TTCGAA	2 cut(s)	892, 975
PaeR7I	CTCGAG	1 cut(s)	1328
PciI	ACATGT	1 cut(s)	416
PcsI	WCGNNNNNNNCGW	2 cut(s)	318, 1111
PctI	GAATGC	1 cut(s)	45
PfeI	GAWTC	5 cut(s)	176, 487, 742, 1159, 1501
PkrI	GCNGC	7 cut(s)	104, 286, 679, 853, 1072, 1075, 1204
PmaCI	CACGTG	2 cut(s)	275, 1319
PmlI	CACGTG	2 cut(s)	275, 1319
Ppu21I	YACGTR	2 cut(s)	275, 1319
PpuMI	RGGWCCY	1 cut(s)	1435
PscI	ACATGT	1 cut(s)	416
PshAI	GACNNNNGTC	1 cut(s)	417
Psp5II	RGGWCCY	1 cut(s)	1435
Psp6I	CCWGG	2 cut(s)	574, 1423
PspCI	CACGTG	2 cut(s)	275, 1319
PspEI	GGTNACC	1 cut(s)	578
PspFI	CCCAGC	1 cut(s)	425
PspGI	CCWGG	2 cut(s)	574, 1423
PspN4I	GGNNCC	5 cut(s)	348, 557, 1231, 1389, 1436
PspPI	GGNCC	4 cut(s)	254, 346, 1388, 1435
PspPPI	RGGWCCY	1 cut(s)	1435
PspXI	VCTCGAGB	1 cut(s)	1328
PstNI	CAGNNNCTG	1 cut(s)	845
PsuI	RGATCY	6 cut(s)	638, 935, 946, 1148, 1210, 1229
RsaI	GTAC	2 cut(s)	74, 1477
RsaNI	GTAC	2 cut(s)	73, 1476
RseI	CAYNNNNRTG	1 cut(s)	50
SacII	CCGCGG	1 cut(s)	1077
SaqAI	TTAA	5 cut(s)	27, 140, 513, 651, 834
SatI	GCNGC	7 cut(s)	103, 285, 678, 852, 1071, 1074, 1203
Sau3AI	GATC	9 cut(s)	36, 638, 882, 935, 946, 1148, 1210, 1229, 1312
Sau96I	GGNCC	4 cut(s)	254, 346, 1388, 1435
ScrFI	CCNGG	5 cut(s)	70, 576, 940, 1392, 1425
SduI	GDGCHC	1 cut(s)	365
SexAI	ACCWGGT	1 cut(s)	574
SfaNI	GCATC	2 cut(s)	65, 664
Sfr274I	CTCGAG	1 cut(s)	1328
Sfr303I	CCGCGG	1 cut(s)	1077
SfuI	TTCGAA	2 cut(s)	892, 975
SgrAI	CRCCGGYG	1 cut(s)	1487
SgrBI	CCGCGG	1 cut(s)	1077
SinI	GGWCC	4 cut(s)	254, 346, 1388, 1435
SlaI	CTCGAG	1 cut(s)	1328
SmiMI	CAYNNNNRTG	1 cut(s)	50
SmlI	CTYRAG	3 cut(s)	737, 1140, 1328
SmoI	CTYRAG	3 cut(s)	737, 1140, 1328
Sse9I	AATT	2 cut(s)	646, 1342
SsiI	CCGC	8 cut(s)	851, 854, 902, 923, 1024, 1074, 1076, 1203
SspI	AATATT	1 cut(s)	1268
SspMI	CTAG	4 cut(s)	251, 1272, 1509, 1534
StyD4I	CCNGG	5 cut(s)	68, 574, 938, 1390, 1423
StyI	CCWWGG	2 cut(s)	1256, 1438
TaaI	ACNGT	8 cut(s)	64, 322, 728, 847, 1013, 1048, 1279, 1471
TaiI	ACGT	9 cut(s)	90, 277, 357, 483, 890, 1002, 1038, 1108, 1321
TaqII	GACCGA	1 cut(s)	231
TasI	AATT	2 cut(s)	646, 1342
TauI	GCSGC	3 cut(s)	854, 1076, 1205
TfiI	GAWTC	5 cut(s)	176, 487, 742, 1159, 1501
Tru1I	TTAA	5 cut(s)	27, 140, 513, 651, 834
Tru9I	TTAA	5 cut(s)	27, 140, 513, 651, 834
TscAI	CASTG	2 cut(s)	627, 733
TseFI	GTSAC	4 cut(s)	266, 578, 1195, 1414
TseI	GCWGC	4 cut(s)	102, 284, 677, 1070
Tsp45I	GTSAC	4 cut(s)	266, 578, 1195, 1414
TspDTI	ATGAA	3 cut(s)	174, 215, 1301
TspGWI	ACGGA	1 cut(s)	651
TspRI	CASTG	2 cut(s)	627, 733
VpaK11BI	GGWCC	4 cut(s)	254, 346, 1388, 1435
XceI	RCATGY	1 cut(s)	420
XhoI	CTCGAG	1 cut(s)	1328
XmiI	GTMKAC	1 cut(s)	1252
XspI	CTAG	4 cut(s)	251, 1272, 1509, 1534
Zsp2I	ATGCAT	1 cut(s)	47

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.