Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb7

Orthogroup ID

OG0004889

Physical Location & Seq

Genomic Coordinates Reverse (-)

10533675 .. 10536206

Gene Structure 2532 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_7g11320.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1527 bp

ATGGCTGCTCGCATTTTCTTAACACTTTGTTTTGTGCTTTATCTTTCTCCATTCGTTCTTGGTTACTCTGCCAATGATATCAAAACATGGTGCAGCAAAACTCCAAACCCAAAACCATGCGAGGAACTTCTGAGCCATAACAAAAATCAAATCCCCATCAAACAAAAATCCCAGTTCTTCCAGATATCTACCCAGCTAGCCCTAGACCGAGCCATGCATGCTCACACCAACTTTCTTTCGCTAGGCTCAAAGTTCGTAAATCCACGAGCAAAAGCTGCATGGGACGATTGTCTTGAGCTCTATGACCTCACCATCCAGAAGCTCAACACAACCGTAAACCCTAGCACCAAGTGCACCCAGGAGGACACTCAGACATGGCTCAGCACGGCTCTCACAAACCTCGAGACATGCCGAGAAGGGTTTTCGGAGCTCGGGGTGTCGGATTTCTTTCTACCTGTGATGTCGAATAACGTTTCGAATTTGATCAGCAACACTCTTTCCATTAACTACGTACCCTACAACCCGGCGCCGCCTTCCAAAGACGGGTTTCCGACTTGGGTTAACCCCGGTAACCGAAGGCTCCTGCAGTCAAGTTCTCCGACGGCGAATCTCGTAGTGGCCAAAGACGGGTCCGGGAATTACAAGACGGTGACCGAGGCAATAACTACCGCATCGAAGCGGTCGGGCACCGCAAGGTATGTGATATATGTGAAGGCAGGGACATACAAGGAGAACGTCGTGATAACGTCAAAGAATATTATGTTGATGGGAGATGGCATCGGGAAGACTATTATCACAGGGAGCAAAAGTGTTGGAGGAGGTTCTACAACCTTCAATTCAGCTACAGTGGCTGTAACCGGAGATGGGTTCATTGCTCGTGACATTACATTCCAAAACACTGCCGGTGTATCAAACAACCAAGCCGTGGCGCTCCGTTCCGGTTCAGATCTTTCAGTGTTCTACAAGTGCAGCTTTGAGGGGTACCAAGACACCCTGTATGTCTACTCCGACAGGCAATTCTACAGAGAGTGCAATATTTATGGAACCGTCGACTTCATTTTCGGCAACGCTGCTGTGGTTTTACAAAACTGTAACATTTATGTACACAACCACAAGACCAACACCGTTACGGCTCAAGGTCGGAGCGACCCTAACCAGAACACAGGGATTTCCATTCATAACTGTAAGGTTACGGCTGCTTCAGATTTAGTCCTAAGTTCAACCAAAACCTACCTAGGAAGACCATGGAGGGAGTATTCTCGAACAATTTTTATGAAGACTTACCTTGACAGCTTGATTAACTCGGCTGGTTGGTTGGAATGGGATGGTACTTTTGCCCTAGAGACTCTGTACTATGGTGAGTATATGAACACTGGCCCTGGTTCATCCACTTCTGGTAGGGTTAAGTGGGGAGGTTACCATGTCATAACTAGTTCTTCGGAGGCTTCCAAGTTCACTGTTGGTAACTTCATTGCTGGTAGCTCTTGGTTGCCAGCTACCAATGTGCCTTTCACCGCTGGTTTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

509

Amino Acids

55.69

Weight (kDa)

8.73

Isoelectric Point (pI)

32.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	26 - 168	1.7e-30	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	203 - 493	1.4e-136	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004889)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G45220
fragaria_vesca	FvH4_7g11320 FvH4_7g27760
malus_domestica	MD02G1207900.v1.1 MD07G1255000.v1.1 MD11G1195500.v1.1
prunus_persica	Prupe.2G141000_v2.0.a1 Prupe.2G278200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0349521 RchiOBHm_Chr1g0374551
rosa_laevigata	RLG00000026729 RLG00000028541 RLG00000028544
rosa_multiflora	Rmu_sc0002495.1_g000016 Rmu_sc0002495.1_g000029 Rmu_sc0004947.1_g000001
rosa_roxburghii	Rroxscaffold_4G00283410 Rroxscaffold_4G00305490
rosa_rugosa	Rorug01G0204300 Rorug01G0384100
rosa_samantha	Rh1AG394800 Rh1BG187700 Rh1BG359500 Rh1CG206500 Rh1CG372600 Rh1DG217200 Rh1DG390100
rosa_wichuraiana	Rw1G018840 Rw1G018870 Rw1G035010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1525
Acc65I	GGTACC	1 cut(s)	981
AccB1I	GGYRCC	3 cut(s)	526, 686, 981
AccB7I	CCANNNNNTGG	1 cut(s)	925
AccI	GTMKAC	2 cut(s)	1002, 1050
AciI	CCGC	5 cut(s)	530, 669, 679, 690, 1515
AclI	AACGTT	1 cut(s)	471
AcoI	YGGCCR	1 cut(s)	618
AcsI	RAATTY	1 cut(s)	478
AcuI	CTGAAG	1 cut(s)	1185
AcyI	GRCGYC	1 cut(s)	527
AdeI	CACNNNGTG	1 cut(s)	351
AfaI	GTAC	5 cut(s)	513, 983, 1104, 1330, 1352
AfiI	CCNNNNNNNGG	4 cut(s)	543, 627, 864, 925
AgsI	TTSAA	2 cut(s)	835, 1221
AhlI	ACTAGT	1 cut(s)	1430
AjnI	CCWGG	2 cut(s)	357, 1378
AjuI	GAANNNNNNNTTGG	2 cut(s)	1493, 1525
Alw21I	GWGCWC	3 cut(s)	300, 356, 432
Alw26I	GTCTC	2 cut(s)	398, 1337
Alw44I	GTGCAC	1 cut(s)	352
AlwNI	CAGNNNCTG	1 cut(s)	851
Ama87I	CYCGRG	2 cut(s)	401, 431
AoxI	GGCC	2 cut(s)	618, 1375
ApaLI	GTGCAC	1 cut(s)	352
ApeKI	GCWGC	6 cut(s)	5, 93, 275, 969, 1070, 1196
ApoI	RAATTY	1 cut(s)	478
ArsI	GACNNNNNNTTYG	2 cut(s)	1043, 1075
Asp718I	GGTACC	1 cut(s)	981
AspA2I	CCTAGG	1 cut(s)	1234
AspLEI	GCGC	2 cut(s)	529, 931
AspS9I	GGNCC	2 cut(s)	630, 1376
AsuC2I	CCSGG	3 cut(s)	524, 567, 634
AsuHPI	GGTGA	4 cut(s)	301, 661, 1370, 1504
AsuII	TTCGAA	1 cut(s)	476
AsuNHI	GCTAGC	1 cut(s)	196
AvaI	CYCGRG	2 cut(s)	401, 431
AvaII	GGWCC	1 cut(s)	630
AvrII	CCTAGG	1 cut(s)	1234
BaeGI	GKGCMC	2 cut(s)	356, 689
BalI	TGGCCA	1 cut(s)	620
BanI	GGYRCC	3 cut(s)	526, 686, 981
BanII	GRGCYC	2 cut(s)	300, 432
BauI	CACGAG	2 cut(s)	264, 876
BbsI	GAAGAC	3 cut(s)	791, 1246, 1283
Bbv12I	GWGCWC	3 cut(s)	300, 356, 432
BbvI	GCAGC	5 cut(s)	105, 262, 981, 1057, 1183
BccI	CCATC	6 cut(s)	164, 320, 760, 767, 857, 1319
BceAI	ACGGC	5 cut(s)	402, 618, 908, 1146, 1209
BciT130I	CCWGG	2 cut(s)	359, 1380
BclI	TGATCA	1 cut(s)	483
BcnI	CCSGG	3 cut(s)	524, 567, 634
BcoDI	GTCTC	2 cut(s)	398, 1337
BcuI	ACTAGT	1 cut(s)	1430
BfaI	CTAG	7 cut(s)	197, 203, 242, 342, 1235, 1340, 1431
BfmI	CTRYAG	3 cut(s)	584, 843, 1021
BfoI	RGCGCY	2 cut(s)	530, 932
BglII	AGATCT	1 cut(s)	946
BisI	GCNGC	7 cut(s)	6, 94, 276, 530, 970, 1071, 1197
BlnI	CCTAGG	1 cut(s)	1234
BlpI	GCTNAGC	1 cut(s)	380
BlsI	GCNGC	7 cut(s)	7, 95, 277, 531, 971, 1072, 1198
Bme1390I	CCNGG	5 cut(s)	359, 524, 567, 634, 1380
Bme18I	GGWCC	1 cut(s)	630
BmeT110I	CYCGRG	2 cut(s)	401, 431
BmgT120I	GGNCC	2 cut(s)	630, 1376
BmiI	GGNNCC	6 cut(s)	528, 581, 631, 688, 983, 1045
BmrFI	CCNGG	5 cut(s)	359, 524, 567, 634, 1380
BmrI	ACTGGG	1 cut(s)	166
BmsI	GCATC	2 cut(s)	680, 786
BmtI	GCTAGC	1 cut(s)	200
BmuI	ACTGGG	1 cut(s)	166
BoxI	GACNNNNGTC	1 cut(s)	288
BpiI	GAAGAC	3 cut(s)	791, 1246, 1283
Bpu1102I	GCTNAGC	1 cut(s)	380
Bpu14I	TTCGAA	1 cut(s)	476
BpuEI	CTTGAG	2 cut(s)	314, 1119
BpuMI	CCSGG	3 cut(s)	524, 567, 634
BsaAI	YACGTR	1 cut(s)	511
BsaHI	GRCGYC	1 cut(s)	527
BsaJI	CCNNGG	7 cut(s)	357, 565, 654, 924, 1234, 1244, 1378
BsaWI	WCCGGW	2 cut(s)	857, 938
BsaXI	ACNNNNNCTCC	2 cut(s)	793, 823
Bsc4I	CCNNNNNNNGG	4 cut(s)	543, 627, 864, 925
Bse118I	RCCGGY	1 cut(s)	902
Bse1I	ACTGG	2 cut(s)	172, 1378
Bse3DI	GCAATG	2 cut(s)	870, 1470
BseBI	CCWGG	2 cut(s)	359, 1380
BseDI	CCNNGG	7 cut(s)	357, 565, 654, 924, 1234, 1244, 1378
BseGI	GGATG	3 cut(s)	312, 1330, 1385
BseLI	CCNNNNNNNGG	4 cut(s)	543, 627, 864, 925
BseMI	GCAATG	2 cut(s)	870, 1470
BseMII	CTCAG	3 cut(s)	122, 383, 394
BseNI	ACTGG	2 cut(s)	172, 1378
BseRI	GAGGAG	1 cut(s)	831
BseSI	GKGCMC	2 cut(s)	356, 689
BseXI	GCAGC	5 cut(s)	105, 262, 981, 1057, 1183
BseYI	CCCAGC	1 cut(s)	192
BsgI	GTGCAG	2 cut(s)	112, 988
Bsh1285I	CGRYCG	1 cut(s)	683
BshFI	GGCC	2 cut(s)	620, 1377
BshNI	GGYRCC	3 cut(s)	526, 686, 981
BsiEI	CGRYCG	1 cut(s)	683
BsiHKAI	GWGCWC	3 cut(s)	300, 356, 432
BsiHKCI	CYCGRG	2 cut(s)	401, 431
BsiSI	CCGG	6 cut(s)	524, 567, 633, 858, 903, 939
BslFI	GGGAC	2 cut(s)	296, 733
BslI	CCNNNNNNNGG	4 cut(s)	543, 627, 864, 925
BsmAI	GTCTC	2 cut(s)	398, 1337
BsmFI	GGGAC	2 cut(s)	296, 733
BsnI	GGCC	2 cut(s)	620, 1377
BsoBI	CYCGRG	2 cut(s)	401, 431
Bsp119I	TTCGAA	1 cut(s)	476
Bsp1286I	GDGCHC	4 cut(s)	300, 356, 432, 689
Bsp1407I	TGTACA	1 cut(s)	1102
Bsp143I	GATC	2 cut(s)	483, 946
Bsp1720I	GCTNAGC	1 cut(s)	380
Bsp19I	CCATGG	1 cut(s)	1244
BspACI	CCGC	5 cut(s)	530, 669, 679, 690, 1515
BspANI	GGCC	2 cut(s)	620, 1377
BspCNI	CTCAG	3 cut(s)	123, 382, 393
BspLI	GGNNCC	6 cut(s)	528, 581, 631, 688, 983, 1045
BspMAI	CTGCAG	1 cut(s)	588
BspOI	GCTAGC	1 cut(s)	200
BspT104I	TTCGAA	1 cut(s)	476
BspT107I	GGYRCC	3 cut(s)	526, 686, 981
BsrDI	GCAATG	2 cut(s)	870, 1470
BsrFI	RCCGGY	1 cut(s)	902
BsrGI	TGTACA	1 cut(s)	1102
BsrI	ACTGG	2 cut(s)	172, 1378
BssAI	RCCGGY	1 cut(s)	902
BssECI	CCNNGG	7 cut(s)	357, 565, 654, 924, 1234, 1244, 1378
BssMI	GATC	2 cut(s)	483, 946
BssNI	GRCGYC	1 cut(s)	527
BssSI	CACGAG	2 cut(s)	264, 876
BssT1I	CCWWGG	2 cut(s)	1234, 1244
Bst2BI	CACGAG	2 cut(s)	264, 876
Bst2UI	CCWGG	2 cut(s)	359, 1380
Bst4CI	ACNGT	8 cut(s)	334, 649, 847, 1048, 1091, 1126, 1184, 1459
BstACI	GRCGYC	1 cut(s)	527
BstAPI	GCANNNNNTGC	2 cut(s)	275, 351
BstAUI	TGTACA	1 cut(s)	1102
BstBAI	YACGTR	1 cut(s)	511
BstBI	TTCGAA	1 cut(s)	476
BstC8I	GCNNGC	4 cut(s)	10, 198, 219, 1494
BstDEI	CTNAG	4 cut(s)	131, 369, 380, 1214
BstDSI	CCRYGG	2 cut(s)	924, 1244
BstEII	GGTNACC	3 cut(s)	569, 649, 1415
BstF5I	GGATG	3 cut(s)	312, 1330, 1385
BstH2I	RGCGCY	2 cut(s)	530, 932
BstHHI	GCGC	2 cut(s)	529, 931
BstKTI	GATC	2 cut(s)	486, 949
BstMAI	GTCTC	2 cut(s)	398, 1337
BstMBI	GATC	2 cut(s)	483, 946
BstMCI	CGRYCG	1 cut(s)	683
BstMWI	GCNNNNNNNGC	4 cut(s)	218, 275, 351, 848
BstNI	CCWGG	2 cut(s)	359, 1380
BstNSI	RCATGY	2 cut(s)	221, 411
BstPAI	GACNNNNGTC	1 cut(s)	288
BstPI	GGTNACC	3 cut(s)	569, 649, 1415
BstSCI	CCNGG	5 cut(s)	357, 522, 565, 632, 1378
BstSFI	CTRYAG	3 cut(s)	584, 843, 1021
BstSLI	GKGCMC	2 cut(s)	356, 689
BstSNI	TACGTA	1 cut(s)	511
BstV1I	GCAGC	5 cut(s)	105, 262, 981, 1057, 1183
BstV2I	GAAGAC	3 cut(s)	791, 1246, 1283
BstX2I	RGATCY	1 cut(s)	946
BstYI	RGATCY	1 cut(s)	946
BsuRI	GGCC	2 cut(s)	620, 1377
BtgI	CCRYGG	2 cut(s)	924, 1244
BtsCI	GGATG	3 cut(s)	312, 1330, 1385
BtsI	GCAGTG	1 cut(s)	897
BtsIMutI	CAGTG	5 cut(s)	852, 897, 960, 1371, 1455
Cac8I	GCNNGC	4 cut(s)	10, 198, 219, 1494
CaiI	CAGNNNCTG	1 cut(s)	851
CfoI	GCGC	2 cut(s)	529, 931
Cfr10I	RCCGGY	1 cut(s)	902
Cfr13I	GGNCC	2 cut(s)	630, 1376
Csp6I	GTAC	5 cut(s)	512, 982, 1103, 1329, 1351
CviAII	CATG	9 cut(s)	87, 117, 214, 218, 279, 375, 408, 1245, 1421
CviQI	GTAC	5 cut(s)	512, 982, 1103, 1329, 1351
DdeI	CTNAG	4 cut(s)	131, 369, 380, 1214
DinI	GGCGCC	1 cut(s)	528
DpnI	GATC	2 cut(s)	485, 948
DpnII	GATC	2 cut(s)	483, 946
DraIII	CACNNNGTG	1 cut(s)	351
EaeI	YGGCCR	1 cut(s)	618
Ecl136II	GAGCTC	2 cut(s)	298, 430
Eco105I	TACGTA	1 cut(s)	511
Eco130I	CCWWGG	2 cut(s)	1234, 1244
Eco24I	GRGCYC	2 cut(s)	300, 432
Eco32I	GATATC	2 cut(s)	79, 186
Eco47I	GGWCC	1 cut(s)	630
Eco53kI	GAGCTC	2 cut(s)	298, 430
Eco57I	CTGAAG	1 cut(s)	1185
Eco88I	CYCGRG	2 cut(s)	401, 431
Eco91I	GGTNACC	3 cut(s)	569, 649, 1415
EcoICRI	GAGCTC	2 cut(s)	298, 430
EcoO65I	GGTNACC	3 cut(s)	569, 649, 1415
EcoRII	CCWGG	2 cut(s)	357, 1378
EcoRV	GATATC	2 cut(s)	79, 186
EcoT14I	CCWWGG	2 cut(s)	1234, 1244
EcoT22I	ATGCAT	1 cut(s)	219
EcoT38I	GRGCYC	2 cut(s)	300, 432
EgeI	GGCGCC	1 cut(s)	528
EheI	GGCGCC	1 cut(s)	528
ErhI	CCWWGG	2 cut(s)	1234, 1244
FaeI	CATG	9 cut(s)	90, 120, 217, 221, 282, 378, 411, 1248, 1424
FalI	AAGNNNNNCTT	2 cut(s)	956, 988
FaqI	GGGAC	2 cut(s)	296, 733
FatI	CATG	9 cut(s)	86, 116, 213, 217, 278, 374, 407, 1244, 1420
FbaI	TGATCA	1 cut(s)	483
FblI	GTMKAC	2 cut(s)	1002, 1050
Fnu4HI	GCNGC	7 cut(s)	6, 94, 276, 530, 970, 1071, 1197
FokI	GGATG	3 cut(s)	299, 1337, 1372
FriOI	GRGCYC	2 cut(s)	300, 432
Fsp4HI	GCNGC	7 cut(s)	6, 94, 276, 530, 970, 1071, 1197
FspBI	CTAG	7 cut(s)	197, 203, 242, 342, 1235, 1340, 1431
GlaI	GCGC	2 cut(s)	528, 930
GluI	GCNGC	7 cut(s)	6, 94, 276, 530, 970, 1071, 1197
GsaI	CCCAGC	1 cut(s)	196
HaeII	RGCGCY	2 cut(s)	530, 932
HaeIII	GGCC	2 cut(s)	620, 1377
HapII	CCGG	6 cut(s)	524, 567, 633, 858, 903, 939
HhaI	GCGC	2 cut(s)	529, 931
Hin1I	GRCGYC	1 cut(s)	527
Hin1II	CATG	9 cut(s)	90, 120, 217, 221, 282, 378, 411, 1248, 1424
Hin6I	GCGC	2 cut(s)	527, 929
HinP1I	GCGC	2 cut(s)	527, 929
HincII	GTYRAC	2 cut(s)	562, 1051
HindII	GTYRAC	2 cut(s)	562, 1051
HinfI	GANTC	2 cut(s)	607, 1345
HpaI	GTTAAC	1 cut(s)	562
HpaII	CCGG	6 cut(s)	524, 567, 633, 858, 903, 939
HphI	GGTGA	4 cut(s)	301, 661, 1370, 1504
Hpy166II	GTNNAC	7 cut(s)	337, 354, 562, 1003, 1051, 1105, 1455
Hpy188III	TCNNGA	8 cut(s)	181, 293, 316, 403, 739, 781, 878, 1260
Hpy8I	GTNNAC	7 cut(s)	337, 354, 562, 1003, 1051, 1105, 1455
Hpy99I	CGWCG	3 cut(s)	604, 740, 1052
HpyAV	CCTTC	5 cut(s)	410, 543, 570, 706, 841
HpyCH4III	ACNGT	8 cut(s)	334, 649, 847, 1048, 1091, 1126, 1184, 1459
HpyCH4IV	ACGT	4 cut(s)	471, 510, 735, 746
HpyCH4V	TGCA	7 cut(s)	93, 217, 278, 354, 586, 969, 1032
HpyF10VI	GCNNNNNNNGC	4 cut(s)	218, 275, 351, 848
HpyF3I	CTNAG	4 cut(s)	131, 369, 380, 1214
HpySE526I	ACGT	4 cut(s)	471, 510, 735, 746
Hsp92I	GRCGYC	1 cut(s)	527
Hsp92II	CATG	9 cut(s)	90, 120, 217, 221, 282, 378, 411, 1248, 1424
HspAI	GCGC	2 cut(s)	527, 929
KasI	GGCGCC	1 cut(s)	526
KpnI	GGTACC	1 cut(s)	985
Ksp22I	TGATCA	1 cut(s)	483
KspAI	GTTAAC	1 cut(s)	562
Kzo9I	GATC	2 cut(s)	483, 946
LmnI	GCTCC	5 cut(s)	427, 585, 801, 936, 1143
Lsp1109I	GCAGC	5 cut(s)	105, 262, 981, 1057, 1183
LweI	GCATC	2 cut(s)	680, 786
MaeI	CTAG	7 cut(s)	197, 203, 242, 342, 1235, 1340, 1431
MaeII	ACGT	4 cut(s)	471, 510, 735, 746
MalI	GATC	2 cut(s)	485, 948
MboI	GATC	2 cut(s)	483, 946
MboII	GAAGA	5 cut(s)	169, 796, 1251, 1288, 1428
MflI	RGATCY	1 cut(s)	946
MhlI	GDGCHC	4 cut(s)	300, 356, 432, 689
MlsI	TGGCCA	1 cut(s)	620
MluCI	AATT	5 cut(s)	478, 637, 835, 1016, 1266
MluNI	TGGCCA	1 cut(s)	620
Mly113I	GGCGCC	1 cut(s)	527
MlyI	GAGTC	1 cut(s)	1339
MmeI	TCCRAC	7 cut(s)	420, 575, 623, 793, 1032, 1121, 1296
Mox20I	TGGCCA	1 cut(s)	620
Mph1103I	ATGCAT	1 cut(s)	219
MscI	TGGCCA	1 cut(s)	620
MseI	TTAA	5 cut(s)	20, 504, 561, 1299, 1404
Msp20I	TGGCCA	1 cut(s)	620
MspA1I	CMGCKG	1 cut(s)	1517
MspI	CCGG	6 cut(s)	524, 567, 633, 858, 903, 939
MspR9I	CCNGG	5 cut(s)	359, 524, 567, 634, 1380
MvaI	CCWGG	2 cut(s)	359, 1380
MwoI	GCNNNNNNNGC	4 cut(s)	218, 275, 351, 848
NarI	GGCGCC	1 cut(s)	527
NciI	CCSGG	3 cut(s)	524, 567, 634
NcoI	CCATGG	1 cut(s)	1244
NdeII	GATC	2 cut(s)	483, 946
NheI	GCTAGC	1 cut(s)	196
NlaIII	CATG	9 cut(s)	90, 120, 217, 221, 282, 378, 411, 1248, 1424
NlaIV	GGNNCC	6 cut(s)	528, 581, 631, 688, 983, 1045
NmeAIII	GCCGAG	2 cut(s)	437, 1283
NmuCI	GTSAC	2 cut(s)	649, 878
NsiI	ATGCAT	1 cut(s)	219
NspI	RCATGY	2 cut(s)	221, 411
NspV	TTCGAA	1 cut(s)	476
PaeI	GCATGC	1 cut(s)	221
PaeR7I	CTCGAG	1 cut(s)	401
PfeI	GAWTC	1 cut(s)	607
PflMI	CCANNNNNTGG	1 cut(s)	925
PfoI	TCCNGGA	1 cut(s)	632
PkrI	GCNGC	7 cut(s)	7, 95, 277, 531, 971, 1072, 1198
PleI	GAGTC	1 cut(s)	1339
PluTI	GGCGCC	1 cut(s)	530
PpsI	GAGTC	1 cut(s)	1339
Ppu21I	YACGTR	1 cut(s)	511
PshAI	GACNNNNGTC	1 cut(s)	288
PsiI	TTATAA	1 cut(s)	1525
Psp124BI	GAGCTC	2 cut(s)	300, 432
Psp1406I	AACGTT	1 cut(s)	471
Psp6I	CCWGG	2 cut(s)	357, 1378
PspEI	GGTNACC	3 cut(s)	569, 649, 1415
PspFI	CCCAGC	1 cut(s)	192
PspGI	CCWGG	2 cut(s)	357, 1378
PspN4I	GGNNCC	6 cut(s)	528, 581, 631, 688, 983, 1045
PspPI	GGNCC	2 cut(s)	630, 1376
PstI	CTGCAG	1 cut(s)	588
PstNI	CAGNNNCTG	1 cut(s)	851
PsuI	RGATCY	1 cut(s)	946
RsaI	GTAC	5 cut(s)	513, 983, 1104, 1330, 1352
RsaNI	GTAC	5 cut(s)	512, 982, 1103, 1329, 1351
SacI	GAGCTC	2 cut(s)	300, 432
SalI	GTCGAC	1 cut(s)	1049
SaqAI	TTAA	5 cut(s)	20, 504, 561, 1299, 1404
SatI	GCNGC	7 cut(s)	6, 94, 276, 530, 970, 1071, 1197
Sau3AI	GATC	2 cut(s)	483, 946
Sau96I	GGNCC	2 cut(s)	630, 1376
SchI	GAGTC	1 cut(s)	1339
ScrFI	CCNGG	5 cut(s)	359, 524, 567, 634, 1380
SduI	GDGCHC	4 cut(s)	300, 356, 432, 689
SfaNI	GCATC	2 cut(s)	680, 786
SfcI	CTRYAG	3 cut(s)	584, 843, 1021
SfoI	GGCGCC	1 cut(s)	528
Sfr274I	CTCGAG	1 cut(s)	401
SfuI	TTCGAA	1 cut(s)	476
SinI	GGWCC	1 cut(s)	630
SlaI	CTCGAG	1 cut(s)	401
SmlI	CTYRAG	3 cut(s)	293, 401, 1134
SmoI	CTYRAG	3 cut(s)	293, 401, 1134
SnaBI	TACGTA	1 cut(s)	511
SpeI	ACTAGT	1 cut(s)	1430
SphI	GCATGC	1 cut(s)	221
Sse9I	AATT	5 cut(s)	478, 637, 835, 1016, 1266
SsiI	CCGC	5 cut(s)	530, 669, 679, 690, 1515
SspDI	GGCGCC	1 cut(s)	526
SspI	AATATT	2 cut(s)	757, 1036
SspMI	CTAG	7 cut(s)	197, 203, 242, 342, 1235, 1340, 1431
SstI	GAGCTC	2 cut(s)	300, 432
StyD4I	CCNGG	5 cut(s)	357, 522, 565, 632, 1378
StyI	CCWWGG	2 cut(s)	1234, 1244
TaaI	ACNGT	8 cut(s)	334, 649, 847, 1048, 1091, 1126, 1184, 1459
TaiI	ACGT	4 cut(s)	474, 513, 738, 749
TaqI	TCGA	6 cut(s)	402, 464, 476, 674, 1050, 1261
TaqII	GACCGA	2 cut(s)	222, 668
TasI	AATT	5 cut(s)	478, 637, 835, 1016, 1266
TatI	WGTACW	2 cut(s)	1102, 1350
TauI	GCSGC	1 cut(s)	532
TfiI	GAWTC	1 cut(s)	607
Tru1I	TTAA	5 cut(s)	20, 504, 561, 1299, 1404
Tru9I	TTAA	5 cut(s)	20, 504, 561, 1299, 1404
TscAI	CASTG	5 cut(s)	852, 904, 960, 1378, 1462
TseFI	GTSAC	2 cut(s)	649, 878
TseI	GCWGC	6 cut(s)	5, 93, 275, 969, 1070, 1196
Tsp45I	GTSAC	2 cut(s)	649, 878
TspDTI	ATGAA	7 cut(s)	859, 1045, 1166, 1289, 1374, 1382, 1459
TspGWI	ACGGA	1 cut(s)	923
TspRI	CASTG	5 cut(s)	852, 904, 960, 1378, 1462
Van91I	CCANNNNNTGG	1 cut(s)	925
VneI	GTGCAC	1 cut(s)	352
VpaK11BI	GGWCC	1 cut(s)	630
XapI	RAATTY	1 cut(s)	478
XceI	RCATGY	2 cut(s)	221, 411
XhoI	CTCGAG	1 cut(s)	401
XmaJI	CCTAGG	1 cut(s)	1234
XmiI	GTMKAC	2 cut(s)	1002, 1050
XspI	CTAG	7 cut(s)	197, 203, 242, 342, 1235, 1340, 1431
Zsp2I	ATGCAT	1 cut(s)	219

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.