Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0004889

Physical Location & Seq

Genomic Coordinates Forward (+)

28023294 .. 28025283

Gene Structure 1990 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1195500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1557 bp

ATGGCGACAAATCTAAGTTCATTCGTCCTATGTGTCTCCTTTGTTTTATTCTTCTCACCTGCCCTTTCAAACATTCTCCACAGCAGCATAGATTCATGGTGCAAAAAAACCCCCTACCCTGAAACTTGCAAATACACCATGACCCAAGACGAAAAATCTCTCTCTATCCCCAAACAACTATCCAACTTCAAGAAACTTGCAGTCCAAGTAACATTAGAAAGAGCACTCAAAGCGCAAAGTCACAACAAGTGGCTAGGGCAAAAGTGTAGAAACAAGAAAGAAAGAGCTGCATGGGCTGACTGCATGAGCCTCTATGAGGAAACCATCAAACTCCTCAACCAAACCATAGACCCTGCCACAAAATCCACCGACTATGACACGCAAACTTGGCTCAGCACTGCTCTCACGAACCTTGACACGTGTCGGGCTGGGTTCGTGGAGCTTGAGGTTTCGGACTTTGTGTTTCCGCTCATGTCCAACAATGTGTCCAAGCTGATAAGTAACACTTTGTCACTTTGGAATTCCTCAAATGTTTCACCGGTGACAAATATGTACACAGAGGGTTATCCTAGTTGGGTTTCCCCTGGGGATAGGAAACTGTTGCAGTCGACGCCCACAGCGGACGTAGTGGTCGCTCAAGACGGGTCGGGGAACTATAAGACGATCAAAGCCGCTTTGGATGCTGCGGCCGCAAGGGGTGGGAGTAAGAGGTTTGTGATACATGTGAAGAGAGGGGTTTATAAGGAAAACCTTGAGATTAAGTTGAAGAACATTTATCTGCTGGGTGATGGACTGAGGTATACTATTATCACCGGCAGCCGTAGTGTTGTTGGAGGTTCTACAACTTTCAAGTCTGCAACTGTTGCGGTAACTGGTGAAGGCTTCATTGCTCGTGGCATAACATTCCGCAACACAGCTGGCCCAGAGAACCATCAAGCAGTAGCCCTCAGATCAGGCGCCGACCTCTCAGTTTTCTACCGCTGCGGATTCGAAGGCTACCAAGATACCCTCTACACCCACTCCCAACGACAGTTCTACAGAGAATGCTATGTCTACGGCACCGTTGATTTTATCTTTGGCAACGCTGCGGTTGTTTTCCAAAACTGCATGATCTACGCAAGAAAACCCATGAGTGGTCAACAGAACACAATCACAGCCCAAGGCCGAACGGACCCGAACCAGAACACGGGTATTTCTATCCACGACTCACGTGTCATGGCCGCACTGGACCTTGAATCGTCCGTCGGCTCAGTCAAGACATTCTTGGGTAGACCATGGAAGGAGTATTCACGCACAGTTTTTATGCAATCTTTTTTAGACACCCTGGTTGACCCAGCTGGGTGGCTCCAGTGGAGCGGTGATTTTGCTTTGAAGACATTGTATTATGGAGAGTACAAGAACATTGGACCGGGTGCACCTACTACTGGCAGGGTCAAGTGGGGTGGCTATCGAATTATAACTAGTTCATCGGAAGCATCGAAGTTTACTGTGGATAATTTCATTGCTGGAAGCTCATGGTTGCCTGCTACAAATGTACCATTCACGGCTGGCCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0016020 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0042742 GO:0043086 GO:0043207 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0050829 GO:0050896 GO:0051704 GO:0051707 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098542 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

519

Amino Acids

57.29

Weight (kDa)

9.1

Isoelectric Point (pI)

34.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	29 - 172	4.7e-31	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	208 - 503	5.2e-142	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004889)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G45220
fragaria_vesca	FvH4_7g11320 FvH4_7g27760
malus_domestica	MD02G1207900.v1.1 MD07G1255000.v1.1 MD11G1195500.v1.1
prunus_persica	Prupe.2G141000_v2.0.a1 Prupe.2G278200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0349521 RchiOBHm_Chr1g0374551
rosa_laevigata	RLG00000026729 RLG00000028541 RLG00000028544
rosa_multiflora	Rmu_sc0002495.1_g000016 Rmu_sc0002495.1_g000029 Rmu_sc0004947.1_g000001
rosa_roxburghii	Rroxscaffold_4G00283410 Rroxscaffold_4G00305490
rosa_rugosa	Rorug01G0204300 Rorug01G0384100
rosa_samantha	Rh1AG394800 Rh1BG187700 Rh1BG359500 Rh1CG206500 Rh1CG372600 Rh1DG217200 Rh1DG390100
rosa_wichuraiana	Rw1G018840 Rw1G018870 Rw1G035010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	741, 1457
AarI	CACCTGC	1 cut(s)	67
AasI	GACNNNNNNGTC	2 cut(s)	629, 1211
Acc36I	ACCTGC	1 cut(s)	67
AccB1I	GGYRCC	2 cut(s)	956, 1058
AccBSI	CCGCTC	2 cut(s)	469, 1356
AccI	GTMKAC	4 cut(s)	608, 800, 1053, 1270
AcoI	YGGCCR	2 cut(s)	687, 1218
AcsI	RAATTY	1 cut(s)	520
AcvI	CACGTG	2 cut(s)	420, 1211
AcyI	GRCGYC	2 cut(s)	611, 957
AfaI	GTAC	3 cut(s)	554, 1394, 1536
AfiI	CCNNNNNNNGG	4 cut(s)	316, 1133, 1186, 1424
AflIII	ACRYGT	4 cut(s)	417, 419, 721, 1210
AgeI	ACCGGT	1 cut(s)	538
AgsI	TTSAA	6 cut(s)	69, 190, 766, 850, 1235, 1372
AhlI	ACTAGT	1 cut(s)	1460
AjnI	CCWGG	2 cut(s)	583, 1323
AluBI	AGCT	6 cut(s)	287, 442, 493, 917, 1337, 1512
AluI	AGCT	6 cut(s)	287, 442, 493, 917, 1337, 1512
Alw21I	GWGCWC	2 cut(s)	226, 1417
Alw26I	GTCTC	1 cut(s)	40
Alw44I	GTGCAC	1 cut(s)	1413
AoxI	GGCC	5 cut(s)	687, 919, 1162, 1218, 1549
ApaLI	GTGCAC	1 cut(s)	1413
ApeKI	GCWGC	6 cut(s)	84, 287, 683, 816, 981, 1085
ApoI	RAATTY	1 cut(s)	520
AsiGI	ACCGGT	1 cut(s)	538
AspLEI	GCGC	2 cut(s)	235, 959
AspS9I	GGNCC	4 cut(s)	920, 1171, 1228, 1406
AsuC2I	CCSGG	1 cut(s)	1410
AsuHPI	GGTGA	7 cut(s)	48, 528, 553, 797, 802, 887, 1370
AsuII	TTCGAA	1 cut(s)	990
AvaII	GGWCC	3 cut(s)	1171, 1228, 1406
BaeGI	GKGCMC	1 cut(s)	1417
BaeI	ACNNNNGTAYC	2 cut(s)	1518, 1551
BanI	GGYRCC	2 cut(s)	956, 1058
BauI	CACGAG	1 cut(s)	891
BbrPI	CACGTG	2 cut(s)	420, 1211
BbsI	GAAGAC	1 cut(s)	1379
Bbv12I	GWGCWC	2 cut(s)	226, 1417
BbvI	GCAGC	6 cut(s)	96, 274, 670, 828, 968, 1072
BccI	CCATC	3 cut(s)	332, 782, 939
BceAI	ACGGC	2 cut(s)	804, 1072
BciT130I	CCWGG	2 cut(s)	585, 1325
BcnI	CCSGG	1 cut(s)	1410
BcoDI	GTCTC	1 cut(s)	40
BcuI	ACTAGT	1 cut(s)	1460
BfaI	CTAG	3 cut(s)	254, 570, 1461
BfmI	CTRYAG	1 cut(s)	1036
BfoI	RGCGCY	1 cut(s)	960
BfuAI	ACCTGC	1 cut(s)	67
BlpI	GCTNAGC	1 cut(s)	392
Bme1390I	CCNGG	3 cut(s)	585, 1325, 1410
Bme18I	GGWCC	3 cut(s)	1171, 1228, 1406
BmgT120I	GGNCC	4 cut(s)	920, 1171, 1228, 1406
BmiI	GGNNCC	4 cut(s)	958, 1060, 1173, 1346
BmrFI	CCNGG	3 cut(s)	585, 1325, 1410
BmsI	GCATC	2 cut(s)	670, 1484
BoxI	GACNNNNGTC	1 cut(s)	420
BpiI	GAAGAC	1 cut(s)	1379
BpmI	CTGGAG	1 cut(s)	1331
Bpu1102I	GCTNAGC	1 cut(s)	392
Bpu14I	TTCGAA	1 cut(s)	990
BpuEI	CTTGAG	3 cut(s)	464, 621, 773
BpuMI	CCSGG	1 cut(s)	1410
BsaAI	YACGTR	2 cut(s)	420, 1211
BsaHI	GRCGYC	2 cut(s)	611, 957
BsaJI	CCNNGG	5 cut(s)	583, 584, 1159, 1274, 1323
BsaWI	WCCGGW	1 cut(s)	538
BsaXI	ACNNNNNCTCC	2 cut(s)	1004, 1034
Bsc4I	CCNNNNNNNGG	4 cut(s)	316, 1133, 1186, 1424
Bse118I	RCCGGY	2 cut(s)	538, 812
Bse1I	ACTGG	4 cut(s)	877, 1230, 1348, 1429
Bse3DI	GCAATG	2 cut(s)	885, 1500
BseBI	CCWGG	2 cut(s)	585, 1325
BseDI	CCNNGG	5 cut(s)	583, 584, 1159, 1274, 1323
BseGI	GGATG	1 cut(s)	685
BseLI	CCNNNNNNNGG	4 cut(s)	316, 1133, 1186, 1424
BseMI	GCAATG	2 cut(s)	885, 1500
BseMII	CTCAG	5 cut(s)	406, 785, 961, 981, 1263
BseNI	ACTGG	4 cut(s)	877, 1230, 1348, 1429
BseRI	GAGGAG	1 cut(s)	323
BseSI	GKGCMC	1 cut(s)	1417
BseX3I	CGGCCG	1 cut(s)	687
BseXI	GCAGC	6 cut(s)	96, 274, 670, 828, 968, 1072
BseYI	CCCAGC	4 cut(s)	428, 781, 1333, 1337
Bsh1285I	CGRYCG	1 cut(s)	690
BshFI	GGCC	5 cut(s)	689, 921, 1164, 1220, 1551
BshNI	GGYRCC	2 cut(s)	956, 1058
BshTI	ACCGGT	1 cut(s)	538
BsiEI	CGRYCG	1 cut(s)	690
BsiHKAI	GWGCWC	2 cut(s)	226, 1417
BsiSI	CCGG	3 cut(s)	539, 813, 1409
BslI	CCNNNNNNNGG	4 cut(s)	316, 1133, 1186, 1424
BsmAI	GTCTC	1 cut(s)	40
BsmI	GAATGC	1 cut(s)	1049
BsnI	GGCC	5 cut(s)	689, 921, 1164, 1220, 1551
Bsp119I	TTCGAA	1 cut(s)	990
Bsp1286I	GDGCHC	2 cut(s)	226, 1417
Bsp1407I	TGTACA	1 cut(s)	552
Bsp143I	GATC	3 cut(s)	663, 950, 1110
Bsp1720I	GCTNAGC	1 cut(s)	392
Bsp19I	CCATGG	1 cut(s)	1274
BspANI	GGCC	5 cut(s)	689, 921, 1164, 1220, 1551
BspCNI	CTCAG	5 cut(s)	405, 786, 960, 980, 1262
BspLI	GGNNCC	4 cut(s)	958, 1060, 1173, 1346
BspMI	ACCTGC	1 cut(s)	67
BspT104I	TTCGAA	1 cut(s)	990
BspT107I	GGYRCC	2 cut(s)	956, 1058
BsrBI	CCGCTC	2 cut(s)	469, 1356
BsrDI	GCAATG	2 cut(s)	885, 1500
BsrFI	RCCGGY	2 cut(s)	538, 812
BsrGI	TGTACA	1 cut(s)	552
BsrI	ACTGG	4 cut(s)	877, 1230, 1348, 1429
BssAI	RCCGGY	2 cut(s)	538, 812
BssECI	CCNNGG	5 cut(s)	583, 584, 1159, 1274, 1323
BssMI	GATC	3 cut(s)	663, 950, 1110
BssNAI	GTATAC	1 cut(s)	801
BssNI	GRCGYC	2 cut(s)	611, 957
BssSI	CACGAG	1 cut(s)	891
BssT1I	CCWWGG	2 cut(s)	1159, 1274
Bst1107I	GTATAC	1 cut(s)	801
Bst2BI	CACGAG	1 cut(s)	891
Bst2UI	CCWGG	2 cut(s)	585, 1325
Bst4CI	ACNGT	6 cut(s)	600, 862, 1032, 1063, 1297, 1489
Bst6I	CTCTTC	1 cut(s)	722
BstACI	GRCGYC	2 cut(s)	611, 957
BstAPI	GCANNNNNTGC	1 cut(s)	863
BstAUI	TGTACA	1 cut(s)	552
BstBAI	YACGTR	2 cut(s)	420, 1211
BstBI	TTCGAA	1 cut(s)	990
BstC8I	GCNNGC	3 cut(s)	919, 1524, 1549
BstDEI	CTNAG	6 cut(s)	14, 392, 794, 947, 967, 1249
BstDSI	CCRYGG	1 cut(s)	1274
BstENI	CCTNNNNNAGG	1 cut(s)	314
BstF5I	GGATG	1 cut(s)	685
BstH2I	RGCGCY	1 cut(s)	960
BstHHI	GCGC	2 cut(s)	235, 959
BstKTI	GATC	3 cut(s)	666, 953, 1113
BstMAI	GTCTC	1 cut(s)	40
BstMBI	GATC	3 cut(s)	663, 950, 1110
BstMCI	CGRYCG	1 cut(s)	690
BstMWI	GCNNNNNNNGC	7 cut(s)	230, 293, 388, 610, 680, 689, 863
BstNI	CCWGG	2 cut(s)	585, 1325
BstNSI	RCATGY	1 cut(s)	725
BstPAI	GACNNNNGTC	1 cut(s)	420
BstSCI	CCNGG	3 cut(s)	583, 1323, 1408
BstSFI	CTRYAG	1 cut(s)	1036
BstSLI	GKGCMC	1 cut(s)	1417
BstV1I	GCAGC	6 cut(s)	96, 274, 670, 828, 968, 1072
BstV2I	GAAGAC	1 cut(s)	1379
BstXI	CCANNNNNNTGG	1 cut(s)	1341
BstZ17I	GTATAC	1 cut(s)	801
BstZI	CGGCCG	1 cut(s)	687
BsuRI	GGCC	5 cut(s)	689, 921, 1164, 1220, 1551
BtgI	CCRYGG	1 cut(s)	1274
BtsCI	GGATG	1 cut(s)	685
BtsI	GCAGTG	1 cut(s)	396
BtsIMutI	CAGTG	3 cut(s)	396, 1223, 1355
BveI	ACCTGC	1 cut(s)	67
Cac8I	GCNNGC	3 cut(s)	919, 1524, 1549
CciNI	GCGGCCGC	1 cut(s)	687
CfoI	GCGC	2 cut(s)	235, 959
Cfr10I	RCCGGY	2 cut(s)	538, 812
Cfr13I	GGNCC	4 cut(s)	920, 1171, 1228, 1406
CseI	GACGC	1 cut(s)	619
Csp6I	GTAC	3 cut(s)	553, 1393, 1535
CspAI	ACCGGT	1 cut(s)	538
CspCI	CAANNNNNGTGG	2 cut(s)	1423, 1458
CviQI	GTAC	3 cut(s)	553, 1393, 1535
DdeI	CTNAG	6 cut(s)	14, 392, 794, 947, 967, 1249
DinI	GGCGCC	1 cut(s)	958
DpnI	GATC	3 cut(s)	665, 952, 1112
DpnII	GATC	3 cut(s)	663, 950, 1110
DrdI	GACNNNNNNGTC	2 cut(s)	629, 1211
DseDI	GACNNNNNNGTC	2 cut(s)	629, 1211
EaeI	YGGCCR	2 cut(s)	687, 1218
EagI	CGGCCG	1 cut(s)	687
Eam1104I	CTCTTC	1 cut(s)	722
EarI	CTCTTC	1 cut(s)	722
EclXI	CGGCCG	1 cut(s)	687
Eco130I	CCWWGG	2 cut(s)	1159, 1274
Eco47I	GGWCC	3 cut(s)	1171, 1228, 1406
Eco52I	CGGCCG	1 cut(s)	687
Eco72I	CACGTG	2 cut(s)	420, 1211
EcoNI	CCTNNNNNAGG	1 cut(s)	314
EcoRI	GAATTC	1 cut(s)	520
EcoRII	CCWGG	2 cut(s)	583, 1323
EcoT14I	CCWWGG	2 cut(s)	1159, 1274
EgeI	GGCGCC	1 cut(s)	958
EheI	GGCGCC	1 cut(s)	958
ErhI	CCWWGG	2 cut(s)	1159, 1274
FalI	AAGNNNNNCTT	4 cut(s)	490, 522, 1247, 1279
FblI	GTMKAC	4 cut(s)	608, 800, 1053, 1270
FokI	GGATG	1 cut(s)	692
FspBI	CTAG	3 cut(s)	254, 570, 1461
GlaI	GCGC	2 cut(s)	234, 958
GsaI	CCCAGC	4 cut(s)	432, 785, 1337, 1341
GsuI	CTGGAG	1 cut(s)	1331
HaeII	RGCGCY	1 cut(s)	960
HaeIII	GGCC	5 cut(s)	689, 921, 1164, 1220, 1551
HapII	CCGG	3 cut(s)	539, 813, 1409
HgaI	GACGC	1 cut(s)	619
HhaI	GCGC	2 cut(s)	235, 959
Hin1I	GRCGYC	2 cut(s)	611, 957
Hin6I	GCGC	2 cut(s)	233, 957
HinP1I	GCGC	2 cut(s)	233, 957
HincII	GTYRAC	3 cut(s)	609, 1139, 1330
HindII	GTYRAC	3 cut(s)	609, 1139, 1330
HinfI	GANTC	4 cut(s)	92, 987, 1205, 1235
HpaII	CCGG	3 cut(s)	539, 813, 1409
HphI	GGTGA	7 cut(s)	48, 528, 553, 797, 802, 887, 1370
Hpy166II	GTNNAC	9 cut(s)	555, 609, 801, 1054, 1139, 1271, 1330, 1415, 1485
Hpy188I	TCNGA	3 cut(s)	454, 950, 1471
Hpy188III	TCNNGA	4 cut(s)	190, 406, 638, 1255
Hpy8I	GTNNAC	9 cut(s)	555, 609, 801, 1054, 1139, 1271, 1330, 1415, 1485
Hpy99I	CGWCG	2 cut(s)	613, 1247
HpyAV	CCTTC	3 cut(s)	872, 986, 1273
HpyCH4III	ACNGT	6 cut(s)	600, 862, 1032, 1063, 1297, 1489
HpyCH4IV	ACGT	3 cut(s)	419, 624, 1210
HpyF10VI	GCNNNNNNNGC	7 cut(s)	230, 293, 388, 610, 680, 689, 863
HpyF3I	CTNAG	6 cut(s)	14, 392, 794, 947, 967, 1249
HpySE526I	ACGT	3 cut(s)	419, 624, 1210
Hsp92I	GRCGYC	2 cut(s)	611, 957
HspAI	GCGC	2 cut(s)	233, 957
KasI	GGCGCC	1 cut(s)	956
Kzo9I	GATC	3 cut(s)	663, 950, 1110
LmnI	GCTCC	3 cut(s)	439, 1350, 1353
Lsp1109I	GCAGC	6 cut(s)	96, 274, 670, 828, 968, 1072
LweI	GCATC	2 cut(s)	670, 1484
MaeI	CTAG	3 cut(s)	254, 570, 1461
MaeII	ACGT	3 cut(s)	419, 624, 1210
MaeIII	GTNAC	6 cut(s)	208, 239, 500, 510, 541, 868
MalI	GATC	3 cut(s)	665, 952, 1112
MbiI	CCGCTC	2 cut(s)	469, 1356
MboI	GATC	3 cut(s)	663, 950, 1110
MboII	GAAGA	4 cut(s)	43, 739, 778, 1384
MhlI	GDGCHC	2 cut(s)	226, 1417
MluCI	AATT	3 cut(s)	520, 1452, 1495
Mly113I	GGCGCC	1 cut(s)	957
MlyI	GAGTC	1 cut(s)	1199
MmeI	TCCRAC	3 cut(s)	207, 501, 811
MseI	TTAA	1 cut(s)	759
MspA1I	CMGCKG	4 cut(s)	620, 917, 981, 1337
MspI	CCGG	3 cut(s)	539, 813, 1409
MspR9I	CCNGG	3 cut(s)	585, 1325, 1410
Mva1269I	GAATGC	1 cut(s)	1049
MvaI	CCWGG	2 cut(s)	585, 1325
MwoI	GCNNNNNNNGC	7 cut(s)	230, 293, 388, 610, 680, 689, 863
NarI	GGCGCC	1 cut(s)	957
NciI	CCSGG	1 cut(s)	1410
NcoI	CCATGG	1 cut(s)	1274
NdeII	GATC	3 cut(s)	663, 950, 1110
NlaIV	GGNNCC	4 cut(s)	958, 1060, 1173, 1346
NmuCI	GTSAC	3 cut(s)	239, 510, 541
NotI	GCGGCCGC	1 cut(s)	687
NspI	RCATGY	1 cut(s)	725
NspV	TTCGAA	1 cut(s)	990
PaqCI	CACCTGC	1 cut(s)	67
PasI	CCCWGGG	1 cut(s)	584
PciI	ACATGT	1 cut(s)	721
PcsI	WCGNNNNNNNCGW	1 cut(s)	1475
PctI	GAATGC	1 cut(s)	1049
PfeI	GAWTC	3 cut(s)	92, 987, 1235
PinAI	ACCGGT	1 cut(s)	538
PleI	GAGTC	1 cut(s)	1199
PluTI	GGCGCC	1 cut(s)	960
PmaCI	CACGTG	2 cut(s)	420, 1211
PmlI	CACGTG	2 cut(s)	420, 1211
PpsI	GAGTC	1 cut(s)	1199
Ppu21I	YACGTR	2 cut(s)	420, 1211
PscI	ACATGT	1 cut(s)	721
PshAI	GACNNNNGTC	1 cut(s)	420
PsiI	TTATAA	2 cut(s)	741, 1457
Psp6I	CCWGG	2 cut(s)	583, 1323
PspCI	CACGTG	2 cut(s)	420, 1211
PspFI	CCCAGC	4 cut(s)	428, 781, 1333, 1337
PspGI	CCWGG	2 cut(s)	583, 1323
PspN4I	GGNNCC	4 cut(s)	958, 1060, 1173, 1346
PspPI	GGNCC	4 cut(s)	920, 1171, 1228, 1406
PvuII	CAGCTG	2 cut(s)	917, 1337
RsaI	GTAC	3 cut(s)	554, 1394, 1536
RsaNI	GTAC	3 cut(s)	553, 1393, 1535
SalI	GTCGAC	1 cut(s)	607
SaqAI	TTAA	1 cut(s)	759
Sau3AI	GATC	3 cut(s)	663, 950, 1110
Sau96I	GGNCC	4 cut(s)	920, 1171, 1228, 1406
SchI	GAGTC	1 cut(s)	1199
ScrFI	CCNGG	3 cut(s)	585, 1325, 1410
SduI	GDGCHC	2 cut(s)	226, 1417
SfaNI	GCATC	2 cut(s)	670, 1484
SfcI	CTRYAG	1 cut(s)	1036
SfoI	GGCGCC	1 cut(s)	958
SfuI	TTCGAA	1 cut(s)	990
SgrAI	CRCCGGYG	1 cut(s)	538
SinI	GGWCC	3 cut(s)	1171, 1228, 1406
SmlI	CTYRAG	3 cut(s)	443, 636, 752
SmoI	CTYRAG	3 cut(s)	443, 636, 752
SpeI	ACTAGT	1 cut(s)	1460
Sse9I	AATT	3 cut(s)	520, 1452, 1495
SspDI	GGCGCC	1 cut(s)	956
SspMI	CTAG	3 cut(s)	254, 570, 1461
StyD4I	CCNGG	3 cut(s)	583, 1323, 1408
StyI	CCWWGG	2 cut(s)	1159, 1274
TaaI	ACNGT	6 cut(s)	600, 862, 1032, 1063, 1297, 1489
TaiI	ACGT	3 cut(s)	422, 627, 1213
TaqI	TCGA	4 cut(s)	608, 990, 1450, 1478
TasI	AATT	3 cut(s)	520, 1452, 1495
TatI	WGTACW	2 cut(s)	552, 1392
TauI	GCSGC	4 cut(s)	674, 689, 692, 1223
TfiI	GAWTC	3 cut(s)	92, 987, 1235
Tru1I	TTAA	1 cut(s)	759
Tru9I	TTAA	1 cut(s)	759
TscAI	CASTG	3 cut(s)	403, 1230, 1355
TseFI	GTSAC	3 cut(s)	239, 510, 541
TseI	GCWGC	6 cut(s)	84, 287, 683, 816, 981, 1085
Tsp45I	GTSAC	3 cut(s)	239, 510, 541
TspDTI	ATGAA	5 cut(s)	9, 84, 874, 1455, 1489
TspGWI	ACGGA	2 cut(s)	1184, 1231
TspRI	CASTG	3 cut(s)	403, 1230, 1355
VneI	GTGCAC	1 cut(s)	1413
VpaK11BI	GGWCC	3 cut(s)	1171, 1228, 1406
XagI	CCTNNNNNAGG	1 cut(s)	314
XapI	RAATTY	1 cut(s)	520
XceI	RCATGY	1 cut(s)	725
XmiI	GTMKAC	4 cut(s)	608, 800, 1053, 1270
XspI	CTAG	3 cut(s)	254, 570, 1461

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.