Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1C

Orthogroup ID

OG0004889

Physical Location & Seq

Genomic Coordinates Reverse (-)

44770744 .. 44785031

Gene Structure 14288 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1CG206500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1761 bp

ATGGCATCTCGGTTTTTCTTAACACTTTCTTTCATGCTTTATCTTTCTCCATTCGTACTTGGTTACTCTGTCAATGATCTCAAAACATGGTGCAGCCAAACCCCAAACCCACAACCATGTGAGGATCTTCTGAGCCATAACAAAAATCAAATCCCCATTAAACAAAAATCCCAGTTCTTCCAGATATCTACCCAGCTAGCCCTAGACCGGGCCATGCATGCTCAAACCAACTTCCTTTTGTTGGGCTCAAAGTTTGAAAATCCACGTGCAAAAGCTGCATGGGACGATTGTCTAGAGCTCTATGACCACACAATCCACAAGCTCAACACAACCATAAACCCTAGCACCAAGTGCACCCAGGAGGATGCTCAGACATGGCTCAGCACCGCTCTCACAAACCTCCAGACATGCCGAGAAGGGTTTTCCGAGCTCGGGGTGTCGGATTTCTTTCTGCCTGCGATGTCGAATAACGTTTCTAATTTGATCAGCAACACCCTTTCCATTAACTACGTACCGTACAACCCTCAGCCCGCTTACAAAGACGGGTTTCCAACTTGGGTTAACCCCGGGAACCGGAAGCTCTTGCAGTCAACTTCTCCGAAGGCGAATCTCGTGGTGGCCAAAGACGGGTCGGGGAATTACAAGACGGTGGCCGAGGCAATAACTGCCGCGTCGAAGCGATCAGGCACCGCAAGGTATGTGATATATGTGAAGGCAGGAACATACAAGGAGAACGTTGTGATAAAGTCAACGAATATTATGTTGATGGGAGATGGCATAGGGAAGACTATTATCACAGGAAGCAAAAGTGTGGGAGGAGGTTCTACAACTTTCAATTCAGCTACAGTAGCTGTAACCGGAGATGGGTTTATTGCTCGTGACATTACATTCCAAAACACTGCCGGTGTATCAAACAACCAAGCAGTGGCACTCCGGTCCGGTTCAGATCTTTCAGTGTTCTACAAATGCAGCTTTGAGGGGTACCAAGACACCCTCTATGTCTACTCCGACAGGCAATTCTACAAAGAGTGCAACATTTATGGAACTGTAGACTTCATTTTTGGCAATGCTGCTGTGGTTTTACAAAATTGTAATATTTATGTACGCAACCACAAAACCAACACCATTACTGCTCAAGGTCGGAGCGACCCTAACCAGAACACAGGGATTTCCATTCATAACTGTAAGGTTATGGCTGCTTCGGATTTAGTCCTAAGTTCAACCAAAACGTACCTAGGAAGACCATGGAGGGAGTATTCTCGAACGGTTTTCATGAAGACCTACCTTGATAGTTTGATTAACTCGGCTGGTTGGTTGGAATGGAGTGGTACTTTTGCCCTAAAGACTCTGTACTATGGTGAGTATATGAATACTGGCCCCGGTTCATCTACTTCAGGTAGGGTTAAGTGGGCAGGTTACCATGTCATAACTAGTTCTTCGGAGGCTTCCAAGTTCACTGTTAATAGCTTCATTGCTGGTAGTTCTTGGTTGCCAGCCACCAATGTGCCGTTTACGGCTGGAGCTGAAACTCTTGCCTTTTCTCTACTTGACGAAGCAGGTAAAGTAAGGCGGATTTATGATATTGCAAATGTTTCGTCCTCCATGAATCTTATTGAAAAGACCCACACAGCAGATACAAGGAAGCCTGCATATAAATGGTTGGGATTGAGAGGAAAAGATGCTACGAGTTCAACTTCAGACGAGTCTAGAAAAAGAGCCTTTGGAACAGATATCACAAACGTTGTACGAGGGGCAAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

586

Amino Acids

64.46

Weight (kDa)

9.16

Isoelectric Point (pI)

33.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	26 - 168	1.9e-30	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	203 - 493	1.7e-137	Pectinesterase
WHD_E2F_TDP	PF02319	521 - 555	1.7e-07	E2F/DP family winged-helix DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004889)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G45220
fragaria_vesca	FvH4_7g11320 FvH4_7g27760
malus_domestica	MD02G1207900.v1.1 MD07G1255000.v1.1 MD11G1195500.v1.1
prunus_persica	Prupe.2G141000_v2.0.a1 Prupe.2G278200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0349521 RchiOBHm_Chr1g0374551
rosa_laevigata	RLG00000026729 RLG00000028541 RLG00000028544
rosa_multiflora	Rmu_sc0002495.1_g000016 Rmu_sc0002495.1_g000029 Rmu_sc0004947.1_g000001
rosa_roxburghii	Rroxscaffold_4G00283410 Rroxscaffold_4G00305490
rosa_rugosa	Rorug01G0204300 Rorug01G0384100
rosa_samantha	Rh1AG394800 Rh1BG187700 Rh1BG359500 Rh1CG206500 Rh1CG372600 Rh1DG217200 Rh1DG390100
rosa_wichuraiana	Rw1G018840 Rw1G018870 Rw1G035010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1403, 1547
Acc65I	GGTACC	1 cut(s)	981
AccB1I	GGYRCC	2 cut(s)	686, 981
AccB7I	CCANNNNNTGG	1 cut(s)	925
AccBSI	CCGCTC	1 cut(s)	389
AccI	GTMKAC	2 cut(s)	1002, 1050
AccII	CGCG	1 cut(s)	671
AciI	CCGC	5 cut(s)	387, 531, 669, 690, 1570
AclI	AACGTT	3 cut(s)	471, 735, 1740
AclWI	GGATC	1 cut(s)	132
AcoI	YGGCCR	2 cut(s)	618, 651
AcuI	CTGAAG	2 cut(s)	1377, 1680
AcvI	CACGTG	1 cut(s)	266
AdeI	CACNNNGTG	1 cut(s)	351
AfaI	GTAC	9 cut(s)	57, 513, 518, 983, 1104, 1232, 1330, 1352, 1746
AfiI	CCNNNNNNNGG	8 cut(s)	207, 208, 241, 432, 573, 627, 864, 925
AgsI	TTSAA	5 cut(s)	257, 835, 1221, 1616, 1692
AhlI	ACTAGT	1 cut(s)	1430
AjnI	CCWGG	1 cut(s)	357
AleI	CACNNNNGTG	1 cut(s)	1502
Alw21I	GWGCWC	3 cut(s)	300, 356, 432
Alw44I	GTGCAC	1 cut(s)	352
AlwI	GGATC	1 cut(s)	132
AlwNI	CAGNNNCTG	1 cut(s)	851
Ama87I	CYCGRG	2 cut(s)	431, 566
AoxI	GGCC	4 cut(s)	210, 618, 651, 1375
ApaLI	GTGCAC	1 cut(s)	352
ApeKI	GCWGC	5 cut(s)	93, 275, 969, 1070, 1196
ArsI	GACNNNNNNTTYG	4 cut(s)	1043, 1075, 1580, 1612
Asp718I	GGTACC	1 cut(s)	981
AspA2I	CCTAGG	1 cut(s)	1234
AspS9I	GGNCC	3 cut(s)	210, 936, 1376
AsuC2I	CCSGG	4 cut(s)	209, 567, 568, 1380
AsuHPI	GGTGA	1 cut(s)	1370
AsuNHI	GCTAGC	1 cut(s)	196
AvaI	CYCGRG	2 cut(s)	431, 566
AvaII	GGWCC	1 cut(s)	936
AvrII	CCTAGG	1 cut(s)	1234
BaeGI	GKGCMC	1 cut(s)	356
BalI	TGGCCA	1 cut(s)	620
BanI	GGYRCC	2 cut(s)	686, 981
BanII	GRGCYC	3 cut(s)	248, 300, 432
BauI	CACGAG	2 cut(s)	611, 876
BbrPI	CACGTG	1 cut(s)	266
BbsI	GAAGAC	3 cut(s)	791, 1246, 1283
Bbv12I	GWGCWC	3 cut(s)	300, 356, 432
BbvCI	CCTCAGC	1 cut(s)	525
BbvI	GCAGC	5 cut(s)	105, 262, 981, 1057, 1183
BccI	CCATC	3 cut(s)	760, 767, 857
BceAI	ACGGC	2 cut(s)	1492, 1530
BcgI	CGANNNNNNTGC	2 cut(s)	1575, 1609
BciT130I	CCWGG	1 cut(s)	359
BclI	TGATCA	1 cut(s)	483
BcnI	CCSGG	4 cut(s)	209, 567, 568, 1380
BcuI	ACTAGT	1 cut(s)	1430
BfaI	CTAG	7 cut(s)	197, 203, 293, 342, 1235, 1431, 1707
BfmI	CTRYAG	2 cut(s)	843, 1047
BfuAI	ACCTGC	2 cut(s)	1403, 1547
BglII	AGATCT	1 cut(s)	946
BisI	GCNGC	6 cut(s)	94, 276, 669, 970, 1071, 1197
BlnI	CCTAGG	1 cut(s)	1234
BlpI	GCTNAGC	1 cut(s)	380
BlsI	GCNGC	6 cut(s)	95, 277, 670, 971, 1072, 1198
Bme1390I	CCNGG	5 cut(s)	209, 359, 567, 568, 1380
Bme18I	GGWCC	1 cut(s)	936
BmeT110I	CYCGRG	2 cut(s)	431, 566
BmgT120I	GGNCC	3 cut(s)	210, 936, 1376
BmiI	GGNNCC	4 cut(s)	572, 688, 983, 1378
BmrFI	CCNGG	5 cut(s)	209, 359, 567, 568, 1380
BmrI	ACTGGG	1 cut(s)	166
BmsI	GCATC	3 cut(s)	14, 355, 1669
BmtI	GCTAGC	1 cut(s)	200
BmuI	ACTGGG	1 cut(s)	166
BoxI	GACNNNNGTC	1 cut(s)	288
BpiI	GAAGAC	3 cut(s)	791, 1246, 1283
BpmI	CTGGAG	2 cut(s)	386, 1539
Bpu10I	CCTNAGC	1 cut(s)	525
Bpu1102I	GCTNAGC	1 cut(s)	380
BpuEI	CTTGAG	1 cut(s)	1119
BpuMI	CCSGG	4 cut(s)	209, 567, 568, 1380
BsaAI	YACGTR	2 cut(s)	266, 511
BsaJI	CCNNGG	7 cut(s)	357, 565, 566, 654, 1234, 1244, 1378
BsaWI	WCCGGW	4 cut(s)	573, 857, 933, 938
Bsc4I	CCNNNNNNNGG	8 cut(s)	207, 208, 241, 432, 573, 627, 864, 925
Bse118I	RCCGGY	1 cut(s)	902
Bse1I	ACTGG	2 cut(s)	172, 1378
Bse3DI	GCAATG	2 cut(s)	1072, 1470
BseBI	CCWGG	1 cut(s)	359
BseDI	CCNNGG	7 cut(s)	357, 565, 566, 654, 1234, 1244, 1378
BseGI	GGATG	1 cut(s)	370
BseLI	CCNNNNNNNGG	8 cut(s)	207, 208, 241, 432, 573, 627, 864, 925
BseMI	GCAATG	2 cut(s)	1072, 1470
BseMII	CTCAG	4 cut(s)	122, 383, 394, 539
BseNI	ACTGG	2 cut(s)	172, 1378
BseRI	GAGGAG	1 cut(s)	831
BseSI	GKGCMC	1 cut(s)	356
BseXI	GCAGC	5 cut(s)	105, 262, 981, 1057, 1183
BseYI	CCCAGC	1 cut(s)	192
BsgI	GTGCAG	1 cut(s)	112
Bsh1236I	CGCG	1 cut(s)	671
BshFI	GGCC	4 cut(s)	212, 620, 653, 1377
BshNI	GGYRCC	2 cut(s)	686, 981
BsiHKAI	GWGCWC	3 cut(s)	300, 356, 432
BsiHKCI	CYCGRG	2 cut(s)	431, 566
BsiSI	CCGG	8 cut(s)	208, 567, 574, 858, 903, 934, 939, 1380
BslFI	GGGAC	1 cut(s)	296
BslI	CCNNNNNNNGG	8 cut(s)	207, 208, 241, 432, 573, 627, 864, 925
BsmFI	GGGAC	1 cut(s)	296
BsnI	GGCC	4 cut(s)	212, 620, 653, 1377
BsoBI	CYCGRG	2 cut(s)	431, 566
Bsp1286I	GDGCHC	4 cut(s)	248, 300, 356, 432
Bsp143I	GATC	5 cut(s)	76, 124, 483, 680, 946
Bsp1720I	GCTNAGC	1 cut(s)	380
Bsp19I	CCATGG	1 cut(s)	1244
BspACI	CCGC	5 cut(s)	387, 531, 669, 690, 1570
BspANI	GGCC	4 cut(s)	212, 620, 653, 1377
BspCNI	CTCAG	4 cut(s)	123, 382, 393, 538
BspFNI	CGCG	1 cut(s)	671
BspHI	TCATGA	1 cut(s)	1272
BspLI	GGNNCC	4 cut(s)	572, 688, 983, 1378
BspMI	ACCTGC	2 cut(s)	1403, 1547
BspOI	GCTAGC	1 cut(s)	200
BspPI	GGATC	1 cut(s)	132
BspT107I	GGYRCC	2 cut(s)	686, 981
BsrBI	CCGCTC	1 cut(s)	389
BsrDI	GCAATG	2 cut(s)	1072, 1470
BsrFI	RCCGGY	1 cut(s)	902
BsrI	ACTGG	2 cut(s)	172, 1378
BssAI	RCCGGY	1 cut(s)	902
BssECI	CCNNGG	7 cut(s)	357, 565, 566, 654, 1234, 1244, 1378
BssMI	GATC	5 cut(s)	76, 124, 483, 680, 946
BssSI	CACGAG	2 cut(s)	611, 876
BssT1I	CCWWGG	2 cut(s)	1234, 1244
Bst2BI	CACGAG	2 cut(s)	611, 876
Bst2UI	CCWGG	1 cut(s)	359
Bst4CI	ACNGT	7 cut(s)	516, 649, 847, 1048, 1184, 1267, 1459
BstAPI	GCANNNNNTGC	3 cut(s)	275, 351, 665
BstBAI	YACGTR	2 cut(s)	266, 511
BstC8I	GCNNGC	6 cut(s)	198, 219, 456, 531, 1494, 1647
BstDEI	CTNAG	5 cut(s)	131, 369, 380, 525, 1214
BstDSI	CCRYGG	1 cut(s)	1244
BstEII	GGTNACC	1 cut(s)	1415
BstF5I	GGATG	1 cut(s)	370
BstFNI	CGCG	1 cut(s)	671
BstKTI	GATC	5 cut(s)	79, 127, 486, 683, 949
BstMBI	GATC	5 cut(s)	76, 124, 483, 680, 946
BstMWI	GCNNNNNNNGC	5 cut(s)	218, 275, 351, 665, 848
BstNI	CCWGG	1 cut(s)	359
BstNSI	RCATGY	2 cut(s)	221, 411
BstPAI	GACNNNNGTC	1 cut(s)	288
BstPI	GGTNACC	1 cut(s)	1415
BstSCI	CCNGG	5 cut(s)	207, 357, 565, 566, 1378
BstSFI	CTRYAG	2 cut(s)	843, 1047
BstSLI	GKGCMC	1 cut(s)	356
BstSNI	TACGTA	1 cut(s)	511
BstUI	CGCG	1 cut(s)	671
BstV1I	GCAGC	5 cut(s)	105, 262, 981, 1057, 1183
BstV2I	GAAGAC	3 cut(s)	791, 1246, 1283
BstX2I	RGATCY	2 cut(s)	124, 946
BstYI	RGATCY	2 cut(s)	124, 946
BsuRI	GGCC	4 cut(s)	212, 620, 653, 1377
BtgI	CCRYGG	1 cut(s)	1244
BtgZI	GCGATG	1 cut(s)	473
BtsCI	GGATG	1 cut(s)	370
BtsI	GCAGTG	2 cut(s)	897, 930
BtsIMutI	CAGTG	4 cut(s)	897, 930, 960, 1455
BveI	ACCTGC	2 cut(s)	1403, 1547
Cac8I	GCNNGC	6 cut(s)	198, 219, 456, 531, 1494, 1647
CaiI	CAGNNNCTG	1 cut(s)	851
CciI	TCATGA	1 cut(s)	1272
Cfr10I	RCCGGY	1 cut(s)	902
Cfr13I	GGNCC	3 cut(s)	210, 936, 1376
Cfr9I	CCCGGG	1 cut(s)	566
CpoI	CGGWCCG	1 cut(s)	936
CseI	GACGC	1 cut(s)	660
Csp6I	GTAC	9 cut(s)	56, 512, 517, 982, 1103, 1231, 1329, 1351, 1745
CspI	CGGWCCG	1 cut(s)	936
CviQI	GTAC	9 cut(s)	56, 512, 517, 982, 1103, 1231, 1329, 1351, 1745
DdeI	CTNAG	5 cut(s)	131, 369, 380, 525, 1214
DpnI	GATC	5 cut(s)	78, 126, 485, 682, 948
DpnII	GATC	5 cut(s)	76, 124, 483, 680, 946
DraIII	CACNNNGTG	1 cut(s)	351
EaeI	YGGCCR	2 cut(s)	618, 651
EciI	GGCGGA	1 cut(s)	1585
Ecl136II	GAGCTC	2 cut(s)	298, 430
Eco105I	TACGTA	1 cut(s)	511
Eco130I	CCWWGG	2 cut(s)	1234, 1244
Eco24I	GRGCYC	3 cut(s)	248, 300, 432
Eco32I	GATATC	2 cut(s)	186, 1732
Eco47I	GGWCC	1 cut(s)	936
Eco53kI	GAGCTC	2 cut(s)	298, 430
Eco57I	CTGAAG	2 cut(s)	1377, 1680
Eco72I	CACGTG	1 cut(s)	266
Eco88I	CYCGRG	2 cut(s)	431, 566
Eco91I	GGTNACC	1 cut(s)	1415
EcoICRI	GAGCTC	2 cut(s)	298, 430
EcoO65I	GGTNACC	1 cut(s)	1415
EcoRII	CCWGG	1 cut(s)	357
EcoRV	GATATC	2 cut(s)	186, 1732
EcoT14I	CCWWGG	2 cut(s)	1234, 1244
EcoT22I	ATGCAT	1 cut(s)	219
EcoT38I	GRGCYC	3 cut(s)	248, 300, 432
ErhI	CCWWGG	2 cut(s)	1234, 1244
FaqI	GGGAC	1 cut(s)	296
FauI	CCCGC	1 cut(s)	538
FbaI	TGATCA	1 cut(s)	483
FblI	GTMKAC	2 cut(s)	1002, 1050
Fnu4HI	GCNGC	6 cut(s)	94, 276, 669, 970, 1071, 1197
FokI	GGATG	1 cut(s)	377
FriOI	GRGCYC	3 cut(s)	248, 300, 432
Fsp4HI	GCNGC	6 cut(s)	94, 276, 669, 970, 1071, 1197
FspBI	CTAG	7 cut(s)	197, 203, 293, 342, 1235, 1431, 1707
GluI	GCNGC	6 cut(s)	94, 276, 669, 970, 1071, 1197
GsaI	CCCAGC	1 cut(s)	196
GsuI	CTGGAG	2 cut(s)	386, 1539
HaeIII	GGCC	4 cut(s)	212, 620, 653, 1377
HapII	CCGG	8 cut(s)	208, 567, 574, 858, 903, 934, 939, 1380
HgaI	GACGC	1 cut(s)	660
HincII	GTYRAC	3 cut(s)	562, 591, 750
HindII	GTYRAC	3 cut(s)	562, 591, 750
HinfI	GANTC	4 cut(s)	607, 1345, 1606, 1703
HpaI	GTTAAC	1 cut(s)	562
HpaII	CCGG	8 cut(s)	208, 567, 574, 858, 903, 934, 939, 1380
HphI	GGTGA	1 cut(s)	1370
Hpy166II	GTNNAC	8 cut(s)	354, 562, 591, 750, 1003, 1051, 1455, 1512
Hpy188III	TCNNGA	7 cut(s)	181, 293, 403, 878, 1260, 1273, 1707
Hpy8I	GTNNAC	8 cut(s)	354, 562, 591, 750, 1003, 1051, 1455, 1512
Hpy99I	CGWCG	1 cut(s)	676
HpyAV	CCTTC	3 cut(s)	410, 595, 706
HpyCH4III	ACNGT	7 cut(s)	516, 649, 847, 1048, 1184, 1267, 1459
HpyCH4IV	ACGT	6 cut(s)	265, 471, 510, 735, 1229, 1740
HpyF10VI	GCNNNNNNNGC	5 cut(s)	218, 275, 351, 665, 848
HpyF3I	CTNAG	5 cut(s)	131, 369, 380, 525, 1214
HpySE526I	ACGT	6 cut(s)	265, 471, 510, 735, 1229, 1740
KpnI	GGTACC	1 cut(s)	985
Ksp22I	TGATCA	1 cut(s)	483
KspAI	GTTAAC	1 cut(s)	562
Kzo9I	GATC	5 cut(s)	76, 124, 483, 680, 946
LmnI	GCTCC	2 cut(s)	1143, 1520
Lsp1109I	GCAGC	5 cut(s)	105, 262, 981, 1057, 1183
LweI	GCATC	3 cut(s)	14, 355, 1669
MaeI	CTAG	7 cut(s)	197, 203, 293, 342, 1235, 1431, 1707
MaeII	ACGT	6 cut(s)	265, 471, 510, 735, 1229, 1740
MaeIII	GTNAC	4 cut(s)	62, 853, 878, 1415
MalI	GATC	5 cut(s)	78, 126, 485, 682, 948
MbiI	CCGCTC	1 cut(s)	389
MboI	GATC	5 cut(s)	76, 124, 483, 680, 946
MboII	GAAGA	6 cut(s)	119, 169, 796, 1251, 1288, 1428
MflI	RGATCY	2 cut(s)	124, 946
MhlI	GDGCHC	4 cut(s)	248, 300, 356, 432
MlsI	TGGCCA	1 cut(s)	620
MluCI	AATT	5 cut(s)	478, 637, 835, 1016, 1087
MluNI	TGGCCA	1 cut(s)	620
MlyI	GAGTC	2 cut(s)	1339, 1712
MmeI	TCCRAC	5 cut(s)	420, 575, 1032, 1121, 1296
Mox20I	TGGCCA	1 cut(s)	620
Mph1103I	ATGCAT	1 cut(s)	219
MscI	TGGCCA	1 cut(s)	620
MseI	TTAA	7 cut(s)	20, 159, 504, 561, 1299, 1404, 1461
MslI	CAYNNNNRTG	3 cut(s)	115, 1502, 1654
Msp20I	TGGCCA	1 cut(s)	620
MspI	CCGG	8 cut(s)	208, 567, 574, 858, 903, 934, 939, 1380
MspR9I	CCNGG	5 cut(s)	209, 359, 567, 568, 1380
MvaI	CCWGG	1 cut(s)	359
MvnI	CGCG	1 cut(s)	671
MwoI	GCNNNNNNNGC	5 cut(s)	218, 275, 351, 665, 848
NciI	CCSGG	4 cut(s)	209, 567, 568, 1380
NcoI	CCATGG	1 cut(s)	1244
NdeII	GATC	5 cut(s)	76, 124, 483, 680, 946
NheI	GCTAGC	1 cut(s)	196
NlaIV	GGNNCC	4 cut(s)	572, 688, 983, 1378
NmeAIII	GCCGAG	3 cut(s)	437, 679, 1283
NmuCI	GTSAC	1 cut(s)	878
NsiI	ATGCAT	1 cut(s)	219
NspI	RCATGY	2 cut(s)	221, 411
OliI	CACNNNNGTG	1 cut(s)	1502
PaeI	GCATGC	1 cut(s)	221
PagI	TCATGA	1 cut(s)	1272
PfeI	GAWTC	2 cut(s)	607, 1606
PflMI	CCANNNNNTGG	1 cut(s)	925
PkrI	GCNGC	6 cut(s)	95, 277, 670, 971, 1072, 1198
PleI	GAGTC	2 cut(s)	1339, 1711
PmaCI	CACGTG	1 cut(s)	266
PmlI	CACGTG	1 cut(s)	266
PpsI	GAGTC	2 cut(s)	1339, 1711
Ppu21I	YACGTR	2 cut(s)	266, 511
PshAI	GACNNNNGTC	1 cut(s)	288
Psp124BI	GAGCTC	2 cut(s)	300, 432
Psp1406I	AACGTT	3 cut(s)	471, 735, 1740
Psp6I	CCWGG	1 cut(s)	357
PspCI	CACGTG	1 cut(s)	266
PspEI	GGTNACC	1 cut(s)	1415
PspFI	CCCAGC	1 cut(s)	192
PspGI	CCWGG	1 cut(s)	357
PspN4I	GGNNCC	4 cut(s)	572, 688, 983, 1378
PspPI	GGNCC	3 cut(s)	210, 936, 1376
PstNI	CAGNNNCTG	1 cut(s)	851
PsuI	RGATCY	2 cut(s)	124, 946
RsaI	GTAC	9 cut(s)	57, 513, 518, 983, 1104, 1232, 1330, 1352, 1746
RsaNI	GTAC	9 cut(s)	56, 512, 517, 982, 1103, 1231, 1329, 1351, 1745
RseI	CAYNNNNRTG	3 cut(s)	115, 1502, 1654
Rsr2I	CGGWCCG	1 cut(s)	936
RsrII	CGGWCCG	1 cut(s)	936
SacI	GAGCTC	2 cut(s)	300, 432
SaqAI	TTAA	7 cut(s)	20, 159, 504, 561, 1299, 1404, 1461
SatI	GCNGC	6 cut(s)	94, 276, 669, 970, 1071, 1197
Sau3AI	GATC	5 cut(s)	76, 124, 483, 680, 946
Sau96I	GGNCC	3 cut(s)	210, 936, 1376
SchI	GAGTC	2 cut(s)	1339, 1712
ScrFI	CCNGG	5 cut(s)	209, 359, 567, 568, 1380
SduI	GDGCHC	4 cut(s)	248, 300, 356, 432
SfaNI	GCATC	3 cut(s)	14, 355, 1669
SfcI	CTRYAG	2 cut(s)	843, 1047
SinI	GGWCC	1 cut(s)	936
SmaI	CCCGGG	1 cut(s)	568
SmiMI	CAYNNNNRTG	3 cut(s)	115, 1502, 1654
SmlI	CTYRAG	1 cut(s)	1134
SmoI	CTYRAG	1 cut(s)	1134
SnaBI	TACGTA	1 cut(s)	511
SpeI	ACTAGT	1 cut(s)	1430
SphI	GCATGC	1 cut(s)	221
Sse9I	AATT	5 cut(s)	478, 637, 835, 1016, 1087
SsiI	CCGC	5 cut(s)	387, 531, 669, 690, 1570
SspI	AATATT	2 cut(s)	757, 1096
SspMI	CTAG	7 cut(s)	197, 203, 293, 342, 1235, 1431, 1707
SstI	GAGCTC	2 cut(s)	300, 432
StyD4I	CCNGG	5 cut(s)	207, 357, 565, 566, 1378
StyI	CCWWGG	2 cut(s)	1234, 1244
TaaI	ACNGT	7 cut(s)	516, 649, 847, 1048, 1184, 1267, 1459
TaiI	ACGT	6 cut(s)	268, 474, 513, 738, 1232, 1743
TaqI	TCGA	3 cut(s)	464, 674, 1261
TasI	AATT	5 cut(s)	478, 637, 835, 1016, 1087
TatI	WGTACW	1 cut(s)	1350
TauI	GCSGC	1 cut(s)	671
TfiI	GAWTC	2 cut(s)	607, 1606
Tru1I	TTAA	7 cut(s)	20, 159, 504, 561, 1299, 1404, 1461
Tru9I	TTAA	7 cut(s)	20, 159, 504, 561, 1299, 1404, 1461
TscAI	CASTG	4 cut(s)	904, 930, 960, 1462
TseFI	GTSAC	1 cut(s)	878
TseI	GCWGC	5 cut(s)	93, 275, 969, 1070, 1196
Tsp45I	GTSAC	1 cut(s)	878
TspDTI	ATGAA	9 cut(s)	22, 1045, 1166, 1261, 1289, 1374, 1382, 1459, 1619
TspMI	CCCGGG	1 cut(s)	566
TspRI	CASTG	4 cut(s)	904, 930, 960, 1462
Van91I	CCANNNNNTGG	1 cut(s)	925
VneI	GTGCAC	1 cut(s)	352
VpaK11BI	GGWCC	1 cut(s)	936
XbaI	TCTAGA	2 cut(s)	292, 1706
XceI	RCATGY	2 cut(s)	221, 411
XmaI	CCCGGG	1 cut(s)	566
XmaJI	CCTAGG	1 cut(s)	1234
XmiI	GTMKAC	2 cut(s)	1002, 1050
XspI	CTAG	7 cut(s)	197, 203, 293, 342, 1235, 1431, 1707
Zsp2I	ATGCAT	1 cut(s)	219

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.