Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0004889

Physical Location & Seq

Genomic Coordinates Reverse (-)

32220370 .. 32222675

Gene Structure 2306 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1255000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1560 bp

ATGGCAACAAACCTAGTTAGTTCATTCTTCCTTTGTGTCTCCTTTGCTCTTTTCTTCTCACCGGCCCTTTCAAACATTCTCAACACGGGCATAGATTCATGGTGCAAAAAAACCCCCTACCCTGAAACTTGCAAATATACATTGACCCATGACCAAAAATCTCTCTCCATCCCCAACAAAGTATCCGACTTCAAGAAACTTGCAATCCAAGTGGCACTAAAAAGAGCACTCAAAGCCCAAAGTCACAACAAGTGGCTGGGGCAAAAGTGTAGAAACAAGAAAGAAAAGGCTGCATGGGCCGACTGTTTGAGCCTTTATGAAGATACCATCAAACTCCTCAACCAAACTGTAGACCCTGCCATAAAATGCACCGACTACGACTCACAAACTTGGCTCAGCACTGCCCTCACAAATCTTGACACATGTCGGGCCGGGTTCGTGGAGCTTGGGGTTTCGGACTTTGTCTTGCCCCTTATGTCCAACAATGTGTCCAAGCTCATTAGTAACAGTCTGTCGCTCGGGAATAGCTCAAATGTTCCACCTGTGACAAATTTGTACACGGAGGGTTTTCCTAGTTGGGTGTCACCGGGTGATAGGAAACTGTTGCAGTCGACACCTGCGGCGGATGTAGTGGTTGCACAAGATGGGTCGGGGAACTATAAGACGATCAAAGAGGCGTTGGATGTTGCAGCGAAGAGGAGTGGGAGTAAGAGGTTTGTGATACATGTGAAGAGAGGGGTTTATAAGGAGAACCTTGATATCAAGTTGAAGAATATTTTTCTACTTGGTGATGGGTTGAGGTATACAATTATCACAGGCAGCCGTAGTGTTGTAGGTGGTTCTACAACTTTCAATTCTGCAACCGTGGCGGTAACTGGTGAAGGCTTCATTGCTCGTGGCATAACATTCCGCAACACAGCTGGTCCAAAGAACCATCAAGCAGTAGCCCTCCGATCAGGCGCCGACCTCTCTGTTTTCTACCTTTGCAGCTTCGAAGGCTACCAAGACACCCTCTACACCCACTCCCAACGACAGTTTTACAAAGAATGCTACGTCTATGGCACCGTTGACTTCATCTTTGGCAACGCAGCCGTGGTTCTCCAAAACTGCATGATTTACGCAAGAAAGCCCATGAGTGGTCAAAAGAACACAATCACAGCCCAGGGACGAACCGACCCAAACCAGAACACGGGCATTTCTATCCACGACTCGCGTGTCATGGCCGCACCGGACTTCAAATCGTCTGTCGGCTCAGTCAAGACATTCTTGGGTAGGCCATGGAAGGAGTATTCACGCACAGTTTTTATGCAATCTTTCCTAGACACCCTGGTTGACCCAGCTGGGTGGCTCGAGTGGAGCGGTAATTTTGCTTTGAAGACATTGTATTATGGAGAATACAAGAACATTGGACCAGGTGCACCTACTAGTGGCAGGGTCAAGTGGGGTGGTTATCGAGTTATAACTAGTTCTTCAAAAGCATCGAAGTTTACTGTGAATAATTTCATTGCTGGAAGCTCATGGTTGCCCGCTACAAATGTGCCATTCACTGCTGGCCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0016020 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0042742 GO:0043086 GO:0043207 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0050829 GO:0050896 GO:0051704 GO:0051707 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098542 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

520

Amino Acids

56.99

Weight (kDa)

9.44

Isoelectric Point (pI)

33.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	31 - 173	2e-32	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	209 - 504	1.7e-142	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004889)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G45220
fragaria_vesca	FvH4_7g11320 FvH4_7g27760
malus_domestica	MD02G1207900.v1.1 MD07G1255000.v1.1 MD11G1195500.v1.1
prunus_persica	Prupe.2G141000_v2.0.a1 Prupe.2G278200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0349521 RchiOBHm_Chr1g0374551
rosa_laevigata	RLG00000026729 RLG00000028541 RLG00000028544
rosa_multiflora	Rmu_sc0002495.1_g000016 Rmu_sc0002495.1_g000029 Rmu_sc0004947.1_g000001
rosa_roxburghii	Rroxscaffold_4G00283410 Rroxscaffold_4G00305490
rosa_rugosa	Rorug01G0204300 Rorug01G0384100
rosa_samantha	Rh1AG394800 Rh1BG187700 Rh1BG359500 Rh1CG206500 Rh1CG372600 Rh1DG217200 Rh1DG390100
rosa_wichuraiana	Rw1G018840 Rw1G018870 Rw1G035010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	744, 1460
AarI	CACCTGC	1 cut(s)	625
AasI	GACNNNNNNGTC	1 cut(s)	1214
Acc36I	ACCTGC	1 cut(s)	625
AccB1I	GGYRCC	2 cut(s)	959, 1061
AccBSI	CCGCTC	1 cut(s)	1359
AccI	GTMKAC	3 cut(s)	351, 611, 803
AccII	CGCG	1 cut(s)	1213
AciI	CCGC	7 cut(s)	620, 623, 869, 910, 1224, 1359, 1527
AcoI	YGGCCR	1 cut(s)	1221
AcsI	RAATTY	1 cut(s)	550
AcyI	GRCGYC	1 cut(s)	960
AdeI	CACNNNGTG	1 cut(s)	590
AfaI	GTAC	1 cut(s)	557
AfiI	CCNNNNNNNGG	3 cut(s)	1136, 1189, 1427
AflIII	ACRYGT	2 cut(s)	422, 724
AgsI	TTSAA	7 cut(s)	72, 193, 769, 853, 1237, 1375, 1473
AhlI	ACTAGT	2 cut(s)	1424, 1463
AjnI	CCWGG	3 cut(s)	1161, 1326, 1411
AluBI	AGCT	7 cut(s)	445, 496, 528, 920, 990, 1340, 1515
AluI	AGCT	7 cut(s)	445, 496, 528, 920, 990, 1340, 1515
Alw21I	GWGCWC	2 cut(s)	229, 1420
Alw26I	GTCTC	1 cut(s)	43
Alw44I	GTGCAC	1 cut(s)	1416
Ama87I	CYCGRG	2 cut(s)	518, 1349
AoxI	GGCC	6 cut(s)	63, 297, 429, 1221, 1274, 1552
ApaLI	GTGCAC	1 cut(s)	1416
ApeKI	GCWGC	5 cut(s)	290, 689, 819, 987, 1088
ApoI	RAATTY	1 cut(s)	550
ArsI	GACNNNNNNTTYG	4 cut(s)	1061, 1093, 1230, 1262
Asp700I	GAANNNNTTC	1 cut(s)	1499
AspLEI	GCGC	1 cut(s)	962
AspS9I	GGNCC	5 cut(s)	64, 297, 429, 923, 1409
AsuC2I	CCSGG	2 cut(s)	433, 588
AsuHPI	GGTGA	5 cut(s)	51, 576, 602, 800, 890
AsuII	TTCGAA	1 cut(s)	993
AvaI	CYCGRG	2 cut(s)	518, 1349
AvaII	GGWCC	2 cut(s)	923, 1409
BaeGI	GKGCMC	1 cut(s)	1420
BanI	GGYRCC	2 cut(s)	959, 1061
BauI	CACGAG	1 cut(s)	894
BbsI	GAAGAC	1 cut(s)	1382
Bbv12I	GWGCWC	2 cut(s)	229, 1420
BbvI	GCAGC	5 cut(s)	277, 701, 831, 999, 1100
BccI	CCATC	5 cut(s)	176, 335, 638, 785, 942
BceAI	ACGGC	2 cut(s)	807, 1076
BciT130I	CCWGG	3 cut(s)	1163, 1328, 1413
BciVI	GTATCC	1 cut(s)	193
BcnI	CCSGG	2 cut(s)	433, 588
BcoDI	GTCTC	1 cut(s)	43
BcuI	ACTAGT	2 cut(s)	1424, 1463
BfaI	CTAG	5 cut(s)	14, 573, 1319, 1425, 1464
BfmI	CTRYAG	1 cut(s)	348
BfoI	RGCGCY	1 cut(s)	963
BfuAI	ACCTGC	1 cut(s)	625
BfuI	GTATCC	1 cut(s)	193
BisI	GCNGC	7 cut(s)	291, 621, 690, 820, 988, 1089, 1224
BlpI	GCTNAGC	1 cut(s)	395
BlsI	GCNGC	7 cut(s)	292, 622, 691, 821, 989, 1090, 1225
Bme1390I	CCNGG	5 cut(s)	433, 588, 1163, 1328, 1413
Bme18I	GGWCC	2 cut(s)	923, 1409
BmeT110I	CYCGRG	2 cut(s)	518, 1349
BmgT120I	GGNCC	5 cut(s)	64, 297, 429, 923, 1409
BmiI	GGNNCC	2 cut(s)	961, 1063
BmrFI	CCNGG	5 cut(s)	433, 588, 1163, 1328, 1413
BmsI	GCATC	1 cut(s)	1487
BoxI	GACNNNNGTC	1 cut(s)	423
BpiI	GAAGAC	1 cut(s)	1382
Bpu1102I	GCTNAGC	1 cut(s)	395
Bpu14I	TTCGAA	1 cut(s)	993
BpuMI	CCSGG	2 cut(s)	433, 588
BsaHI	GRCGYC	1 cut(s)	960
BsaJI	CCNNGG	6 cut(s)	864, 1092, 1161, 1162, 1277, 1326
BsaWI	WCCGGW	1 cut(s)	1228
BsaXI	ACNNNNNCTCC	4 cut(s)	691, 721, 1007, 1037
Bsc4I	CCNNNNNNNGG	3 cut(s)	1136, 1189, 1427
Bse118I	RCCGGY	1 cut(s)	61
Bse1I	ACTGG	1 cut(s)	880
Bse3DI	GCAATG	2 cut(s)	888, 1503
BseBI	CCWGG	3 cut(s)	1163, 1328, 1413
BseDI	CCNNGG	6 cut(s)	864, 1092, 1161, 1162, 1277, 1326
BseGI	GGATG	3 cut(s)	168, 631, 688
BseLI	CCNNNNNNNGG	3 cut(s)	1136, 1189, 1427
BseMI	GCAATG	2 cut(s)	888, 1503
BseMII	CTCAG	2 cut(s)	409, 1266
BseNI	ACTGG	1 cut(s)	880
BseRI	GAGGAG	2 cut(s)	326, 712
BseSI	GKGCMC	1 cut(s)	1420
BseXI	GCAGC	5 cut(s)	277, 701, 831, 999, 1100
BseYI	CCCAGC	3 cut(s)	256, 1336, 1340
Bsh1236I	CGCG	1 cut(s)	1213
BshFI	GGCC	6 cut(s)	65, 299, 431, 1223, 1276, 1554
BshNI	GGYRCC	2 cut(s)	959, 1061
BsiHKAI	GWGCWC	2 cut(s)	229, 1420
BsiHKCI	CYCGRG	2 cut(s)	518, 1349
BsiSI	CCGG	4 cut(s)	62, 432, 587, 1229
BslFI	GGGAC	1 cut(s)	1179
BslI	CCNNNNNNNGG	3 cut(s)	1136, 1189, 1427
BsmAI	GTCTC	1 cut(s)	43
BsmFI	GGGAC	1 cut(s)	1179
BsmI	GAATGC	1 cut(s)	1052
BsnI	GGCC	6 cut(s)	65, 299, 431, 1223, 1276, 1554
BsoBI	CYCGRG	2 cut(s)	518, 1349
Bsp119I	TTCGAA	1 cut(s)	993
Bsp1286I	GDGCHC	2 cut(s)	229, 1420
Bsp1407I	TGTACA	1 cut(s)	555
Bsp143I	GATC	2 cut(s)	666, 953
Bsp1720I	GCTNAGC	1 cut(s)	395
Bsp19I	CCATGG	1 cut(s)	1277
BspACI	CCGC	7 cut(s)	620, 623, 869, 910, 1224, 1359, 1527
BspANI	GGCC	6 cut(s)	65, 299, 431, 1223, 1276, 1554
BspCNI	CTCAG	2 cut(s)	408, 1265
BspFNI	CGCG	1 cut(s)	1213
BspLI	GGNNCC	2 cut(s)	961, 1063
BspMI	ACCTGC	1 cut(s)	625
BspT104I	TTCGAA	1 cut(s)	993
BspT107I	GGYRCC	2 cut(s)	959, 1061
BsrBI	CCGCTC	1 cut(s)	1359
BsrDI	GCAATG	2 cut(s)	888, 1503
BsrFI	RCCGGY	1 cut(s)	61
BsrGI	TGTACA	1 cut(s)	555
BsrI	ACTGG	1 cut(s)	880
BssAI	RCCGGY	1 cut(s)	61
BssECI	CCNNGG	6 cut(s)	864, 1092, 1161, 1162, 1277, 1326
BssMI	GATC	2 cut(s)	666, 953
BssNAI	GTATAC	1 cut(s)	804
BssNI	GRCGYC	1 cut(s)	960
BssSI	CACGAG	1 cut(s)	894
BssT1I	CCWWGG	1 cut(s)	1277
Bst1107I	GTATAC	1 cut(s)	804
Bst2BI	CACGAG	1 cut(s)	894
Bst2UI	CCWGG	3 cut(s)	1163, 1328, 1413
Bst4CI	ACNGT	9 cut(s)	305, 349, 509, 603, 865, 1035, 1066, 1300, 1492
Bst6I	CTCTTC	2 cut(s)	689, 725
BstACI	GRCGYC	1 cut(s)	960
BstAUI	TGTACA	1 cut(s)	555
BstBI	TTCGAA	1 cut(s)	993
BstC8I	GCNNGC	2 cut(s)	1527, 1552
BstDEI	CTNAG	2 cut(s)	395, 1252
BstDSI	CCRYGG	3 cut(s)	864, 1092, 1277
BstF5I	GGATG	3 cut(s)	168, 631, 688
BstFNI	CGCG	1 cut(s)	1213
BstH2I	RGCGCY	1 cut(s)	963
BstHHI	GCGC	1 cut(s)	962
BstKTI	GATC	2 cut(s)	669, 956
BstMAI	GTCTC	1 cut(s)	43
BstMBI	GATC	2 cut(s)	666, 953
BstMWI	GCNNNNNNNGC	4 cut(s)	233, 296, 866, 996
BstNI	CCWGG	3 cut(s)	1163, 1328, 1413
BstNSI	RCATGY	2 cut(s)	426, 728
BstPAI	GACNNNNGTC	1 cut(s)	423
BstSCI	CCNGG	5 cut(s)	431, 586, 1161, 1326, 1411
BstSFI	CTRYAG	1 cut(s)	348
BstSLI	GKGCMC	1 cut(s)	1420
BstUI	CGCG	1 cut(s)	1213
BstV1I	GCAGC	5 cut(s)	277, 701, 831, 999, 1100
BstV2I	GAAGAC	1 cut(s)	1382
BstXI	CCANNNNNNTGG	1 cut(s)	1344
BstZ17I	GTATAC	1 cut(s)	804
BsuI	GTATCC	1 cut(s)	193
BsuRI	GGCC	6 cut(s)	65, 299, 431, 1223, 1276, 1554
BtgI	CCRYGG	3 cut(s)	864, 1092, 1277
BtsCI	GGATG	3 cut(s)	168, 631, 688
BtsI	GCAGTG	2 cut(s)	399, 1545
BtsIMutI	CAGTG	2 cut(s)	399, 1545
BveI	ACCTGC	1 cut(s)	625
Cac8I	GCNNGC	2 cut(s)	1527, 1552
CfoI	GCGC	1 cut(s)	962
Cfr10I	RCCGGY	1 cut(s)	61
Cfr13I	GGNCC	5 cut(s)	64, 297, 429, 923, 1409
CsiI	ACCWGGT	1 cut(s)	1411
Csp6I	GTAC	1 cut(s)	556
CspCI	CAANNNNNGTGG	4 cut(s)	192, 227, 1426, 1461
CviQI	GTAC	1 cut(s)	556
DdeI	CTNAG	2 cut(s)	395, 1252
DinI	GGCGCC	1 cut(s)	961
DpnI	GATC	2 cut(s)	668, 955
DpnII	GATC	2 cut(s)	666, 953
DraIII	CACNNNGTG	1 cut(s)	590
DrdI	GACNNNNNNGTC	1 cut(s)	1214
DseDI	GACNNNNNNGTC	1 cut(s)	1214
EaeI	YGGCCR	1 cut(s)	1221
Eam1104I	CTCTTC	2 cut(s)	689, 725
EarI	CTCTTC	2 cut(s)	689, 725
EciI	GGCGGA	1 cut(s)	638
Eco130I	CCWWGG	1 cut(s)	1277
Eco32I	GATATC	1 cut(s)	760
Eco47I	GGWCC	2 cut(s)	923, 1409
Eco88I	CYCGRG	2 cut(s)	518, 1349
EcoRII	CCWGG	3 cut(s)	1161, 1326, 1411
EcoRV	GATATC	1 cut(s)	760
EcoT14I	CCWWGG	1 cut(s)	1277
EgeI	GGCGCC	1 cut(s)	961
EheI	GGCGCC	1 cut(s)	961
ErhI	CCWWGG	1 cut(s)	1277
FalI	AAGNNNNNCTT	2 cut(s)	1250, 1282
FaqI	GGGAC	1 cut(s)	1179
FauI	CCCGC	1 cut(s)	1534
FblI	GTMKAC	3 cut(s)	351, 611, 803
Fnu4HI	GCNGC	7 cut(s)	291, 621, 690, 820, 988, 1089, 1224
FokI	GGATG	3 cut(s)	155, 638, 695
Fsp4HI	GCNGC	7 cut(s)	291, 621, 690, 820, 988, 1089, 1224
FspBI	CTAG	5 cut(s)	14, 573, 1319, 1425, 1464
GlaI	GCGC	1 cut(s)	961
GluI	GCNGC	7 cut(s)	291, 621, 690, 820, 988, 1089, 1224
GsaI	CCCAGC	3 cut(s)	260, 1340, 1344
HaeII	RGCGCY	1 cut(s)	963
HaeIII	GGCC	6 cut(s)	65, 299, 431, 1223, 1276, 1554
HapII	CCGG	4 cut(s)	62, 432, 587, 1229
HhaI	GCGC	1 cut(s)	962
Hin1I	GRCGYC	1 cut(s)	960
Hin6I	GCGC	1 cut(s)	960
HinP1I	GCGC	1 cut(s)	960
HincII	GTYRAC	3 cut(s)	612, 1069, 1333
HindII	GTYRAC	3 cut(s)	612, 1069, 1333
HinfI	GANTC	3 cut(s)	95, 380, 1208
HpaII	CCGG	4 cut(s)	62, 432, 587, 1229
HphI	GGTGA	5 cut(s)	51, 576, 602, 800, 890
Hpy166II	GTNNAC	8 cut(s)	352, 558, 612, 804, 1069, 1333, 1418, 1488
Hpy188I	TCNGA	3 cut(s)	187, 457, 953
Hpy188III	TCNNGA	4 cut(s)	193, 416, 520, 1258
Hpy8I	GTNNAC	8 cut(s)	352, 558, 612, 804, 1069, 1333, 1418, 1488
HpyAV	CCTTC	3 cut(s)	875, 989, 1276
HpyCH4III	ACNGT	9 cut(s)	305, 349, 509, 603, 865, 1035, 1066, 1300, 1492
HpyCH4IV	ACGT	1 cut(s)	1053
HpyF10VI	GCNNNNNNNGC	4 cut(s)	233, 296, 866, 996
HpyF3I	CTNAG	2 cut(s)	395, 1252
HpySE526I	ACGT	1 cut(s)	1053
Hsp92I	GRCGYC	1 cut(s)	960
HspAI	GCGC	1 cut(s)	960
KasI	GGCGCC	1 cut(s)	959
Kzo9I	GATC	2 cut(s)	666, 953
LmnI	GCTCC	2 cut(s)	442, 1356
Lsp1109I	GCAGC	5 cut(s)	277, 701, 831, 999, 1100
LweI	GCATC	1 cut(s)	1487
MabI	ACCWGGT	1 cut(s)	1411
MaeI	CTAG	5 cut(s)	14, 573, 1319, 1425, 1464
MaeII	ACGT	1 cut(s)	1053
MaeIII	GTNAC	5 cut(s)	242, 503, 544, 582, 871
MalI	GATC	2 cut(s)	668, 955
MbiI	CCGCTC	1 cut(s)	1359
MboI	GATC	2 cut(s)	666, 953
MboII	GAAGA	8 cut(s)	19, 46, 332, 706, 742, 781, 1387, 1461
MhlI	GDGCHC	2 cut(s)	229, 1420
MluCI	AATT	5 cut(s)	550, 807, 853, 1363, 1498
Mly113I	GGCGCC	1 cut(s)	960
MlyI	GAGTC	2 cut(s)	374, 1202
MmeI	TCCRAC	3 cut(s)	210, 504, 660
MroXI	GAANNNNTTC	1 cut(s)	1499
MspA1I	CMGCKG	2 cut(s)	920, 1340
MspI	CCGG	4 cut(s)	62, 432, 587, 1229
MspR9I	CCNGG	5 cut(s)	433, 588, 1163, 1328, 1413
Mva1269I	GAATGC	1 cut(s)	1052
MvaI	CCWGG	3 cut(s)	1163, 1328, 1413
MvnI	CGCG	1 cut(s)	1213
MwoI	GCNNNNNNNGC	4 cut(s)	233, 296, 866, 996
NarI	GGCGCC	1 cut(s)	960
NciI	CCSGG	2 cut(s)	433, 588
NcoI	CCATGG	1 cut(s)	1277
NdeII	GATC	2 cut(s)	666, 953
NlaIV	GGNNCC	2 cut(s)	961, 1063
NmuCI	GTSAC	3 cut(s)	242, 544, 582
NspI	RCATGY	2 cut(s)	426, 728
NspV	TTCGAA	1 cut(s)	993
PaeR7I	CTCGAG	1 cut(s)	1349
PaqCI	CACCTGC	1 cut(s)	625
PasI	CCCWGGG	1 cut(s)	1162
PciI	ACATGT	2 cut(s)	422, 724
PctI	GAATGC	1 cut(s)	1052
PdmI	GAANNNNTTC	1 cut(s)	1499
PfeI	GAWTC	1 cut(s)	95
PflFI	GACNNNGTC	1 cut(s)	461
PkrI	GCNGC	7 cut(s)	292, 622, 691, 821, 989, 1090, 1225
PleI	GAGTC	2 cut(s)	374, 1202
PluTI	GGCGCC	1 cut(s)	963
PpsI	GAGTC	2 cut(s)	374, 1202
PscI	ACATGT	2 cut(s)	422, 724
PshAI	GACNNNNGTC	1 cut(s)	423
PsiI	TTATAA	2 cut(s)	744, 1460
Psp6I	CCWGG	3 cut(s)	1161, 1326, 1411
PspFI	CCCAGC	3 cut(s)	256, 1336, 1340
PspGI	CCWGG	3 cut(s)	1161, 1326, 1411
PspN4I	GGNNCC	2 cut(s)	961, 1063
PspPI	GGNCC	5 cut(s)	64, 297, 429, 923, 1409
PspXI	VCTCGAGB	1 cut(s)	1349
PsyI	GACNNNGTC	1 cut(s)	461
PvuII	CAGCTG	2 cut(s)	920, 1340
RsaI	GTAC	1 cut(s)	557
RsaNI	GTAC	1 cut(s)	556
SalI	GTCGAC	1 cut(s)	610
SatI	GCNGC	7 cut(s)	291, 621, 690, 820, 988, 1089, 1224
Sau3AI	GATC	2 cut(s)	666, 953
Sau96I	GGNCC	5 cut(s)	64, 297, 429, 923, 1409
SchI	GAGTC	2 cut(s)	374, 1202
ScrFI	CCNGG	5 cut(s)	433, 588, 1163, 1328, 1413
SduI	GDGCHC	2 cut(s)	229, 1420
SexAI	ACCWGGT	1 cut(s)	1411
SfaNI	GCATC	1 cut(s)	1487
SfcI	CTRYAG	1 cut(s)	348
SfoI	GGCGCC	1 cut(s)	961
Sfr274I	CTCGAG	1 cut(s)	1349
SfuI	TTCGAA	1 cut(s)	993
SinI	GGWCC	2 cut(s)	923, 1409
SlaI	CTCGAG	1 cut(s)	1349
SmlI	CTYRAG	1 cut(s)	1349
SmoI	CTYRAG	1 cut(s)	1349
SpeI	ACTAGT	2 cut(s)	1424, 1463
Sse9I	AATT	5 cut(s)	550, 807, 853, 1363, 1498
SsiI	CCGC	7 cut(s)	620, 623, 869, 910, 1224, 1359, 1527
SspDI	GGCGCC	1 cut(s)	959
SspI	AATATT	1 cut(s)	775
SspMI	CTAG	5 cut(s)	14, 573, 1319, 1425, 1464
StyD4I	CCNGG	5 cut(s)	431, 586, 1161, 1326, 1411
StyI	CCWWGG	1 cut(s)	1277
TaaI	ACNGT	9 cut(s)	305, 349, 509, 603, 865, 1035, 1066, 1300, 1492
TaiI	ACGT	1 cut(s)	1056
TaqI	TCGA	5 cut(s)	611, 993, 1350, 1453, 1481
TasI	AATT	5 cut(s)	550, 807, 853, 1363, 1498
TatI	WGTACW	1 cut(s)	555
TauI	GCSGC	2 cut(s)	623, 1226
TfiI	GAWTC	1 cut(s)	95
TscAI	CASTG	2 cut(s)	406, 1552
TseFI	GTSAC	3 cut(s)	242, 544, 582
TseI	GCWGC	5 cut(s)	290, 689, 819, 987, 1088
Tsp45I	GTSAC	3 cut(s)	242, 544, 582
TspDTI	ATGAA	6 cut(s)	12, 87, 333, 877, 1063, 1492
TspGWI	ACGGA	1 cut(s)	575
TspRI	CASTG	2 cut(s)	406, 1552
Tth111I	GACNNNGTC	1 cut(s)	461
VneI	GTGCAC	1 cut(s)	1416
VpaK11BI	GGWCC	2 cut(s)	923, 1409
XapI	RAATTY	1 cut(s)	550
XceI	RCATGY	2 cut(s)	426, 728
XhoI	CTCGAG	1 cut(s)	1349
XmiI	GTMKAC	3 cut(s)	351, 611, 803
XmnI	GAANNNNTTC	1 cut(s)	1499
XspI	CTAG	5 cut(s)	14, 573, 1319, 1425, 1464

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.