AT2G47020

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0004116
Physical Location & Seq
Reverse (-)
19319685 .. 19322507
2823 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G47020.2

Sequence Viewer

Length: 1242 bp
ATGAGGGTGTTAATTCGGCCAAATTTCTTGAGCAATCTGATTCGATATTGCTCTAGAGGTACGCATTCACACGATCGTAGCCTGCGATCGGTTCTCTCGTCAAATATGATTCGTCTGTACACCACCGGAATGGAACCTCAGTTGTCTCCTGATCTTATTAAGATAATGGATCAGAGGCTCTCGGCGATAGAGCATCGAAATGCTGTCCTTCAGAAGCTTATCAACCAGCCTGAGTACTCACCAGAGGAGTTTTCAAGAGCCAATAAGGAACTTCGGAAGCTGAGAGATTCAATGTTGCTGATCAATGATTTGAGGGCCAAACAAAAGGAGATTGATGGATTGAAATCTCTGGTGTCGGAAAGTTCGGATGATAAAGACATGCTTGATTTGGCTGTAGGTGAATTAGACGAGGCGGTGGAAGAAGAAAAAAGACTTCAAACTTTGCTGCTTAAGTCTTTGCTTCCTAAAGATGAAGCTGATGAGAGGGATTGCATTTTGGAGGTGAGAGCAGGTACTGGTGGTGAAGAGGCTTCTTTGTTTGCCATGGACATATTCAGAATGTACGAAAGGTATTCACAGAAGAAAGGTTGGAAGTTTGATATTGTAGACATCACCGAGTCAGATATGAAAGGGTATAAAGAAGCCAGTGCTGCAATTTGTGGAGCCAGTGTCTACGGAAAACTAAAGTTCGAGAGTGGAATTCACAGGGTTCAGCGTATTCCTATCACAGAGAAATCTGGTCGAATTCACACTAGCGCTATATCTGTTGCCATCCTTCCCCAGGCTGATGAGGTAGATGTTCAGCTAAGGAATGAAGATTTGAGGATTGACACTTATAGGTCTGGTGGCTCAGGCGGTCAACACGCCAACACAACAAACAGCGCAGTCCGAATAATCCATCTTCCAACGGGGATGATGGTCTCAATCCAAGATGAAAGGTCACAACATATGAACAGAGCCAAAGCACTTAAAGTACTATGTGCAAGGCTATATGAAATCGAAAGGTTGAGAATACAGAGCAGTCGATCAAAGCTACGGTCAGATCAGATTGGCAGCGGAGACCGATCTGGACGAATCCGTACATACAATTTTCCACAAGGGCGAGTAACGGATCACCGTGTGGGAATCACTCACCATGCCATTGAGGATATGATGCAGGGAGAGAATCTGGACATGTTCATTGATGCCCTTCTCTTGCGCCAAGAAATGGACGCCATTGCTTCTTTCAGCTCCACTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PCRF RF-1

Protein Analysis

413

Amino Acids

46.83

Weight (kDa)

6.85

Isoelectric Point (pI)

42.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCRF PF03462 64 - 256 1.3e-60 PCRF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 500
AccB7I CCANNNNNTGG 1 cut(s) 1207
AccI GTMKAC 2 cut(s) 606, 672
AciI CCGC 3 cut(s) 413, 855, 1056
AclWI GGATC 2 cut(s) 177, 1119
AcoI YGGCCR 1 cut(s) 17
AcsI RAATTY 3 cut(s) 22, 699, 744
AcuI CTGAAG 1 cut(s) 194
AcyI GRCGYC 1 cut(s) 1212
AdeI CACNNNGTG 1 cut(s) 1120
AfaI GTAC 7 cut(s) 61, 119, 236, 514, 563, 975, 1081
AfeI AGCGCT 1 cut(s) 757
AfiI CCNNNNNNNGG 3 cut(s) 88, 781, 1207
AflII CTTAAG 1 cut(s) 449
AflIII ACRYGT 1 cut(s) 1173
AgsI TTSAA 4 cut(s) 255, 291, 343, 437
AjnI CCWGG 1 cut(s) 780
AluBI AGCT 6 cut(s) 217, 280, 476, 805, 1033, 1230
AluI AGCT 6 cut(s) 217, 280, 476, 805, 1033, 1230
Alw26I GTCTC 3 cut(s) 150, 925, 1053
AlwI GGATC 2 cut(s) 177, 1119
AlwNI CAGNNNCTG 1 cut(s) 515
Aor51HI AGCGCT 1 cut(s) 757
AoxI GGCC 2 cut(s) 17, 315
ApeKI GCWGC 3 cut(s) 445, 650, 1053
ApoI RAATTY 3 cut(s) 22, 699, 744
ArsI GACNNNNNNTTYG 2 cut(s) 1022, 1054
AspLEI GCGC 3 cut(s) 758, 884, 1200
AspS9I GGNCC 1 cut(s) 315
AsuHPI GGTGA 7 cut(s) 231, 410, 514, 533, 604, 1106, 1124
BbvI GCAGC 3 cut(s) 432, 637, 1065
BccI CCATC 4 cut(s) 329, 779, 906, 910
BciT130I CCWGG 1 cut(s) 782
BclI TGATCA 1 cut(s) 300
BcoDI GTCTC 3 cut(s) 150, 925, 1053
BfaI CTAG 2 cut(s) 54, 753
BfmI CTRYAG 1 cut(s) 393
BfoI RGCGCY 1 cut(s) 759
BfrI CTTAAG 1 cut(s) 449
BfuAI ACCTGC 1 cut(s) 500
BisI GCNGC 3 cut(s) 446, 651, 1054
BlsI GCNGC 3 cut(s) 447, 652, 1055
BmcAI AGTACT 2 cut(s) 236, 975
Bme1390I CCNGG 1 cut(s) 782
BmgT120I GGNCC 1 cut(s) 315
BmiI GGNNCC 2 cut(s) 135, 664
BmrFI CCNGG 1 cut(s) 782
BmsI GCATC 3 cut(s) 202, 1143, 1174
Bpu10I CCTNAGC 2 cut(s) 806, 850
BpuEI CTTGAG 1 cut(s) 49
BsaBI GATNNNNATC 1 cut(s) 343
BsaHI GRCGYC 1 cut(s) 1212
BsaI GGTCTC 2 cut(s) 925, 1053
BsaJI CCNNGG 2 cut(s) 543, 780
BsaWI WCCGGW 1 cut(s) 125
BsaXI ACNNNNNCTCC 2 cut(s) 654, 684
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 781, 1207
Bse1I ACTGG 3 cut(s) 520, 645, 666
Bse3DI GCAATG 1 cut(s) 1215
Bse8I GATNNNNATC 1 cut(s) 343
BseBI CCWGG 1 cut(s) 782
BseDI CCNNGG 2 cut(s) 543, 780
BseGI GGATG 3 cut(s) 373, 771, 918
BseJI GATNNNNATC 1 cut(s) 343
BseLI CCNNNNNNNGG 3 cut(s) 88, 781, 1207
BseMI GCAATG 1 cut(s) 1215
BseMII CTCAG 4 cut(s) 152, 222, 272, 864
BseNI ACTGG 3 cut(s) 520, 645, 666
BseRI GAGGAG 1 cut(s) 260
BseXI GCAGC 3 cut(s) 432, 637, 1065
Bsh1285I CGRYCG 2 cut(s) 76, 89
BshFI GGCC 2 cut(s) 19, 317
BsiEI CGRYCG 2 cut(s) 76, 89
BsiSI CCGG 1 cut(s) 126
BslI CCNNNNNNNGG 3 cut(s) 88, 781, 1207
BsmAI GTCTC 3 cut(s) 150, 925, 1053
BsmI GAATGC 1 cut(s) 64
BsnI GGCC 2 cut(s) 19, 317
Bso31I GGTCTC 2 cut(s) 925, 1053
Bsp1407I TGTACA 1 cut(s) 117
Bsp143I GATC 9 cut(s) 73, 86, 151, 169, 300, 1025, 1042, 1064, 1111
Bsp19I CCATGG 1 cut(s) 543
BspACI CCGC 3 cut(s) 413, 855, 1056
BspANI GGCC 2 cut(s) 19, 317
BspCNI CTCAG 4 cut(s) 151, 223, 273, 863
BspHI TCATGA 1 cut(s) 1238
BspLI GGNNCC 2 cut(s) 135, 664
BspMI ACCTGC 1 cut(s) 500
BspPI GGATC 2 cut(s) 177, 1119
BspTI CTTAAG 1 cut(s) 449
BspTNI GGTCTC 2 cut(s) 925, 1053
BsrDI GCAATG 1 cut(s) 1215
BsrGI TGTACA 1 cut(s) 117
BsrI ACTGG 3 cut(s) 520, 645, 666
BssECI CCNNGG 2 cut(s) 543, 780
BssMI GATC 9 cut(s) 73, 86, 151, 169, 300, 1025, 1042, 1064, 1111
BssNI GRCGYC 1 cut(s) 1212
BssT1I CCWWGG 1 cut(s) 543
Bst2UI CCWGG 1 cut(s) 782
Bst4CI ACNGT 2 cut(s) 1038, 1118
Bst6I CTCTTC 1 cut(s) 519
BstACI GRCGYC 1 cut(s) 1212
BstAFI CTTAAG 1 cut(s) 449
BstAUI TGTACA 1 cut(s) 117
BstC8I GCNNGC 1 cut(s) 83
BstDEI CTNAG 5 cut(s) 138, 231, 281, 806, 850
BstDSI CCRYGG 1 cut(s) 543
BstENI CCTNNNNNAGG 1 cut(s) 779
BstF5I GGATG 3 cut(s) 373, 771, 918
BstH2I RGCGCY 1 cut(s) 759
BstHHI GCGC 3 cut(s) 758, 884, 1200
BstKTI GATC 9 cut(s) 76, 89, 154, 172, 303, 1028, 1045, 1067, 1114
BstMAI GTCTC 3 cut(s) 150, 925, 1053
BstMBI GATC 9 cut(s) 73, 86, 151, 169, 300, 1025, 1042, 1064, 1111
BstMCI CGRYCG 2 cut(s) 76, 89
BstMWI GCNNNNNNNGC 1 cut(s) 650
BstNI CCWGG 1 cut(s) 782
BstNSI RCATGY 2 cut(s) 382, 1177
BstSCI CCNGG 1 cut(s) 780
BstSFI CTRYAG 1 cut(s) 393
BstV1I GCAGC 3 cut(s) 432, 637, 1065
BstXI CCANNNNNNTGG 1 cut(s) 130
BsuRI GGCC 2 cut(s) 19, 317
BtgI CCRYGG 1 cut(s) 543
BtsCI GGATG 3 cut(s) 373, 771, 918
BtsIMutI CAGTG 2 cut(s) 652, 673
BveI ACCTGC 1 cut(s) 500
Cac8I GCNNGC 1 cut(s) 83
CaiI CAGNNNCTG 1 cut(s) 515
CciI TCATGA 1 cut(s) 1238
CfoI GCGC 3 cut(s) 758, 884, 1200
Cfr13I GGNCC 1 cut(s) 315
CseI GACGC 1 cut(s) 1220
Csp6I GTAC 7 cut(s) 60, 118, 235, 513, 562, 974, 1080
CviAII CATG 5 cut(s) 379, 544, 1136, 1174, 1239
CviQI GTAC 7 cut(s) 60, 118, 235, 513, 562, 974, 1080
DdeI CTNAG 5 cut(s) 138, 231, 281, 806, 850
DpnI GATC 9 cut(s) 75, 88, 153, 171, 302, 1027, 1044, 1066, 1113
DpnII GATC 9 cut(s) 73, 86, 151, 169, 300, 1025, 1042, 1064, 1111
DraIII CACNNNGTG 1 cut(s) 1120
EaeI YGGCCR 1 cut(s) 17
Eam1104I CTCTTC 1 cut(s) 519
EarI CTCTTC 1 cut(s) 519
Eco130I CCWWGG 1 cut(s) 543
Eco31I GGTCTC 2 cut(s) 925, 1053
Eco47III AGCGCT 1 cut(s) 757
Eco57I CTGAAG 1 cut(s) 194
EcoNI CCTNNNNNAGG 1 cut(s) 779
EcoRI GAATTC 2 cut(s) 699, 744
EcoRII CCWGG 1 cut(s) 780
EcoT14I CCWWGG 1 cut(s) 543
ErhI CCWWGG 1 cut(s) 543
FaeI CATG 5 cut(s) 382, 547, 1139, 1177, 1242
FalI AAGNNNNNCTT 2 cut(s) 366, 398
FatI CATG 5 cut(s) 378, 543, 1135, 1173, 1238
FauNDI CATATG 1 cut(s) 948
FbaI TGATCA 1 cut(s) 300
FblI GTMKAC 2 cut(s) 606, 672
Fnu4HI GCNGC 3 cut(s) 446, 651, 1054
FokI GGATG 3 cut(s) 380, 758, 925
Fsp4HI GCNGC 3 cut(s) 446, 651, 1054
FspBI CTAG 2 cut(s) 54, 753
GlaI GCGC 3 cut(s) 757, 883, 1199
GluI GCNGC 3 cut(s) 446, 651, 1054
HaeII RGCGCY 1 cut(s) 759
HaeIII GGCC 2 cut(s) 19, 317
HapII CCGG 1 cut(s) 126
HgaI GACGC 1 cut(s) 1220
HhaI GCGC 3 cut(s) 758, 884, 1200
Hin1I GRCGYC 1 cut(s) 1212
Hin1II CATG 5 cut(s) 382, 547, 1139, 1177, 1242
Hin6I GCGC 3 cut(s) 756, 882, 1198
HinP1I GCGC 3 cut(s) 756, 882, 1198
HincII GTYRAC 1 cut(s) 860
HindII GTYRAC 1 cut(s) 860
HindIII AAGCTT 1 cut(s) 215
HinfI GANTC 7 cut(s) 40, 109, 287, 617, 1074, 1125, 1165
HpaII CCGG 1 cut(s) 126
HphI GGTGA 7 cut(s) 231, 410, 514, 533, 604, 1106, 1124
Hpy166II GTNNAC 4 cut(s) 120, 607, 673, 860
Hpy188III TCNNGA 8 cut(s) 28, 54, 149, 255, 691, 1068, 1169, 1239
Hpy8I GTNNAC 4 cut(s) 120, 607, 673, 860
HpyAV CCTTC 3 cut(s) 218, 785, 1199
HpyCH4III ACNGT 2 cut(s) 1038, 1118
HpyCH4V TGCA 4 cut(s) 492, 653, 983, 1156
HpyF10VI GCNNNNNNNGC 1 cut(s) 650
HpyF3I CTNAG 5 cut(s) 138, 231, 281, 806, 850
Hsp92I GRCGYC 1 cut(s) 1212
Hsp92II CATG 5 cut(s) 382, 547, 1139, 1177, 1242
HspAI GCGC 3 cut(s) 756, 882, 1198
Ksp22I TGATCA 1 cut(s) 300
Kzo9I GATC 9 cut(s) 73, 86, 151, 169, 300, 1025, 1042, 1064, 1111
LmnI GCTCC 2 cut(s) 662, 1235
Lsp1109I GCAGC 3 cut(s) 432, 637, 1065
LweI GCATC 3 cut(s) 202, 1143, 1174
MaeI CTAG 2 cut(s) 54, 753
MaeIII GTNAC 2 cut(s) 939, 1105
MalI GATC 9 cut(s) 75, 88, 153, 171, 302, 1027, 1044, 1066, 1113
MboI GATC 9 cut(s) 73, 86, 151, 169, 300, 1025, 1042, 1064, 1111
MboII GAAGA 6 cut(s) 431, 434, 536, 592, 827, 893
MluCI AATT 7 cut(s) 12, 22, 401, 654, 699, 744, 1087
MlyI GAGTC 1 cut(s) 626
MmeI TCCRAC 3 cut(s) 336, 569, 929
MseI TTAA 4 cut(s) 11, 159, 450, 969
MslI CAYNNNNRTG 2 cut(s) 128, 198
MspA1I CMGCKG 1 cut(s) 1056
MspCI CTTAAG 1 cut(s) 449
MspI CCGG 1 cut(s) 126
MspR9I CCNGG 1 cut(s) 782
Mva1269I GAATGC 1 cut(s) 64
MvaI CCWGG 1 cut(s) 782
MwoI GCNNNNNNNGC 1 cut(s) 650
NcoI CCATGG 1 cut(s) 543
NdeI CATATG 1 cut(s) 948
NdeII GATC 9 cut(s) 73, 86, 151, 169, 300, 1025, 1042, 1064, 1111
NlaIII CATG 5 cut(s) 382, 547, 1139, 1177, 1242
NlaIV GGNNCC 2 cut(s) 135, 664
NmeAIII GCCGAG 1 cut(s) 161
NmuCI GTSAC 1 cut(s) 939
NspI RCATGY 2 cut(s) 382, 1177
PagI TCATGA 1 cut(s) 1238
PciI ACATGT 1 cut(s) 1173
PcsI WCGNNNNNNNCGW 2 cut(s) 82, 95
PctI GAATGC 1 cut(s) 64
PfeI GAWTC 6 cut(s) 40, 109, 287, 1074, 1125, 1165
PflMI CCANNNNNTGG 1 cut(s) 1207
PkrI GCNGC 3 cut(s) 447, 652, 1055
Ple19I CGATCG 2 cut(s) 76, 89
PleI GAGTC 1 cut(s) 625
PpsI GAGTC 1 cut(s) 625
PscI ACATGT 1 cut(s) 1173
Psp6I CCWGG 1 cut(s) 780
PspGI CCWGG 1 cut(s) 780
PspN4I GGNNCC 2 cut(s) 135, 664
PspPI GGNCC 1 cut(s) 315
PstNI CAGNNNCTG 1 cut(s) 515
PvuI CGATCG 2 cut(s) 76, 89
RsaI GTAC 7 cut(s) 61, 119, 236, 514, 563, 975, 1081
RsaNI GTAC 7 cut(s) 60, 118, 235, 513, 562, 974, 1080
RseI CAYNNNNRTG 2 cut(s) 128, 198
SaqAI TTAA 4 cut(s) 11, 159, 450, 969
SatI GCNGC 3 cut(s) 446, 651, 1054
Sau3AI GATC 9 cut(s) 73, 86, 151, 169, 300, 1025, 1042, 1064, 1111
Sau96I GGNCC 1 cut(s) 315
ScaI AGTACT 2 cut(s) 236, 975
SchI GAGTC 1 cut(s) 626
ScrFI CCNGG 1 cut(s) 782
SfaNI GCATC 3 cut(s) 202, 1143, 1174
SfcI CTRYAG 1 cut(s) 393
SmiMI CAYNNNNRTG 2 cut(s) 128, 198
SmlI CTYRAG 2 cut(s) 28, 449
SmoI CTYRAG 2 cut(s) 28, 449
Sse9I AATT 7 cut(s) 12, 22, 401, 654, 699, 744, 1087
SsiI CCGC 3 cut(s) 413, 855, 1056
SspMI CTAG 2 cut(s) 54, 753
StyD4I CCNGG 1 cut(s) 780
StyI CCWWGG 1 cut(s) 543
TaaI ACNGT 2 cut(s) 1038, 1118
TaqI TCGA 6 cut(s) 43, 196, 690, 742, 999, 1024
TaqII GACCGA 1 cut(s) 1077
TasI AATT 7 cut(s) 12, 22, 401, 654, 699, 744, 1087
TatI WGTACW 3 cut(s) 117, 234, 973
TfiI GAWTC 6 cut(s) 40, 109, 287, 1074, 1125, 1165
Tru1I TTAA 4 cut(s) 11, 159, 450, 969
Tru9I TTAA 4 cut(s) 11, 159, 450, 969
TscAI CASTG 2 cut(s) 652, 673
TseFI GTSAC 1 cut(s) 939
TseI GCWGC 3 cut(s) 445, 650, 1053
Tsp45I GTSAC 1 cut(s) 939
TspDTI ATGAA 8 cut(s) 486, 641, 828, 948, 965, 1008, 1168, 1227
TspGWI ACGGA 3 cut(s) 690, 1067, 1124
TspRI CASTG 2 cut(s) 652, 673
Van91I CCANNNNNTGG 1 cut(s) 1207
Vha464I CTTAAG 1 cut(s) 449
XagI CCTNNNNNAGG 1 cut(s) 779
XapI RAATTY 3 cut(s) 22, 699, 744
XbaI TCTAGA 1 cut(s) 53
XceI RCATGY 2 cut(s) 382, 1177
XmiI GTMKAC 2 cut(s) 606, 672
XspI CTAG 2 cut(s) 54, 753
ZrmI AGTACT 2 cut(s) 236, 975
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.