FvH4_6g25260

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004116
Physical Location & Seq
Reverse (-)
19135050 .. 19139243
4194 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g25260.t10

Sequence Viewer

Length: 1047 bp
ATGCTTGTCTCCACAGCATCATCAACCAGTCAGCCGGATTCCTCAGCAGAAGAGTATGCCAGGGCCAATAAGGAGCTTCGCAGAATTAGCTCCTCCATGGACCTTATAAATCAGCTTAGAGCCACACACAAGGAGATTGATGGGTTGAGGTCACTCGTGGCCGAGTCTTCCCATGACAGAGACATGCTTGATATGGCAACCCATGAGTTGGATCAGGCCATCAACCATGAGAGGACGCTGCACAGTTTGTTGCTCAAGACCCTGCTCCCCAAGGATGACGCCGACGAGAGGGATTGCATATTGGAGGTTAGAGCAGGAACCGGTGGGGAGGAGGCTTCTTTGTTTGCAATGGACATATTTAAAATGTACGAGAGATTCTCACATAATAAGGGTTGGACGTTTCAAGTGTTGGATATTACTGAGTCTGATCTTAAAGGATATAAGGAAGCAAGCGCAGCAATCTCAGGGGTTGATGTTTATGGCAAACTTAAATTTGAGAGTGGTGTTCACAGAGTACAGCGAGTTCCCGTGACAGAGAAGTCTGGACGCATTCATACTAGTGCTGTTTCTGTTGCTATCCTCCCCCAAGCTGATCAGGTAGATGTCCACTTGAGGAACGAAGATCTGAAAATTGATACCTATAGATCTGGTGGTTCAGGGGGTCAGCATGCAAATACAACAAACAGTGCTGTGAGAATAACTCATATTCCAACTGGGATGACCATAGCCATACAAGATGAACGATCCCAACATATGAACAAGGCCAAGGCACTCAAGCTACTGTGTGCAAAGCTATATGAGATGGAAAGGTCTAGACTGCAGAACAATCGGTCAAAACTTCGATTAGAGCAGATTGGTAGTGGGGATCGATCAGAACGTATTCGTACGTACAACTTTCCTCAAGGTCGTGTAACTGATCATCGTGTTGGCATTACTCATCATACAATAAGTAATGTGATGCAGGGAGAGAATCTGGATGTCTTTATTGATGCCCATCTTTTGCAGCAGGAAATGGATGCCATCGCCTCTTTCAGTTCTTCCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PCRF RF-1

Protein Analysis

349

Amino Acids

38.94

Weight (kDa)

6.25

Isoelectric Point (pI)

41.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCRF PF03462 9 - 201 1.2e-59 PCRF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 107
AasI GACNNNNNNGTC 1 cut(s) 538
AccB7I CCANNNNNTGG 1 cut(s) 208
AclWI GGATC 3 cut(s) 219, 738, 873
AcoI YGGCCR 1 cut(s) 159
AcsI RAATTY 1 cut(s) 491
AcyI GRCGYC 1 cut(s) 279
AfaI GTAC 4 cut(s) 368, 516, 886, 890
AfiI CCNNNNNNNGG 2 cut(s) 208, 288
AgeI ACCGGT 1 cut(s) 320
AgsI TTSAA 1 cut(s) 404
AhlI ACTAGT 1 cut(s) 557
AjnI CCWGG 1 cut(s) 59
AluBI AGCT 6 cut(s) 76, 90, 115, 590, 778, 793
AluI AGCT 6 cut(s) 76, 90, 115, 590, 778, 793
Alw26I GTCTC 2 cut(s) 13, 174
AlwI GGATC 3 cut(s) 219, 738, 873
AoxI GGCC 4 cut(s) 63, 159, 216, 762
ApeKI GCWGC 3 cut(s) 238, 455, 1003
ApoI RAATTY 1 cut(s) 491
AsiGI ACCGGT 1 cut(s) 320
Asp700I GAANNNNTTC 1 cut(s) 879
AspLEI GCGC 1 cut(s) 455
AspS9I GGNCC 2 cut(s) 63, 100
AvaII GGWCC 1 cut(s) 100
BauI CACGAG 1 cut(s) 155
BbsI GAAGAC 1 cut(s) 159
BbvCI CCTCAGC 1 cut(s) 43
BbvI GCAGC 3 cut(s) 225, 467, 1015
BccI CCATC 5 cut(s) 134, 227, 796, 1002, 1028
BcgI CGANNNNNNTGC 2 cut(s) 809, 843
BciT130I CCWGG 1 cut(s) 61
BclI TGATCA 2 cut(s) 592, 916
BcoDI GTCTC 2 cut(s) 13, 174
BcuI ACTAGT 1 cut(s) 557
BfaI CTAG 2 cut(s) 558, 813
BfmI CTRYAG 2 cut(s) 640, 818
BglII AGATCT 2 cut(s) 622, 644
BisI GCNGC 3 cut(s) 239, 456, 1004
BlsI GCNGC 3 cut(s) 240, 457, 1005
Bme1390I CCNGG 1 cut(s) 61
Bme18I GGWCC 1 cut(s) 100
BmgT120I GGNCC 2 cut(s) 63, 100
BmiI GGNNCC 1 cut(s) 319
BmrFI CCNGG 1 cut(s) 61
BmrI ACTGGG 2 cut(s) 723, 1036
BmsI GCATC 4 cut(s) 26, 948, 979, 1006
BmuI ACTGGG 2 cut(s) 723, 1036
BpiI GAAGAC 1 cut(s) 159
BplI GAGNNNNNCTC 4 cut(s) 362, 394, 685, 717
Bpu10I CCTNAGC 1 cut(s) 43
BpuEI CTTGAG 4 cut(s) 239, 631, 758, 885
Bsa29I ATCGAT 1 cut(s) 868
BsaAI YACGTR 1 cut(s) 888
BsaBI GATNNNNATC 1 cut(s) 993
BsaHI GRCGYC 1 cut(s) 279
BsaJI CCNNGG 4 cut(s) 60, 96, 270, 765
BsaWI WCCGGW 1 cut(s) 320
Bsc4I CCNNNNNNNGG 2 cut(s) 208, 288
Bse118I RCCGGY 1 cut(s) 320
Bse1I ACTGG 3 cut(s) 27, 718, 1042
Bse3DI GCAATG 1 cut(s) 354
Bse8I GATNNNNATC 1 cut(s) 993
BseBI CCWGG 1 cut(s) 61
BseCI ATCGAT 1 cut(s) 868
BseDI CCNNGG 4 cut(s) 60, 96, 270, 765
BseGI GGATG 4 cut(s) 280, 723, 982, 1021
BseJI GATNNNNATC 1 cut(s) 993
BseLI CCNNNNNNNGG 2 cut(s) 208, 288
BseMI GCAATG 1 cut(s) 354
BseMII CTCAG 3 cut(s) 57, 411, 477
BseNI ACTGG 3 cut(s) 27, 718, 1042
BseRI GAGGAG 2 cut(s) 82, 344
BseXI GCAGC 3 cut(s) 225, 467, 1015
BsgI GTGCAG 1 cut(s) 224
BshFI GGCC 4 cut(s) 65, 161, 218, 764
BshTI ACCGGT 1 cut(s) 320
BshVI ATCGAT 1 cut(s) 868
BsiSI CCGG 2 cut(s) 35, 321
BsiWI CGTACG 1 cut(s) 884
BslI CCNNNNNNNGG 2 cut(s) 208, 288
BsmAI GTCTC 2 cut(s) 13, 174
BsmI GAATGC 1 cut(s) 549
BsnI GGCC 4 cut(s) 65, 161, 218, 764
Bsp143I GATC 9 cut(s) 211, 427, 592, 622, 644, 743, 865, 869, 916
Bsp19I CCATGG 1 cut(s) 96
BspANI GGCC 4 cut(s) 65, 161, 218, 764
BspCNI CTCAG 3 cut(s) 56, 412, 476
BspDI ATCGAT 1 cut(s) 868
BspLI GGNNCC 1 cut(s) 319
BspMAI CTGCAG 1 cut(s) 822
BspPI GGATC 3 cut(s) 219, 738, 873
BsrDI GCAATG 1 cut(s) 354
BsrFI RCCGGY 1 cut(s) 320
BsrI ACTGG 3 cut(s) 27, 718, 1042
BssAI RCCGGY 1 cut(s) 320
BssECI CCNNGG 4 cut(s) 60, 96, 270, 765
BssMI GATC 9 cut(s) 211, 427, 592, 622, 644, 743, 865, 869, 916
BssNI GRCGYC 1 cut(s) 279
BssSI CACGAG 1 cut(s) 155
BssT1I CCWWGG 3 cut(s) 96, 270, 765
Bst2BI CACGAG 1 cut(s) 155
Bst2UI CCWGG 1 cut(s) 61
Bst4CI ACNGT 3 cut(s) 245, 686, 783
Bst6I CTCTTC 1 cut(s) 45
BstACI GRCGYC 1 cut(s) 279
BstBAI YACGTR 1 cut(s) 888
BstC8I GCNNGC 2 cut(s) 451, 669
BstDEI CTNAG 4 cut(s) 43, 116, 420, 463
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 4 cut(s) 280, 723, 982, 1021
BstHHI GCGC 1 cut(s) 455
BstKTI GATC 9 cut(s) 214, 430, 595, 625, 647, 746, 868, 872, 919
BstMAI GTCTC 2 cut(s) 13, 174
BstMBI GATC 9 cut(s) 211, 427, 592, 622, 644, 743, 865, 869, 916
BstMWI GCNNNNNNNGC 2 cut(s) 87, 455
BstNI CCWGG 1 cut(s) 61
BstNSI RCATGY 2 cut(s) 187, 671
BstSCI CCNGG 1 cut(s) 59
BstSFI CTRYAG 2 cut(s) 640, 818
BstSNI TACGTA 1 cut(s) 888
BstV1I GCAGC 3 cut(s) 225, 467, 1015
BstV2I GAAGAC 1 cut(s) 159
BstX2I RGATCY 2 cut(s) 622, 644
BstYI RGATCY 2 cut(s) 622, 644
Bsu15I ATCGAT 1 cut(s) 868
BsuRI GGCC 4 cut(s) 65, 161, 218, 764
BsuTUI ATCGAT 1 cut(s) 868
BtgI CCRYGG 1 cut(s) 96
BtgZI GCGATG 1 cut(s) 1006
BtsCI GGATG 4 cut(s) 280, 723, 982, 1021
BtsIMutI CAGTG 1 cut(s) 691
Cac8I GCNNGC 2 cut(s) 451, 669
CfoI GCGC 1 cut(s) 455
Cfr10I RCCGGY 1 cut(s) 320
Cfr13I GGNCC 2 cut(s) 63, 100
ClaI ATCGAT 1 cut(s) 868
CseI GACGC 3 cut(s) 244, 287, 555
Csp6I GTAC 4 cut(s) 367, 515, 885, 889
CspAI ACCGGT 1 cut(s) 320
CviAII CATG 6 cut(s) 97, 173, 184, 203, 227, 668
CviQI GTAC 4 cut(s) 367, 515, 885, 889
DdeI CTNAG 4 cut(s) 43, 116, 420, 463
DpnI GATC 9 cut(s) 213, 429, 594, 624, 646, 745, 867, 871, 918
DpnII GATC 9 cut(s) 211, 427, 592, 622, 644, 743, 865, 869, 916
DraI TTTAAA 1 cut(s) 361
DrdI GACNNNNNNGTC 1 cut(s) 538
DseDI GACNNNNNNGTC 1 cut(s) 538
EaeI YGGCCR 1 cut(s) 159
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
Eco105I TACGTA 1 cut(s) 888
Eco130I CCWWGG 3 cut(s) 96, 270, 765
Eco47I GGWCC 1 cut(s) 100
EcoRII CCWGG 1 cut(s) 59
EcoT14I CCWWGG 3 cut(s) 96, 270, 765
ErhI CCWWGG 3 cut(s) 96, 270, 765
FaeI CATG 6 cut(s) 100, 176, 187, 206, 230, 671
FatI CATG 6 cut(s) 96, 172, 183, 202, 226, 667
FauNDI CATATG 1 cut(s) 753
FbaI TGATCA 2 cut(s) 592, 916
Fnu4HI GCNGC 3 cut(s) 239, 456, 1004
FokI GGATG 4 cut(s) 287, 730, 989, 1028
Fsp4HI GCNGC 3 cut(s) 239, 456, 1004
FspBI CTAG 2 cut(s) 558, 813
GlaI GCGC 1 cut(s) 454
GluI GCNGC 3 cut(s) 239, 456, 1004
HaeIII GGCC 4 cut(s) 65, 161, 218, 764
HapII CCGG 2 cut(s) 35, 321
HgaI GACGC 3 cut(s) 244, 287, 555
HhaI GCGC 1 cut(s) 455
Hin1I GRCGYC 1 cut(s) 279
Hin1II CATG 6 cut(s) 100, 176, 187, 206, 230, 671
Hin6I GCGC 1 cut(s) 453
HinP1I GCGC 1 cut(s) 453
HinfI GANTC 5 cut(s) 38, 164, 375, 422, 970
HpaII CCGG 2 cut(s) 35, 321
Hpy166II GTNNAC 2 cut(s) 508, 607
Hpy188I TCNGA 3 cut(s) 427, 627, 874
Hpy188III TCNNGA 4 cut(s) 256, 543, 813, 974
Hpy8I GTNNAC 2 cut(s) 508, 607
Hpy99I CGWCG 1 cut(s) 287
HpyCH4III ACNGT 3 cut(s) 245, 686, 783
HpyCH4IV ACGT 3 cut(s) 398, 877, 887
HpyCH4V TGCA 8 cut(s) 241, 297, 347, 671, 788, 820, 961, 1003
HpyF10VI GCNNNNNNNGC 2 cut(s) 87, 455
HpyF3I CTNAG 4 cut(s) 43, 116, 420, 463
HpySE526I ACGT 3 cut(s) 398, 877, 887
Hsp92I GRCGYC 1 cut(s) 279
Hsp92II CATG 6 cut(s) 100, 176, 187, 206, 230, 671
HspAI GCGC 1 cut(s) 453
Ksp22I TGATCA 2 cut(s) 592, 916
Kzo9I GATC 9 cut(s) 211, 427, 592, 622, 644, 743, 865, 869, 916
LmnI GCTCC 3 cut(s) 73, 95, 270
Lsp1109I GCAGC 3 cut(s) 225, 467, 1015
LweI GCATC 4 cut(s) 26, 948, 979, 1006
MaeI CTAG 2 cut(s) 558, 813
MaeII ACGT 3 cut(s) 398, 877, 887
MaeIII GTNAC 3 cut(s) 150, 529, 910
MalI GATC 9 cut(s) 213, 429, 594, 624, 646, 745, 867, 871, 918
MboI GATC 9 cut(s) 211, 427, 592, 622, 644, 743, 865, 869, 916
MboII GAAGA 4 cut(s) 62, 159, 632, 1029
MflI RGATCY 2 cut(s) 622, 644
MluCI AATT 3 cut(s) 84, 491, 630
MlyI GAGTC 2 cut(s) 173, 431
MmeI TCCRAC 4 cut(s) 189, 374, 390, 734
MroXI GAANNNNTTC 1 cut(s) 879
MseI TTAA 3 cut(s) 360, 432, 489
MslI CAYNNNNRTG 1 cut(s) 558
MspI CCGG 2 cut(s) 35, 321
MspR9I CCNGG 1 cut(s) 61
Mva1269I GAATGC 1 cut(s) 549
MvaI CCWGG 1 cut(s) 61
MwoI GCNNNNNNNGC 2 cut(s) 87, 455
NcoI CCATGG 1 cut(s) 96
NdeI CATATG 1 cut(s) 753
NdeII GATC 9 cut(s) 211, 427, 592, 622, 644, 743, 865, 869, 916
NlaIII CATG 6 cut(s) 100, 176, 187, 206, 230, 671
NlaIV GGNNCC 1 cut(s) 319
NmeAIII GCCGAG 1 cut(s) 187
NmuCI GTSAC 2 cut(s) 150, 529
NspI RCATGY 2 cut(s) 187, 671
PaeI GCATGC 1 cut(s) 671
PcsI WCGNNNNNNNCGW 1 cut(s) 874
PctI GAATGC 1 cut(s) 549
PdmI GAANNNNTTC 1 cut(s) 879
PfeI GAWTC 3 cut(s) 38, 375, 970
Pfl23II CGTACG 1 cut(s) 884
PflMI CCANNNNNTGG 1 cut(s) 208
PinAI ACCGGT 1 cut(s) 320
PkrI GCNGC 3 cut(s) 240, 457, 1005
PleI GAGTC 2 cut(s) 172, 430
PpsI GAGTC 2 cut(s) 172, 430
Ppu21I YACGTR 1 cut(s) 888
PsiI TTATAA 1 cut(s) 107
Psp6I CCWGG 1 cut(s) 59
PspGI CCWGG 1 cut(s) 59
PspLI CGTACG 1 cut(s) 884
PspN4I GGNNCC 1 cut(s) 319
PspPI GGNCC 2 cut(s) 63, 100
PsrI GAACNNNNNNTAC 2 cut(s) 507, 539
PstI CTGCAG 1 cut(s) 822
PsuI RGATCY 2 cut(s) 622, 644
RsaI GTAC 4 cut(s) 368, 516, 886, 890
RsaNI GTAC 4 cut(s) 367, 515, 885, 889
RseI CAYNNNNRTG 1 cut(s) 558
SaqAI TTAA 3 cut(s) 360, 432, 489
SatI GCNGC 3 cut(s) 239, 456, 1004
Sau3AI GATC 9 cut(s) 211, 427, 592, 622, 644, 743, 865, 869, 916
Sau96I GGNCC 2 cut(s) 63, 100
SchI GAGTC 2 cut(s) 173, 431
ScrFI CCNGG 1 cut(s) 61
SfaNI GCATC 4 cut(s) 26, 948, 979, 1006
SfcI CTRYAG 2 cut(s) 640, 818
SinI GGWCC 1 cut(s) 100
SmiMI CAYNNNNRTG 1 cut(s) 558
SmlI CTYRAG 4 cut(s) 254, 610, 773, 900
SmoI CTYRAG 4 cut(s) 254, 610, 773, 900
SnaBI TACGTA 1 cut(s) 888
SpeI ACTAGT 1 cut(s) 557
SphI GCATGC 1 cut(s) 671
Sse9I AATT 3 cut(s) 84, 491, 630
SspMI CTAG 2 cut(s) 558, 813
StyD4I CCNGG 1 cut(s) 59
StyI CCWWGG 3 cut(s) 96, 270, 765
TaaI ACNGT 3 cut(s) 245, 686, 783
TaiI ACGT 3 cut(s) 401, 880, 890
TaqI TCGA 2 cut(s) 841, 868
TaqII GACCGA 1 cut(s) 819
TasI AATT 3 cut(s) 84, 491, 630
TatI WGTACW 1 cut(s) 514
TfiI GAWTC 3 cut(s) 38, 375, 970
Tru1I TTAA 3 cut(s) 360, 432, 489
Tru9I TTAA 3 cut(s) 360, 432, 489
TscAI CASTG 1 cut(s) 691
TseFI GTSAC 2 cut(s) 150, 529
TseI GCWGC 3 cut(s) 238, 455, 1003
Tsp45I GTSAC 2 cut(s) 150, 529
TspDTI ATGAA 3 cut(s) 542, 753, 770
TspRI CASTG 1 cut(s) 691
Van91I CCANNNNNTGG 1 cut(s) 208
VpaK11BI GGWCC 1 cut(s) 100
XapI RAATTY 1 cut(s) 491
XbaI TCTAGA 1 cut(s) 812
XceI RCATGY 2 cut(s) 187, 671
XmnI GAANNNNTTC 1 cut(s) 879
XspI CTAG 2 cut(s) 558, 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.