Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004116

Physical Location & Seq

Genomic Coordinates Forward (+)

1038086 .. 1041498

Gene Structure 3413 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1002600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1077 bp

ATGGAGCAAAGGCTATCGCTTATCGAGCAGAGGAGTGGTTTTCTTCACACTCTCATTAACCAGCCTGATGCCTCTGCAGAAGAGTATGCGAGGGCGAACAAGGAGCTACGGAAACTCAGCACTTCCGTGGACCTTATAAGTCGTCTAAGAGCCACAACCAAGGAGATTAATGGTTTGAGGACTCTCATCGATGAATGTTCTCAAGAAAAAGACATGCTTGATATGGTAACTACCGAATTGGATCAAGCCATCCACGAAGAAAGAAGGCTGCACAATTTGTTGCTCAAGTCCCTGCTTCCTAAGGATGATGCTGATGAGAGGGATTGCATATTAGAGGTCAGAGCAGGAACTGGTGGGGACGAGGCTTCTTTGTTTGCAATGGACATATTTAAAATGTATGATAGATTCTCACACAAGAAGGGCTGGAAGTTTGAAGTGGTGGATATAACTGAGTCTGATCTTAAAGGATATAAGGAAGCTACTGCAGCGATCTCAGGAGCTGATGTTTATGGGAAACTTAAATTTGAGAGTGGGGTTCACAGAGTACAGCGAGTTCCTGTGACAGAGAAGTCTGGACGTATTCACACTAGTGCTGTTTCTGTTGCTATCCTTCCTCAAGCTGATGAGGTAGATGTCCACCTGAGGAACGAAGATTTGAGAATTGACACGTATAGATCTGGTGGTTCAGGTGGTCAACATGCAAATACAACTAACAGTGCTGTTAGAATAACTCATATTCCAACTGGCATGACTGTAGCCATACAAGACGAGCGATCCCAACATATGAACAAAGCCAAGGCACTTAAGGTGTTATGTGCAAAGCTTTATGAGATAGAGAGGTCTAGACTTCACGCCAATCGAACAAAACTTCGATCTCAACAGATTGGTAGTGGGGATAGATCTGAACGCATCCGTACATACAATTTTCCTCAAGGACGTGTAACGGATCACCGTGTTGGCATCACTCATCATTCGATAAATGATGTGATGCAGGGAGAGAATCTGGATGTATTTATTGATGCCCTTCTTTTGCAAGAAGAAATGGATGCAATTGCTTCTTTCAGTTCTTCGCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PCRF RF-1

Protein Analysis

359

Amino Acids

40.3

Weight (kDa)

6.18

Isoelectric Point (pI)

43.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PCRF	PF03462	11 - 201	3.1e-60	PCRF domain
RF-1	PF00472	208 - 318	6.7e-40	RF-1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004116)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47020 AT2G47020 AT2G47020 AT2G47020 AT2G47020
fragaria_vesca	FvH4_6g25260 FvH4_6g25260 FvH4_6g25260 FvH4_6g25260 FvH4_6g25260 FvH4_6g25260 FvH4_6g25260 FvH4_6g25260 FvH4_6g25260
malus_domestica	MD05G1002600.v1.1
prunus_persica	Prupe.7G036500_v2.0.a1 Prupe.7G036500_v2.0.a1 Prupe.7G036500_v2.0.a1 Prupe.7G036500_v2.0.a1 Prupe.7G036500_v2.0.a1 Prupe.7G036500_v2.0.a1 Prupe.7G036500_v2.0.a1
pyrus_communis	pycom05g00250
rosa_chinensis	RchiOBHm_Chr2g0125161
rosa_laevigata	RLG00000018851
rosa_multiflora	Rmu_sc0003505.1_g000032
rosa_roxburghii	Rroxscaffold_2G00118220 Rroxscaffold_2G00118690
rosa_rugosa	Rorug02G0256000
rosa_samantha	Rh2AG314300 Rh2BG322500 Rh2DG340900
rosa_wichuraiana	Rw2G025430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	137
AasI	GACNNNNNNGTC	1 cut(s)	568
AclWI	GGATC	3 cut(s)	249, 768, 954
AcsI	RAATTY	1 cut(s)	521
AfaI	GTAC	2 cut(s)	546, 916
AflII	CTTAAG	1 cut(s)	803
AflIII	ACRYGT	2 cut(s)	666, 937
AgsI	TTSAA	1 cut(s)	434
AhlI	ACTAGT	1 cut(s)	587
AjiI	CACGTC	1 cut(s)	938
AleI	CACNNNNGTG	2 cut(s)	125, 588
AluBI	AGCT	5 cut(s)	106, 479, 500, 620, 823
AluI	AGCT	5 cut(s)	106, 479, 500, 620, 823
AlwI	GGATC	3 cut(s)	249, 768, 954
AlwNI	CAGNNNCTG	2 cut(s)	350, 500
ApeKI	GCWGC	2 cut(s)	268, 485
ApoI	RAATTY	1 cut(s)	521
AseI	ATTAAT	1 cut(s)	168
AspS9I	GGNCC	1 cut(s)	130
AsuHPI	GGTGA	1 cut(s)	941
AvaII	GGWCC	1 cut(s)	130
AxyI	CCTNAGG	2 cut(s)	300, 641
BbvI	GCAGC	2 cut(s)	255, 497
BccI	CCATC	1 cut(s)	257
BcuI	ACTAGT	1 cut(s)	587
BfaI	CTAG	2 cut(s)	588, 843
BfmI	CTRYAG	3 cut(s)	75, 483, 753
BfrI	CTTAAG	1 cut(s)	803
BglII	AGATCT	2 cut(s)	674, 899
BisI	GCNGC	2 cut(s)	269, 486
BlsI	GCNGC	2 cut(s)	270, 487
Bme18I	GGWCC	1 cut(s)	130
BmgBI	CACGTC	1 cut(s)	938
BmgT120I	GGNCC	1 cut(s)	130
BmsI	GCATC	7 cut(s)	58, 298, 918, 969, 978, 1009, 1036
BpuEI	CTTGAG	4 cut(s)	186, 269, 600, 915
Bsa29I	ATCGAT	1 cut(s)	189
BsaAI	YACGTR	1 cut(s)	669
BsaJI	CCNNGG	3 cut(s)	126, 159, 795
BsaXI	ACNNNNNCTCC	2 cut(s)	489, 519
Bse1I	ACTGG	2 cut(s)	355, 748
Bse21I	CCTNAGG	2 cut(s)	300, 641
Bse3DI	GCAATG	1 cut(s)	384
BseCI	ATCGAT	1 cut(s)	189
BseDI	CCNNGG	3 cut(s)	126, 159, 795
BseGI	GGATG	5 cut(s)	249, 310, 909, 1012, 1051
BseMI	GCAATG	1 cut(s)	384
BseMII	CTCAG	4 cut(s)	130, 441, 507, 632
BseNI	ACTGG	2 cut(s)	355, 748
BseRI	GAGGAG	1 cut(s)	46
BseXI	GCAGC	2 cut(s)	255, 497
BsgI	GTGCAG	1 cut(s)	254
BshVI	ATCGAT	1 cut(s)	189
BslFI	GGGAC	2 cut(s)	274, 371
BsmFI	GGGAC	2 cut(s)	274, 371
Bsp143I	GATC	8 cut(s)	241, 457, 489, 674, 773, 872, 899, 946
BspCNI	CTCAG	4 cut(s)	129, 442, 506, 633
BspDI	ATCGAT	1 cut(s)	189
BspMAI	CTGCAG	2 cut(s)	79, 487
BspPI	GGATC	3 cut(s)	249, 768, 954
BspTI	CTTAAG	1 cut(s)	803
BsrDI	GCAATG	1 cut(s)	384
BsrI	ACTGG	2 cut(s)	355, 748
BssECI	CCNNGG	3 cut(s)	126, 159, 795
BssMI	GATC	8 cut(s)	241, 457, 489, 674, 773, 872, 899, 946
BssT1I	CCWWGG	2 cut(s)	159, 795
Bst4CI	ACNGT	3 cut(s)	716, 754, 953
Bst6I	CTCTTC	1 cut(s)	75
BstAFI	CTTAAG	1 cut(s)	803
BstBAI	YACGTR	1 cut(s)	669
BstDEI	CTNAG	6 cut(s)	116, 146, 300, 450, 493, 641
BstDSI	CCRYGG	1 cut(s)	126
BstF5I	GGATG	5 cut(s)	249, 310, 909, 1012, 1051
BstKTI	GATC	8 cut(s)	244, 460, 492, 677, 776, 875, 902, 949
BstMBI	GATC	8 cut(s)	241, 457, 489, 674, 773, 872, 899, 946
BstMWI	GCNNNNNNNGC	2 cut(s)	25, 485
BstNSI	RCATGY	2 cut(s)	217, 701
BstSFI	CTRYAG	3 cut(s)	75, 483, 753
BstV1I	GCAGC	2 cut(s)	255, 497
BstX2I	RGATCY	2 cut(s)	674, 899
BstYI	RGATCY	2 cut(s)	674, 899
Bsu15I	ATCGAT	1 cut(s)	189
Bsu36I	CCTNAGG	2 cut(s)	300, 641
BsuTUI	ATCGAT	1 cut(s)	189
BtgI	CCRYGG	1 cut(s)	126
BtrI	CACGTC	1 cut(s)	938
BtsCI	GGATG	5 cut(s)	249, 310, 909, 1012, 1051
BtsIMutI	CAGTG	1 cut(s)	721
CaiI	CAGNNNCTG	2 cut(s)	350, 500
Cfr13I	GGNCC	1 cut(s)	130
ClaI	ATCGAT	1 cut(s)	189
Csp6I	GTAC	2 cut(s)	545, 915
CviAII	CATG	3 cut(s)	214, 698, 748
CviQI	GTAC	2 cut(s)	545, 915
DdeI	CTNAG	6 cut(s)	116, 146, 300, 450, 493, 641
DpnI	GATC	8 cut(s)	243, 459, 491, 676, 775, 874, 901, 948
DpnII	GATC	8 cut(s)	241, 457, 489, 674, 773, 872, 899, 946
DraI	TTTAAA	1 cut(s)	391
DrdI	GACNNNNNNGTC	1 cut(s)	568
DseDI	GACNNNNNNGTC	1 cut(s)	568
Eam1104I	CTCTTC	1 cut(s)	75
EarI	CTCTTC	1 cut(s)	75
Eco130I	CCWWGG	2 cut(s)	159, 795
Eco47I	GGWCC	1 cut(s)	130
Eco81I	CCTNAGG	2 cut(s)	300, 641
EcoT14I	CCWWGG	2 cut(s)	159, 795
ErhI	CCWWGG	2 cut(s)	159, 795
FaeI	CATG	3 cut(s)	217, 701, 751
FalI	AAGNNNNNCTT	2 cut(s)	201, 233
FaqI	GGGAC	2 cut(s)	274, 371
FatI	CATG	3 cut(s)	213, 697, 747
FauNDI	CATATG	1 cut(s)	783
Fnu4HI	GCNGC	2 cut(s)	269, 486
FokI	GGATG	5 cut(s)	236, 317, 896, 1019, 1058
Fsp4HI	GCNGC	2 cut(s)	269, 486
FspBI	CTAG	2 cut(s)	588, 843
GluI	GCNGC	2 cut(s)	269, 486
Hin1II	CATG	3 cut(s)	217, 701, 751
HincII	GTYRAC	1 cut(s)	695
HindII	GTYRAC	1 cut(s)	695
HindIII	AAGCTT	1 cut(s)	821
HinfI	GANTC	4 cut(s)	181, 405, 452, 1000
HphI	GGTGA	1 cut(s)	941
Hpy166II	GTNNAC	4 cut(s)	130, 538, 637, 695
Hpy188I	TCNGA	3 cut(s)	341, 457, 904
Hpy188III	TCNNGA	5 cut(s)	203, 495, 573, 843, 1004
Hpy8I	GTNNAC	4 cut(s)	130, 538, 637, 695
HpyAV	CCTTC	4 cut(s)	258, 412, 620, 1034
HpyCH4III	ACNGT	3 cut(s)	716, 754, 953
HpyCH4IV	ACGT	3 cut(s)	577, 668, 937
HpyF10VI	GCNNNNNNNGC	2 cut(s)	25, 485
HpyF3I	CTNAG	6 cut(s)	116, 146, 300, 450, 493, 641
HpySE526I	ACGT	3 cut(s)	577, 668, 937
Hsp92II	CATG	3 cut(s)	217, 701, 751
Kzo9I	GATC	8 cut(s)	241, 457, 489, 674, 773, 872, 899, 946
LmnI	GCTCC	3 cut(s)	4, 103, 497
Lsp1109I	GCAGC	2 cut(s)	255, 497
LweI	GCATC	7 cut(s)	58, 298, 918, 969, 978, 1009, 1036
MaeI	CTAG	2 cut(s)	588, 843
MaeII	ACGT	3 cut(s)	577, 668, 937
MaeIII	GTNAC	3 cut(s)	226, 559, 940
MalI	GATC	8 cut(s)	243, 459, 491, 676, 775, 874, 901, 948
MboI	GATC	8 cut(s)	241, 457, 489, 674, 773, 872, 899, 946
MboII	GAAGA	6 cut(s)	35, 92, 269, 662, 1049, 1059
MfeI	CAATTG	1 cut(s)	1050
MflI	RGATCY	2 cut(s)	674, 899
MluCI	AATT	6 cut(s)	236, 274, 521, 660, 922, 1050
MlyI	GAGTC	2 cut(s)	175, 461
MmeI	TCCRAC	1 cut(s)	764
MseI	TTAA	6 cut(s)	57, 168, 390, 462, 519, 804
MslI	CAYNNNNRTG	2 cut(s)	125, 588
MspCI	CTTAAG	1 cut(s)	803
MunI	CAATTG	1 cut(s)	1050
MwoI	GCNNNNNNNGC	2 cut(s)	25, 485
NdeI	CATATG	1 cut(s)	783
NdeII	GATC	8 cut(s)	241, 457, 489, 674, 773, 872, 899, 946
NlaIII	CATG	3 cut(s)	217, 701, 751
NmuCI	GTSAC	1 cut(s)	559
NspI	RCATGY	2 cut(s)	217, 701
OliI	CACNNNNGTG	2 cut(s)	125, 588
PfeI	GAWTC	2 cut(s)	405, 1000
PkrI	GCNGC	2 cut(s)	270, 487
PleI	GAGTC	2 cut(s)	175, 460
PpsI	GAGTC	2 cut(s)	175, 460
Ppu21I	YACGTR	1 cut(s)	669
PshBI	ATTAAT	1 cut(s)	168
PsiI	TTATAA	1 cut(s)	137
PspPI	GGNCC	1 cut(s)	130
PsrI	GAACNNNNNNTAC	2 cut(s)	537, 569
PstI	CTGCAG	2 cut(s)	79, 487
PstNI	CAGNNNCTG	2 cut(s)	350, 500
PsuI	RGATCY	2 cut(s)	674, 899
RsaI	GTAC	2 cut(s)	546, 916
RsaNI	GTAC	2 cut(s)	545, 915
RseI	CAYNNNNRTG	2 cut(s)	125, 588
SaqAI	TTAA	6 cut(s)	57, 168, 390, 462, 519, 804
SatI	GCNGC	2 cut(s)	269, 486
Sau3AI	GATC	8 cut(s)	241, 457, 489, 674, 773, 872, 899, 946
Sau96I	GGNCC	1 cut(s)	130
SchI	GAGTC	2 cut(s)	175, 461
SfaNI	GCATC	7 cut(s)	58, 298, 918, 969, 978, 1009, 1036
SfcI	CTRYAG	3 cut(s)	75, 483, 753
SinI	GGWCC	1 cut(s)	130
SmiMI	CAYNNNNRTG	2 cut(s)	125, 588
SmlI	CTYRAG	5 cut(s)	201, 284, 615, 803, 930
SmoI	CTYRAG	5 cut(s)	201, 284, 615, 803, 930
SpeI	ACTAGT	1 cut(s)	587
Sse9I	AATT	6 cut(s)	236, 274, 521, 660, 922, 1050
SspMI	CTAG	2 cut(s)	588, 843
StyI	CCWWGG	2 cut(s)	159, 795
TaaI	ACNGT	3 cut(s)	716, 754, 953
TaiI	ACGT	3 cut(s)	580, 671, 940
TaqI	TCGA	5 cut(s)	24, 189, 859, 871, 974
TasI	AATT	6 cut(s)	236, 274, 521, 660, 922, 1050
TatI	WGTACW	1 cut(s)	544
TfiI	GAWTC	2 cut(s)	405, 1000
Tru1I	TTAA	6 cut(s)	57, 168, 390, 462, 519, 804
Tru9I	TTAA	6 cut(s)	57, 168, 390, 462, 519, 804
TscAI	CASTG	1 cut(s)	721
TseFI	GTSAC	1 cut(s)	559
TseI	GCWGC	2 cut(s)	268, 485
Tsp45I	GTSAC	1 cut(s)	559
TspDTI	ATGAA	2 cut(s)	207, 800
TspGWI	ACGGA	4 cut(s)	115, 124, 902, 959
TspRI	CASTG	1 cut(s)	721
Vha464I	CTTAAG	1 cut(s)	803
VpaK11BI	GGWCC	1 cut(s)	130
VspI	ATTAAT	1 cut(s)	168
XapI	RAATTY	1 cut(s)	521
XbaI	TCTAGA	1 cut(s)	842
XceI	RCATGY	2 cut(s)	217, 701
XspI	CTAG	2 cut(s)	588, 843

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.