AT3G01800

Ribosome-recycling factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007948
Physical Location & Seq
Forward (+)
285871 .. 287950
2080 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G01800.1

Sequence Viewer

Length: 804 bp
ATGATGGCTATGTTCGTGAGACGAGCTCTATCTAGTCGTAACGCGATTGGGTTTCTCAGAAGAAGCTCCGTTGTTGTTCAATCTCGTGATTTCTCCTTCATTTCTTCACCCAATCTCATGATGATGGGAGGCCGAGATCTGCAATTCGATTTGTCCATGCCCGATTTCCTCAGAGATTCTCGTCGAGGCTTTGCAAAAGGCAAAAAATCAAAGGACGATTCAGGAACAGGGATGGTCGACGCAGCTCCAGATATCGGACCTACCGTGAAAGCAGCAGCCTCTTCTCAAATGGAGGCAGCCATTGATGCATTGTCCCGGGATTTAACTAAATTAAGAACCGGAAGAGCCGCTCCAGGAATGCTTGATCATATTGTTGTTGAAACGGGAGGAGTAAAGATGCCACTCAATCACTTAGCTTTGGTCTCTGTCTTGGATCCTAAGACTCTCTCTGTCAATCCTTATGATCCCGATACGGTCAAAGAACTTGAGAAAGCAATAGTCGCGTCGCCATTAGGATTAAACCCGAAACTGGATGGGCAACGACTGGTCGCATCTATCCCAGCATTGACCAAGGAGCATATACAGGCAATGTGCAAGATTGTTACAAAGTCCAGTGAAGTTGTCAAACAAAGCATAAGAAGAGCGAGGCAAAAGGCACTGGACACGATAAAGAAAGCAGGCTCGAGTCTACCAAAGGATGAAGTAAAAAGACTGGAGAAGGAAGTAGATGAGTTGACGAAGAAATTTGTGAAGTCAGCAGAGGACATGTGCAAGTCCAAAGAGAAAGAGATCACCGAAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRF

Protein Analysis

267

Amino Acids

29.17

Weight (kDa)

9.63

Isoelectric Point (pI)

48.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRF PF01765 105 - 264 8.7e-52 Ribosome recycling factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 350
AccI GTMKAC 2 cut(s) 237, 688
AccII CGCG 2 cut(s) 44, 503
AciI CCGC 1 cut(s) 348
AclWI GGATC 3 cut(s) 428, 441, 458
AcsI RAATTY 1 cut(s) 743
AfiI CCNNNNNNNGG 2 cut(s) 254, 529
AflIII ACRYGT 1 cut(s) 765
AgsI TTSAA 2 cut(s) 80, 380
AjnI CCWGG 1 cut(s) 352
AluBI AGCT 4 cut(s) 26, 66, 245, 416
AluI AGCT 4 cut(s) 26, 66, 245, 416
Alw21I GWGCWC 1 cut(s) 28
Alw26I GTCTC 2 cut(s) 13, 427
AlwI GGATC 3 cut(s) 428, 441, 458
Ama87I CYCGRG 2 cut(s) 315, 682
AoxI GGCC 1 cut(s) 130
ApeKI GCWGC 4 cut(s) 242, 272, 275, 296
ApoI RAATTY 1 cut(s) 743
AspS9I GGNCC 1 cut(s) 257
AsuC2I CCSGG 2 cut(s) 316, 317
AsuHPI GGTGA 2 cut(s) 99, 784
AvaI CYCGRG 2 cut(s) 315, 682
AvaII GGWCC 1 cut(s) 257
BamHI GGATCC 1 cut(s) 433
BanII GRGCYC 1 cut(s) 28
BauI CACGAG 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 28
BbvI GCAGC 4 cut(s) 254, 284, 287, 308
BccI CCATC 3 cut(s) 118, 226, 527
BciT130I CCWGG 1 cut(s) 354
BclI TGATCA 1 cut(s) 364
BcnI CCSGG 2 cut(s) 316, 317
BcoDI GTCTC 2 cut(s) 13, 427
BfaI CTAG 1 cut(s) 33
BglII AGATCT 1 cut(s) 136
BisI GCNGC 5 cut(s) 243, 273, 276, 297, 348
BlsI GCNGC 5 cut(s) 244, 274, 277, 298, 349
Bme1390I CCNGG 3 cut(s) 316, 317, 354
Bme18I GGWCC 1 cut(s) 257
BmeT110I CYCGRG 2 cut(s) 315, 682
BmgT120I GGNCC 1 cut(s) 257
BmiI GGNNCC 1 cut(s) 435
BmrFI CCNGG 3 cut(s) 316, 317, 354
BmsI GCATC 3 cut(s) 295, 387, 560
BplI GAGNNNNNCTC 2 cut(s) 10, 42
BpmI CTGGAG 3 cut(s) 231, 336, 734
BpuEI CTTGAG 1 cut(s) 506
BpuMI CCSGG 2 cut(s) 316, 317
BsaI GGTCTC 1 cut(s) 427
BsaJI CCNNGG 2 cut(s) 315, 570
BsaWI WCCGGW 1 cut(s) 338
Bsc4I CCNNNNNNNGG 2 cut(s) 254, 529
Bse1I ACTGG 5 cut(s) 534, 549, 612, 663, 717
Bse3DI GCAATG 1 cut(s) 594
BseBI CCWGG 1 cut(s) 354
BseDI CCNNGG 2 cut(s) 315, 570
BseGI GGATG 3 cut(s) 237, 538, 703
BseLI CCNNNNNNNGG 2 cut(s) 254, 529
BseMI GCAATG 1 cut(s) 594
BseMII CTCAG 2 cut(s) 70, 184
BseNI ACTGG 5 cut(s) 534, 549, 612, 663, 717
BseRI GAGGAG 1 cut(s) 402
BseXI GCAGC 4 cut(s) 254, 284, 287, 308
BseYI CCCAGC 1 cut(s) 559
Bsh1236I CGCG 2 cut(s) 44, 503
BshFI GGCC 1 cut(s) 132
BsiHKAI GWGCWC 1 cut(s) 28
BsiHKCI CYCGRG 2 cut(s) 315, 682
BsiSI CCGG 2 cut(s) 316, 339
BslFI GGGAC 1 cut(s) 298
BslI CCNNNNNNNGG 2 cut(s) 254, 529
BsmAI GTCTC 2 cut(s) 13, 427
BsmBI CGTCTC 1 cut(s) 13
BsmFI GGGAC 1 cut(s) 298
BsmI GAATGC 1 cut(s) 363
BsnI GGCC 1 cut(s) 132
Bso31I GGTCTC 1 cut(s) 427
BsoBI CYCGRG 2 cut(s) 315, 682
Bsp1286I GDGCHC 1 cut(s) 28
Bsp143I GATC 5 cut(s) 136, 364, 433, 463, 789
BspACI CCGC 1 cut(s) 348
BspANI GGCC 1 cut(s) 132
BspCNI CTCAG 2 cut(s) 69, 183
BspFNI CGCG 2 cut(s) 44, 503
BspHI TCATGA 1 cut(s) 117
BspLI GGNNCC 1 cut(s) 435
BspPI GGATC 3 cut(s) 428, 441, 458
BspQI GCTCTTC 2 cut(s) 337, 634
BspTNI GGTCTC 1 cut(s) 427
BsrBI CCGCTC 1 cut(s) 350
BsrDI GCAATG 1 cut(s) 594
BsrI ACTGG 5 cut(s) 534, 549, 612, 663, 717
BssECI CCNNGG 2 cut(s) 315, 570
BssMI GATC 5 cut(s) 136, 364, 433, 463, 789
BssSI CACGAG 1 cut(s) 84
BssT1I CCWWGG 1 cut(s) 570
Bst2BI CACGAG 1 cut(s) 84
Bst2UI CCWGG 1 cut(s) 354
Bst4CI ACNGT 2 cut(s) 265, 475
Bst6I CTCTTC 3 cut(s) 286, 337, 634
BstC8I GCNNGC 1 cut(s) 679
BstDEI CTNAG 4 cut(s) 56, 170, 412, 438
BstF5I GGATG 3 cut(s) 237, 538, 703
BstFNI CGCG 2 cut(s) 44, 503
BstKTI GATC 5 cut(s) 139, 367, 436, 466, 792
BstMAI GTCTC 2 cut(s) 13, 427
BstMBI GATC 5 cut(s) 136, 364, 433, 463, 789
BstMWI GCNNNNNNNGC 2 cut(s) 305, 500
BstNI CCWGG 1 cut(s) 354
BstNSI RCATGY 1 cut(s) 769
BstSCI CCNGG 3 cut(s) 314, 315, 352
BstUI CGCG 2 cut(s) 44, 503
BstV1I GCAGC 4 cut(s) 254, 284, 287, 308
BstX2I RGATCY 2 cut(s) 136, 433
BstYI RGATCY 2 cut(s) 136, 433
BsuRI GGCC 1 cut(s) 132
BtsCI GGATG 3 cut(s) 237, 538, 703
BtsIMutI CAGTG 2 cut(s) 619, 656
Cac8I GCNNGC 1 cut(s) 679
CciI TCATGA 1 cut(s) 117
Cfr13I GGNCC 1 cut(s) 257
Cfr9I CCCGGG 1 cut(s) 315
CseI GACGC 2 cut(s) 248, 492
CviAII CATG 3 cut(s) 118, 157, 766
DdeI CTNAG 4 cut(s) 56, 170, 412, 438
DpnI GATC 5 cut(s) 138, 366, 435, 465, 791
DpnII GATC 5 cut(s) 136, 364, 433, 463, 789
Eam1104I CTCTTC 3 cut(s) 286, 337, 634
EarI CTCTTC 3 cut(s) 286, 337, 634
Ecl136II GAGCTC 1 cut(s) 26
Eco130I CCWWGG 1 cut(s) 570
Eco24I GRGCYC 1 cut(s) 28
Eco31I GGTCTC 1 cut(s) 427
Eco32I GATATC 1 cut(s) 253
Eco47I GGWCC 1 cut(s) 257
Eco53kI GAGCTC 1 cut(s) 26
Eco88I CYCGRG 2 cut(s) 315, 682
EcoICRI GAGCTC 1 cut(s) 26
EcoRII CCWGG 1 cut(s) 352
EcoRV GATATC 1 cut(s) 253
EcoT14I CCWWGG 1 cut(s) 570
EcoT22I ATGCAT 1 cut(s) 310
EcoT38I GRGCYC 1 cut(s) 28
ErhI CCWWGG 1 cut(s) 570
Esp3I CGTCTC 1 cut(s) 13
FaeI CATG 3 cut(s) 121, 160, 769
FaiI YATR 9 cut(s) 11, 119, 158, 369, 462, 579, 581, 635, 767
FaqI GGGAC 1 cut(s) 298
FatI CATG 3 cut(s) 117, 156, 765
FbaI TGATCA 1 cut(s) 364
FblI GTMKAC 2 cut(s) 237, 688
Fnu4HI GCNGC 5 cut(s) 243, 273, 276, 297, 348
FokI GGATG 3 cut(s) 244, 545, 710
FriOI GRGCYC 1 cut(s) 28
Fsp4HI GCNGC 5 cut(s) 243, 273, 276, 297, 348
FspBI CTAG 1 cut(s) 33
GluI GCNGC 5 cut(s) 243, 273, 276, 297, 348
GsaI CCCAGC 1 cut(s) 563
GsuI CTGGAG 3 cut(s) 231, 336, 734
HaeIII GGCC 1 cut(s) 132
HapII CCGG 2 cut(s) 316, 339
HgaI GACGC 2 cut(s) 248, 492
Hin1II CATG 3 cut(s) 121, 160, 769
HincII GTYRAC 2 cut(s) 238, 735
HindII GTYRAC 2 cut(s) 238, 735
HinfI GANTC 4 cut(s) 176, 218, 442, 685
HpaII CCGG 2 cut(s) 316, 339
HphI GGTGA 2 cut(s) 99, 784
Hpy166II GTNNAC 3 cut(s) 238, 689, 735
Hpy188I TCNGA 3 cut(s) 59, 173, 257
Hpy188III TCNNGA 6 cut(s) 16, 86, 118, 222, 248, 467
Hpy8I GTNNAC 3 cut(s) 238, 689, 735
Hpy99I CGWCG 3 cut(s) 186, 242, 508
HpyAV CCTTC 2 cut(s) 106, 712
HpyCH4III ACNGT 2 cut(s) 265, 475
HpyCH4V TGCA 5 cut(s) 142, 194, 308, 594, 771
HpyF10VI GCNNNNNNNGC 2 cut(s) 305, 500
HpyF3I CTNAG 4 cut(s) 56, 170, 412, 438
Hsp92II CATG 3 cut(s) 121, 160, 769
Ksp22I TGATCA 1 cut(s) 364
Kzo9I GATC 5 cut(s) 136, 364, 433, 463, 789
LguI GCTCTTC 2 cut(s) 337, 634
LmnI GCTCC 4 cut(s) 71, 250, 355, 574
Lsp1109I GCAGC 4 cut(s) 254, 284, 287, 308
LweI GCATC 3 cut(s) 295, 387, 560
MaeI CTAG 1 cut(s) 33
MaeIII GTNAC 2 cut(s) 38, 601
MalI GATC 5 cut(s) 138, 366, 435, 465, 791
MbiI CCGCTC 1 cut(s) 350
MboI GATC 5 cut(s) 136, 364, 433, 463, 789
MboII GAAGA 6 cut(s) 72, 96, 273, 354, 651, 751
MflI RGATCY 2 cut(s) 136, 433
MhlI GDGCHC 1 cut(s) 28
MluCI AATT 3 cut(s) 143, 329, 743
MlyI GAGTC 2 cut(s) 436, 694
MnlI CCTC 8 cut(s) 122, 179, 179, 286, 289, 380, 639, 754
Mph1103I ATGCAT 1 cut(s) 310
MseI TTAA 3 cut(s) 323, 332, 518
MslI CAYNNNNRTG 1 cut(s) 122
MspI CCGG 2 cut(s) 316, 339
MspR9I CCNGG 3 cut(s) 316, 317, 354
Mva1269I GAATGC 1 cut(s) 363
MvaI CCWGG 1 cut(s) 354
MvnI CGCG 2 cut(s) 44, 503
MwoI GCNNNNNNNGC 2 cut(s) 305, 500
NciI CCSGG 2 cut(s) 316, 317
NdeII GATC 5 cut(s) 136, 364, 433, 463, 789
NlaIII CATG 3 cut(s) 121, 160, 769
NlaIV GGNNCC 1 cut(s) 435
NmeAIII GCCGAG 1 cut(s) 158
NsiI ATGCAT 1 cut(s) 310
NspI RCATGY 1 cut(s) 769
PaeR7I CTCGAG 1 cut(s) 682
PagI TCATGA 1 cut(s) 117
PciI ACATGT 1 cut(s) 765
PciSI GCTCTTC 2 cut(s) 337, 634
PcsI WCGNNNNNNNCGW 1 cut(s) 261
PctI GAATGC 1 cut(s) 363
PfeI GAWTC 2 cut(s) 176, 218
PfoI TCCNGGA 1 cut(s) 352
PkrI GCNGC 5 cut(s) 244, 274, 277, 298, 349
PleI GAGTC 2 cut(s) 436, 693
PpsI GAGTC 2 cut(s) 436, 693
PscI ACATGT 1 cut(s) 765
Psp124BI GAGCTC 1 cut(s) 28
Psp6I CCWGG 1 cut(s) 352
PspFI CCCAGC 1 cut(s) 559
PspGI CCWGG 1 cut(s) 352
PspN4I GGNNCC 1 cut(s) 435
PspPI GGNCC 1 cut(s) 257
PspXI VCTCGAGB 1 cut(s) 682
PsuI RGATCY 2 cut(s) 136, 433
RseI CAYNNNNRTG 1 cut(s) 122
SacI GAGCTC 1 cut(s) 28
SalI GTCGAC 1 cut(s) 236
SapI GCTCTTC 2 cut(s) 337, 634
SaqAI TTAA 3 cut(s) 323, 332, 518
SatI GCNGC 5 cut(s) 243, 273, 276, 297, 348
Sau3AI GATC 5 cut(s) 136, 364, 433, 463, 789
Sau96I GGNCC 1 cut(s) 257
SchI GAGTC 2 cut(s) 436, 694
ScrFI CCNGG 3 cut(s) 316, 317, 354
SduI GDGCHC 1 cut(s) 28
SetI ASST 5 cut(s) 28, 68, 247, 262, 418
SfaNI GCATC 3 cut(s) 295, 387, 560
Sfr274I CTCGAG 1 cut(s) 682
SinI GGWCC 1 cut(s) 257
SlaI CTCGAG 1 cut(s) 682
SmaI CCCGGG 1 cut(s) 317
SmiMI CAYNNNNRTG 1 cut(s) 122
SmlI CTYRAG 2 cut(s) 485, 682
SmoI CTYRAG 2 cut(s) 485, 682
Sse9I AATT 3 cut(s) 143, 329, 743
SsiI CCGC 1 cut(s) 348
SspMI CTAG 1 cut(s) 33
SstI GAGCTC 1 cut(s) 28
StyD4I CCNGG 3 cut(s) 314, 315, 352
StyI CCWWGG 1 cut(s) 570
TaaI ACNGT 2 cut(s) 265, 475
TaqI TCGA 4 cut(s) 147, 184, 237, 683
TasI AATT 3 cut(s) 143, 329, 743
TauI GCSGC 1 cut(s) 350
TfiI GAWTC 2 cut(s) 176, 218
Tru1I TTAA 3 cut(s) 323, 332, 518
Tru9I TTAA 3 cut(s) 323, 332, 518
TscAI CASTG 2 cut(s) 619, 663
TseI GCWGC 4 cut(s) 242, 272, 275, 296
TspDTI ATGAA 2 cut(s) 88, 714
TspGWI ACGGA 1 cut(s) 58
TspMI CCCGGG 1 cut(s) 315
TspRI CASTG 2 cut(s) 619, 663
VpaK11BI GGWCC 1 cut(s) 257
XapI RAATTY 1 cut(s) 743
XceI RCATGY 1 cut(s) 769
XhoI CTCGAG 1 cut(s) 682
XmaI CCCGGG 1 cut(s) 315
XmiI GTMKAC 2 cut(s) 237, 688
XspI CTAG 1 cut(s) 33
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.