RLG00000022301

Ribosome-recycling factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0007948
Physical Location & Seq
Forward (+)
82389280 .. 82391240
1961 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022301

Sequence Viewer

Length: 774 bp
ATGGCGATCTTTCTCCGACGCGGATTCTCAATCCGAAACGCGCTTCTCCGACGAAGCTCAGCTCTGTACAGCTGCGATCTCCCGCTCTGCAGCTCCGAGACGCCGCAGAGGATTCTCTCTCTTCCGGCTCTTGAATTTGTGAAAGAAAGTCGCAGAGGCTTCGCCAAAGGCCGTAAATCAAAAGATGAATCTTCTGGAACTACAATGGAGGTTTTACCTGATATGGGACCCACCATCAAGGCCAATGCGACCTCACATATGGAGGCTGCAATTGCGGCATTGTCAGTAGAACTGAGCAAACTACGCACCGGAAGAGCATCACCAGGAATGCTGGACCACATTATTGTGGAAACCAGTGGGTTGAAGCTGCCCTTGCATCAGATAGCGGTTGTTTCAGTCATGGATTCAAAAACGCTATCGATCAATCCGTTTGATCCAAGTGCCTTGAAAAATATAGAGACTGCCATTGTTGAATCTCCCTTGGGTTTAAATCCTAAAACCGATGGGGAGCGATTGATTGCTGCTATTCCTCCATTGACCAAAGAGCATGTTCAGGCTATCTGTAAAGTGGTTAACAAGTCTTGTGAAGATGGCAGACTAAGCATAAGAAGAGGTCGCCAAAAGGCAATGGATACAATTAAAAAGCTATATTCAAGCTACCCTAAGGACAACTTAAAAAGACTGGAGAAAGAGGTTGAGGAGCTGACTAAAAAGCATATCAAGAAAGCAGAGGACTTGTGCAAGGCAAAGGAGAAGGAGATTGCTGGTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRF

Protein Analysis

258

Amino Acids

28.23

Weight (kDa)

9.51

Isoelectric Point (pI)

52.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRF PF01765 96 - 254 2.1e-53 Ribosome recycling factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 85
AccII CGCG 2 cut(s) 21, 41
AciI CCGC 5 cut(s) 21, 83, 104, 275, 386
AclWI GGATC 1 cut(s) 428
AcsI RAATTY 1 cut(s) 134
AcyI GRCGYC 1 cut(s) 101
AfaI GTAC 1 cut(s) 68
AfiI CCNNNNNNNGG 1 cut(s) 224
AgsI TTSAA 6 cut(s) 134, 364, 408, 448, 473, 654
AjnI CCWGG 1 cut(s) 322
AluBI AGCT 8 cut(s) 57, 62, 72, 93, 367, 646, 657, 703
AluI AGCT 8 cut(s) 57, 62, 72, 93, 367, 646, 657, 703
Alw26I GTCTC 2 cut(s) 92, 452
AlwI GGATC 1 cut(s) 428
AoxI GGCC 2 cut(s) 169, 240
ApeKI GCWGC 5 cut(s) 72, 90, 266, 367, 521
ApoI RAATTY 1 cut(s) 134
AspLEI GCGC 1 cut(s) 43
AspS9I GGNCC 2 cut(s) 227, 334
AsuHPI GGTGA 1 cut(s) 312
AvaII GGWCC 2 cut(s) 227, 334
AxyI CCTNAGG 1 cut(s) 663
BbvI GCAGC 5 cut(s) 59, 102, 253, 354, 508
BccI CCATC 3 cut(s) 242, 497, 584
BceAI ACGGC 1 cut(s) 156
BcgI CGANNNNNNTGC 2 cut(s) 142, 176
BciT130I CCWGG 1 cut(s) 324
BciVI GTATCC 1 cut(s) 625
BcoDI GTCTC 2 cut(s) 92, 452
BfmI CTRYAG 1 cut(s) 88
BfuI GTATCC 1 cut(s) 625
BisI GCNGC 7 cut(s) 73, 91, 104, 267, 276, 368, 522
BlpI GCTNAGC 1 cut(s) 58
BlsI GCNGC 7 cut(s) 74, 92, 105, 268, 277, 369, 523
Bme1390I CCNGG 1 cut(s) 324
Bme18I GGWCC 2 cut(s) 227, 334
BmgT120I GGNCC 2 cut(s) 227, 334
BmiI GGNNCC 2 cut(s) 228, 229
BmrFI CCNGG 1 cut(s) 324
BmsI GCATC 2 cut(s) 326, 385
BpmI CTGGAG 1 cut(s) 704
Bpu1102I GCTNAGC 1 cut(s) 58
Bsa29I ATCGAT 1 cut(s) 419
BsaHI GRCGYC 1 cut(s) 101
BsaJI CCNNGG 1 cut(s) 480
BsaWI WCCGGW 1 cut(s) 308
Bsc4I CCNNNNNNNGG 1 cut(s) 224
Bse1I ACTGG 2 cut(s) 354, 687
Bse21I CCTNAGG 1 cut(s) 663
Bse3DI GCAATG 1 cut(s) 633
BseBI CCWGG 1 cut(s) 324
BseCI ATCGAT 1 cut(s) 419
BseDI CCNNGG 1 cut(s) 480
BseLI CCNNNNNNNGG 1 cut(s) 224
BseMI GCAATG 1 cut(s) 633
BseMII CTCAG 2 cut(s) 72, 284
BseNI ACTGG 2 cut(s) 354, 687
BseRI GAGGAG 1 cut(s) 713
BseXI GCAGC 5 cut(s) 59, 102, 253, 354, 508
Bsh1236I CGCG 2 cut(s) 21, 41
BshFI GGCC 2 cut(s) 171, 242
BshVI ATCGAT 1 cut(s) 419
BsiSI CCGG 2 cut(s) 125, 309
BslFI GGGAC 1 cut(s) 240
BslI CCNNNNNNNGG 1 cut(s) 224
BsmAI GTCTC 2 cut(s) 92, 452
BsmBI CGTCTC 1 cut(s) 92
BsmFI GGGAC 1 cut(s) 240
BsmI GAATGC 1 cut(s) 333
BsnI GGCC 2 cut(s) 171, 242
Bsp1407I TGTACA 1 cut(s) 66
Bsp143I GATC 4 cut(s) 6, 76, 420, 433
Bsp1720I GCTNAGC 1 cut(s) 58
BspACI CCGC 5 cut(s) 21, 83, 104, 275, 386
BspANI GGCC 2 cut(s) 171, 242
BspCNI CTCAG 2 cut(s) 71, 285
BspDI ATCGAT 1 cut(s) 419
BspFNI CGCG 2 cut(s) 21, 41
BspLI GGNNCC 2 cut(s) 228, 229
BspMAI CTGCAG 1 cut(s) 92
BspPI GGATC 1 cut(s) 428
BspQI GCTCTTC 1 cut(s) 307
BsrBI CCGCTC 1 cut(s) 85
BsrDI GCAATG 1 cut(s) 633
BsrGI TGTACA 1 cut(s) 66
BsrI ACTGG 2 cut(s) 354, 687
BssECI CCNNGG 1 cut(s) 480
BssMI GATC 4 cut(s) 6, 76, 420, 433
BssNI GRCGYC 1 cut(s) 101
BssT1I CCWWGG 1 cut(s) 480
Bst2UI CCWGG 1 cut(s) 324
Bst6I CTCTTC 3 cut(s) 126, 307, 604
BstACI GRCGYC 1 cut(s) 101
BstAUI TGTACA 1 cut(s) 66
BstDEI CTNAG 4 cut(s) 58, 293, 599, 663
BstFNI CGCG 2 cut(s) 21, 41
BstHHI GCGC 1 cut(s) 43
BstKTI GATC 4 cut(s) 9, 79, 423, 436
BstMAI GTCTC 2 cut(s) 92, 452
BstMBI GATC 4 cut(s) 6, 76, 420, 433
BstMWI GCNNNNNNNGC 5 cut(s) 272, 275, 303, 373, 600
BstNI CCWGG 1 cut(s) 324
BstNSI RCATGY 1 cut(s) 551
BstSCI CCNGG 1 cut(s) 322
BstSFI CTRYAG 1 cut(s) 88
BstUI CGCG 2 cut(s) 21, 41
BstV1I GCAGC 5 cut(s) 59, 102, 253, 354, 508
Bsu15I ATCGAT 1 cut(s) 419
Bsu36I CCTNAGG 1 cut(s) 663
BsuI GTATCC 1 cut(s) 625
BsuRI GGCC 2 cut(s) 171, 242
BsuTUI ATCGAT 1 cut(s) 419
BtsIMutI CAGTG 1 cut(s) 361
CfoI GCGC 1 cut(s) 43
Cfr13I GGNCC 2 cut(s) 227, 334
ClaI ATCGAT 1 cut(s) 419
CseI GACGC 2 cut(s) 27, 109
Csp6I GTAC 1 cut(s) 67
CviAII CATG 2 cut(s) 400, 548
CviQI GTAC 1 cut(s) 67
DdeI CTNAG 4 cut(s) 58, 293, 599, 663
DpnI GATC 4 cut(s) 8, 78, 422, 435
DpnII GATC 4 cut(s) 6, 76, 420, 433
DraI TTTAAA 1 cut(s) 489
Eam1104I CTCTTC 3 cut(s) 126, 307, 604
EarI CTCTTC 3 cut(s) 126, 307, 604
Eco130I CCWWGG 1 cut(s) 480
Eco47I GGWCC 2 cut(s) 227, 334
Eco81I CCTNAGG 1 cut(s) 663
EcoO109I RGGNCCY 1 cut(s) 227
EcoRII CCWGG 1 cut(s) 322
EcoT14I CCWWGG 1 cut(s) 480
ErhI CCWWGG 1 cut(s) 480
Esp3I CGTCTC 1 cut(s) 92
FaeI CATG 2 cut(s) 403, 551
FaiI YATR 9 cut(s) 224, 258, 260, 401, 455, 549, 605, 649, 717
FalI AAGNNNNNCTT 4 cut(s) 356, 388, 656, 688
FaqI GGGAC 1 cut(s) 240
FatI CATG 2 cut(s) 399, 547
FauI CCCGC 1 cut(s) 90
FauNDI CATATG 1 cut(s) 258
Fnu4HI GCNGC 7 cut(s) 73, 91, 104, 267, 276, 368, 522
Fsp4HI GCNGC 7 cut(s) 73, 91, 104, 267, 276, 368, 522
GlaI GCGC 1 cut(s) 42
GluI GCNGC 7 cut(s) 73, 91, 104, 267, 276, 368, 522
GsuI CTGGAG 1 cut(s) 704
HaeIII GGCC 2 cut(s) 171, 242
HapII CCGG 2 cut(s) 125, 309
HgaI GACGC 2 cut(s) 27, 109
HhaI GCGC 1 cut(s) 43
Hin1I GRCGYC 1 cut(s) 101
Hin1II CATG 2 cut(s) 403, 551
Hin6I GCGC 1 cut(s) 41
HinP1I GCGC 1 cut(s) 41
HincII GTYRAC 1 cut(s) 574
HindII GTYRAC 1 cut(s) 574
HinfI GANTC 5 cut(s) 24, 112, 188, 404, 473
HpaI GTTAAC 1 cut(s) 574
HpaII CCGG 2 cut(s) 125, 309
HphI GGTGA 1 cut(s) 312
Hpy166II GTNNAC 1 cut(s) 574
Hpy188I TCNGA 5 cut(s) 17, 35, 50, 97, 381
Hpy188III TCNNGA 3 cut(s) 131, 195, 721
Hpy8I GTNNAC 1 cut(s) 574
Hpy99I CGWCG 2 cut(s) 21, 54
HpyAV CCTTC 1 cut(s) 748
HpyCH4V TGCA 4 cut(s) 90, 269, 376, 741
HpyF10VI GCNNNNNNNGC 5 cut(s) 272, 275, 303, 373, 600
HpyF3I CTNAG 4 cut(s) 58, 293, 599, 663
Hsp92I GRCGYC 1 cut(s) 101
Hsp92II CATG 2 cut(s) 403, 551
HspAI GCGC 1 cut(s) 41
KflI GGGWCCC 1 cut(s) 227
KspAI GTTAAC 1 cut(s) 574
Kzo9I GATC 4 cut(s) 6, 76, 420, 433
LguI GCTCTTC 1 cut(s) 307
LmnI GCTCC 3 cut(s) 98, 508, 700
Lsp1109I GCAGC 5 cut(s) 59, 102, 253, 354, 508
LweI GCATC 2 cut(s) 326, 385
MalI GATC 4 cut(s) 8, 78, 422, 435
MbiI CCGCTC 1 cut(s) 85
MboI GATC 4 cut(s) 6, 76, 420, 433
MboII GAAGA 5 cut(s) 113, 183, 324, 599, 621
MfeI CAATTG 1 cut(s) 270
MluCI AATT 3 cut(s) 134, 270, 636
MmeI TCCRAC 2 cut(s) 40, 73
MseI TTAA 5 cut(s) 488, 573, 639, 674, 772
MslI CAYNNNNRTG 1 cut(s) 344
MspA1I CMGCKG 1 cut(s) 72
MspI CCGG 2 cut(s) 125, 309
MspR9I CCNGG 1 cut(s) 324
MunI CAATTG 1 cut(s) 270
Mva1269I GAATGC 1 cut(s) 333
MvaI CCWGG 1 cut(s) 324
MvnI CGCG 2 cut(s) 21, 41
MwoI GCNNNNNNNGC 5 cut(s) 272, 275, 303, 373, 600
NdeI CATATG 1 cut(s) 258
NdeII GATC 4 cut(s) 6, 76, 420, 433
NlaIII CATG 2 cut(s) 403, 551
NlaIV GGNNCC 2 cut(s) 228, 229
NspI RCATGY 1 cut(s) 551
PciSI GCTCTTC 1 cut(s) 307
PcsI WCGNNNNNNNCGW 1 cut(s) 425
PctI GAATGC 1 cut(s) 333
PfeI GAWTC 5 cut(s) 24, 112, 188, 404, 473
PkrI GCNGC 7 cut(s) 74, 92, 105, 268, 277, 369, 523
PpuMI RGGWCCY 1 cut(s) 227
Psp5II RGGWCCY 1 cut(s) 227
Psp6I CCWGG 1 cut(s) 322
PspGI CCWGG 1 cut(s) 322
PspN4I GGNNCC 2 cut(s) 228, 229
PspPI GGNCC 2 cut(s) 227, 334
PspPPI RGGWCCY 1 cut(s) 227
PstI CTGCAG 1 cut(s) 92
PvuII CAGCTG 1 cut(s) 72
RsaI GTAC 1 cut(s) 68
RsaNI GTAC 1 cut(s) 67
RseI CAYNNNNRTG 1 cut(s) 344
SapI GCTCTTC 1 cut(s) 307
SaqAI TTAA 5 cut(s) 488, 573, 639, 674, 772
SatI GCNGC 7 cut(s) 73, 91, 104, 267, 276, 368, 522
Sau3AI GATC 4 cut(s) 6, 76, 420, 433
Sau96I GGNCC 2 cut(s) 227, 334
ScrFI CCNGG 1 cut(s) 324
SfaNI GCATC 2 cut(s) 326, 385
SfcI CTRYAG 1 cut(s) 88
SinI GGWCC 2 cut(s) 227, 334
SmiMI CAYNNNNRTG 1 cut(s) 344
Sse9I AATT 3 cut(s) 134, 270, 636
SsiI CCGC 5 cut(s) 21, 83, 104, 275, 386
StyD4I CCNGG 1 cut(s) 322
StyI CCWWGG 1 cut(s) 480
TaqI TCGA 1 cut(s) 419
TasI AATT 3 cut(s) 134, 270, 636
TatI WGTACW 1 cut(s) 66
TauI GCSGC 2 cut(s) 106, 278
TfiI GAWTC 5 cut(s) 24, 112, 188, 404, 473
Tru1I TTAA 5 cut(s) 488, 573, 639, 674, 772
Tru9I TTAA 5 cut(s) 488, 573, 639, 674, 772
TscAI CASTG 1 cut(s) 361
TseI GCWGC 5 cut(s) 72, 90, 266, 367, 521
TspDTI ATGAA 1 cut(s) 201
TspGWI ACGGA 1 cut(s) 417
TspRI CASTG 1 cut(s) 361
VpaK11BI GGWCC 2 cut(s) 227, 334
XapI RAATTY 1 cut(s) 134
XceI RCATGY 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.