Rorug02G0579800

Ribosome-recycling factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007948
Physical Location & Seq
Reverse (-)
70295560 .. 70316807
21248 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0579800.1

Sequence Viewer

Length: 1353 bp
ATGTCAGACCTCCCCAACCCTCTCTACGATTTCATCATTCTCGGCGCCTCCGGCTTCACCGGAAAGTACGTCGTGAAAGAAGCCCTCAAGTTCCTCAACACCCCGTCTTCACCTCTCAAGTCTCTGGCTTTAGCCGGCCGCAACCCCGCAAAGCTCTCCCAAACTCTCAACTGGGCGACCGGCCCAAACCCACCTCCCTCCATTCCCATCCTCACCGCCGACACCACCGACCCTCCGTCCCTCCGCCGCCTCTGCTCCCAAACCCGCCTCATCCTCAACTGCGTGGGCCCCTTCCGCCTCTACGGCGAGCCCGTCGTCGCCGCGTGCGTTGAGACCGGCTGTGATTATCTCGACATATGCGGAGAGCCGGAGTTCATGGAGAGGATGGAGGCCAGTTATTTTGATAAGGCGGCGGAGAAGGGCTCGTTGGTCATTTCGGCTTGTGGGTTCGACTCTGTTCCGGCGGAGTTTGGGTTGATGTTTAACTCGAGGCAGTGGGTGGGGGCGGCGGTGCCGAATAGGGTGGAGGCTTATGTGAGCTTGGAGTCTGAGAAGAGACTTGTTTTGAACTTCGGGACGTTTGAGTCGGCGGTGTTGGGTGTGGCTAATGCGGATAAGTTGGAGCTCCGGCGGTCCAGACCTAAAAAGCCGAGGTCGGAGATTCCTGGTCCTCCTCCTTCTAAAGGACCAACTATTGAACACCAGAAAAAGATTGGCCTTTGGGCTGTGAAGCTACCTTCAGCAGATACTATCGTTGTACGAAGAACACTTGGAGCTCTGACTGAAAATCCCGGTGGTCTTCCAGGGAGGAATGAGAGTGATGAACACATTAAAAAAAGAGAAGCCTTCTGGTCAACAGTGAAGCCAGCTTATTTCGGAGTGAAGATAGGCACCAAGTCTCTGCTGGGCATTTTTCGGATGATAATAATTGGAATCTTTCTTGGGCTCTTGGGTCAGTTTTCCTTTGGGAGGTGGCTTCTCTTAAAGTATCCCTCATTTTTCAGTGTTGGATGGTTCAGGAAGCAGGGTCCCTCCGATGAGGAGGTGAGAAGTGCATCTTTTAAGATGTGGTTTGTTGGACGTGGTTTCAGCGACAGGAGTCTTGCTTCTCAGGGAAACACAAAACCTGATATGGAAATAGTAACAAGAGTTATGGGACCTGAAGTTGGCTACATAACAACCCCGATAATTCTACTTCAGTGTGCACTTACTGTTCTGAGCCAACGCGACAATCTTCCAAAGGGAGGAGTTCTCCCACCTGGGATTGTATTTGGCCCAACTGATCTCCAAGAAAAGCTACAAGAAAATGGAATTACTTTTGATGTTATTTCAAAGAATGTTATTTCTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRF

Protein Analysis

450

Amino Acids

49.28

Weight (kDa)

9.1

Isoelectric Point (pI)

44.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sacchrp_dh_NADP PF03435 12 - 144 1.2e-11 Saccharopine dehydrogenase NADP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 583
AccB1I GGYRCC 3 cut(s) 44, 511, 890
AccII CGCG 2 cut(s) 323, 1227
AcoI YGGCCR 1 cut(s) 136
AcuI CTGAAG 3 cut(s) 723, 1181, 1182
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 2 cut(s) 68, 759
AfiI CCNNNNNNNGG 4 cut(s) 683, 969, 1166, 1244
AgsI TTSAA 3 cut(s) 568, 698, 1332
AhdI GACNNNNNGTC 1 cut(s) 235
AjiI CACGTC 1 cut(s) 1082
AjnI CCWGG 3 cut(s) 664, 802, 1258
AjuI GAANNNNNNNTTGG 2 cut(s) 410, 442
AluBI AGCT 7 cut(s) 154, 540, 625, 733, 776, 869, 1297
AluI AGCT 7 cut(s) 154, 540, 625, 733, 776, 869, 1297
Alw21I GWGCWC 3 cut(s) 627, 778, 1207
Alw26I GTCTC 4 cut(s) 126, 326, 550, 903
Alw44I GTGCAC 1 cut(s) 1203
Ama87I CYCGRG 1 cut(s) 487
AoxI GGCC 6 cut(s) 136, 181, 286, 390, 715, 1273
ApaI GGGCCC 1 cut(s) 290
ApaLI GTGCAC 1 cut(s) 1203
AspLEI GCGC 1 cut(s) 47
AspS9I GGNCC 9 cut(s) 182, 286, 287, 633, 668, 686, 1028, 1157, 1274
AsuC2I CCSGG 1 cut(s) 792
AsuHPI GGTGA 4 cut(s) 49, 102, 205, 1057
AvaI CYCGRG 1 cut(s) 487
AvaII GGWCC 5 cut(s) 633, 668, 686, 1028, 1157
BaeGI GKGCMC 2 cut(s) 290, 1207
BaeI ACNNNNGTAYC 2 cut(s) 738, 771
BanI GGYRCC 3 cut(s) 44, 511, 890
BanII GRGCYC 6 cut(s) 290, 312, 425, 627, 778, 948
BbsI GAAGAC 2 cut(s) 99, 791
Bbv12I GWGCWC 3 cut(s) 627, 778, 1207
BccI CCATC 3 cut(s) 215, 379, 1005
BceAI ACGGC 1 cut(s) 319
BciT130I CCWGG 3 cut(s) 666, 804, 1260
BciVI GTATCC 1 cut(s) 999
BcnI CCSGG 1 cut(s) 792
BcoDI GTCTC 4 cut(s) 126, 326, 550, 903
BfoI RGCGCY 1 cut(s) 48
BfuI GTATCC 1 cut(s) 999
BglI GCCNNNNNGGC 1 cut(s) 303
BisI GCNGC 5 cut(s) 139, 247, 321, 411, 507
BlsI GCNGC 5 cut(s) 140, 248, 322, 412, 508
Bme1390I CCNGG 4 cut(s) 666, 792, 804, 1260
Bme18I GGWCC 5 cut(s) 633, 668, 686, 1028, 1157
BmeRI GACNNNNNGTC 1 cut(s) 235
BmeT110I CYCGRG 1 cut(s) 487
BmgBI CACGTC 1 cut(s) 1082
BmgT120I GGNCC 9 cut(s) 182, 286, 287, 633, 668, 686, 1028, 1157, 1274
BmiI GGNNCC 8 cut(s) 46, 288, 289, 513, 892, 1029, 1030, 1158
BmrFI CCNGG 4 cut(s) 666, 792, 804, 1260
BmrI ACTGGG 1 cut(s) 181
BmsI GCATC 1 cut(s) 1064
BmuI ACTGGG 1 cut(s) 181
BoxI GACNNNNGTC 1 cut(s) 1098
BpiI GAAGAC 2 cut(s) 99, 791
BplI GAGNNNNNCTC 4 cut(s) 407, 439, 1236, 1268
BpuEI CTTGAG 2 cut(s) 71, 101
BpuMI CCSGG 1 cut(s) 792
BsaHI GRCGYC 1 cut(s) 45
BsaI GGTCTC 1 cut(s) 326
BsaJI CCNNGG 3 cut(s) 650, 803, 1259
BsaWI WCCGGW 1 cut(s) 59
BsaXI ACNNNNNCTCC 4 cut(s) 217, 247, 458, 488
Bsc4I CCNNNNNNNGG 4 cut(s) 683, 969, 1166, 1244
Bse118I RCCGGY 3 cut(s) 134, 179, 335
Bse1I ACTGG 2 cut(s) 176, 393
BseBI CCWGG 3 cut(s) 666, 804, 1260
BseDI CCNNGG 3 cut(s) 650, 803, 1259
BseGI GGATG 5 cut(s) 207, 270, 390, 924, 1016
BseLI CCNNNNNNNGG 4 cut(s) 683, 969, 1166, 1244
BseMII CTCAG 3 cut(s) 540, 1124, 1208
BseNI ACTGG 2 cut(s) 176, 393
BseRI GAGGAG 3 cut(s) 663, 1055, 1260
BseSI GKGCMC 2 cut(s) 290, 1207
BseX3I CGGCCG 1 cut(s) 136
BseYI CCCAGC 1 cut(s) 904
Bsh1236I CGCG 2 cut(s) 323, 1227
Bsh1285I CGRYCG 2 cut(s) 139, 180
BshFI GGCC 6 cut(s) 138, 183, 288, 392, 717, 1275
BshNI GGYRCC 3 cut(s) 44, 511, 890
BsiEI CGRYCG 2 cut(s) 139, 180
BsiHKAI GWGCWC 3 cut(s) 627, 778, 1207
BsiHKCI CYCGRG 1 cut(s) 487
BsiSI CCGG 9 cut(s) 51, 60, 135, 180, 336, 368, 461, 628, 792
BslFI GGGAC 4 cut(s) 223, 589, 1014, 1170
BslI CCNNNNNNNGG 4 cut(s) 683, 969, 1166, 1244
BsmAI GTCTC 4 cut(s) 126, 326, 550, 903
BsmFI GGGAC 4 cut(s) 223, 589, 1014, 1170
BsnI GGCC 6 cut(s) 138, 183, 288, 392, 717, 1275
Bso31I GGTCTC 1 cut(s) 326
BsoBI CYCGRG 1 cut(s) 487
Bsp120I GGGCCC 1 cut(s) 286
Bsp1286I GDGCHC 7 cut(s) 290, 312, 425, 627, 778, 948, 1207
Bsp143I GATC 1 cut(s) 1282
BspANI GGCC 6 cut(s) 138, 183, 288, 392, 717, 1275
BspCNI CTCAG 3 cut(s) 541, 1123, 1209
BspFNI CGCG 2 cut(s) 323, 1227
BspLI GGNNCC 8 cut(s) 46, 288, 289, 513, 892, 1029, 1030, 1158
BspT107I GGYRCC 3 cut(s) 44, 511, 890
BspTNI GGTCTC 1 cut(s) 326
BsrFI RCCGGY 3 cut(s) 134, 179, 335
BsrI ACTGG 2 cut(s) 176, 393
BssAI RCCGGY 3 cut(s) 134, 179, 335
BssECI CCNNGG 3 cut(s) 650, 803, 1259
BssMI GATC 1 cut(s) 1282
BssNI GRCGYC 1 cut(s) 45
Bst2UI CCWGG 3 cut(s) 666, 804, 1260
Bst4CI ACNGT 2 cut(s) 859, 1213
Bst6I CTCTTC 1 cut(s) 548
BstACI GRCGYC 1 cut(s) 45
BstC8I GCNNGC 4 cut(s) 136, 308, 325, 867
BstDEI CTNAG 3 cut(s) 549, 1110, 1217
BstENI CCTNNNNNAGG 2 cut(s) 681, 967
BstF5I GGATG 5 cut(s) 207, 270, 390, 924, 1016
BstFNI CGCG 2 cut(s) 323, 1227
BstH2I RGCGCY 1 cut(s) 48
BstHHI GCGC 1 cut(s) 47
BstKTI GATC 1 cut(s) 1285
BstMAI GTCTC 4 cut(s) 126, 326, 550, 903
BstMBI GATC 1 cut(s) 1282
BstMCI CGRYCG 2 cut(s) 139, 180
BstMWI GCNNNNNNNGC 4 cut(s) 51, 252, 294, 303
BstNI CCWGG 3 cut(s) 666, 804, 1260
BstPAI GACNNNNGTC 1 cut(s) 1098
BstSCI CCNGG 4 cut(s) 664, 790, 802, 1258
BstSLI GKGCMC 2 cut(s) 290, 1207
BstUI CGCG 2 cut(s) 323, 1227
BstV2I GAAGAC 2 cut(s) 99, 791
BstZI CGGCCG 1 cut(s) 136
BsuI GTATCC 1 cut(s) 999
BsuRI GGCC 6 cut(s) 138, 183, 288, 392, 717, 1275
BtrI CACGTC 1 cut(s) 1082
BtsCI GGATG 5 cut(s) 207, 270, 390, 924, 1016
BtsI GCAGTG 1 cut(s) 500
BtsIMutI CAGTG 4 cut(s) 500, 864, 1009, 1205
Cac8I GCNNGC 4 cut(s) 136, 308, 325, 867
CfoI GCGC 1 cut(s) 47
Cfr10I RCCGGY 3 cut(s) 134, 179, 335
Cfr13I GGNCC 9 cut(s) 182, 286, 287, 633, 668, 686, 1028, 1157, 1274
Csp6I GTAC 2 cut(s) 67, 758
CviAII CATG 1 cut(s) 376
CviQI GTAC 2 cut(s) 67, 758
DdeI CTNAG 3 cut(s) 549, 1110, 1217
DinI GGCGCC 1 cut(s) 46
DpnI GATC 1 cut(s) 1284
DpnII GATC 1 cut(s) 1282
DrdI GACNNNNNNGTC 1 cut(s) 583
DriI GACNNNNNGTC 1 cut(s) 235
DseDI GACNNNNNNGTC 1 cut(s) 583
EaeI YGGCCR 1 cut(s) 136
EagI CGGCCG 1 cut(s) 136
Eam1104I CTCTTC 1 cut(s) 548
Eam1105I GACNNNNNGTC 1 cut(s) 235
EarI CTCTTC 1 cut(s) 548
EciI GGCGGA 4 cut(s) 233, 284, 428, 479
Ecl136II GAGCTC 2 cut(s) 625, 776
EclXI CGGCCG 1 cut(s) 136
Eco24I GRGCYC 6 cut(s) 290, 312, 425, 627, 778, 948
Eco31I GGTCTC 1 cut(s) 326
Eco47I GGWCC 5 cut(s) 633, 668, 686, 1028, 1157
Eco52I CGGCCG 1 cut(s) 136
Eco53kI GAGCTC 2 cut(s) 625, 776
Eco57I CTGAAG 3 cut(s) 723, 1181, 1182
Eco88I CYCGRG 1 cut(s) 487
EcoICRI GAGCTC 2 cut(s) 625, 776
EcoNI CCTNNNNNAGG 2 cut(s) 681, 967
EcoO109I RGGNCCY 3 cut(s) 287, 1028, 1157
EcoRII CCWGG 3 cut(s) 664, 802, 1258
EcoT38I GRGCYC 6 cut(s) 290, 312, 425, 627, 778, 948
EgeI GGCGCC 1 cut(s) 46
EheI GGCGCC 1 cut(s) 46
FaeI CATG 1 cut(s) 379
FaiI YATR 7 cut(s) 356, 358, 377, 534, 1133, 1154, 1175
FalI AAGNNNNNCTT 2 cut(s) 1042, 1074
FaqI GGGAC 4 cut(s) 223, 589, 1014, 1170
FatI CATG 1 cut(s) 375
FauI CCCGC 2 cut(s) 154, 272
FauNDI CATATG 1 cut(s) 356
Fnu4HI GCNGC 5 cut(s) 139, 247, 321, 411, 507
FokI GGATG 5 cut(s) 194, 257, 397, 931, 1023
FriOI GRGCYC 6 cut(s) 290, 312, 425, 627, 778, 948
Fsp4HI GCNGC 5 cut(s) 139, 247, 321, 411, 507
GlaI GCGC 1 cut(s) 46
GluI GCNGC 5 cut(s) 139, 247, 321, 411, 507
GsaI CCCAGC 1 cut(s) 908
HaeII RGCGCY 1 cut(s) 48
HaeIII GGCC 6 cut(s) 138, 183, 288, 392, 717, 1275
HapII CCGG 9 cut(s) 51, 60, 135, 180, 336, 368, 461, 628, 792
HhaI GCGC 1 cut(s) 47
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 1 cut(s) 379
Hin6I GCGC 1 cut(s) 45
HinP1I GCGC 1 cut(s) 45
HincII GTYRAC 1 cut(s) 855
HindII GTYRAC 1 cut(s) 855
HinfI GANTC 6 cut(s) 452, 545, 584, 661, 933, 1099
HpaII CCGG 9 cut(s) 51, 60, 135, 180, 336, 368, 461, 628, 792
HphI GGTGA 4 cut(s) 49, 102, 205, 1057
Hpy166II GTNNAC 2 cut(s) 855, 1205
Hpy188I TCNGA 8 cut(s) 7, 550, 658, 780, 878, 918, 1036, 1218
Hpy188III TCNNGA 5 cut(s) 73, 350, 574, 636, 1018
Hpy8I GTNNAC 2 cut(s) 855, 1205
Hpy99I CGWCG 3 cut(s) 74, 317, 320
HpyAV CCTTC 5 cut(s) 301, 412, 687, 747, 856
HpyCH4III ACNGT 2 cut(s) 859, 1213
HpyCH4IV ACGT 3 cut(s) 69, 578, 1081
HpyCH4V TGCA 2 cut(s) 1055, 1205
HpyF10VI GCNNNNNNNGC 4 cut(s) 51, 252, 294, 303
HpyF3I CTNAG 3 cut(s) 549, 1110, 1217
HpySE526I ACGT 3 cut(s) 69, 578, 1081
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 1 cut(s) 379
HspAI GCGC 1 cut(s) 45
KasI GGCGCC 1 cut(s) 44
KflI GGGWCCC 1 cut(s) 1028
KroI GCCGGC 1 cut(s) 134
KroNI GCCGGC 1 cut(s) 136
Kzo9I GATC 1 cut(s) 1282
LmnI GCTCC 4 cut(s) 260, 622, 630, 773
LweI GCATC 1 cut(s) 1064
MaeII ACGT 3 cut(s) 69, 578, 1081
MaeIII GTNAC 1 cut(s) 1141
MalI GATC 1 cut(s) 1284
MboI GATC 1 cut(s) 1282
MboII GAAGA 6 cut(s) 99, 565, 774, 791, 895, 1226
MhlI GDGCHC 7 cut(s) 290, 312, 425, 627, 778, 948, 1207
MluCI AATT 3 cut(s) 927, 1188, 1311
Mly113I GGCGCC 1 cut(s) 45
MlyI GAGTC 4 cut(s) 446, 554, 593, 1108
MmeI TCCRAC 4 cut(s) 600, 636, 988, 1057
MroNI GCCGGC 1 cut(s) 134
MseI TTAA 5 cut(s) 483, 831, 983, 1062, 1351
MspI CCGG 9 cut(s) 51, 60, 135, 180, 336, 368, 461, 628, 792
MspR9I CCNGG 4 cut(s) 666, 792, 804, 1260
MvaI CCWGG 3 cut(s) 666, 804, 1260
MvnI CGCG 2 cut(s) 323, 1227
MwoI GCNNNNNNNGC 4 cut(s) 51, 252, 294, 303
NaeI GCCGGC 1 cut(s) 136
NarI GGCGCC 1 cut(s) 45
NciI CCSGG 1 cut(s) 792
NdeI CATATG 1 cut(s) 356
NdeII GATC 1 cut(s) 1282
NgoMIV GCCGGC 1 cut(s) 134
NlaIII CATG 1 cut(s) 379
NlaIV GGNNCC 8 cut(s) 46, 288, 289, 513, 892, 1029, 1030, 1158
NmeAIII GCCGAG 2 cut(s) 21, 675
PaeR7I CTCGAG 1 cut(s) 487
PcsI WCGNNNNNNNCGW 2 cut(s) 309, 324
PdiI GCCGGC 1 cut(s) 136
PfeI GAWTC 2 cut(s) 661, 933
PkrI GCNGC 5 cut(s) 140, 248, 322, 412, 508
PleI GAGTC 4 cut(s) 446, 553, 592, 1107
PluTI GGCGCC 1 cut(s) 48
PpsI GAGTC 4 cut(s) 446, 553, 592, 1107
PpuMI RGGWCCY 2 cut(s) 1028, 1157
PshAI GACNNNNGTC 1 cut(s) 1098
Psp124BI GAGCTC 2 cut(s) 627, 778
Psp5II RGGWCCY 2 cut(s) 1028, 1157
Psp6I CCWGG 3 cut(s) 664, 802, 1258
PspFI CCCAGC 1 cut(s) 904
PspGI CCWGG 3 cut(s) 664, 802, 1258
PspN4I GGNNCC 8 cut(s) 46, 288, 289, 513, 892, 1029, 1030, 1158
PspOMI GGGCCC 1 cut(s) 286
PspPI GGNCC 9 cut(s) 182, 286, 287, 633, 668, 686, 1028, 1157, 1274
PspPPI RGGWCCY 2 cut(s) 1028, 1157
PspXI VCTCGAGB 1 cut(s) 487
RsaI GTAC 2 cut(s) 68, 759
RsaNI GTAC 2 cut(s) 67, 758
SacI GAGCTC 2 cut(s) 627, 778
SaqAI TTAA 5 cut(s) 483, 831, 983, 1062, 1351
SatI GCNGC 5 cut(s) 139, 247, 321, 411, 507
Sau3AI GATC 1 cut(s) 1282
Sau96I GGNCC 9 cut(s) 182, 286, 287, 633, 668, 686, 1028, 1157, 1274
SchI GAGTC 4 cut(s) 446, 554, 593, 1108
ScrFI CCNGG 4 cut(s) 666, 792, 804, 1260
SduI GDGCHC 7 cut(s) 290, 312, 425, 627, 778, 948, 1207
SfaNI GCATC 1 cut(s) 1064
SfoI GGCGCC 1 cut(s) 46
Sfr274I CTCGAG 1 cut(s) 487
SinI GGWCC 5 cut(s) 633, 668, 686, 1028, 1157
SlaI CTCGAG 1 cut(s) 487
SmlI CTYRAG 3 cut(s) 86, 116, 487
SmoI CTYRAG 3 cut(s) 86, 116, 487
Sse9I AATT 3 cut(s) 927, 1188, 1311
SspDI GGCGCC 1 cut(s) 44
SstI GAGCTC 2 cut(s) 627, 778
StyD4I CCNGG 4 cut(s) 664, 790, 802, 1258
TaaI ACNGT 2 cut(s) 859, 1213
TaiI ACGT 3 cut(s) 72, 581, 1084
TaqI TCGA 3 cut(s) 351, 450, 488
TasI AATT 3 cut(s) 927, 1188, 1311
TauI GCSGC 5 cut(s) 141, 249, 323, 413, 509
TfiI GAWTC 2 cut(s) 661, 933
Tru1I TTAA 5 cut(s) 483, 831, 983, 1062, 1351
Tru9I TTAA 5 cut(s) 483, 831, 983, 1062, 1351
TscAI CASTG 4 cut(s) 500, 864, 1009, 1205
TspDTI ATGAA 3 cut(s) 22, 364, 837
TspGWI ACGGA 1 cut(s) 225
TspRI CASTG 4 cut(s) 500, 864, 1009, 1205
VneI GTGCAC 1 cut(s) 1203
VpaK11BI GGWCC 5 cut(s) 633, 668, 686, 1028, 1157
XagI CCTNNNNNAGG 2 cut(s) 681, 967
XcmI CCANNNNNNNNNTGG 2 cut(s) 710, 901
XhoI CTCGAG 1 cut(s) 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.