Rorug02G0579900

Ribosome-recycling factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007948
Physical Location & Seq
Forward (+)
70337078 .. 70338048
971 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0579900.1

Sequence Viewer

Length: 786 bp
ATGACGAACATGATTGCTGGTGGTGCAGCTGCCCGTCCGTTTGTCACTTATCATAATGATCTTAACATGAAGCTTTTCATGCGGATTGCACCCGAACTCTATCCCAAGCAGCTAGTTGTTGGTGGACTGGACCGTGTATATGAGATTGGAAAGCAATTCAGAAATGAGGGTATTGATTTGACACATAATCCTGAGTTCACCACATGTGAACTTTACATGGCCTATGCTGATTACAATGACTTGATGACGATGCATGACCGAGGAAATGTTGAGTGGGATGGTCAAGGAAATTACCGGAGGAAGTTTGATATCAAATGTTCTCCTCCTGAAACAACAGCTCGTTTGTTGGACAAGCTTGTGGGCCACTTTTTAGAGGAGACGTGTGTAAATCCAGCTTTCATAATCAACCATCCTGAGATAATGAGTCCGTTAGCCAAGTGGCATAGATCAAAGCCTGGCCTGACTGAACGGTTCGAGTTGTTTATCAATAAACATGAAGTTTGCAATGCATACACTGAATTGAATGATCCTGTAGTACAACGCCAACGGTTTGCTGGTCAGCTCAAGGATCGACAGTCGGGTGATGATGAAGCAATGGCTCTGGATGAAACATTTTGTACTGCTCTCGAGTATGGGTTACCTCCTACTGGTGGCTGGGGTTTGGGTGTTGACCGCCTATGTATGTTGTTAACTGACTCACAGAATATTAAAGAAGTGCTTCTCTTCCCAGCCATGAAACCACAAGATGATCAACCTCCTGCAAAACCAACAACTGCAGGTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRF

Protein Analysis

261

Amino Acids

29.56

Weight (kDa)

5.3

Isoelectric Point (pI)

47.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_2 PF00152 5 - 85 6e-26 tRNA synthetases class II (D, K and N)
tRNA-synt_2 PF00152 124 - 245 3.2e-35 tRNA synthetases class II (D, K and N)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 767
Acc36I ACCTGC 1 cut(s) 767
AciI CCGC 2 cut(s) 82, 673
AclWI GGATC 2 cut(s) 521, 576
AfaI GTAC 2 cut(s) 537, 619
AfiI CCNNNNNNNGG 2 cut(s) 647, 650
AflIII ACRYGT 2 cut(s) 203, 380
AgsI TTSAA 1 cut(s) 523
AjiI CACGTC 1 cut(s) 381
AjnI CCWGG 1 cut(s) 454
AluBI AGCT 7 cut(s) 29, 73, 112, 338, 355, 395, 562
AluI AGCT 7 cut(s) 29, 73, 112, 338, 355, 395, 562
Alw26I GTCTC 1 cut(s) 371
AlwI GGATC 2 cut(s) 521, 576
Ama87I CYCGRG 1 cut(s) 626
AoxI GGCC 3 cut(s) 219, 361, 457
ApeKI GCWGC 3 cut(s) 26, 29, 109
Asp700I GAANNNNTTC 2 cut(s) 74, 717
AspS9I GGNCC 2 cut(s) 130, 361
AsuHPI GGTGA 2 cut(s) 190, 593
AvaI CYCGRG 1 cut(s) 626
AvaII GGWCC 1 cut(s) 130
BbvI GCAGC 3 cut(s) 16, 38, 121
BccI CCATC 2 cut(s) 272, 417
BciT130I CCWGG 1 cut(s) 456
BclI TGATCA 1 cut(s) 748
BcoDI GTCTC 1 cut(s) 371
BfaI CTAG 1 cut(s) 113
BfmI CTRYAG 2 cut(s) 531, 774
BfuAI ACCTGC 1 cut(s) 767
BisI GCNGC 3 cut(s) 27, 30, 110
BlsI GCNGC 3 cut(s) 28, 31, 111
Bme1390I CCNGG 1 cut(s) 456
Bme18I GGWCC 1 cut(s) 130
BmeT110I CYCGRG 1 cut(s) 626
BmgBI CACGTC 1 cut(s) 381
BmgT120I GGNCC 2 cut(s) 130, 361
BmrFI CCNGG 1 cut(s) 456
BmsI GCATC 1 cut(s) 240
BpuEI CTTGAG 1 cut(s) 548
BsaJI CCNNGG 1 cut(s) 259
BsaWI WCCGGW 1 cut(s) 294
Bsc4I CCNNNNNNNGG 2 cut(s) 647, 650
Bse1I ACTGG 2 cut(s) 132, 652
Bse3DI GCAATG 2 cut(s) 511, 600
BseBI CCWGG 1 cut(s) 456
BseDI CCNNGG 1 cut(s) 259
BseGI GGATG 3 cut(s) 283, 409, 610
BseLI CCNNNNNNNGG 2 cut(s) 647, 650
BseMI GCAATG 2 cut(s) 511, 600
BseMII CTCAG 2 cut(s) 183, 405
BseNI ACTGG 2 cut(s) 132, 652
BseRI GAGGAG 2 cut(s) 312, 389
BseXI GCAGC 3 cut(s) 16, 38, 121
BseYI CCCAGC 2 cut(s) 654, 727
BsgI GTGCAG 1 cut(s) 45
BshFI GGCC 3 cut(s) 221, 363, 459
BsiHKCI CYCGRG 1 cut(s) 626
BsiSI CCGG 1 cut(s) 295
BslI CCNNNNNNNGG 2 cut(s) 647, 650
BsmAI GTCTC 1 cut(s) 371
BsmBI CGTCTC 1 cut(s) 371
BsnI GGCC 3 cut(s) 221, 363, 459
BsoBI CYCGRG 1 cut(s) 626
Bsp143I GATC 5 cut(s) 58, 446, 526, 568, 748
BspACI CCGC 2 cut(s) 82, 673
BspANI GGCC 3 cut(s) 221, 363, 459
BspCNI CTCAG 2 cut(s) 184, 406
BspMAI CTGCAG 1 cut(s) 778
BspMI ACCTGC 1 cut(s) 767
BspPI GGATC 2 cut(s) 521, 576
BsrDI GCAATG 2 cut(s) 511, 600
BsrI ACTGG 2 cut(s) 132, 652
BssECI CCNNGG 1 cut(s) 259
BssMI GATC 5 cut(s) 58, 446, 526, 568, 748
Bst2UI CCWGG 1 cut(s) 456
Bst4CI ACNGT 4 cut(s) 134, 471, 549, 576
Bst6I CTCTTC 1 cut(s) 728
BstDEI CTNAG 3 cut(s) 192, 414, 783
BstEII GGTNACC 1 cut(s) 636
BstF5I GGATG 3 cut(s) 283, 409, 610
BstKTI GATC 5 cut(s) 61, 449, 529, 571, 751
BstMAI GTCTC 1 cut(s) 371
BstMBI GATC 5 cut(s) 58, 446, 526, 568, 748
BstMWI GCNNNNNNNGC 2 cut(s) 23, 79
BstNI CCWGG 1 cut(s) 456
BstNSI RCATGY 1 cut(s) 207
BstPI GGTNACC 1 cut(s) 636
BstSCI CCNGG 1 cut(s) 454
BstSFI CTRYAG 2 cut(s) 531, 774
BstV1I GCAGC 3 cut(s) 16, 38, 121
BsuRI GGCC 3 cut(s) 221, 363, 459
BtrI CACGTC 1 cut(s) 381
BtsCI GGATG 3 cut(s) 283, 409, 610
BtsIMutI CAGTG 1 cut(s) 513
BveI ACCTGC 1 cut(s) 767
Cfr13I GGNCC 2 cut(s) 130, 361
Csp6I GTAC 2 cut(s) 536, 618
CviAII CATG 8 cut(s) 10, 67, 79, 204, 217, 254, 494, 733
CviQI GTAC 2 cut(s) 536, 618
DdeI CTNAG 3 cut(s) 192, 414, 783
DpnI GATC 5 cut(s) 60, 448, 528, 570, 750
DpnII GATC 5 cut(s) 58, 446, 526, 568, 748
Eam1104I CTCTTC 1 cut(s) 728
EarI CTCTTC 1 cut(s) 728
Eco32I GATATC 1 cut(s) 310
Eco47I GGWCC 1 cut(s) 130
Eco88I CYCGRG 1 cut(s) 626
Eco91I GGTNACC 1 cut(s) 636
EcoO65I GGTNACC 1 cut(s) 636
EcoRII CCWGG 1 cut(s) 454
EcoRV GATATC 1 cut(s) 310
EcoT22I ATGCAT 2 cut(s) 255, 511
Esp3I CGTCTC 1 cut(s) 371
FaeI CATG 8 cut(s) 13, 70, 82, 207, 220, 257, 497, 736
FalI AAGNNNNNCTT 2 cut(s) 702, 734
FatI CATG 8 cut(s) 9, 66, 78, 203, 216, 253, 493, 732
FbaI TGATCA 1 cut(s) 748
Fnu4HI GCNGC 3 cut(s) 27, 30, 110
FokI GGATG 3 cut(s) 290, 396, 617
Fsp4HI GCNGC 3 cut(s) 27, 30, 110
FspBI CTAG 1 cut(s) 113
GluI GCNGC 3 cut(s) 27, 30, 110
GsaI CCCAGC 2 cut(s) 658, 731
HaeIII GGCC 3 cut(s) 221, 363, 459
HapII CCGG 1 cut(s) 295
Hin1II CATG 8 cut(s) 13, 70, 82, 207, 220, 257, 497, 736
HincII GTYRAC 2 cut(s) 670, 690
HindII GTYRAC 2 cut(s) 670, 690
HindIII AAGCTT 2 cut(s) 71, 353
HinfI GANTC 2 cut(s) 424, 695
HpaI GTTAAC 1 cut(s) 690
HpaII CCGG 1 cut(s) 295
HphI GGTGA 2 cut(s) 190, 593
Hpy166II GTNNAC 5 cut(s) 125, 198, 209, 670, 690
Hpy188I TCNGA 1 cut(s) 161
Hpy188III TCNNGA 5 cut(s) 191, 326, 413, 602, 626
Hpy8I GTNNAC 5 cut(s) 125, 198, 209, 670, 690
HpyCH4III ACNGT 4 cut(s) 134, 471, 549, 576
HpyCH4IV ACGT 1 cut(s) 380
HpyCH4V TGCA 7 cut(s) 26, 89, 253, 504, 509, 761, 776
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 79
HpyF3I CTNAG 3 cut(s) 192, 414, 783
HpySE526I ACGT 1 cut(s) 380
Hsp92II CATG 8 cut(s) 13, 70, 82, 207, 220, 257, 497, 736
Ksp22I TGATCA 1 cut(s) 748
KspAI GTTAAC 1 cut(s) 690
Kzo9I GATC 5 cut(s) 58, 446, 526, 568, 748
Lsp1109I GCAGC 3 cut(s) 16, 38, 121
LweI GCATC 1 cut(s) 240
MaeI CTAG 1 cut(s) 113
MaeII ACGT 1 cut(s) 380
MaeIII GTNAC 2 cut(s) 43, 636
MalI GATC 5 cut(s) 60, 448, 528, 570, 750
MboI GATC 5 cut(s) 58, 446, 526, 568, 748
MboII GAAGA 1 cut(s) 715
MluCI AATT 3 cut(s) 155, 289, 518
MlyI GAGTC 2 cut(s) 433, 689
MmeI TCCRAC 1 cut(s) 327
MnlI CCTC 7 cut(s) 160, 254, 291, 333, 367, 651, 765
Mph1103I ATGCAT 2 cut(s) 255, 511
MroXI GAANNNNTTC 2 cut(s) 74, 717
MseI TTAA 3 cut(s) 63, 689, 708
MspA1I CMGCKG 1 cut(s) 29
MspI CCGG 1 cut(s) 295
MspR9I CCNGG 1 cut(s) 456
MvaI CCWGG 1 cut(s) 456
MwoI GCNNNNNNNGC 2 cut(s) 23, 79
NdeII GATC 5 cut(s) 58, 446, 526, 568, 748
NlaIII CATG 8 cut(s) 13, 70, 82, 207, 220, 257, 497, 736
NmuCI GTSAC 1 cut(s) 43
NsiI ATGCAT 2 cut(s) 255, 511
NspI RCATGY 1 cut(s) 207
PaeR7I CTCGAG 1 cut(s) 626
PaqCI CACCTGC 1 cut(s) 767
PciI ACATGT 1 cut(s) 203
PdmI GAANNNNTTC 2 cut(s) 74, 717
PkrI GCNGC 3 cut(s) 28, 31, 111
PleI GAGTC 2 cut(s) 432, 689
PpsI GAGTC 2 cut(s) 432, 689
PscI ACATGT 1 cut(s) 203
Psp6I CCWGG 1 cut(s) 454
PspEI GGTNACC 1 cut(s) 636
PspFI CCCAGC 2 cut(s) 654, 727
PspGI CCWGG 1 cut(s) 454
PspPI GGNCC 2 cut(s) 130, 361
PstI CTGCAG 1 cut(s) 778
PvuII CAGCTG 1 cut(s) 29
RsaI GTAC 2 cut(s) 537, 619
RsaNI GTAC 2 cut(s) 536, 618
SaqAI TTAA 3 cut(s) 63, 689, 708
SatI GCNGC 3 cut(s) 27, 30, 110
Sau3AI GATC 5 cut(s) 58, 446, 526, 568, 748
Sau96I GGNCC 2 cut(s) 130, 361
SchI GAGTC 2 cut(s) 433, 689
ScrFI CCNGG 1 cut(s) 456
SfaNI GCATC 1 cut(s) 240
SfcI CTRYAG 2 cut(s) 531, 774
Sfr274I CTCGAG 1 cut(s) 626
SinI GGWCC 1 cut(s) 130
SlaI CTCGAG 1 cut(s) 626
SmlI CTYRAG 2 cut(s) 563, 626
SmoI CTYRAG 2 cut(s) 563, 626
Sse9I AATT 3 cut(s) 155, 289, 518
SsiI CCGC 2 cut(s) 82, 673
SspI AATATT 1 cut(s) 706
SspMI CTAG 1 cut(s) 113
StyD4I CCNGG 1 cut(s) 454
TaaI ACNGT 4 cut(s) 134, 471, 549, 576
TaiI ACGT 1 cut(s) 383
TaqI TCGA 3 cut(s) 474, 571, 627
TaqII GACCGA 1 cut(s) 273
TasI AATT 3 cut(s) 155, 289, 518
TatI WGTACW 2 cut(s) 535, 617
Tru1I TTAA 3 cut(s) 63, 689, 708
Tru9I TTAA 3 cut(s) 63, 689, 708
TscAI CASTG 1 cut(s) 520
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 3 cut(s) 26, 29, 109
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 7 cut(s) 67, 83, 388, 510, 603, 621, 749
TspGWI ACGGA 2 cut(s) 27, 417
TspRI CASTG 1 cut(s) 520
VpaK11BI GGWCC 1 cut(s) 130
XceI RCATGY 1 cut(s) 207
XcmI CCANNNNNNNNNTGG 1 cut(s) 551
XhoI CTCGAG 1 cut(s) 626
XmnI GAANNNNTTC 2 cut(s) 74, 717
XspI CTAG 1 cut(s) 113
Zsp2I ATGCAT 2 cut(s) 255, 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.