FvH4_6g53070

Ribosome-recycling factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007948
Physical Location & Seq
Forward (+)
38970366 .. 38973308
2943 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g53070.t1

Sequence Viewer

Length: 795 bp
ATGGCGATCTTTCTGAGACGCGGATTCTCGCTCCGAAACGCGCTTCTCCGGCGAATCCCGGCCCTCTGCGGCACCGTGGCGCCGCCGCAGAGGATTACTCTCTCTACTCCTCCTCCTGCAGCTCTTGAATTTGTGAGAGAAAGTCGCAGAGGCTTCGCCAAAGGCCGTAACAAGTCAAGTACCAAGTCAAGGATTGTAGAAGATGAAGATTCTGAAAGCACAGTGGAGGTTTTGCCGGATATGGGGCCCACCATCAAGGCCAATGCGACCACTCATATGGAGGCTGCGATTACAGCGTTGTTGGGAGAACTGAGCAAGCTACGGACAGGGAGAGCATCTCCGGGAATGCTGGACCACATTATTGTGGAAGCCAGTGGGTTGAAGCTGCCGTTGAGTCAGATAGCGGTTGTTTCAGTCATGGATTCGAAGACGTTGTCCATCAATCCGTTTGATCCAAATGCCTTGAAGAATATAGAGACTGCCATTATTGAATGTCCCTTGGGTTTAAATCCTAAAACCGATGGGGATCGATTAATTGCAGCTATTCCTCCATTGACCAAAGAGCATGTTCAGGCTATCGGTAAAGTGGTTAACAAATCTTGTGAAGATGGCAGACTAAGCATTAGAAGAGGTCGCCAAAAGGCAATGGATGCAATTAAAAAGTTATATTCAATCTACCCTAAGGATGACTTAAAAAGATTGGAGAAAGAGGTTGAGGAGCTAACTAAAAAGTATATCAAGAAAGCAGAGGACTTGTGCAAAGCAAAGGAGAAGGAGATTGCTGCTGGAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRF

Protein Analysis

265

Amino Acids

28.86

Weight (kDa)

9.51

Isoelectric Point (pI)

51.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRF PF01765 103 - 260 1.1e-51 Ribosome recycling factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 71, 79
AccII CGCG 2 cut(s) 21, 41
AciI CCGC 5 cut(s) 21, 69, 83, 86, 404
AclWI GGATC 2 cut(s) 446, 534
AcsI RAATTY 1 cut(s) 128
AcyI GRCGYC 1 cut(s) 80
AfaI GTAC 1 cut(s) 181
AfiI CCNNNNNNNGG 2 cut(s) 189, 242
AgsI TTSAA 5 cut(s) 128, 382, 466, 491, 672
AluBI AGCT 5 cut(s) 122, 319, 385, 542, 721
AluI AGCT 5 cut(s) 122, 319, 385, 542, 721
Alw26I GTCTC 2 cut(s) 10, 470
AlwI GGATC 2 cut(s) 446, 534
AoxI GGCC 4 cut(s) 60, 163, 245, 258
ApaI GGGCCC 1 cut(s) 249
ApeKI GCWGC 5 cut(s) 119, 284, 385, 539, 782
ApoI RAATTY 1 cut(s) 128
ArsI GACNNNNNNTTYG 2 cut(s) 419, 451
AseI ATTAAT 1 cut(s) 533
AspLEI GCGC 2 cut(s) 43, 82
AspS9I GGNCC 4 cut(s) 61, 245, 246, 352
AsuC2I CCSGG 2 cut(s) 59, 342
AsuII TTCGAA 1 cut(s) 425
AvaII GGWCC 1 cut(s) 352
AxyI CCTNAGG 1 cut(s) 681
BaeGI GKGCMC 1 cut(s) 249
BanI GGYRCC 2 cut(s) 71, 79
BanII GRGCYC 1 cut(s) 249
BbsI GAAGAC 1 cut(s) 434
BbvI GCAGC 5 cut(s) 131, 271, 372, 551, 769
BccI CCATC 4 cut(s) 260, 446, 515, 602
BceAI ACGGC 2 cut(s) 150, 373
BcgI CGANNNNNNTGC 2 cut(s) 136, 170
BcnI CCSGG 2 cut(s) 59, 342
BcoDI GTCTC 2 cut(s) 10, 470
BfmI CTRYAG 1 cut(s) 117
BfoI RGCGCY 1 cut(s) 83
BisI GCNGC 8 cut(s) 70, 83, 86, 120, 285, 386, 540, 783
BlsI GCNGC 8 cut(s) 71, 84, 87, 121, 286, 387, 541, 784
Bme1390I CCNGG 2 cut(s) 59, 342
Bme18I GGWCC 1 cut(s) 352
BmgT120I GGNCC 4 cut(s) 61, 245, 246, 352
BmiI GGNNCC 4 cut(s) 73, 81, 246, 247
BmrFI CCNGG 2 cut(s) 59, 342
BmsI GCATC 2 cut(s) 344, 640
BpiI GAAGAC 1 cut(s) 434
BplI GAGNNNNNCTC 4 cut(s) 82, 114, 322, 354
Bpu14I TTCGAA 1 cut(s) 425
BpuMI CCSGG 2 cut(s) 59, 342
Bsa29I ATCGAT 1 cut(s) 529
BsaBI GATNNNNATC 1 cut(s) 525
BsaHI GRCGYC 1 cut(s) 80
BsaJI CCNNGG 2 cut(s) 75, 498
BsaXI ACNNNNNCTCC 2 cut(s) 97, 127
Bsc4I CCNNNNNNNGG 2 cut(s) 189, 242
Bse1I ACTGG 1 cut(s) 372
Bse21I CCTNAGG 1 cut(s) 681
Bse3DI GCAATG 1 cut(s) 651
Bse8I GATNNNNATC 1 cut(s) 525
BseCI ATCGAT 1 cut(s) 529
BseDI CCNNGG 2 cut(s) 75, 498
BseGI GGATG 2 cut(s) 655, 691
BseJI GATNNNNATC 1 cut(s) 525
BseLI CCNNNNNNNGG 2 cut(s) 189, 242
BseMI GCAATG 1 cut(s) 651
BseMII CTCAG 2 cut(s) 5, 302
BseNI ACTGG 1 cut(s) 372
BseRI GAGGAG 3 cut(s) 99, 102, 731
BseSI GKGCMC 1 cut(s) 249
BseXI GCAGC 5 cut(s) 131, 271, 372, 551, 769
Bsh1236I CGCG 2 cut(s) 21, 41
BshFI GGCC 4 cut(s) 62, 165, 247, 260
BshNI GGYRCC 2 cut(s) 71, 79
BshVI ATCGAT 1 cut(s) 529
BsiSI CCGG 4 cut(s) 49, 59, 236, 341
BslFI GGGAC 1 cut(s) 480
BslI CCNNNNNNNGG 2 cut(s) 189, 242
BsmAI GTCTC 2 cut(s) 10, 470
BsmBI CGTCTC 1 cut(s) 10
BsmFI GGGAC 1 cut(s) 480
BsmI GAATGC 1 cut(s) 351
BsnI GGCC 4 cut(s) 62, 165, 247, 260
Bsp119I TTCGAA 1 cut(s) 425
Bsp120I GGGCCC 1 cut(s) 245
Bsp1286I GDGCHC 1 cut(s) 249
Bsp143I GATC 3 cut(s) 6, 451, 526
BspACI CCGC 5 cut(s) 21, 69, 83, 86, 404
BspANI GGCC 4 cut(s) 62, 165, 247, 260
BspCNI CTCAG 2 cut(s) 6, 303
BspDI ATCGAT 1 cut(s) 529
BspFNI CGCG 2 cut(s) 21, 41
BspLI GGNNCC 4 cut(s) 73, 81, 246, 247
BspMAI CTGCAG 1 cut(s) 121
BspPI GGATC 2 cut(s) 446, 534
BspT104I TTCGAA 1 cut(s) 425
BspT107I GGYRCC 2 cut(s) 71, 79
BsrDI GCAATG 1 cut(s) 651
BsrI ACTGG 1 cut(s) 372
BssECI CCNNGG 2 cut(s) 75, 498
BssMI GATC 3 cut(s) 6, 451, 526
BssNI GRCGYC 1 cut(s) 80
BssT1I CCWWGG 1 cut(s) 498
Bst4CI ACNGT 2 cut(s) 76, 223
Bst6I CTCTTC 1 cut(s) 622
BstACI GRCGYC 1 cut(s) 80
BstAPI GCANNNNNTGC 1 cut(s) 650
BstBI TTCGAA 1 cut(s) 425
BstC8I GCNNGC 1 cut(s) 317
BstDEI CTNAG 4 cut(s) 14, 311, 617, 681
BstDSI CCRYGG 1 cut(s) 75
BstF5I GGATG 2 cut(s) 655, 691
BstFNI CGCG 2 cut(s) 21, 41
BstH2I RGCGCY 1 cut(s) 83
BstHHI GCGC 2 cut(s) 43, 82
BstKTI GATC 3 cut(s) 9, 454, 529
BstMAI GTCTC 2 cut(s) 10, 470
BstMBI GATC 3 cut(s) 6, 451, 526
BstMWI GCNNNNNNNGC 4 cut(s) 49, 293, 618, 650
BstNSI RCATGY 1 cut(s) 569
BstSCI CCNGG 2 cut(s) 57, 340
BstSFI CTRYAG 1 cut(s) 117
BstSLI GKGCMC 1 cut(s) 249
BstUI CGCG 2 cut(s) 21, 41
BstV1I GCAGC 5 cut(s) 131, 271, 372, 551, 769
BstV2I GAAGAC 1 cut(s) 434
BstXI CCANNNNNNTGG 1 cut(s) 277
Bsu15I ATCGAT 1 cut(s) 529
Bsu36I CCTNAGG 1 cut(s) 681
BsuRI GGCC 4 cut(s) 62, 165, 247, 260
BsuTUI ATCGAT 1 cut(s) 529
BtgI CCRYGG 1 cut(s) 75
BtsCI GGATG 2 cut(s) 655, 691
BtsIMutI CAGTG 2 cut(s) 228, 379
Cac8I GCNNGC 1 cut(s) 317
CfoI GCGC 2 cut(s) 43, 82
Cfr13I GGNCC 4 cut(s) 61, 245, 246, 352
ClaI ATCGAT 1 cut(s) 529
CseI GACGC 1 cut(s) 27
Csp6I GTAC 1 cut(s) 180
CviAII CATG 2 cut(s) 418, 566
CviQI GTAC 1 cut(s) 180
DdeI CTNAG 4 cut(s) 14, 311, 617, 681
DinI GGCGCC 1 cut(s) 81
DpnI GATC 3 cut(s) 8, 453, 528
DpnII GATC 3 cut(s) 6, 451, 526
DraI TTTAAA 1 cut(s) 507
Eam1104I CTCTTC 1 cut(s) 622
EarI CTCTTC 1 cut(s) 622
Eco130I CCWWGG 1 cut(s) 498
Eco24I GRGCYC 1 cut(s) 249
Eco47I GGWCC 1 cut(s) 352
Eco81I CCTNAGG 1 cut(s) 681
EcoO109I RGGNCCY 1 cut(s) 245
EcoT14I CCWWGG 1 cut(s) 498
EcoT38I GRGCYC 1 cut(s) 249
EgeI GGCGCC 1 cut(s) 81
EheI GGCGCC 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 498
Esp3I CGTCTC 1 cut(s) 10
FaeI CATG 2 cut(s) 421, 569
FaiI YATR 8 cut(s) 242, 276, 278, 419, 473, 567, 667, 735
FalI AAGNNNNNCTT 2 cut(s) 674, 706
FaqI GGGAC 1 cut(s) 480
FatI CATG 2 cut(s) 417, 565
FauNDI CATATG 1 cut(s) 276
Fnu4HI GCNGC 8 cut(s) 70, 83, 86, 120, 285, 386, 540, 783
FokI GGATG 2 cut(s) 662, 698
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 8 cut(s) 70, 83, 86, 120, 285, 386, 540, 783
GlaI GCGC 2 cut(s) 42, 81
GluI GCNGC 8 cut(s) 70, 83, 86, 120, 285, 386, 540, 783
HaeII RGCGCY 1 cut(s) 83
HaeIII GGCC 4 cut(s) 62, 165, 247, 260
HapII CCGG 4 cut(s) 49, 59, 236, 341
HgaI GACGC 1 cut(s) 27
HhaI GCGC 2 cut(s) 43, 82
Hin1I GRCGYC 1 cut(s) 80
Hin1II CATG 2 cut(s) 421, 569
Hin6I GCGC 2 cut(s) 41, 80
HinP1I GCGC 2 cut(s) 41, 80
HincII GTYRAC 1 cut(s) 592
HindII GTYRAC 1 cut(s) 592
HinfI GANTC 5 cut(s) 24, 54, 209, 394, 422
HpaI GTTAAC 1 cut(s) 592
HpaII CCGG 4 cut(s) 49, 59, 236, 341
Hpy166II GTNNAC 1 cut(s) 592
Hpy188I TCNGA 4 cut(s) 15, 35, 214, 399
Hpy188III TCNNGA 2 cut(s) 125, 739
Hpy8I GTNNAC 1 cut(s) 592
HpyAV CCTTC 1 cut(s) 766
HpyCH4III ACNGT 2 cut(s) 76, 223
HpyCH4IV ACGT 1 cut(s) 431
HpyCH4V TGCA 4 cut(s) 119, 539, 653, 759
HpyF10VI GCNNNNNNNGC 4 cut(s) 49, 293, 618, 650
HpyF3I CTNAG 4 cut(s) 14, 311, 617, 681
HpySE526I ACGT 1 cut(s) 431
Hsp92I GRCGYC 1 cut(s) 80
Hsp92II CATG 2 cut(s) 421, 569
HspAI GCGC 2 cut(s) 41, 80
KasI GGCGCC 1 cut(s) 79
KspAI GTTAAC 1 cut(s) 592
Kzo9I GATC 3 cut(s) 6, 451, 526
LmnI GCTCC 2 cut(s) 36, 718
Lsp1109I GCAGC 5 cut(s) 131, 271, 372, 551, 769
LweI GCATC 2 cut(s) 344, 640
MaeII ACGT 1 cut(s) 431
MaeIII GTNAC 1 cut(s) 167
MalI GATC 3 cut(s) 8, 453, 528
MboI GATC 3 cut(s) 6, 451, 526
MboII GAAGA 6 cut(s) 212, 218, 439, 478, 617, 639
MhlI GDGCHC 1 cut(s) 249
MluCI AATT 3 cut(s) 128, 534, 654
Mly113I GGCGCC 1 cut(s) 80
MlyI GAGTC 1 cut(s) 403
MseI TTAA 6 cut(s) 506, 533, 591, 657, 692, 793
MslI CAYNNNNRTG 2 cut(s) 275, 362
MspI CCGG 4 cut(s) 49, 59, 236, 341
MspR9I CCNGG 2 cut(s) 59, 342
Mva1269I GAATGC 1 cut(s) 351
MvnI CGCG 2 cut(s) 21, 41
MwoI GCNNNNNNNGC 4 cut(s) 49, 293, 618, 650
NarI GGCGCC 1 cut(s) 80
NciI CCSGG 2 cut(s) 59, 342
NdeI CATATG 1 cut(s) 276
NdeII GATC 3 cut(s) 6, 451, 526
NlaIII CATG 2 cut(s) 421, 569
NlaIV GGNNCC 4 cut(s) 73, 81, 246, 247
NspI RCATGY 1 cut(s) 569
NspV TTCGAA 1 cut(s) 425
PctI GAATGC 1 cut(s) 351
PfeI GAWTC 4 cut(s) 24, 54, 209, 422
PflFI GACNNNGTC 1 cut(s) 433
PfoI TCCNGGA 1 cut(s) 340
PkrI GCNGC 8 cut(s) 71, 84, 87, 121, 286, 387, 541, 784
PleI GAGTC 1 cut(s) 402
PluTI GGCGCC 1 cut(s) 83
PpsI GAGTC 1 cut(s) 402
PshBI ATTAAT 1 cut(s) 533
PspN4I GGNNCC 4 cut(s) 73, 81, 246, 247
PspOMI GGGCCC 1 cut(s) 245
PspPI GGNCC 4 cut(s) 61, 245, 246, 352
PstI CTGCAG 1 cut(s) 121
PsyI GACNNNGTC 1 cut(s) 433
RsaI GTAC 1 cut(s) 181
RsaNI GTAC 1 cut(s) 180
RseI CAYNNNNRTG 2 cut(s) 275, 362
SaqAI TTAA 6 cut(s) 506, 533, 591, 657, 692, 793
SatI GCNGC 8 cut(s) 70, 83, 86, 120, 285, 386, 540, 783
Sau3AI GATC 3 cut(s) 6, 451, 526
Sau96I GGNCC 4 cut(s) 61, 245, 246, 352
SchI GAGTC 1 cut(s) 403
ScrFI CCNGG 2 cut(s) 59, 342
SduI GDGCHC 1 cut(s) 249
SfaNI GCATC 2 cut(s) 344, 640
SfcI CTRYAG 1 cut(s) 117
SfoI GGCGCC 1 cut(s) 81
SfuI TTCGAA 1 cut(s) 425
SinI GGWCC 1 cut(s) 352
SmiMI CAYNNNNRTG 2 cut(s) 275, 362
Sse9I AATT 3 cut(s) 128, 534, 654
SsiI CCGC 5 cut(s) 21, 69, 83, 86, 404
SspDI GGCGCC 1 cut(s) 79
StyD4I CCNGG 2 cut(s) 57, 340
StyI CCWWGG 1 cut(s) 498
TaaI ACNGT 2 cut(s) 76, 223
TaiI ACGT 1 cut(s) 434
TaqI TCGA 2 cut(s) 425, 529
TasI AATT 3 cut(s) 128, 534, 654
TauI GCSGC 3 cut(s) 72, 85, 88
TfiI GAWTC 4 cut(s) 24, 54, 209, 422
Tru1I TTAA 6 cut(s) 506, 533, 591, 657, 692, 793
Tru9I TTAA 6 cut(s) 506, 533, 591, 657, 692, 793
TscAI CASTG 2 cut(s) 228, 379
TseI GCWGC 5 cut(s) 119, 284, 385, 539, 782
TspDTI ATGAA 1 cut(s) 219
TspGWI ACGGA 2 cut(s) 337, 435
TspRI CASTG 2 cut(s) 228, 379
Tth111I GACNNNGTC 1 cut(s) 433
VpaK11BI GGWCC 1 cut(s) 352
VspI ATTAAT 1 cut(s) 533
XapI RAATTY 1 cut(s) 128
XceI RCATGY 1 cut(s) 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.