MD09G1014900.v1.1

Ribosome-recycling factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0007948
Physical Location & Seq
Reverse (-)
940336 .. 942002
1667 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1014900.v1.1.491

Sequence Viewer

Length: 219 bp
ATGGCAAGCAGAGTATTCGAAGAGCTCGCCAAAAGAAATGTATCATTAGTAGCACTGAAGACCAAAACAGCAATGGATACGGTAAAAAAGTTGACCAAAAACTACTCCAAAGACGACTTAAAAAGATGGGAGAAAGAGGTTGATGAGTTGACTAAAAAGCTTGTCAAGACAGCAGAGGAGTTATGCAAGGCAAAGGAGAAGGAGATCAGTTCAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRF

Protein Analysis

73

Amino Acids

8.26

Weight (kDa)

9.41

Isoelectric Point (pI)

37.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRF PF01765 21 - 69 2.4e-09 Ribosome recycling factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 77
AluBI AGCT 2 cut(s) 25, 160
AluI AGCT 2 cut(s) 25, 160
Alw21I GWGCWC 1 cut(s) 27
AsuII TTCGAA 1 cut(s) 18
BanII GRGCYC 1 cut(s) 27
BbsI GAAGAC 1 cut(s) 65
Bbv12I GWGCWC 1 cut(s) 27
BccI CCATC 1 cut(s) 120
BcgI CGANNNNNNTGC 1 cut(s) 32
BciVI GTATCC 1 cut(s) 70
BfuI GTATCC 1 cut(s) 70
BpiI GAAGAC 1 cut(s) 65
Bpu14I TTCGAA 1 cut(s) 18
Bse3DI GCAATG 1 cut(s) 78
BseMI GCAATG 1 cut(s) 78
BseRI GAGGAG 1 cut(s) 191
BsiHKAI GWGCWC 1 cut(s) 27
Bsp119I TTCGAA 1 cut(s) 18
Bsp1286I GDGCHC 1 cut(s) 27
Bsp143I GATC 1 cut(s) 204
BspQI GCTCTTC 1 cut(s) 15
BspT104I TTCGAA 1 cut(s) 18
BsrDI GCAATG 1 cut(s) 78
BssMI GATC 1 cut(s) 204
Bst4CI ACNGT 1 cut(s) 82
Bst6I CTCTTC 1 cut(s) 15
BstBI TTCGAA 1 cut(s) 18
BstC8I GCNNGC 2 cut(s) 7, 27
BstKTI GATC 1 cut(s) 207
BstMBI GATC 1 cut(s) 204
BstV2I GAAGAC 1 cut(s) 65
BsuI GTATCC 1 cut(s) 70
BtsIMutI CAGTG 1 cut(s) 53
Cac8I GCNNGC 2 cut(s) 7, 27
CviJI RGCY 3 cut(s) 25, 160, 216
CviKI_1 RGCY 3 cut(s) 25, 160, 216
DpnI GATC 1 cut(s) 206
DpnII GATC 1 cut(s) 204
Eam1104I CTCTTC 1 cut(s) 15
EarI CTCTTC 1 cut(s) 15
Ecl136II GAGCTC 1 cut(s) 25
Eco24I GRGCYC 1 cut(s) 27
Eco53kI GAGCTC 1 cut(s) 25
Eco57I CTGAAG 1 cut(s) 77
EcoICRI GAGCTC 1 cut(s) 25
EcoT38I GRGCYC 1 cut(s) 27
FaiI YATR 1 cut(s) 184
FriOI GRGCYC 1 cut(s) 27
HincII GTYRAC 2 cut(s) 93, 150
HindII GTYRAC 2 cut(s) 93, 150
HindIII AAGCTT 1 cut(s) 158
Hpy166II GTNNAC 2 cut(s) 93, 150
Hpy188III TCNNGA 1 cut(s) 166
Hpy8I GTNNAC 2 cut(s) 93, 150
HpyAV CCTTC 1 cut(s) 193
HpyCH4III ACNGT 1 cut(s) 82
HpyCH4V TGCA 1 cut(s) 186
Kzo9I GATC 1 cut(s) 204
LguI GCTCTTC 1 cut(s) 15
LpnPI CCDG 1 cut(s) 198
MalI GATC 1 cut(s) 206
MboI GATC 1 cut(s) 204
MboII GAAGA 2 cut(s) 32, 70
MhlI GDGCHC 1 cut(s) 27
MnlI CCTC 2 cut(s) 130, 169
MseI TTAA 1 cut(s) 119
NdeII GATC 1 cut(s) 204
NspV TTCGAA 1 cut(s) 18
PciSI GCTCTTC 1 cut(s) 15
Psp124BI GAGCTC 1 cut(s) 27
SacI GAGCTC 1 cut(s) 27
SapI GCTCTTC 1 cut(s) 15
SaqAI TTAA 1 cut(s) 119
Sau3AI GATC 1 cut(s) 204
SduI GDGCHC 1 cut(s) 27
SetI ASST 3 cut(s) 27, 141, 162
SfuI TTCGAA 1 cut(s) 18
SgeI CNNG 5 cut(s) 18, 38, 173, 178, 199
SstI GAGCTC 1 cut(s) 27
TaaI ACNGT 1 cut(s) 82
TaqI TCGA 1 cut(s) 18
Tru1I TTAA 1 cut(s) 119
Tru9I TTAA 1 cut(s) 119
TscAI CASTG 1 cut(s) 60
TspRI CASTG 1 cut(s) 60
XcmI CCANNNNNNNNNTGG 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.