Prupe.3G302800_v2.0.a1

Ribosome-recycling factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0007948
Physical Location & Seq
Forward (+)
26650135 .. 26652914
2780 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G302800.1

Sequence Viewer

Length: 777 bp
ATGGCGATCTTTCTGAGACGCGCACTGTCATTCCGAAACGTCCTTCTCCTTCGAAGCTCTGCGATTGTCGGTCGCGAGCTCTTCCAGTACAACTCCGAGGCACCGCAGAGAATTCTGTCTCATTCCGCTCTTGAATTCGTCAAAGAAAGTCGCAGAGGCTTTGCCAAAGGCCGCAATAAATCAAAAGATGACTCGGCTGCGGGCACAATTGAAGTTTTGCCAAATATGGGGCCAACCATTAAGGCAAACACGACCTCGCAAATGGAGGCGGCAATTACAGCATTGTCAGCAGAATTAAGCAAACTACGAACTGGAAGAGCATCTCCGGGAATGCTTGACCATATTATTGTGGAAACCGGTGGCGTCAAGCTGCCCCTGAGTCAGATAGCAGTTGTTTCAGTCCTGGACTCGAAAACATTATCGATAAACCCTTTTGATCCAAATGCCTTACAGAAGTTGGAGACTGCCATTGTTGAATCTGCCTTGGGGTTAAATCCTAAAGTGGATGGGGATCGATTGATTGCAGCTATTCCTCCATTGACCAAAGAGCATGTTCAGGCTGTATGTAAAGTGGTTACCAAGTCCTGTGAAGATGGCAGACTAAGTATAAGAAGAGCTCGCCAAAAGGCAATGGATACAATAAAAAAGTTGTATTCAAGATACCCAAAGGACGACTTAAAAAGATTGGAGAAAGAGGTTGAGGAGTTGACTAAAAAGTTTGTGAAGACAGCAGAGGACTTGTGCAAGGCAAAGGAGAAGGAGATCAGTGCAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRF

Protein Analysis

259

Amino Acids

28.37

Weight (kDa)

9.64

Isoelectric Point (pI)

40.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 100
AccBSI CCGCTC 1 cut(s) 128
AccII CGCG 2 cut(s) 21, 75
AciI CCGC 5 cut(s) 104, 126, 172, 200, 269
AclWI GGATC 2 cut(s) 431, 519
AcsI RAATTY 2 cut(s) 111, 134
AcyI GRCGYC 1 cut(s) 363
AfaI GTAC 1 cut(s) 89
AfiI CCNNNNNNNGG 1 cut(s) 227
AgeI ACCGGT 1 cut(s) 356
AgsI TTSAA 4 cut(s) 134, 212, 476, 657
AjnI CCWGG 1 cut(s) 402
AluBI AGCT 6 cut(s) 57, 79, 370, 527, 617, 774
AluI AGCT 6 cut(s) 57, 79, 370, 527, 617, 774
Alw21I GWGCWC 2 cut(s) 81, 619
Alw26I GTCTC 3 cut(s) 10, 123, 455
AlwI GGATC 2 cut(s) 431, 519
AoxI GGCC 2 cut(s) 169, 230
ApeKI GCWGC 3 cut(s) 197, 370, 524
ApoI RAATTY 2 cut(s) 111, 134
AsiGI ACCGGT 1 cut(s) 356
AspLEI GCGC 1 cut(s) 23
AspS9I GGNCC 1 cut(s) 230
AsuC2I CCSGG 1 cut(s) 327
AsuII TTCGAA 1 cut(s) 52
BaeGI GKGCMC 1 cut(s) 206
BanI GGYRCC 1 cut(s) 100
BanII GRGCYC 2 cut(s) 81, 619
BbsI GAAGAC 1 cut(s) 731
Bbv12I GWGCWC 2 cut(s) 81, 619
BbvI GCAGC 3 cut(s) 184, 357, 536
BccI CCATC 2 cut(s) 500, 587
BciT130I CCWGG 1 cut(s) 404
BciVI GTATCC 1 cut(s) 628
BcnI CCSGG 1 cut(s) 327
BcoDI GTCTC 3 cut(s) 10, 123, 455
BfuI GTATCC 1 cut(s) 628
BisI GCNGC 5 cut(s) 172, 198, 270, 371, 525
BlsI GCNGC 5 cut(s) 173, 199, 271, 372, 526
Bme1390I CCNGG 2 cut(s) 327, 404
BmgT120I GGNCC 1 cut(s) 230
BmiI GGNNCC 2 cut(s) 102, 231
BmrFI CCNGG 2 cut(s) 327, 404
BmsI GCATC 1 cut(s) 329
BpiI GAAGAC 1 cut(s) 731
Bpu14I TTCGAA 1 cut(s) 52
BpuMI CCSGG 1 cut(s) 327
Bsa29I ATCGAT 2 cut(s) 422, 514
BsaBI GATNNNNATC 1 cut(s) 510
BsaHI GRCGYC 1 cut(s) 363
BsaJI CCNNGG 2 cut(s) 96, 483
BsaWI WCCGGW 1 cut(s) 356
Bsc4I CCNNNNNNNGG 1 cut(s) 227
Bse118I RCCGGY 1 cut(s) 356
Bse1I ACTGG 2 cut(s) 85, 316
Bse3DI GCAATG 1 cut(s) 636
Bse8I GATNNNNATC 1 cut(s) 510
BseBI CCWGG 1 cut(s) 404
BseCI ATCGAT 2 cut(s) 422, 514
BseDI CCNNGG 2 cut(s) 96, 483
BseGI GGATG 1 cut(s) 511
BseJI GATNNNNATC 1 cut(s) 510
BseLI CCNNNNNNNGG 1 cut(s) 227
BseMI GCAATG 1 cut(s) 636
BseMII CTCAG 2 cut(s) 5, 368
BseNI ACTGG 2 cut(s) 85, 316
BseRI GAGGAG 1 cut(s) 716
BseSI GKGCMC 1 cut(s) 206
BseXI GCAGC 3 cut(s) 184, 357, 536
Bsh1236I CGCG 2 cut(s) 21, 75
Bsh1285I CGRYCG 1 cut(s) 73
BshFI GGCC 2 cut(s) 171, 232
BshNI GGYRCC 1 cut(s) 100
BshTI ACCGGT 1 cut(s) 356
BshVI ATCGAT 2 cut(s) 422, 514
BsiEI CGRYCG 1 cut(s) 73
BsiHKAI GWGCWC 2 cut(s) 81, 619
BsiSI CCGG 2 cut(s) 326, 357
BslI CCNNNNNNNGG 1 cut(s) 227
BsmAI GTCTC 3 cut(s) 10, 123, 455
BsmBI CGTCTC 1 cut(s) 10
BsmI GAATGC 1 cut(s) 336
BsnI GGCC 2 cut(s) 171, 232
Bsp119I TTCGAA 1 cut(s) 52
Bsp1286I GDGCHC 3 cut(s) 81, 206, 619
Bsp143I GATC 4 cut(s) 6, 436, 511, 762
Bsp68I TCGCGA 1 cut(s) 75
BspACI CCGC 5 cut(s) 104, 126, 172, 200, 269
BspANI GGCC 2 cut(s) 171, 232
BspCNI CTCAG 2 cut(s) 6, 369
BspDI ATCGAT 2 cut(s) 422, 514
BspFNI CGCG 2 cut(s) 21, 75
BspLI GGNNCC 2 cut(s) 102, 231
BspPI GGATC 2 cut(s) 431, 519
BspQI GCTCTTC 3 cut(s) 86, 310, 607
BspT104I TTCGAA 1 cut(s) 52
BspT107I GGYRCC 1 cut(s) 100
BsrBI CCGCTC 1 cut(s) 128
BsrDI GCAATG 1 cut(s) 636
BsrFI RCCGGY 1 cut(s) 356
BsrI ACTGG 2 cut(s) 85, 316
BssAI RCCGGY 1 cut(s) 356
BssECI CCNNGG 2 cut(s) 96, 483
BssMI GATC 4 cut(s) 6, 436, 511, 762
BssNI GRCGYC 1 cut(s) 363
BssT1I CCWWGG 1 cut(s) 483
Bst2UI CCWGG 1 cut(s) 404
Bst4CI ACNGT 1 cut(s) 27
Bst6I CTCTTC 3 cut(s) 86, 310, 607
BstACI GRCGYC 1 cut(s) 363
BstBI TTCGAA 1 cut(s) 52
BstC8I GCNNGC 4 cut(s) 77, 202, 619, 772
BstDEI CTNAG 3 cut(s) 14, 377, 602
BstEII GGTNACC 1 cut(s) 574
BstF5I GGATG 1 cut(s) 511
BstFNI CGCG 2 cut(s) 21, 75
BstHHI GCGC 1 cut(s) 23
BstKTI GATC 4 cut(s) 9, 439, 514, 765
BstMAI GTCTC 3 cut(s) 10, 123, 455
BstMBI GATC 4 cut(s) 6, 436, 511, 762
BstMCI CGRYCG 1 cut(s) 73
BstMWI GCNNNNNNNGC 2 cut(s) 278, 287
BstNI CCWGG 1 cut(s) 404
BstNSI RCATGY 1 cut(s) 554
BstPI GGTNACC 1 cut(s) 574
BstSCI CCNGG 2 cut(s) 325, 402
BstSLI GKGCMC 1 cut(s) 206
BstUI CGCG 2 cut(s) 21, 75
BstV1I GCAGC 3 cut(s) 184, 357, 536
BstV2I GAAGAC 1 cut(s) 731
Bsu15I ATCGAT 2 cut(s) 422, 514
BsuI GTATCC 1 cut(s) 628
BsuRI GGCC 2 cut(s) 171, 232
BsuTUI ATCGAT 2 cut(s) 422, 514
BtsCI GGATG 1 cut(s) 511
BtsIMutI CAGTG 2 cut(s) 23, 772
BtuMI TCGCGA 1 cut(s) 75
Cac8I GCNNGC 4 cut(s) 77, 202, 619, 772
CfoI GCGC 1 cut(s) 23
Cfr10I RCCGGY 1 cut(s) 356
Cfr13I GGNCC 1 cut(s) 230
ClaI ATCGAT 2 cut(s) 422, 514
CseI GACGC 2 cut(s) 27, 352
Csp6I GTAC 1 cut(s) 88
CspAI ACCGGT 1 cut(s) 356
CviAII CATG 1 cut(s) 551
CviQI GTAC 1 cut(s) 88
DdeI CTNAG 3 cut(s) 14, 377, 602
DpnI GATC 4 cut(s) 8, 438, 513, 764
DpnII GATC 4 cut(s) 6, 436, 511, 762
Eam1104I CTCTTC 3 cut(s) 86, 310, 607
EarI CTCTTC 3 cut(s) 86, 310, 607
Ecl136II GAGCTC 2 cut(s) 79, 617
Eco130I CCWWGG 1 cut(s) 483
Eco24I GRGCYC 2 cut(s) 81, 619
Eco53kI GAGCTC 2 cut(s) 79, 617
Eco91I GGTNACC 1 cut(s) 574
EcoICRI GAGCTC 2 cut(s) 79, 617
EcoO65I GGTNACC 1 cut(s) 574
EcoRI GAATTC 2 cut(s) 111, 134
EcoRII CCWGG 1 cut(s) 402
EcoT14I CCWWGG 1 cut(s) 483
EcoT38I GRGCYC 2 cut(s) 81, 619
ErhI CCWWGG 1 cut(s) 483
Esp3I CGTCTC 1 cut(s) 10
FaeI CATG 1 cut(s) 554
FaiI YATR 5 cut(s) 227, 342, 552, 565, 608
FalI AAGNNNNNCTT 2 cut(s) 659, 691
FatI CATG 1 cut(s) 550
FauI CCCGC 1 cut(s) 193
Fnu4HI GCNGC 5 cut(s) 172, 198, 270, 371, 525
FokI GGATG 1 cut(s) 518
FriOI GRGCYC 2 cut(s) 81, 619
Fsp4HI GCNGC 5 cut(s) 172, 198, 270, 371, 525
GlaI GCGC 1 cut(s) 22
GluI GCNGC 5 cut(s) 172, 198, 270, 371, 525
HaeIII GGCC 2 cut(s) 171, 232
HapII CCGG 2 cut(s) 326, 357
HgaI GACGC 2 cut(s) 27, 352
HhaI GCGC 1 cut(s) 23
Hin1I GRCGYC 1 cut(s) 363
Hin1II CATG 1 cut(s) 554
Hin6I GCGC 1 cut(s) 21
HinP1I GCGC 1 cut(s) 21
HincII GTYRAC 1 cut(s) 708
HindII GTYRAC 1 cut(s) 708
HinfI GANTC 4 cut(s) 191, 379, 407, 476
HpaII CCGG 2 cut(s) 326, 357
Hpy166II GTNNAC 1 cut(s) 708
Hpy188I TCNGA 4 cut(s) 15, 35, 97, 384
Hpy188III TCNNGA 3 cut(s) 74, 131, 657
Hpy8I GTNNAC 1 cut(s) 708
HpyAV CCTTC 3 cut(s) 53, 59, 751
HpyCH4III ACNGT 1 cut(s) 27
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 3 cut(s) 524, 744, 770
HpyF10VI GCNNNNNNNGC 2 cut(s) 278, 287
HpyF3I CTNAG 3 cut(s) 14, 377, 602
HpySE526I ACGT 1 cut(s) 39
Hsp92I GRCGYC 1 cut(s) 363
Hsp92II CATG 1 cut(s) 554
HspAI GCGC 1 cut(s) 21
Kzo9I GATC 4 cut(s) 6, 436, 511, 762
LguI GCTCTTC 3 cut(s) 86, 310, 607
LpnPI CCDG 9 cut(s) 98, 297, 339, 370, 389, 389, 416, 542, 598
Lsp1109I GCAGC 3 cut(s) 184, 357, 536
LweI GCATC 1 cut(s) 329
MaeII ACGT 1 cut(s) 39
MaeIII GTNAC 1 cut(s) 574
MalI GATC 4 cut(s) 8, 438, 513, 764
MbiI CCGCTC 1 cut(s) 128
MboI GATC 4 cut(s) 6, 436, 511, 762
MboII GAAGA 5 cut(s) 73, 327, 602, 624, 736
MfeI CAATTG 1 cut(s) 207
MhlI GDGCHC 3 cut(s) 81, 206, 619
MluCI AATT 5 cut(s) 111, 134, 207, 273, 293
MlyI GAGTC 3 cut(s) 185, 388, 401
MmeI TCCRAC 1 cut(s) 438
MnlI CCTC 8 cut(s) 91, 149, 259, 265, 543, 688, 694, 727
MseI TTAA 4 cut(s) 240, 296, 491, 677
MspI CCGG 2 cut(s) 326, 357
MspR9I CCNGG 2 cut(s) 327, 404
MunI CAATTG 1 cut(s) 207
Mva1269I GAATGC 1 cut(s) 336
MvaI CCWGG 1 cut(s) 404
MvnI CGCG 2 cut(s) 21, 75
MwoI GCNNNNNNNGC 2 cut(s) 278, 287
NciI CCSGG 1 cut(s) 327
NdeII GATC 4 cut(s) 6, 436, 511, 762
NlaIII CATG 1 cut(s) 554
NlaIV GGNNCC 2 cut(s) 102, 231
NmeAIII GCCGAG 1 cut(s) 173
NruI TCGCGA 1 cut(s) 75
NspI RCATGY 1 cut(s) 554
NspV TTCGAA 1 cut(s) 52
PciSI GCTCTTC 3 cut(s) 86, 310, 607
PctI GAATGC 1 cut(s) 336
PfeI GAWTC 1 cut(s) 476
PfoI TCCNGGA 2 cut(s) 325, 402
PinAI ACCGGT 1 cut(s) 356
PkrI GCNGC 5 cut(s) 173, 199, 271, 372, 526
PleI GAGTC 3 cut(s) 185, 387, 401
PpsI GAGTC 3 cut(s) 185, 387, 401
Psp124BI GAGCTC 2 cut(s) 81, 619
Psp6I CCWGG 1 cut(s) 402
PspEI GGTNACC 1 cut(s) 574
PspGI CCWGG 1 cut(s) 402
PspN4I GGNNCC 2 cut(s) 102, 231
PspPI GGNCC 1 cut(s) 230
RruI TCGCGA 1 cut(s) 75
RsaI GTAC 1 cut(s) 89
RsaNI GTAC 1 cut(s) 88
SacI GAGCTC 2 cut(s) 81, 619
SapI GCTCTTC 3 cut(s) 86, 310, 607
SaqAI TTAA 4 cut(s) 240, 296, 491, 677
SatI GCNGC 5 cut(s) 172, 198, 270, 371, 525
Sau3AI GATC 4 cut(s) 6, 436, 511, 762
Sau96I GGNCC 1 cut(s) 230
SchI GAGTC 3 cut(s) 185, 388, 401
ScrFI CCNGG 2 cut(s) 327, 404
SduI GDGCHC 3 cut(s) 81, 206, 619
SetI ASST 9 cut(s) 42, 59, 81, 257, 372, 529, 619, 699, 776
SfaNI GCATC 1 cut(s) 329
SfuI TTCGAA 1 cut(s) 52
Sse9I AATT 5 cut(s) 111, 134, 207, 273, 293
SsiI CCGC 5 cut(s) 104, 126, 172, 200, 269
SstI GAGCTC 2 cut(s) 81, 619
StyD4I CCNGG 2 cut(s) 325, 402
StyI CCWWGG 1 cut(s) 483
TaaI ACNGT 1 cut(s) 27
TaiI ACGT 1 cut(s) 42
TaqI TCGA 4 cut(s) 52, 410, 422, 514
TaqII GACCGA 1 cut(s) 59
TasI AATT 5 cut(s) 111, 134, 207, 273, 293
TatI WGTACW 1 cut(s) 87
TauI GCSGC 2 cut(s) 174, 272
TfiI GAWTC 1 cut(s) 476
Tru1I TTAA 4 cut(s) 240, 296, 491, 677
Tru9I TTAA 4 cut(s) 240, 296, 491, 677
TscAI CASTG 2 cut(s) 30, 772
TseI GCWGC 3 cut(s) 197, 370, 524
TspRI CASTG 2 cut(s) 30, 772
XapI RAATTY 2 cut(s) 111, 134
XceI RCATGY 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.