AT3G02770

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2- oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0011677
Physical Location & Seq
Reverse (-)
600849 .. 602399
1551 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G02770.1

Sequence Viewer

Length: 501 bp
ATGGCGTTTGTGACCACTGCGGAAGTGTGTGACGCGAATCAAGAGATGATTCGAAGTGGCCAGCTCCGAGCTCTGCAACCCGTTTTTCAGATTTATGGTCGTCGTCAAATATTTTCAGGTCCAGTAGTTACTGTTAAAGTCTTTGAAGACAATGGCTTAATCCGTCACTTCCTCGAAGAGAAAGGCAATGGTAGAGTTCTTGTGGTGGATGGTGGAGGGAGTTTACGCTGTGCGATACTAGGAGGAAACCCGGTCGTACAAGCGCAGAACAACGGATGGGCAGGGATCATAGTGAATGGATGCATCAGAGACGTTGATGAGATCAATGGTTGCGACATTGGAGTGAGAGCTTTAGCTTCTCATCCAATAAAGGCTAGTAAGAAAGGACTTGGTGAACAAAGAGTCTCTTTGAACATTGCAGGCACTCGGATATGCGATGGCGAATGGCTTTATGCAGATACTGATGGTATCCTTGTTTCTCAGATTGAATTATCCGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

166

Amino Acids

17.84

Weight (kDa)

5.68

Isoelectric Point (pI)

33.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RraA-like PF03737 6 - 159 2.6e-45 Aldolase/RraA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 35
AciI CCGC 1 cut(s) 20
AclWI GGATC 1 cut(s) 293
AcoI YGGCCR 1 cut(s) 58
AfaI GTAC 1 cut(s) 258
AgsI TTSAA 3 cut(s) 146, 412, 488
AluBI AGCT 4 cut(s) 64, 71, 350, 356
AluI AGCT 4 cut(s) 64, 71, 350, 356
Alw21I GWGCWC 1 cut(s) 73
Alw26I GTCTC 2 cut(s) 303, 409
AlwI GGATC 1 cut(s) 293
AlwNI CAGNNNCTG 1 cut(s) 461
AoxI GGCC 1 cut(s) 58
AspLEI GCGC 1 cut(s) 265
AspS9I GGNCC 1 cut(s) 119
AsuC2I CCSGG 1 cut(s) 251
AsuHPI GGTGA 1 cut(s) 404
AsuII TTCGAA 1 cut(s) 52
AvaII GGWCC 1 cut(s) 119
BalI TGGCCA 1 cut(s) 60
BanII GRGCYC 1 cut(s) 73
BbsI GAAGAC 1 cut(s) 153
Bbv12I GWGCWC 1 cut(s) 73
BccI CCATC 4 cut(s) 203, 270, 431, 458
BciVI GTATCC 1 cut(s) 479
BcnI CCSGG 1 cut(s) 251
BcoDI GTCTC 2 cut(s) 303, 409
BfaI CTAG 2 cut(s) 239, 375
BfuI GTATCC 1 cut(s) 479
Bme1390I CCNGG 1 cut(s) 251
Bme18I GGWCC 1 cut(s) 119
BmgT120I GGNCC 1 cut(s) 119
BmrFI CCNGG 1 cut(s) 251
BmsI GCATC 2 cut(s) 290, 312
BpiI GAAGAC 1 cut(s) 153
Bpu14I TTCGAA 1 cut(s) 52
BpuMI CCSGG 1 cut(s) 251
Bse1I ACTGG 1 cut(s) 122
Bse3DI GCAATG 2 cut(s) 193, 414
BseGI GGATG 4 cut(s) 214, 281, 305, 361
BseMI GCAATG 2 cut(s) 193, 414
BseMII CTCAG 1 cut(s) 494
BseNI ACTGG 1 cut(s) 122
Bsh1236I CGCG 1 cut(s) 35
Bsh1285I CGRYCG 1 cut(s) 255
BshFI GGCC 1 cut(s) 60
BsiEI CGRYCG 1 cut(s) 255
BsiHKAI GWGCWC 1 cut(s) 73
BsiSI CCGG 1 cut(s) 251
BsmAI GTCTC 2 cut(s) 303, 409
BsmBI CGTCTC 1 cut(s) 303
BsnI GGCC 1 cut(s) 60
Bsp119I TTCGAA 1 cut(s) 52
Bsp1286I GDGCHC 1 cut(s) 73
Bsp143I GATC 2 cut(s) 285, 321
BspACI CCGC 1 cut(s) 20
BspANI GGCC 1 cut(s) 60
BspCNI CTCAG 1 cut(s) 493
BspFNI CGCG 1 cut(s) 35
BspPI GGATC 1 cut(s) 293
BspT104I TTCGAA 1 cut(s) 52
BsrDI GCAATG 2 cut(s) 193, 414
BsrI ACTGG 1 cut(s) 122
BssMI GATC 2 cut(s) 285, 321
Bst4CI ACNGT 1 cut(s) 133
Bst6I CTCTTC 1 cut(s) 171
BstBI TTCGAA 1 cut(s) 52
BstC8I GCNNGC 2 cut(s) 62, 421
BstDEI CTNAG 1 cut(s) 480
BstF5I GGATG 4 cut(s) 214, 281, 305, 361
BstFNI CGCG 1 cut(s) 35
BstHHI GCGC 1 cut(s) 265
BstKTI GATC 2 cut(s) 288, 324
BstMAI GTCTC 2 cut(s) 303, 409
BstMBI GATC 2 cut(s) 285, 321
BstMCI CGRYCG 1 cut(s) 255
BstSCI CCNGG 1 cut(s) 249
BstUI CGCG 1 cut(s) 35
BstV2I GAAGAC 1 cut(s) 153
BsuI GTATCC 1 cut(s) 479
BsuRI GGCC 1 cut(s) 60
BtgZI GCGATG 1 cut(s) 450
BtsCI GGATG 4 cut(s) 214, 281, 305, 361
BtsI GCAGTG 1 cut(s) 15
BtsIMutI CAGTG 1 cut(s) 15
Cac8I GCNNGC 2 cut(s) 62, 421
CaiI CAGNNNCTG 1 cut(s) 461
CfoI GCGC 1 cut(s) 265
Cfr13I GGNCC 1 cut(s) 119
CseI GACGC 1 cut(s) 41
Csp6I GTAC 1 cut(s) 257
CviJI RGCY 8 cut(s) 60, 64, 71, 156, 350, 356, 374, 448
CviKI_1 RGCY 8 cut(s) 60, 64, 71, 156, 350, 356, 374, 448
CviQI GTAC 1 cut(s) 257
DdeI CTNAG 1 cut(s) 480
DpnI GATC 2 cut(s) 287, 323
DpnII GATC 2 cut(s) 285, 321
EaeI YGGCCR 1 cut(s) 58
Eam1104I CTCTTC 1 cut(s) 171
EarI CTCTTC 1 cut(s) 171
Ecl136II GAGCTC 1 cut(s) 71
Eco24I GRGCYC 1 cut(s) 73
Eco47I GGWCC 1 cut(s) 119
Eco53kI GAGCTC 1 cut(s) 71
EcoICRI GAGCTC 1 cut(s) 71
EcoT22I ATGCAT 1 cut(s) 305
EcoT38I GRGCYC 1 cut(s) 73
Esp3I CGTCTC 1 cut(s) 303
FaiI YATR 4 cut(s) 96, 290, 433, 453
FalI AAGNNNNNCTT 2 cut(s) 391, 423
FokI GGATG 4 cut(s) 221, 288, 312, 348
FriOI GRGCYC 1 cut(s) 73
FspBI CTAG 2 cut(s) 239, 375
GlaI GCGC 1 cut(s) 264
HaeIII GGCC 1 cut(s) 60
HapII CCGG 1 cut(s) 251
HgaI GACGC 1 cut(s) 41
HhaI GCGC 1 cut(s) 265
Hin6I GCGC 1 cut(s) 263
HinP1I GCGC 1 cut(s) 263
HinfI GANTC 3 cut(s) 37, 49, 402
HpaII CCGG 1 cut(s) 251
HphI GGTGA 1 cut(s) 404
Hpy166II GTNNAC 2 cut(s) 224, 395
Hpy188I TCNGA 5 cut(s) 68, 90, 308, 429, 483
Hpy188III TCNNGA 1 cut(s) 41
Hpy8I GTNNAC 2 cut(s) 224, 395
Hpy99I CGWCG 1 cut(s) 105
HpyCH4III ACNGT 1 cut(s) 133
HpyCH4IV ACGT 1 cut(s) 312
HpyCH4V TGCA 4 cut(s) 76, 303, 419, 455
HpyF3I CTNAG 1 cut(s) 480
HpySE526I ACGT 1 cut(s) 312
HspAI GCGC 1 cut(s) 263
Kzo9I GATC 2 cut(s) 285, 321
LmnI GCTCC 1 cut(s) 69
LpnPI CCDG 6 cut(s) 74, 102, 135, 264, 267, 405
LweI GCATC 2 cut(s) 290, 312
MaeI CTAG 2 cut(s) 239, 375
MaeII ACGT 1 cut(s) 312
MaeIII GTNAC 4 cut(s) 10, 29, 127, 164
MalI GATC 2 cut(s) 287, 323
MboI GATC 2 cut(s) 285, 321
MboII GAAGA 2 cut(s) 158, 188
MhlI GDGCHC 1 cut(s) 73
MlsI TGGCCA 1 cut(s) 60
MluCI AATT 1 cut(s) 488
MluNI TGGCCA 1 cut(s) 60
MlyI GAGTC 1 cut(s) 411
MnlI CCTC 3 cut(s) 182, 209, 236
Mox20I TGGCCA 1 cut(s) 60
Mph1103I ATGCAT 1 cut(s) 305
MscI TGGCCA 1 cut(s) 60
MseI TTAA 3 cut(s) 135, 158, 499
MslI CAYNNNNRTG 1 cut(s) 341
Msp20I TGGCCA 1 cut(s) 60
MspI CCGG 1 cut(s) 251
MspR9I CCNGG 1 cut(s) 251
MvnI CGCG 1 cut(s) 35
NciI CCSGG 1 cut(s) 251
NdeII GATC 2 cut(s) 285, 321
NmuCI GTSAC 3 cut(s) 10, 29, 164
NsiI ATGCAT 1 cut(s) 305
NspV TTCGAA 1 cut(s) 52
PfeI GAWTC 2 cut(s) 37, 49
PleI GAGTC 1 cut(s) 410
PpsI GAGTC 1 cut(s) 410
Psp124BI GAGCTC 1 cut(s) 73
PspPI GGNCC 1 cut(s) 119
PstNI CAGNNNCTG 1 cut(s) 461
RsaI GTAC 1 cut(s) 258
RsaNI GTAC 1 cut(s) 257
RseI CAYNNNNRTG 1 cut(s) 341
SacI GAGCTC 1 cut(s) 73
SaqAI TTAA 3 cut(s) 135, 158, 499
Sau3AI GATC 2 cut(s) 285, 321
Sau96I GGNCC 1 cut(s) 119
SchI GAGTC 1 cut(s) 411
ScrFI CCNGG 1 cut(s) 251
SduI GDGCHC 1 cut(s) 73
SetI ASST 6 cut(s) 66, 73, 121, 315, 352, 358
SfaNI GCATC 2 cut(s) 290, 312
SfuI TTCGAA 1 cut(s) 52
SinI GGWCC 1 cut(s) 119
SmiMI CAYNNNNRTG 1 cut(s) 341
Sse9I AATT 1 cut(s) 488
SsiI CCGC 1 cut(s) 20
SspI AATATT 1 cut(s) 111
SspMI CTAG 2 cut(s) 239, 375
SstI GAGCTC 1 cut(s) 73
StyD4I CCNGG 1 cut(s) 249
TaaI ACNGT 1 cut(s) 133
TaiI ACGT 1 cut(s) 315
TaqI TCGA 2 cut(s) 52, 174
TasI AATT 1 cut(s) 488
TfiI GAWTC 2 cut(s) 37, 49
Tru1I TTAA 3 cut(s) 135, 158, 499
Tru9I TTAA 3 cut(s) 135, 158, 499
TscAI CASTG 1 cut(s) 22
TseFI GTSAC 3 cut(s) 10, 29, 164
Tsp45I GTSAC 3 cut(s) 10, 29, 164
TspGWI ACGGA 3 cut(s) 152, 288, 484
TspRI CASTG 1 cut(s) 22
VpaK11BI GGWCC 1 cut(s) 119
XspI CTAG 2 cut(s) 239, 375
Zsp2I ATGCAT 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.