Rorug02G0277200

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2- oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001919
Physical Location & Seq
Forward (+)
29848605 .. 29852549
3945 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0277200.1

Sequence Viewer

Length: 999 bp
ATGGCAATCTCAATCTCCTCCTCCTGCTTTGTTCCTCTTCTATCTCTGAACACTGTCTCCTCTCCTTCTTCGTTTCCATTTTCTCTGAGTTGTGTGAGGCCTCAGAGCCGGAGAAATCAGCGCTTGGTGGTTAGCTGCTCGTCTTCTGAAACCGACTCCGGCTCTGACTCACTCAATGGAAATGGAAGACATTCCCAAATGTCTTCTATGACTCCTTTTGGGGTGACAATAAATGAAGCTGGGTTGTCCAGACCACCTTATAAATGGCGAAGGGTCTTGCTTAAAGTAAGCGGAGAAGCACTTGCCGGAGATCACACACAGAATATTGACCCGAAGGTAACAATGGAAATTGCAAGGGAAGTTGCATCTGTGACTCGCCTTGGCATTGAGGTTGCCATTGTTGTTGGTGGGGGAAATATATTCCGTGGAGCATCCTGGGCTGGAAGTAGTGGTCTTGACCGTTCATCTGCTGATTACATCGGGATGTTGGCTACTGTAATGAATTCAATATTTCTCCAAGCCACAATGGAGAGTATTGGTATCCCGACTCGAGTGCAGACTGCATTTCGGATGTCTGAGGTTGCAGAACCTTATATACGTCGAAGGGCTGTGAGGCATCTGGAAAAGGGCAGGGTAGTGATCTTTGCAGCAGGAACTGGAAATCCATTCTTTACAACAGATACTGCTGCAGCACTTCGTGGTGCAGAAATCAATGCAGAGGTGGTGCTGAAAGCTACAAATGTTGATGGAGTTTATGATGAGGATCCTAGGTGTAACCCAAATGCCTGTCTTCTTGATACTCTTACATATCAGGAGGTGACATCAAAAGATCTTTCAGTGATGGACATGACTGCTATTACTTTATGCCAGGAGAACAAAATTCCTGTTGTTGTGTTCAATCTAAACAAACCAGGTAACATCTCAAAAGCCATAAAGGGGGAGAAGGTTGGCACGTTAATTGGGGCAGCATGGAATTCCACAGTGGCGCCAAGAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

332

Amino Acids

35.58

Weight (kDa)

8.75

Isoelectric Point (pI)

46.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AA_kinase PF00696 91 - 300 1.9e-36 Amino acid kinase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 261
AccB1I GGYRCC 1 cut(s) 985
AciI CCGC 1 cut(s) 291
AclWI GGATC 2 cut(s) 758, 771
AcsI RAATTY 3 cut(s) 502, 879, 973
AcyI GRCGYC 1 cut(s) 986
AdeI CACNNNGTG 1 cut(s) 698
AfeI AGCGCT 1 cut(s) 122
AfiI CCNNNNNNNGG 1 cut(s) 936
AgsI TTSAA 2 cut(s) 507, 898
AjnI CCWGG 3 cut(s) 434, 867, 910
AloI GAACNNNNNNTCC 4 cut(s) 41, 73, 646, 678
AluBI AGCT 3 cut(s) 135, 239, 734
AluI AGCT 3 cut(s) 135, 239, 734
Alw26I GTCTC 1 cut(s) 61
AlwI GGATC 2 cut(s) 758, 771
AlwNI CAGNNNCTG 2 cut(s) 656, 683
Ama87I CYCGRG 1 cut(s) 549
Aor51HI AGCGCT 1 cut(s) 122
AoxI GGCC 1 cut(s) 98
ApeKI GCWGC 5 cut(s) 135, 647, 686, 689, 965
ApoI RAATTY 3 cut(s) 502, 879, 973
Asp700I GAANNNNTTC 1 cut(s) 190
AspA2I CCTAGG 1 cut(s) 767
AspLEI GCGC 2 cut(s) 123, 988
AsuHPI GGTGA 2 cut(s) 235, 829
AvaI CYCGRG 1 cut(s) 549
AvrII CCTAGG 1 cut(s) 767
BamHI GGATCC 1 cut(s) 763
BanI GGYRCC 1 cut(s) 985
BbsI GAAGAC 4 cut(s) 135, 193, 195, 782
BbvI GCAGC 5 cut(s) 122, 659, 673, 701, 977
BccI CCATC 2 cut(s) 740, 835
BcgI CGANNNNNNTGC 2 cut(s) 535, 569
BciT130I CCWGG 3 cut(s) 436, 869, 912
BciVI GTATCC 1 cut(s) 551
BcoDI GTCTC 1 cut(s) 61
BfaI CTAG 1 cut(s) 768
BfmI CTRYAG 1 cut(s) 687
BfoI RGCGCY 2 cut(s) 124, 989
BfuI GTATCC 1 cut(s) 551
BglII AGATCT 1 cut(s) 829
BisI GCNGC 5 cut(s) 136, 648, 687, 690, 966
BlnI CCTAGG 1 cut(s) 767
BlsI GCNGC 5 cut(s) 137, 649, 688, 691, 967
Bme1390I CCNGG 3 cut(s) 436, 869, 912
BmeT110I CYCGRG 1 cut(s) 549
BmiI GGNNCC 2 cut(s) 765, 987
BmrFI CCNGG 3 cut(s) 436, 869, 912
BmsI GCATC 3 cut(s) 374, 440, 625
BpiI GAAGAC 4 cut(s) 135, 193, 195, 782
BsaBI GATNNNNATC 1 cut(s) 762
BsaHI GRCGYC 1 cut(s) 986
BsaJI CCNNGG 4 cut(s) 379, 424, 435, 767
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 1 cut(s) 936
Bse1I ACTGG 1 cut(s) 661
Bse8I GATNNNNATC 1 cut(s) 762
BseBI CCWGG 3 cut(s) 436, 869, 912
BseDI CCNNGG 4 cut(s) 379, 424, 435, 767
BseGI GGATG 3 cut(s) 431, 489, 576
BseJI GATNNNNATC 1 cut(s) 762
BseLI CCNNNNNNNGG 1 cut(s) 936
BseMII CTCAG 3 cut(s) 77, 116, 567
BseNI ACTGG 1 cut(s) 661
BseRI GAGGAG 3 cut(s) 7, 10, 49
BseXI GCAGC 5 cut(s) 122, 659, 673, 701, 977
BseYI CCCAGC 1 cut(s) 239
BsgI GTGCAG 2 cut(s) 575, 723
BshFI GGCC 1 cut(s) 100
BshNI GGYRCC 1 cut(s) 985
BsiHKCI CYCGRG 1 cut(s) 549
BsiSI CCGG 3 cut(s) 109, 159, 306
BslI CCNNNNNNNGG 1 cut(s) 936
BsmAI GTCTC 1 cut(s) 61
BsnI GGCC 1 cut(s) 100
BsoBI CYCGRG 1 cut(s) 549
Bsp143I GATC 4 cut(s) 310, 639, 763, 829
BspACI CCGC 1 cut(s) 291
BspANI GGCC 1 cut(s) 100
BspCNI CTCAG 3 cut(s) 78, 115, 568
BspLI GGNNCC 2 cut(s) 765, 987
BspMAI CTGCAG 1 cut(s) 691
BspPI GGATC 2 cut(s) 758, 771
BspT107I GGYRCC 1 cut(s) 985
BsrI ACTGG 1 cut(s) 661
BssECI CCNNGG 4 cut(s) 379, 424, 435, 767
BssMI GATC 4 cut(s) 310, 639, 763, 829
BssNI GRCGYC 1 cut(s) 986
BssT1I CCWWGG 2 cut(s) 379, 767
Bst2UI CCWGG 3 cut(s) 436, 869, 912
Bst4CI ACNGT 4 cut(s) 55, 461, 496, 982
Bst6I CTCTTC 1 cut(s) 42
BstACI GRCGYC 1 cut(s) 986
BstDEI CTNAG 3 cut(s) 86, 102, 576
BstDSI CCRYGG 1 cut(s) 424
BstF5I GGATG 3 cut(s) 431, 489, 576
BstH2I RGCGCY 2 cut(s) 124, 989
BstHHI GCGC 2 cut(s) 123, 988
BstKTI GATC 4 cut(s) 313, 642, 766, 832
BstMAI GTCTC 1 cut(s) 61
BstMBI GATC 4 cut(s) 310, 639, 763, 829
BstMWI GCNNNNNNNGC 1 cut(s) 437
BstNI CCWGG 3 cut(s) 436, 869, 912
BstSCI CCNGG 3 cut(s) 434, 867, 910
BstSFI CTRYAG 1 cut(s) 687
BstV1I GCAGC 5 cut(s) 122, 659, 673, 701, 977
BstV2I GAAGAC 4 cut(s) 135, 193, 195, 782
BstX2I RGATCY 2 cut(s) 763, 829
BstYI RGATCY 2 cut(s) 763, 829
BsuI GTATCC 1 cut(s) 551
BsuRI GGCC 1 cut(s) 100
BtgI CCRYGG 1 cut(s) 424
BtsCI GGATG 3 cut(s) 431, 489, 576
BtsIMutI CAGTG 3 cut(s) 51, 843, 987
CaiI CAGNNNCTG 2 cut(s) 656, 683
CfoI GCGC 2 cut(s) 123, 988
CsiI ACCWGGT 1 cut(s) 910
CviAII CATG 3 cut(s) 847, 969, 996
DdeI CTNAG 3 cut(s) 86, 102, 576
DinI GGCGCC 1 cut(s) 987
DpnI GATC 4 cut(s) 312, 641, 765, 831
DpnII GATC 4 cut(s) 310, 639, 763, 829
DraIII CACNNNGTG 1 cut(s) 698
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
Eco130I CCWWGG 2 cut(s) 379, 767
Eco147I AGGCCT 1 cut(s) 100
Eco47III AGCGCT 1 cut(s) 122
Eco88I CYCGRG 1 cut(s) 549
EcoRI GAATTC 2 cut(s) 502, 973
EcoRII CCWGG 3 cut(s) 434, 867, 910
EcoT14I CCWWGG 2 cut(s) 379, 767
EgeI GGCGCC 1 cut(s) 987
EheI GGCGCC 1 cut(s) 987
ErhI CCWWGG 2 cut(s) 379, 767
FaeI CATG 3 cut(s) 850, 972, 999
FatI CATG 3 cut(s) 846, 968, 995
Fnu4HI GCNGC 5 cut(s) 136, 648, 687, 690, 966
FokI GGATG 3 cut(s) 418, 496, 583
Fsp4HI GCNGC 5 cut(s) 136, 648, 687, 690, 966
FspBI CTAG 1 cut(s) 768
GlaI GCGC 2 cut(s) 122, 987
GluI GCNGC 5 cut(s) 136, 648, 687, 690, 966
GsaI CCCAGC 1 cut(s) 243
HaeII RGCGCY 2 cut(s) 124, 989
HaeIII GGCC 1 cut(s) 100
HapII CCGG 3 cut(s) 109, 159, 306
HhaI GCGC 2 cut(s) 123, 988
Hin1I GRCGYC 1 cut(s) 986
Hin1II CATG 3 cut(s) 850, 972, 999
Hin6I GCGC 2 cut(s) 121, 986
HinP1I GCGC 2 cut(s) 121, 986
HinfI GANTC 5 cut(s) 155, 167, 211, 373, 547
HpaII CCGG 3 cut(s) 109, 159, 306
HphI GGTGA 2 cut(s) 235, 829
Hpy188I TCNGA 7 cut(s) 48, 87, 105, 148, 166, 570, 577
Hpy188III TCNNGA 7 cut(s) 249, 455, 481, 544, 620, 794, 812
Hpy99I CGWCG 1 cut(s) 603
HpyAV CCTTC 5 cut(s) 75, 264, 328, 597, 937
HpyCH4III ACNGT 4 cut(s) 55, 461, 496, 982
HpyCH4IV ACGT 2 cut(s) 598, 953
HpyCH4V TGCA 9 cut(s) 353, 365, 556, 563, 584, 647, 689, 704, 716
HpyF10VI GCNNNNNNNGC 1 cut(s) 437
HpyF3I CTNAG 3 cut(s) 86, 102, 576
HpySE526I ACGT 2 cut(s) 598, 953
Hsp92I GRCGYC 1 cut(s) 986
Hsp92II CATG 3 cut(s) 850, 972, 999
HspAI GCGC 2 cut(s) 121, 986
KasI GGCGCC 1 cut(s) 985
Kzo9I GATC 4 cut(s) 310, 639, 763, 829
LmnI GCTCC 1 cut(s) 428
Lsp1109I GCAGC 5 cut(s) 122, 659, 673, 701, 977
LweI GCATC 3 cut(s) 374, 440, 625
MabI ACCWGGT 1 cut(s) 910
MaeI CTAG 1 cut(s) 768
MaeII ACGT 2 cut(s) 598, 953
MaeIII GTNAC 6 cut(s) 223, 337, 370, 773, 817, 914
MalI GATC 4 cut(s) 312, 641, 765, 831
MboI GATC 4 cut(s) 310, 639, 763, 829
MboII GAAGA 6 cut(s) 29, 60, 135, 195, 198, 782
MflI RGATCY 2 cut(s) 763, 829
MluCI AATT 5 cut(s) 348, 502, 879, 957, 973
Mly113I GGCGCC 1 cut(s) 986
MlyI GAGTC 5 cut(s) 149, 161, 205, 367, 541
MroXI GAANNNNTTC 1 cut(s) 190
MseI TTAA 2 cut(s) 282, 956
MslI CAYNNNNRTG 1 cut(s) 482
MspI CCGG 3 cut(s) 109, 159, 306
MspR9I CCNGG 3 cut(s) 436, 869, 912
MvaI CCWGG 3 cut(s) 436, 869, 912
MwoI GCNNNNNNNGC 1 cut(s) 437
NarI GGCGCC 1 cut(s) 986
NdeII GATC 4 cut(s) 310, 639, 763, 829
NlaIII CATG 3 cut(s) 850, 972, 999
NlaIV GGNNCC 2 cut(s) 765, 987
NmuCI GTSAC 3 cut(s) 223, 370, 817
PaeR7I CTCGAG 1 cut(s) 549
PceI AGGCCT 1 cut(s) 100
PdmI GAANNNNTTC 1 cut(s) 190
PkrI GCNGC 5 cut(s) 137, 649, 688, 691, 967
PleI GAGTC 5 cut(s) 149, 161, 205, 367, 541
PluTI GGCGCC 1 cut(s) 989
PpsI GAGTC 5 cut(s) 149, 161, 205, 367, 541
PsiI TTATAA 1 cut(s) 261
Psp6I CCWGG 3 cut(s) 434, 867, 910
PspFI CCCAGC 1 cut(s) 239
PspGI CCWGG 3 cut(s) 434, 867, 910
PspN4I GGNNCC 2 cut(s) 765, 987
PspXI VCTCGAGB 1 cut(s) 549
PsrI GAACNNNNNNTAC 2 cut(s) 579, 611
PstI CTGCAG 1 cut(s) 691
PstNI CAGNNNCTG 2 cut(s) 656, 683
PsuI RGATCY 2 cut(s) 763, 829
RseI CAYNNNNRTG 1 cut(s) 482
SaqAI TTAA 2 cut(s) 282, 956
SatI GCNGC 5 cut(s) 136, 648, 687, 690, 966
Sau3AI GATC 4 cut(s) 310, 639, 763, 829
SchI GAGTC 5 cut(s) 149, 161, 205, 367, 541
ScrFI CCNGG 3 cut(s) 436, 869, 912
SexAI ACCWGGT 1 cut(s) 910
SfaNI GCATC 3 cut(s) 374, 440, 625
SfcI CTRYAG 1 cut(s) 687
SfoI GGCGCC 1 cut(s) 987
Sfr274I CTCGAG 1 cut(s) 549
SlaI CTCGAG 1 cut(s) 549
SmiMI CAYNNNNRTG 1 cut(s) 482
SmlI CTYRAG 1 cut(s) 549
SmoI CTYRAG 1 cut(s) 549
Sse9I AATT 5 cut(s) 348, 502, 879, 957, 973
SseBI AGGCCT 1 cut(s) 100
SsiI CCGC 1 cut(s) 291
SspDI GGCGCC 1 cut(s) 985
SspI AATATT 2 cut(s) 325, 510
SspMI CTAG 1 cut(s) 768
StuI AGGCCT 1 cut(s) 100
StyD4I CCNGG 3 cut(s) 434, 867, 910
StyI CCWWGG 2 cut(s) 379, 767
TaaI ACNGT 4 cut(s) 55, 461, 496, 982
TaiI ACGT 2 cut(s) 601, 956
TaqI TCGA 2 cut(s) 550, 601
TasI AATT 5 cut(s) 348, 502, 879, 957, 973
Tru1I TTAA 2 cut(s) 282, 956
Tru9I TTAA 2 cut(s) 282, 956
TscAI CASTG 3 cut(s) 58, 843, 987
TseFI GTSAC 3 cut(s) 223, 370, 817
TseI GCWGC 5 cut(s) 135, 647, 686, 689, 965
Tsp45I GTSAC 3 cut(s) 223, 370, 817
TspDTI ATGAA 3 cut(s) 249, 453, 515
TspGWI ACGGA 1 cut(s) 413
TspRI CASTG 3 cut(s) 58, 843, 987
XapI RAATTY 3 cut(s) 502, 879, 973
XcmI CCANNNNNNNNNTGG 1 cut(s) 261
XhoI CTCGAG 1 cut(s) 549
XmaJI CCTAGG 1 cut(s) 767
XmnI GAANNNNTTC 1 cut(s) 190
XspI CTAG 1 cut(s) 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.