MD17G1082700.v1.1

meiotic DNA integrity checkpoint

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001919
Physical Location & Seq
Reverse (-)
6892068 .. 6892923
856 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1082700.v1.1.491

Sequence Viewer

Length: 426 bp
ATGGCGGACGACGACATCTTCGGCGAAGTTTTTTTGAATTTGGAAGCGAACCACCAAAAGGAAGGCTTCGACGAAGGCTACAGAGACGGCTTGGTCGCCGGAAAGGAGGAGGCGAAGCCGACGGGGATCAAAACTGGGTTCGAGGTGGGCGAGGAGCTTGGGTTCTACAAGGGATGCGTGGATGTATGGAACTCCGCGATCCGGGTCGATCCGACACGGTTCTCGCACCGGGTCCAGAAGACCATCAGGCAGATGGGGGAGCTGATTGAGAAGTACCCGATCATGGAACCCGAGGACGAGAGCGTCGGGGATGTGATGGAGGCTCTGAGGTTGAAGTTCCGGGCGGTTTGTGCTTCGTTGGGTGTGAAATTGGAGTATAAGGGGTACCCAAAAGCTGCTTCTGAGGCTACGGAGACGTCGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000076 GO:0000077 GO:0000228 GO:0000278 GO:0000724 GO:0000725 GO:0000793 GO:0000794 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006281 GO:0006289 GO:0006302 GO:0006310 GO:0006412 GO:0006413 GO:0006518 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010389 GO:0010467 GO:0010564 GO:0010948 GO:0010972 GO:0019538 GO:0022402 GO:0022414 GO:0022613 GO:0030896 GO:0031570 GO:0031573 GO:0031974 GO:0031981 GO:0032991 GO:0033313 GO:0033314 GO:0033554 GO:0034641 GO:0034645 GO:0035861 GO:0042254 GO:0042273 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044773 GO:0044774 GO:0044778 GO:0044818 GO:0045786 GO:0045930 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051321 GO:0051604 GO:0051716 GO:0051726 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0090734 GO:0097428 GO:0106035 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902749 GO:1902750 GO:1903046 GO:1903047
KEGG Pathways
Metabolic & Signaling
ko00260 ko00350 ko00360 ko00410 ko00950 ko00960 ko01100 ko01110 ko04218 map00260 map00350 map00360 map00410 map00950 map00960 map01100 map01110 map04218
Pfam Domains
Protein Families
FTCD_N Yae1_N

Protein Analysis

142

Amino Acids

15.78

Weight (kDa)

4.79

Isoelectric Point (pI)

20.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Yae1_N PF09811 22 - 60 8.3e-08 Essential protein Yae1, N terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 92, 302
AatII GACGTC 1 cut(s) 419
Acc65I GGTACC 1 cut(s) 384
AccB1I GGYRCC 1 cut(s) 384
AccII CGCG 1 cut(s) 197
AciI CCGC 3 cut(s) 5, 195, 344
AclWI GGATC 3 cut(s) 134, 193, 203
AcsI RAATTY 1 cut(s) 37
AcyI GRCGYC 1 cut(s) 416
AfaI GTAC 2 cut(s) 275, 386
AfiI CCNNNNNNNGG 3 cut(s) 58, 201, 283
AgsI TTSAA 2 cut(s) 37, 334
AjuI GAANNNNNNNTTGG 2 cut(s) 382, 414
AloI GAACNNNNNNTCC 2 cut(s) 146, 178
AluBI AGCT 3 cut(s) 157, 262, 395
AluI AGCT 3 cut(s) 157, 262, 395
Alw26I GTCTC 2 cut(s) 78, 407
AlwI GGATC 3 cut(s) 134, 193, 203
Ama87I CYCGRG 1 cut(s) 290
ApeKI GCWGC 1 cut(s) 395
ApoI RAATTY 1 cut(s) 37
ArsI GACNNNNNNTTYG 1 cut(s) 34
Asp718I GGTACC 1 cut(s) 384
AspS9I GGNCC 1 cut(s) 232
AsuC2I CCSGG 3 cut(s) 203, 230, 341
AvaI CYCGRG 1 cut(s) 290
AvaII GGWCC 1 cut(s) 232
BanI GGYRCC 1 cut(s) 384
BbsI GAAGAC 1 cut(s) 245
BbvI GCAGC 1 cut(s) 382
BccI CCATC 3 cut(s) 247, 251, 310
BceAI ACGGC 1 cut(s) 103
BcnI CCSGG 3 cut(s) 203, 230, 341
BcoDI GTCTC 2 cut(s) 78, 407
BfmI CTRYAG 1 cut(s) 79
BisI GCNGC 1 cut(s) 396
BlsI GCNGC 1 cut(s) 397
Bme1390I CCNGG 3 cut(s) 203, 230, 341
Bme18I GGWCC 1 cut(s) 232
BmeT110I CYCGRG 1 cut(s) 290
BmgT120I GGNCC 1 cut(s) 232
BmiI GGNNCC 3 cut(s) 233, 288, 386
BmrFI CCNGG 3 cut(s) 203, 230, 341
BmrI ACTGGG 1 cut(s) 144
BmsI GCATC 1 cut(s) 164
BmuI ACTGGG 1 cut(s) 144
BpiI GAAGAC 1 cut(s) 245
BpuMI CCSGG 3 cut(s) 203, 230, 341
BsaHI GRCGYC 1 cut(s) 416
BsaJI CCNNGG 1 cut(s) 291
BsaXI ACNNNNNCTCC 3 cut(s) 146, 176, 404
Bsc4I CCNNNNNNNGG 3 cut(s) 58, 201, 283
Bse1I ACTGG 1 cut(s) 139
BseDI CCNNGG 1 cut(s) 291
BseGI GGATG 3 cut(s) 179, 187, 316
BseLI CCNNNNNNNGG 3 cut(s) 58, 201, 283
BseMII CTCAG 2 cut(s) 317, 393
BseNI ACTGG 1 cut(s) 139
BseRI GAGGAG 2 cut(s) 122, 167
BseXI GCAGC 1 cut(s) 382
Bsh1236I CGCG 1 cut(s) 197
BshNI GGYRCC 1 cut(s) 384
BsiHKCI CYCGRG 1 cut(s) 290
BsiSI CCGG 4 cut(s) 99, 202, 229, 340
BslI CCNNNNNNNGG 3 cut(s) 58, 201, 283
BsmAI GTCTC 2 cut(s) 78, 407
BsmBI CGTCTC 2 cut(s) 78, 407
BsoBI CYCGRG 1 cut(s) 290
Bsp143I GATC 4 cut(s) 126, 198, 208, 279
BspACI CCGC 3 cut(s) 5, 195, 344
BspCNI CTCAG 2 cut(s) 318, 394
BspFNI CGCG 1 cut(s) 197
BspLI GGNNCC 3 cut(s) 233, 288, 386
BspPI GGATC 3 cut(s) 134, 193, 203
BspT107I GGYRCC 1 cut(s) 384
BsrI ACTGG 1 cut(s) 139
BssECI CCNNGG 1 cut(s) 291
BssMI GATC 4 cut(s) 126, 198, 208, 279
BssNI GRCGYC 1 cut(s) 416
Bst4CI ACNGT 1 cut(s) 219
BstACI GRCGYC 1 cut(s) 416
BstDEI CTNAG 2 cut(s) 326, 402
BstF5I GGATG 3 cut(s) 179, 187, 316
BstFNI CGCG 1 cut(s) 197
BstKTI GATC 4 cut(s) 129, 201, 211, 282
BstMAI GTCTC 2 cut(s) 78, 407
BstMBI GATC 4 cut(s) 126, 198, 208, 279
BstMWI GCNNNNNNNGC 2 cut(s) 350, 404
BstSCI CCNGG 3 cut(s) 201, 228, 339
BstSFI CTRYAG 1 cut(s) 79
BstUI CGCG 1 cut(s) 197
BstV1I GCAGC 1 cut(s) 382
BstV2I GAAGAC 1 cut(s) 245
BtsCI GGATG 3 cut(s) 179, 187, 316
Cfr13I GGNCC 1 cut(s) 232
CseI GACGC 1 cut(s) 292
Csp6I GTAC 2 cut(s) 274, 385
CviAII CATG 1 cut(s) 283
CviJI RGCY 9 cut(s) 66, 78, 90, 118, 157, 262, 323, 395, 407
CviKI_1 RGCY 9 cut(s) 66, 78, 90, 118, 157, 262, 323, 395, 407
CviQI GTAC 2 cut(s) 274, 385
DdeI CTNAG 2 cut(s) 326, 402
DpnI GATC 4 cut(s) 128, 200, 210, 281
DpnII GATC 4 cut(s) 126, 198, 208, 279
DrdI GACNNNNNNGTC 2 cut(s) 92, 302
DseDI GACNNNNNNGTC 2 cut(s) 92, 302
EciI GGCGGA 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 232
Eco88I CYCGRG 1 cut(s) 290
Esp3I CGTCTC 2 cut(s) 78, 407
FaeI CATG 1 cut(s) 286
FaiI YATR 3 cut(s) 187, 284, 378
FalI AAGNNNNNCTT 2 cut(s) 50, 82
FatI CATG 1 cut(s) 282
Fnu4HI GCNGC 1 cut(s) 396
FokI GGATG 3 cut(s) 186, 194, 323
Fsp4HI GCNGC 1 cut(s) 396
GluI GCNGC 1 cut(s) 396
HapII CCGG 4 cut(s) 99, 202, 229, 340
HgaI GACGC 1 cut(s) 292
Hin1I GRCGYC 1 cut(s) 416
Hin1II CATG 1 cut(s) 286
HpaII CCGG 4 cut(s) 99, 202, 229, 340
Hpy188I TCNGA 3 cut(s) 213, 327, 403
Hpy188III TCNNGA 1 cut(s) 235
Hpy99I CGWCG 5 cut(s) 14, 74, 124, 308, 421
HpyAV CCTTC 2 cut(s) 56, 68
HpyCH4III ACNGT 1 cut(s) 219
HpyCH4IV ACGT 1 cut(s) 416
HpyF10VI GCNNNNNNNGC 2 cut(s) 350, 404
HpyF3I CTNAG 2 cut(s) 326, 402
HpySE526I ACGT 1 cut(s) 416
Hsp92I GRCGYC 1 cut(s) 416
Hsp92II CATG 1 cut(s) 286
KpnI GGTACC 1 cut(s) 388
Kzo9I GATC 4 cut(s) 126, 198, 208, 279
LmnI GCTCC 2 cut(s) 154, 259
LpnPI CCDG 7 cut(s) 112, 120, 215, 232, 242, 248, 353
Lsp1109I GCAGC 1 cut(s) 382
LweI GCATC 1 cut(s) 164
MaeII ACGT 1 cut(s) 416
MalI GATC 4 cut(s) 128, 200, 210, 281
MboI GATC 4 cut(s) 126, 198, 208, 279
MboII GAAGA 2 cut(s) 10, 250
MluCI AATT 2 cut(s) 37, 368
MmeI TCCRAC 1 cut(s) 236
MnlI CCTC 8 cut(s) 100, 103, 136, 145, 286, 313, 321, 397
MspI CCGG 4 cut(s) 99, 202, 229, 340
MspR9I CCNGG 3 cut(s) 203, 230, 341
MvnI CGCG 1 cut(s) 197
MwoI GCNNNNNNNGC 2 cut(s) 350, 404
NciI CCSGG 3 cut(s) 203, 230, 341
NdeII GATC 4 cut(s) 126, 198, 208, 279
NlaIII CATG 1 cut(s) 286
NlaIV GGNNCC 3 cut(s) 233, 288, 386
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 416
PkrI GCNGC 1 cut(s) 397
PspN4I GGNNCC 3 cut(s) 233, 288, 386
PspPI GGNCC 1 cut(s) 232
RsaI GTAC 2 cut(s) 275, 386
RsaNI GTAC 2 cut(s) 274, 385
SatI GCNGC 1 cut(s) 396
Sau3AI GATC 4 cut(s) 126, 198, 208, 279
Sau96I GGNCC 1 cut(s) 232
ScrFI CCNGG 3 cut(s) 203, 230, 341
SetI ASST 6 cut(s) 147, 159, 264, 332, 397, 419
SfaNI GCATC 1 cut(s) 164
SfcI CTRYAG 1 cut(s) 79
SinI GGWCC 1 cut(s) 232
Sse9I AATT 2 cut(s) 37, 368
SsiI CCGC 3 cut(s) 5, 195, 344
StyD4I CCNGG 3 cut(s) 201, 228, 339
TaaI ACNGT 1 cut(s) 219
TaiI ACGT 1 cut(s) 419
TaqI TCGA 3 cut(s) 69, 141, 207
TasI AATT 2 cut(s) 37, 368
TseI GCWGC 1 cut(s) 395
TspGWI ACGGA 1 cut(s) 425
VpaK11BI GGWCC 1 cut(s) 232
XapI RAATTY 1 cut(s) 37
XcmI CCANNNNNNNNNTGG 1 cut(s) 250
ZraI GACGTC 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.