MD04G1245200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001919
Physical Location & Seq
Forward (+)
32092473 .. 32093094
622 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1245200.v1.1.491

Sequence Viewer

Length: 234 bp
ATGCCAAGAGAGTTGAGTCCGGGCTTGAAGATCCTCTGGGTCTGGACACTTGGCACCGCCGCCGTACTGGTCACCAGTGTGGTGAGGACGAGGATGAGAGACATGGAGAATCTTATCAACACCGAGCAGCAGCAGCCGCAGCCGCAGCAACGCGACACTGCCGCCGACACGGAGACACTCATGGAAGACACCTCGACTCAGTCCAATGAACTGATTCGAGAAGATAAACCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

78

Amino Acids

8.81

Weight (kDa)

4.61

Isoelectric Point (pI)

42.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 53
AccII CGCG 1 cut(s) 153
AciI CCGC 5 cut(s) 57, 60, 137, 143, 162
AclWI GGATC 1 cut(s) 25
AfaI GTAC 1 cut(s) 66
AgsI TTSAA 1 cut(s) 28
AleI CACNNNNGTG 1 cut(s) 77
Alw26I GTCTC 2 cut(s) 93, 167
AlwI GGATC 1 cut(s) 25
ApeKI GCWGC 5 cut(s) 127, 130, 133, 139, 145
Asp700I GAANNNNTTC 1 cut(s) 213
AsuC2I CCSGG 1 cut(s) 21
AsuHPI GGTGA 2 cut(s) 64, 94
BanI GGYRCC 1 cut(s) 53
BbsI GAAGAC 1 cut(s) 192
BbvI GCAGC 5 cut(s) 139, 142, 145, 151, 157
BceAI ACGGC 1 cut(s) 47
BcnI CCSGG 1 cut(s) 21
BcoDI GTCTC 2 cut(s) 93, 167
BisI GCNGC 9 cut(s) 60, 128, 131, 134, 137, 140, 143, 146, 162
BlsI GCNGC 9 cut(s) 61, 129, 132, 135, 138, 141, 144, 147, 163
Bme1390I CCNGG 1 cut(s) 21
BmiI GGNNCC 1 cut(s) 55
BmrFI CCNGG 1 cut(s) 21
BpiI GAAGAC 1 cut(s) 192
BpuMI CCSGG 1 cut(s) 21
Bse1I ACTGG 2 cut(s) 72, 75
BseGI GGATG 1 cut(s) 99
BseMII CTCAG 1 cut(s) 212
BseNI ACTGG 2 cut(s) 72, 75
BseXI GCAGC 5 cut(s) 139, 142, 145, 151, 157
Bsh1236I CGCG 1 cut(s) 153
BshNI GGYRCC 1 cut(s) 53
BsiSI CCGG 1 cut(s) 20
BsmAI GTCTC 2 cut(s) 93, 167
Bsp143I GATC 1 cut(s) 30
BspACI CCGC 5 cut(s) 57, 60, 137, 143, 162
BspCNI CTCAG 1 cut(s) 211
BspFNI CGCG 1 cut(s) 153
BspLI GGNNCC 1 cut(s) 55
BspPI GGATC 1 cut(s) 25
BspT107I GGYRCC 1 cut(s) 53
BsrI ACTGG 2 cut(s) 72, 75
BssMI GATC 1 cut(s) 30
Bst4CI ACNGT 1 cut(s) 231
BstDEI CTNAG 1 cut(s) 198
BstEII GGTNACC 1 cut(s) 70
BstF5I GGATG 1 cut(s) 99
BstFNI CGCG 1 cut(s) 153
BstKTI GATC 1 cut(s) 33
BstMAI GTCTC 2 cut(s) 93, 167
BstMBI GATC 1 cut(s) 30
BstMWI GCNNNNNNNGC 5 cut(s) 133, 136, 139, 142, 145
BstPI GGTNACC 1 cut(s) 70
BstSCI CCNGG 1 cut(s) 19
BstUI CGCG 1 cut(s) 153
BstV1I GCAGC 5 cut(s) 139, 142, 145, 151, 157
BstV2I GAAGAC 1 cut(s) 192
BstX2I RGATCY 1 cut(s) 30
BstYI RGATCY 1 cut(s) 30
BtsCI GGATG 1 cut(s) 99
BtsI GCAGTG 1 cut(s) 156
BtsIMutI CAGTG 2 cut(s) 82, 156
Csp6I GTAC 1 cut(s) 65
CviAII CATG 2 cut(s) 103, 181
CviJI RGCY 3 cut(s) 24, 136, 142
CviKI_1 RGCY 3 cut(s) 24, 136, 142
CviQI GTAC 1 cut(s) 65
DdeI CTNAG 1 cut(s) 198
DpnI GATC 1 cut(s) 32
DpnII GATC 1 cut(s) 30
Eco91I GGTNACC 1 cut(s) 70
EcoO65I GGTNACC 1 cut(s) 70
FaeI CATG 2 cut(s) 106, 184
FaiI YATR 2 cut(s) 104, 182
FatI CATG 2 cut(s) 102, 180
Fnu4HI GCNGC 9 cut(s) 60, 128, 131, 134, 137, 140, 143, 146, 162
FokI GGATG 1 cut(s) 106
Fsp4HI GCNGC 9 cut(s) 60, 128, 131, 134, 137, 140, 143, 146, 162
GluI GCNGC 9 cut(s) 60, 128, 131, 134, 137, 140, 143, 146, 162
HapII CCGG 1 cut(s) 20
Hin1II CATG 2 cut(s) 106, 184
HinfI GANTC 4 cut(s) 16, 109, 196, 214
HpaII CCGG 1 cut(s) 20
HphI GGTGA 2 cut(s) 64, 94
Hpy188III TCNNGA 2 cut(s) 43, 218
HpyCH4III ACNGT 1 cut(s) 231
HpyF10VI GCNNNNNNNGC 5 cut(s) 133, 136, 139, 142, 145
HpyF3I CTNAG 1 cut(s) 198
Hsp92II CATG 2 cut(s) 106, 184
Kzo9I GATC 1 cut(s) 30
LpnPI CCDG 5 cut(s) 22, 28, 33, 53, 88
Lsp1109I GCAGC 5 cut(s) 139, 142, 145, 151, 157
MaeIII GTNAC 1 cut(s) 70
MalI GATC 1 cut(s) 32
MboI GATC 1 cut(s) 30
MboII GAAGA 3 cut(s) 40, 197, 233
MflI RGATCY 1 cut(s) 30
MlyI GAGTC 2 cut(s) 25, 190
MnlI CCTC 4 cut(s) 44, 78, 84, 202
MroXI GAANNNNTTC 1 cut(s) 213
MslI CAYNNNNRTG 1 cut(s) 77
MspI CCGG 1 cut(s) 20
MspR9I CCNGG 1 cut(s) 21
MvnI CGCG 1 cut(s) 153
MwoI GCNNNNNNNGC 5 cut(s) 133, 136, 139, 142, 145
NciI CCSGG 1 cut(s) 21
NdeII GATC 1 cut(s) 30
NlaIII CATG 2 cut(s) 106, 184
NlaIV GGNNCC 1 cut(s) 55
NmuCI GTSAC 1 cut(s) 70
OliI CACNNNNGTG 1 cut(s) 77
PdmI GAANNNNTTC 1 cut(s) 213
PfeI GAWTC 2 cut(s) 109, 214
PflFI GACNNNGTC 1 cut(s) 199
PkrI GCNGC 9 cut(s) 61, 129, 132, 135, 138, 141, 144, 147, 163
PleI GAGTC 2 cut(s) 24, 190
PpsI GAGTC 2 cut(s) 24, 190
PspEI GGTNACC 1 cut(s) 70
PspN4I GGNNCC 1 cut(s) 55
PsuI RGATCY 1 cut(s) 30
PsyI GACNNNGTC 1 cut(s) 199
RsaI GTAC 1 cut(s) 66
RsaNI GTAC 1 cut(s) 65
RseI CAYNNNNRTG 1 cut(s) 77
SatI GCNGC 9 cut(s) 60, 128, 131, 134, 137, 140, 143, 146, 162
Sau3AI GATC 1 cut(s) 30
SchI GAGTC 2 cut(s) 25, 190
ScrFI CCNGG 1 cut(s) 21
SetI ASST 1 cut(s) 194
SmiMI CAYNNNNRTG 1 cut(s) 77
SsiI CCGC 5 cut(s) 57, 60, 137, 143, 162
StyD4I CCNGG 1 cut(s) 19
TaaI ACNGT 1 cut(s) 231
TaqI TCGA 2 cut(s) 194, 217
TauI GCSGC 4 cut(s) 62, 139, 145, 164
TfiI GAWTC 2 cut(s) 109, 214
TscAI CASTG 2 cut(s) 82, 163
TseFI GTSAC 1 cut(s) 70
TseI GCWGC 5 cut(s) 127, 130, 133, 139, 145
Tsp45I GTSAC 1 cut(s) 70
TspDTI ATGAA 1 cut(s) 222
TspGWI ACGGA 1 cut(s) 185
TspRI CASTG 2 cut(s) 82, 163
Tth111I GACNNNGTC 1 cut(s) 199
XmnI GAANNNNTTC 1 cut(s) 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.