Rorug02G0277000

Domain of unknown function (DUF4283)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000109
Physical Location & Seq
Forward (+)
29837039 .. 29842731
5693 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0277000.1

Sequence Viewer

Length: 2034 bp
ATGCTGAGTTCCGATGGGGGCGAGAAGGATTTGTTCTTTGATTCCGCAGAGTATTTGTCATCTGAAGAGTCAGTGGTAGTGAAGGAGGAGTTGGAGTATGAGATTTGGTTGAATGAGCCACCGAGTGTGAAGGAAAGACGAGAGAGTTTTCTACGGCAGATGGGTTTAGCTGAATCTGGATCTACAAAGTTTGGTTCTGTGGAGATGGAAAGGGACGTTGGTACCTCATCGGAGATGATGGGATTGGAGAGACTTTTGGAGTGGAGTGGAGCTGCCTCGAGCTCTTGCAGCTCCTCCTCTAGTCACATTGAGGAATATTCGGGTTTTCATAGCAGGGAAGGGGACAATCTAGCAAATTTTATGTATGATGAATTGGACAGAGACCTGCAAGACAAGTCAAATTTAGTTTCCATTCTACAGTCAGTACAAGTTTCGACTTCTGGTAGTGATTGTTCTCAGGGGGGAACTTGTTCTGAGAGAGAAACTGAGGCTACTGAAGAAGAATGCGAGAGTTCAAAGGAGGGCCGGAAGAAGAAGAGAAGTTGGTGGAAACAGTTTATAAACAAGAGCAAGAGGAGAGGAGGTAAAGTTGTCTCAGAGGTATCAAAACCTGATACTGAAGTATCCAAAGAAAACAGAATGAAGATCAAACAGAACAGTAAGAGGTACATGGAGTTTGCTGCACTTTGCACCGGGCAAGAAATTCAGGCTCACAATGGGTTTATTTGGACGATGAAGTTTAGTCCTGATGGCCAATACATTGCAACTGGTGGTGAAGATGGGGTTGTGCGCATTTGGTGTGTTACAGCAGATGCCTCTTGTAATTACCTTATGGCTCAAGGCAACTTAGATAGCAAACTTAAAAAGATCAAGTCTGGATGTCGAGTTATTTTTCCTGACAACAGTTTTCGAATTGAAGAGTCACCTCTCCAAGAGTTTCTTGGCCATTCCAGTGATGTGTTAGACCTGGCTTGGTCAAATTCAAACCGTCTCATTTCTTCGTCCATGGATAAAACTGTTCGTCTGTGGGAAGTTGGTTGCAGTCAGTGCCTACATGTTTTCCATCACAATGACTATGTTACATGCATTCAGTTCAATCCTCTCGATGACAATTACTTCATCAGTGGATCCATAGATGGAAAGGTTCGAGTTTGGGGGCTTTCTGAGAAGCGAGTTGTTGACTGGGCTGATGTGCGAGATGTCATAACAGCTATTTGTTATCAACCAGATGGGAAGGGTTTCGCAGTTGGTTCCCTTGCAGGCACTTGCCGCTTCTATGAAGAATCAGGAAAAGAAGCTCTTCAGCTTGTTGCACAGATTCATATTCGTTCTAGAAGGAAGATCTCTGGCAACAAAATCACCGGCATCCAGTTTTCCGAGGAAAATGCTCAAAGAGTTATGATAACATCAGAAGACTCAAAAGTCCGTGTGTTTGATGGGGTAGATCTCATTAAGAAATACAGAGGTCTTCCAAAGTCAGGAAGTCAAATGTCAGCTTCTTTCACATCCAGCGGGAAACGTATAATCTCAGTTGGAGAGGACTCGCGCGTTTATTTATGGGACTATGACAGCTGTTGTGTTCCATCATCCAAGCATACAAAATCTGTTCGATCCTGCGAACATTTCTATTGCGAAGGTGTGTCTGTTGCAATCCCTTGGTCAGGCATGGGAACAGAAGATAGGAAATTAGAAACTGCTAGTCCACGATATTGCTCACAAACACAAGGCCGAATAGACGCTACTCCTGGCAGTAGAGATTCGGAACGCTTTTTGCTTGGGAATTGGTTCTTTCTGGAGGGTTCCTGCAGAGGCTCTGCAACTTGGCCCGAGGAAAACCTTCCTCAGTGGGATTCACCAGTGGCAGGAGAGGAAGATGATCACTGGCAATACAAAGACCATCAACACCATCACCACCACAATAAAGCTCACAACCACTTGACGAAATCAGAAACGTGGGGGCTTGTCATTGTAACTGCCGGATGGGATGGAAAAATCAGGACATTCCATAACTATGGACTGCCGGTCAGTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4

Protein Analysis

677

Amino Acids

76.2

Weight (kDa)

5.65

Isoelectric Point (pI)

51.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 218 - 393 9.6e-16 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 222 - 269 2.2e-07 THOC3 beta-propeller domain
WD40 PF00400 234 - 266 2.3e-09 WD domain, G-beta repeat
Beta-prop_IFT122_1st PF23381 292 - 435 2.3e-07 IFT122 first beta-propeller
WD40_CDC20-Fz PF24807 303 - 477 1.8e-11 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 306 - 386 8.1e-12 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 307 - 539 1.5e-21 Prp19 WD40 domain
WD40 PF00400 308 - 343 2.5e-08 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 309 - 527 1.9e-34 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 309 - 353 1.3e-06 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 311 - 370 1.6e-08 WDR36/Utp21 second beta-propeller domain
WDR55 PF24796 312 - 412 6.2e-06 WDR55
Beta-prop_THOC3 PF25174 316 - 550 3.8e-26 THOC3 beta-propeller domain
Beta-prop_Aladin PF25460 318 - 412 6.5e-06 Aladin seven-bladed propeller
Beta-prop_WDR3_2nd PF25172 319 - 536 7.6e-13 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 320 - 427 3.2e-10 G protein beta WD-40 repeat protein
Beta-prop_WDR90_POC16_2nd PF23393 328 - 446 3.8e-10 WDR90/POC16, second beta-propeller
WD40 PF00400 349 - 385 1.8e-06 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 359 - 539 2e-09 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 370 - 536 1.8e-14 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 390 - 539 5.4e-09 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000109)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g11460 FvH4_1g20081 FvH4_1g21331 FvH4_1g22022 FvH4_1g27932 FvH4_2g04010 FvH4_2g07601 FvH4_2g09181 FvH4_2g18601 FvH4_2g24951 FvH4_2g27371 FvH4_3g15420 FvH4_3g26790 FvH4_3g26791 FvH4_3g34441 FvH4_3g34930 FvH4_3g45133 FvH4_4g02561 FvH4_4g05731 FvH4_4g05732 FvH4_4g06570 FvH4_4g09962 FvH4_4g13551 FvH4_4g13621 FvH4_4g14830 FvH4_4g15081 FvH4_4g26661 FvH4_5g05871 FvH4_5g08822 FvH4_5g12461 FvH4_5g24922 FvH4_5g30051 FvH4_5g30200 FvH4_5g30551 FvH4_5g32991 FvH4_5g36132 FvH4_5g37291 FvH4_6g07931 FvH4_6g08641 FvH4_6g12800 FvH4_6g13840 FvH4_6g13900 FvH4_6g16432 FvH4_6g16701 FvH4_6g28831 FvH4_6g31171 FvH4_6g41261 FvH4_7g00030 FvH4_7g01201 FvH4_7g08120 FvH4_7g10071 FvH4_7g18780
rosa_chinensis RchiOBHm_Chr2g0119561 RchiOBHm_Chr3g0458511 RchiOBHm_Chr3g0477731 RchiOBHm_Chr3g0479111 RchiOBHm_Chr3g0497091 RchiOBHm_Chr5g0072451 RchiOBHm_Chr6g0284681 RchiOBHm_Chr6g0311691 RchiOBHm_Chr7g0182191
rosa_laevigata RLG00000000983 RLG00000001598 RLG00000004616 RLG00000004931 RLG00000006806 RLG00000008371 RLG00000009544 RLG00000009718 RLG00000013465 RLG00000013533 RLG00000014631 RLG00000014987 RLG00000015588 RLG00000016226 RLG00000018171 RLG00000018433 RLG00000018649 RLG00000018710 RLG00000018870 RLG00000019205 RLG00000020638 RLG00000023424 RLG00000024707 RLG00000025967 RLG00000030020 RLG00000030596 RLG00000030996 RLG00000033451 RLG00000033757 RLG00000033758 RLG00000033808 RLG00000033812 RLG00000034857 RLG00000035240 RLG00000035691 RLG00000036232 RLG00000036301 RLG00000036412
rosa_multiflora Rmu_co8113544.1_g000001 Rmu_co8372331.1_g000001 Rmu_sc0000298.1_g000003 Rmu_sc0000552.1_g000026 Rmu_sc0000814.1_g000037 Rmu_sc0001211.1_g000126 Rmu_sc0001311.1_g000026 Rmu_sc0001354.1_g000009 Rmu_sc0002119.1_g000029 Rmu_sc0002357.1_g000020 Rmu_sc0002573.1_g000014 Rmu_sc0002586.1_g000022 Rmu_sc0003517.1_g000004 Rmu_sc0003558.1_g000001 Rmu_sc0004084.1_g000044 Rmu_sc0004143.1_g000011 Rmu_sc0004514.1_g000002 Rmu_sc0005370.1_g000004 Rmu_sc0005392.1_g000015 Rmu_sc0005664.1_g000009 Rmu_sc0006235.1_g000009 Rmu_sc0006423.1_g000001 Rmu_sc0006838.1_g000018 Rmu_sc0006847.1_g000005 Rmu_sc0006959.1_g000019 Rmu_sc0007111.1_g000002 Rmu_sc0009125.1_g000001 Rmu_sc0009546.1_g000015 Rmu_sc0009670.1_g000006 Rmu_sc0011618.1_g000004 Rmu_sc0012306.1_g000002 Rmu_sc0012306.1_g000003 Rmu_sc0012355.1_g000001 Rmu_sc0012355.1_g000002 Rmu_sc0013116.1_g000002 Rmu_sc0016872.1_g000001 Rmu_sc0017043.1_g000005 Rmu_sc0017081.1_g000003 Rmu_sc0018075.1_g000002 Rmu_sc0020578.1_g000002 Rmu_sc0034515.1_g000001 Rmu_sc0040072.1_g000003 Rmu_ssc0000309.1_g000056 Rmu_ssc0000330.1_g000048
rosa_roxburghii Rroxscaffold_1G00007410 Rroxscaffold_1G00027350 Rroxscaffold_1G00043810 Rroxscaffold_2G00099880 Rroxscaffold_2G00112650 Rroxscaffold_2G00122020 Rroxscaffold_3G00223350 Rroxscaffold_3G00239670 Rroxscaffold_5G00333740 Rroxscaffold_5G00335340 Rroxscaffold_5G00341030 Rroxscaffold_5G00342730 Rroxscaffold_6G00426030 Rroxscaffold_7G00162470 Rroxscaffold_7G00196110 Rroxscaffold_7G00202440 Rroxscaffold_7G00202450 Rroxscaffold_7G00215740
rosa_rugosa Rorug01G0176600 Rorug01G0276600 Rorug02G0236500 Rorug02G0277000 Rorug02G0363300 Rorug02G0375900 Rorug02G0473700 Rorug02G0546200 Rorug02G0601000 Rorug03G0158400 Rorug03G0289500 Rorug04G0026600 Rorug04G0090000 Rorug04G0105500 Rorug04G0263100 Rorug04G0394500 Rorug05G0122200 Rorug05G0152200 Rorug05G0541800 Rorug05G0551100 Rorug05G0554400 Rorug06G0244900 Rorug06G0401400
rosa_samantha Rh1CG158000 Rh1CG280800 Rh1CG326900 Rh2BG051500 Rh2BG101500 Rh2BG438200 Rh2BG509100 Rh2BG531600 Rh2BG531800 Rh2BG629800 Rh2BG629900 Rh2CG565300 Rh2DG448000 Rh2DG540600 Rh2DG540700 Rh2DG641700 Rh2DG641800 Rh3AG222200 Rh3BG034200 Rh3BG254700 Rh3DG286800 Rh3DG322600 Rh4AG009200 Rh4AG050400 Rh4AG223100 Rh4BG072500 Rh4BG106000 Rh4CG010200 Rh4CG053700 Rh4CG236100 Rh4CG236200 Rh4CG236300 Rh4DG104800 Rh5AG474800 Rh5BG214500 Rh5BG360000 Rh5BG403700 Rh5BG494900 Rh5DG255300 Rh6AG205400 Rh6BG227300 Rh6CG280700 Rh6DG273400 Rh7AG187300 Rh7AG220800 Rh7AG355700 Rh7AG462000 Rh7AG462200 Rh7AG465900 Rh7CG198300 Rh7CG222100 Rh7CG373000 Rh7CG479300 Rh7DG233800
rosa_wichuraiana Rw0G018350 Rw1G026500 Rw2G024750 Rw2G051200 Rw5G001570 Rw5G036850 Rw6G016070 Rw6G042710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 560
AasI GACNNNNNNGTC 1 cut(s) 1421
Acc16I TGCGCA 1 cut(s) 791
Acc36I ACCTGC 1 cut(s) 393
Acc65I GGTACC 1 cut(s) 221
AccB1I GGYRCC 1 cut(s) 221
AccII CGCG 2 cut(s) 1546, 1548
AciI CCGC 3 cut(s) 45, 1270, 1512
AclWI GGATC 4 cut(s) 187, 1122, 1135, 1605
AcoI YGGCCR 2 cut(s) 751, 943
AcsI RAATTY 4 cut(s) 355, 400, 702, 979
AcuI CTGAAG 4 cut(s) 84, 516, 639, 1286
AdeI CACNNNGTG 1 cut(s) 125
AfaI GTAC 3 cut(s) 223, 426, 668
AfiI CCNNNNNNNGG 3 cut(s) 1478, 1661, 1862
AflIII ACRYGT 1 cut(s) 1054
AgsI TTSAA 5 cut(s) 112, 516, 917, 984, 1096
AhdI GACNNNNNGTC 1 cut(s) 2021
AjnI CCWGG 2 cut(s) 966, 1744
AjuI GAANNNNNNNTTGG 4 cut(s) 74, 106, 201, 233
Alw21I GWGCWC 1 cut(s) 284
Alw26I GTCTC 4 cut(s) 244, 375, 598, 995
AlwI GGATC 4 cut(s) 187, 1122, 1135, 1605
Ama87I CYCGRG 2 cut(s) 277, 1826
AoxI GGCC 5 cut(s) 523, 751, 943, 1726, 1823
ApeKI GCWGC 3 cut(s) 272, 288, 680
ApoI RAATTY 4 cut(s) 355, 400, 702, 979
Asp700I GAANNNNTTC 5 cut(s) 469, 1238, 1299, 1784, 1836
Asp718I GGTACC 1 cut(s) 221
AspLEI GCGC 2 cut(s) 792, 1548
AspS9I GGNCC 2 cut(s) 523, 1824
AsuC2I CCSGG 1 cut(s) 694
AsuHPI GGTGA 5 cut(s) 785, 915, 1351, 1845, 1901
AsuII TTCGAA 1 cut(s) 910
AvaI CYCGRG 2 cut(s) 277, 1826
BaeI ACNNNNGTAYC 6 cut(s) 205, 238, 606, 606, 639, 639
BalI TGGCCA 2 cut(s) 753, 945
BamHI GGATCC 1 cut(s) 1127
BanI GGYRCC 1 cut(s) 221
BanII GRGCYC 1 cut(s) 284
BbsI GAAGAC 2 cut(s) 1419, 1460
Bbv12I GWGCWC 1 cut(s) 284
BbvI GCAGC 3 cut(s) 259, 300, 667
BceAI ACGGC 1 cut(s) 170
BcgI CGANNNNNNTGC 2 cut(s) 1367, 1401
BciT130I CCWGG 2 cut(s) 968, 1746
BciVI GTATCC 1 cut(s) 634
BclI TGATCA 1 cut(s) 1876
BcnI CCSGG 1 cut(s) 694
BcoDI GTCTC 4 cut(s) 244, 375, 598, 995
BfaI CTAG 4 cut(s) 300, 350, 1332, 1698
BfmI CTRYAG 2 cut(s) 416, 1804
BfuAI ACCTGC 1 cut(s) 393
BfuI GTATCC 1 cut(s) 634
BglII AGATCT 2 cut(s) 1341, 1444
BisI GCNGC 4 cut(s) 273, 289, 681, 1270
BlsI GCNGC 4 cut(s) 274, 290, 682, 1271
Bme1390I CCNGG 3 cut(s) 694, 968, 1746
BmeRI GACNNNNNGTC 1 cut(s) 2021
BmeT110I CYCGRG 2 cut(s) 277, 1826
BmgT120I GGNCC 2 cut(s) 523, 1824
BmiI GGNNCC 4 cut(s) 223, 1129, 1252, 1801
BmrFI CCNGG 3 cut(s) 694, 968, 1746
BmrI ACTGGG 1 cut(s) 1192
BmsI GCATC 2 cut(s) 802, 1374
BmuI ACTGGG 1 cut(s) 1192
BpiI GAAGAC 2 cut(s) 1419, 1460
BpmI CTGGAG 1 cut(s) 1814
Bpu14I TTCGAA 1 cut(s) 910
BpuEI CTTGAG 1 cut(s) 822
BpuMI CCSGG 1 cut(s) 694
BsaI GGTCTC 1 cut(s) 375
BsaJI CCNNGG 4 cut(s) 1005, 1377, 1655, 1827
BsaXI ACNNNNNCTCC 6 cut(s) 80, 110, 568, 573, 598, 603
Bsc4I CCNNNNNNNGG 3 cut(s) 1478, 1661, 1862
Bse118I RCCGGY 2 cut(s) 1361, 2020
Bse1I ACTGG 6 cut(s) 772, 951, 1187, 1369, 1856, 1886
Bse3DI GCAATG 1 cut(s) 759
BseBI CCWGG 2 cut(s) 968, 1746
BseDI CCNNGG 4 cut(s) 1005, 1377, 1655, 1827
BseGI GGATG 6 cut(s) 884, 1365, 1505, 1586, 1985, 1990
BseLI CCNNNNNNNGG 3 cut(s) 1478, 1661, 1862
BseMI GCAATG 1 cut(s) 759
BseMII CTCAG 7 cut(s) 465, 470, 477, 609, 1155, 1542, 1856
BseNI ACTGG 6 cut(s) 772, 951, 1187, 1369, 1856, 1886
BseRI GAGGAG 5 cut(s) 101, 283, 286, 589, 594
BseXI GCAGC 3 cut(s) 259, 300, 667
BsgI GTGCAG 1 cut(s) 666
Bsh1236I CGCG 2 cut(s) 1546, 1548
BshFI GGCC 5 cut(s) 525, 753, 945, 1728, 1825
BshNI GGYRCC 1 cut(s) 221
BsiHKAI GWGCWC 1 cut(s) 284
BsiHKCI CYCGRG 2 cut(s) 277, 1826
BsiSI CCGG 5 cut(s) 526, 693, 1362, 1977, 2021
BslFI GGGAC 3 cut(s) 227, 356, 1574
BslI CCNNNNNNNGG 3 cut(s) 1478, 1661, 1862
BsmAI GTCTC 4 cut(s) 244, 375, 598, 995
BsmBI CGTCTC 1 cut(s) 995
BsmFI GGGAC 3 cut(s) 227, 356, 1574
BsmI GAATGC 2 cut(s) 509, 1086
BsnI GGCC 5 cut(s) 525, 753, 945, 1728, 1825
Bso31I GGTCTC 1 cut(s) 375
BsoBI CYCGRG 2 cut(s) 277, 1826
Bsp119I TTCGAA 1 cut(s) 910
Bsp1286I GDGCHC 1 cut(s) 284
Bsp143I GATC 8 cut(s) 179, 645, 867, 1127, 1341, 1444, 1610, 1876
Bsp19I CCATGG 1 cut(s) 1005
BspACI CCGC 3 cut(s) 45, 1270, 1512
BspANI GGCC 5 cut(s) 525, 753, 945, 1728, 1825
BspCNI CTCAG 7 cut(s) 466, 469, 478, 608, 1156, 1541, 1855
BspFNI CGCG 2 cut(s) 1546, 1548
BspLI GGNNCC 4 cut(s) 223, 1129, 1252, 1801
BspMAI CTGCAG 1 cut(s) 1808
BspMI ACCTGC 1 cut(s) 393
BspPI GGATC 4 cut(s) 187, 1122, 1135, 1605
BspQI GCTCTTC 1 cut(s) 1305
BspT104I TTCGAA 1 cut(s) 910
BspT107I GGYRCC 1 cut(s) 221
BspTNI GGTCTC 1 cut(s) 375
BsrDI GCAATG 1 cut(s) 759
BsrFI RCCGGY 2 cut(s) 1361, 2020
BsrI ACTGG 6 cut(s) 772, 951, 1187, 1369, 1856, 1886
BssAI RCCGGY 2 cut(s) 1361, 2020
BssECI CCNNGG 4 cut(s) 1005, 1377, 1655, 1827
BssMI GATC 8 cut(s) 179, 645, 867, 1127, 1341, 1444, 1610, 1876
BssT1I CCWWGG 2 cut(s) 1005, 1655
Bst2UI CCWGG 2 cut(s) 968, 1746
Bst4CI ACNGT 6 cut(s) 420, 555, 659, 905, 989, 1018
Bst6I CTCTTC 4 cut(s) 60, 530, 912, 1305
BstAPI GCANNNNNTGC 1 cut(s) 1047
BstBI TTCGAA 1 cut(s) 910
BstC8I GCNNGC 1 cut(s) 1261
BstDEI CTNAG 9 cut(s) 5, 456, 474, 486, 595, 847, 1164, 1528, 1842
BstDSI CCRYGG 1 cut(s) 1005
BstENI CCTNNNNNAGG 1 cut(s) 1659
BstF5I GGATG 6 cut(s) 884, 1365, 1505, 1586, 1985, 1990
BstFNI CGCG 2 cut(s) 1546, 1548
BstHHI GCGC 2 cut(s) 792, 1548
BstKTI GATC 8 cut(s) 182, 648, 870, 1130, 1344, 1447, 1613, 1879
BstMAI GTCTC 4 cut(s) 244, 375, 598, 995
BstMBI GATC 8 cut(s) 179, 645, 867, 1127, 1341, 1444, 1610, 1876
BstMWI GCNNNNNNNGC 3 cut(s) 288, 1047, 1269
BstNI CCWGG 2 cut(s) 968, 1746
BstNSI RCATGY 2 cut(s) 1058, 1086
BstSCI CCNGG 3 cut(s) 692, 966, 1744
BstSFI CTRYAG 2 cut(s) 416, 1804
BstUI CGCG 2 cut(s) 1546, 1548
BstV1I GCAGC 3 cut(s) 259, 300, 667
BstV2I GAAGAC 2 cut(s) 1419, 1460
BstX2I RGATCY 4 cut(s) 179, 1127, 1341, 1444
BstXI CCANNNNNNTGG 1 cut(s) 2012
BstYI RGATCY 4 cut(s) 179, 1127, 1341, 1444
BsuI GTATCC 1 cut(s) 634
BsuRI GGCC 5 cut(s) 525, 753, 945, 1728, 1825
BtgI CCRYGG 1 cut(s) 1005
BtsCI GGATG 6 cut(s) 884, 1365, 1505, 1586, 1985, 1990
BtsIMutI CAGTG 7 cut(s) 78, 958, 1052, 1129, 1850, 1863, 1879
BveI ACCTGC 1 cut(s) 393
Cac8I GCNNGC 1 cut(s) 1261
CfoI GCGC 2 cut(s) 792, 1548
Cfr10I RCCGGY 2 cut(s) 1361, 2020
Cfr13I GGNCC 2 cut(s) 523, 1824
CseI GACGC 1 cut(s) 1745
Csp6I GTAC 3 cut(s) 222, 425, 667
CviAII CATG 5 cut(s) 670, 1006, 1055, 1083, 1666
CviQI GTAC 3 cut(s) 222, 425, 667
DdeI CTNAG 9 cut(s) 5, 456, 474, 486, 595, 847, 1164, 1528, 1842
DpnI GATC 8 cut(s) 181, 647, 869, 1129, 1343, 1446, 1612, 1878
DpnII GATC 8 cut(s) 179, 645, 867, 1127, 1341, 1444, 1610, 1876
DraIII CACNNNGTG 1 cut(s) 125
DrdI GACNNNNNNGTC 1 cut(s) 1421
DriI GACNNNNNGTC 1 cut(s) 2021
DseDI GACNNNNNNGTC 1 cut(s) 1421
EaeI YGGCCR 2 cut(s) 751, 943
Eam1104I CTCTTC 4 cut(s) 60, 530, 912, 1305
Eam1105I GACNNNNNGTC 1 cut(s) 2021
EarI CTCTTC 4 cut(s) 60, 530, 912, 1305
Ecl136II GAGCTC 1 cut(s) 282
Eco130I CCWWGG 2 cut(s) 1005, 1655
Eco24I GRGCYC 1 cut(s) 284
Eco31I GGTCTC 1 cut(s) 375
Eco53kI GAGCTC 1 cut(s) 282
Eco57I CTGAAG 4 cut(s) 84, 516, 639, 1286
Eco88I CYCGRG 2 cut(s) 277, 1826
EcoICRI GAGCTC 1 cut(s) 282
EcoNI CCTNNNNNAGG 1 cut(s) 1659
EcoRII CCWGG 2 cut(s) 966, 1744
EcoT14I CCWWGG 2 cut(s) 1005, 1655
EcoT22I ATGCAT 1 cut(s) 1088
EcoT38I GRGCYC 1 cut(s) 284
ErhI CCWWGG 2 cut(s) 1005, 1655
Esp3I CGTCTC 1 cut(s) 995
FaeI CATG 5 cut(s) 673, 1009, 1058, 1086, 1669
FalI AAGNNNNNCTT 4 cut(s) 924, 956, 1284, 1316
FaqI GGGAC 3 cut(s) 227, 356, 1574
FatI CATG 5 cut(s) 669, 1005, 1054, 1082, 1665
FauI CCCGC 1 cut(s) 1505
FbaI TGATCA 1 cut(s) 1876
Fnu4HI GCNGC 4 cut(s) 273, 289, 681, 1270
FokI GGATG 6 cut(s) 891, 1352, 1492, 1573, 1992, 1997
FriOI GRGCYC 1 cut(s) 284
Fsp4HI GCNGC 4 cut(s) 273, 289, 681, 1270
FspAI RTGCGCAY 1 cut(s) 791
FspBI CTAG 4 cut(s) 300, 350, 1332, 1698
FspI TGCGCA 1 cut(s) 791
GlaI GCGC 2 cut(s) 791, 1547
GluI GCNGC 4 cut(s) 273, 289, 681, 1270
GsuI CTGGAG 1 cut(s) 1814
HaeIII GGCC 5 cut(s) 525, 753, 945, 1728, 1825
HapII CCGG 5 cut(s) 526, 693, 1362, 1977, 2021
HgaI GACGC 1 cut(s) 1745
HhaI GCGC 2 cut(s) 792, 1548
Hin1II CATG 5 cut(s) 673, 1009, 1058, 1086, 1669
Hin6I GCGC 2 cut(s) 790, 1546
HinP1I GCGC 2 cut(s) 790, 1546
HincII GTYRAC 1 cut(s) 1180
HindII GTYRAC 1 cut(s) 1180
HpaII CCGG 5 cut(s) 526, 693, 1362, 1977, 2021
HphI GGTGA 5 cut(s) 785, 915, 1351, 1845, 1901
Hpy166II GTNNAC 2 cut(s) 1180, 1703
Hpy8I GTNNAC 2 cut(s) 1180, 1703
HpyAV CCTTC 8 cut(s) 19, 76, 124, 332, 1228, 1329, 1628, 1847
HpyCH4III ACNGT 6 cut(s) 420, 555, 659, 905, 989, 1018
HpyCH4IV ACGT 3 cut(s) 216, 1519, 1952
HpyF10VI GCNNNNNNNGC 3 cut(s) 288, 1047, 1269
HpyF3I CTNAG 9 cut(s) 5, 456, 474, 486, 595, 847, 1164, 1528, 1842
HpySE526I ACGT 3 cut(s) 216, 1519, 1952
Hsp92II CATG 5 cut(s) 673, 1009, 1058, 1086, 1669
HspAI GCGC 2 cut(s) 790, 1546
KpnI GGTACC 1 cut(s) 225
Ksp22I TGATCA 1 cut(s) 1876
Kzo9I GATC 8 cut(s) 179, 645, 867, 1127, 1341, 1444, 1610, 1876
LguI GCTCTTC 1 cut(s) 1305
LmnI GCTCC 2 cut(s) 269, 296
Lsp1109I GCAGC 3 cut(s) 259, 300, 667
LweI GCATC 2 cut(s) 802, 1374
MaeI CTAG 4 cut(s) 300, 350, 1332, 1698
MaeII ACGT 3 cut(s) 216, 1519, 1952
MaeIII GTNAC 5 cut(s) 302, 802, 921, 1078, 1969
MalI GATC 8 cut(s) 181, 647, 869, 1129, 1343, 1446, 1612, 1878
MboI GATC 8 cut(s) 179, 645, 867, 1127, 1341, 1444, 1610, 1876
MflI RGATCY 4 cut(s) 179, 1127, 1341, 1444
MhlI GDGCHC 1 cut(s) 284
MlsI TGGCCA 2 cut(s) 753, 945
MluNI TGGCCA 2 cut(s) 753, 945
MlyI GAGTC 4 cut(s) 77, 929, 1409, 1535
MmeI TCCRAC 2 cut(s) 72, 1513
Mox20I TGGCCA 2 cut(s) 753, 945
Mph1103I ATGCAT 1 cut(s) 1088
MroXI GAANNNNTTC 5 cut(s) 469, 1238, 1299, 1784, 1836
MscI TGGCCA 2 cut(s) 753, 945
MseI TTAA 2 cut(s) 861, 1452
MslI CAYNNNNRTG 3 cut(s) 951, 1068, 2010
Msp20I TGGCCA 2 cut(s) 753, 945
MspA1I CMGCKG 2 cut(s) 1512, 1572
MspI CCGG 5 cut(s) 526, 693, 1362, 1977, 2021
MspR9I CCNGG 3 cut(s) 694, 968, 1746
Mva1269I GAATGC 2 cut(s) 509, 1086
MvaI CCWGG 2 cut(s) 968, 1746
MvnI CGCG 2 cut(s) 1546, 1548
MwoI GCNNNNNNNGC 3 cut(s) 288, 1047, 1269
NciI CCSGG 1 cut(s) 694
NcoI CCATGG 1 cut(s) 1005
NdeII GATC 8 cut(s) 179, 645, 867, 1127, 1341, 1444, 1610, 1876
NlaIII CATG 5 cut(s) 673, 1009, 1058, 1086, 1669
NlaIV GGNNCC 4 cut(s) 223, 1129, 1252, 1801
NmuCI GTSAC 2 cut(s) 302, 921
NsbI TGCGCA 1 cut(s) 791
NsiI ATGCAT 1 cut(s) 1088
NspI RCATGY 2 cut(s) 1058, 1086
NspV TTCGAA 1 cut(s) 910
PaeR7I CTCGAG 1 cut(s) 277
PciI ACATGT 1 cut(s) 1054
PciSI GCTCTTC 1 cut(s) 1305
PctI GAATGC 2 cut(s) 509, 1086
PdmI GAANNNNTTC 5 cut(s) 469, 1238, 1299, 1784, 1836
PfeI GAWTC 6 cut(s) 41, 173, 1283, 1318, 1757, 1850
PkrI GCNGC 4 cut(s) 274, 290, 682, 1271
PleI GAGTC 4 cut(s) 76, 928, 1409, 1535
PpsI GAGTC 4 cut(s) 76, 928, 1409, 1535
PscI ACATGT 1 cut(s) 1054
PsiI TTATAA 1 cut(s) 560
Psp124BI GAGCTC 1 cut(s) 284
Psp6I CCWGG 2 cut(s) 966, 1744
PspGI CCWGG 2 cut(s) 966, 1744
PspN4I GGNNCC 4 cut(s) 223, 1129, 1252, 1801
PspPI GGNCC 2 cut(s) 523, 1824
PspXI VCTCGAGB 1 cut(s) 277
PsrI GAACNNNNNNTAC 2 cut(s) 436, 468
PstI CTGCAG 1 cut(s) 1808
PsuI RGATCY 4 cut(s) 179, 1127, 1341, 1444
PvuII CAGCTG 1 cut(s) 1572
RsaI GTAC 3 cut(s) 223, 426, 668
RsaNI GTAC 3 cut(s) 222, 425, 667
RseI CAYNNNNRTG 3 cut(s) 951, 1068, 2010
SacI GAGCTC 1 cut(s) 284
SapI GCTCTTC 1 cut(s) 1305
SaqAI TTAA 2 cut(s) 861, 1452
SatI GCNGC 4 cut(s) 273, 289, 681, 1270
Sau3AI GATC 8 cut(s) 179, 645, 867, 1127, 1341, 1444, 1610, 1876
Sau96I GGNCC 2 cut(s) 523, 1824
SchI GAGTC 4 cut(s) 77, 929, 1409, 1535
ScrFI CCNGG 3 cut(s) 694, 968, 1746
SduI GDGCHC 1 cut(s) 284
SfaNI GCATC 2 cut(s) 802, 1374
SfcI CTRYAG 2 cut(s) 416, 1804
Sfr274I CTCGAG 1 cut(s) 277
SfuI TTCGAA 1 cut(s) 910
SlaI CTCGAG 1 cut(s) 277
SmiMI CAYNNNNRTG 3 cut(s) 951, 1068, 2010
SmlI CTYRAG 2 cut(s) 277, 837
SmoI CTYRAG 2 cut(s) 277, 837
SsiI CCGC 3 cut(s) 45, 1270, 1512
SspI AATATT 1 cut(s) 317
SspMI CTAG 4 cut(s) 300, 350, 1332, 1698
SstI GAGCTC 1 cut(s) 284
StyD4I CCNGG 3 cut(s) 692, 966, 1744
StyI CCWWGG 2 cut(s) 1005, 1655
TaaI ACNGT 6 cut(s) 420, 555, 659, 905, 989, 1018
TaiI ACGT 3 cut(s) 219, 1522, 1955
TaqI TCGA 7 cut(s) 278, 434, 883, 910, 1104, 1147, 1609
TatI WGTACW 1 cut(s) 424
TauI GCSGC 1 cut(s) 1272
TfiI GAWTC 6 cut(s) 41, 173, 1283, 1318, 1757, 1850
Tru1I TTAA 2 cut(s) 861, 1452
Tru9I TTAA 2 cut(s) 861, 1452
TscAI CASTG 7 cut(s) 78, 958, 1052, 1129, 1850, 1863, 1886
TseFI GTSAC 2 cut(s) 302, 921
TseI GCWGC 3 cut(s) 272, 288, 680
Tsp45I GTSAC 2 cut(s) 302, 921
TspDTI ATGAA 7 cut(s) 317, 384, 656, 749, 1108, 1293, 1310
TspGWI ACGGA 1 cut(s) 1415
TspRI CASTG 7 cut(s) 78, 958, 1052, 1129, 1850, 1863, 1886
XagI CCTNNNNNAGG 1 cut(s) 1659
XapI RAATTY 4 cut(s) 355, 400, 702, 979
XbaI TCTAGA 1 cut(s) 1331
XceI RCATGY 2 cut(s) 1058, 1086
XcmI CCANNNNNNNNNTGG 1 cut(s) 938
XhoI CTCGAG 1 cut(s) 277
XmnI GAANNNNTTC 5 cut(s) 469, 1238, 1299, 1784, 1836
XspI CTAG 4 cut(s) 300, 350, 1332, 1698
Zsp2I ATGCAT 1 cut(s) 1088
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.